AT3G44060

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000813
Physical Location & Seq
Reverse (-)
15824938 .. 15826391
1454 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G44060.1

Sequence Viewer

Length: 1284 bp
ATGGATTGTCTTCCAGATGATCTCCTTGTCCAAATCTTATATTTACTTCCCACGAAAGAGGCTGTTTCAACCTCTGTTCTCTCCAAGAGATGGAGGACTCTGTTTACTCGCAGTGATAATCTTGACTTTCACGATCCCATCTCCGGGCGCCCCGAAGATATCCTAAAGAGTTTCAATGATTTCGTGGACAGTTCATTAGCTTTTCAAGGTGGTAAACATATAAAAAAGTTCTCACTTCATACTAAAATTAAAACATTTGAATATCACGTACTTGACCGCTGGATTTGTAATGCCCTGGAACACGGTGTCTCTGAACTTCATCTACACCTAATGCATGAATCGTGGCCGTGGTTGTTTTCTATCCCATCTAAAGTTTTCAACAGCTCAACACTGGTTAAGCTGTCATTGGGATCGAGACTATACTGCCCAAGTTTTCCTCCGGATACATCTCTCCCAGCACTAAAGGTTCTGCTCCTTGATTCAATCTTGTTTCGGGATGATCAATTATCTAATGTGTTTCTCGCTGCATGCCCTGCACTTGAGGACTTAACCATACATCACACGTATCACCCATGCGTCATATCCAGCAAAAGCATTAAGAAACTTTCACTTAGCGTTAACAGTGGTTATTATGGTGCTGGTTATATTCTTACACTTGACACGCCAAGTGTTGTCGACCTCTACTACTCTGATTCCCCTCGGCATAACGCTCCACTTTTTCATTTAGATTCACTTGCGAAAGTTACATTGGACCTTCATTTCATAGAAAATAACAATCGCGAGGTGCAAAATGACGCTGATGTGAAAAATCTCATTAGGGAGATATGCAACGTCAAGACCCTTCACTTGACTTGTTCTACAGTCGAGGTGATTTCCGTATACTGCAAAGGTGGACTACCAATGTTCAACAACCTTGTTGAGTTAGTGTTTTCGAGTAAAAAGGAAGGTTGGAGAGTGCTTCTGCCTCTTCTGCTAGAGAATTCTCCAAACCTTGAAACTCTGGTTCTCTCGGATCTGCATCGTTACACATTTGGACGAAGACATCGGTTTGTAGGGATTCCAATTCCCCCGAATAACCAAATAAAGGTACTGCGTATCATGCAGTACCAAGGAAGTGCAACTGTACTGAAACACATTAGTCATTTCTTACTGAATATGGATTGTCTTGAAGTTATGAAAGTCAACGTTGCAGCTGCATTGGATGACCCCAAAAAGATGCAGCTCACAGAGGATCTGTTAAAGCTTCCCACAGCTTCATGCAAGCTCAAGATTCAAGTCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

48.44

Weight (kDa)

