Rroxscaffold_2G00131740

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001359
Physical Location & Seq
Reverse (-)
68515346 .. 68518656
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131740.1

Sequence Viewer

Length: 486 bp
ATGAAGGAGAAAGATGACAAACTGTTAGAAAAGCCAAATGCTATTGACAAGAAACTTGAAGAAAAGCTAGCAGAGTTGGATCATACTTTTGGAAAGAAAGAAAAGGTTCTAGAGAAAGAGATCAGAGATCTAGCAGAGGAAAGAAATGATTTGACCGAGAAGAAGAGAAGCCCTCTCTATAGAAAAAACCGGTCTAGTGCAACAAGTGCGGTCGCTAGTAGCTTTGAAGGGCGACCCTATGGTCACCATGGTCTCCTGCTGGGGAGGCTGCCGGACCGATTAGAAGGTGGCGGGCAGTTCTCGGAAGGGCCGGATTCGGCGGCCGGGTGTGAGGAGTGTAGGGTGAGGGCAGCGGGTCATGGGCCTCTGCCTTCTTGGGCTGCAGAGATGCAATCTAGAGAATTGGTCCTAGGTGTGTTGAATGTTGTGACGTACACCAAAAGGGTCTTAGGCGTCAAGATGTTCCGGAAATTGGTTCCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

161

Amino Acids

18.03

Weight (kDa)

