Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001165

Physical Location & Seq

Genomic Coordinates Forward (+)

19723350 .. 19727128

Gene Structure 3779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G53230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2448 bp

ATGGCGAATCAAGCTGAATCATCTGATTCGAAGGGGACGAAGAAGGATTTTAGCACCGCGATTCTGGAGAAAAAGAAGGCAGCGAATCGTTTGGTTGTGGATGAAGCCATTAACGATGATAACTCCGTCGTTTCTCTTCACCCTGATACCATGGAGAAGCTACAGCTTTTTCGTGGTGATACTATCTTAATCAAGGGAAAGAAGAGGAAGGATACTGTGTGTATTGCTTTAGCTGATGAGACATGTGATGAACCAAAAATTAGAATGAACAAGGTTGTTAGGTCTAATCTCAGAGTTAGGCTTGGGGATGTTATTTCTGTTCATCAATGTCCTGATGTCAAGTATGGGAACCGTGTTCACATCTTACCTTTAGATGATACCATTGAAGGAGTAAGTGGCAATATATTTGATGCATACCTTAAACCTTATTTCTTAGAGGCATATCGTCCGGTGAGGAAGGGCGATCTTTTCCTAGTTAGAGGAGGGATGAGAAGTATTGAGTTTAAGGTTATTGAAACTGATCCTGCTGAGTACTGTGTGGTTGCTCCAGATACAGAAATTTTCTGTGAAGGTGAACCGATAAAGAGAGAGGATGAGGAGAGGTTGGATGAAGTTGGGTATGATGATGTTGGTGGTGTCAGGAAACAAATGGCTCAGATTCGTGAACTTGTTGAGCTCCCATTGAGGCATCCACAGCTCTTCAAGTCTATTGGTGTGAAACCTCCCAAAGGAATCTTGCTCTATGGCCCTCCTGGTTCTGGGAAGACACTTATTGCCCGTGCTGTTGCTAATGAGACTGGTGCATTTTTCTTCTGTATCAATGGTCCCGAGATCATGTCGAAGCTAGCTGGTGAGAGTGAAAGTAACCTCAGGAAAGCGTTTGAGGAAGCTGAGAAGAATGCCCCTTCGATCATATTCATTGATGAGATCGATTCGATTGCTCCAAAAAGAGAGAAGACTCACGGAGAAGTTGAAAGACGGATTGTATCCCAGCTCTTGACACTTATGGATGGTCTTAAATCCAGAGCCCATGTGATTGTTATGGGAGCTACCAACCGTCCCAACAGCATTGACCCTGCTTTGAGAAGATTTGGACGTTTTGATAGAGAAATTGATATTGGTGTTCCTGATGAGATTGGTCGTCTTGAAGTTCTCAGGATTCACACCAAGAACATGAAGCTTGCGGAAGATGTTGATTTGGAAAGAGTATCTAAAGACACCCATGGGTATGTTGGTGCTGATCTTGCAGCATTATGCACTGAAGCTGCTCTCCAATGCATTAGGGAGAAGATGGATGTAATTGACCTAGATGACGAGGAGATAGATGCTGAAATTCTCAATTCTATGGCTGTCAGTAATGATCATTTCCAGACTGCCCTTGGTAACAGCAATCCTTCAGCTCTACGTGAGACTGTCGTTGAGGTGCCTAATGTGTCTTGGGAGGACATTGGTGGTCTTGAGAATGTCAAGAGGGAGCTCCAAGAGACTGTTCAATATCCAGTGGAGCATCCTGAGAAATTCGAGAAGTTTGGGATGTCACCGTCTAAAGGAGTCCTATTCTATGGCCCCCCTGGTTGTGGAAAGACCCTCTTGGCTAAGGCTATTGCGAACGAGTGTCAAGCCAACTTCATTAGTATCAAGGGTCCTGAGCTCCTCACAATGTGGTTTGGAGAGAGTGAAGCCAATGTGAGAGAGATATTCGACAAGGCACGACAGTCTGCCCCTTGTGTTCTTTTCTTTGATGAGCTGGACTCCATCGCCACTCAGAGAGGGAACAGTGTTGGAGATGCTGGTGGTGCAGCAGATAGAGTATTGAACCAGCTTCTTACGGAGATGGATGGAATGAATGCTAAGAAGACGGTCTTCATCATCGGAGCTACAAACAGACCAGACATTATTGATCCTGCACTTCTGCGTCCTGGTCGTCTCGATCAGCTCATCTACATTCCTCTTCCTGATGAGGAGTCTCGTTACCAGATCTTTAAGTCTTGCTTGAGGAAATCACCTGTGGCGAAAGATGTTGACCTGAGGGCTCTTGCTAAGTACACCCAAGGCTTCAGTGGTGCGGACATCACAGAGATTTGCCAGCGTTCTTGCAAGTATGCGATCAGAGAAAACATCGAGAAGGATATTGAGAAGGAGAGGAAGAGAGCAGAGTCGCCAGAAGCAATGGAAGAGGATGAGGAGGAGATTGCAGAGATTAAAGCTGGTCACTTTGAGGAATCCATGAAATACGCTAGGAGATCAGTGAGTGATGCAGACATTCGCAAGTATCAGGCTTTTGCTCAGACTCTCCAGCAATCACGTGGGTTTGGAAGCGAGTTCAGGTTTCCAGATGCGCCGACAGGGACTACTGGAGCTTTCCCTGGAGCTGCTGCCACAGTTGGGGGAGTCGACCCGTTTGCCACATCCGGAGGAGCAGCGGATGATGATGATCTCTACAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010038 GO:0010604 GO:0012505 GO:0016020 GO:0019222 GO:0031974 GO:0031981 GO:0042176 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046686 GO:0048518 GO:0050789 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0070013 GO:0071944 GO:0080090