7.29

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 40 2.9e-08 F-box domain
LRR_At1g61320_AtMIF1 PF23622 71 - 398 2e-08 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 91 - 284 3.7e-15 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 90
AccI GTMKAC 2 cut(s) 675, 879
AccII CGCG 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 1 cut(s) 277
AclI AACGTT 1 cut(s) 1185
AclWI GGATC 4 cut(s) 128, 418, 1020, 1239
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 1 cut(s) 979
AcyI GRCGYC 1 cut(s) 148
AfaI GTAC 4 cut(s) 270, 1089, 1106, 1125
AfiI CCNNNNNNNGG 4 cut(s) 90, 143, 144, 1084
AflIII ACRYGT 1 cut(s) 561
AjnI CCWGG 1 cut(s) 294
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AluBI AGCT 8 cut(s) 200, 384, 400, 1193, 1222, 1243, 1253, 1264
AluI AGCT 8 cut(s) 200, 384, 400, 1193, 1222, 1243, 1253, 1264
Alw26I GTCTC 2 cut(s) 313, 409
AlwI GGATC 4 cut(s) 128, 418, 1020, 1239
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 524, 1190, 1193, 1219
ApoI RAATTY 1 cut(s) 979
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 560, 880
AvaII GGWCC 1 cut(s) 751
BanI GGYRCC 1 cut(s) 147
BbsI GAAGAC 2 cut(s) 2, 1045
BbvI GCAGC 4 cut(s) 511, 1180, 1202, 1231
BccI CCATC 3 cut(s) 84, 146, 373
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 296
BciVI GTATCC 1 cut(s) 436
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 1 cut(s) 145
BcoDI GTCTC 2 cut(s) 313, 409
BfaI CTAG 1 cut(s) 974
BfmI CTRYAG 1 cut(s) 858
BfoI RGCGCY 1 cut(s) 151
BfuI GTATCC 1 cut(s) 436
BisI GCNGC 4 cut(s) 525, 1191, 1194, 1220
BlsI GCNGC 4 cut(s) 526, 1192, 1195, 1221
Bme1390I CCNGG 2 cut(s) 145, 296
Bme18I GGWCC 1 cut(s) 751
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 145, 296
BmsI GCATC 2 cut(s) 1027, 1206
BpiI GAAGAC 2 cut(s) 2, 1045
BpuEI CTTGAG 2 cut(s) 560, 1250
BpuMI CCSGG 1 cut(s) 145
BsaAI YACGTR 2 cut(s) 268, 564
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 1 cut(s) 148
BsaJI CCNNGG 4 cut(s) 294, 347, 698, 1108
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 143, 144, 1084
Bse1I ACTGG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 1017
BseAI TCCGGA 1 cut(s) 439
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 4 cut(s) 294, 347, 698, 1108
BseGI GGATG 2 cut(s) 502, 1207
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 4 cut(s) 90, 143, 144, 1084
BseNI ACTGG 1 cut(s) 396
BseXI GCAGC 4 cut(s) 511, 1180, 1202, 1231
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 780
BshFI GGCC 1 cut(s) 346
BshNI GGYRCC 1 cut(s) 147
BsiSI CCGG 2 cut(s) 144, 440
BslI CCNNNNNNNGG 4 cut(s) 90, 143, 144, 1084
BsmAI GTCTC 2 cut(s) 313, 409
BsnI GGCC 1 cut(s) 346
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
Bsp68I TCGCGA 1 cut(s) 780
BspACI CCGC 1 cut(s) 277
BspANI GGCC 1 cut(s) 346
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 1 cut(s) 149
BspPI GGATC 4 cut(s) 128, 418, 1020, 1239
BspT107I GGYRCC 1 cut(s) 147
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 4 cut(s) 294, 347, 698, 1108
BssMI GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
BssNAI GTATAC 1 cut(s) 880
BssNI GRCGYC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 1108
Bst1107I GTATAC 1 cut(s) 880
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 5 cut(s) 191, 305, 623, 862, 1123
