Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0001165

Physical Location & Seq

Genomic Coordinates Forward (+)

49797776 .. 49800010

Gene Structure 2235 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG248200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGGCTCTCCAATCTGCTCTGCCATTTGAAATGTACAACCAATTCGCCGATCAATCTTCCAATTCCAATAAGGCCACAAAGAGGGACTTCAGCAATGTCCACGGTTCCGATGATGGCGATGGCGACGCAATCTTGGTCAATGCAATGAAAAGAATCAAGCTCTCATCAGATAATGGTGACTCATCTCAGATGAAGACGAAGAAGAAGGAAATTGGGTATGAAGACGTAGGTGGTGTGAGGAAGCAGATGGCTCAGATTCGTGAGTTAGTGGAGCTTCCATTGAAGCAACCCAAGTTGTTTAAGTTCATTGGAGTGAAGCCTCCAAAGGGTATTCTTCTATACGGCCCCCCTGGCTCGGGCAAGACTCTGATTGCCCGAGCCCTCGCGAACGAGACGGGGGCCTTGTTTTTGTGCATTAACGGGCCGGAGATCATGTCGAAAATGGCTGGGGAGAGTGAGAGCAATCTAAGGAAGGTTTTCGAGGAAGCGGAGAAGAATGCACCGTCTATTGTGTTTATAGATGAGATTGACTCAATTGCTCCGAAGAGAGAGAAGAGTAATGGGGAAGTTGAGAGGAGGATTGTTTCACAGCTCTTGACACTCATGGATGGTATGAAGTCGAAAGGGCGTGTGATTGTTATTGGGGCCACGAATCGGCCTAATAGTATTGACCCGGCTCTTAGGAGGTGTGGAAGATTTGATAGGGAGATAGATATTGGTGTTCCTGATGAGGCCGGACGGCTTGAGGTGCTTCGAATCCATACCAAGAACATGAAGCTTGGTGAAGATGTGGAGTTGGAGAAGATAGCCAAGGGGACACATGGGTATGTTGGGGCAGATTTAGCTGCATTGTGCACTGAGGCTGCGCTTCAATGCATAAGAGAGAATTTGGATGAAATTGACTGGGAAGATCAGGAGACATTAGATGCTGCGATGCTTGGCTCTATGAAAGTTGGAAATGAGCACTTTCGGATTGCTTTGCGAGCAAGCAACCCTTCTGCTCTTCGCGAAATGGCTGTGGAGGTGCCGAATGTGACTTGGGATGCTATCGGGGGTCTGGAGAAAGTGAAGAGGGAACTTCAGGAGACTGTTCAGTATCCAGTTGAGCGTCCTGAGAAGTTTGAGAAATATGGGATGTCACCCTCAAAGGGGGTTCTTTTCTATGGCCCTCCTGGTTGTGGTAAGACCCTTTTGGCCAAGGCTATTGCCAATGAGTGTCAGGCAAATTTTATTAGTATCAAGGGGCCTGAGTTGCTTACTAGTGGGTTTGGAGAGAGTGAGGCGAATGTGAGGGAAATTTTCGACAAGGCTAGAGGGTCTGCTCCCTGTGTCCTCTTTTTTGATGAACTCGATTCCATTGCTACTCAGAGAAGTAGTAATGCTGGAGGTGGTGCTGCTGCTGATAGGGTTTTGAACCAACTCCTTACAGAAATGGATGGATTGTCTGCTAAGAAAACTGTCTTTGTTATCGGGGCCACCAATAGACCTGACATTATAGACCCTGCACTTCTGCGTCCAGGACGTCTGGACCAATTGATTTACATCCCTCTCCCTGATGAGGAATCCCGCTGTGAAATATTCAAGGCTTGCTTGAGGAAGTCTCCAGTTTCCAAAGATGTGAACATCAGAGCTCTTGCAAAGAACACTAGAGGATTTAGTGGTGCTGATATCACAGAAATATGCCAGCGTGCTTGCAAATATGCCATTAGAGAGAACATTGGGAATGATATCAAGAGGGAGATGAGCAGAAGTGAGAATTGTGAAGCAATGGAGGAGGATGAAGTGGCAAAGATCACGGCAGCTCACTTCGTGGAGTCGATGAAGTATGCTCGTAGAAGTGTTGGTGATTCTGATATCCGCAAATACAAGGCATACGCTCAGACATTGCAGCAGTCTAGAGGGTTTGGAACAGACCAGTTCAAAATTGCAGGGAGCGGTTCTCATGCTGCTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010038 GO:0010604 GO:0012505 GO:0016020 GO:0019222 GO:0031974 GO:0031981 GO:0042176 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046686 GO:0048518 GO:0050789 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0070013 GO:0071944 GO:0080090

