Rroxscaffold_4G00276480

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001359
Physical Location & Seq
Reverse (-)
58695 .. 63774
5080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00276480.1

Sequence Viewer

Length: 1482 bp
ATGTCCAAATCGCAAGCGTTCTCGTGGTCGCGTCTCATCTTCAATCAAGCAGTCAACAAAACCCGCAACGCCCCACTCCCACTCCCCTTCATCTCCTCCACCTTACCACACTTGCTCAGGTCCTCTGGGCAGACCCATTACTATTCCACAGTATCAGACAGAGTAGTCCGTGACCTTCTGGCCGAGGTCGAGAGAGAAAAGCAGAGAGAAAGGGAGCAGAGGAAAAGGGCTGGTCTCGACACCAAAGACATTGACCAAGAAGATGAGGAGGACTACATGCGTGTCACTCCATTGATAGAGAAGCTTGAGAAGGAGAAGCTCAAGGCCACTGGGGATCTCAATTCCTATGAGGAGCCCACCGATTCGGATAGTGACGACGACGACGAGCGCTTTACTCCCGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAGGAAGTTCACGCGACACGAGGAATTGCTCAAGAATTTCACACAGGCTGAGACACTTGATGATGCTTTTAAATGGATGAATAAAATTGACAAGTTTGAGCAAAAGCATTTCCGGCTTCGCCCTGAATATAGAGTCATTGGTGAATTGATGAACCGTCTAAAAGTAGCTGAGGGCAAGGATAAATTCATTCTGCAGCACAAACTAAACAGGGCTATGCGATTGGTGCAGTGGAAGGAAGCCTATGATCCAAACGATCCTGCCAACTATGGAGTCATTCAGGAACAACTGGAGCCCTCATTTGATGTTCTAGAAGATGCTGGATTTGAAAAAGAAAAAAAAATTATTCGAGGAGTTGATGATGATGATGATGAAGAATTCAATGACATGAAGGAGAAAGATGACATACTATTAGAGAAGCTAAATGCTATTGACAAGAAACTTGAAGAGAAGCTAGCAGAGTTGGATCATACTTTTGGAAAGAAGGGAAAGGTTCTAGAGGAAGAGATCAGAGATCTAGCAGAGGAAAGAAATGATTTGACGGAGAAGAAGAGAAGACCTCTCTATAGGAAAGGTTTTGATGTTAGATTAATTGATGTGAACCGGACTTGTAAAGTTACAAAGGGAGGACAAGTGGTCAAATACACTGCTATATTAGTTTGTGGGAACTATCATGGTGTTGTAGGTTATGCTAAAGCAAAAGGTCCAGCTGTCCCCCTCGCCCTCCAGAAGGCATATGAGAAATGCTTTCAGAATCTCCACTATGTAGAACGACATGAGGAGCATACAATTGCGCATGCAATACAAACTACATATAAAAAAACAAAGGTTTATCTCTGGCCTGCGCCCACTAGGACGGGTATGAAAGCAGGAAGAACTGTTGAAACCATTCTAAACTTGGCAGGTTTCAATAATGTCAAGTCTAAGGTTGTTGGTTCAAGGAATCCTCATAATACAGTGAAGGCTGTTTTCAAAGCCCTTAATGCGATTGAAACGCCCAAGGATGTTCAAGAAAAGTTTGGACGCACTGTTGTTGAGAACTACGTCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

493

Amino Acids

57.39

Weight (kDa)

