AT3G54160

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000813
Physical Location & Seq
Forward (+)
20053020 .. 20055309
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54160.1

Sequence Viewer

Length: 1305 bp
ATGAGTGAGATCGTGAGAGTTCAAGAGATCGTTTCTAAACTCTGGAACCTAAACATAGCCTCCAAAAAATCCCCGAATCTGGTTACGATCATATCGAATGTTGGTTATAATCCTTCGTTTGAAACTAATGGAAGCTTCAAAGATTTCGTTTCTCGATTACTAGCTCGACGTGACAGTTACCGTATAAGGAGATTCTCTTTGAAACTACGTTCTTTGGAATTTGATTCAGCTAAGTACCATCTTGTCAACGAATGTCTACGCAATGTGTTGAATCGAGGTGTTTTGGATCTTGAATTGGACATCAATGTCAATGAAGTTTACAAATTGCCATTTGAGGTCTTCACTTGCAAGAAAGTTGTGAAATTGAAACTAGGTTCTGATTTTGTTATTGACAATATCCCTAAGAACGCTTTGCTTCCAGCTCTTAAGACTCTCTTTCTTGATAAAGTTCGGTTCATTGATAAAAGTGGTGGTTGTGCGTTTACAAGGCTTGTTTCTGCTTGCCCTGTGCTAGAGGATTTAGTGATATATAGGAACAACCGTGAAGATTGGGAATGGTCTCGCATTGTGTCTAGTAAGATCCTTAAGAAACTTACTATTCGGCGTGAAGACTGGGAAGATCTTGATGGCTCTAGTTATGAGTCAATTAGTTTTGATACTCCGAGTCTTGAATACTTCGAATACTTTGATGTTCTCCGAGATGGGTATCCAGTTGTTGATCTCAAGTCCCTTGTTGAAGCTAAGCTAGAACTTCCTCTTTTTATGGTTGGCGATACCTACGATGTTAAGAATCTGATTAAGGGCTTAGAAAATGTTCAGATTCTGAGCTTAGGCGTTGTTGACACGATGCAGGTGTTTTGTTTCTTCGAAGAAGCAGTACCAGTGTTTGAAAACCTGTTTCATTTATCACTTAGCACTGAAGTAGATTTCTGTTGGAATGTTCTACCAATTCTGCTTGAGAAATCTCCAAATCTAAAGACTCTCACCATTCAGGCTTTGCATTATACTACTAATCACTATGGGGATGAGAACACTGTTTGCGATTGTTTGGAGGTGTATTCTTTCTTATCGTCCTGCCATATTGAGGTTCTAAAGATAACCCAGTTCAAGGGAGATATCGGAGAGATGGTGCAAATAAAGCATATCTTGGAGAAATTGCCGAGTCTTGAGCTGTTGGAAATTCATGGTCAGGTAAAAGGAGATGACAAAAAGCTGCAGATCATGTTGGATCTCCTAATGTTCGAAAGAGCTTCGTCTGAATGCAAAGTCGAGGTCAAGTTTCCAGTTTGTTTTGCCTCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

434

Amino Acids

49.88

Weight (kDa)

