pycom07g28350

Belongs to the universal ribosomal protein uS5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001359
Physical Location & Seq
Reverse (-)
27679549 .. 27683577
4029 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g28350.5

Sequence Viewer

Length: 1506 bp
ATGAGCAAATCACAAGGTTTCTCGTGGTCACGTCTCATCGTGAAACAAGGCATCAAGAATCCCAGCAAAGCCCCACTCCCCTTGTCCCGGAAAATCTCCACTCGGCCACACTTTCCCGCTACCCCTCATCCACCCAAATGGTCGTTTTCCAGCAAAATCCCTTATTCAACAGTGTCATCCAGAGCGGCCCAAGACCTTCTGGCGGAGGTGGAGAGAGAAAAGCAGAGAGAACGAGAGCAGAGGAAGAGGGCGGGCCTGGACACCAAAGACATTGACCGAGAAGATGAAGAGGACTACATGGGCGTTATGCCATTGATTGAGAAGCTCGAGAAGGAGAAGCTCAAGGACACCGGAGACTTGAACTTGTACGAGGAGCCCACCGATTCCGATAGCGACGAAGACGATGAAAGGTTCACTCCCGATGCCGTCAAGAAGAGGTTTGATGAGTTTCAGAGGAAGTTCACGCGCCACGAGGAGTTGCTCAACAACTTCACCGAGGCTGAGACACTTGACGATGCTTATAAATGGATGAATAAAATTGACAAGTTTGAGCAAAAACATTTCCGCCTTCGCCCCGAATATAGGGTCATTGGTGAGTTGATGAACCGTCTCAAAGTAGCTGAGGGAAAGGACAAATTCATCCTCCAGCAGAAACTAAACAGGGCTATGAGATTGGTGCAGTGGAAGGAAGCCTACGATCCAAACAATCCTGCCAATTATGGAGTCATCCAACATGAACAAGTGGAGCCCTCAGTTGATTTATTGGAACATGCTGGGTTTGAAAAAGAAAAGCAAATTATACAAGGAGTTGATGACGACGACGAGGAAGAATTCAATGACATGAAGGAGAAAGATGACATACTGTTAGAGAAGCTTAATGCCATTGACAAAAAACTCGAAGAGAAGCTAGCAGAGTTAGATCATACATTTGGGAAGAAGGGTAAGGTTCTAGAGGAAGAGATCAGAGACCTAGCAGAGGAAAGAAATGATTTGACAGAGAAGAAGAGGAGACCTCTCTACAGGAAAGGGTTTGATGTTAAATTAATCAATGTGAACCGAACTTGTAAAGTAACAAAGGGGGGGCAAGTGGTCAAGTACACTGCCATATTAGCTTGTGGCAATTATCATGGTGTTGTTGGTTATGCTAAAGCAAAAGGCCCAGCAGTCCCTATCGCTCTTCAGAAGGCATATGAGAAGTGCTTTCAGAATCTCCACTACATTGAACGACACGAGGAGCATACAATTGCGCATGCAATACAAACAAGTTATAAAAAAACAAAGGTGTATCTCTGGCCTGCACCCACTAGGACGGGTATGAAAGCAGGAAGAACCGTTGAAACCATTCTAAACTTAGCTGGTTTCAAAAATGTTAAGTCTAAGGTTGTAGGCTCAAGGAATCCTTATAACACAGTCAAGGCTCTCTTCAAATCCCTTAATGCGATTGAAACTCGAAAGGATGTTCAAGATAAGTTTGGCCGCACTGTTGTTGAGAAGTACCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S5 Ribosomal_S5_C

Protein Analysis

502

Amino Acids

58.19

Weight (kDa)

