Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001165

Physical Location & Seq

Genomic Coordinates Reverse (-)

11857189 .. 11862485

Gene Structure 5297 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1125700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2415 bp

ATGGCTCACCAAGCCGAATCGTCCGACTCGAAGAAGAAGGACTTCTCGACGGCAATTCTGGAGCGGAAGAAGTCGCCGAACCGTCTTGTCGTTGATGAGGCTGTAAACGACGATAACTCCGTCGTTTCAATGCATCCCGAAACCATGGAAAAGCTACAGCTTTTCCGTGGCGATACCATCCTCATCAAGGGGAAGAAACGAAAAGACACAATCTGCATTGCACTTGCTGATGACACCTGTGAAGAGCCAAGAATCAGGATGAACAAGGTTGTCAGGTCGAATTTAAGGGTTCGACTTGGGGATGTTGTGTCTGTACATCAATGCCCTGATGTTAAGTATGGGAAGCGTGTCCACATTCTGCCTATTGATGACTCTATAGAAGGAGTTACCGGAAATCTCTTTGATGCCTTTTTGAAACCTTATTTCTTGGAAGCTTACCGCCCTGTGAGGAAAGGAGATTTGTTCCTTGTCAGAGGAGGAATGAGAAGTGTAGAGTTTAAGGTCATTGAAACTGACCCAGGAGAATATTGTGTGGTTGCCCCTGACACTGAGATCTTTTGTGAGGGGGAACCTGTGAGAAGGGAGGATGAAGAAAGGTTGGATGAAGTTGGTTATGATGATGTGGGTGGTGTTAGGAAGCAAATGGCTCAAATCCGTGAACTAGTAGAGCTGCCACTAAGGCATCCTCAACTTTTCAAATCAATTGGTGTGAAACCTCCGAAAGGAATTCTACTTTATGGACCCCCGGGTTCAGGAAAGACATTAATAGCCAGAGCTGTTGCCAATGAAACTGGTGCTTTTTTCTTTTGCATTAATGGGCCAGAGATTATGTCAAAATTGGCTGGAGAGAGTGAGAGCAATCTCAGAAAGGCATTTGAAGAAGCGGAGAAGAATGCCCCATCCATCATCTTTATTGATGAGCTTGATTCAATTGCTCCTAAGCGGGAGAAGACACATGGTGAGGTTGAAAGGCGGATTGTCTCTCAGCTCTTGACTCTGATGGATGGACTGAAATCTCGAGCCCATGTTATTGTTATTGGTGCTACAAATCGTCCAAACAGTATTGATGCGGCTTTGAGAAGGTTTGGTAGGTTTGATAGGGAGATAGACATTGGTGTTCCTGATGAAGTTGGGCGCCTTGAAGTTCTTCGCATACACACAAAGAACATGAAACTCTCTGATGATGTTGATCTAGAGAGGATTTCCAAGGATACTCATGGGTATGTTGGTGCTGACCTTGCTGCTCTCTGCACTGAAGCTGCTCTGCAATGCATTAGGGAGAAGATGGACGTGATTGACTTGGAAGATGAATCCATCGATGCGGAGATACTGAATTCAATGGCGGTTACAAATGAGCACTTCCAAACTGCTCTTGGAACAAGCAATCCATCTGCTTTGCGTGAAACAGTTGTTGAAGTTCCTAATGTAAGTTGGGAGGATATTGGAGGCCTTGAGAATGTTAAGCGAGAGTTACAAGAGACTGTTCAATATCCTGTTGAGCACCCGGAGAAGTTTGAGAAATTTGGCATGTCACCTTCTAAAGGGGTGCTTTTCTACGGGCCCCCAGGATGTGGGAAAACTCTTTTGGCCAAGGCAATTGCAAATGAATGTCAAGCTAACTTCATCAGTATTAAAGGCCCCGAACTTCTCACCATGTGGTTTGGTGAGAGTGAAGCCAATGTGCGAGAAATCTTTGACAAGGCTCGTGCATCTGCACCATGTGTCCTTTTCTTTGATGAGCTTGATTCCATTGCTACCCAGAGGGGAGGCAGTTCTGGGGATGCAGGTGGTGCTGCTGATCGTGTTCTTAACCAACTCCTGACTGAAATGGATGGCATGACAGCAAAAAAGACTGTTTTCATTATTGGGGCCACAAACCGACCTGACATTATTGACCCGGCACTTCTGCGCCCTGGCCGGCTTGACCAGTTGATCTACATTCCTCTTCCAGATGAAGAATCACGTTTCCAAATCTTTAAATCTTGCTTGAGAAAATCACCTGTTTCAAAGGATGTGGATTTAAGAGCACTGGCCAGGTATACACAGGGCTTTAGTGGGGCTGATATCACCGAGATCTGCCAGCGGTCATGCAAGTATGCCATAAGAGAGAATATAGAAAAGGATATCGAGCGGGAGAGGAGGAGAGCAGAGAATCCAGATTCTATGGATGAGGACATTGACGATGAAGTGGCGGAGATTAAAGCTGCTCATTTTGAGGAGTCGATGAAATATGCTCGTAGGAGTGTCAGCGATGCTGATATACGCAAATACCAGGTCTTTGCTCAGACCTTGCAGCAGTCCAGGGGTTTCGGAACTGAGTTTAAGTTTACTGAAAATCCACAAACTGGGACGACCGGCTCTGATGCATTTGCAACTTCTGCTGGTGCTGCCGATGAAGACGATTTGTATAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010038 GO:0010604 GO:0012505 GO:0016020 GO:0019222 GO:0031974 GO:0031981 GO:0042176 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046686 GO:0048518 GO:0050789 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0070013 GO:0071944 GO:0080090

