AT3G59250

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000813
Physical Location & Seq
Reverse (-)
21901570 .. 21903284
1715 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59250.1

Sequence Viewer

Length: 1278 bp
ATGGATATTGGTCCAAAAGATAAAATCAGCAATCTACCAGAAGCTCTGATTTGCCACATATTGTCATTCCTTCCCATAGAAGATTCTGCTTTGACCTCAGTTCTCTCAAAGAGATGGCGCTATCTCTTTGCTTTCAGACCAAACCTTGTTTTCGATGACTCGGTATGTCTACGTCCACCATGTGGACATGGTGTTATTCCAGACTATGTCACATCTTGGATACTCAATGTGTTAGAGCGTGGGGTATTGGATCTTGACTTGCAAGTTAATGTGAATGGGGTTCGTCTGCCTTCAAAGGTCTTTGTGAGCAAGTCACTGGTTAGGCTGAGAATAGAATCTGGAAATGTTCGTGGCATTGATGTGGACGATGTTTTTCTCCCAAAGCTAAAGTCTCTCTATCTTCATACAATTATGTTGGGAAAAGGTGAAGATTGCTTCGAAAAACTTACTTCTGGTTGTCATGTCCTTGAGGAATTAGTTCTGAATAATGTCTACTCCAATGTTTGGAACCGCTCTGTGTCTTCCAAAACCCTCAAGAGACTAACGCTATGGTGTACAGAGTCGTATGATAAAAATCCGCATAGTGTTTTGTTTGATACACCAAATCTTGTCTACTTGAAATACTCCGATTATGTTGCGGCAAAGTATCCAAAAGTGAATTTCAGTTCGCTTGTTGATGCCACTATTGGTCTTGCAATGACAAGTGATCAGGATGCAAATGCAAGCTATGATGCTTTGGTCGGCAATGCAACAGACTTTCTAATGGGAATATGCAATGTTCAGATCCTTTGCTTATCTGCCAACAGTCTTGCGGTTCTTACTTTCTGCTGTGAATCAATACCGGTATTCAACAACCTGATTCAGTTAACTATTAAGACTAACCAAAGCGTAGGATGGGAATCATTGCCAGCTCTACTCAAGAACTGTCCAATTCTAGAAACCCTTGTCTTCGAAGGACTCCTGCACATGTATACAATAAAATGCGGGGATGTTGACGGTTGCCTATGCAAATATTCAGGGGAGGTTCCCAGTTGCTTATCATCAAGTCCAGTGAAGGTTTTAAAGATATTGAGATTTGGTGAATATGGTATAGTCAATCAGATAGAGATGATCAAGATCTTCCTAGAGACAATGCCGCATCTTGAACAACTGACAGTATACTACAATACACCAATTGGTGGTGATCTGATTGAAGAAGTGTCAAGCCAACTTAAGAACTTTGCTAGAGAAGCTTCACCAAATTGCGAGATCCAAGTAATCCCTGATGTCCTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

425

Amino Acids

47.32

Weight (kDa)