8.74

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 522, 1269, 1404
Acc16I TGCGCA 1 cut(s) 1248
AccBSI CCGCTC 1 cut(s) 185
AccII CGCG 1 cut(s) 466
AciI CCGC 6 cut(s) 117, 185, 203, 251, 565, 1477
AclWI GGATC 1 cut(s) 692
AcoI YGGCCR 2 cut(s) 104, 1474
AcsI RAATTY 2 cut(s) 635, 830
AcuI CTGAAG 1 cut(s) 1163
AfaI GTAC 3 cut(s) 368, 1097, 1496
AfiI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
AjiI CACGTC 1 cut(s) 32
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 7 cut(s) 325, 340, 620, 874, 907, 1112, 1355
AluI AGCT 7 cut(s) 325, 340, 620, 874, 907, 1112, 1355
Alw26I GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
AlwI GGATC 1 cut(s) 692
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 6 cut(s) 104, 186, 253, 1156, 1292, 1474
ApoI RAATTY 2 cut(s) 635, 830
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
AseI ATTAAT 1 cut(s) 1043
Asp700I GAANNNNTTC 1 cut(s) 1341
AspLEI GCGC 2 cut(s) 468, 1249
AspS9I GGNCC 3 cut(s) 187, 253, 1157
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 484, 605
AsuNHI GCTAGC 1 cut(s) 907
AvaI CYCGRG 1 cut(s) 326
BanII GRGCYC 2 cut(s) 378, 750
BarI GAAGNNNNNNTAC 2 cut(s) 677, 709
BauI CACGAG 3 cut(s) 22, 470, 1229
BbsI GAAGAC 1 cut(s) 405
BbvCI CCTCAGC 1 cut(s) 621
BceAI ACGGC 1 cut(s) 410
BciT130I CCWGG 1 cut(s) 257
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BfaI CTAG 4 cut(s) 908, 950, 971, 1305
BfmI CTRYAG 1 cut(s) 1018
BisI GCNGC 2 cut(s) 186, 1477
BlsI GCNGC 2 cut(s) 187, 1478
Bme1390I CCNGG 2 cut(s) 88, 257
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 187, 253, 1157
BmiI GGNNCC 2 cut(s) 375, 747
BmrFI CCNGG 2 cut(s) 88, 257
BmsI GCATC 3 cut(s) 60, 412, 505
BmtI GCTAGC 1 cut(s) 911
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 997, 1029
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 2 cut(s) 326, 1375
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 2 cut(s) 960, 1003
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 6 cut(s) 127, 176, 534, 639, 726, 1462
BseLI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BseMII CTCAG 3 cut(s) 492, 612, 765
BseRI GAGGAG 4 cut(s) 386, 488, 1021, 1247
BseYI CCCAGC 3 cut(s) 62, 773, 1159
BsgI GTGCAG 2 cut(s) 698, 1281
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 6 cut(s) 106, 188, 255, 1158, 1294, 1476
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 2 cut(s) 88, 351
BslFI GGGAC 2 cut(s) 70, 1151
BslI CCNNNNNNNGG 4 cut(s) 87, 202, 582, 976
BsmAI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BsmBI CGTCTC 2 cut(s) 38, 614
BsmFI GGGAC 2 cut(s) 70, 1151
BsnI GGCC 6 cut(s) 106, 188, 255, 1158, 1294, 1476
Bso31I GGTCTC 2 cut(s) 960, 1003
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 378, 750
Bsp143I GATC 3 cut(s) 697, 919, 960
BspACI CCGC 6 cut(s) 117, 185, 203, 251, 565, 1477
BspANI GGCC 6 cut(s) 106, 188, 255, 1158, 1294, 1476
BspCNI CTCAG 3 cut(s) 493, 613, 764
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 375, 747
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 1 cut(s) 692
BspQI GCTCTTC 1 cut(s) 1182
BspTNI GGTCTC 2 cut(s) 960, 1003
BsrBI CCGCTC 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 3 cut(s) 697, 919, 960
BssSI CACGAG 3 cut(s) 22, 470, 1229
Bst2BI CACGAG 3 cut(s) 22, 470, 1229
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 6 cut(s) 172, 608, 864, 1333, 1411, 1483