KEGG Pathways

Metabolic & Signaling

ko04141 ko05134 map04141 map05134

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

651

Amino Acids

71.42

Weight (kDa)

6.25

Isoelectric Point (pI)

45.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RuvB_N	PF05496	110 - 184	5.5e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA	PF00004	111 - 240	1.2e-48	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	263 - 306	1.2e-16	AAA+ lid domain
RuvB_N	PF05496	382 - 421	7.7e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA	PF00004	385 - 517	4e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	539 - 575	1.1e-11	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53230
fragaria_vesca	FvH4_2g06940 FvH4_2g06950 FvH4_2g07260 FvH4_4g17680 FvH4_6g16220 FvH4_6g16220
malus_domestica	MD03G1125700.v1.1 MD04G1094400.v1.1 MD11G1142500.v1.1 MD12G1114300.v1.1 MD17G1225300.v1.1 MD17G1225400.v1.1 MD17G1225600.v1.1 MD17G1228800.v1.1
prunus_persica	Prupe.4G237600_v2.0.a1 Prupe.6G107800_v2.0.a1 Prupe.6G233700_v2.0.a1 Prupe.6G233700_v2.0.a1
pyrus_communis	pycom04g08940 pycom04g08950 pycom09g14580 pycom11g11820 pycom12g11360
rosa_chinensis	RchiOBHm_Chr3g0470061 RchiOBHm_Chr4g0421621 RchiOBHm_Chr6g0259281 RchiOBHm_Chr6g0259871
rosa_laevigata	RLG00000007663 RLG00000014503 RLG00000014543 RLG00000024300
rosa_multiflora	Rmu_co8522155.1_g000003 Rmu_sc0005600.1_g000001 Rmu_sc0009049.1_g000005 Rmu_sc0011974.1_g000005 Rmu_sc0033604.1_g000001 Rmu_sc0033605.1_g000001 Rmu_ssc0000050.1_g000089
rosa_roxburghii	Rroxscaffold_5G00363870 Rroxscaffold_6G00411220
rosa_rugosa	Rorug03G0106600 Rorug04G0173900 Rorug05G0586800 Rorug05G0590900
rosa_samantha	Rh3AG155800 Rh4CG248200 Rh6AG103300 Rh6AG103400 Rh6AG107600 Rh6BG095700 Rh6CG092600 Rh6CG096400
rosa_wichuraiana	Rw3G014670 Rw4G020040 Rw6G008920 Rw6G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1525
AccB1I	GGYRCC	1 cut(s)	1024
AccBSI	CCGCTC	1 cut(s)	1935
AccII	CGCG	2 cut(s)	386, 1008
AciI	CCGC	4 cut(s)	488, 1567, 1858, 1935
AcoI	YGGCCR	1 cut(s)	1194
AcsI	RAATTY	3 cut(s)	886, 1225, 1296
AcuI	CTGAAG	2 cut(s)	73, 1064
AcyI	GRCGYC	1 cut(s)	1522
AdeI	CACNNNGTG	1 cut(s)	1810
AfaI	GTAC	1 cut(s)	35
AfiI	CCNNNNNNNGG	9 cut(s)	81, 326, 355, 356, 654, 1148, 1149, 1178, 1558
