Rh3AG249900

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001359
Physical Location & Seq
Reverse (-)
26914443 .. 26917668
3226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG249900.1

Sequence Viewer

Length: 594 bp
ATGCGTGTCACACCATTGATAGAGAAGCTTGAGAAGGAGAAGCTCGAGACCACTGGGGATCTCAATTCCTATGAGGAGCCCACTGATTCTGATAGTGACGATGACGACGAGCGGGTCAGTCCTGATGCCATCAAGAAGAGGGTTGATGAGTTTCAGAGGAAGTTCACGCGACATGAGGAATTGCTCAAGAATTTCACACAGGCTGGTATTACTCTTCTTCATTGTGGAGGGCAAGTGGTCAAATACACTGCTATATTAGTTTGTGGGAACTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGTCCAGCCGTCCCTATTGCCCTCCAGAAGGCATATGAGAAATGCTTTCAGAATCTCCATCATGTAGAACGACATGAGGAGCATACAATTGCGCATGCAATACAAACTACATATAAAAAAACGAAGGTGTATCTCTGGCCTGCACCACTAGGACGGTCTAAGGTTGTTGGTTCAAGGAGTCCTCATAATACAGTGAAGGTTGTTTTCAAAGCCCTTAATGTGATTGAAACTTCCAAGGATGTTCAAGAAAATTTTGGGCGCACTGTTGTTGAGAAGTACCTTCTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

197

Amino Acids

22.33

Weight (kDa)