KEGG Pathways

Metabolic & Signaling

ko04141 ko05134 map04141 map05134

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

815

Amino Acids

90.34

Weight (kDa)

5.04

Isoelectric Point (pI)

43.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_N	PF02359	30 - 111	3.1e-23	Cell division protein 48 (CDC48), N-terminal domain
CDC48_2	PF02933	132 - 195	2.3e-11	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	245 - 374	1.4e-47	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	397 - 440	2e-15	AAA+ lid domain
AAA	PF00004	518 - 651	4.7e-47	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	673 - 713	8.8e-12	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53230
fragaria_vesca	FvH4_2g06940 FvH4_2g06950 FvH4_2g07260 FvH4_4g17680 FvH4_6g16220 FvH4_6g16220
malus_domestica	MD03G1125700.v1.1 MD04G1094400.v1.1 MD11G1142500.v1.1 MD12G1114300.v1.1 MD17G1225300.v1.1 MD17G1225400.v1.1 MD17G1225600.v1.1 MD17G1228800.v1.1
prunus_persica	Prupe.4G237600_v2.0.a1 Prupe.6G107800_v2.0.a1 Prupe.6G233700_v2.0.a1 Prupe.6G233700_v2.0.a1
pyrus_communis	pycom04g08940 pycom04g08950 pycom09g14580 pycom11g11820 pycom12g11360
rosa_chinensis	RchiOBHm_Chr3g0470061 RchiOBHm_Chr4g0421621 RchiOBHm_Chr6g0259281 RchiOBHm_Chr6g0259871
rosa_laevigata	RLG00000007663 RLG00000014503 RLG00000014543 RLG00000024300
rosa_multiflora	Rmu_co8522155.1_g000003 Rmu_sc0005600.1_g000001 Rmu_sc0009049.1_g000005 Rmu_sc0011974.1_g000005 Rmu_sc0033604.1_g000001 Rmu_sc0033605.1_g000001 Rmu_ssc0000050.1_g000089
rosa_roxburghii	Rroxscaffold_5G00363870 Rroxscaffold_6G00411220
rosa_rugosa	Rorug03G0106600 Rorug04G0173900 Rorug05G0586800 Rorug05G0590900
rosa_samantha	Rh3AG155800 Rh4CG248200 Rh6AG103300 Rh6AG103400 Rh6AG107600 Rh6BG095700 Rh6CG092600 Rh6CG096400
rosa_wichuraiana	Rw3G014670 Rw4G020040 Rw6G008920 Rw6G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1423
AccI	GTMKAC	1 cut(s)	2394
AccII	CGCG	1 cut(s)	59
AccIII	TCCGGA	1 cut(s)	2411
AciI	CCGC	4 cut(s)	57, 1184, 2066, 2423
