AT5G25860

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000813
Physical Location & Seq
Reverse (-)
9016908 .. 9018424
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25860.1

Sequence Viewer

Length: 1347 bp
ATGGATACCAGAAAGGTGAAATCTAGCTCTAGAGATGCAGTAAACTGTCTTCCAGATGAGATACTTGCTAAGATCTTGTCCTATCTTCCCACAAAACGAGCTGTTTCTACATCTCTTATCTCCAAAAGGTGGAGGAATCTGTTTGCGTTGATGATTCAACTCTTTGAATCTCAACATCATCTCTATTTAGACGACTCAGATTTAGTTTATCCAGAGGAAGGTAAAGGAGAAATGAAAGATGTTCAGGAGAGCTTCGGGGATTTTGTTGATAAAACCCTTACCGATTGCAATACCATAAAGAAACTTTCAATATTATGTCCTCTTAAATGTTGCGCTCATAAGGACATCGACCAATGGCTACACCATGCCATGGAACGAGGTGTTGTGGATCTTGATATGCGTTTTAAAATGGGTTTTACTCCTACGCAAAATGAGTGGCCTTGTAATGTTTTCACAATCAAGACGTTGGTCAAGCTAACATTTAGAATAGAGGTTGGTCAAAATAAAATGCCATATTGTCCAAAAGGGGTTCTTCCAGTGCTAAAGAGTCTTTTTCTCCATGCAATTTGGTTTGCATGCGAAAGATTGTGCCATTCGATGCTTCCTGGTTGTCCAATACTTGAGGAATTATTCTTACATGACTTACGCGGTTACAGATATAATGACAGTCCAAACTTCTCTATATCCCACAAAACCCTCAAGAGACTCACAGTTCACTTCAATAACAGTTTTGAATTGGGGCGCCCAATGCAATTCGACACACCAAGTCTTCTTTACCTCGAATACTCTGGCTTCGCTCCTTGTCGCTATTCAAGGACCAGTTACTTGAATTCCCTTGTTGAGGTCAAACTAGATGTTCATATAAGACATGAATGTAATACTCACATGCATCTATCTCCGATCATTGATTGGATAGGCAACGTTAAGACCCTTTCCTTGTCTCCTGCTTCTGTTAAGATGATGTATTCAAGATGTGTGGAATTACATGTCTTTTCAAACCTTGTTAAGCTTTATTTTGAAAGTAATGAGAAAGAAGGTTGGGAAGTGCTTCCGCGTCTGCTTAACAAATCTCCAAAGCTAGAAACGTTGGTCCTCAAGGGTTTGCATTGTGCAAGTGATCACGGAGTTTGCGTTGATCGAAATGAAGTGAAGGTGTTGGAGATATATGGGTTTAGTGGATGTGGTAGAGAAGTGAGACAAGTGAAGTGCTTGTTGCGGGAAATGCAGTTTCTTCAAGTGATGAAAGTGGAAATTGATGCTCATGATGATAACAAGAAACTGAGAGTCATCAATCACCTTCTTGATCTTCCTAAACGTCCATCTAAATTTCATATCCAGTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

448

Amino Acids

51.94

Weight (kDa)

