MD14G1141700.v1.1

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001359
Physical Location & Seq
Reverse (-)
23424423 .. 23431017
6595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1141700.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGAGCAAATCACAAGGTTTCTCGTGGGCACGTCTCATCGTGAAACAAGGCATTAAGAAACCCAGCAAAGCCCCACTCCCCTTGGCCCGGAAAATCTCCACTCGGCCACACTTTCCCGCCACCCCTCAGCCACCGAAATGGTCGTTTTCTAGCAAGATCCCTTATTCAACAGTGTCATCCAGAGCGGCCCAAGACCTTCTGGGGGAGGTGGAGAGAGAAAAGCAGAGAGAACGAGAGCAGAGGAAGAGGGTGGGGCTGGACACCAAAGACATTGACCGAGAAGATGAGGAGGACTACATGGGCGTTATGCCATTGATTGAGAAGCTCGAGGAGGAGAAGCTCAAGGACACCGGAGACTTGAACTTGTACGAGGAGCCCACCGATTCCGATAGTGACGAAGACGATGAGAGGTTCACTCCTGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAGGAAGTTCACGCGCCACGAGGAGTTGCTCAACAACTTCACCGAGGCTGAGACACTTGATGATGCTTATAAATGGATGAATAAAATTGACAAGTTTGAGCAAAAACATTTCCGCCTTCGCCCCGAATATAGGGTCATTGGTGAGTTGATGAACCGTCTCAAAGTAGCTGAGGGAAAGGACAAATTCATCCTCCAGCAGAAACTAAACAGGGCTATGAGATTGGTGCAGTGGAAGGAAGCCTATGATCCAAACAATCCTGCCAATTATGGAGTCATCCAACATGAACAAGTGGAGCCCTCAGTTGATTTATTGGAACATGCTGGGTTTGAAAAACAAAAGCAAATTATACAAGGAGTTGACGACGACAACGAAGAAGAATTTAATGACATGAAGGAGAAAGATGACATGCTGTTAGAGAAGCTTAATGCCATTGACAAAAAACTCGAAGAGAAGCTAGCAGAGTTAGATCATACATTCGGGAAGAAGGGTAAGGTTCTAGAGGAAGAGATCAGAGACCTAGCAGAGGAAAGAAATGATTTGACAGAGAAGAAGAGGAGACCTCTCTACAGGAAAGGGTTTGATGTTAAATTAATCAATGTGAACCGAACTTGTAAAGTAACAAAGGGGGGGCAAGTGGTCAAGTACACTGCCATATTAGCTTGTGGGAATTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGCCCAGCAGTCCCTATCGCTCTTCAGAAGATGGATCTCATGCATATGAGAAATGCTTTCAGAATCTCCAACTACATTGAACGACACGAGGAGCATACAATTGCGCATGCAATACAAACAAGTTATAAAAAAACAAAGGTGTATCTCTGGCCTGCACCCACTAGGACAGGTATGAAAGCAGGAAGAACTGTTGAAACCATTCTAAACTTAGCTGGTTTCAAAAATGTTAAGTCTAAGGTTGTAGGCTCAAGGAATCCTTATAACACAGTCAAGGCTCTCTTCAAATCCCTTAATGCGATTGAAACTCGAAAGGATGTTCAAGATAAGTTTGGCCGCACTGTTGTTGAGAAGTACCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

506

Amino Acids

58.68

Weight (kDa)