KEGG Pathways

Metabolic & Signaling

ko04141 ko05134 map04141 map05134

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

805

Amino Acids

89.59

Weight (kDa)

5.11

Isoelectric Point (pI)

42.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_N	PF02359	28 - 109	2.4e-22	Cell division protein 48 (CDC48), N-terminal domain
CDC48_2	PF02933	129 - 193	1e-10	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	243 - 372	1.9e-47	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	395 - 436	7.1e-16	AAA+ lid domain
AAA	PF00004	516 - 649	1.4e-47	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	671 - 710	1.3e-11	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53230
fragaria_vesca	FvH4_2g06940 FvH4_2g06950 FvH4_2g07260 FvH4_4g17680 FvH4_6g16220 FvH4_6g16220
malus_domestica	MD03G1125700.v1.1 MD04G1094400.v1.1 MD11G1142500.v1.1 MD12G1114300.v1.1 MD17G1225300.v1.1 MD17G1225400.v1.1 MD17G1225600.v1.1 MD17G1228800.v1.1
prunus_persica	Prupe.4G237600_v2.0.a1 Prupe.6G107800_v2.0.a1 Prupe.6G233700_v2.0.a1 Prupe.6G233700_v2.0.a1
pyrus_communis	pycom04g08940 pycom04g08950 pycom09g14580 pycom11g11820 pycom12g11360
rosa_chinensis	RchiOBHm_Chr3g0470061 RchiOBHm_Chr4g0421621 RchiOBHm_Chr6g0259281 RchiOBHm_Chr6g0259871
rosa_laevigata	RLG00000007663 RLG00000014503 RLG00000014543 RLG00000024300
rosa_multiflora	Rmu_co8522155.1_g000003 Rmu_sc0005600.1_g000001 Rmu_sc0009049.1_g000005 Rmu_sc0011974.1_g000005 Rmu_sc0033604.1_g000001 Rmu_sc0033605.1_g000001 Rmu_ssc0000050.1_g000089
rosa_roxburghii	Rroxscaffold_5G00363870 Rroxscaffold_6G00411220
rosa_rugosa	Rorug03G0106600 Rorug04G0173900 Rorug05G0586800 Rorug05G0590900
rosa_samantha	Rh3AG155800 Rh4CG248200 Rh6AG103300 Rh6AG103400 Rh6AG107600 Rh6BG095700 Rh6CG092600 Rh6CG096400
rosa_wichuraiana	Rw3G014670 Rw4G020040 Rw6G008920 Rw6G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1775
Acc36I	ACCTGC	1 cut(s)	1775
AccB1I	GGYRCC	1 cut(s)	1134
AccB7I	CCANNNNNTGG	2 cut(s)	1571, 2345
AccBSI	CCGCTC	2 cut(s)	64, 2131
AccI	GTMKAC	1 cut(s)	2039
AcoI	YGGCCR	3 cut(s)	1587, 1915, 2031
AcsI	RAATTY	4 cut(s)	280, 726, 1333, 1520
AcuI	CTGAAG	1 cut(s)	1275
AcyI	GRCGYC	1 cut(s)	1135
AdeI	CACNNNGTG	3 cut(s)	959, 1656, 1721
AfaI	GTAC	1 cut(s)	315
