MD07G1311200.v1.1

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001359
Physical Location & Seq
Reverse (-)
36626496 .. 36630501
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1311200.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGGTCGTTTTCCAGCAAGATCCCTTATTCAACAGTGTCATCCAGAGCGGCGCAAGACCTTCTGGTGAGGTGGAGAGAGAAAAGCAGAGAGAACGAGAGCAGAGGAAGAGGGCGGGCCTGGACACCAAAGACATTGACCGAGAAGATAAGGAGGACTACATGGGCGTTATGCCATTGATTGAGAAGCTCGAGCAGGAGAAGCTCAAGGACATCGGAGACTTGAACTTGTACGAGGAGCCCACCGATTCCGATAGCGACGAAGACGATGAAAGGTTCACTCCCGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAGGAAGTTCACGCGCCACGAGGAGTTGCTCAACAACTTCATCGAGGCTGAGACACTTGATGATGCTTATAAATGGATGAATAAAATTGACAAGTTTGAGCAAAAACATTTCCGCCTTCGCCCCGAATATAGGGTCATTGGTGAGTTGATGAACCGTCTCAAAGTAGTCGAGGGAAATGACAAATTCATCCTCCAGCAGAAACTAAACAGGGCTATGAGATTGGTGCAGTGGAAGGAAGCCTACGATCCCAACAATCCTGCCAATTATGGAGTCATCCAACATGAACAAGTGGAGCCCTCAGTTGATTTATTGGAACATGCTGGGTTTGAAAAAGAAAAGCAAATTATACAAGGAGTTGATGACGACGACGGGGAAGAATTCAATGACATACTGTTAGAGAAGCTTAATGCCATTGACAAAAAACTCGAAGAGAAGCTAGCAGAGTTAGATCATACATTCGGGAAGAAGGGTAAGGTTCTAGAGGAAGAGATCAGAGACCTAGCAGAGGAAAGAAATGATTTGATAGAGAAGAAGAGGAGACCTCTCTACAGGAAAGGGTTTGATGTTATATTAATCAATGTGAACCGAACCTGTAAAGTAACAAAGGCACTCCACCTTGCGGTTTATTTTCTGTGGGGGGCAAGTGGTCAAGTACACTGCCATATTAGCTTGTGGCAATTATCATGGTGTTGCTGCTCCCAAGTCTTGAACCCGAGACCTACCGCTCATGGGCGAAGGCACTTGCCATCGCACCAAGTGCGACCTCTTCACCAAGCATATGAGAAGTCCTTTCAGAATCTCCACTACATTGAACGACACAAGGAGCATACAATTGCGCATGCAATACAAACAAGTGATAAAAAAACAAAGGTGTATCTCTGGCCTGCACCCACTAGGACGGGTATGAAAGCAGGAAGAACTGTTGAAACCATTCTAAACTTAGCTGGTTTCAAAAATGTTAAGTCTAAGGTATTGACTTTGAAAGAAATCCATTTTAAGAGGGGTGGTTCCTGCAATTTGGTTTGTTGCTCTAACCTCCACACTTGTCAGGTTTTAGGCTCAAGGAATCCTTATAACACAGTCAAGGCTCTCTTCAAATCCCTTAATGCGGTCAATAGTCACTTTAACCGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

484

Amino Acids

56.48

Weight (kDa)