5.28

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 9 - 43 3.3e-07 F-box domain
LRR_At5g56370 PF24758 71 - 206 9.7e-11 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 182, 504, 1178
AccBSI CCGCTC 1 cut(s) 513
AccI GTMKAC 5 cut(s) 169, 492, 612, 971, 1158
AciI CCGC 6 cut(s) 511, 578, 638, 812, 984, 1136
AclWI GGATC 3 cut(s) 258, 778, 1243
AcsI RAATTY 1 cut(s) 658
AdeI CACNNNGTG 1 cut(s) 182
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 3 cut(s) 182, 504, 1178
AflII CTTAAG 1 cut(s) 1211
AflIII ACRYGT 1 cut(s) 966
AgeI ACCGGT 1 cut(s) 841
AgsI TTSAA 5 cut(s) 294, 619, 850, 1145, 1193
AluBI AGCT 6 cut(s) 44, 385, 726, 911, 1232, 1275
AluI AGCT 6 cut(s) 44, 385, 726, 911, 1232, 1275
Alw26I GTCTC 3 cut(s) 396, 532, 1121
AlwI GGATC 3 cut(s) 258, 778, 1243
ApoI RAATTY 1 cut(s) 658
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
AsiGI ACCGGT 1 cut(s) 841
Asp700I GAANNNNTTC 1 cut(s) 477
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 4 cut(s) 437, 1091, 1193, 1227
AsuII TTCGAA 2 cut(s) 438, 951
AvaII GGWCC 1 cut(s) 11
BbsI GAAGAC 2 cut(s) 513, 940
BccI CCATC 2 cut(s) 108, 888
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciVI GTATCC 2 cut(s) 213, 657
BclI TGATCA 2 cut(s) 706, 1110
BcoDI GTCTC 3 cut(s) 396, 532, 1121
BfaI CTAG 3 cut(s) 935, 1124, 1224
BfoI RGCGCY 1 cut(s) 121
BfrI CTTAAG 1 cut(s) 1211
BfuI GTATCC 2 cut(s) 213, 657
BglII AGATCT 1 cut(s) 1116
BisI GCNGC 2 cut(s) 639, 1136
BlsI GCNGC 2 cut(s) 640, 1137
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 509, 1026
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 4 cut(s) 667, 703, 721, 1147
BmuI ACTGGG 1 cut(s) 1023
BpiI GAAGAC 2 cut(s) 513, 940
Bpu10I CCTNAGC 1 cut(s) 1271
Bpu14I TTCGAA 2 cut(s) 438, 951
BpuEI CTTGAG 3 cut(s) 488, 518, 902
BsaBI GATNNNNATC 3 cut(s) 573, 898, 1115
BsaWI WCCGGW 1 cut(s) 841
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 504, 1178
Bse118I RCCGGY 1 cut(s) 841
Bse1I ACTGG 3 cut(s) 321, 1029, 1049
Bse3DI GCAATG 4 cut(s) 702, 751, 781, 902
Bse8I GATNNNNATC 3 cut(s) 573, 898, 1115
BseGI GGATG 3 cut(s) 718, 899, 994
BseJI GATNNNNATC 3 cut(s) 573, 898, 1115
BseLI CCNNNNNNNGG 3 cut(s) 182, 504, 1178
BseMI GCAATG 4 cut(s) 702, 751, 781, 902
BseMII CTCAG 2 cut(s) 111, 317
BseNI ACTGG 3 cut(s) 321, 1029, 1049
BsgI GTGCAG 1 cut(s) 947
BshTI ACCGGT 1 cut(s) 841
BsiSI CCGG 1 cut(s) 842
BslI CCNNNNNNNGG 3 cut(s) 182, 504, 1178
BsmAI GTCTC 3 cut(s) 396, 532, 1121
Bsp119I TTCGAA 2 cut(s) 438, 951
Bsp1407I TGTACA 1 cut(s) 554
Bsp143I GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
BspACI CCGC 6 cut(s) 511, 578, 638, 812, 984, 1136
BspCNI CTCAG 2 cut(s) 110, 318
BspLI GGNNCC 2 cut(s) 509, 1026
BspPI GGATC 3 cut(s) 258, 778, 1243
BspT104I TTCGAA 2 cut(s) 438, 951
BspTI CTTAAG 1 cut(s) 1211
BsrBI CCGCTC 1 cut(s) 513
BsrDI GCAATG 4 cut(s) 702, 751, 781, 902
BsrFI RCCGGY 1 cut(s) 841
BsrGI TGTACA 1 cut(s) 554