AfiI	CCNNNNNNNGG	6 cut(s)	187, 722, 752, 1541, 1571, 2345
AhlI	ACTAGT	1 cut(s)	661
AjiI	CACGTC	1 cut(s)	1291
AjnI	CCWGG	6 cut(s)	517, 1564, 1912, 2033, 2271, 2300
AjuI	GAANNNNNNNTTGG	2 cut(s)	1568, 1600
AloI	GAACNNNNNNTCC	2 cut(s)	1369, 1401
Alw21I	GWGCWC	3 cut(s)	1359, 1503, 2029
Alw26I	GTCTC	2 cut(s)	985, 1472
Ama87I	CYCGRG	2 cut(s)	745, 1017
AoxI	GGCC	8 cut(s)	818, 1447, 1559, 1587, 1636, 1869, 1915, 2031
ApaI	GGGCCC	1 cut(s)	1563
ApeKI	GCWGC	7 cut(s)	670, 1241, 1259, 1793, 2204, 2293, 2387
ApoI	RAATTY	4 cut(s)	280, 726, 1333, 1520
ArsI	GACNNNNNNTTYG	2 cut(s)	2259, 2291
AseI	ATTAAT	2 cut(s)	764, 813
Asp700I	GAANNNNTTC	2 cut(s)	41, 1146
AspLEI	GCGC	2 cut(s)	1137, 1911
AspS9I	GGNCC	6 cut(s)	740, 818, 1559, 1560, 1637, 1869
AsuC2I	CCSGG	4 cut(s)	746, 747, 1505, 1898
AsuHPI	GGTGA	6 cut(s)	971, 1524, 1642, 1676, 1989, 2059
AvaI	CYCGRG	2 cut(s)	745, 1017
AvaII	GGWCC	1 cut(s)	740
BaeGI	GKGCMC	1 cut(s)	1563
BalI	TGGCCA	2 cut(s)	1589, 2033
BanI	GGYRCC	1 cut(s)	1134
BanII	GRGCYC	2 cut(s)	1024, 1563
BauI	CACGAG	1 cut(s)	1704
BbsI	GAAGAC	2 cut(s)	956, 2403
Bbv12I	GWGCWC	3 cut(s)	1359, 1503, 2029
BbvI	GCAGC	7 cut(s)	657, 1228, 1246, 1780, 2191, 2305, 2374
BccI	CCATC	9 cut(s)	185, 907, 911, 994, 998, 1279, 1322, 1396, 1826
BceAI	ACGGC	1 cut(s)	66
BciT130I	CCWGG	6 cut(s)	519, 1566, 1914, 2035, 2273, 2302
BciVI	GTATCC	1 cut(s)	1204
BcnI	CCSGG	4 cut(s)	746, 747, 1505, 1898
BcoDI	GTCTC	2 cut(s)	985, 1472
BcuI	ACTAGT	1 cut(s)	661
BfaI	CTAG	2 cut(s)	662, 1193
BfmI	CTRYAG	2 cut(s)	155, 375
BfoI	RGCGCY	1 cut(s)	1138
BfuAI	ACCTGC	1 cut(s)	1775
BfuI	GTATCC	1 cut(s)	1204
BglI	GCCNNNNNGGC	1 cut(s)	679
BglII	AGATCT	2 cut(s)	552, 2073
BisI	GCNGC	8 cut(s)	671, 1071, 1242, 1260, 1794, 2205, 2294, 2388
BlsI	GCNGC	8 cut(s)	672, 1072, 1243, 1261, 1795, 2206, 2295, 2389
Bme18I	GGWCC	1 cut(s)	740
BmeT110I	CYCGRG	2 cut(s)	745, 1017
BmgBI	CACGTC	1 cut(s)	1291
BmgT120I	GGNCC	6 cut(s)	740, 818, 1559, 1560, 1637, 1869
BmiI	GGNNCC	7 cut(s)	570, 742, 1136, 1561, 1562, 1639, 1870
BmrI	ACTGGG	1 cut(s)	2355
BmsI	GCATC	9 cut(s)	142, 394, 691, 1057, 1309, 1718, 1771, 2242, 2353
BmuI	ACTGGG	1 cut(s)	2355
BpiI	GAAGAC	2 cut(s)	956, 2403
BpmI	CTGGAG	2 cut(s)	80, 864
Bpu10I	CCTNAGC	1 cut(s)	939
BpuEI	CTTGAG	2 cut(s)	1472, 2008
BpuMI	CCSGG	4 cut(s)	746, 747, 1505, 1898