AgsI	TTSAA	6 cut(s)	29, 283, 872, 1414, 1582, 1921
AhlI	ACTAGT	1 cut(s)	1259
AjnI	CCWGG	3 cut(s)	349, 1171, 1516
AluBI	AGCT	7 cut(s)	160, 274, 592, 778, 845, 1631, 1802
AluI	AGCT	7 cut(s)	160, 274, 592, 778, 845, 1631, 1802
Alw21I	GWGCWC	3 cut(s)	857, 966, 1633
Alw26I	GTCTC	4 cut(s)	386, 911, 1079, 1605
Alw44I	GTGCAC	1 cut(s)	853
Ama87I	CYCGRG	2 cut(s)	355, 375
ApaLI	GTGCAC	1 cut(s)	853
ApeKI	GCWGC	8 cut(s)	845, 863, 929, 1394, 1397, 1799, 1888, 1946
ApoI	RAATTY	3 cut(s)	886, 1225, 1296
Asp700I	GAANNNNTTC	1 cut(s)	476
AspLEI	GCGC	1 cut(s)	868
AspS9I	GGNCC	8 cut(s)	344, 399, 422, 645, 1166, 1244, 1473, 1528
AsuC2I	CCSGG	1 cut(s)	674
AsuHPI	GGTGA	4 cut(s)	188, 794, 1131, 1856
AsuII	TTCGAA	1 cut(s)	754
AvaI	CYCGRG	2 cut(s)	355, 375
AvaII	GGWCC	1 cut(s)	1528
BaeGI	GKGCMC	1 cut(s)	857
BalI	TGGCCA	1 cut(s)	1196
BanI	GGYRCC	1 cut(s)	1024
BanII	GRGCYC	2 cut(s)	382, 1633
BbsI	GAAGAC	2 cut(s)	200, 228
Bbv12I	GWGCWC	3 cut(s)	857, 966, 1633
BbvI	GCAGC	8 cut(s)	832, 850, 916, 1381, 1384, 1811, 1900, 1933
BccI	CCATC	5 cut(s)	107, 113, 241, 602, 1430
BceAI	ACGGC	3 cut(s)	358, 755, 1812
BcgI	CGANNNNNNTGC	2 cut(s)	1340, 1374
BciT130I	CCWGG	3 cut(s)	351, 1173, 1518
BciVI	GTATCC	1 cut(s)	1107
BcnI	CCSGG	1 cut(s)	674
BcoDI	GTCTC	4 cut(s)	386, 911, 1079, 1605
BcuI	ACTAGT	1 cut(s)	1259
BfaI	CTAG	4 cut(s)	1260, 1311, 1647, 1896
BfuI	GTATCC	1 cut(s)	1107
BglI	GCCNNNNNGGC	1 cut(s)	351
BisI	GCNGC	8 cut(s)	846, 864, 930, 1395, 1398, 1800, 1889, 1947
BlsI	GCNGC	8 cut(s)	847, 865, 931, 1396, 1399, 1801, 1890, 1948
Bme1390I	CCNGG	4 cut(s)	351, 674, 1173, 1518
Bme18I	GGWCC	1 cut(s)	1528
BmeT110I	CYCGRG	2 cut(s)	355, 375
BmgT120I	GGNCC	8 cut(s)	344, 399, 422, 645, 1166, 1244, 1473, 1528
BmiI	GGNNCC	7 cut(s)	106, 346, 400, 646, 1026, 1245, 1474
BmrFI	CCNGG	4 cut(s)	351, 674, 1173, 1518
BmrI	ACTGGG	1 cut(s)	913
BmsI	GCATC	3 cut(s)	916, 924, 1033
BmuI	ACTGGG	1 cut(s)	913
BpiI	GAAGAC	2 cut(s)	200, 228
BplI	GAGNNNNNCTC	6 cut(s)	515, 547, 1585, 1617, 1924, 1956
BpmI	CTGGAG	3 cut(s)	1079, 1404, 1587
Bpu14I	TTCGAA	1 cut(s)	754
BpuEI	CTTGAG	2 cut(s)	764, 1612
BpuMI	CCSGG	1 cut(s)	674
BsaBI	GATNNNNATC	1 cut(s)	1541
BsaHI	GRCGYC	1 cut(s)	1522
BsaJI	CCNNGG	4 cut(s)	100, 349, 810, 1197
BsaXI	ACNNNNNCTCC	2 cut(s)	419, 449
