AT3G59210

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000813
Physical Location & Seq
Forward (+)
21889476 .. 21891944
2469 bp
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UTR
Exon/CDS
Intron
AT3G59210.5

Sequence Viewer

Length: 1455 bp
ATGGATGATTGTTCCAAAGATATAATCAATTGTTTACCAGACAATCTTCTTTGCCAAATCTTGTCCAACTTGTCCACGAAAGAAGCTGCTTTGACATCACTTCTCTCAAAAAGATGGCGGTATTTGTTTGCTCTTGTACCAAATCTTGATTTTGATGTCCTTCCCTCTCTGCATCCCGAGGTTGCGATGCAGGACCAGGATCAGACGAGTTTTATAGATTTTGTGGATAGAGTATTGAAATTGCGAGGGAAAGATCATATTAACAAGTTCTCTCTGAAATGTGGAGATGGTATTGAGGATGAAGATGTCTTCCCTTGGATATTAAATACCTTGAGACATGGTGTATCAGATCTTTCTCTACATGTCTCTCCCTCTTTGGTTTATTGGTTGCCTTCAAAGGTTTTTGCAAGTAAGACACTGGTTAGGCTGAAAATAGGACCTAAAGATGGTCCTAGAGTTAAACTGAGAAATGTCTGTCTACCAAAGCTTAAGACTCTAAATCTTGATTCAGTTGTGTTTGAAGAGGGTAAGATTGGTTTTGCAAAGCTTCTTTCTGGTTGTCCCGTGCTCGAGGAATTATCTTTGCTTAATCTTGCGTGGGATCGTTGGGATTCTTGCTCTGTGTCTTCCAAAATCCTCAAGAGACTAACGCTATATTGTGCACACAGTAGCAGAAATCCAAAGAGTGTATCATTTGATACTCCAAATGTTGTCTACTTTGAATATTCTGATAATATTGCAAACAAGTATCCGAAAGTGAATTTTGATTCGCTGGTTGAAGCTAGTATCGGTATTCGGATGACGAAGGTTCAGAAAGCAAATGCGAGATATGTGTCAGATGTTGATGAGGAAACAGAGATGGTTGGCAATGCAACGGATCTTCTTATGGGAATATGCAATGTGAAGACCCTTTACTTATCTTATGACACTCTTGAGACACTTAATCTTTGCTGTCAAGTGATACCGGTATTCAACAATTTGACTCATCTAACTATTGAGAGTCATCCGGAACTAGGATGGGAATCATTGCCAAATCTTCTCAAGAGTTGTCCAAATCTTGGAACTCTTGTCTTCCAAGGACTCCTTCACAAAGCAACAGATAGATGTGGGGATGTGTGCATGTGCCAAGGTTTGGAGAATAGTCATTCTTGCTTATCATCAAGTCCGGTGAAGTGTCTAAAGATATTGAAATTTGGAGAAACCAATGATGATGACGATATGGAGATAGAGATGGAGCAGATCAAGAATTTTTTGGAGAAAATGCCTAATCTTGAACAACTCATAATATACTACGAATCATCAATCGATAATGATTTAGTTAGAGTATCAAGCCAACTTCAGGAGGTTCCTACAGTAGCTTCACTCAAGTGCAAGATTCAAGTAATCTCTGATAACCTTAGCTTGTCATCCACTTTGCCCATTTCCTTGTCGATGGAAAATGGATTTCACCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

484

Amino Acids

54.35

Weight (kDa)