Bsa29I	ATCGAT	1 cut(s)	1317
BsaBI	GATNNNNATC	1 cut(s)	1188
BsaHI	GRCGYC	1 cut(s)	1135
BsaWI	WCCGGW	1 cut(s)	389
BsaXI	ACNNNNNCTCC	2 cut(s)	101, 131
Bsc4I	CCNNNNNNNGG	6 cut(s)	187, 722, 752, 1541, 1571, 2345
Bse118I	RCCGGY	2 cut(s)	1917, 2354
Bse1I	ACTGG	4 cut(s)	796, 1927, 2034, 2350
Bse3DI	GCAATG	3 cut(s)	216, 1274, 1749
Bse8I	GATNNNNATC	1 cut(s)	1188
BseBI	CCWGG	6 cut(s)	519, 1566, 1914, 2035, 2273, 2302
BseCI	ATCGAT	1 cut(s)	1317
BseJI	GATNNNNATC	1 cut(s)	1188
BseLI	CCNNNNNNNGG	6 cut(s)	187, 722, 752, 1541, 1571, 2345
BseMI	GCAATG	3 cut(s)	216, 1274, 1749
BseMII	CTCAG	5 cut(s)	540, 877, 998, 2297, 2307
BseNI	ACTGG	4 cut(s)	796, 1927, 2034, 2350
BseRI	GAGGAG	4 cut(s)	489, 2152, 2155, 2231
BseSI	GKGCMC	1 cut(s)	1563
BseXI	GCAGC	7 cut(s)	657, 1228, 1246, 1780, 2191, 2305, 2374
BsgI	GTGCAG	2 cut(s)	1234, 1698
Bsh1285I	CGRYCG	1 cut(s)	2355
BshFI	GGCC	8 cut(s)	820, 1449, 1561, 1589, 1638, 1871, 1917, 2033
BshNI	GGYRCC	1 cut(s)	1134
BshVI	ATCGAT	1 cut(s)	1317
BsiEI	CGRYCG	1 cut(s)	2355
BsiHKAI	GWGCWC	3 cut(s)	1359, 1503, 2029
BsiHKCI	CYCGRG	2 cut(s)	745, 1017
BsiSI	CCGG	6 cut(s)	390, 746, 1505, 1898, 1918, 2355
BslFI	GGGAC	1 cut(s)	2362
BslI	CCNNNNNNNGG	6 cut(s)	187, 722, 752, 1541, 1571, 2345
BsmAI	GTCTC	2 cut(s)	985, 1472
BsmFI	GGGAC	1 cut(s)	2362
BsmI	GAATGC	1 cut(s)	898
BsnI	GGCC	8 cut(s)	820, 1449, 1561, 1589, 1638, 1871, 1917, 2033
BsoBI	CYCGRG	2 cut(s)	745, 1017
Bsp120I	GGGCCC	1 cut(s)	1559
Bsp1286I	GDGCHC	5 cut(s)	1024, 1359, 1503, 1563, 2029
Bsp1407I	TGTACA	1 cut(s)	313
Bsp143I	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
Bsp19I	CCATGG	1 cut(s)	144
BspANI	GGCC	8 cut(s)	820, 1449, 1561, 1589, 1638, 1871, 1917, 2033
BspCNI	CTCAG	5 cut(s)	541, 876, 997, 2296, 2308
BspDI	ATCGAT	1 cut(s)	1317
BspLI	GGNNCC	7 cut(s)	570, 742, 1136, 1561, 1562, 1639, 1870
BspMI	ACCTGC	1 cut(s)	1775
BspQI	GCTCTTC	1 cut(s)	237
BspT107I	GGYRCC	1 cut(s)	1134
BsrBI	CCGCTC	2 cut(s)	64, 2131
BsrDI	GCAATG	3 cut(s)	216, 1274, 1749
BsrFI	RCCGGY	2 cut(s)	1917, 2354
BsrGI	TGTACA	1 cut(s)	313
BsrI	ACTGG	4 cut(s)	796, 1927, 2034, 2350
BssAI	RCCGGY	2 cut(s)	1917, 2354
BssMI	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
BssNAI	GTATAC	1 cut(s)	2040
BssNI	GRCGYC	1 cut(s)	1135