Bsc4I	CCNNNNNNNGG	9 cut(s)	81, 326, 355, 356, 654, 1148, 1149, 1178, 1558
Bse1I	ACTGG	4 cut(s)	908, 1100, 1604, 1915
Bse3DI	GCAATG	5 cut(s)	100, 150, 1356, 1773, 1883
Bse8I	GATNNNNATC	1 cut(s)	1541
BseBI	CCWGG	3 cut(s)	351, 1173, 1518
BseDI	CCNNGG	4 cut(s)	100, 349, 810, 1197
BseGI	GGATG	7 cut(s)	613, 898, 1048, 1140, 1441, 1542, 1783
BseJI	GATNNNNATC	1 cut(s)	1541
BseLI	CCNNNNNNNGG	9 cut(s)	81, 326, 355, 356, 654, 1148, 1149, 1178, 1558
BseMI	GCAATG	5 cut(s)	100, 150, 1356, 1773, 1883
BseMII	CTCAG	7 cut(s)	200, 266, 849, 1104, 1239, 1379, 1892
BseNI	ACTGG	4 cut(s)	908, 1100, 1604, 1915
BseRI	GAGGAG	2 cut(s)	589, 1787
BseSI	GKGCMC	1 cut(s)	857
BseXI	GCAGC	8 cut(s)	832, 850, 916, 1381, 1384, 1811, 1900, 1933
BseYI	CCCAGC	1 cut(s)	446
BsgI	GTGCAG	1 cut(s)	1488
Bsh1236I	CGCG	2 cut(s)	386, 1008
BshNI	GGYRCC	1 cut(s)	1024
BsiHKAI	GWGCWC	3 cut(s)	857, 966, 1633
BsiHKCI	CYCGRG	2 cut(s)	355, 375
BsiSI	CCGG	3 cut(s)	425, 674, 735
BslFI	GGGAC	2 cut(s)	98, 829
BslI	CCNNNNNNNGG	9 cut(s)	81, 326, 355, 356, 654, 1148, 1149, 1178, 1558
BsmAI	GTCTC	4 cut(s)	386, 911, 1079, 1605
BsmBI	CGTCTC	1 cut(s)	386
BsmFI	GGGAC	2 cut(s)	98, 829
BsmI	GAATGC	1 cut(s)	502
BsoBI	CYCGRG	2 cut(s)	355, 375
Bsp119I	TTCGAA	1 cut(s)	754
Bsp1286I	GDGCHC	4 cut(s)	382, 857, 966, 1633
Bsp1407I	TGTACA	1 cut(s)	33
Bsp143I	GATC	4 cut(s)	49, 429, 910, 1791
Bsp68I	TCGCGA	2 cut(s)	386, 1008
BspACI	CCGC	4 cut(s)	488, 1567, 1858, 1935
BspCNI	CTCAG	7 cut(s)	199, 265, 850, 1105, 1240, 1378, 1891
BspFNI	CGCG	2 cut(s)	386, 1008
BspLI	GGNNCC	7 cut(s)	106, 346, 400, 646, 1026, 1245, 1474
BspQI	GCTCTTC	1 cut(s)	1008
BspT104I	TTCGAA	1 cut(s)	754
BspT107I	GGYRCC	1 cut(s)	1024
BsrBI	CCGCTC	1 cut(s)	1935
BsrDI	GCAATG	5 cut(s)	100, 150, 1356, 1773, 1883
BsrGI	TGTACA	1 cut(s)	33
BsrI	ACTGG	4 cut(s)	908, 1100, 1604, 1915
BssECI	CCNNGG	4 cut(s)	100, 349, 810, 1197
BssMI	GATC	4 cut(s)	49, 429, 910, 1791
BssNI	GRCGYC	1 cut(s)	1522
BssT1I	CCWWGG	2 cut(s)	810, 1197
Bst2UI	CCWGG	3 cut(s)	351, 1173, 1518
Bst4CI	ACNGT	4 cut(s)	104, 504, 1090, 1459
Bst6I	CTCTTC	4 cut(s)	539, 548, 1008, 1064
BstACI	GRCGYC	1 cut(s)	1522
BstAUI	TGTACA	1 cut(s)	33
BstBI	TTCGAA	1 cut(s)	754
BstC8I	GCNNGC	6 cut(s)	984, 988, 1588, 1685, 1689, 1693
BstDSI	CCRYGG	1 cut(s)	100