Bst6I CTCTTC 1 cut(s) 972
BstACI GRCGYC 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBAI YACGTR 2 cut(s) 268, 564
BstC8I GCNNGC 2 cut(s) 529, 1262
BstDEI CTNAG 1 cut(s) 611
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 2 cut(s) 502, 1207
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 1 cut(s) 150
BstKTI GATC 6 cut(s) 22, 136, 413, 502, 1015, 1234
BstMAI GTCTC 2 cut(s) 313, 409
BstMBI GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
BstMWI GCNNNNNNNGC 3 cut(s) 533, 970, 1099
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 531
BstSCI CCNGG 2 cut(s) 143, 294
BstSFI CTRYAG 1 cut(s) 858
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 4 cut(s) 511, 1180, 1202, 1231
BstV2I GAAGAC 2 cut(s) 2, 1045
BstX2I RGATCY 2 cut(s) 1012, 1231
BstYI RGATCY 2 cut(s) 1012, 1231
BstZ17I GTATAC 1 cut(s) 880
BsuI GTATCC 1 cut(s) 436
BsuRI GGCC 1 cut(s) 346
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 2 cut(s) 502, 1207
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 3 cut(s) 118, 389, 628
BtuMI TCGCGA 1 cut(s) 780
Cac8I GCNNGC 2 cut(s) 529, 1262
CfoI GCGC 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 751
CseI GACGC 2 cut(s) 565, 803
Csp6I GTAC 4 cut(s) 269, 1088, 1105, 1124
CviAII CATG 5 cut(s) 335, 528, 573, 1099, 1257
CviQI GTAC 4 cut(s) 269, 1088, 1105, 1124
DdeI CTNAG 1 cut(s) 611
DinI GGCGCC 1 cut(s) 149
DpnI GATC 6 cut(s) 21, 135, 412, 501, 1014, 1233
DpnII GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Eco130I CCWWGG 1 cut(s) 1108
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 979
EcoRII CCWGG 1 cut(s) 294
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 1108
EcoT22I ATGCAT 1 cut(s) 336
EgeI GGCGCC 1 cut(s) 149
EheI GGCGCC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 1108
FaeI CATG 5 cut(s) 338, 531, 576, 1102, 1260
FatI CATG 5 cut(s) 334, 527, 572, 1098, 1256
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 2 cut(s) 675, 879
Fnu4HI GCNGC 4 cut(s) 525, 1191, 1194, 1220
FokI GGATG 2 cut(s) 509, 1214
Fsp4HI GCNGC 4 cut(s) 525, 1191, 1194, 1220
FspBI CTAG 1 cut(s) 974
GlaI GCGC 1 cut(s) 149
GluI GCNGC 4 cut(s) 525, 1191, 1194, 1220
GsaI CCCAGC 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 1 cut(s) 346
HapII CCGG 2 cut(s) 144, 440
HgaI GACGC 2 cut(s) 565, 803
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 5 cut(s) 338, 531, 576, 1102, 1260
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HincII GTYRAC 3 cut(s) 619, 676, 1183
HindII GTYRAC 3 cut(s) 619, 676, 1183
HindIII AAGCTT 1 cut(s) 1241
HinfI GANTC 7 cut(s) 97, 338, 479, 692, 728, 1057, 1270
HpaI GTTAAC 1 cut(s) 619
HpaII CCGG 2 cut(s) 144, 440
HphI GGTGA 2 cut(s) 560, 880
Hpy166II GTNNAC 8 cut(s) 105, 187, 215, 619, 676, 880, 893, 1183
Hpy188I TCNGA 3 cut(s) 313, 691, 1012
Hpy8I GTNNAC 8 cut(s) 105, 187, 215, 619, 676, 880, 893, 1183
HpyAV CCTTC 3 cut(s) 764, 851, 938
HpyCH4III ACNGT 5 cut(s) 191, 305, 623, 862, 1123
HpyCH4IV ACGT 4 cut(s) 267, 563, 831, 1185
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 970, 1099
HpyF3I CTNAG 1 cut(s) 611
HpySE526I ACGT 4 cut(s) 267, 563, 831, 1185
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 5 cut(s) 338, 531, 576, 1102, 1260
