MD06G1015700.v1.1

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001359
Physical Location & Seq
Reverse (-)
1964269 .. 1971138
6870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1015700.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGAGCAAATCACAAGGTTTCTCGTCGTCACGTCTCATCGTGAAACAAGGCATTAAGAAACCCAGCAAAGCCCCACTCCCCTTGGCCCGGAAAATCTCCACTCGGCCACACTTTCCCGCCACCCCTCAGCCACCGAAATGGTCGTTTTCTAGCAAGATCCCTTATTCAACAGTGTCATCCAGAGCGGCCCAAGACCTTCTGGCGGAGGTGGAGAGAGAAAAGCAGAGAGAACGAGAGCAGAGGAAGAGGGTGGGGCTGGACACCAAAGACATTGACCGAGAAGATGAGGAGGACTACATGGGCGTTATGCCATTGATCGAGAAGCTCGAGAAGGAGAAGCTCAAGGACACCGGAGACTTGAACTTGTACGAGGAGCCCACCGATTCCGATAGTGACGAAGACGATGAGAGGTTCACTCCTGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAGGAAGTTCACACGCCACGAGGAGTTGCTCAACAACTTCACCGAGGCTGAGACACTTGATGATGCTTATAAATGGATGAATAAAATTGACAAGTTTGAGCAAAAACATTTCCGCCTTCGCCCCGAATATAGGGTCATTGGTGAGTTGATGAACCGTCTCAAAGTAGCTGAGGGAAAGGACAAATTCATCCTCCAGCAGAAACTAAACAGGGCTATGAGATTGGTGCAGTGGAAGGAAGCCTACGATCCAAACAATCCTGCCAATTATGGAGTCATCCAACATGAGCAAGTGGAGCCCTCAGTTGATTTATTGGAACATGCTGGGTTTGAAAAACAAAAGCAAATTATACAAGGAGTTGACGACGACAACGAAGAAGAATTTAATGACATGAAGGAGAAAGATGACATACTGTTAGAGAAGCTTAATGCCATTGACAAAAAACTCGAAGAGAAGCTAGCAGAGTTAGATCATACATTCGGGAAGAAGGGTAAGGTTCTAGAGGAAGAGATCAGAGACCTAGCAGAGGAAAGAAATGATTTGACAGAGAAGAAGAGGAGACCTCTCTACAGGAAAGGGTTTGATGTTAAATTAATCAATGTGAACCGAACTTGTAAAGTAACAAAGGGGGGGCAAGTGGTCAAGTACACTGCCATATTAGCTTGTGGCAATTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGCCCAGCAGTCCCTATCGCTCTTCAGAAGGCATATGAGAAATGCTTTCAGAATCTCCACTACATTGAACGACACGAGGAGCATACAATTGCGCATGCAATACAAACAAGTTATAAAAAAACAAAGGTGTATCTCTGGCCTGCACCCACTAGGACAGGTATGAAAGCAGGAAGAACTGTTGAAACCATTCTAAACTTAGCTGGTTTCAAAAATGTTAAGTCTAAGGTTGTAGGCTCAAGGAATCCTTATAACACAGTCAAGGCTCTCTTCAAATCCCTTAATGCGATTGAAACTCGAAAGGATGTTCAAGATAAGTTTGGCCGCACTGTTGTTGAGAAGTACCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

502

Amino Acids

58.11

Weight (kDa)