8.55

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 15 - 47 1.3e-07 F-box-like
F-box PF00646 16 - 48 7.3e-09 F-box domain
LRR_At5g56370 PF24758 116 - 242 1.1e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 741
AccB7I CCANNNNNTGG 2 cut(s) 129, 370
AccII CGCG 2 cut(s) 648, 1054
AciI CCGC 3 cut(s) 648, 1052, 1216
AclI AACGTT 2 cut(s) 921, 1085
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 2 cut(s) 829, 1325
AcyI GRCGYC 1 cut(s) 742
AfiI CCNNNNNNNGG 4 cut(s) 129, 218, 370, 841
AflIII ACRYGT 1 cut(s) 985
AjnI CCWGG 1 cut(s) 604
AluBI AGCT 6 cut(s) 27, 101, 252, 475, 1009, 1078
AluI AGCT 6 cut(s) 27, 101, 252, 475, 1009, 1078
Alw26I GTCTC 3 cut(s) 697, 945, 1189
AlwI GGATC 1 cut(s) 396
AoxI GGCC 1 cut(s) 437
ApoI RAATTY 2 cut(s) 829, 1325
Asp700I GAANNNNTTC 1 cut(s) 1047
AspLEI GCGC 2 cut(s) 335, 744
AspS9I GGNCC 2 cut(s) 816, 1090
AsuHPI GGTGA 2 cut(s) 28, 1286
AvaII GGWCC 2 cut(s) 816, 1090
BanI GGYRCC 1 cut(s) 741
BarI GAAGNNNNNNTAC 2 cut(s) 33, 65
BbsI GAAGAC 2 cut(s) 41, 761
BccI CCATC 1 cut(s) 1327
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 1 cut(s) 1117
BcoDI GTCTC 3 cut(s) 697, 945, 1189
BfaI CTAG 4 cut(s) 24, 30, 851, 1079
BfoI RGCGCY 1 cut(s) 745
BglII AGATCT 1 cut(s) 72
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 2 cut(s) 816, 1090
BmgT120I GGNCC 2 cut(s) 816, 1090
BmiI GGNNCC 1 cut(s) 743
BmrFI CCNGG 1 cut(s) 606
BmsI GCATC 4 cut(s) 25, 588, 898, 1246
BoxI GACNNNNGTC 1 cut(s) 467
BpiI GAAGAC 2 cut(s) 41, 761
BpuEI CTTGAG 3 cut(s) 641, 683, 1079
BsaHI GRCGYC 1 cut(s) 742
BsaJI CCNNGG 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 4 cut(s) 129, 218, 370, 841
Bse1I ACTGG 3 cut(s) 536, 819, 1336
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 1184
BseLI CCNNNNNNNGG 4 cut(s) 129, 218, 370, 841
BseMII CTCAG 2 cut(s) 210, 1271
BseNI ACTGG 3 cut(s) 536, 819, 1336
Bsh1236I CGCG 2 cut(s) 648, 1054
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 741
BslI CCNNNNNNNGG 4 cut(s) 129, 218, 370, 841
BsmAI GTCTC 3 cut(s) 697, 945, 1189
BsnI GGCC 1 cut(s) 439
Bsp143I GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 3 cut(s) 648, 1052, 1216
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 2 cut(s) 209, 1272
BspFNI CGCG 2 cut(s) 648, 1054
BspHI TCATGA 1 cut(s) 1261
BspLI GGNNCC 1 cut(s) 743
BspPI GGATC 1 cut(s) 396
BspT107I GGYRCC 1 cut(s) 741
BsrI ACTGG 3 cut(s) 536, 819, 1336
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
BssNI GRCGYC 1 cut(s) 742
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 4 cut(s) 47, 668, 712, 728
BstACI GRCGYC 1 cut(s) 742
BstC8I GCNNGC 1 cut(s) 577
BstDEI CTNAG 3 cut(s) 69, 196, 1280
BstDSI CCRYGG 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 839
BstF5I GGATG 1 cut(s) 1184
BstFNI CGCG 2 cut(s) 648, 1054
BstH2I RGCGCY 1 cut(s) 745
BstHHI GCGC 2 cut(s) 335, 744
BstKTI GATC 6 cut(s) 75, 391, 903, 1120, 1138, 1306