7.3

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5_C PF03719 392 - 430 5.7e-09 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 384, 1389
Acc16I TGCGCA 1 cut(s) 1152
AccBSI CCGCTC 2 cut(s) 48, 1040
AccII CGCG 1 cut(s) 328
AciI CCGC 6 cut(s) 48, 113, 427, 935, 1038, 1424
AclWI GGATC 2 cut(s) 14, 554
AcsI RAATTY 2 cut(s) 497, 692
AdeI CACNNNGTG 1 cut(s) 1072
AfaI GTAC 2 cut(s) 230, 969
AfiI CCNNNNNNNGG 5 cut(s) 444, 820, 934, 1044, 1423
AgeI ACCGGT 1 cut(s) 1443
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 6 cut(s) 187, 202, 718, 751, 984, 1259
AluI AGCT 6 cut(s) 187, 202, 718, 751, 984, 1259
Alw26I GTCTC 6 cut(s) 210, 359, 476, 804, 847, 1024
AlwI GGATC 2 cut(s) 14, 554
Ama87I CYCGRG 2 cut(s) 188, 1027
AoxI GGCC 2 cut(s) 115, 1196
ApeKI GCWGC 1 cut(s) 1008
ApoI RAATTY 2 cut(s) 497, 692
ArsI GACNNNNNNTTYG 2 cut(s) 432, 464
AseI ATTAAT 1 cut(s) 887
AsiGI ACCGGT 1 cut(s) 1443
Asp700I GAANNNNTTC 1 cut(s) 1245
AspLEI GCGC 3 cut(s) 53, 330, 1153
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 3 cut(s) 77, 467, 1076
AsuNHI GCTAGC 1 cut(s) 751
AvaI CYCGRG 2 cut(s) 188, 1027
BanII GRGCYC 2 cut(s) 240, 612
BarI GAAGNNNNNNTAC 2 cut(s) 539, 571
BauI CACGAG 1 cut(s) 332
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 995
BccI CCATC 1 cut(s) 1069
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 1 cut(s) 119
BcoDI GTCTC 6 cut(s) 210, 359, 476, 804, 847, 1024
BfaI CTAG 4 cut(s) 752, 794, 815, 1209
BfmI CTRYAG 1 cut(s) 862
BisI GCNGC 2 cut(s) 49, 1009
BlsI GCNGC 2 cut(s) 50, 1010
Bme1390I CCNGG 1 cut(s) 119
BmeT110I CYCGRG 2 cut(s) 188, 1027
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 3 cut(s) 237, 609, 1324
BmrFI CCNGG 1 cut(s) 119
BmsI GCATC 2 cut(s) 274, 367
BmtI GCTAGC 1 cut(s) 755
BpiI GAAGAC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpmI CTGGAG 1 cut(s) 491
BpuEI CTTGAG 2 cut(s) 188, 1360
BsaI GGTCTC 3 cut(s) 804, 847, 1024
BsaWI WCCGGW 1 cut(s) 1443
Bsc4I CCNNNNNNNGG 5 cut(s) 444, 820, 934, 1044, 1423
Bse118I RCCGGY 1 cut(s) 1443
BseBI CCWGG 1 cut(s) 119
BseGI GGATG 4 cut(s) 39, 396, 501, 588
BseLI CCNNNNNNNGG 5 cut(s) 444, 820, 934, 1044, 1423
BseMII CTCAG 2 cut(s) 354, 627
BseRI GAGGAG 3 cut(s) 248, 350, 865
BseXI GCAGC 1 cut(s) 995
BseYI CCCAGC 1 cut(s) 635
BsgI GTGCAG 2 cut(s) 560, 1185
Bsh1236I CGCG 1 cut(s) 328
BshFI GGCC 2 cut(s) 117, 1198
BshTI ACCGGT 1 cut(s) 1443
BsiHKCI CYCGRG 2 cut(s) 188, 1027
BsiSI CCGG 1 cut(s) 1444
BslI CCNNNNNNNGG 5 cut(s) 444, 820, 934, 1044, 1423
BsmAI GTCTC 6 cut(s) 210, 359, 476, 804, 847, 1024
BsmBI CGTCTC 1 cut(s) 476
BsnI GGCC 2 cut(s) 117, 1198
Bso31I GGTCTC 3 cut(s) 804, 847, 1024
BsoBI CYCGRG 2 cut(s) 188, 1027
Bsp1286I GDGCHC 2 cut(s) 240, 612
Bsp143I GATC 4 cut(s) 19, 559, 763, 804
BspACI CCGC 6 cut(s) 48, 113, 427, 935, 1038, 1424
BspANI GGCC 2 cut(s) 117, 1198
BspCNI CTCAG 2 cut(s) 355, 626
BspFNI CGCG 1 cut(s) 328
BspLI GGNNCC 3 cut(s) 237, 609, 1324
BspOI GCTAGC 1 cut(s) 755
BspPI GGATC 2 cut(s) 14, 554
BspTNI GGTCTC 3 cut(s) 804, 847, 1024
BsrBI CCGCTC 2 cut(s) 48, 1040
BsrFI RCCGGY 1 cut(s) 1443
BssAI RCCGGY 1 cut(s) 1443
BssMI GATC 4 cut(s) 19, 559, 763, 804
BssSI CACGAG 1 cut(s) 332
