Belongs to the SKP1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 166 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AfaI | GTAC | 1 cut(s) | 88 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 166 |
| AgeI | ACCGGT | 1 cut(s) | 49 |
| AgsI | TTSAA | 2 cut(s) | 58, 146 |
| AjiI | CACGTC | 1 cut(s) | 35 |
| AjnI | CCWGG | 1 cut(s) | 159 |
| AluBI | AGCT | 2 cut(s) | 72, 142 |
| AluI | AGCT | 2 cut(s) | 72, 142 |
| Alw26I | GTCTC | 1 cut(s) | 138 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 166 |
| AoxI | GGCC | 1 cut(s) | 3 |
| ApeKI | GCWGC | 1 cut(s) | 112 |
| AsiGI | ACCGGT | 1 cut(s) | 49 |
| AsuHPI | GGTGA | 1 cut(s) | 25 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BbvI | GCAGC | 1 cut(s) | 99 |
| BccI | CCATC | 1 cut(s) | 4 |
| BciT130I | CCWGG | 1 cut(s) | 161 |
| BcoDI | GTCTC | 1 cut(s) | 138 |
| BisI | GCNGC | 1 cut(s) | 113 |
| BlsI | GCNGC | 1 cut(s) | 114 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| BmgBI | CACGTC | 1 cut(s) | 35 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BsaJI | CCNNGG | 1 cut(s) | 160 |
| BsaWI | WCCGGW | 1 cut(s) | 49 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 166 |
| Bse118I | RCCGGY | 1 cut(s) | 49 |
| BseBI | CCWGG | 1 cut(s) | 161 |
| BseDI | CCNNGG | 1 cut(s) | 160 |
| BseGI | GGATG | 1 cut(s) | 156 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 166 |
| BseXI | GCAGC | 1 cut(s) | 99 |
| BshFI | GGCC | 1 cut(s) | 5 |
| BshTI | ACCGGT | 1 cut(s) | 49 |
| BsiSI | CCGG | 1 cut(s) | 50 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 166 |
| BsmAI | GTCTC | 1 cut(s) | 138 |
| BsnI | GGCC | 1 cut(s) | 5 |
| BspANI | GGCC | 1 cut(s) | 5 |
| BsrFI | RCCGGY | 1 cut(s) | 49 |
| BssAI | RCCGGY | 1 cut(s) | 49 |
| BssECI | CCNNGG | 1 cut(s) | 160 |
| Bst2UI | CCWGG | 1 cut(s) | 161 |
| BstF5I | GGATG | 1 cut(s) | 156 |
| BstMAI | GTCTC | 1 cut(s) | 138 |
| BstNI | CCWGG | 1 cut(s) | 161 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstV1I | GCAGC | 1 cut(s) | 99 |
| BsuRI | GGCC | 1 cut(s) | 5 |
| BtrI | CACGTC | 1 cut(s) | 35 |
| BtsCI | GGATG | 1 cut(s) | 156 |
| CaiI | CAGNNNCTG | 1 cut(s) | 166 |
| Cfr10I | RCCGGY | 1 cut(s) | 49 |
| Csp6I | GTAC | 1 cut(s) | 87 |
| CspAI | ACCGGT | 1 cut(s) | 49 |
| CviJI | RGCY | 4 cut(s) | 5, 72, 142, 166 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 72, 142, 166 |
| CviQI | GTAC | 1 cut(s) | 87 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| EcoRII | CCWGG | 1 cut(s) | 159 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 61, 93 |
| Fnu4HI | GCNGC | 1 cut(s) | 113 |
| FokI | GGATG | 1 cut(s) | 143 |
| Fsp4HI | GCNGC | 1 cut(s) | 113 |
| GluI | GCNGC | 1 cut(s) | 113 |
| HaeIII | GGCC | 1 cut(s) | 5 |
| HapII | CCGG | 1 cut(s) | 50 |
| HindIII | AAGCTT | 2 cut(s) | 70, 140 |
| HinfI | GANTC | 1 cut(s) | 16 |
| HpaII | CCGG | 1 cut(s) | 50 |
| HphI | GGTGA | 1 cut(s) | 25 |
| Hpy166II | GTNNAC | 1 cut(s) | 38 |
| Hpy188I | TCNGA | 1 cut(s) | 123 |
| Hpy8I | GTNNAC | 1 cut(s) | 38 |
| HpyCH4IV | ACGT | 1 cut(s) | 34 |
| HpySE526I | ACGT | 1 cut(s) | 34 |
| LpnPI | CCDG | 6 cut(s) | 50, 63, 146, 152, 167, 173 |
| Lsp1109I | GCAGC | 1 cut(s) | 99 |
| MaeII | ACGT | 1 cut(s) | 34 |
| MaeIII | GTNAC | 1 cut(s) | 175 |
| MboII | GAAGA | 1 cut(s) | 11 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MmeI | TCCRAC | 1 cut(s) | 83 |
| MnlI | CCTC | 1 cut(s) | 92 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspI | CCGG | 1 cut(s) | 50 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| MvaI | CCWGG | 1 cut(s) | 161 |
| PfeI | GAWTC | 1 cut(s) | 16 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 166 |
| PinAI | ACCGGT | 1 cut(s) | 49 |
| PkrI | GCNGC | 1 cut(s) | 114 |
| Psp6I | CCWGG | 1 cut(s) | 159 |
| PspGI | CCWGG | 1 cut(s) | 159 |
| PstNI | CAGNNNCTG | 1 cut(s) | 166 |
| RsaI | GTAC | 1 cut(s) | 88 |
| RsaNI | GTAC | 1 cut(s) | 87 |
| SatI | GCNGC | 1 cut(s) | 113 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SetI | ASST | 4 cut(s) | 37, 74, 103, 144 |
| SgeI | CNNG | 9 cut(s) | 19, 47, 62, 77, 85, 155, 172, 173, 179 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| TaiI | ACGT | 1 cut(s) | 37 |
| TfiI | GAWTC | 1 cut(s) | 16 |
| TseI | GCWGC | 1 cut(s) | 112 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 166 |