AT4G22280

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000813
Physical Location & Seq
Reverse (-)
11775969 .. 11778151
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22280.1

Sequence Viewer

Length: 1248 bp
ATGGATAGGATCAACGGTCTATCAGATGATATTATTTGCCACATTCTATCTCTCCTTTCGTTCAAAGAGGCCACCTCTACATCTGTCCTCTCAATGCGATGGCGTTATTTGTTTGCCTTCAGACCAAATCTCTGTTTAGATGATCAAGAAGTGGGTGGAGGAGACAGTTTCATCGATTTTGTGGACAGAGTATTGGTTGTGACAGGTAATTTCCCCATAAGGAGAATCTCAATAAAATGTCGTATGAGTATCGACACCGGTCATGTCACCCGCTGGATGGTTGACGTGCTGGAACATGGTGTCTCATATCTTGATATTGACATAATTTCCGAAGACGACATCGGTTTCGTGCCTCTTGAGATCTTCACTTGCAAGACATTGGTTGAGCTGAAACTCGCAAGAGGATTTTATGCCATGGTTCCTGATTATGTTTCTCTTCCATCGCTTAAGACTCTCTTTCTTAGTTCCATTTGGTTCTGTAACAGGGATTGTTGCCCTTTGGGAAGGCTTCTTTCTGCTTGCCCTGTGCTTGAGGAATTAACCATTATTGGTGGGTGCTGGCAACATATAGAATTTTGTCGCACCGTCTCCTCTTCGACCCTTAAGAAACTTACTATTACGTCTTCTTTTCACTATGAATATTGGGGCATCACTATCGATACACCGAGTCTTGCCTACTTAGAATACTCTGATCTAGTTCCAAGACACTTTCCGTTTGTGAATCTTGAATCCCTTGTCGAAGCTAAGCTCGCTCTTGATCTATGTTCTGATAGCAATCCAACAAATCTGATTAAGGGGTTAAGAAATGTTGAGGTCTTGGAGTTATCATCTGGTTATACTAGTCAGATATTATATGATTTCCGTGAAGCAATCCCAGTGTTGAGCAAGTTGTTTCGTTTATCTATTAAAAGTAATTCAAGACACTACTACATTTGGAATCTTCTGCCAATACTTCTAGAAAAGTCTCCAAAACTAGAGACTCTCGTCATCAAGGGTCCATTGTCCGCTGATAGATATGAACGAGAATATGGATTGTCATGTCCTGTGAAAGTATTGGAGATAACTGAGTATGGAGGAAAATATGAAGAGCTAGAGCAAATGGAACATTTCTTAAAGAAACTGCCATGTCTTGAGCTTGTCAAAGTTCGTGCTTCTGCAATAAACGACAAGGAAAAGTCTCGTATCACCAAAGATCTGCTAATGGTTCCTAGATCGTCCAATTGCAATATCAAGCTCAAGTTTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

415

Amino Acids

47.41

Weight (kDa)