BstF5I	GGATG	7 cut(s)	613, 898, 1048, 1140, 1441, 1542, 1783
BstFNI	CGCG	2 cut(s)	386, 1008
BstHHI	GCGC	1 cut(s)	868
BstKTI	GATC	4 cut(s)	52, 432, 913, 1794
BstMAI	GTCTC	4 cut(s)	386, 911, 1079, 1605
BstMBI	GATC	4 cut(s)	49, 429, 910, 1791
BstMWI	GCNNNNNNNGC	5 cut(s)	351, 748, 842, 983, 1252
BstNI	CCWGG	3 cut(s)	351, 1173, 1518
BstSCI	CCNGG	4 cut(s)	349, 672, 1171, 1516
BstSLI	GKGCMC	1 cut(s)	857
BstUI	CGCG	2 cut(s)	386, 1008
BstV1I	GCAGC	8 cut(s)	832, 850, 916, 1381, 1384, 1811, 1900, 1933
BstV2I	GAAGAC	2 cut(s)	200, 228
BsuI	GTATCC	1 cut(s)	1107
BtgI	CCRYGG	1 cut(s)	100
BtgZI	GCGATG	2 cut(s)	132, 947
BtsCI	GGATG	7 cut(s)	613, 898, 1048, 1140, 1441, 1542, 1783
BtsIMutI	CAGTG	1 cut(s)	855
BtuMI	TCGCGA	2 cut(s)	386, 1008
Cac8I	GCNNGC	6 cut(s)	984, 988, 1588, 1685, 1689, 1693
CfoI	GCGC	1 cut(s)	868
Cfr13I	GGNCC	8 cut(s)	344, 399, 422, 645, 1166, 1244, 1473, 1528
CseI	GACGC	3 cut(s)	134, 1097, 1502
Csp6I	GTAC	1 cut(s)	34
CviAII	CATG	5 cut(s)	433, 604, 772, 821, 1943
CviQI	GTAC	1 cut(s)	34
DpnI	GATC	4 cut(s)	51, 431, 912, 1793
DpnII	GATC	4 cut(s)	49, 429, 910, 1791
DraIII	CACNNNGTG	1 cut(s)	1810
EaeI	YGGCCR	1 cut(s)	1194
Eam1104I	CTCTTC	4 cut(s)	539, 548, 1008, 1064
EarI	CTCTTC	4 cut(s)	539, 548, 1008, 1064
Ecl136II	GAGCTC	1 cut(s)	1631
Eco130I	CCWWGG	2 cut(s)	810, 1197
Eco24I	GRGCYC	2 cut(s)	382, 1633
Eco32I	GATATC	3 cut(s)	1669, 1729, 1855
Eco47I	GGWCC	1 cut(s)	1528
Eco53kI	GAGCTC	1 cut(s)	1631
Eco57I	CTGAAG	2 cut(s)	73, 1064
Eco88I	CYCGRG	2 cut(s)	355, 375
EcoICRI	GAGCTC	1 cut(s)	1631
EcoO109I	RGGNCCY	2 cut(s)	399, 1244
EcoRII	CCWGG	3 cut(s)	349, 1171, 1516
EcoRV	GATATC	3 cut(s)	1669, 1729, 1855
EcoT14I	CCWWGG	2 cut(s)	810, 1197
EcoT22I	ATGCAT	1 cut(s)	878
EcoT38I	GRGCYC	2 cut(s)	382, 1633
ErhI	CCWWGG	2 cut(s)	810, 1197
Esp3I	CGTCTC	1 cut(s)	386
FaeI	CATG	5 cut(s)	436, 607, 775, 824, 1946
FalI	AAGNNNNNCTT	6 cut(s)	71, 103, 979, 1011, 1574, 1606
FaqI	GGGAC	2 cut(s)	98, 829
FatI	CATG	5 cut(s)	432, 603, 771, 820, 1942
FauI	CCCGC	1 cut(s)	1574
Fnu4HI	GCNGC	8 cut(s)	846, 864, 930, 1395, 1398, 1800, 1889, 1947
FokI	GGATG	7 cut(s)	620, 905, 1055, 1147, 1448, 1529, 1790
FriOI	GRGCYC	2 cut(s)	382, 1633
Fsp4HI	GCNGC	8 cut(s)	846, 864, 930, 1395, 1398, 1800, 1889, 1947
FspBI	CTAG	4 cut(s)	1260, 1311, 1647, 1896
GlaI	GCGC	1 cut(s)	867