7.67

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 336 - 400 1.7e-18 Ribosomal protein S5, N-terminal domain
Ribosomal_S5_C PF03719 414 - 482 2.7e-23 Ribosomal protein S5, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
Acc16I TGCGCA 1 cut(s) 1224
Acc36I ACCTGC 1 cut(s) 1322
AccII CGCG 2 cut(s) 31, 445
AciI CCGC 1 cut(s) 64
AclWI GGATC 4 cut(s) 342, 671, 680, 903
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 3 cut(s) 466, 614, 806
AfeI AGCGCT 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 1159
AhdI GACNNNNNGTC 1 cut(s) 1064
AluBI AGCT 6 cut(s) 304, 319, 599, 850, 883, 1139
AluI AGCT 6 cut(s) 304, 319, 599, 850, 883, 1139
Alw26I GTCTC 3 cut(s) 38, 239, 476
AlwI GGATC 4 cut(s) 342, 671, 680, 903
Aor51HI AGCGCT 1 cut(s) 389
AoxI GGCC 3 cut(s) 180, 324, 1268
ApeKI GCWGC 1 cut(s) 625
ApoI RAATTY 3 cut(s) 466, 614, 806
AseI ATTAAT 1 cut(s) 1019
Asp700I GAANNNNTTC 1 cut(s) 1317
AspLEI GCGC 3 cut(s) 390, 1225, 1276
AspS9I GGNCC 2 cut(s) 120, 1133
AsuHPI GGTGA 1 cut(s) 584
AsuNHI GCTAGC 1 cut(s) 883
AvaII GGWCC 2 cut(s) 120, 1133
BanII GRGCYC 2 cut(s) 357, 726
BauI CACGAG 2 cut(s) 22, 449
BbsI GAAGAC 1 cut(s) 991
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 1 cut(s) 637
BceAI ACGGC 1 cut(s) 389
BcoDI GTCTC 3 cut(s) 38, 239, 476
BfaI CTAG 5 cut(s) 740, 884, 926, 947, 1281
BfmI CTRYAG 2 cut(s) 623, 994
BfoI RGCGCY 1 cut(s) 391
BfuAI ACCTGC 1 cut(s) 1322
BglII AGATCT 1 cut(s) 943
BisI GCNGC 1 cut(s) 626
BlsI GCNGC 1 cut(s) 627
Bme18I GGWCC 2 cut(s) 120, 1133
BmeRI GACNNNNNGTC 1 cut(s) 1064
BmgT120I GGNCC 2 cut(s) 120, 1133
BmiI GGNNCC 2 cut(s) 354, 723
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 3 cut(s) 391, 484, 736
BmtI GCTAGC 1 cut(s) 887
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 991
BplI GAGNNNNNCTC 2 cut(s) 973, 1005
BpmI CTGGAG 2 cut(s) 740, 1139
Bpu10I CCTNAGC 2 cut(s) 116, 600
BpuEI CTTGAG 3 cut(s) 305, 326, 446
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 183, 1428
BsaWI WCCGGW 2 cut(s) 1032, 1476
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 1047, 1077
Bsc4I CCNNNNNNNGG 1 cut(s) 1159
Bse1I ACTGG 2 cut(s) 334, 723
BseDI CCNNGG 2 cut(s) 183, 1428
BseGI GGATG 2 cut(s) 513, 1438
BseLI CCNNNNNNNGG 1 cut(s) 1159
BseMII CTCAG 3 cut(s) 130, 471, 591
BseNI ACTGG 2 cut(s) 334, 723
BseRI GAGGAG 5 cut(s) 85, 281, 365, 795, 1223
BseXI GCAGC 1 cut(s) 637
BsgI GTGCAG 1 cut(s) 677
Bsh1236I CGCG 2 cut(s) 31, 445
BshFI GGCC 3 cut(s) 182, 326, 1270
BsiSI CCGG 3 cut(s) 544, 1033, 1477
BslFI GGGAC 1 cut(s) 1127
BslI CCNNNNNNNGG 1 cut(s) 1159
BsmAI GTCTC 3 cut(s) 38, 239, 476
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 1127
BsnI GGCC 3 cut(s) 182, 326, 1270
Bso31I GGTCTC 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 357, 726
Bsp143I GATC 6 cut(s) 334, 676, 685, 895, 936, 943
BspACI CCGC 1 cut(s) 64
BspANI GGCC 3 cut(s) 182, 326, 1270
BspCNI CTCAG 3 cut(s) 129, 472, 592
BspFNI CGCG 2 cut(s) 31, 445
BspLI GGNNCC 2 cut(s) 354, 723
BspMAI CTGCAG 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 1322
BspOI GCTAGC 1 cut(s) 887
BspPI GGATC 4 cut(s) 342, 671, 680, 903
BspTNI GGTCTC 1 cut(s) 239
BsrI ACTGG 2 cut(s) 334, 723