BsrI ACTGG 3 cut(s) 321, 1029, 1049
BssAI RCCGGY 1 cut(s) 841
BssMI GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
BssNAI GTATAC 2 cut(s) 972, 1159
Bst1107I GTATAC 2 cut(s) 972, 1159
Bst4CI ACNGT 4 cut(s) 806, 926, 998, 1156
BstAFI CTTAAG 1 cut(s) 1211
BstAUI TGTACA 1 cut(s) 554
BstBI TTCGAA 2 cut(s) 438, 951
BstC8I GCNNGC 2 cut(s) 724, 909
BstDEI CTNAG 3 cut(s) 97, 326, 1271
BstF5I GGATG 3 cut(s) 718, 899, 994
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 7 cut(s) 253, 709, 786, 1113, 1119, 1186, 1251
BstMAI GTCTC 3 cut(s) 396, 532, 1121
BstMBI GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
BstMWI GCNNNNNNNGC 1 cut(s) 1229
BstNSI RCATGY 1 cut(s) 970
BstV2I GAAGAC 2 cut(s) 513, 940
BstX2I RGATCY 4 cut(s) 250, 783, 1116, 1248
BstYI RGATCY 4 cut(s) 250, 783, 1116, 1248
BstZ17I GTATAC 2 cut(s) 972, 1159
BsuI GTATCC 2 cut(s) 213, 657
BtsCI GGATG 3 cut(s) 718, 899, 994
BtsIMutI CAGTG 2 cut(s) 314, 1056
Cac8I GCNNGC 2 cut(s) 724, 909
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 841
Cfr13I GGNCC 1 cut(s) 11
Csp6I GTAC 1 cut(s) 555
CspAI ACCGGT 1 cut(s) 841
CviAII CATG 4 cut(s) 180, 188, 461, 967
CviJI RGCY 8 cut(s) 44, 325, 385, 726, 911, 1206, 1232, 1275
CviKI_1 RGCY 8 cut(s) 44, 325, 385, 726, 911, 1206, 1232, 1275
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 3 cut(s) 97, 326, 1271
DpnI GATC 7 cut(s) 252, 708, 785, 1112, 1118, 1185, 1250
DpnII GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
DraI TTTAAA 1 cut(s) 1062
DraIII CACNNNGTG 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 11
FaeI CATG 4 cut(s) 183, 191, 464, 970
FatI CATG 4 cut(s) 179, 187, 460, 966
FauI CCCGC 1 cut(s) 977
FbaI TGATCA 2 cut(s) 706, 1110
FblI GTMKAC 5 cut(s) 169, 492, 612, 971, 1158
Fnu4HI GCNGC 2 cut(s) 639, 1136
FokI GGATG 3 cut(s) 725, 906, 1001
Fsp4HI GCNGC 2 cut(s) 639, 1136
FspBI CTAG 3 cut(s) 935, 1124, 1224
GlaI GCGC 1 cut(s) 119
GluI GCNGC 2 cut(s) 639, 1136
HaeII RGCGCY 1 cut(s) 121
HapII CCGG 1 cut(s) 842
HhaI GCGC 1 cut(s) 120
Hin1II CATG 4 cut(s) 183, 191, 464, 970
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 2 cut(s) 867, 994
HindII GTYRAC 2 cut(s) 867, 994
HindIII AAGCTT 1 cut(s) 1230
HinfI GANTC 8 cut(s) 83, 158, 335, 560, 833, 859, 899, 957
HpaI GTTAAC 1 cut(s) 867
HpaII CCGG 1 cut(s) 842
HphI GGTGA 4 cut(s) 437, 1091, 1193, 1227
Hpy188I TCNGA 7 cut(s) 48, 137, 483, 628, 783, 1101, 1188
Hpy188III TCNNGA 9 cut(s) 200, 254, 339, 535, 710, 919, 935, 1114, 1142
HpyAV CCTTC 4 cut(s) 80, 300, 947, 1048
HpyCH4III ACNGT 4 cut(s) 806, 926, 998, 1156
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 8 cut(s) 262, 695, 716, 722, 749, 774, 964, 1008
HpyF10VI GCNNNNNNNGC 1 cut(s) 1229
HpyF3I CTNAG 3 cut(s) 97, 326, 1271
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 4 cut(s) 183, 191, 464, 970
HspAI GCGC 1 cut(s) 118
Ksp22I TGATCA 2 cut(s) 706, 1110
KspAI GTTAAC 1 cut(s) 867