8.96

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 344 - 408 1e-17 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 423 - 489 5.1e-21 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 522, 1269, 1404
Acc16I TGCGCA 1 cut(s) 1248
AccBSI CCGCTC 1 cut(s) 185
AciI CCGC 5 cut(s) 117, 185, 203, 565, 1477
AclWI GGATC 2 cut(s) 151, 692
AcoI YGGCCR 2 cut(s) 104, 1474
AcsI RAATTY 2 cut(s) 635, 830
AcuI CTGAAG 1 cut(s) 1163
AfaI GTAC 3 cut(s) 368, 1097, 1496
AfiI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
AgsI TTSAA 9 cut(s) 168, 361, 782, 1223, 1337, 1363, 1426, 1445, 1463
AjiI CACGTC 1 cut(s) 32
AluBI AGCT 7 cut(s) 325, 340, 620, 874, 907, 1112, 1355
AluI AGCT 7 cut(s) 325, 340, 620, 874, 907, 1112, 1355
Alw26I GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
AlwI GGATC 2 cut(s) 151, 692
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 6 cut(s) 84, 104, 186, 1156, 1292, 1474
ApoI RAATTY 2 cut(s) 635, 830
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
AseI ATTAAT 1 cut(s) 1043
Asp700I GAANNNNTTC 1 cut(s) 1341
AspLEI GCGC 1 cut(s) 1249
AspS9I GGNCC 3 cut(s) 85, 187, 1157
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 484, 605
AsuNHI GCTAGC 1 cut(s) 907
AvaI CYCGRG 1 cut(s) 326
BanII GRGCYC 2 cut(s) 378, 750
BarI GAAGNNNNNNTAC 2 cut(s) 677, 709
BauI CACGAG 2 cut(s) 470, 1229
BbsI GAAGAC 1 cut(s) 405
BbvCI CCTCAGC 2 cut(s) 126, 621
BceAI ACGGC 1 cut(s) 410
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BfaI CTAG 5 cut(s) 150, 908, 950, 971, 1305
BfmI CTRYAG 1 cut(s) 1018
BisI GCNGC 2 cut(s) 186, 1477
BlsI GCNGC 2 cut(s) 187, 1478
Bme1390I CCNGG 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 85, 187, 1157
BmiI GGNNCC 2 cut(s) 375, 747
BmrFI CCNGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 412, 505
BmtI GCTAGC 1 cut(s) 911
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 4 cut(s) 400, 432, 997, 1029
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 2 cut(s) 126, 621
BpuEI CTTGAG 2 cut(s) 326, 1375
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 2 cut(s) 960, 1003
BsaJI CCNNGG 2 cut(s) 81, 495
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BseDI CCNNGG 2 cut(s) 81, 495
BseGI GGATG 5 cut(s) 176, 534, 639, 726, 1462
BseLI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BseMII CTCAG 4 cut(s) 140, 492, 612, 765
BseRI GAGGAG 5 cut(s) 302, 386, 488, 1021, 1247
BseYI CCCAGC 3 cut(s) 62, 773, 1159
BsgI GTGCAG 2 cut(s) 698, 1281
BshFI GGCC 6 cut(s) 86, 106, 188, 1158, 1294, 1476
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 2 cut(s) 88, 351
BslFI GGGAC 1 cut(s) 1151
BslI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BsmAI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BsmBI CGTCTC 2 cut(s) 38, 614
BsmFI GGGAC 1 cut(s) 1151
BsnI GGCC 6 cut(s) 86, 106, 188, 1158, 1294, 1476
Bso31I GGTCTC 2 cut(s) 960, 1003
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 378, 750
Bsp143I GATC 5 cut(s) 156, 315, 697, 919, 960
BspACI CCGC 5 cut(s) 117, 185, 203, 565, 1477
BspANI GGCC 6 cut(s) 86, 106, 188, 1158, 1294, 1476
BspCNI CTCAG 4 cut(s) 139, 493, 613, 764
BspLI GGNNCC 2 cut(s) 375, 747
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 2 cut(s) 151, 692
BspQI GCTCTTC 1 cut(s) 1182
BspTNI GGTCTC 2 cut(s) 960, 1003
BsrBI CCGCTC 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 81, 495
BssMI GATC 5 cut(s) 156, 315, 697, 919, 960
BssSI CACGAG 2 cut(s) 470, 1229