GluI	GCNGC	8 cut(s)	846, 864, 930, 1395, 1398, 1800, 1889, 1947
GsaI	CCCAGC	1 cut(s)	450
GsuI	CTGGAG	3 cut(s)	1079, 1404, 1587
HapII	CCGG	3 cut(s)	425, 674, 735
HgaI	GACGC	3 cut(s)	134, 1097, 1502
HhaI	GCGC	1 cut(s)	868
Hin1I	GRCGYC	1 cut(s)	1522
Hin1II	CATG	5 cut(s)	436, 607, 775, 824, 1946
Hin6I	GCGC	1 cut(s)	866
HinP1I	GCGC	1 cut(s)	866
HindIII	AAGCTT	1 cut(s)	776
HpaII	CCGG	3 cut(s)	425, 674, 735
HphI	GGTGA	4 cut(s)	188, 794, 1131, 1856
Hpy166II	GTNNAC	3 cut(s)	100, 855, 1621
Hpy8I	GTNNAC	3 cut(s)	100, 855, 1621
Hpy99I	CGWCG	1 cut(s)	128
HpyAV	CCTTC	3 cut(s)	199, 466, 1005
HpyCH4III	ACNGT	4 cut(s)	104, 504, 1090, 1459
HpyCH4IV	ACGT	2 cut(s)	225, 1522
HpyF10VI	GCNNNNNNNGC	5 cut(s)	351, 748, 842, 983, 1252
HpySE526I	ACGT	2 cut(s)	225, 1522
Hsp92I	GRCGYC	1 cut(s)	1522
Hsp92II	CATG	5 cut(s)	436, 607, 775, 824, 1946
HspAI	GCGC	1 cut(s)	866
Kzo9I	GATC	4 cut(s)	49, 429, 910, 1791
LguI	GCTCTTC	1 cut(s)	1008
LmnI	GCTCC	4 cut(s)	271, 544, 1327, 1932
Lsp1109I	GCAGC	8 cut(s)	832, 850, 916, 1381, 1384, 1811, 1900, 1933
LweI	GCATC	3 cut(s)	916, 924, 1033
MaeI	CTAG	4 cut(s)	1260, 1311, 1647, 1896
MaeII	ACGT	2 cut(s)	225, 1522
MaeIII	GTNAC	3 cut(s)	176, 1033, 1137
MalI	GATC	4 cut(s)	51, 431, 912, 1793
MbiI	CCGCTC	1 cut(s)	1935
MboI	GATC	4 cut(s)	49, 429, 910, 1791
MfeI	CAATTG	2 cut(s)	534, 1532
MhlI	GDGCHC	4 cut(s)	382, 857, 966, 1633
MlsI	TGGCCA	1 cut(s)	1196
MluNI	TGGCCA	1 cut(s)	1196
MlyI	GAGTC	4 cut(s)	173, 358, 524, 1823
MmeI	TCCRAC	2 cut(s)	777, 934
Mox20I	TGGCCA	1 cut(s)	1196
Mph1103I	ATGCAT	1 cut(s)	878
MroXI	GAANNNNTTC	1 cut(s)	476
MscI	TGGCCA	1 cut(s)	1196
MseI	TTAA	2 cut(s)	300, 417
MslI	CAYNNNNRTG	2 cut(s)	311, 825
Msp20I	TGGCCA	1 cut(s)	1196
MspA1I	CMGCKG	1 cut(s)	1569
MspI	CCGG	3 cut(s)	425, 674, 735
MspR9I	CCNGG	4 cut(s)	351, 674, 1173, 1518
MunI	CAATTG	2 cut(s)	534, 1532
Mva1269I	GAATGC	1 cut(s)	502
MvaI	CCWGG	3 cut(s)	351, 1173, 1518
MvnI	CGCG	2 cut(s)	386, 1008
MwoI	GCNNNNNNNGC	5 cut(s)	351, 748, 842, 983, 1252
NciI	CCSGG	1 cut(s)	674
NdeII	GATC	4 cut(s)	49, 429, 910, 1791
NlaIII	CATG	5 cut(s)	436, 607, 775, 824, 1946
NlaIV	GGNNCC	7 cut(s)	106, 346, 400, 646, 1026, 1245, 1474
NmuCI	GTSAC	3 cut(s)	176, 1033, 1137
NruI	TCGCGA	2 cut(s)	386, 1008
NsiI	ATGCAT	1 cut(s)	878