AclWI	GGATC	2 cut(s)	515, 1895
AcsI	RAATTY	3 cut(s)	558, 1332, 1517
AcuI	CTGAAG	3 cut(s)	1281, 1380, 2041
AcvI	CACGTG	1 cut(s)	2306
AdeI	CACNNNGTG	1 cut(s)	1662
AfaI	GTAC	2 cut(s)	533, 2045
AfiI	CCNNNNNNNGG	5 cut(s)	728, 758, 1547, 1577, 2385
AflIII	ACRYGT	1 cut(s)	242
AgsI	TTSAA	7 cut(s)	386, 515, 703, 974, 1148, 1493, 1816
AjnI	CCWGG	4 cut(s)	751, 1570, 1918, 2365
AjuI	GAANNNNNNNTTGG	4 cut(s)	1101, 1133, 1473, 1505
Alw21I	GWGCWC	3 cut(s)	678, 1479, 1653
Alw26I	GTCTC	6 cut(s)	233, 788, 1403, 1478, 1931, 1971
AlwI	GGATC	2 cut(s)	515, 1895
Ama87I	CYCGRG	1 cut(s)	827
Aor13HI	TCCGGA	1 cut(s)	2411
AoxI	GGCC	2 cut(s)	745, 1564
ApeKI	GCWGC	7 cut(s)	80, 1247, 1265, 1799, 2372, 2375, 2420
ApoI	RAATTY	3 cut(s)	558, 1332, 1517
AspLEI	GCGC	1 cut(s)	2341
AspS9I	GGNCC	4 cut(s)	746, 824, 1565, 1643
AsuHPI	GGTGA	7 cut(s)	131, 188, 463, 584, 863, 1530, 1995
AsuII	TTCGAA	1 cut(s)	29
AsuNHI	GCTAGC	1 cut(s)	844
AvaI	CYCGRG	1 cut(s)	827
AvaII	GGWCC	2 cut(s)	824, 1643
AxyI	CCTNAGG	2 cut(s)	869, 2027
BanI	GGYRCC	1 cut(s)	1423
BanII	GRGCYC	5 cut(s)	678, 1030, 1479, 1653, 2035
BbrPI	CACGTG	1 cut(s)	2306
BbsI	GAAGAC	4 cut(s)	770, 962, 1855, 1862
Bbv12I	GWGCWC	3 cut(s)	678, 1479, 1653
BbvI	GCAGC	7 cut(s)	92, 1252, 1259, 1811, 2359, 2362, 2432
BccI	CCATC	5 cut(s)	1004, 1285, 1763, 1828, 1832
BcgI	CGANNNNNNTGC	4 cut(s)	920, 954, 2384, 2418
BciT130I	CCWGG	4 cut(s)	753, 1572, 1920, 2367
BciVI	GTATCC	2 cut(s)	205, 997
BclI	TGATCA	1 cut(s)	1360
BcoDI	GTCTC	6 cut(s)	233, 788, 1403, 1478, 1931, 1971
BfaI	CTAG	4 cut(s)	473, 845, 1307, 2238
BfmI	CTRYAG	2 cut(s)	161, 2440
BfuI	GTATCC	2 cut(s)	205, 997
BglII	AGATCT	1 cut(s)	1977
BisI	GCNGC	7 cut(s)	81, 1248, 1266, 1800, 2373, 2376, 2421
BlsI	GCNGC	7 cut(s)	82, 1249, 1267, 1801, 2374, 2377, 2422
BmcAI	AGTACT	1 cut(s)	533
Bme1390I	CCNGG	4 cut(s)	753, 1572, 1920, 2367
Bme18I	GGWCC	2 cut(s)	824, 1643
BmeT110I	CYCGRG	1 cut(s)	827
BmgT120I	GGNCC	4 cut(s)	746, 824, 1565, 1643
BmiI	GGNNCC	5 cut(s)	350, 826, 1425, 1567, 1644