8.32

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S5 PF00333 76 - 124 8e-12 Ribosomal protein S5, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
Acc16I TGCGCA 1 cut(s) 396
AccBSI CCGCTC 1 cut(s) 112
AccII CGCG 1 cut(s) 169
AciI CCGC 1 cut(s) 112
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 2 cut(s) 190, 553
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 4 cut(s) 477, 511, 530, 548
AluBI AGCT 2 cut(s) 28, 43
AluI AGCT 2 cut(s) 28, 43
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 440
ApoI RAATTY 2 cut(s) 190, 553
AspLEI GCGC 2 cut(s) 397, 564
AspS9I GGNCC 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 81
BccI CCATC 2 cut(s) 137, 369
BceAI ACGGC 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 305
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 78
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 50, 170
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 537
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 1 cut(s) 547
BseLI CCNNNNNNNGG 1 cut(s) 331
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 89, 395
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 169
BshFI GGCC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 1 cut(s) 442
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 58
BspACI CCGC 1 cut(s) 112
BspANI GGCC 1 cut(s) 442
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 66
BspTNI GGTCTC 1 cut(s) 41
BsrBI CCGCTC 1 cut(s) 112
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 537
Bst4CI ACNGT 3 cut(s) 459, 496, 568
Bst6I CTCTTC 2 cut(s) 131, 219
BstC8I GCNNGC 2 cut(s) 399, 444
BstDEI CTNAG 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 547
BstFNI CGCG 1 cut(s) 169
BstHHI GCGC 2 cut(s) 397, 564
BstKTI GATC 1 cut(s) 61
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 401
BstUI CGCG 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 1 cut(s) 442
BtsCI GGATG 1 cut(s) 547
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 5 cut(s) 51, 81, 246, 501, 564
Cac8I GCNNGC 2 cut(s) 399, 444
CfoI GCGC 2 cut(s) 397, 564
Cfr13I GGNCC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 580
CviAII CATG 5 cut(s) 173, 275, 365, 377, 398
CviJI RGCY 7 cut(s) 28, 43, 79, 203, 311, 442, 515
CviKI_1 RGCY 7 cut(s) 28, 43, 79, 203, 311, 442, 515
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 1 cut(s) 462
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
Eam1104I CTCTTC 2 cut(s) 131, 219
EarI CTCTTC 2 cut(s) 131, 219
Eco130I CCWWGG 1 cut(s) 537
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 5 cut(s) 176, 278, 368, 380, 401
FaqI GGGAC 1 cut(s) 299
FatI CATG 5 cut(s) 172, 274, 364, 376, 397
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 1 cut(s) 337
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 1 cut(s) 81
FspBI CTAG 1 cut(s) 452
FspI TGCGCA 1 cut(s) 396
GlaI GCGC 2 cut(s) 396, 563
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 1 cut(s) 442
HhaI GCGC 2 cut(s) 397, 564
Hin1II CATG 5 cut(s) 176, 278, 368, 380, 401
Hin6I GCGC 2 cut(s) 395, 562
HinP1I GCGC 2 cut(s) 395, 562
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 3 cut(s) 86, 355, 481
Hpy166II GTNNAC 1 cut(s) 165
Hpy188I TCNGA 3 cut(s) 91, 156, 354
Hpy188III TCNNGA 6 cut(s) 46, 122, 133, 187, 328, 548
Hpy8I GTNNAC 1 cut(s) 165
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 5 cut(s) 28, 325, 421, 493, 593
HpyCH4III ACNGT 3 cut(s) 459, 496, 568
HpyCH4V TGCA 2 cut(s) 401, 446
HpyF3I CTNAG 1 cut(s) 462
Hsp92II CATG 5 cut(s) 176, 278, 368, 380, 401
HspAI GCGC 2 cut(s) 395, 562
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 2 cut(s) 76, 382
LpnPI CCDG 8 cut(s) 39, 135, 185, 189, 321, 341, 424, 456
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 452
MaeIII GTNAC 2 cut(s) 7, 95
MalI GATC 1 cut(s) 60
MbiI CCGCTC 1 cut(s) 112
MboI GATC 1 cut(s) 58
MboII GAAGA 3 cut(s) 148, 206, 209
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 5 cut(s) 64, 179, 190, 390, 553
MlyI GAGTC 1 cut(s) 490
MnlI CCTC 8 cut(s) 67, 132, 150, 169, 221, 335, 373, 495
MseI TTAA 1 cut(s) 519
MunI CAATTG 1 cut(s) 390
MvnI CGCG 1 cut(s) 169
NdeI CATATG 1 cut(s) 337
NdeII GATC 1 cut(s) 58
NlaIII CATG 5 cut(s) 176, 278, 368, 380, 401
NlaIV GGNNCC 1 cut(s) 78
NmuCI GTSAC 2 cut(s) 7, 95
NsbI TGCGCA 1 cut(s) 396
NspI RCATGY 1 cut(s) 401
PaeI GCATGC 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 44
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 2 cut(s) 86, 355
PleI GAGTC 1 cut(s) 489
PpsI GAGTC 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SaqAI TTAA 1 cut(s) 519
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 81
SetI ASST 7 cut(s) 30, 45, 307, 432, 468, 504, 585
SfaNI GCATC 1 cut(s) 115
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 1 cut(s) 305
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 3 cut(s) 29, 44, 185
SmoI CTYRAG 3 cut(s) 29, 44, 185
SphI GCATGC 1 cut(s) 401
Sse9I AATT 5 cut(s) 64, 179, 190, 390, 553
SsiI CCGC 1 cut(s) 112
SspMI CTAG 1 cut(s) 452
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 3 cut(s) 459, 496, 568
TaqI TCGA 1 cut(s) 45
TasI AATT 5 cut(s) 64, 179, 190, 390, 553
TfiI GAWTC 2 cut(s) 86, 355
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 5 cut(s) 58, 88, 253, 501, 571
TseFI GTSAC 2 cut(s) 7, 95
Tsp45I GTSAC 2 cut(s) 7, 95
TspDTI ATGAA 1 cut(s) 209
TspRI CASTG 5 cut(s) 58, 88, 253, 501, 571
VpaK11BI GGWCC 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 2 cut(s) 190, 553
XceI RCATGY 1 cut(s) 401
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.