8.94

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 344 - 395 9.5e-13 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 427 - 493 5.1e-21 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 522, 1281, 1416
Acc16I TGCGCA 1 cut(s) 1260
AccBSI CCGCTC 1 cut(s) 185
AccII CGCG 1 cut(s) 466
AciI CCGC 4 cut(s) 117, 185, 565, 1489
AclWI GGATC 3 cut(s) 151, 692, 1197
AcoI YGGCCR 2 cut(s) 104, 1486
AcsI RAATTY 2 cut(s) 635, 830
AcuI CTGAAG 1 cut(s) 1163
AfaI GTAC 3 cut(s) 368, 1097, 1508
AfiI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
AgsI TTSAA 9 cut(s) 168, 361, 782, 1235, 1349, 1375, 1438, 1457, 1475
AjiI CACGTC 1 cut(s) 32
AluBI AGCT 7 cut(s) 325, 340, 620, 874, 907, 1112, 1367
AluI AGCT 7 cut(s) 325, 340, 620, 874, 907, 1112, 1367
Alw26I GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
AlwI GGATC 3 cut(s) 151, 692, 1197
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 6 cut(s) 84, 104, 186, 1156, 1304, 1486
ApoI RAATTY 2 cut(s) 635, 830
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
AseI ATTAAT 1 cut(s) 1043
Asp700I GAANNNNTTC 1 cut(s) 1353
AspLEI GCGC 2 cut(s) 468, 1261
AspS9I GGNCC 3 cut(s) 85, 187, 1157
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 484, 605
AsuNHI GCTAGC 1 cut(s) 907
AvaI CYCGRG 1 cut(s) 326
BaeGI GKGCMC 1 cut(s) 31
BanII GRGCYC 2 cut(s) 378, 750
BauI CACGAG 3 cut(s) 22, 470, 1241
BbsI GAAGAC 1 cut(s) 405
BbvCI CCTCAGC 2 cut(s) 126, 621
BccI CCATC 1 cut(s) 1180
BceAI ACGGC 1 cut(s) 410
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BfaI CTAG 5 cut(s) 150, 908, 950, 971, 1317
BfmI CTRYAG 1 cut(s) 1018
BisI GCNGC 2 cut(s) 186, 1489
BlsI GCNGC 2 cut(s) 187, 1490
Bme1390I CCNGG 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 85, 187, 1157
BmiI GGNNCC 2 cut(s) 375, 747
BmrFI CCNGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 412, 505
BmtI GCTAGC 1 cut(s) 911
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 4 cut(s) 400, 432, 997, 1029
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 2 cut(s) 126, 621
BpuEI CTTGAG 2 cut(s) 326, 1387
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 2 cut(s) 960, 1003
BsaJI CCNNGG 2 cut(s) 81, 495
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BseDI CCNNGG 2 cut(s) 81, 495
BseGI GGATG 5 cut(s) 176, 534, 639, 726, 1474
BseLI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BseMII CTCAG 4 cut(s) 140, 492, 612, 765
BseRI GAGGAG 7 cut(s) 302, 344, 347, 386, 488, 1021, 1259
BseSI GKGCMC 1 cut(s) 31
BseYI CCCAGC 3 cut(s) 62, 773, 1159
BsgI GTGCAG 2 cut(s) 698, 1293
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 6 cut(s) 86, 106, 188, 1158, 1306, 1488
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 2 cut(s) 88, 351
BslFI GGGAC 1 cut(s) 1151
BslI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BsmAI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BsmBI CGTCTC 2 cut(s) 38, 614
BsmFI GGGAC 1 cut(s) 1151
BsnI GGCC 6 cut(s) 86, 106, 188, 1158, 1306, 1488
Bso31I GGTCTC 2 cut(s) 960, 1003
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 3 cut(s) 31, 378, 750
Bsp143I GATC 5 cut(s) 156, 697, 919, 960, 1189
BspACI CCGC 4 cut(s) 117, 185, 565, 1489
BspANI GGCC 6 cut(s) 86, 106, 188, 1158, 1306, 1488
BspCNI CTCAG 4 cut(s) 139, 493, 613, 764
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 375, 747
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 3 cut(s) 151, 692, 1197
BspQI GCTCTTC 1 cut(s) 1182
BspTNI GGTCTC 2 cut(s) 960, 1003
BsrBI CCGCTC 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 81, 495
BssMI GATC 5 cut(s) 156, 697, 919, 960, 1189
BssSI CACGAG 3 cut(s) 22, 470, 1241