BmrFI	CCNGG	4 cut(s)	753, 1572, 1920, 2367
BmsI	GCATC	7 cut(s)	400, 697, 1315, 1516, 1777, 2245, 2326
BmtI	GCTAGC	1 cut(s)	848
BpiI	GAAGAC	4 cut(s)	770, 962, 1855, 1862
BplI	GAGNNNNNCTC	2 cut(s)	1736, 1768
BpmI	CTGGAG	5 cut(s)	86, 531, 2279, 2376, 2388
Bpu10I	CCTNAGC	2 cut(s)	1596, 1647
Bpu14I	TTCGAA	1 cut(s)	29
BpuEI	CTTGAG	2 cut(s)	1478, 2014
Bsa29I	ATCGAT	1 cut(s)	930
BsaAI	YACGTR	2 cut(s)	1406, 2306
BsaBI	GATNNNNATC	1 cut(s)	2433
BsaJI	CCNNGG	6 cut(s)	150, 1222, 1378, 1570, 2050, 2365
BsaWI	WCCGGW	2 cut(s)	448, 2411
Bsc4I	CCNNNNNNNGG	5 cut(s)	728, 758, 1547, 1577, 2385
Bse1I	ACTGG	3 cut(s)	802, 1499, 2359
Bse21I	CCTNAGG	2 cut(s)	869, 2027
Bse3DI	GCAATG	1 cut(s)	2175
Bse8I	GATNNNNATC	1 cut(s)	2433
BseAI	TCCGGA	1 cut(s)	2411
BseBI	CCWGG	4 cut(s)	753, 1572, 1920, 2367
BseCI	ATCGAT	1 cut(s)	930
BseDI	CCNNGG	6 cut(s)	150, 1222, 1378, 1570, 2050, 2365
BseJI	GATNNNNATC	1 cut(s)	2433
BseLI	CCNNNNNNNGG	5 cut(s)	728, 758, 1547, 1577, 2385
BseMI	GCAATG	1 cut(s)	2175
BseNI	ACTGG	3 cut(s)	802, 1499, 2359
BseRI	GAGGAG	8 cut(s)	495, 611, 1331, 1643, 1976, 2198, 2201, 2430
BseXI	GCAGC	7 cut(s)	92, 1252, 1259, 1811, 2359, 2362, 2432
BseYI	CCCAGC	1 cut(s)	990
BsgI	GTGCAG	2 cut(s)	1818, 1890
Bsh1236I	CGCG	1 cut(s)	59
BshFI	GGCC	2 cut(s)	747, 1566
BshNI	GGYRCC	1 cut(s)	1423
BshVI	ATCGAT	1 cut(s)	930
BsiHKAI	GWGCWC	3 cut(s)	678, 1479, 1653
BsiHKCI	CYCGRG	1 cut(s)	827
BsiSI	CCGG	2 cut(s)	449, 2412
BslFI	GGGAC	4 cut(s)	49, 810, 1044, 2362
BslI	CCNNNNNNNGG	5 cut(s)	728, 758, 1547, 1577, 2385
BsmAI	GTCTC	6 cut(s)	233, 788, 1403, 1478, 1931, 1971
BsmBI	CGTCTC	1 cut(s)	1931
BsmFI	GGGAC	4 cut(s)	49, 810, 1044, 2362
BsmI	GAATGC	2 cut(s)	904, 1852
BsnI	GGCC	2 cut(s)	747, 1566
BsoBI	CYCGRG	1 cut(s)	827
Bsp119I	TTCGAA	1 cut(s)	29
Bsp1286I	GDGCHC	5 cut(s)	678, 1030, 1479, 1653, 2035
Bsp13I	TCCGGA	1 cut(s)	2411
Bsp19I	CCATGG	2 cut(s)	150, 1222
BspACI	CCGC	4 cut(s)	57, 1184, 2066, 2423
BspANI	GGCC	2 cut(s)	747, 1566
BspDI	ATCGAT	1 cut(s)	930
BspEI	TCCGGA	1 cut(s)	2411