5.26

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 9 - 45 5.1e-08 F-box domain
LRR_At5g56370 PF24758 106 - 221 3.4e-12 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 162 - 188 2e-07 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1058, 1132
AccI GTMKAC 2 cut(s) 478, 714
AccIII TCCGGA 1 cut(s) 1006
AciI CCGC 1 cut(s) 118
AclWI GGATC 3 cut(s) 207, 609, 885
AcsI RAATTY 3 cut(s) 760, 1190, 1246
AcuI CTGAAG 1 cut(s) 1322
AfaI GTAC 1 cut(s) 138
AfiI CCNNNNNNNGG 5 cut(s) 446, 1013, 1058, 1132, 1339
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 361
AgeI ACCGGT 1 cut(s) 964
AgsI TTSAA 9 cut(s) 238, 396, 521, 722, 779, 973, 1189, 1274, 1379
AjnI CCWGG 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 1068, 1100
AleI CACNNNNGTG 1 cut(s) 1366
AluBI AGCT 6 cut(s) 86, 487, 547, 782, 1358, 1401
AluI AGCT 6 cut(s) 86, 487, 547, 782, 1358, 1401
Alw21I GWGCWC 2 cut(s) 570, 664
Alw26I GTCTC 4 cut(s) 328, 370, 637, 929
Alw44I GTGCAC 1 cut(s) 660
AlwI GGATC 3 cut(s) 207, 609, 885
Ama87I CYCGRG 2 cut(s) 176, 569
Aor13HI TCCGGA 1 cut(s) 1006
ApaLI GTGCAC 1 cut(s) 660
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 3 cut(s) 760, 1190, 1246
AsiGI ACCGGT 1 cut(s) 964
AspS9I GGNCC 3 cut(s) 193, 437, 449
AsuHPI GGTGA 2 cut(s) 1180, 1439
AvaI CYCGRG 2 cut(s) 176, 569
AvaII GGWCC 3 cut(s) 193, 437, 449
BaeGI GKGCMC 1 cut(s) 664
BarI GAAGNNNNNNTAC 2 cut(s) 896, 928
BbsI GAAGAC 4 cut(s) 301, 618, 911, 1063
Bbv12I GWGCWC 2 cut(s) 570, 664
BbvI GCAGC 1 cut(s) 73
BccI CCATC 7 cut(s) 108, 281, 440, 853, 1011, 1225, 1426
BciT130I CCWGG 1 cut(s) 197
BciVI GTATCC 1 cut(s) 759
BcoDI GTCTC 4 cut(s) 328, 370, 637, 929
BfaI CTAG 3 cut(s) 453, 783, 1013
BfmI CTRYAG 2 cut(s) 1350, 1451
BfrI CTTAAG 1 cut(s) 488
BfuI GTATCC 1 cut(s) 759
BglII AGATCT 1 cut(s) 349
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 3 cut(s) 193, 437, 449
BmeT110I CYCGRG 2 cut(s) 176, 569
BmgT120I GGNCC 3 cut(s) 193, 437, 449
BmiI GGNNCC 1 cut(s) 1347
BmrFI CCNGG 1 cut(s) 197
BmsI GCATC 2 cut(s) 177, 181
BpiI GAAGAC 4 cut(s) 301, 618, 911, 1063
Bpu10I CCTNAGC 1 cut(s) 1397
BpuEI CTTGAG 5 cut(s) 352, 623, 953, 1025, 1349
Bsa29I ATCGAT 1 cut(s) 1305
BsaBI GATNNNNATC 1 cut(s) 1021
BsaJI CCNNGG 4 cut(s) 177, 314, 1075, 1126
BsaWI WCCGGW 3 cut(s) 964, 1006, 1165
Bsc4I CCNNNNNNNGG 5 cut(s) 446, 1013, 1058, 1132, 1339
Bse118I RCCGGY 1 cut(s) 964
Bse1I ACTGG 1 cut(s) 423
Bse3DI GCAATG 3 cut(s) 874, 904, 1025
Bse8I GATNNNNATC 1 cut(s) 1021
BseAI TCCGGA 1 cut(s) 1006
BseBI CCWGG 1 cut(s) 197
BseCI ATCGAT 1 cut(s) 1305
BseDI CCNNGG 4 cut(s) 177, 314, 1075, 1126
BseGI GGATG 8 cut(s) 10, 172, 304, 804, 1003, 1022, 1117, 1406
BseJI GATNNNNATC 1 cut(s) 1021
BseLI CCNNNNNNNGG 5 cut(s) 446, 1013, 1058, 1132, 1339
BseMI GCAATG 3 cut(s) 874, 904, 1025
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 664
BseXI GCAGC 1 cut(s) 73
BshTI ACCGGT 1 cut(s) 964
BshVI ATCGAT 1 cut(s) 1305
BsiHKAI GWGCWC 2 cut(s) 570, 664
BsiHKCI CYCGRG 2 cut(s) 176, 569
BsiSI CCGG 3 cut(s) 965, 1007, 1166
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 5 cut(s) 446, 1013, 1058, 1132, 1339
