Belongs to the universal ribosomal protein uS5 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 159 |
| AcsI | RAATTY | 1 cut(s) | 62 |
| AgsI | TTSAA | 3 cut(s) | 13, 67, 131 |
| AluBI | AGCT | 4 cut(s) | 38, 106, 139, 178 |
| AluI | AGCT | 4 cut(s) | 38, 106, 139, 178 |
| AlwI | GGATC | 1 cut(s) | 159 |
| ApoI | RAATTY | 1 cut(s) | 62 |
| AsuNHI | GCTAGC | 1 cut(s) | 139 |
| BfaI | CTAG | 3 cut(s) | 140, 182, 203 |
| BfmI | CTRYAG | 1 cut(s) | 247 |
| BglII | AGATCT | 1 cut(s) | 199 |
| BmtI | GCTAGC | 1 cut(s) | 143 |
| Bsp143I | GATC | 3 cut(s) | 151, 192, 199 |
| BspOI | GCTAGC | 1 cut(s) | 143 |
| BspPI | GGATC | 1 cut(s) | 159 |
| BssMI | GATC | 3 cut(s) | 151, 192, 199 |
| Bst6I | CTCTTC | 2 cut(s) | 183, 230 |
| BstC8I | GCNNGC | 1 cut(s) | 141 |
| BstKTI | GATC | 3 cut(s) | 154, 195, 202 |
| BstMBI | GATC | 3 cut(s) | 151, 192, 199 |
| BstSFI | CTRYAG | 1 cut(s) | 247 |
| BstX2I | RGATCY | 1 cut(s) | 199 |
| BstYI | RGATCY | 1 cut(s) | 199 |
| BtsI | GCAGTG | 1 cut(s) | 213 |
| BtsIMutI | CAGTG | 1 cut(s) | 213 |
| Cac8I | GCNNGC | 1 cut(s) | 141 |
| CviJI | RGCY | 4 cut(s) | 38, 106, 139, 178 |
| CviKI_1 | RGCY | 4 cut(s) | 38, 106, 139, 178 |
| DpnI | GATC | 3 cut(s) | 153, 194, 201 |
| DpnII | GATC | 3 cut(s) | 151, 192, 199 |
| Eam1104I | CTCTTC | 2 cut(s) | 183, 230 |
| EarI | CTCTTC | 2 cut(s) | 183, 230 |
| EcoRI | GAATTC | 1 cut(s) | 62 |
| FaiI | YATR | 2 cut(s) | 156, 249 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 162, 194 |
| FspBI | CTAG | 3 cut(s) | 140, 182, 203 |
| HindIII | AAGCTT | 1 cut(s) | 176 |
| Hpy188I | TCNGA | 2 cut(s) | 35, 197 |
| Hpy188III | TCNNGA | 1 cut(s) | 182 |
| Kzo9I | GATC | 3 cut(s) | 151, 192, 199 |
| LpnPI | CCDG | 1 cut(s) | 107 |
| MaeI | CTAG | 3 cut(s) | 140, 182, 203 |
| MalI | GATC | 3 cut(s) | 153, 194, 201 |
| MboI | GATC | 3 cut(s) | 151, 192, 199 |
| MboII | GAAGA | 7 cut(s) | 68, 71, 95, 143, 200, 244, 247 |
| MflI | RGATCY | 1 cut(s) | 199 |
| MluCI | AATT | 3 cut(s) | 27, 62, 67 |
| MmeI | TCCRAC | 1 cut(s) | 129 |
| MnlI | CCTC | 2 cut(s) | 73, 178 |
| NdeII | GATC | 3 cut(s) | 151, 192, 199 |
| NheI | GCTAGC | 1 cut(s) | 139 |
| PsuI | RGATCY | 1 cut(s) | 199 |
| Sau3AI | GATC | 3 cut(s) | 151, 192, 199 |
| SetI | ASST | 4 cut(s) | 40, 108, 141, 180 |
| SfcI | CTRYAG | 1 cut(s) | 247 |
| SgeI | CNNG | 9 cut(s) | 17, 47, 88, 106, 133, 140, 152, 194, 215 |
| Sse9I | AATT | 3 cut(s) | 27, 62, 67 |
| SspMI | CTAG | 3 cut(s) | 140, 182, 203 |
| TasI | AATT | 3 cut(s) | 27, 62, 67 |
| TscAI | CASTG | 1 cut(s) | 213 |
| TspDTI | ATGAA | 1 cut(s) | 69 |
| TspGWI | ACGGA | 1 cut(s) | 242 |
| TspRI | CASTG | 1 cut(s) | 213 |
| XapI | RAATTY | 1 cut(s) | 62 |
| XbaI | TCTAGA | 1 cut(s) | 181 |
| XspI | CTAG | 3 cut(s) | 140, 182, 203 |