BspFNI	CGCG	1 cut(s)	59
BspLI	GGNNCC	5 cut(s)	350, 826, 1425, 1567, 1644
BspOI	GCTAGC	1 cut(s)	848
BspPI	GGATC	2 cut(s)	515, 1895
BspQI	GCTCTTC	1 cut(s)	704
BspT104I	TTCGAA	1 cut(s)	29
BspT107I	GGYRCC	1 cut(s)	1423
BsrDI	GCAATG	1 cut(s)	2175
BsrI	ACTGG	3 cut(s)	802, 1499, 2359
BssECI	CCNNGG	6 cut(s)	150, 1222, 1378, 1570, 2050, 2365
BssT1I	CCWWGG	4 cut(s)	150, 1222, 1378, 2050
Bst2UI	CCWGG	4 cut(s)	753, 1572, 1920, 2367
Bst6I	CTCTTC	6 cut(s)	141, 197, 704, 1956, 2141, 2169
BstBAI	YACGTR	2 cut(s)	1406, 2306
BstBI	TTCGAA	1 cut(s)	29
BstC8I	GCNNGC	3 cut(s)	846, 1182, 2087
BstDSI	CCRYGG	2 cut(s)	150, 1222
BstENI	CCTNNNNNAGG	1 cut(s)	726
BstFNI	CGCG	1 cut(s)	59
BstHHI	GCGC	1 cut(s)	2341
BstMAI	GTCTC	6 cut(s)	233, 788, 1403, 1478, 1931, 1971
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 694, 1244, 1796
BstNI	CCWGG	4 cut(s)	753, 1572, 1920, 2367
BstNSI	RCATGY	1 cut(s)	246
BstSCI	CCNGG	4 cut(s)	751, 1570, 1918, 2365
BstSFI	CTRYAG	2 cut(s)	161, 2440
BstUI	CGCG	1 cut(s)	59
BstV1I	GCAGC	7 cut(s)	92, 1252, 1259, 1811, 2359, 2362, 2432
BstV2I	GAAGAC	4 cut(s)	770, 962, 1855, 1862
BstX2I	RGATCY	1 cut(s)	1977
BstYI	RGATCY	1 cut(s)	1977
Bsu15I	ATCGAT	1 cut(s)	930
Bsu36I	CCTNAGG	2 cut(s)	869, 2027
BsuI	GTATCC	2 cut(s)	205, 997
BsuRI	GGCC	2 cut(s)	747, 1566
BsuTUI	ATCGAT	1 cut(s)	930
BtgI	CCRYGG	2 cut(s)	150, 1222
BtgZI	GCGATG	1 cut(s)	1741
BtsIMutI	CAGTG	5 cut(s)	1257, 1506, 1783, 2065, 2253
Cac8I	GCNNGC	3 cut(s)	846, 1182, 2087
CfoI	GCGC	1 cut(s)	2341
Cfr13I	GGNCC	4 cut(s)	746, 824, 1565, 1643
ClaI	ATCGAT	1 cut(s)	930
CseI	GACGC	1 cut(s)	1904
Csp6I	GTAC	2 cut(s)	532, 2044
CviAII	CATG	7 cut(s)	151, 243, 835, 1031, 1174, 1223, 2227
CviQI	GTAC	2 cut(s)	532, 2044
DraIII	CACNNNGTG	1 cut(s)	1662
Eam1104I	CTCTTC	6 cut(s)	141, 197, 704, 1956, 2141, 2169
EarI	CTCTTC	6 cut(s)	141, 197, 704, 1956, 2141, 2169
Ecl136II	GAGCTC	3 cut(s)	676, 1477, 1651
Eco130I	CCWWGG	4 cut(s)	150, 1222, 1378, 2050
Eco24I	GRGCYC	5 cut(s)	678, 1030, 1479, 1653, 2035
Eco47I	GGWCC	2 cut(s)	824, 1643