Kzo9I GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
LweI GCATC 4 cut(s) 667, 703, 721, 1147
MaeI CTAG 3 cut(s) 935, 1124, 1224
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 2 cut(s) 208, 312
MalI GATC 7 cut(s) 252, 708, 785, 1112, 1118, 1185, 1250
MbiI CCGCTC 1 cut(s) 513
MboI GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
MboII GAAGA 7 cut(s) 92, 392, 440, 513, 940, 1111, 1205
MfeI CAATTG 1 cut(s) 1173
MflI RGATCY 4 cut(s) 250, 783, 1116, 1248
MluCI AATT 6 cut(s) 408, 473, 658, 930, 1173, 1240
MlyI GAGTC 3 cut(s) 152, 569, 951
MnlI CCTC 4 cut(s) 106, 463, 542, 1015
MroXI GAANNNNTTC 1 cut(s) 477
MseI TTAA 5 cut(s) 267, 866, 873, 1061, 1212
MslI CAYNNNNRTG 1 cut(s) 359
MspCI CTTAAG 1 cut(s) 1211
MspI CCGG 1 cut(s) 842
MunI CAATTG 1 cut(s) 1173
MwoI GCNNNNNNNGC 1 cut(s) 1229
NdeII GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
NlaIII CATG 4 cut(s) 183, 191, 464, 970
NlaIV GGNNCC 2 cut(s) 509, 1026
NmuCI GTSAC 2 cut(s) 208, 312
NspI RCATGY 1 cut(s) 970
NspV TTCGAA 2 cut(s) 438, 951
PciI ACATGT 1 cut(s) 966
PdmI GAANNNNTTC 1 cut(s) 477
PfeI GAWTC 5 cut(s) 83, 335, 833, 859, 899
PflFI GACNNNGTC 1 cut(s) 206
PflMI CCANNNNNTGG 3 cut(s) 182, 504, 1178
PinAI ACCGGT 1 cut(s) 841
PkrI GCNGC 2 cut(s) 640, 1137
PleI GAGTC 3 cut(s) 152, 568, 951
PpsI GAGTC 3 cut(s) 152, 568, 951
PscI ACATGT 1 cut(s) 966
PspN4I GGNNCC 2 cut(s) 509, 1026
PspPI GGNCC 1 cut(s) 11
PsuI RGATCY 4 cut(s) 250, 783, 1116, 1248
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 5 cut(s) 267, 866, 873, 1061, 1212
SatI GCNGC 2 cut(s) 639, 1136
Sau3AI GATC 7 cut(s) 250, 706, 783, 1110, 1116, 1183, 1248
Sau96I GGNCC 1 cut(s) 11
SchI GAGTC 3 cut(s) 152, 569, 951
SfaNI GCATC 4 cut(s) 667, 703, 721, 1147
SfuI TTCGAA 2 cut(s) 438, 951
SinI GGWCC 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 4 cut(s) 467, 533, 917, 1211
SmoI CTYRAG 4 cut(s) 467, 533, 917, 1211
Sse9I AATT 6 cut(s) 408, 473, 658, 930, 1173, 1240
SsiI CCGC 6 cut(s) 511, 578, 638, 812, 984, 1136
SspI AATATT 1 cut(s) 1013
SspMI CTAG 3 cut(s) 935, 1124, 1224
TaaI ACNGT 4 cut(s) 806, 926, 998, 1156
TaiI ACGT 1 cut(s) 175
TaqI TCGA 3 cut(s) 153, 438, 951
TasI AATT 6 cut(s) 408, 473, 658, 930, 1173, 1240
TatI WGTACW 1 cut(s) 554
TauI GCSGC 2 cut(s) 641, 1138
TfiI GAWTC 5 cut(s) 83, 335, 833, 859, 899
Tru1I TTAA 5 cut(s) 267, 866, 873, 1061, 1212
Tru9I TTAA 5 cut(s) 267, 866, 873, 1061, 1212
TscAI CASTG 2 cut(s) 321, 1056
TseFI GTSAC 2 cut(s) 208, 312
Tsp45I GTSAC 2 cut(s) 208, 312
TspDTI ATGAA 1 cut(s) 392
TspRI CASTG 2 cut(s) 321, 1056
Tth111I GACNNNGTC 1 cut(s) 206
Van91I CCANNNNNTGG 3 cut(s) 182, 504, 1178
Vha464I CTTAAG 1 cut(s) 1211
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 658
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 1 cut(s) 970
XmiI GTMKAC 5 cut(s) 169, 492, 612, 971, 1158
XmnI GAANNNNTTC 1 cut(s) 477
XspI CTAG 3 cut(s) 935, 1124, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.