5.98

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 90 - 227 2.3e-10 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 148 - 175 1e-05 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 271, 1005
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 103
AfiI CCNNNNNNNGG 1 cut(s) 277
AflII CTTAAG 2 cut(s) 446, 602
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 3 cut(s) 64, 728, 918
AhlI ACTAGT 1 cut(s) 839
AjiI CACGTC 1 cut(s) 286
AluBI AGCT 6 cut(s) 388, 743, 748, 1090, 1135, 1234
AluI AGCT 6 cut(s) 388, 743, 748, 1090, 1135, 1234
Alw26I GTCTC 6 cut(s) 156, 307, 592, 969, 971, 1182
AlwI GGATC 1 cut(s) 17
AoxI GGCC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AsiGI ACCGGT 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 995
AsuHPI GGTGA 2 cut(s) 259, 1177
AvaII GGWCC 1 cut(s) 995
BbsI GAAGAC 2 cut(s) 339, 615
BccI CCATC 3 cut(s) 93, 271, 448
BcgI CGANNNNNNTGC 2 cut(s) 637, 671
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 6 cut(s) 156, 307, 592, 969, 971, 1182
BcuI ACTAGT 1 cut(s) 839
BfaI CTAG 6 cut(s) 695, 840, 956, 974, 1091, 1209
BfrI CTTAAG 2 cut(s) 446, 602
BglII AGATCT 2 cut(s) 360, 1192
BlpI GCTNAGC 1 cut(s) 744
Bme18I GGWCC 1 cut(s) 995
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 995
BmiI GGNNCC 3 cut(s) 420, 996, 1206
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 1 cut(s) 657
BmuI ACTGGG 1 cut(s) 869
BoxI GACNNNNGTC 2 cut(s) 258, 983
BpiI GAAGAC 2 cut(s) 339, 615
BplI GAGNNNNNCTC 2 cut(s) 59, 91
Bpu1102I GCTNAGC 1 cut(s) 744
BpuEI CTTGAG 4 cut(s) 377, 551, 1151, 1220
Bsa29I ATCGAT 2 cut(s) 174, 657
BsaBI GATNNNNATC 1 cut(s) 774
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 257
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 875
Bse8I GATNNNNATC 1 cut(s) 774
BseCI ATCGAT 2 cut(s) 174, 657
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 1056
BseNI ACTGG 1 cut(s) 875
BseRI GAGGAG 2 cut(s) 174, 580
BshFI GGCC 1 cut(s) 71
BshTI ACCGGT 1 cut(s) 257
BshVI ATCGAT 2 cut(s) 174, 657
BsiSI CCGG 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 6 cut(s) 156, 307, 592, 969, 971, 1182
BsmBI CGTCTC 1 cut(s) 592
BsnI GGCC 1 cut(s) 71
Bsp143I GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
Bsp1720I GCTNAGC 1 cut(s) 744
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 2 cut(s) 271, 1005
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 1057
BspDI ATCGAT 2 cut(s) 174, 657
BspLI GGNNCC 3 cut(s) 420, 996, 1206
BspPI GGATC 1 cut(s) 17
BspQI GCTCTTC 1 cut(s) 1080
BspTI CTTAAG 2 cut(s) 446, 602
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 1 cut(s) 875
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
BssT1I CCWWGG 1 cut(s) 414
Bst4CI ACNGT 3 cut(s) 17, 167, 586
Bst6I CTCTTC 3 cut(s) 441, 598, 1080
BstAFI CTTAAG 2 cut(s) 446, 602
BstC8I GCNNGC 3 cut(s) 520, 560, 750
BstDEI CTNAG 4 cut(s) 461, 679, 744, 1065
BstDSI CCRYGG 1 cut(s) 414
BstF5I GGATG 1 cut(s) 282
BstKTI GATC 7 cut(s) 12, 145, 363, 694, 760, 1195, 1214
BstMAI GTCTC 6 cut(s) 156, 307, 592, 969, 971, 1182
BstMBI GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
BstMWI GCNNNNNNNGC 1 cut(s) 749
BstPAI GACNNNNGTC 2 cut(s) 258, 983
BstV2I GAAGAC 2 cut(s) 339, 615
BstX2I RGATCY 2 cut(s) 360, 1192
BstYI RGATCY 2 cut(s) 360, 1192
Bsu15I ATCGAT 2 cut(s) 174, 657
BsuRI GGCC 1 cut(s) 71
BsuTUI ATCGAT 2 cut(s) 174, 657
BtgI CCRYGG 1 cut(s) 414
BtgZI GCGATG 2 cut(s) 112, 426
BtrI CACGTC 1 cut(s) 286
BtsCI GGATG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 882
Cac8I GCNNGC 3 cut(s) 520, 560, 750
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 995
ClaI ATCGAT 2 cut(s) 174, 657
CspAI ACCGGT 1 cut(s) 257
CviAII CATG 5 cut(s) 263, 296, 415, 1038, 1125
CviJI RGCY 8 cut(s) 71, 388, 508, 743, 748, 1090, 1135, 1234
CviKI_1 RGCY 8 cut(s) 71, 388, 508, 743, 748, 1090, 1135, 1234
DdeI CTNAG 4 cut(s) 461, 679, 744, 1065