5.41

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 83 - 224 8.1e-11 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AarI CACCTGC 1 cut(s) 841
AasI GACNNNNNNGTC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 841
AccI GTMKAC 1 cut(s) 256
AclWI GGATC 3 cut(s) 294, 574, 1236
AcsI RAATTY 2 cut(s) 218, 1179
AcuI CTGAAG 1 cut(s) 939
AfaI GTAC 2 cut(s) 236, 879
AfiI CCNNNNNNNGG 3 cut(s) 79, 1084, 1108
AflII CTTAAG 2 cut(s) 425, 584
AjiI CACGTC 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 3 cut(s) 294, 574, 1236
AlwNI CAGNNNCTG 1 cut(s) 823
ApeKI GCWGC 1 cut(s) 1213
ApoI RAATTY 2 cut(s) 218, 1179
ArsI GACNNNNNNTTYG 2 cut(s) 1257, 1289
Asp700I GAANNNNTTC 1 cut(s) 813
AsuHPI GGTGA 1 cut(s) 976
AsuII TTCGAA 3 cut(s) 678, 867, 1242
BarI GAAGNNNNNNTAC 2 cut(s) 861, 893
BbsI GAAGAC 2 cut(s) 331, 615
BbvCI CCTCAGC 1 cut(s) 1297
BbvI GCAGC 1 cut(s) 1200
BccI CCATC 4 cut(s) 246, 620, 695, 1120
BciVI GTATCC 1 cut(s) 717
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 6 cut(s) 161, 371, 512, 573, 633, 746
BfmI CTRYAG 1 cut(s) 1214
BfrI CTTAAG 2 cut(s) 425, 584
BfuAI ACCTGC 1 cut(s) 841
BfuI GTATCC 1 cut(s) 717
BglII AGATCT 1 cut(s) 619
BisI GCNGC 1 cut(s) 1214
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 1215
BmgBI CACGTC 1 cut(s) 170
BmiI GGNNCC 1 cut(s) 47
BmrI ACTGGG 2 cut(s) 622, 1096
BmsI GCATC 1 cut(s) 837
BmuI ACTGGG 2 cut(s) 622, 1096
BpiI GAAGAC 2 cut(s) 331, 615
Bpu10I CCTNAGC 2 cut(s) 829, 1297
Bpu1102I GCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 3 cut(s) 678, 867, 1242
BpuEI CTTGAG 3 cut(s) 707, 977, 1187
BsaBI GATNNNNATC 1 cut(s) 705
BsaI GGTCTC 1 cut(s) 564
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 1113, 1143
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 1084, 1108
Bse1I ACTGG 5 cut(s) 617, 710, 881, 1102, 1283
Bse3DI GCAATG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 705
BseGI GGATG 1 cut(s) 1030
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 3 cut(s) 79, 1084, 1108
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 815
BseNI ACTGG 5 cut(s) 617, 710, 881, 1102, 1283
BseXI GCAGC 1 cut(s) 1200
BslFI GGGAC 1 cut(s) 712
BslI CCNNNNNNNGG 3 cut(s) 79, 1084, 1108
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 1 cut(s) 712
BsmI GAATGC 1 cut(s) 1265
Bso31I GGTCTC 1 cut(s) 564
Bsp119I TTCGAA 3 cut(s) 678, 867, 1242
Bsp143I GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
Bsp1720I GCTNAGC 1 cut(s) 741
BspCNI CTCAG 1 cut(s) 816
BspLI GGNNCC 1 cut(s) 47
BspMAI CTGCAG 1 cut(s) 1218
BspMI ACCTGC 1 cut(s) 841
BspPI GGATC 3 cut(s) 294, 574, 1236
BspT104I TTCGAA 3 cut(s) 678, 867, 1242
BspTI CTTAAG 2 cut(s) 425, 584
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 1 cut(s) 268
BsrI ACTGG 5 cut(s) 617, 710, 881, 1102, 1283
BssMI GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
Bst4CI ACNGT 4 cut(s) 176, 182, 542, 1036
BstAFI CTTAAG 2 cut(s) 425, 584
BstBI TTCGAA 3 cut(s) 678, 867, 1242
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 8 cut(s) 231, 402, 741, 805, 824, 829, 911, 1297
BstF5I GGATG 1 cut(s) 1030
BstKTI GATC 9 cut(s) 12, 30, 90, 289, 582, 622, 721, 1221, 1231
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
BstMWI GCNNNNNNNGC 1 cut(s) 1138
BstSFI CTRYAG 1 cut(s) 1214
BstV1I GCAGC 1 cut(s) 1200
BstV2I GAAGAC 2 cut(s) 331, 615
BstX2I RGATCY 4 cut(s) 286, 579, 619, 1228
BstYI RGATCY 4 cut(s) 286, 579, 619, 1228
BsuI GTATCC 1 cut(s) 717
BtrI CACGTC 1 cut(s) 170
BtsCI GGATG 1 cut(s) 1030
BtsIMutI CAGTG 3 cut(s) 888, 915, 1032
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 1 cut(s) 502
CaiI CAGNNNCTG 1 cut(s) 823
Csp6I GTAC 2 cut(s) 235, 878
CviAII CATG 3 cut(s) 1184, 1222, 1302
CviQI GTAC 2 cut(s) 235, 878
DdeI CTNAG 8 cut(s) 231, 402, 741, 805, 824, 829, 911, 1297
DpnI GATC 9 cut(s) 11, 29, 89, 288, 581, 621, 720, 1220, 1230
DpnII GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 1117