BstMAI GTCTC 3 cut(s) 697, 945, 1189
BstMBI GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
BstMWI GCNNNNNNNGC 2 cut(s) 748, 1222
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 3 cut(s) 579, 889, 989
BstPAI GACNNNNGTC 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 604
BstUI CGCG 2 cut(s) 648, 1054
BstV2I GAAGAC 2 cut(s) 41, 761
BstX2I RGATCY 2 cut(s) 72, 388
BstYI RGATCY 2 cut(s) 72, 388
BsuRI GGCC 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 1184
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 1 cut(s) 577
CciI TCATGA 1 cut(s) 1261
CfoI GCGC 2 cut(s) 335, 744
Cfr13I GGNCC 2 cut(s) 816, 1090
CseI GACGC 1 cut(s) 1043
CspCI CAANNNNNGTGG 4 cut(s) 416, 451, 957, 992
CviAII CATG 9 cut(s) 365, 370, 560, 576, 638, 869, 886, 986, 1262
CviJI RGCY 9 cut(s) 27, 101, 252, 358, 439, 475, 792, 1009, 1078
CviKI_1 RGCY 9 cut(s) 27, 101, 252, 358, 439, 475, 792, 1009, 1078
DdeI CTNAG 3 cut(s) 69, 196, 1280
DinI GGCGCC 1 cut(s) 743
DpnI GATC 6 cut(s) 74, 390, 902, 1119, 1137, 1305
DpnII GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
DraI TTTAAA 1 cut(s) 406
Eco130I CCWWGG 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 816, 1090
EcoNI CCTNNNNNAGG 1 cut(s) 839
EcoRI GAATTC 1 cut(s) 829
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 891
EgeI GGCGCC 1 cut(s) 743
EheI GGCGCC 1 cut(s) 743
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 9 cut(s) 368, 373, 563, 579, 641, 872, 889, 989, 1265
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FatI CATG 9 cut(s) 364, 369, 559, 575, 637, 868, 885, 985, 1261
FauI CCCGC 1 cut(s) 1209
FbaI TGATCA 1 cut(s) 1117
FokI GGATG 1 cut(s) 1191
FspBI CTAG 4 cut(s) 24, 30, 851, 1079
GlaI GCGC 2 cut(s) 334, 743
HaeII RGCGCY 1 cut(s) 745
HaeIII GGCC 1 cut(s) 439
HgaI GACGC 1 cut(s) 1043
HhaI GCGC 2 cut(s) 335, 744
Hin1I GRCGYC 1 cut(s) 742
Hin1II CATG 9 cut(s) 368, 373, 563, 579, 641, 872, 889, 989, 1265
Hin6I GCGC 2 cut(s) 333, 742
HinP1I GCGC 2 cut(s) 333, 742
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 7 cut(s) 136, 154, 167, 194, 547, 705, 1284
HphI GGTGA 2 cut(s) 28, 1286
Hpy166II GTNNAC 2 cut(s) 43, 715
Hpy188I TCNGA 2 cut(s) 199, 900
Hpy8I GTNNAC 2 cut(s) 43, 715
HpyAV CCTTC 4 cut(s) 212, 1028, 1144, 1307
HpyCH4III ACNGT 4 cut(s) 47, 668, 712, 728
HpyCH4IV ACGT 4 cut(s) 464, 921, 1085, 1315
HpyCH4V TGCA 9 cut(s) 38, 288, 563, 575, 751, 889, 1105, 1112, 1225
HpyF10VI GCNNNNNNNGC 2 cut(s) 748, 1222
HpyF3I CTNAG 3 cut(s) 69, 196, 1280
HpySE526I ACGT 4 cut(s) 464, 921, 1085, 1315
Hsp92I GRCGYC 1 cut(s) 742
Hsp92II CATG 9 cut(s) 368, 373, 563, 579, 641, 872, 889, 989, 1265
HspAI GCGC 2 cut(s) 333, 742
KasI GGCGCC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 1117
Kzo9I GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
LmnI GCTCC 1 cut(s) 802
LweI GCATC 4 cut(s) 25, 588, 898, 1246
MaeI CTAG 4 cut(s) 24, 30, 851, 1079