Bst6I CTCTTC 8 cut(s) 239, 282, 428, 894, 951, 998, 1182, 1427
BstC8I GCNNGC 4 cut(s) 253, 909, 1251, 1296
BstDEI CTNAG 5 cut(s) 501, 621, 751, 1351, 1377
BstENI CCTNNNNNAGG 1 cut(s) 974
BstF5I GGATG 6 cut(s) 127, 176, 534, 639, 726, 1462
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 2 cut(s) 468, 1249
BstKTI GATC 3 cut(s) 700, 922, 963
BstMAI GTCTC 6 cut(s) 38, 348, 497, 614, 960, 1003
BstMBI GATC 3 cut(s) 697, 919, 960
BstMWI GCNNNNNNNGC 1 cut(s) 1109
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 2 cut(s) 773, 1253
BstSCI CCNGG 2 cut(s) 86, 255
BstSFI CTRYAG 1 cut(s) 1018
BstUI CGCG 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 405
BstXI CCANNNNNNTGG 1 cut(s) 138
BsuRI GGCC 6 cut(s) 106, 188, 255, 1158, 1294, 1476
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 6 cut(s) 127, 176, 534, 639, 726, 1462
BtsI GCAGTG 2 cut(s) 686, 1098
BtsIMutI CAGTG 4 cut(s) 177, 686, 1098, 1479
Cac8I GCNNGC 4 cut(s) 253, 909, 1251, 1296
CfoI GCGC 2 cut(s) 468, 1249
Cfr13I GGNCC 3 cut(s) 187, 253, 1157
Csp6I GTAC 3 cut(s) 367, 1096, 1495
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 6 cut(s) 298, 734, 770, 841, 1127, 1250
CviQI GTAC 3 cut(s) 367, 1096, 1495
DdeI CTNAG 5 cut(s) 501, 621, 751, 1351, 1377
DpnI GATC 3 cut(s) 699, 921, 962
DpnII GATC 3 cut(s) 697, 919, 960
EaeI YGGCCR 2 cut(s) 104, 1474
Eam1104I CTCTTC 8 cut(s) 239, 282, 428, 894, 951, 998, 1182, 1427
EarI CTCTTC 8 cut(s) 239, 282, 428, 894, 951, 998, 1182, 1427
EciI GGCGGA 2 cut(s) 218, 554
Eco24I GRGCYC 2 cut(s) 378, 750
Eco31I GGTCTC 2 cut(s) 960, 1003
Eco57I CTGAAG 1 cut(s) 1163
Eco88I CYCGRG 1 cut(s) 326
EcoNI CCTNNNNNAGG 1 cut(s) 974
EcoRI GAATTC 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 255
EcoT38I GRGCYC 2 cut(s) 378, 750
Esp3I CGTCTC 2 cut(s) 38, 614
FaeI CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
FalI AAGNNNNNCTT 4 cut(s) 1384, 1416, 1406, 1438
FaqI GGGAC 2 cut(s) 70, 1151
FatI CATG 6 cut(s) 297, 733, 769, 840, 1126, 1249
FauI CCCGC 2 cut(s) 124, 244
FauNDI CATATG 1 cut(s) 1189
Fnu4HI GCNGC 2 cut(s) 186, 1477
FokI GGATG 6 cut(s) 114, 163, 541, 626, 713, 1469
FriOI GRGCYC 2 cut(s) 378, 750
Fsp4HI GCNGC 2 cut(s) 186, 1477
FspBI CTAG 4 cut(s) 908, 950, 971, 1305
FspI TGCGCA 1 cut(s) 1248
GlaI GCGC 2 cut(s) 467, 1248
GluI GCNGC 2 cut(s) 186, 1477
GsaI CCCAGC 3 cut(s) 66, 777, 1163
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 6 cut(s) 106, 188, 255, 1158, 1294, 1476
HapII CCGG 2 cut(s) 88, 351
HhaI GCGC 2 cut(s) 468, 1249
Hin1II CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
Hin6I GCGC 2 cut(s) 466, 1247
HinP1I GCGC 2 cut(s) 466, 1247
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 5 cut(s) 58, 383, 723, 1207, 1396
HpaII CCGG 2 cut(s) 88, 351
HphI GGTGA 2 cut(s) 484, 605
Hpy166II GTNNAC 4 cut(s) 414, 462, 1054, 1098
Hpy188I TCNGA 5 cut(s) 388, 453, 965, 1182, 1206
Hpy188III TCNNGA 8 cut(s) 40, 55, 180, 328, 419, 430, 950, 1463
Hpy8I GTNNAC 4 cut(s) 414, 462, 1054, 1098
Hpy99I CGWCG 3 cut(s) 398, 821, 824
HpyAV CCTTC 7 cut(s) 206, 325, 578, 679, 838, 931, 1177
HpyCH4III ACNGT 6 cut(s) 172, 608, 864, 1333, 1411, 1483
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 679, 1253, 1298
HpyF10VI GCNNNNNNNGC 1 cut(s) 1109
HpyF3I CTNAG 5 cut(s) 501, 621, 751, 1351, 1377
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
HspAI GCGC 2 cut(s) 466, 1247