BsmAI GTCTC 4 cut(s) 328, 370, 637, 929
BsmFI GGGAC 1 cut(s) 546
BsoBI CYCGRG 2 cut(s) 176, 569
Bsp1286I GDGCHC 2 cut(s) 570, 664
Bsp13I TCCGGA 1 cut(s) 1006
Bsp143I GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
BspACI CCGC 1 cut(s) 118
BspCNI CTCAG 1 cut(s) 456
BspDI ATCGAT 1 cut(s) 1305
BspEI TCCGGA 1 cut(s) 1006
BspLI GGNNCC 1 cut(s) 1347
BspPI GGATC 3 cut(s) 207, 609, 885
BspTI CTTAAG 1 cut(s) 488
BsrDI GCAATG 3 cut(s) 874, 904, 1025
BsrFI RCCGGY 1 cut(s) 964
BsrI ACTGG 1 cut(s) 423
BssAI RCCGGY 1 cut(s) 964
BssECI CCNNGG 4 cut(s) 177, 314, 1075, 1126
BssMI GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
BssT1I CCWWGG 3 cut(s) 314, 1075, 1126
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 668, 1354
Bst6I CTCTTC 1 cut(s) 516
BstAFI CTTAAG 1 cut(s) 488
BstDEI CTNAG 2 cut(s) 464, 1397
BstF5I GGATG 8 cut(s) 10, 172, 304, 804, 1003, 1022, 1117, 1406
BstKTI GATC 6 cut(s) 202, 256, 352, 604, 880, 1242
BstMAI GTCTC 4 cut(s) 328, 370, 637, 929
BstMBI GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 2 cut(s) 365, 1123
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 2 cut(s) 1350, 1451
BstSLI GKGCMC 1 cut(s) 664
BstV1I GCAGC 1 cut(s) 73
BstV2I GAAGAC 4 cut(s) 301, 618, 911, 1063
BstX2I RGATCY 2 cut(s) 349, 877
BstYI RGATCY 2 cut(s) 349, 877
Bsu15I ATCGAT 1 cut(s) 1305
BsuI GTATCC 1 cut(s) 759
BsuTUI ATCGAT 1 cut(s) 1305
BtgZI GCGATG 1 cut(s) 200
BtsCI GGATG 8 cut(s) 10, 172, 304, 804, 1003, 1022, 1117, 1406
BtsIMutI CAGTG 1 cut(s) 416
Cfr10I RCCGGY 1 cut(s) 964
Cfr13I GGNCC 3 cut(s) 193, 437, 449
ClaI ATCGAT 1 cut(s) 1305
Csp6I GTAC 1 cut(s) 137
CspAI ACCGGT 1 cut(s) 964
CviAII CATG 3 cut(s) 338, 362, 1120
CviJI RGCY 8 cut(s) 86, 427, 487, 547, 782, 1332, 1358, 1401
CviKI_1 RGCY 8 cut(s) 86, 427, 487, 547, 782, 1332, 1358, 1401
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 2 cut(s) 464, 1397
DpnI GATC 6 cut(s) 201, 255, 351, 603, 879, 1241
DpnII GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 3 cut(s) 314, 1075, 1126
Eco47I GGWCC 3 cut(s) 193, 437, 449
Eco57I CTGAAG 1 cut(s) 1322
Eco88I CYCGRG 2 cut(s) 176, 569
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 3 cut(s) 314, 1075, 1126
ErhI CCWWGG 3 cut(s) 314, 1075, 1126
FaeI CATG 3 cut(s) 341, 365, 1123
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 1 cut(s) 546
FatI CATG 3 cut(s) 337, 361, 1119
FblI GTMKAC 2 cut(s) 478, 714
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 8 cut(s) 17, 159, 311, 811, 990, 1029, 1124, 1393
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 3 cut(s) 453, 783, 1013
GluI GCNGC 1 cut(s) 87
HapII CCGG 3 cut(s) 965, 1007, 1166
Hin1II CATG 3 cut(s) 341, 365, 1123
HindIII AAGCTT 2 cut(s) 485, 545
HpaII CCGG 3 cut(s) 965, 1007, 1166
HphI GGTGA 2 cut(s) 1180, 1439
Hpy166II GTNNAC 5 cut(s) 35, 75, 479, 662, 715
Hpy188I TCNGA 9 cut(s) 204, 276, 349, 730, 753, 798, 813, 838, 1390
Hpy8I GTNNAC 5 cut(s) 35, 75, 479, 662, 715
HpyAV CCTTC 4 cut(s) 170, 402, 799, 1094
HpyCH4III ACNGT 2 cut(s) 668, 1354
HpyF3I CTNAG 2 cut(s) 464, 1397
Hsp92II CATG 3 cut(s) 341, 365, 1123
Kpn2I TCCGGA 1 cut(s) 1006