HspAI GCGC 1 cut(s) 148
KasI GGCGCC 1 cut(s) 147
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 1 cut(s) 499
KspAI GTTAAC 1 cut(s) 619
Kzo9I GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
LmnI GCTCC 2 cut(s) 477, 715
Lsp1109I GCAGC 4 cut(s) 511, 1180, 1202, 1231
LweI GCATC 2 cut(s) 1027, 1206
MaeI CTAG 1 cut(s) 974
MaeII ACGT 4 cut(s) 267, 563, 831, 1185
MaeIII GTNAC 2 cut(s) 742, 1022
MalI GATC 6 cut(s) 21, 135, 412, 501, 1014, 1233
MboI GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
MboII GAAGA 3 cut(s) 167, 959, 1050
MflI RGATCY 2 cut(s) 1012, 1231
MluCI AATT 4 cut(s) 246, 503, 979, 1062
Mly113I GGCGCC 1 cut(s) 148
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 929
Mph1103I ATGCAT 1 cut(s) 336
MroI TCCGGA 1 cut(s) 439
MseI TTAA 6 cut(s) 249, 396, 548, 597, 618, 1238
MspA1I CMGCKG 2 cut(s) 279, 1193
MspI CCGG 2 cut(s) 144, 440
MspR9I CCNGG 2 cut(s) 145, 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 3 cut(s) 533, 970, 1099
NarI GGCGCC 1 cut(s) 148
NciI CCSGG 1 cut(s) 145
NdeII GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
NlaIII CATG 5 cut(s) 338, 531, 576, 1102, 1260
NlaIV GGNNCC 1 cut(s) 149
NmeAIII GCCGAG 1 cut(s) 679
NruI TCGCGA 1 cut(s) 780
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 1 cut(s) 531
PaeI GCATGC 1 cut(s) 531
PfeI GAWTC 6 cut(s) 338, 479, 692, 728, 1057, 1270
PflMI CCANNNNNTGG 1 cut(s) 90
PkrI GCNGC 4 cut(s) 526, 1192, 1195, 1221
PleI GAGTC 1 cut(s) 91
PluTI GGCGCC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 91
Ppu21I YACGTR 2 cut(s) 268, 564
Psp1406I AACGTT 1 cut(s) 1185
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 751
PsrI GAACNNNNNNTAC 2 cut(s) 306, 338
PsuI RGATCY 2 cut(s) 1012, 1231
PvuII CAGCTG 1 cut(s) 1193
RruI TCGCGA 1 cut(s) 780
RsaI GTAC 4 cut(s) 270, 1089, 1106, 1125
RsaNI GTAC 4 cut(s) 269, 1088, 1105, 1124
SalI GTCGAC 1 cut(s) 674
SaqAI TTAA 6 cut(s) 249, 396, 548, 597, 618, 1238
SatI GCNGC 4 cut(s) 525, 1191, 1194, 1220
Sau3AI GATC 6 cut(s) 19, 133, 410, 499, 1012, 1231
Sau96I GGNCC 1 cut(s) 751
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 145, 296
SfaNI GCATC 2 cut(s) 1027, 1206
SfcI CTRYAG 1 cut(s) 858
SfoI GGCGCC 1 cut(s) 149
SinI GGWCC 1 cut(s) 751
SmlI CTYRAG 2 cut(s) 539, 1265
SmoI CTYRAG 2 cut(s) 539, 1265
SphI GCATGC 1 cut(s) 531
Sse9I AATT 4 cut(s) 246, 503, 979, 1062
SsiI CCGC 1 cut(s) 277
SspDI GGCGCC 1 cut(s) 147
SspMI CTAG 1 cut(s) 974
StyD4I CCNGG 2 cut(s) 143, 294
StyI CCWWGG 1 cut(s) 1108
TaaI ACNGT 5 cut(s) 191, 305, 623, 862, 1123
TaiI ACGT 4 cut(s) 270, 566, 834, 1188
TaqI TCGA 4 cut(s) 413, 675, 864, 932
TasI AATT 4 cut(s) 246, 503, 979, 1062
TatI WGTACW 1 cut(s) 1123
TfiI GAWTC 6 cut(s) 338, 479, 692, 728, 1057, 1270
Tru1I TTAA 6 cut(s) 249, 396, 548, 597, 618, 1238
Tru9I TTAA 6 cut(s) 249, 396, 548, 597, 618, 1238
TscAI CASTG 3 cut(s) 118, 396, 628
TseI GCWGC 4 cut(s) 524, 1190, 1193, 1219
TspDTI ATGAA 9 cut(s) 183, 227, 308, 351, 710, 746, 751, 1190, 1245
TspGWI ACGGA 1 cut(s) 865
TspRI CASTG 3 cut(s) 118, 396, 628
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 751
XapI RAATTY 1 cut(s) 979
XceI RCATGY 1 cut(s) 531
XmiI GTMKAC 2 cut(s) 675, 879
XspI CTAG 1 cut(s) 974
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.