Eco53kI	GAGCTC	3 cut(s)	676, 1477, 1651
Eco57I	CTGAAG	3 cut(s)	1281, 1380, 2041
Eco72I	CACGTG	1 cut(s)	2306
Eco81I	CCTNAGG	2 cut(s)	869, 2027
Eco88I	CYCGRG	1 cut(s)	827
EcoICRI	GAGCTC	3 cut(s)	676, 1477, 1651
EcoNI	CCTNNNNNAGG	1 cut(s)	726
EcoO109I	RGGNCCY	1 cut(s)	1643
EcoRII	CCWGG	4 cut(s)	751, 1570, 1918, 2365
EcoT14I	CCWWGG	4 cut(s)	150, 1222, 1378, 2050
EcoT22I	ATGCAT	2 cut(s)	415, 1280
EcoT38I	GRGCYC	5 cut(s)	678, 1030, 1479, 1653, 2035
ErhI	CCWWGG	4 cut(s)	150, 1222, 1378, 2050
Esp3I	CGTCTC	1 cut(s)	1931
FaeI	CATG	7 cut(s)	154, 246, 838, 1034, 1177, 1226, 2230
FalI	AAGNNNNNCTT	6 cut(s)	1574, 1606, 1847, 1879, 1976, 2008
FaqI	GGGAC	4 cut(s)	49, 810, 1044, 2362
FatI	CATG	7 cut(s)	150, 242, 834, 1030, 1173, 1222, 2226
FbaI	TGATCA	1 cut(s)	1360
FblI	GTMKAC	1 cut(s)	2394
Fnu4HI	GCNGC	7 cut(s)	81, 1248, 1266, 1800, 2373, 2376, 2421
FriOI	GRGCYC	5 cut(s)	678, 1030, 1479, 1653, 2035
Fsp4HI	GCNGC	7 cut(s)	81, 1248, 1266, 1800, 2373, 2376, 2421
FspBI	CTAG	4 cut(s)	473, 845, 1307, 2238
GlaI	GCGC	1 cut(s)	2340
GluI	GCNGC	7 cut(s)	81, 1248, 1266, 1800, 2373, 2376, 2421
GsaI	CCCAGC	1 cut(s)	994
GsuI	CTGGAG	5 cut(s)	86, 531, 2279, 2376, 2388
HaeIII	GGCC	2 cut(s)	747, 1566
HapII	CCGG	2 cut(s)	449, 2412
HgaI	GACGC	1 cut(s)	1904
HhaI	GCGC	1 cut(s)	2341
Hin1II	CATG	7 cut(s)	154, 246, 838, 1034, 1177, 1226, 2230
Hin6I	GCGC	1 cut(s)	2339
HinP1I	GCGC	1 cut(s)	2339
HincII	GTYRAC	2 cut(s)	2023, 2395
HindII	GTYRAC	2 cut(s)	2023, 2395
HindIII	AAGCTT	1 cut(s)	1178
HpaII	CCGG	2 cut(s)	449, 2412
HphI	GGTGA	7 cut(s)	131, 188, 463, 584, 863, 1530, 1995
Hpy166II	GTNNAC	6 cut(s)	358, 575, 665, 2023, 2046, 2395
Hpy188I	TCNGA	7 cut(s)	25, 293, 657, 1767, 1874, 2111, 2289
Hpy8I	GTNNAC	6 cut(s)	358, 575, 665, 2023, 2046, 2395
Hpy99I	CGWCG	1 cut(s)	131
HpyCH4IV	ACGT	3 cut(s)	1096, 1405, 2305
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 694, 1244, 1796
HpySE526I	ACGT	3 cut(s)	1096, 1405, 2305
Hsp92II	CATG	7 cut(s)	154, 246, 838, 1034, 1177, 1226, 2230