BssSI	CACGAG	1 cut(s)	1704
BssT1I	CCWWGG	3 cut(s)	144, 1206, 1590
Bst1107I	GTATAC	1 cut(s)	2040
Bst2BI	CACGAG	1 cut(s)	1704
Bst2UI	CCWGG	6 cut(s)	519, 1566, 1914, 2035, 2273, 2302
Bst4CI	ACNGT	5 cut(s)	83, 1061, 1408, 1483, 1855
Bst6I	CTCTTC	2 cut(s)	237, 1950
BstACI	GRCGYC	1 cut(s)	1135
BstAPI	GCANNNNNTGC	2 cut(s)	1790, 2378
BstAUI	TGTACA	1 cut(s)	313
BstC8I	GCNNGC	2 cut(s)	1919, 2081
BstDEI	CTNAG	7 cut(s)	549, 677, 863, 939, 984, 2283, 2316
BstDSI	CCRYGG	2 cut(s)	144, 166
BstENI	CCTNNNNNAGG	3 cut(s)	185, 720, 1539
BstH2I	RGCGCY	1 cut(s)	1138
BstHHI	GCGC	2 cut(s)	1137, 1911
BstKTI	GATC	5 cut(s)	555, 1192, 1801, 1935, 2076
BstMAI	GTCTC	2 cut(s)	985, 1472
BstMBI	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
BstMCI	CGRYCG	1 cut(s)	2355
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 679, 1238, 1790, 2378, 2387
BstNI	CCWGG	6 cut(s)	519, 1566, 1914, 2035, 2273, 2302
BstNSI	RCATGY	1 cut(s)	1531
BstSFI	CTRYAG	2 cut(s)	155, 375
BstSLI	GKGCMC	1 cut(s)	1563
BstV1I	GCAGC	7 cut(s)	657, 1228, 1246, 1780, 2191, 2305, 2374
BstV2I	GAAGAC	2 cut(s)	956, 2403
BstX2I	RGATCY	2 cut(s)	552, 2073
BstYI	RGATCY	2 cut(s)	552, 2073
BstZ17I	GTATAC	1 cut(s)	2040
Bsu15I	ATCGAT	1 cut(s)	1317
BsuI	GTATCC	1 cut(s)	1204
BsuRI	GGCC	8 cut(s)	820, 1449, 1561, 1589, 1638, 1871, 1917, 2033
BsuTUI	ATCGAT	1 cut(s)	1317
BtgI	CCRYGG	2 cut(s)	144, 166
BtgZI	GCGATG	1 cut(s)	2265
BtrI	CACGTC	1 cut(s)	1291
BtsIMutI	CAGTG	3 cut(s)	546, 1251, 2027
BveI	ACCTGC	1 cut(s)	1775
Cac8I	GCNNGC	2 cut(s)	1919, 2081
CfoI	GCGC	2 cut(s)	1137, 1911
Cfr10I	RCCGGY	2 cut(s)	1917, 2354
Cfr13I	GGNCC	6 cut(s)	740, 818, 1559, 1560, 1637, 1869
Cfr9I	CCCGGG	1 cut(s)	745
ClaI	ATCGAT	1 cut(s)	1317
CsiI	ACCWGGT	1 cut(s)	2271
Csp6I	GTAC	1 cut(s)	314
CspCI	CAANNNNNGTGG	2 cut(s)	1995, 2030
CviQI	GTAC	1 cut(s)	314
DdeI	CTNAG	7 cut(s)	549, 677, 863, 939, 984, 2283, 2316
DinI	GGCGCC	1 cut(s)	1136
DpnI	GATC	5 cut(s)	554, 1191, 1800, 1934, 2075
DpnII	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
DraI	TTTAAA	1 cut(s)	1978
DraIII	CACNNNGTG	3 cut(s)	959, 1656, 1721
EaeI	YGGCCR	3 cut(s)	1587, 1915, 2031
Eam1104I	CTCTTC	2 cut(s)	237, 1950
EarI	CTCTTC	2 cut(s)	237, 1950
EciI	GGCGGA	2 cut(s)	988, 2207
Eco130I	CCWWGG	3 cut(s)	144, 1206, 1590
Eco147I	AGGCCT	1 cut(s)	1449