DpnI GATC 7 cut(s) 11, 144, 362, 693, 759, 1194, 1213
DpnII GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
Eam1104I CTCTTC 3 cut(s) 441, 598, 1080
EarI CTCTTC 3 cut(s) 441, 598, 1080
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 995
Eco57I CTGAAG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 5 cut(s) 266, 299, 418, 1041, 1128
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FatI CATG 5 cut(s) 262, 295, 414, 1037, 1124
FauI CCCGC 1 cut(s) 278
FbaI TGATCA 1 cut(s) 142
FokI GGATG 1 cut(s) 289
FspBI CTAG 6 cut(s) 695, 840, 956, 974, 1091, 1209
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 258
Hin1II CATG 5 cut(s) 266, 299, 418, 1041, 1128
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 7 cut(s) 225, 451, 667, 721, 728, 937, 979
HpaII CCGG 1 cut(s) 258
HphI GGTGA 2 cut(s) 259, 1177
Hpy166II GTNNAC 2 cut(s) 184, 283
Hpy188I TCNGA 7 cut(s) 25, 122, 331, 691, 769, 789, 846
Hpy188III TCNNGA 9 cut(s) 146, 311, 356, 422, 725, 755, 918, 956, 1130
Hpy8I GTNNAC 2 cut(s) 184, 283
HpyAV CCTTC 2 cut(s) 127, 498
HpyCH4III ACNGT 3 cut(s) 17, 167, 586
HpyCH4IV ACGT 2 cut(s) 285, 620
HpyCH4V TGCA 3 cut(s) 372, 1157, 1224
HpyF10VI GCNNNNNNNGC 1 cut(s) 749
HpyF3I CTNAG 4 cut(s) 461, 679, 744, 1065
HpySE526I ACGT 2 cut(s) 285, 620
Hsp92II CATG 5 cut(s) 266, 299, 418, 1041, 1128
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
LguI GCTCTTC 1 cut(s) 1080
LweI GCATC 1 cut(s) 657
MaeI CTAG 6 cut(s) 695, 840, 956, 974, 1091, 1209
MaeII ACGT 2 cut(s) 285, 620
MaeIII GTNAC 3 cut(s) 199, 265, 479
MalI GATC 7 cut(s) 11, 144, 362, 693, 759, 1194, 1213
MboI GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
MboII GAAGA 7 cut(s) 344, 355, 428, 585, 615, 932, 1097
MfeI CAATTG 1 cut(s) 1219
MflI RGATCY 2 cut(s) 360, 1192
MluCI AATT 6 cut(s) 208, 324, 536, 572, 913, 1219
MlyI GAGTC 3 cut(s) 445, 676, 973
MmeI TCCRAC 1 cut(s) 803
MseI TTAA 7 cut(s) 447, 539, 603, 792, 800, 906, 1112
MspA1I CMGCKG 2 cut(s) 273, 1007
MspCI CTTAAG 2 cut(s) 446, 602
MspI CCGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 1219
MwoI GCNNNNNNNGC 1 cut(s) 749
NcoI CCATGG 1 cut(s) 414
NdeII GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
NlaIII CATG 5 cut(s) 266, 299, 418, 1041, 1128
NlaIV GGNNCC 3 cut(s) 420, 996, 1206
NmuCI GTSAC 2 cut(s) 199, 265
PciSI GCTCTTC 1 cut(s) 1080
PfeI GAWTC 4 cut(s) 225, 721, 728, 937
PinAI ACCGGT 1 cut(s) 257
PleI GAGTC 3 cut(s) 445, 675, 973
PpsI GAGTC 3 cut(s) 445, 675, 973
PshAI GACNNNNGTC 2 cut(s) 258, 983
PspN4I GGNNCC 3 cut(s) 420, 996, 1206
PspPI GGNCC 1 cut(s) 995
PsuI RGATCY 2 cut(s) 360, 1192
SapI GCTCTTC 1 cut(s) 1080
SaqAI TTAA 7 cut(s) 447, 539, 603, 792, 800, 906, 1112
Sau3AI GATC 7 cut(s) 9, 142, 360, 691, 757, 1192, 1211
Sau96I GGNCC 1 cut(s) 995
SchI GAGTC 3 cut(s) 445, 676, 973
SfaNI GCATC 1 cut(s) 657
SinI GGWCC 1 cut(s) 995
SmlI CTYRAG 6 cut(s) 356, 446, 530, 602, 1130, 1235
SmoI CTYRAG 6 cut(s) 356, 446, 530, 602, 1130, 1235
SpeI ACTAGT 1 cut(s) 839
Sse9I AATT 6 cut(s) 208, 324, 536, 572, 913, 1219
SsiI CCGC 2 cut(s) 271, 1005
SspI AATATT 1 cut(s) 641
SspMI CTAG 6 cut(s) 695, 840, 956, 974, 1091, 1209
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 3 cut(s) 17, 167, 586
TaiI ACGT 2 cut(s) 288, 623
TaqI TCGA 5 cut(s) 174, 252, 596, 657, 738
TasI AATT 6 cut(s) 208, 324, 536, 572, 913, 1219
TfiI GAWTC 4 cut(s) 225, 721, 728, 937
Tru1I TTAA 7 cut(s) 447, 539, 603, 792, 800, 906, 1112
Tru9I TTAA 7 cut(s) 447, 539, 603, 792, 800, 906, 1112
TscAI CASTG 1 cut(s) 882
TseFI GTSAC 2 cut(s) 199, 265
Tsp45I GTSAC 2 cut(s) 199, 265
TspDTI ATGAA 4 cut(s) 160, 651, 1032, 1098
TspGWI ACGGA 2 cut(s) 702, 851
TspRI CASTG 1 cut(s) 882
Vha464I CTTAAG 2 cut(s) 446, 602
VpaK11BI GGWCC 1 cut(s) 995
XapI RAATTY 1 cut(s) 572
XbaI TCTAGA 1 cut(s) 955
XspI CTAG 6 cut(s) 695, 840, 956, 974, 1091, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.