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 5 cut(s) 35, 470, 708, 1237, 1396
Bst6I CTCTTC 7 cut(s) 101, 290, 738, 795, 842, 1086, 1412
BstAPI GCANNNNNTGC 1 cut(s) 1072
BstC8I GCNNGC 4 cut(s) 115, 753, 1155, 1200
BstDEI CTNAG 4 cut(s) 363, 613, 1255, 1281
BstENI CCTNNNNNAGG 1 cut(s) 818
BstF5I GGATG 4 cut(s) 39, 396, 501, 588
BstFNI CGCG 1 cut(s) 328
BstHHI GCGC 3 cut(s) 53, 330, 1153
BstKTI GATC 4 cut(s) 22, 562, 766, 807
BstMAI GTCTC 6 cut(s) 210, 359, 476, 804, 847, 1024
BstMBI GATC 4 cut(s) 19, 559, 763, 804
BstMWI GCNNNNNNNGC 3 cut(s) 199, 981, 1072
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 2 cut(s) 635, 1157
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 1 cut(s) 862
BstUI CGCG 1 cut(s) 328
BstV1I GCAGC 1 cut(s) 995
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuRI GGCC 2 cut(s) 117, 1198
BtgZI GCGATG 1 cut(s) 1047
BtsCI GGATG 4 cut(s) 39, 396, 501, 588
BtsI GCAGTG 2 cut(s) 548, 970
BtsIMutI CAGTG 3 cut(s) 40, 548, 970
Cac8I GCNNGC 4 cut(s) 115, 753, 1155, 1200
CfoI GCGC 3 cut(s) 53, 330, 1153
Cfr10I RCCGGY 1 cut(s) 1443
Cfr13I GGNCC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 229, 968
CspAI ACCGGT 1 cut(s) 1443
CviAII CATG 6 cut(s) 160, 596, 632, 999, 1043, 1154
CviQI GTAC 2 cut(s) 229, 968
DdeI CTNAG 4 cut(s) 363, 613, 1255, 1281
DpnI GATC 4 cut(s) 21, 561, 765, 806
DpnII GATC 4 cut(s) 19, 559, 763, 804
DraIII CACNNNGTG 1 cut(s) 1072
Eam1104I CTCTTC 7 cut(s) 101, 290, 738, 795, 842, 1086, 1412
EarI CTCTTC 7 cut(s) 101, 290, 738, 795, 842, 1086, 1412
EciI GGCGGA 1 cut(s) 416
Eco24I GRGCYC 2 cut(s) 240, 612
Eco31I GGTCTC 3 cut(s) 804, 847, 1024
Eco88I CYCGRG 2 cut(s) 188, 1027
EcoNI CCTNNNNNAGG 1 cut(s) 818
EcoRI GAATTC 1 cut(s) 692
EcoRII CCWGG 1 cut(s) 117
EcoT38I GRGCYC 2 cut(s) 240, 612
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 6 cut(s) 163, 599, 635, 1002, 1046, 1157
FalI AAGNNNNNCTT 4 cut(s) 1369, 1401, 1391, 1423
FatI CATG 6 cut(s) 159, 595, 631, 998, 1042, 1153
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 1093
Fnu4HI GCNGC 2 cut(s) 49, 1009
FokI GGATG 4 cut(s) 26, 403, 488, 575
FriOI GRGCYC 2 cut(s) 240, 612
Fsp4HI GCNGC 2 cut(s) 49, 1009
FspBI CTAG 4 cut(s) 752, 794, 815, 1209
FspI TGCGCA 1 cut(s) 1152
GlaI GCGC 3 cut(s) 52, 329, 1152
GluI GCNGC 2 cut(s) 49, 1009
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 1 cut(s) 491
HaeIII GGCC 2 cut(s) 117, 1198
HapII CCGG 1 cut(s) 1444
HhaI GCGC 3 cut(s) 53, 330, 1153
Hin1II CATG 6 cut(s) 163, 599, 635, 1002, 1046, 1157
Hin6I GCGC 3 cut(s) 51, 328, 1151
HinP1I GCGC 3 cut(s) 51, 328, 1151
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 4 cut(s) 245, 585, 1111, 1381
HpaII CCGG 1 cut(s) 1444
HphI GGTGA 3 cut(s) 77, 467, 1076
Hpy166II GTNNAC 4 cut(s) 276, 324, 898, 970
Hpy188I TCNGA 5 cut(s) 215, 250, 315, 809, 1110
Hpy188III TCNNGA 6 cut(s) 43, 281, 292, 775, 794, 1021
Hpy8I GTNNAC 4 cut(s) 276, 324, 898, 970
Hpy99I CGWCG 3 cut(s) 260, 683, 686
HpyAV CCTTC 5 cut(s) 69, 440, 541, 775, 1044
HpyCH4III ACNGT 5 cut(s) 35, 470, 708, 1237, 1396
HpyCH4V TGCA 4 cut(s) 541, 1157, 1202, 1329
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 981, 1072