BssT1I CCWWGG 1 cut(s) 81
Bst2BI CACGAG 3 cut(s) 22, 470, 1241
Bst4CI ACNGT 5 cut(s) 172, 608, 1345, 1423, 1495
Bst6I CTCTTC 7 cut(s) 239, 428, 894, 951, 998, 1182, 1439
BstC8I GCNNGC 3 cut(s) 909, 1263, 1308
BstDEI CTNAG 6 cut(s) 126, 501, 621, 751, 1363, 1389
BstENI CCTNNNNNAGG 1 cut(s) 974
BstF5I GGATG 5 cut(s) 176, 534, 639, 726, 1474
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 2 cut(s) 468, 1261
BstKTI GATC 5 cut(s) 159, 700, 922, 963, 1192
BstMAI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BstMBI GATC 5 cut(s) 156, 697, 919, 960, 1189
BstMWI GCNNNNNNNGC 1 cut(s) 1109
BstNSI RCATGY 3 cut(s) 773, 862, 1265
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 1018
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 2 cut(s) 156, 1189
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 2 cut(s) 156, 1189
BsuRI GGCC 6 cut(s) 86, 106, 188, 1158, 1306, 1488
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 5 cut(s) 176, 534, 639, 726, 1474
BtsI GCAGTG 2 cut(s) 686, 1098
BtsIMutI CAGTG 4 cut(s) 177, 686, 1098, 1491
Cac8I GCNNGC 3 cut(s) 909, 1263, 1308
CfoI GCGC 2 cut(s) 468, 1261
Cfr13I GGNCC 3 cut(s) 85, 187, 1157
Csp6I GTAC 3 cut(s) 367, 1096, 1507
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 8 cut(s) 298, 734, 770, 841, 859, 1127, 1195, 1262
CviQI GTAC 3 cut(s) 367, 1096, 1507
DdeI CTNAG 6 cut(s) 126, 501, 621, 751, 1363, 1389
DpnI GATC 5 cut(s) 158, 699, 921, 962, 1191
DpnII GATC 5 cut(s) 156, 697, 919, 960, 1189
EaeI YGGCCR 2 cut(s) 104, 1486
Eam1104I CTCTTC 7 cut(s) 239, 428, 894, 951, 998, 1182, 1439
EarI CTCTTC 7 cut(s) 239, 428, 894, 951, 998, 1182, 1439
EciI GGCGGA 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 378, 750
Eco31I GGTCTC 2 cut(s) 960, 1003
Eco57I CTGAAG 1 cut(s) 1163
Eco88I CYCGRG 1 cut(s) 326
EcoNI CCTNNNNNAGG 1 cut(s) 974
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 1200
EcoT38I GRGCYC 2 cut(s) 378, 750
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 2 cut(s) 38, 614
FaeI CATG 8 cut(s) 301, 737, 773, 844, 862, 1130, 1198, 1265
FalI AAGNNNNNCTT 4 cut(s) 1396, 1428, 1418, 1450
FaqI GGGAC 1 cut(s) 1151
FatI CATG 8 cut(s) 297, 733, 769, 840, 858, 1126, 1194, 1261
FauI CCCGC 1 cut(s) 124
FauNDI CATATG 1 cut(s) 1200
Fnu4HI GCNGC 2 cut(s) 186, 1489
FokI GGATG 5 cut(s) 163, 541, 626, 713, 1481
FriOI GRGCYC 2 cut(s) 378, 750
Fsp4HI GCNGC 2 cut(s) 186, 1489
FspBI CTAG 5 cut(s) 150, 908, 950, 971, 1317
FspI TGCGCA 1 cut(s) 1260
GlaI GCGC 2 cut(s) 467, 1260
GluI GCNGC 2 cut(s) 186, 1489
GsaI CCCAGC 3 cut(s) 66, 777, 1163
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 6 cut(s) 86, 106, 188, 1158, 1306, 1488
HapII CCGG 2 cut(s) 88, 351
HhaI GCGC 2 cut(s) 468, 1261
Hin1II CATG 8 cut(s) 301, 737, 773, 844, 862, 1130, 1198, 1265
Hin6I GCGC 2 cut(s) 466, 1259
HinP1I GCGC 2 cut(s) 466, 1259
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 4 cut(s) 383, 723, 1218, 1408
HpaII CCGG 2 cut(s) 88, 351
HphI GGTGA 2 cut(s) 484, 605
Hpy166II GTNNAC 5 cut(s) 414, 462, 811, 1054, 1098
Hpy188I TCNGA 5 cut(s) 388, 453, 965, 1182, 1217
Hpy188III TCNNGA 7 cut(s) 40, 180, 419, 430, 931, 950, 1475
Hpy8I GTNNAC 5 cut(s) 414, 462, 811, 1054, 1098
Hpy99I CGWCG 1 cut(s) 818
HpyAV CCTTC 5 cut(s) 206, 578, 679, 838, 931
HpyCH4III ACNGT 5 cut(s) 172, 608, 1345, 1423, 1495
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 4 cut(s) 679, 1198, 1265, 1310
HpyF10VI GCNNNNNNNGC 1 cut(s) 1109
HpyF3I CTNAG 6 cut(s) 126, 501, 621, 751, 1363, 1389
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 8 cut(s) 301, 737, 773, 844, 862, 1130, 1198, 1265
HspAI GCGC 2 cut(s) 466, 1259