Kzo9I GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
LmnI GCTCC 1 cut(s) 1234
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 2 cut(s) 177, 181
MaeI CTAG 3 cut(s) 453, 783, 1013
MalI GATC 6 cut(s) 201, 255, 351, 603, 879, 1241
MboI GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
MboII GAAGA 9 cut(s) 38, 301, 314, 533, 618, 872, 916, 1028, 1063
MfeI CAATTG 1 cut(s) 28
MflI RGATCY 2 cut(s) 349, 877
MhlI GDGCHC 2 cut(s) 570, 664
MluCI AATT 7 cut(s) 28, 239, 575, 760, 976, 1190, 1246
MlyI GAGTC 4 cut(s) 487, 976, 1009, 1074
MmeI TCCRAC 1 cut(s) 90
MroI TCCGGA 1 cut(s) 1006
MseI TTAA 6 cut(s) 261, 323, 459, 489, 588, 942
MslI CAYNNNNRTG 1 cut(s) 1366
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 3 cut(s) 965, 1007, 1166
MspR9I CCNGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 28
MvaI CCWGG 1 cut(s) 197
NdeII GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
NlaIII CATG 3 cut(s) 341, 365, 1123
NlaIV GGNNCC 1 cut(s) 1347
NspI RCATGY 2 cut(s) 365, 1123
OliI CACNNNNGTG 1 cut(s) 1366
PaeR7I CTCGAG 1 cut(s) 569
PciI ACATGT 1 cut(s) 361
PfeI GAWTC 6 cut(s) 506, 611, 767, 1022, 1295, 1375
PflMI CCANNNNNTGG 2 cut(s) 1058, 1132
PinAI ACCGGT 1 cut(s) 964
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 4 cut(s) 487, 976, 1008, 1074
PpsI GAGTC 4 cut(s) 487, 976, 1008, 1074
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 1347
PspPI GGNCC 3 cut(s) 193, 437, 449
PspPPI RGGWCCY 1 cut(s) 437
PspXI VCTCGAGB 1 cut(s) 569
PsuI RGATCY 2 cut(s) 349, 877
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 1366
SaqAI TTAA 6 cut(s) 261, 323, 459, 489, 588, 942
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 6 cut(s) 199, 253, 349, 601, 877, 1239
Sau96I GGNCC 3 cut(s) 193, 437, 449
SchI GAGTC 4 cut(s) 487, 976, 1009, 1074
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 2 cut(s) 570, 664
SfaNI GCATC 2 cut(s) 177, 181
SfcI CTRYAG 2 cut(s) 1350, 1451
Sfr274I CTCGAG 1 cut(s) 569
SinI GGWCC 3 cut(s) 193, 437, 449
SlaI CTCGAG 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 1366
SmlI CTYRAG 7 cut(s) 331, 488, 569, 638, 932, 1040, 1364
SmoI CTYRAG 7 cut(s) 331, 488, 569, 638, 932, 1040, 1364
Sse9I AATT 7 cut(s) 28, 239, 575, 760, 976, 1190, 1246
SsiI CCGC 1 cut(s) 118
SspI AATATT 2 cut(s) 725, 736
SspMI CTAG 3 cut(s) 453, 783, 1013
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 3 cut(s) 314, 1075, 1126
TaaI ACNGT 2 cut(s) 668, 1354
TaqI TCGA 3 cut(s) 570, 1305, 1430
TasI AATT 7 cut(s) 28, 239, 575, 760, 976, 1190, 1246
TfiI GAWTC 6 cut(s) 506, 611, 767, 1022, 1295, 1375
Tru1I TTAA 6 cut(s) 261, 323, 459, 489, 588, 942
Tru9I TTAA 6 cut(s) 261, 323, 459, 489, 588, 942
TscAI CASTG 1 cut(s) 423
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 890
TspRI CASTG 1 cut(s) 423
Van91I CCANNNNNTGG 2 cut(s) 1058, 1132
Vha464I CTTAAG 1 cut(s) 488
VneI GTGCAC 1 cut(s) 660
VpaK11BI GGWCC 3 cut(s) 193, 437, 449
XapI RAATTY 3 cut(s) 760, 1190, 1246
XceI RCATGY 2 cut(s) 365, 1123
XhoI CTCGAG 1 cut(s) 569
XmiI GTMKAC 2 cut(s) 478, 714
XspI CTAG 3 cut(s) 453, 783, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.