9.04

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 240
AccIII TCCGGA 1 cut(s) 465
AciI CCGC 4 cut(s) 209, 291, 320, 353
AclWI GGATC 1 cut(s) 87
AcoI YGGCCR 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 1 cut(s) 472
AgeI ACCGGT 1 cut(s) 189
AgsI TTSAA 3 cut(s) 59, 227, 421
AluBI AGCT 2 cut(s) 67, 222
AluI AGCT 2 cut(s) 67, 222
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 87
Aor13HI TCCGGA 1 cut(s) 465
AoxI GGCC 3 cut(s) 308, 321, 362
ApeKI GCWGC 3 cut(s) 268, 350, 380
AsiGI ACCGGT 1 cut(s) 189
Asp700I GAANNNNTTC 1 cut(s) 105
AspA2I CCTAGG 1 cut(s) 409
AspS9I GGNCC 4 cut(s) 274, 308, 362, 406
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 236, 355
AsuNHI GCTAGC 1 cut(s) 67
AvaII GGWCC 2 cut(s) 274, 406
AvrII CCTAGG 1 cut(s) 409
BbvI GCAGC 3 cut(s) 255, 362, 367
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 7 cut(s) 68, 110, 131, 195, 216, 396, 410
BfmI CTRYAG 2 cut(s) 178, 381
BglII AGATCT 1 cut(s) 127
BisI GCNGC 4 cut(s) 269, 321, 351, 381
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 4 cut(s) 270, 322, 352, 382
Bme1390I CCNGG 1 cut(s) 325
Bme18I GGWCC 2 cut(s) 274, 406
BmgT120I GGNCC 4 cut(s) 274, 308, 362, 406
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 378
BmtI GCTAGC 1 cut(s) 71
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpuMI CCSGG 1 cut(s) 325
BsaHI GRCGYC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 247, 409
BsaWI WCCGGW 2 cut(s) 189, 465
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse118I RCCGGY 1 cut(s) 189
BseAI TCCGGA 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 247, 409
BseLI CCNNNNNNNGG 1 cut(s) 472
BseRI GAGGAG 1 cut(s) 347
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 3 cut(s) 255, 362, 367
BseYI CCCAGC 1 cut(s) 259
Bsh1285I CGRYCG 2 cut(s) 213, 324
BshFI GGCC 3 cut(s) 310, 323, 364
BshTI ACCGGT 1 cut(s) 189
BsiEI CGRYCG 2 cut(s) 213, 324
BsiSI CCGG 5 cut(s) 190, 272, 311, 324, 466
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 257
BsnI GGCC 3 cut(s) 310, 323, 364
Bso31I GGTCTC 1 cut(s) 257
Bsp13I TCCGGA 1 cut(s) 465
Bsp143I GATC 3 cut(s) 79, 120, 127
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 4 cut(s) 209, 291, 320, 353
BspANI GGCC 3 cut(s) 310, 323, 364
BspEI TCCGGA 1 cut(s) 465
BspLI GGNNCC 1 cut(s) 477
BspMAI CTGCAG 1 cut(s) 385
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 257
BsrFI RCCGGY 1 cut(s) 189
BssAI RCCGGY 1 cut(s) 189
BssECI CCNNGG 2 cut(s) 247, 409
BssMI GATC 3 cut(s) 79, 120, 127
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 247, 409
Bst4CI ACNGT 1 cut(s) 24
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 453
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 2 cut(s) 69, 293
BstDEI CTNAG 1 cut(s) 448
BstDSI CCRYGG 1 cut(s) 247
BstEII GGTNACC 1 cut(s) 242
BstKTI GATC 3 cut(s) 82, 123, 130
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 3 cut(s) 79, 120, 127
BstMCI CGRYCG 2 cut(s) 213, 324
BstMWI GCNNNNNNNGC 2 cut(s) 206, 265
BstPI GGTNACC 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 2 cut(s) 178, 381
BstV1I GCAGC 3 cut(s) 255, 362, 367
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BstZI CGGCCG 1 cut(s) 321
BsuRI GGCC 3 cut(s) 310, 323, 364
BtgI CCRYGG 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 69, 293
Cfr10I RCCGGY 1 cut(s) 189
Cfr13I GGNCC 4 cut(s) 274, 308, 362, 406
CpoI CGGWCCG 1 cut(s) 274
CseI GACGC 1 cut(s) 442
Csp6I GTAC 1 cut(s) 433
CspAI ACCGGT 1 cut(s) 189
CspI CGGWCCG 1 cut(s) 274
CviAII CATG 2 cut(s) 248, 359
CviJI RGCY 9 cut(s) 34, 67, 171, 222, 268, 310, 323, 364, 380
CviKI_1 RGCY 9 cut(s) 34, 67, 171, 222, 268, 310, 323, 364, 380
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 1 cut(s) 448
DpnI GATC 3 cut(s) 81, 122, 129
DpnII GATC 3 cut(s) 79, 120, 127
DrdI GACNNNNNNGTC 1 cut(s) 240
DseDI GACNNNNNNGTC 1 cut(s) 240
EaeI YGGCCR 1 cut(s) 321
EagI CGGCCG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 247, 409
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 274, 406
Eco52I CGGCCG 1 cut(s) 321
Eco91I GGTNACC 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 247, 409
ErhI CCWWGG 2 cut(s) 247, 409
FaeI CATG 2 cut(s) 251, 362
FaiI YATR 5 cut(s) 84, 180, 240, 249, 360
FatI CATG 2 cut(s) 247, 358
FauI CCCGC 2 cut(s) 284, 346
Fnu4HI GCNGC 4 cut(s) 269, 321, 351, 381
Fsp4HI GCNGC 4 cut(s) 269, 321, 351, 381
FspBI CTAG 7 cut(s) 68, 110, 131, 195, 216, 396, 410
GluI GCNGC 4 cut(s) 269, 321, 351, 381
GsaI CCCAGC 1 cut(s) 263
HaeIII GGCC 3 cut(s) 310, 323, 364
HapII CCGG 5 cut(s) 190, 272, 311, 324, 466
HgaI GACGC 1 cut(s) 442
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 2 cut(s) 251, 362
HinfI GANTC 1 cut(s) 314
HpaII CCGG 5 cut(s) 190, 272, 311, 324, 466
HphI GGTGA 2 cut(s) 236, 355
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 2 cut(s) 125, 304
Hpy188III TCNNGA 4 cut(s) 110, 396, 457, 466
Hpy8I GTNNAC 1 cut(s) 435
HpyAV CCTTC 4 cut(s) 221, 278, 299, 381
HpyCH4III ACNGT 1 cut(s) 24
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 3 cut(s) 200, 383, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 265
HpyF3I CTNAG 1 cut(s) 448
HpySE526I ACGT 1 cut(s) 431
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 2 cut(s) 251, 362
Kpn2I TCCGGA 1 cut(s) 465
Kzo9I GATC 3 cut(s) 79, 120, 127
LpnPI CCDG 7 cut(s) 203, 245, 269, 285, 324, 337, 479
Lsp1109I GCAGC 3 cut(s) 255, 362, 367
LweI GCATC 1 cut(s) 378
MaeI CTAG 7 cut(s) 68, 110, 131, 195, 216, 396, 410
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 2 cut(s) 242, 427
MalI GATC 3 cut(s) 81, 122, 129
MboI GATC 3 cut(s) 79, 120, 127
MboII GAAGA 3 cut(s) 71, 172, 175
MflI RGATCY 1 cut(s) 127
MluCI AATT 2 cut(s) 401, 470
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 6 cut(s) 130, 183, 258, 325, 339, 375
MroI TCCGGA 1 cut(s) 465
MroXI GAANNNNTTC 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 5 cut(s) 190, 272, 311, 324, 466
MspR9I CCNGG 1 cut(s) 325
MwoI GCNNNNNNNGC 2 cut(s) 206, 265
NciI CCSGG 1 cut(s) 325
NcoI CCATGG 1 cut(s) 247
NdeII GATC 3 cut(s) 79, 120, 127
NheI GCTAGC 1 cut(s) 67
NlaIII CATG 2 cut(s) 251, 362
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 2 cut(s) 242, 427
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 189
PkrI GCNGC 4 cut(s) 270, 322, 352, 382
PspEI GGTNACC 1 cut(s) 242
PspFI CCCAGC 1 cut(s) 259
PspN4I GGNNCC 1 cut(s) 477
PspPI GGNCC 4 cut(s) 274, 308, 362, 406
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
Rsr2I CGGWCCG 1 cut(s) 274
RsrII CGGWCCG 1 cut(s) 274
SatI GCNGC 4 cut(s) 269, 321, 351, 381
Sau3AI GATC 3 cut(s) 79, 120, 127
Sau96I GGNCC 4 cut(s) 274, 308, 362, 406
ScrFI CCNGG 1 cut(s) 325
SetI ASST 6 cut(s) 69, 108, 224, 289, 415, 434
SfaNI GCATC 1 cut(s) 378
SfcI CTRYAG 2 cut(s) 178, 381
SinI GGWCC 2 cut(s) 274, 406
Sse9I AATT 2 cut(s) 401, 470
SsiI CCGC 4 cut(s) 209, 291, 320, 353
SspMI CTAG 7 cut(s) 68, 110, 131, 195, 216, 396, 410
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 2 cut(s) 247, 409
TaaI ACNGT 1 cut(s) 24
TaiI ACGT 1 cut(s) 434
TaqII GACCGA 2 cut(s) 170, 291
TasI AATT 2 cut(s) 401, 470
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 1 cut(s) 314
TseFI GTSAC 2 cut(s) 242, 427
TseI GCWGC 3 cut(s) 268, 350, 380
Tsp45I GTSAC 2 cut(s) 242, 427
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 274, 406
XbaI TCTAGA 2 cut(s) 109, 395
XmaJI CCTAGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 7 cut(s) 68, 110, 131, 195, 216, 396, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.