Eco24I	GRGCYC	2 cut(s)	1024, 1563
Eco32I	GATATC	2 cut(s)	2065, 2125
Eco47I	GGWCC	1 cut(s)	740
Eco57I	CTGAAG	1 cut(s)	1275
Eco88I	CYCGRG	2 cut(s)	745, 1017
EcoNI	CCTNNNNNAGG	3 cut(s)	185, 720, 1539
EcoO109I	RGGNCCY	2 cut(s)	1560, 1637
EcoRI	GAATTC	2 cut(s)	726, 1333
EcoRII	CCWGG	6 cut(s)	517, 1564, 1912, 2033, 2271, 2300
EcoRV	GATATC	2 cut(s)	2065, 2125
EcoT14I	CCWWGG	3 cut(s)	144, 1206, 1590
EcoT22I	ATGCAT	3 cut(s)	135, 1274, 2368
EcoT38I	GRGCYC	2 cut(s)	1024, 1563
EgeI	GGCGCC	1 cut(s)	1136
EheI	GGCGCC	1 cut(s)	1136
ErhI	CCWWGG	3 cut(s)	144, 1206, 1590
FalI	AAGNNNNNCTT	4 cut(s)	26, 58, 1533, 1565
FaqI	GGGAC	1 cut(s)	2362
FauI	CCCGC	2 cut(s)	936, 2124
FblI	GTMKAC	1 cut(s)	2039
Fnu4HI	GCNGC	8 cut(s)	671, 1071, 1242, 1260, 1794, 2205, 2294, 2388
FriOI	GRGCYC	2 cut(s)	1024, 1563
Fsp4HI	GCNGC	8 cut(s)	671, 1071, 1242, 1260, 1794, 2205, 2294, 2388
FspBI	CTAG	2 cut(s)	662, 1193
GlaI	GCGC	2 cut(s)	1136, 1910
GluI	GCNGC	8 cut(s)	671, 1071, 1242, 1260, 1794, 2205, 2294, 2388
GsuI	CTGGAG	2 cut(s)	80, 864
HaeII	RGCGCY	1 cut(s)	1138
HaeIII	GGCC	8 cut(s)	820, 1449, 1561, 1589, 1638, 1871, 1917, 2033
HapII	CCGG	6 cut(s)	390, 746, 1505, 1898, 1918, 2355
HhaI	GCGC	2 cut(s)	1137, 1911
Hin1I	GRCGYC	1 cut(s)	1135
Hin6I	GCGC	2 cut(s)	1135, 1909
HinP1I	GCGC	2 cut(s)	1135, 1909
HindIII	AAGCTT	1 cut(s)	432
HpaII	CCGG	6 cut(s)	390, 746, 1505, 1898, 1918, 2355
HphI	GGTGA	6 cut(s)	971, 1524, 1642, 1676, 1989, 2059
Hpy166II	GTNNAC	5 cut(s)	106, 352, 659, 2040, 2328
Hpy188I	TCNGA	9 cut(s)	25, 473, 720, 866, 999, 1180, 2286, 2312, 2362
Hpy8I	GTNNAC	5 cut(s)	106, 352, 659, 2040, 2328
Hpy99I	CGWCG	3 cut(s)	52, 113, 125
HpyAV	CCTTC	5 cut(s)	31, 374, 573, 1074, 1545
HpyCH4III	ACNGT	5 cut(s)	83, 1061, 1408, 1483, 1855
HpyCH4IV	ACGT	2 cut(s)	1290, 1963
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 679, 1238, 1790, 2378, 2387
HpyF3I	CTNAG	7 cut(s)	549, 677, 863, 939, 984, 2283, 2316
HpySE526I	ACGT	2 cut(s)	1290, 1963
Hsp92I	GRCGYC	1 cut(s)	1135
HspAI	GCGC	2 cut(s)	1135, 1909
KasI	GGCGCC	1 cut(s)	1134
KroI	GCCGGC	1 cut(s)	1917
KroNI	GCCGGC	1 cut(s)	1919
Kzo9I	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
LguI	GCTCTTC	1 cut(s)	237
LmnI	GCTCC	2 cut(s)	61, 940
Lsp1109I	GCAGC	7 cut(s)	657, 1228, 1246, 1780, 2191, 2305, 2374