Kzo9I GATC 5 cut(s) 156, 697, 919, 960, 1189
LguI GCTCTTC 1 cut(s) 1182
LmnI GCTCC 3 cut(s) 373, 745, 1246
LweI GCATC 2 cut(s) 412, 505
MaeI CTAG 5 cut(s) 150, 908, 950, 971, 1317
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 2 cut(s) 392, 1069
MalI GATC 5 cut(s) 158, 699, 921, 962, 1191
MbiI CCGCTC 1 cut(s) 185
MboI GATC 5 cut(s) 156, 697, 919, 960, 1189
MfeI CAATTG 1 cut(s) 1254
MflI RGATCY 2 cut(s) 156, 1189
MhlI GDGCHC 3 cut(s) 31, 378, 750
MluCI AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1254
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 2 cut(s) 754, 1248
Mph1103I ATGCAT 1 cut(s) 1200
MroXI GAANNNNTTC 1 cut(s) 1353
MseI TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1383, 1446
MslI CAYNNNNRTG 2 cut(s) 136, 1199
MspI CCGG 2 cut(s) 88, 351
MspR9I CCNGG 1 cut(s) 88
MunI CAATTG 1 cut(s) 1254
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 1 cut(s) 1109
NciI CCSGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 1200
NdeII GATC 5 cut(s) 156, 697, 919, 960, 1189
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 8 cut(s) 301, 737, 773, 844, 862, 1130, 1198, 1265
NlaIV GGNNCC 2 cut(s) 375, 747
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 392
NsbI TGCGCA 1 cut(s) 1260
NsiI ATGCAT 1 cut(s) 1200
NspI RCATGY 3 cut(s) 773, 862, 1265
PaeI GCATGC 1 cut(s) 1265
PaeR7I CTCGAG 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 1182
PcsI WCGNNNNNNNCGW 1 cut(s) 819
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 3 cut(s) 383, 1218, 1408
PkrI GCNGC 2 cut(s) 187, 1490
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PshBI ATTAAT 1 cut(s) 1043
PsiI TTATAA 3 cut(s) 522, 1281, 1416
PspFI CCCAGC 3 cut(s) 62, 773, 1159
PspN4I GGNNCC 2 cut(s) 375, 747
PspPI GGNCC 3 cut(s) 85, 187, 1157
PspXI VCTCGAGB 1 cut(s) 326
PsuI RGATCY 2 cut(s) 156, 1189
RsaI GTAC 3 cut(s) 368, 1097, 1508
RsaNI GTAC 3 cut(s) 367, 1096, 1507
RseI CAYNNNNRTG 2 cut(s) 136, 1199
SapI GCTCTTC 1 cut(s) 1182
SaqAI TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1383, 1446
SatI GCNGC 2 cut(s) 186, 1489
Sau3AI GATC 5 cut(s) 156, 697, 919, 960, 1189
Sau96I GGNCC 3 cut(s) 85, 187, 1157
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 3 cut(s) 31, 378, 750
SfaNI GCATC 2 cut(s) 412, 505
SfcI CTRYAG 1 cut(s) 1018
Sfr274I CTCGAG 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 136, 1199
SmlI CTYRAG 3 cut(s) 326, 341, 1402
SmoI CTYRAG 3 cut(s) 326, 341, 1402
SphI GCATGC 1 cut(s) 1265
Sse9I AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1254
SsiI CCGC 4 cut(s) 117, 185, 565, 1489
SspMI CTAG 5 cut(s) 150, 908, 950, 971, 1317
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 5 cut(s) 172, 608, 1345, 1423, 1495
TaiI ACGT 1 cut(s) 34
TaqI TCGA 3 cut(s) 327, 897, 1462
TaqII GACCGA 1 cut(s) 291
TasI AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1254
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 2 cut(s) 188, 1491
TfiI GAWTC 3 cut(s) 383, 1218, 1408
Tru1I TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1383, 1446
Tru9I TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1383, 1446
TscAI CASTG 4 cut(s) 177, 686, 1105, 1498
TseFI GTSAC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 6 cut(s) 545, 617, 628, 750, 857, 1343
TspRI CASTG 4 cut(s) 177, 686, 1105, 1498
VspI ATTAAT 1 cut(s) 1043
XagI CCTNNNNNAGG 1 cut(s) 974
XapI RAATTY 2 cut(s) 635, 830
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 3 cut(s) 773, 862, 1265
XhoI CTCGAG 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 5 cut(s) 150, 908, 950, 971, 1317
Zsp2I ATGCAT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.