MaeII ACGT 4 cut(s) 464, 921, 1085, 1315
MaeIII GTNAC 2 cut(s) 650, 821
MalI GATC 6 cut(s) 74, 390, 902, 1119, 1137, 1305
MboI GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
MboII GAAGA 6 cut(s) 41, 77, 524, 761, 1223, 1298
MflI RGATCY 2 cut(s) 72, 388
MluCI AATT 8 cut(s) 564, 626, 734, 752, 829, 980, 1251, 1325
Mly113I GGCGCC 1 cut(s) 742
MlyI GAGTC 4 cut(s) 188, 556, 699, 1293
MmeI TCCRAC 1 cut(s) 1137
Mph1103I ATGCAT 1 cut(s) 891
MroXI GAANNNNTTC 1 cut(s) 1047
MseI TTAA 6 cut(s) 324, 405, 924, 954, 1005, 1062
MspR9I CCNGG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 2 cut(s) 648, 1054
MwoI GCNNNNNNNGC 2 cut(s) 748, 1222
NarI GGCGCC 1 cut(s) 742
NcoI CCATGG 1 cut(s) 369
NdeII GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
NlaIII CATG 9 cut(s) 368, 373, 563, 579, 641, 872, 889, 989, 1265
NlaIV GGNNCC 1 cut(s) 743
NsiI ATGCAT 1 cut(s) 891
NspI RCATGY 3 cut(s) 579, 889, 989
PaeI GCATGC 1 cut(s) 579
PagI TCATGA 1 cut(s) 1261
PciI ACATGT 1 cut(s) 985
PcsI WCGNNNNNNNCGW 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 1047
PfeI GAWTC 3 cut(s) 136, 154, 167
PflMI CCANNNNNTGG 2 cut(s) 129, 370
PleI GAGTC 4 cut(s) 188, 555, 699, 1292
PluTI GGCGCC 1 cut(s) 745
PpsI GAGTC 4 cut(s) 188, 555, 699, 1292
PscI ACATGT 1 cut(s) 985
PshAI GACNNNNGTC 1 cut(s) 467
Psp1406I AACGTT 2 cut(s) 921, 1085
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 1 cut(s) 743
PspPI GGNCC 2 cut(s) 816, 1090
PsuI RGATCY 2 cut(s) 72, 388
SaqAI TTAA 6 cut(s) 324, 405, 924, 954, 1005, 1062
Sau3AI GATC 6 cut(s) 72, 388, 900, 1117, 1135, 1303
Sau96I GGNCC 2 cut(s) 816, 1090
SchI GAGTC 4 cut(s) 188, 556, 699, 1293
ScrFI CCNGG 1 cut(s) 606
SfaNI GCATC 4 cut(s) 25, 588, 898, 1246
SfoI GGCGCC 1 cut(s) 743
SinI GGWCC 2 cut(s) 816, 1090
SmlI CTYRAG 3 cut(s) 620, 698, 1094
SmoI CTYRAG 3 cut(s) 620, 698, 1094
SphI GCATGC 1 cut(s) 579
Sse9I AATT 8 cut(s) 564, 626, 734, 752, 829, 980, 1251, 1325
SsiI CCGC 3 cut(s) 648, 1052, 1216
SspDI GGCGCC 1 cut(s) 741
SspI AATATT 1 cut(s) 312
SspMI CTAG 4 cut(s) 24, 30, 851, 1079
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 47, 668, 712, 728
TaiI ACGT 4 cut(s) 467, 924, 1088, 1318
TaqI TCGA 5 cut(s) 348, 596, 756, 780, 1138
TasI AATT 8 cut(s) 564, 626, 734, 752, 829, 980, 1251, 1325
TfiI GAWTC 3 cut(s) 136, 154, 167
Tru1I TTAA 6 cut(s) 324, 405, 924, 954, 1005, 1062
Tru9I TTAA 6 cut(s) 324, 405, 924, 954, 1005, 1062
TscAI CASTG 1 cut(s) 543
TspDTI ATGAA 6 cut(s) 248, 848, 885, 1158, 1256, 1319
TspGWI ACGGA 1 cut(s) 1137
TspRI CASTG 1 cut(s) 543
Van91I CCANNNNNTGG 2 cut(s) 129, 370
VpaK11BI GGWCC 2 cut(s) 816, 1090
XagI CCTNNNNNAGG 1 cut(s) 839
XapI RAATTY 2 cut(s) 829, 1325
XbaI TCTAGA 1 cut(s) 29
XceI RCATGY 3 cut(s) 579, 889, 989
XmnI GAANNNNTTC 1 cut(s) 1047
XspI CTAG 4 cut(s) 24, 30, 851, 1079
Zsp2I ATGCAT 1 cut(s) 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.