Kzo9I GATC 3 cut(s) 697, 919, 960
LguI GCTCTTC 1 cut(s) 1182
LmnI GCTCC 3 cut(s) 373, 745, 1234
LweI GCATC 3 cut(s) 60, 412, 505
MaeI CTAG 4 cut(s) 908, 950, 971, 1305
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 2 cut(s) 27, 1069
MalI GATC 3 cut(s) 699, 921, 962
MbiI CCGCTC 1 cut(s) 185
MboI GATC 3 cut(s) 697, 919, 960
MfeI CAATTG 1 cut(s) 1242
MhlI GDGCHC 2 cut(s) 378, 750
MluCI AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1242
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 1 cut(s) 754
MroXI GAANNNNTTC 1 cut(s) 1341
MseI TTAA 5 cut(s) 876, 1038, 1043, 1371, 1434
MslI CAYNNNNRTG 1 cut(s) 136
MspI CCGG 2 cut(s) 88, 351
MspR9I CCNGG 2 cut(s) 88, 257
MunI CAATTG 1 cut(s) 1242
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 1 cut(s) 1109
NciI CCSGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 1189
NdeII GATC 3 cut(s) 697, 919, 960
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 6 cut(s) 301, 737, 773, 844, 1130, 1253
NlaIV GGNNCC 2 cut(s) 375, 747
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 27
NsbI TGCGCA 1 cut(s) 1248
NspI RCATGY 2 cut(s) 773, 1253
PaeI GCATGC 1 cut(s) 1253
PaeR7I CTCGAG 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 1182
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 4 cut(s) 58, 383, 1207, 1396
PfoI TCCNGGA 1 cut(s) 86
PkrI GCNGC 2 cut(s) 187, 1478
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PshBI ATTAAT 1 cut(s) 1043
PsiI TTATAA 3 cut(s) 522, 1269, 1404
Psp6I CCWGG 1 cut(s) 255
PspFI CCCAGC 3 cut(s) 62, 773, 1159
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 2 cut(s) 375, 747
PspPI GGNCC 3 cut(s) 187, 253, 1157
RsaI GTAC 3 cut(s) 368, 1097, 1496
RsaNI GTAC 3 cut(s) 367, 1096, 1495
RseI CAYNNNNRTG 1 cut(s) 136
SapI GCTCTTC 1 cut(s) 1182
SaqAI TTAA 5 cut(s) 876, 1038, 1043, 1371, 1434
SatI GCNGC 2 cut(s) 186, 1477
Sau3AI GATC 3 cut(s) 697, 919, 960
Sau96I GGNCC 3 cut(s) 187, 253, 1157
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 2 cut(s) 88, 257
SduI GDGCHC 2 cut(s) 378, 750
SfaNI GCATC 3 cut(s) 60, 412, 505
SfcI CTRYAG 1 cut(s) 1018
Sfr274I CTCGAG 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 136
SmlI CTYRAG 3 cut(s) 326, 341, 1390
SmoI CTYRAG 3 cut(s) 326, 341, 1390
SphI GCATGC 1 cut(s) 1253
Sse9I AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1242
SsiI CCGC 6 cut(s) 117, 185, 203, 251, 565, 1477
SspMI CTAG 4 cut(s) 908, 950, 971, 1305
StyD4I CCNGG 2 cut(s) 86, 255
TaaI ACNGT 6 cut(s) 172, 608, 864, 1333, 1411, 1483
TaiI ACGT 1 cut(s) 34
TaqI TCGA 3 cut(s) 327, 897, 1450
TaqII GACCGA 1 cut(s) 291
TasI AATT 8 cut(s) 537, 635, 715, 795, 830, 1040, 1120, 1242
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 2 cut(s) 188, 1479
TfiI GAWTC 4 cut(s) 58, 383, 1207, 1396
Tru1I TTAA 5 cut(s) 876, 1038, 1043, 1371, 1434
Tru9I TTAA 5 cut(s) 876, 1038, 1043, 1371, 1434
TscAI CASTG 4 cut(s) 177, 686, 1105, 1486
TseFI GTSAC 1 cut(s) 27
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 8 cut(s) 300, 420, 545, 617, 628, 750, 857, 1331
TspRI CASTG 4 cut(s) 177, 686, 1105, 1486
VspI ATTAAT 1 cut(s) 1043
XagI CCTNNNNNAGG 1 cut(s) 974
XapI RAATTY 2 cut(s) 635, 830
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 2 cut(s) 773, 1253
XhoI CTCGAG 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 4 cut(s) 908, 950, 971, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.