Rh3CG283100

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001359
Physical Location & Seq
Forward (+)
28464163 .. 28467426
3264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG283100.1

Sequence Viewer

Length: 600 bp
ATGCGTGTCACACCATTGATAGAGAAGCTTGAGAAGGAGAAGCTCAAGACCACTGGGGATCTCAATTCCTATGAGGAGCCCACCGATTCTGATAGTGACGATGACGACGAGCGGTTTAGTCCCGATGCCATCAAGAAGAGGGTTGATGAGTTTCAGAGGAAGTTCATGCGACATGAGGAATTGCTCAAGAATTTCACACAGGCTGTTCCTAGAAATCAATGCAAAACTTTTGAACCTTGCAATGGATTCGATGTTAGATTAATTGATGTGAACCGGACTTGTAAAGTTACAAAGGAAGGGCAAGTGGTCAAATACACTGCTATATTAGTTTGTGGGAACTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGTGTTTCTCTGGCCAGCACCACTAGGACGGGTATGAAAGCGGGAAGAACTGTCGAAACCATTCTAAACTTGGCTGGTTTCAATAATGTCAAGTCTAAGGTTGTTGGTTCAAGGAGTCCTCATAATACAGTGAAGGCTGTTTTCAAAGCCCTTACTGTGATTGAAACTTCCAAGGATGTTCAAGAAAACTTTGGGCGCACTGTTGTTGAGAAGTACCTTCTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

199

Amino Acids

22.36

Weight (kDa)

9.08

Isoelectric Point (pI)

24.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 83 - 137 1.1e-11 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 133 - 186 2.5e-15 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 112
AciI CCGC 2 cut(s) 112, 413
AclWI GGATC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 190
AfaI GTAC 1 cut(s) 587
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 6 cut(s) 233, 454, 483, 517, 536, 554
AluBI AGCT 2 cut(s) 28, 43
AluI AGCT 2 cut(s) 28, 43
AlwI GGATC 1 cut(s) 66
AoxI GGCC 1 cut(s) 384
ApoI RAATTY 1 cut(s) 190
AseI ATTAAT 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 570
BalI TGGCCA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 81
BccI CCATC 1 cut(s) 137
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BfaI CTAG 2 cut(s) 210, 396
BmiI GGNNCC 1 cut(s) 78
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 63
BpuEI CTTGAG 3 cut(s) 29, 50, 170
BsaJI CCNNGG 1 cut(s) 543
BsaWI WCCGGW 1 cut(s) 273
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 543
BseGI GGATG 1 cut(s) 553
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 89
BshFI GGCC 1 cut(s) 386
BsiSI CCGG 1 cut(s) 274
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 105
BsnI GGCC 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 58
BspACI CCGC 2 cut(s) 112, 413
BspANI GGCC 1 cut(s) 386
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 66
BsrBI CCGCTC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 247
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 543
BssMI GATC 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 543
Bst4CI ACNGT 4 cut(s) 424, 502, 529, 574
Bst6I CTCTTC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 553
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 1 cut(s) 61
BstMBI GATC 1 cut(s) 58
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 1 cut(s) 386
BtsCI GGATG 1 cut(s) 553
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 4 cut(s) 51, 315, 507, 570
Cac8I GCNNGC 1 cut(s) 388
CfoI GCGC 1 cut(s) 570
Csp6I GTAC 1 cut(s) 586
CviAII CATG 3 cut(s) 166, 173, 344
CviJI RGCY 8 cut(s) 28, 43, 79, 203, 386, 446, 509, 521
CviKI_1 RGCY 8 cut(s) 28, 43, 79, 203, 386, 446, 509, 521
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 1 cut(s) 468
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 543
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 543
FaeI CATG 3 cut(s) 169, 176, 347
FaiI YATR 9 cut(s) 72, 167, 174, 323, 345, 360, 407, 495, 598
FaqI GGGAC 1 cut(s) 105
FatI CATG 3 cut(s) 165, 172, 343
FauI CCCGC 1 cut(s) 406
FokI GGATG 1 cut(s) 560
FriOI GRGCYC 1 cut(s) 81
FspBI CTAG 2 cut(s) 210, 396
GlaI GCGC 1 cut(s) 569
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 274
HhaI GCGC 1 cut(s) 570
Hin1II CATG 3 cut(s) 169, 176, 347
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 3 cut(s) 86, 246, 487
HpaII CCGG 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 91, 156
Hpy188III TCNNGA 5 cut(s) 46, 122, 133, 187, 554
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 4 cut(s) 28, 290, 499, 599
HpyCH4III ACNGT 4 cut(s) 424, 502, 529, 574
HpyCH4V TGCA 2 cut(s) 222, 240
HpyF3I CTNAG 1 cut(s) 468
Hsp92II CATG 3 cut(s) 169, 176, 347
HspAI GCGC 1 cut(s) 568
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 6 cut(s) 39, 185, 287, 368, 400, 432
LweI GCATC 1 cut(s) 115
MaeI CTAG 2 cut(s) 210, 396
MaeIII GTNAC 3 cut(s) 7, 95, 286
MalI GATC 1 cut(s) 60
MbiI CCGCTC 1 cut(s) 112
MboI GATC 1 cut(s) 58
MboII GAAGA 2 cut(s) 148, 429
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 81
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 4 cut(s) 64, 179, 190, 261
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 1 cut(s) 496
MnlI CCTC 5 cut(s) 67, 132, 150, 169, 501
Mox20I TGGCCA 1 cut(s) 386
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 386
MseI TTAA 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 1 cut(s) 274
NdeII GATC 1 cut(s) 58
NlaIII CATG 3 cut(s) 169, 176, 347
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 2 cut(s) 7, 95
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 2 cut(s) 86, 246
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PshBI ATTAAT 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 78
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
SaqAI TTAA 1 cut(s) 260
Sau3AI GATC 1 cut(s) 58
SchI GAGTC 1 cut(s) 496
SduI GDGCHC 1 cut(s) 81
SetI ASST 6 cut(s) 30, 45, 238, 376, 474, 591
SfaNI GCATC 1 cut(s) 115
SmlI CTYRAG 3 cut(s) 29, 44, 185
SmoI CTYRAG 3 cut(s) 29, 44, 185
Sse9I AATT 4 cut(s) 64, 179, 190, 261
SsiI CCGC 2 cut(s) 112, 413
SspMI CTAG 2 cut(s) 210, 396
StyI CCWWGG 1 cut(s) 543
TaaI ACNGT 4 cut(s) 424, 502, 529, 574
TaqI TCGA 2 cut(s) 249, 426
TasI AATT 4 cut(s) 64, 179, 190, 261
TfiI GAWTC 2 cut(s) 86, 246
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TscAI CASTG 4 cut(s) 58, 322, 507, 577
TseFI GTSAC 2 cut(s) 7, 95
Tsp45I GTSAC 2 cut(s) 7, 95
TspDTI ATGAA 2 cut(s) 154, 422
TspRI CASTG 4 cut(s) 58, 322, 507, 577
VspI ATTAAT 1 cut(s) 260
XapI RAATTY 1 cut(s) 190
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 2 cut(s) 210, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.