HspAI	GCGC	1 cut(s)	2339
Kpn2I	TCCGGA	1 cut(s)	2411
Ksp22I	TGATCA	1 cut(s)	1360
LguI	GCTCTTC	1 cut(s)	704
Lsp1109I	GCAGC	7 cut(s)	92, 1252, 1259, 1811, 2359, 2362, 2432
LweI	GCATC	7 cut(s)	400, 697, 1315, 1516, 1777, 2245, 2326
MaeI	CTAG	4 cut(s)	473, 845, 1307, 2238
MaeII	ACGT	3 cut(s)	1096, 1405, 2305
MaeIII	GTNAC	5 cut(s)	863, 1382, 1536, 1970, 2210
MflI	RGATCY	1 cut(s)	1977
MhlI	GDGCHC	5 cut(s)	678, 1030, 1479, 1653, 2035
MluCI	AATT	7 cut(s)	258, 558, 1110, 1299, 1332, 1339, 1517
MlyI	GAGTC	7 cut(s)	952, 1560, 1745, 1973, 2165, 2284, 2400
MmeI	TCCRAC	2 cut(s)	585, 1762
Mph1103I	ATGCAT	2 cut(s)	415, 1280
MroI	TCCGGA	1 cut(s)	2411
MseI	TTAA	7 cut(s)	111, 188, 420, 504, 1017, 1983, 2202
MslI	CAYNNNNRTG	1 cut(s)	1227
MspA1I	CMGCKG	1 cut(s)	2423
MspI	CCGG	2 cut(s)	449, 2412
MspR9I	CCNGG	4 cut(s)	753, 1572, 1920, 2367
Mva1269I	GAATGC	2 cut(s)	904, 1852
MvaI	CCWGG	4 cut(s)	753, 1572, 1920, 2367
MvnI	CGCG	1 cut(s)	59
MwoI	GCNNNNNNNGC	4 cut(s)	11, 694, 1244, 1796
NcoI	CCATGG	2 cut(s)	150, 1222
NheI	GCTAGC	1 cut(s)	844
NlaIII	CATG	7 cut(s)	154, 246, 838, 1034, 1177, 1226, 2230
NlaIV	GGNNCC	5 cut(s)	350, 826, 1425, 1567, 1644
NmuCI	GTSAC	2 cut(s)	1536, 2210
NsiI	ATGCAT	2 cut(s)	415, 1280
NspI	RCATGY	1 cut(s)	246
NspV	TTCGAA	1 cut(s)	29
PciI	ACATGT	1 cut(s)	242
PciSI	GCTCTTC	1 cut(s)	704
PcsI	WCGNNNNNNNCGW	1 cut(s)	35
PctI	GAATGC	2 cut(s)	904, 1852
PkrI	GCNGC	7 cut(s)	82, 1249, 1267, 1801, 2374, 2377, 2422
PleI	GAGTC	7 cut(s)	952, 1559, 1745, 1972, 2164, 2284, 2399
PmaCI	CACGTG	1 cut(s)	2306
PmlI	CACGTG	1 cut(s)	2306
PpsI	GAGTC	7 cut(s)	952, 1559, 1745, 1972, 2164, 2284, 2399
Ppu21I	YACGTR	2 cut(s)	1406, 2306
PpuMI	RGGWCCY	1 cut(s)	1643
PscI	ACATGT	1 cut(s)	242
Psp124BI	GAGCTC	3 cut(s)	678, 1479, 1653
Psp5II	RGGWCCY	1 cut(s)	1643
Psp6I	CCWGG	4 cut(s)	751, 1570, 1918, 2365
PspCI	CACGTG	1 cut(s)	2306
PspFI	CCCAGC	1 cut(s)	990
PspGI	CCWGG	4 cut(s)	751, 1570, 1918, 2365
PspN4I	GGNNCC	5 cut(s)	350, 826, 1425, 1567, 1644
PspPI	GGNCC	4 cut(s)	746, 824, 1565, 1643