NspV	TTCGAA	1 cut(s)	754
PciSI	GCTCTTC	1 cut(s)	1008
PctI	GAATGC	1 cut(s)	502
PdmI	GAANNNNTTC	1 cut(s)	476
PfeI	GAWTC	7 cut(s)	153, 256, 652, 756, 1352, 1562, 1847
PfoI	TCCNGGA	1 cut(s)	1516
PkrI	GCNGC	8 cut(s)	847, 865, 931, 1396, 1399, 1801, 1890, 1948
PleI	GAGTC	4 cut(s)	173, 358, 524, 1822
PpsI	GAGTC	4 cut(s)	173, 358, 524, 1822
Psp124BI	GAGCTC	1 cut(s)	1633
Psp6I	CCWGG	3 cut(s)	349, 1171, 1516
PspFI	CCCAGC	1 cut(s)	446
PspGI	CCWGG	3 cut(s)	349, 1171, 1516
PspN4I	GGNNCC	7 cut(s)	106, 346, 400, 646, 1026, 1245, 1474
PspPI	GGNCC	8 cut(s)	344, 399, 422, 645, 1166, 1244, 1473, 1528
RruI	TCGCGA	2 cut(s)	386, 1008
RsaI	GTAC	1 cut(s)	35
RsaNI	GTAC	1 cut(s)	34
RseI	CAYNNNNRTG	2 cut(s)	311, 825
SacI	GAGCTC	1 cut(s)	1633
SapI	GCTCTTC	1 cut(s)	1008
SaqAI	TTAA	2 cut(s)	300, 417
SatI	GCNGC	8 cut(s)	846, 864, 930, 1395, 1398, 1800, 1889, 1947
Sau3AI	GATC	4 cut(s)	49, 429, 910, 1791
Sau96I	GGNCC	8 cut(s)	344, 399, 422, 645, 1166, 1244, 1473, 1528
SchI	GAGTC	4 cut(s)	173, 358, 524, 1823
ScrFI	CCNGG	4 cut(s)	351, 674, 1173, 1518
SduI	GDGCHC	4 cut(s)	382, 857, 966, 1633
SfaNI	GCATC	3 cut(s)	916, 924, 1033
SfuI	TTCGAA	1 cut(s)	754
SinI	GGWCC	1 cut(s)	1528
SmiMI	CAYNNNNRTG	2 cut(s)	311, 825
SmlI	CTYRAG	2 cut(s)	743, 1591
SmoI	CTYRAG	2 cut(s)	743, 1591
SpeI	ACTAGT	1 cut(s)	1259
SsiI	CCGC	4 cut(s)	488, 1567, 1858, 1935
SspI	AATATT	1 cut(s)	1578
SspMI	CTAG	4 cut(s)	1260, 1311, 1647, 1896
SstI	GAGCTC	1 cut(s)	1633
StyD4I	CCNGG	4 cut(s)	349, 672, 1171, 1516
StyI	CCWWGG	2 cut(s)	810, 1197
TaaI	ACNGT	4 cut(s)	104, 504, 1090, 1459
TaiI	ACGT	2 cut(s)	228, 1525
TaqI	TCGA	7 cut(s)	437, 480, 620, 754, 1302, 1350, 1817
TatI	WGTACW	1 cut(s)	33
TfiI	GAWTC	7 cut(s)	153, 256, 652, 756, 1352, 1562, 1847
Tru1I	TTAA	2 cut(s)	300, 417
Tru9I	TTAA	2 cut(s)	300, 417
TscAI	CASTG	1 cut(s)	862
TseFI	GTSAC	3 cut(s)	176, 1033, 1137
TseI	GCWGC	8 cut(s)	845, 863, 929, 1394, 1397, 1799, 1888, 1946
Tsp45I	GTSAC	3 cut(s)	176, 1033, 1137
TspRI	CASTG	1 cut(s)	862
VneI	GTGCAC	1 cut(s)	853
VpaK11BI	GGWCC	1 cut(s)	1528
XapI	RAATTY	3 cut(s)	886, 1225, 1296
XbaI	TCTAGA	1 cut(s)	1895
XmnI	GAANNNNTTC	1 cut(s)	476
XspI	CTAG	4 cut(s)	1260, 1311, 1647, 1896
ZraI	GACGTC	1 cut(s)	1523
Zsp2I	ATGCAT	1 cut(s)	878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.