Eco57I CTGAAG 1 cut(s) 939
EcoRV GATATC 1 cut(s) 1117
FaeI CATG 3 cut(s) 1187, 1225, 1305
FalI AAGNNNNNCTT 4 cut(s) 419, 451, 1130, 1162
FaqI GGGAC 1 cut(s) 712
FatI CATG 3 cut(s) 1183, 1221, 1301
FblI GTMKAC 1 cut(s) 256
Fnu4HI GCNGC 1 cut(s) 1214
FokI GGATG 1 cut(s) 1037
Fsp4HI GCNGC 1 cut(s) 1214
FspBI CTAG 6 cut(s) 161, 371, 512, 573, 633, 746
GluI GCNGC 1 cut(s) 1214
Hin1II CATG 3 cut(s) 1187, 1225, 1305
HincII GTYRAC 2 cut(s) 247, 841
HindII GTYRAC 2 cut(s) 247, 841
HindIII AAGCTT 1 cut(s) 133
HphI GGTGA 1 cut(s) 976
Hpy166II GTNNAC 5 cut(s) 247, 257, 319, 483, 841
Hpy188I TCNGA 8 cut(s) 379, 663, 698, 795, 819, 825, 1121, 1258
Hpy188III TCNNGA 9 cut(s) 13, 23, 43, 153, 290, 440, 623, 668, 1166
Hpy8I GTNNAC 5 cut(s) 247, 257, 319, 483, 841
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 1 cut(s) 123
HpyCH4III ACNGT 4 cut(s) 176, 182, 542, 1036
HpyCH4IV ACGT 2 cut(s) 169, 208
HpyCH4V TGCA 6 cut(s) 348, 850, 1000, 1132, 1216, 1263
HpyF10VI GCNNNNNNNGC 1 cut(s) 1138
HpyF3I CTNAG 8 cut(s) 231, 402, 741, 805, 824, 829, 911, 1297
HpySE526I ACGT 2 cut(s) 169, 208
Hsp92II CATG 3 cut(s) 1187, 1225, 1305
Kzo9I GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
Lsp1109I GCAGC 1 cut(s) 1200
LweI GCATC 1 cut(s) 837
MaeI CTAG 6 cut(s) 161, 371, 512, 573, 633, 746
MaeII ACGT 2 cut(s) 169, 208
MaeIII GTNAC 3 cut(s) 82, 170, 176
MalI GATC 9 cut(s) 11, 29, 89, 288, 581, 621, 720, 1220, 1230
MboI GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
MboII GAAGA 6 cut(s) 331, 557, 620, 629, 856, 881
MflI RGATCY 4 cut(s) 286, 579, 619, 1228
MluCI AATT 8 cut(s) 218, 293, 323, 362, 645, 948, 1154, 1179
MlyI GAGTC 5 cut(s) 424, 650, 673, 973, 1171
MmeI TCCRAC 3 cut(s) 914, 1155, 1206
MnlI CCTC 8 cut(s) 70, 269, 328, 508, 765, 1045, 1078, 1264
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 4 cut(s) 426, 585, 786, 798
MspCI CTTAAG 2 cut(s) 425, 584
Mva1269I GAATGC 1 cut(s) 1265
MwoI GCNNNNNNNGC 1 cut(s) 1138
NdeII GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
NlaIII CATG 3 cut(s) 1187, 1225, 1305
NlaIV GGNNCC 1 cut(s) 47
NmeAIII GCCGAG 1 cut(s) 1185
NmuCI GTSAC 1 cut(s) 170
NspV TTCGAA 3 cut(s) 678, 867, 1242
PaqCI CACCTGC 1 cut(s) 841
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PctI GAATGC 1 cut(s) 1265
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 6 cut(s) 76, 192, 224, 271, 790, 820
PkrI GCNGC 1 cut(s) 1215
PleI GAGTC 5 cut(s) 424, 649, 672, 973, 1170
PpsI GAGTC 5 cut(s) 424, 649, 672, 973, 1170
PsiI TTATAA 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 47
PstI CTGCAG 1 cut(s) 1218
PstNI CAGNNNCTG 1 cut(s) 823
PsuI RGATCY 4 cut(s) 286, 579, 619, 1228
RsaI GTAC 2 cut(s) 236, 879
RsaNI GTAC 2 cut(s) 235, 878
SaqAI TTAA 4 cut(s) 426, 585, 786, 798
SatI GCNGC 1 cut(s) 1214
Sau3AI GATC 9 cut(s) 9, 27, 87, 286, 579, 619, 718, 1218, 1228
SchI GAGTC 5 cut(s) 424, 650, 673, 973, 1171
SfaNI GCATC 1 cut(s) 837
SfcI CTRYAG 1 cut(s) 1214
SfuI TTCGAA 3 cut(s) 678, 867, 1242
SmlI CTYRAG 5 cut(s) 425, 584, 722, 956, 1166
SmoI CTYRAG 5 cut(s) 425, 584, 722, 956, 1166
Sse9I AATT 8 cut(s) 218, 293, 323, 362, 645, 948, 1154, 1179
SspMI CTAG 6 cut(s) 161, 371, 512, 573, 633, 746
TaaI ACNGT 4 cut(s) 176, 182, 542, 1036
TaiI ACGT 2 cut(s) 172, 211
TaqI TCGA 8 cut(s) 95, 154, 166, 274, 678, 867, 1242, 1269
TasI AATT 8 cut(s) 218, 293, 323, 362, 645, 948, 1154, 1179
TfiI GAWTC 6 cut(s) 76, 192, 224, 271, 790, 820
Tru1I TTAA 4 cut(s) 426, 585, 786, 798
Tru9I TTAA 4 cut(s) 426, 585, 786, 798
TscAI CASTG 3 cut(s) 888, 922, 1039
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 1 cut(s) 1213
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 4 cut(s) 327, 445, 890, 1172
TspRI CASTG 3 cut(s) 888, 922, 1039
Vha464I CTTAAG 2 cut(s) 425, 584
XapI RAATTY 2 cut(s) 218, 1179
XmiI GTMKAC 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 6 cut(s) 161, 371, 512, 573, 633, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.