PspPPI	RGGWCCY	1 cut(s)	1643
PsuI	RGATCY	1 cut(s)	1977
RsaI	GTAC	2 cut(s)	533, 2045
RsaNI	GTAC	2 cut(s)	532, 2044
RseI	CAYNNNNRTG	1 cut(s)	1227
SacI	GAGCTC	3 cut(s)	678, 1479, 1653
SalI	GTCGAC	1 cut(s)	2393
SapI	GCTCTTC	1 cut(s)	704
SaqAI	TTAA	7 cut(s)	111, 188, 420, 504, 1017, 1983, 2202
SatI	GCNGC	7 cut(s)	81, 1248, 1266, 1800, 2373, 2376, 2421
Sau96I	GGNCC	4 cut(s)	746, 824, 1565, 1643
ScaI	AGTACT	1 cut(s)	533
SchI	GAGTC	7 cut(s)	952, 1560, 1745, 1973, 2165, 2284, 2400
ScrFI	CCNGG	4 cut(s)	753, 1572, 1920, 2367
SduI	GDGCHC	5 cut(s)	678, 1030, 1479, 1653, 2035
SfaNI	GCATC	7 cut(s)	400, 697, 1315, 1516, 1777, 2245, 2326
SfcI	CTRYAG	2 cut(s)	161, 2440
SfuI	TTCGAA	1 cut(s)	29
SinI	GGWCC	2 cut(s)	824, 1643
SmiMI	CAYNNNNRTG	1 cut(s)	1227
SmlI	CTYRAG	2 cut(s)	1457, 1993
SmoI	CTYRAG	2 cut(s)	1457, 1993
Sse9I	AATT	7 cut(s)	258, 558, 1110, 1299, 1332, 1339, 1517
SsiI	CCGC	4 cut(s)	57, 1184, 2066, 2423
SspMI	CTAG	4 cut(s)	473, 845, 1307, 2238
SstI	GAGCTC	3 cut(s)	678, 1479, 1653
StyD4I	CCNGG	4 cut(s)	751, 1570, 1918, 2365
StyI	CCWWGG	4 cut(s)	150, 1222, 1378, 2050
TaiI	ACGT	3 cut(s)	1099, 1408, 2308
TasI	AATT	7 cut(s)	258, 558, 1110, 1299, 1332, 1339, 1517
TatI	WGTACW	2 cut(s)	531, 2043
Tru1I	TTAA	7 cut(s)	111, 188, 420, 504, 1017, 1983, 2202
Tru9I	TTAA	7 cut(s)	111, 188, 420, 504, 1017, 1983, 2202
TscAI	CASTG	5 cut(s)	1264, 1506, 1783, 2065, 2253
TseFI	GTSAC	2 cut(s)	1536, 2210
TseI	GCWGC	7 cut(s)	80, 1247, 1265, 1799, 2372, 2375, 2420
Tsp45I	GTSAC	2 cut(s)	1536, 2210
TspGWI	ACGGA	4 cut(s)	115, 978, 994, 1844
TspRI	CASTG	5 cut(s)	1264, 1506, 1783, 2065, 2253
VpaK11BI	GGWCC	2 cut(s)	824, 1643
XagI	CCTNNNNNAGG	1 cut(s)	726
XapI	RAATTY	3 cut(s)	558, 1332, 1517
XceI	RCATGY	1 cut(s)	246
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1229, 1376, 2057, 2303
XmiI	GTMKAC	1 cut(s)	2394
XspI	CTAG	4 cut(s)	473, 845, 1307, 2238
ZrmI	AGTACT	1 cut(s)	533
Zsp2I	ATGCAT	2 cut(s)	415, 1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.