LweI	GCATC	9 cut(s)	142, 394, 691, 1057, 1309, 1718, 1771, 2242, 2353
MabI	ACCWGGT	1 cut(s)	2271
MaeI	CTAG	2 cut(s)	662, 1193
MaeII	ACGT	2 cut(s)	1290, 1963
MaeIII	GTNAC	4 cut(s)	385, 1345, 1470, 1530
MalI	GATC	5 cut(s)	554, 1191, 1800, 1934, 2075
MbiI	CCGCTC	2 cut(s)	64, 2131
MboI	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
MfeI	CAATTG	3 cut(s)	702, 930, 1596
MflI	RGATCY	2 cut(s)	552, 2073
MhlI	GDGCHC	5 cut(s)	1024, 1359, 1503, 1563, 2029
MlsI	TGGCCA	2 cut(s)	1589, 2033
MluCI	AATT	9 cut(s)	54, 280, 702, 726, 836, 930, 1333, 1520, 1596
MluNI	TGGCCA	2 cut(s)	1589, 2033
Mly113I	GGCGCC	1 cut(s)	1135
MlyI	GAGTC	4 cut(s)	20, 365, 988, 2228
MmeI	TCCRAC	2 cut(s)	48, 579
Mox20I	TGGCCA	2 cut(s)	1589, 2033
Mph1103I	ATGCAT	3 cut(s)	135, 1274, 2368
MroNI	GCCGGC	1 cut(s)	1917
MroXI	GAANNNNTTC	2 cut(s)	41, 1146
MscI	TGGCCA	2 cut(s)	1589, 2033
MslI	CAYNNNNRTG	1 cut(s)	1221
Msp20I	TGGCCA	2 cut(s)	1589, 2033
MspA1I	CMGCKG	1 cut(s)	2083
MspI	CCGG	6 cut(s)	390, 746, 1505, 1898, 1918, 2355
MunI	CAATTG	3 cut(s)	702, 930, 1596
Mva1269I	GAATGC	1 cut(s)	898
MvaI	CCWGG	6 cut(s)	519, 1566, 1914, 2035, 2273, 2302
MwoI	GCNNNNNNNGC	6 cut(s)	11, 679, 1238, 1790, 2378, 2387
NaeI	GCCGGC	1 cut(s)	1919
NarI	GGCGCC	1 cut(s)	1135
NciI	CCSGG	4 cut(s)	746, 747, 1505, 1898
NcoI	CCATGG	1 cut(s)	144
NdeII	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
NgoMIV	GCCGGC	1 cut(s)	1917
NlaIV	GGNNCC	7 cut(s)	570, 742, 1136, 1561, 1562, 1639, 1870
NmuCI	GTSAC	1 cut(s)	1530
NsiI	ATGCAT	3 cut(s)	135, 1274, 2368
NspI	RCATGY	1 cut(s)	1531
PaeR7I	CTCGAG	1 cut(s)	1017
PaqCI	CACCTGC	1 cut(s)	1775
PceI	AGGCCT	1 cut(s)	1449
PciSI	GCTCTTC	1 cut(s)	237
PcsI	WCGNNNNNNNCGW	2 cut(s)	26, 117
PctI	GAATGC	1 cut(s)	898
PdiI	GCCGGC	1 cut(s)	1919
PdmI	GAANNNNTTC	2 cut(s)	41, 1146
PfeI	GAWTC	8 cut(s)	17, 252, 926, 1310, 1745, 1958, 2152, 2159
PflMI	CCANNNNNTGG	2 cut(s)	1571, 2345
PkrI	GCNGC	8 cut(s)	672, 1072, 1243, 1261, 1795, 2206, 2295, 2389
PleI	GAGTC	4 cut(s)	20, 365, 988, 2227
PluTI	GGCGCC	1 cut(s)	1138
PpsI	GAGTC	4 cut(s)	20, 365, 988, 2227
PshBI	ATTAAT	2 cut(s)	764, 813
Psp6I	CCWGG	6 cut(s)	517, 1564, 1912, 2033, 2271, 2300
PspGI	CCWGG	6 cut(s)	517, 1564, 1912, 2033, 2271, 2300
