RLG00000023329

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001359
Physical Location & Seq
Forward (+)
22050529 .. 22053783
3255 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023329

Sequence Viewer

Length: 693 bp
ATGCGTGTCACACCATTGATAGAGAAGCTTGAGAAGGAGAAGCTCAAGACCACTGGGGATCTCAATTCCTATGAGGAGCCCACCAATTCTGATAGTGACGATGACGACGAGCGGTTCAGTCCCAATGCCTTCAAGAAGAGGGTTGATGAGTTTCAGAGGAAGTTCACACGACATGAGGAATTGCTCAAGAATTTCACACAGGGATTCGATGTTAGATTAATTGATGTGAACCGGACTTGTAAAGTTACAAAGGGAGGGCAAGTGGTCAAATACACTGCTATATTAGTTTGTGGGAACTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGTCCAGCCGTCCCTATTGCCCTCCAGAAGGCATATGAGAAATGCTTTCAGAATCTCCATCACGTAGAACGACATGAGGAGCATGCAATTGTGCATGCAATACAAACTACATATAAAAAAACAAAGGTAATAGCCTCATCTTGCATTAGTGTATCTTTGGCCTGCACCACTAGGACGGGTATGAAAGCGGGAAGAACTGTCGAAACCATTCTAAACTTGGCTGGTTTCAATAATGTTGTTGGTTCAAGGAGTCCTCATAATACAGTGAAGGCTGTTTTCAAAGCCCTTAATGTGATTGAAACTTCCAAGGATGTTCAAGAAAATTTTGGGCGCACTGTTGTTGAGAAGTACCTTCTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

231

Amino Acids

25.83

Weight (kDa)

9.18

Isoelectric Point (pI)