BssECI CCNNGG 2 cut(s) 183, 1428
BssMI GATC 6 cut(s) 334, 676, 685, 895, 936, 943
BssSI CACGAG 2 cut(s) 22, 449
BssT1I CCWWGG 1 cut(s) 1428
Bst2BI CACGAG 2 cut(s) 22, 449
Bst4CI ACNGT 5 cut(s) 151, 587, 1309, 1387, 1459
Bst6I CTCTTC 4 cut(s) 407, 870, 927, 974
BstC8I GCNNGC 4 cut(s) 15, 885, 1227, 1272
BstDEI CTNAG 4 cut(s) 116, 480, 600, 1353
BstENI CCTNNNNNAGG 1 cut(s) 1157
BstF5I GGATG 2 cut(s) 513, 1438
BstFNI CGCG 2 cut(s) 31, 445
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 3 cut(s) 390, 1225, 1276
BstKTI GATC 6 cut(s) 337, 679, 688, 898, 939, 946
BstMAI GTCTC 3 cut(s) 38, 239, 476
BstMBI GATC 6 cut(s) 334, 676, 685, 895, 936, 943
BstMWI GCNNNNNNNGC 3 cut(s) 544, 655, 1412
BstNSI RCATGY 2 cut(s) 280, 1229
BstSFI CTRYAG 2 cut(s) 623, 994
BstUI CGCG 2 cut(s) 31, 445
BstV1I GCAGC 1 cut(s) 637
BstV2I GAAGAC 1 cut(s) 991
BstX2I RGATCY 2 cut(s) 334, 943
BstYI RGATCY 2 cut(s) 334, 943
BsuRI GGCC 3 cut(s) 182, 326, 1270
BtsCI GGATG 2 cut(s) 513, 1438
BtsI GCAGTG 2 cut(s) 665, 1074
BtsIMutI CAGTG 5 cut(s) 327, 665, 1074, 1392, 1455
BveI ACCTGC 1 cut(s) 1322
Cac8I GCNNGC 4 cut(s) 15, 885, 1227, 1272
CfoI GCGC 3 cut(s) 390, 1225, 1276
Cfr13I GGNCC 2 cut(s) 120, 1133
CseI GACGC 2 cut(s) 20, 1461
CviAII CATG 5 cut(s) 277, 817, 1103, 1205, 1226
DdeI CTNAG 4 cut(s) 116, 480, 600, 1353
DpnI GATC 6 cut(s) 336, 678, 687, 897, 938, 945
DpnII GATC 6 cut(s) 334, 676, 685, 895, 936, 943
DraI TTTAAA 1 cut(s) 502
DrdI GACNNNNNNGTC 1 cut(s) 164
DriI GACNNNNNGTC 1 cut(s) 1064
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 4 cut(s) 407, 870, 927, 974
Eam1105I GACNNNNNGTC 1 cut(s) 1064
EarI CTCTTC 4 cut(s) 407, 870, 927, 974
Eco130I CCWWGG 1 cut(s) 1428
Eco24I GRGCYC 2 cut(s) 357, 726
Eco31I GGTCTC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 120, 1133
Eco47III AGCGCT 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 1157
EcoO109I RGGNCCY 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 1428
EcoT38I GRGCYC 2 cut(s) 357, 726
ErhI CCWWGG 1 cut(s) 1428
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 5 cut(s) 280, 820, 1106, 1208, 1229
FaqI GGGAC 1 cut(s) 1127
FatI CATG 5 cut(s) 276, 816, 1102, 1204, 1225
FauI CCCGC 1 cut(s) 71
FauNDI CATATG 1 cut(s) 1165
Fnu4HI GCNGC 1 cut(s) 626
FokI GGATG 2 cut(s) 520, 1445
FriOI GRGCYC 2 cut(s) 357, 726
Fsp4HI GCNGC 1 cut(s) 626
FspBI CTAG 5 cut(s) 740, 884, 926, 947, 1281
FspI TGCGCA 1 cut(s) 1224
GlaI GCGC 3 cut(s) 389, 1224, 1275
GluI GCNGC 1 cut(s) 626
GsuI CTGGAG 2 cut(s) 740, 1139
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 3 cut(s) 182, 326, 1270
HapII CCGG 3 cut(s) 544, 1033, 1477
HgaI GACGC 2 cut(s) 20, 1461
HhaI GCGC 3 cut(s) 390, 1225, 1276
Hin1II CATG 5 cut(s) 280, 820, 1106, 1208, 1229
Hin6I GCGC 3 cut(s) 388, 1223, 1274
HinP1I GCGC 3 cut(s) 388, 1223, 1274
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 5 cut(s) 362, 564, 702, 1183, 1372
HpaII CCGG 3 cut(s) 544, 1033, 1477
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 3 cut(s) 55, 441, 1030
Hpy188I TCNGA 5 cut(s) 157, 367, 432, 941, 1182
Hpy8I GTNNAC 3 cut(s) 55, 441, 1030
Hpy99I CGWCG 3 cut(s) 380, 383, 386
HpyAV CCTTC 8 cut(s) 97, 185, 304, 658, 814, 907, 1153, 1384