PspN4I	GGNNCC	7 cut(s)	570, 742, 1136, 1561, 1562, 1639, 1870
PspOMI	GGGCCC	1 cut(s)	1559
PspPI	GGNCC	6 cut(s)	740, 818, 1559, 1560, 1637, 1869
PsuI	RGATCY	2 cut(s)	552, 2073
RsaI	GTAC	1 cut(s)	315
RsaNI	GTAC	1 cut(s)	314
RseI	CAYNNNNRTG	1 cut(s)	1221
SapI	GCTCTTC	1 cut(s)	237
SatI	GCNGC	8 cut(s)	671, 1071, 1242, 1260, 1794, 2205, 2294, 2388
Sau3AI	GATC	5 cut(s)	552, 1189, 1798, 1932, 2073
Sau96I	GGNCC	6 cut(s)	740, 818, 1559, 1560, 1637, 1869
SchI	GAGTC	4 cut(s)	20, 365, 988, 2228
SduI	GDGCHC	5 cut(s)	1024, 1359, 1503, 1563, 2029
SexAI	ACCWGGT	1 cut(s)	2271
SfaNI	GCATC	9 cut(s)	142, 394, 691, 1057, 1309, 1718, 1771, 2242, 2353
SfcI	CTRYAG	2 cut(s)	155, 375
SfoI	GGCGCC	1 cut(s)	1136
Sfr274I	CTCGAG	1 cut(s)	1017
SinI	GGWCC	1 cut(s)	740
SlaI	CTCGAG	1 cut(s)	1017
SmaI	CCCGGG	1 cut(s)	747
SmiMI	CAYNNNNRTG	1 cut(s)	1221
SmlI	CTYRAG	3 cut(s)	1017, 1451, 1987
SmoI	CTYRAG	3 cut(s)	1017, 1451, 1987
SpeI	ACTAGT	1 cut(s)	661
Sse9I	AATT	9 cut(s)	54, 280, 702, 726, 836, 930, 1333, 1520, 1596
SseBI	AGGCCT	1 cut(s)	1449
SspDI	GGCGCC	1 cut(s)	1134
SspI	AATATT	1 cut(s)	527
SspMI	CTAG	2 cut(s)	662, 1193
StuI	AGGCCT	1 cut(s)	1449
StyI	CCWWGG	3 cut(s)	144, 1206, 1590
TaaI	ACNGT	5 cut(s)	83, 1061, 1408, 1483, 1855
TaiI	ACGT	2 cut(s)	1293, 1966
TaqI	TCGA	8 cut(s)	29, 47, 278, 292, 1018, 1317, 2127, 2222
TasI	AATT	9 cut(s)	54, 280, 702, 726, 836, 930, 1333, 1520, 1596
TatI	WGTACW	1 cut(s)	313
TauI	GCSGC	1 cut(s)	1073
TfiI	GAWTC	8 cut(s)	17, 252, 926, 1310, 1745, 1958, 2152, 2159
TscAI	CASTG	3 cut(s)	553, 1258, 2034
TseFI	GTSAC	1 cut(s)	1530
TseI	GCWGC	7 cut(s)	670, 1241, 1259, 1793, 2204, 2293, 2387
Tsp45I	GTSAC	1 cut(s)	1530
TspGWI	ACGGA	3 cut(s)	109, 155, 644
TspMI	CCCGGG	1 cut(s)	745
TspRI	CASTG	3 cut(s)	553, 1258, 2034
Van91I	CCANNNNNTGG	2 cut(s)	1571, 2345
VpaK11BI	GGWCC	1 cut(s)	740
VspI	ATTAAT	2 cut(s)	764, 813
XagI	CCTNNNNNAGG	3 cut(s)	185, 720, 1539
XapI	RAATTY	4 cut(s)	280, 726, 1333, 1520
XbaI	TCTAGA	1 cut(s)	1192
XceI	RCATGY	1 cut(s)	1531
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1370
XhoI	CTCGAG	1 cut(s)	1017
XmaI	CCCGGG	1 cut(s)	745
XmiI	GTMKAC	1 cut(s)	2039
XmnI	GAANNNNTTC	2 cut(s)	41, 1146
XspI	CTAG	2 cut(s)	662, 1193
Zsp2I	ATGCAT	3 cut(s)	135, 1274, 2368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.