BssT1I CCWWGG 1 cut(s) 81
Bst2BI CACGAG 2 cut(s) 470, 1229
Bst4CI ACNGT 6 cut(s) 172, 608, 864, 1333, 1411, 1483
Bst6I CTCTTC 7 cut(s) 239, 428, 894, 951, 998, 1182, 1427
BstC8I GCNNGC 3 cut(s) 909, 1251, 1296
BstDEI CTNAG 6 cut(s) 126, 501, 621, 751, 1351, 1377
BstENI CCTNNNNNAGG 1 cut(s) 974
BstF5I GGATG 5 cut(s) 176, 534, 639, 726, 1462
BstHHI GCGC 1 cut(s) 1249
BstKTI GATC 5 cut(s) 159, 318, 700, 922, 963
BstMAI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BstMBI GATC 5 cut(s) 156, 315, 697, 919, 960
BstMWI GCNNNNNNNGC 2 cut(s) 745, 1109
BstNSI RCATGY 2 cut(s) 773, 1253
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 1018
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 138
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 6 cut(s) 86, 106, 188, 1158, 1294, 1476
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 5 cut(s) 176, 534, 639, 726, 1462
BtsI GCAGTG 2 cut(s) 686, 1098
BtsIMutI CAGTG 4 cut(s) 177, 686, 1098, 1479
Cac8I GCNNGC 3 cut(s) 909, 1251, 1296
CfoI GCGC 1 cut(s) 1249
Cfr13I GGNCC 3 cut(s) 85, 187, 1157
Csp6I GTAC 3 cut(s) 367, 1096, 1495
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 6 cut(s) 298, 734, 770, 841, 1127, 1250
CviQI GTAC 3 cut(s) 367, 1096, 1495
DdeI CTNAG 6 cut(s) 126, 501, 621, 751, 1351, 1377
DpnI GATC 5 cut(s) 158, 317, 699, 921, 962
DpnII GATC 5 cut(s) 156, 315, 697, 919, 960
EaeI YGGCCR 2 cut(s) 104, 1474
Eam1104I CTCTTC 7 cut(s) 239, 428, 894, 951, 998, 1182, 1427
EarI CTCTTC 7 cut(s) 239, 428, 894, 951, 998, 1182, 1427
EciI GGCGGA 2 cut(s) 218, 554
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 378, 750
Eco31I GGTCTC 2 cut(s) 960, 1003
Eco57I CTGAAG 1 cut(s) 1163
Eco88I CYCGRG 1 cut(s) 326
EcoNI CCTNNNNNAGG 1 cut(s) 974
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 2 cut(s) 378, 750
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 2 cut(s) 38, 614
FaeI CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
FalI AAGNNNNNCTT 4 cut(s) 1384, 1416, 1406, 1438
FaqI GGGAC 1 cut(s) 1151
FatI CATG 6 cut(s) 297, 733, 769, 840, 1126, 1249
FauI CCCGC 1 cut(s) 124
FauNDI CATATG 1 cut(s) 1189
Fnu4HI GCNGC 2 cut(s) 186, 1477
FokI GGATG 5 cut(s) 163, 541, 626, 713, 1469
FriOI GRGCYC 2 cut(s) 378, 750
Fsp4HI GCNGC 2 cut(s) 186, 1477
FspBI CTAG 5 cut(s) 150, 908, 950, 971, 1305
FspI TGCGCA 1 cut(s) 1248
GlaI GCGC 1 cut(s) 1248
GluI GCNGC 2 cut(s) 186, 1477
GsaI CCCAGC 3 cut(s) 66, 777, 1163
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 6 cut(s) 86, 106, 188, 1158, 1294, 1476
HapII CCGG 2 cut(s) 88, 351
HhaI GCGC 1 cut(s) 1249
Hin1II CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
Hin6I GCGC 1 cut(s) 1247
HinP1I GCGC 1 cut(s) 1247
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 4 cut(s) 383, 723, 1207, 1396
HpaII CCGG 2 cut(s) 88, 351
HphI GGTGA 2 cut(s) 484, 605
Hpy166II GTNNAC 5 cut(s) 414, 462, 811, 1054, 1098
Hpy188I TCNGA 5 cut(s) 388, 453, 965, 1182, 1206
Hpy188III TCNNGA 9 cut(s) 40, 180, 319, 328, 419, 430, 931, 950, 1463
Hpy8I GTNNAC 5 cut(s) 414, 462, 811, 1054, 1098
Hpy99I CGWCG 2 cut(s) 28, 818
HpyAV CCTTC 7 cut(s) 206, 325, 578, 679, 838, 931, 1177
HpyCH4III ACNGT 6 cut(s) 172, 608, 864, 1333, 1411, 1483
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 679, 1253, 1298
HpyF10VI GCNNNNNNNGC 2 cut(s) 745, 1109
HpyF3I CTNAG 6 cut(s) 126, 501, 621, 751, 1351, 1377
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