HpyF3I CTNAG 4 cut(s) 363, 613, 1255, 1281
Hsp92II CATG 6 cut(s) 163, 599, 635, 1002, 1046, 1157
HspAI GCGC 3 cut(s) 51, 328, 1151
Kzo9I GATC 4 cut(s) 19, 559, 763, 804
LmnI GCTCC 4 cut(s) 235, 607, 1016, 1138
Lsp1109I GCAGC 1 cut(s) 995
LweI GCATC 2 cut(s) 274, 367
MaeI CTAG 4 cut(s) 752, 794, 815, 1209
MaeIII GTNAC 2 cut(s) 913, 1433
MalI GATC 4 cut(s) 21, 561, 765, 806
MbiI CCGCTC 2 cut(s) 48, 1040
MboI GATC 4 cut(s) 19, 559, 763, 804
MfeI CAATTG 1 cut(s) 1146
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 2 cut(s) 240, 612
MluCI AATT 8 cut(s) 399, 497, 577, 657, 692, 992, 1146, 1330
MlyI GAGTC 1 cut(s) 594
MmeI TCCRAC 1 cut(s) 616
MroXI GAANNNNTTC 1 cut(s) 1245
MseI TTAA 6 cut(s) 720, 887, 1275, 1311, 1419, 1440
MspI CCGG 1 cut(s) 1444
MspR9I CCNGG 1 cut(s) 119
MunI CAATTG 1 cut(s) 1146
MvaI CCWGG 1 cut(s) 119
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 3 cut(s) 199, 981, 1072
NdeI CATATG 1 cut(s) 1093
NdeII GATC 4 cut(s) 19, 559, 763, 804
NheI GCTAGC 1 cut(s) 751
NlaIII CATG 6 cut(s) 163, 599, 635, 1002, 1046, 1157
NlaIV GGNNCC 3 cut(s) 237, 609, 1324
NmuCI GTSAC 1 cut(s) 1433
NsbI TGCGCA 1 cut(s) 1152
NspI RCATGY 2 cut(s) 635, 1157
PaeI GCATGC 1 cut(s) 1157
PaeR7I CTCGAG 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 3 cut(s) 245, 1111, 1381
PinAI ACCGGT 1 cut(s) 1443
PkrI GCNGC 2 cut(s) 50, 1010
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
PshBI ATTAAT 1 cut(s) 887
PsiI TTATAA 2 cut(s) 384, 1389
Psp6I CCWGG 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 3 cut(s) 237, 609, 1324
PspPI GGNCC 1 cut(s) 115
PspXI VCTCGAGB 1 cut(s) 188
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 230, 969
RsaNI GTAC 2 cut(s) 229, 968
SaqAI TTAA 6 cut(s) 720, 887, 1275, 1311, 1419, 1440
SatI GCNGC 2 cut(s) 49, 1009
Sau3AI GATC 4 cut(s) 19, 559, 763, 804
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 2 cut(s) 240, 612
SfaNI GCATC 2 cut(s) 274, 367
SfcI CTRYAG 1 cut(s) 862
Sfr274I CTCGAG 1 cut(s) 188
SlaI CTCGAG 1 cut(s) 188
SmlI CTYRAG 3 cut(s) 188, 203, 1375
SmoI CTYRAG 3 cut(s) 188, 203, 1375
SphI GCATGC 1 cut(s) 1157
Sse9I AATT 8 cut(s) 399, 497, 577, 657, 692, 992, 1146, 1330
SsiI CCGC 6 cut(s) 48, 113, 427, 935, 1038, 1424
SspMI CTAG 4 cut(s) 752, 794, 815, 1209
StyD4I CCNGG 1 cut(s) 117
TaaI ACNGT 5 cut(s) 35, 470, 708, 1237, 1396
TaqI TCGA 4 cut(s) 189, 357, 483, 741
TaqII GACCGA 1 cut(s) 153
TasI AATT 8 cut(s) 399, 497, 577, 657, 692, 992, 1146, 1330
TatI WGTACW 1 cut(s) 967
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 3 cut(s) 245, 1111, 1381
Tru1I TTAA 6 cut(s) 720, 887, 1275, 1311, 1419, 1440
Tru9I TTAA 6 cut(s) 720, 887, 1275, 1311, 1419, 1440
TscAI CASTG 3 cut(s) 40, 548, 977
TseFI GTSAC 1 cut(s) 1433
TseI GCWGC 1 cut(s) 1008
Tsp45I GTSAC 1 cut(s) 1433
TspDTI ATGAA 7 cut(s) 282, 343, 407, 479, 490, 612, 1235
TspRI CASTG 3 cut(s) 40, 548, 977
VspI ATTAAT 1 cut(s) 887
XagI CCTNNNNNAGG 1 cut(s) 818
XapI RAATTY 2 cut(s) 497, 692
XbaI TCTAGA 1 cut(s) 793
XceI RCATGY 2 cut(s) 635, 1157
XhoI CTCGAG 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 4 cut(s) 752, 794, 815, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.