30.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 69 - 133 7.5e-19 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 168 - 217 5.7e-10 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 112
AciI CCGC 2 cut(s) 112, 518
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 2 cut(s) 190, 652
AfaI GTAC 1 cut(s) 680
AfiI CCNNNNNNNGG 1 cut(s) 358
AgsI TTSAA 6 cut(s) 133, 559, 576, 610, 629, 647
AluBI AGCT 2 cut(s) 28, 43
AluI AGCT 2 cut(s) 28, 43
AlwI GGATC 1 cut(s) 66
AoxI GGCC 1 cut(s) 489
ApoI RAATTY 2 cut(s) 190, 652
AseI ATTAAT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 537
AspLEI GCGC 1 cut(s) 663
AspS9I GGNCC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 81
BccI CCATC 1 cut(s) 396
BceAI ACGGC 1 cut(s) 323
BfaI CTAG 1 cut(s) 501
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 78
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 338
BpuEI CTTGAG 3 cut(s) 29, 50, 170
BsaAI YACGTR 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 636
BsaWI WCCGGW 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse1I ACTGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 1 cut(s) 646
BseLI CCNNNNNNNGG 1 cut(s) 358
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 89, 422
BsgI GTGCAG 1 cut(s) 478
BshFI GGCC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 232
BslFI GGGAC 2 cut(s) 105, 326
BslI CCNNNNNNNGG 1 cut(s) 358
BsmFI GGGAC 2 cut(s) 105, 326
BsnI GGCC 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 58
BspACI CCGC 2 cut(s) 112, 518
BspANI GGCC 1 cut(s) 491
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 66
BsrBI CCGCTC 1 cut(s) 112
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 636
BssMI GATC 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 636
Bst4CI ACNGT 3 cut(s) 529, 595, 667
Bst6I CTCTTC 1 cut(s) 131
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 414, 426, 493
BstENI CCTNNNNNAGG 1 cut(s) 356
BstF5I GGATG 1 cut(s) 646
BstHHI GCGC 1 cut(s) 663
BstKTI GATC 1 cut(s) 61
BstMBI GATC 1 cut(s) 58
BstNSI RCATGY 2 cut(s) 416, 428
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 1 cut(s) 491
BtsCI GGATG 1 cut(s) 646
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 4 cut(s) 51, 273, 600, 663
Cac8I GCNNGC 3 cut(s) 414, 426, 493
CfoI GCGC 1 cut(s) 663
Cfr13I GGNCC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 679
CviAII CATG 5 cut(s) 173, 302, 404, 413, 425
CviJI RGCY 9 cut(s) 28, 43, 79, 338, 464, 491, 551, 602, 614
CviKI_1 RGCY 9 cut(s) 28, 43, 79, 338, 464, 491, 551, 602, 614
CviQI GTAC 1 cut(s) 679
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 636
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 636
FaeI CATG 5 cut(s) 176, 305, 407, 416, 428
FaqI GGGAC 2 cut(s) 105, 326
FatI CATG 5 cut(s) 172, 301, 403, 412, 424
FauI CCCGC 1 cut(s) 511
FauNDI CATATG 1 cut(s) 364
FokI GGATG 1 cut(s) 653
FriOI GRGCYC 1 cut(s) 81
FspBI CTAG 1 cut(s) 501
GlaI GCGC 1 cut(s) 662
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 1 cut(s) 491
HapII CCGG 1 cut(s) 232
HhaI GCGC 1 cut(s) 663
Hin1II CATG 5 cut(s) 176, 305, 407, 416, 428
Hin6I GCGC 1 cut(s) 661
HinP1I GCGC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 3 cut(s) 204, 382, 580
HpaII CCGG 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 165, 229
Hpy188I TCNGA 3 cut(s) 91, 156, 381
Hpy188III TCNNGA 5 cut(s) 46, 133, 187, 355, 647
Hpy8I GTNNAC 2 cut(s) 165, 229
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 5 cut(s) 28, 139, 352, 592, 692
HpyCH4III ACNGT 3 cut(s) 529, 595, 667
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 5 cut(s) 416, 424, 428, 474, 495
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 5 cut(s) 176, 305, 407, 416, 428
HspAI GCGC 1 cut(s) 661
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 2 cut(s) 76, 409
LpnPI CCDG 7 cut(s) 39, 185, 245, 348, 368, 505, 537
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 7, 95, 244
MalI GATC 1 cut(s) 60
MbiI CCGCTC 1 cut(s) 112
MboI GATC 1 cut(s) 58
MboII GAAGA 2 cut(s) 148, 534
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 7 cut(s) 64, 85, 179, 190, 219, 417, 652
MlyI GAGTC 1 cut(s) 589
MnlI CCTC 9 cut(s) 67, 132, 150, 169, 248, 362, 400, 475, 594
MroXI GAANNNNTTC 1 cut(s) 537
MseI TTAA 2 cut(s) 218, 618
MspI CCGG 1 cut(s) 232
MunI CAATTG 1 cut(s) 417
NdeI CATATG 1 cut(s) 364
NdeII GATC 1 cut(s) 58
NlaIII CATG 5 cut(s) 176, 305, 407, 416, 428
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 2 cut(s) 7, 95
NspI RCATGY 2 cut(s) 416, 428
PaeI GCATGC 2 cut(s) 416, 428
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 537
PfeI GAWTC 2 cut(s) 204, 382
PleI GAGTC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 588
Ppu21I YACGTR 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 332
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
SaqAI TTAA 2 cut(s) 218, 618
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 1 cut(s) 589
SduI GDGCHC 1 cut(s) 81
SetI ASST 6 cut(s) 30, 45, 334, 396, 459, 684
SinI GGWCC 1 cut(s) 332
SmlI CTYRAG 3 cut(s) 29, 44, 185
SmoI CTYRAG 3 cut(s) 29, 44, 185
SphI GCATGC 2 cut(s) 416, 428
Sse9I AATT 7 cut(s) 64, 85, 179, 190, 219, 417, 652
SsiI CCGC 2 cut(s) 112, 518
SspMI CTAG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 636
TaaI ACNGT 3 cut(s) 529, 595, 667
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 207, 531
TasI AATT 7 cut(s) 64, 85, 179, 190, 219, 417, 652
TfiI GAWTC 2 cut(s) 204, 382
Tru1I TTAA 2 cut(s) 218, 618
Tru9I TTAA 2 cut(s) 218, 618
TscAI CASTG 4 cut(s) 58, 280, 600, 670
TseFI GTSAC 2 cut(s) 7, 95
Tsp45I GTSAC 2 cut(s) 7, 95
TspDTI ATGAA 1 cut(s) 527
TspRI CASTG 4 cut(s) 58, 280, 600, 670
VpaK11BI GGWCC 1 cut(s) 332
VspI ATTAAT 1 cut(s) 218
XagI CCTNNNNNAGG 1 cut(s) 356
XapI RAATTY 2 cut(s) 190, 652
XceI RCATGY 2 cut(s) 416, 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XmnI GAANNNNTTC 1 cut(s) 537
XspI CTAG 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.