HspAI GCGC 1 cut(s) 1247
Kzo9I GATC 5 cut(s) 156, 315, 697, 919, 960
LguI GCTCTTC 1 cut(s) 1182
LmnI GCTCC 3 cut(s) 373, 745, 1234
LweI GCATC 2 cut(s) 412, 505
MaeI CTAG 5 cut(s) 150, 908, 950, 971, 1305
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 3 cut(s) 27, 392, 1069
MalI GATC 5 cut(s) 158, 317, 699, 921, 962
MbiI CCGCTC 1 cut(s) 185
MboI GATC 5 cut(s) 156, 315, 697, 919, 960
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 378, 750
MluCI AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1242
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 1 cut(s) 754
MroXI GAANNNNTTC 1 cut(s) 1341
MseI TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1371, 1434
MslI CAYNNNNRTG 1 cut(s) 136
MspI CCGG 2 cut(s) 88, 351
MspR9I CCNGG 1 cut(s) 88
MunI CAATTG 1 cut(s) 1242
MwoI GCNNNNNNNGC 2 cut(s) 745, 1109
NciI CCSGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 1189
NdeII GATC 5 cut(s) 156, 315, 697, 919, 960
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
NlaIV GGNNCC 2 cut(s) 375, 747
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 27, 392
NsbI TGCGCA 1 cut(s) 1248
NspI RCATGY 2 cut(s) 773, 1253
PaeI GCATGC 1 cut(s) 1253
PaeR7I CTCGAG 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 1182
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 819
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 3 cut(s) 383, 1207, 1396
PkrI GCNGC 2 cut(s) 187, 1478
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PshBI ATTAAT 1 cut(s) 1043
PsiI TTATAA 3 cut(s) 522, 1269, 1404
PspFI CCCAGC 3 cut(s) 62, 773, 1159
PspN4I GGNNCC 2 cut(s) 375, 747
PspPI GGNCC 3 cut(s) 85, 187, 1157
PsuI RGATCY 1 cut(s) 156
RsaI GTAC 3 cut(s) 368, 1097, 1496
RsaNI GTAC 3 cut(s) 367, 1096, 1495
RseI CAYNNNNRTG 1 cut(s) 136
SapI GCTCTTC 1 cut(s) 1182
SaqAI TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1371, 1434
SatI GCNGC 2 cut(s) 186, 1477
Sau3AI GATC 5 cut(s) 156, 315, 697, 919, 960
Sau96I GGNCC 3 cut(s) 85, 187, 1157
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 2 cut(s) 378, 750
SfaNI GCATC 2 cut(s) 412, 505
SfcI CTRYAG 1 cut(s) 1018
Sfr274I CTCGAG 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 136
SmlI CTYRAG 3 cut(s) 326, 341, 1390
SmoI CTYRAG 3 cut(s) 326, 341, 1390
SphI GCATGC 1 cut(s) 1253
Sse9I AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1242
SsiI CCGC 5 cut(s) 117, 185, 203, 565, 1477
SspMI CTAG 5 cut(s) 150, 908, 950, 971, 1305
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 6 cut(s) 172, 608, 864, 1333, 1411, 1483
TaiI ACGT 1 cut(s) 34
TaqI TCGA 4 cut(s) 318, 327, 897, 1450
TaqII GACCGA 1 cut(s) 291
TasI AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1242
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 2 cut(s) 188, 1479
TfiI GAWTC 3 cut(s) 383, 1207, 1396
Tru1I TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1371, 1434
Tru9I TTAA 7 cut(s) 54, 834, 876, 1038, 1043, 1371, 1434
TscAI CASTG 4 cut(s) 177, 686, 1105, 1486
TseFI GTSAC 2 cut(s) 27, 392
Tsp45I GTSAC 2 cut(s) 27, 392
TspDTI ATGAA 5 cut(s) 545, 617, 628, 857, 1331
TspRI CASTG 4 cut(s) 177, 686, 1105, 1486
VspI ATTAAT 1 cut(s) 1043
XagI CCTNNNNNAGG 1 cut(s) 974
XapI RAATTY 2 cut(s) 635, 830
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 2 cut(s) 773, 1253
XhoI CTCGAG 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 5 cut(s) 150, 908, 950, 971, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.