HpyCH4III ACNGT 5 cut(s) 151, 587, 1309, 1387, 1459
HpyCH4IV ACGT 1 cut(s) 1473
HpyCH4V TGCA 3 cut(s) 625, 658, 1229
HpyF10VI GCNNNNNNNGC 3 cut(s) 544, 655, 1412
HpyF3I CTNAG 4 cut(s) 116, 480, 600, 1353
HpySE526I ACGT 1 cut(s) 1473
Hsp92II CATG 5 cut(s) 280, 820, 1106, 1208, 1229
HspAI GCGC 3 cut(s) 388, 1223, 1274
Kzo9I GATC 6 cut(s) 334, 676, 685, 895, 936, 943
LmnI GCTCC 4 cut(s) 214, 352, 721, 1210
Lsp1109I GCAGC 1 cut(s) 637
LweI GCATC 3 cut(s) 391, 484, 736
MaeI CTAG 5 cut(s) 740, 884, 926, 947, 1281
MaeII ACGT 1 cut(s) 1473
MaeIII GTNAC 4 cut(s) 170, 283, 371, 1045
MalI GATC 6 cut(s) 336, 678, 687, 897, 938, 945
MboI GATC 6 cut(s) 334, 676, 685, 895, 936, 943
MfeI CAATTG 1 cut(s) 1218
MflI RGATCY 2 cut(s) 334, 943
MhlI GDGCHC 2 cut(s) 357, 726
MlyI GAGTC 2 cut(s) 573, 711
MmeI TCCRAC 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 1317
MseI TTAA 3 cut(s) 501, 1019, 1410
MspA1I CMGCKG 1 cut(s) 1139
MspI CCGG 3 cut(s) 544, 1033, 1477
MunI CAATTG 1 cut(s) 1218
MvnI CGCG 2 cut(s) 31, 445
MwoI GCNNNNNNNGC 3 cut(s) 544, 655, 1412
NdeI CATATG 1 cut(s) 1165
NdeII GATC 6 cut(s) 334, 676, 685, 895, 936, 943
NheI GCTAGC 1 cut(s) 883
NlaIII CATG 5 cut(s) 280, 820, 1106, 1208, 1229
NlaIV GGNNCC 2 cut(s) 354, 723
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 3 cut(s) 170, 283, 371
NsbI TGCGCA 1 cut(s) 1224
NspI RCATGY 2 cut(s) 280, 1229
PaeI GCATGC 1 cut(s) 1229
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 1317
PfeI GAWTC 3 cut(s) 362, 1183, 1372
PkrI GCNGC 1 cut(s) 627
PleI GAGTC 2 cut(s) 572, 710
PpsI GAGTC 2 cut(s) 572, 710
PpuMI RGGWCCY 1 cut(s) 120
PshBI ATTAAT 1 cut(s) 1019
Psp5II RGGWCCY 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 354, 723
PspPI GGNCC 2 cut(s) 120, 1133
PspPPI RGGWCCY 1 cut(s) 120
PstI CTGCAG 1 cut(s) 627
PsuI RGATCY 2 cut(s) 334, 943
PvuII CAGCTG 1 cut(s) 1139
SaqAI TTAA 3 cut(s) 501, 1019, 1410
SatI GCNGC 1 cut(s) 626
Sau3AI GATC 6 cut(s) 334, 676, 685, 895, 936, 943
Sau96I GGNCC 2 cut(s) 120, 1133
SchI GAGTC 2 cut(s) 573, 711
SduI GDGCHC 2 cut(s) 357, 726
SfaNI GCATC 3 cut(s) 391, 484, 736
SfcI CTRYAG 2 cut(s) 623, 994
SinI GGWCC 2 cut(s) 120, 1133
SmlI CTYRAG 3 cut(s) 305, 320, 461
SmoI CTYRAG 3 cut(s) 305, 320, 461
SphI GCATGC 1 cut(s) 1229
SsiI CCGC 1 cut(s) 64
SspMI CTAG 5 cut(s) 740, 884, 926, 947, 1281
StyI CCWWGG 1 cut(s) 1428
TaaI ACNGT 5 cut(s) 151, 587, 1309, 1387, 1459
TaiI ACGT 1 cut(s) 1476
TaqI TCGA 3 cut(s) 189, 237, 778
TfiI GAWTC 3 cut(s) 362, 1183, 1372
Tru1I TTAA 3 cut(s) 501, 1019, 1410
Tru9I TTAA 3 cut(s) 501, 1019, 1410
TscAI CASTG 5 cut(s) 334, 665, 1081, 1392, 1462
TseFI GTSAC 3 cut(s) 170, 283, 371
TseI GCWGC 1 cut(s) 625
Tsp45I GTSAC 3 cut(s) 170, 283, 371
TspDTI ATGAA 7 cut(s) 79, 524, 596, 607, 816, 833, 1307
TspGWI ACGGA 2 cut(s) 158, 986
TspRI CASTG 5 cut(s) 334, 665, 1081, 1392, 1462
VpaK11BI GGWCC 2 cut(s) 120, 1133
VspI ATTAAT 1 cut(s) 1019
XagI CCTNNNNNAGG 1 cut(s) 1157
XapI RAATTY 3 cut(s) 466, 614, 806
XbaI TCTAGA 2 cut(s) 739, 925
XceI RCATGY 2 cut(s) 280, 1229
XcmI CCANNNNNNNNNTGG 1 cut(s) 1324
XmnI GAANNNNTTC 1 cut(s) 1317
XspI CTAG 5 cut(s) 740, 884, 926, 947, 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.