FvH4_2g07260

Cell Division

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001165
Physical Location & Seq
Forward (+)
6005359 .. 6008089
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07260.t1

Sequence Viewer

Length: 2400 bp
ATGACGAACCAATCTGAAGGAACAAATAAGAGGGACTTTAGCACGGCCATTCTCAACCGCAAGAAGGCTCCAAACCGCCTTATTGTGGATGAAGCTGTGATGGATGATAACTCTGTCGTCCAACTCCACCCGGAGACCATAGAGAAGCTCCAGCTCTTTCATGGCGATACGGTCCTGATCAAGGGAAAGAAAAGGAGAGATACAATCTGCATTGCAACTGCTGATGATTCCTGTGAAGGACCAAAGATAAGGATGAACAAGGTTGTGAGGAACAACCTTAGGGTTAGGCTAGGTGATGTTGTTTCCATTCACCAGTGCGCTGATGTCAAGAATGGAAAGCGTGTCCACATTCTCCCAATAGATGACACCATTGAATGCATGACTGGAAATCTATTTGATGTCTACTTGAGGCCTTATTTCCAAGTGACCTACCGCCCGGTGAGGAAAAGTGACCTTTTCCTAGCCAGAGGAGGAATGAGGAGTGTAGAGTTTAAGGTTGTTGAGACTGACCCGGCTGAGTACTGTGTGGTTGCTCCAGACACCGAAATCTTCTGCGAGGGTGAACCTGTTAGAAGGGAGGATGAGGAGAGATTAGATGAGGTAGGGTATGATGATATCGGTGGTGTTAGGAAACAGATGGCTCAGATTCGCGAATTGGTTGAGCTGCCTCTGAGGCATCCTCAGTTGTTTAAATCGATTGGAGTCAAGCCACCAAAGGGTATTCTTCTCTATGGTCCTCCTGGAACAGGCAAGACTTTAATTGCAAGAGCTGTTGCTAATGAAACCGGGGCGTTCTTTTTATGCATCAATGGACCAGAAATCATGTCCAAGATGGCTGGAGAGAGTGAAAGCAACCTCAGGAAAGCTTTCGAGGAAGCAGAGAAGAATGCGCCATCAATCATATTTATTGATGAGATTGATTCAATTGCTCCCAAGAGAGAGAAGACTAATGGGGAAATTGAAAGGAGGATTGTTTCCCAGCTGTTGACACTTATGGATGGCCTAAAGTCTCGAGCACATGTGATTGTGATGGCAGCTACTAATCGTCCAAACAGCATTGACCCTGCTCTTAGAAGATTTGGAAGATTTGATAGGGAAATAGACATTGGTGTTCCAGATGAAGTCGGGCGTCTTGAGGTCCTCCGCATTCATAGCAAGAACATGAAGCTTGCTGAAGATGTCGATTTGGAAAGGGTTGCCAAGGATACTCATGGCTATGTTGGTGCTGACCTAGCTGCTTTAGCTACTGAGGCTGCACTTCAATGCATCCGAGAGAAGATGGATGTGATTGACTTGGAAGACGAGACAATAGATGCTGAGATACTCAACTCAATGGCAGTGACAAACGAACACTTCAAGACTGCCCTTGGATCAAGCAACCCTTCTGCTCTTCGTGAAACGGTTGTTGAAGTTCCCAATGTCTCTTGGGATGCAATTGGAGGCCTCGAAAAGGTTAAGAGAGAACTTCAAGAGACTGTTCAATATCCTGTTGAGCATCCGGAGAAATTTGAGAAATTCGGTATGTCACCTTCAAAGGGAGTTCTCTTCTATGGCCCTCCTGGATGCGGTAAAACTCTTTTGGCCAAGGCTATTGCTAATGAGTGTCAGGCTAATTTCATCAGCATCAAGGGACCTGAGTTGCTCACCATGTGGTTTGGAGAGAGTGAGGCCAATGTAAGAGAAATTTTCGACAAGGCTCGTGCATCTGCTCCATGTGTCCTCTTCTTCGATGAGCTTGACTCCATTGCTACTAAAAGAGGTAGCAGTGTAGGTGATTCTGGAGGTGCTGATAGGGTTTTGAACCAACTGCTTACAGAAATGGATGGAGTGTCTGCGAAGAAAACAGTCTTCATTATTGGGGCAACCAACAGACCTGACATCATAGACCCTGCACTTCTTCGTCCAGGACGTCTGGATCAGTTGCTTTACATCCCTCTCCCTGATGAGGAATCTCGCCATCAAATATTCAAGGCTTGCTTGAGGAAATCTCCGGTTTCCAAAGATGTGGACCTCAATGTTCTTGCCAAGAACACACAAGGTTTCAGTGGTGCTGATATCACAGAAATATGCCGGCGTGCTTGCAAATATGCCATCAGAGAAAACATTGAGAAAGATATGGAGAGGGAGAGGAGGAGAAGTGAAAACCCTGAGATTATGGAAGATGATGAGGATGAAGTAGCTGAGATCACCAAAGCTCATTTTGAGGAATCAATGAGGTATGCTCGCAGGAGTGTTAGTGATGCTGACATCCGCAAGTACCAGTCTTTTGCAACTACATTGCACCAGGCAAGAGGGTTGGGAGAGGAGTTCAAGTTCCCCACTGGTTCAGGGGCTGCTGTTGGATCTGATCCGTTTGCAACTACTAATGCTGGTGAAGCTAATGATGATCTTTACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N

Protein Analysis

800

Amino Acids

89.0

Weight (kDa)

5.3

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 26 - 107 7.8e-21 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 128 - 191 3.7e-08 Cell division protein 48 (CDC48), domain 2
RuvB_N PF05496 240 - 314 6.9e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 241 - 370 5.3e-48 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 393 - 434 1.5e-14 AAA+ lid domain
AAA PF00004 514 - 646 6.3e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 668 - 704 5.5e-13 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1912
AccI GTMKAC 1 cut(s) 402
AccII CGCG 1 cut(s) 651
AccIII TCCGGA 1 cut(s) 1498
AciI CCGC 6 cut(s) 58, 76, 433, 1144, 1566, 2251
AclWI GGATC 4 cut(s) 1378, 1923, 2342, 2350
AcoI YGGCCR 2 cut(s) 45, 1581
AcsI RAATTY 3 cut(s) 1505, 1514, 1683
AcuI CTGAAG 2 cut(s) 36, 1194
AcyI GRCGYC 2 cut(s) 1129, 1909
AdeI CACNNNGTG 1 cut(s) 1650
AfaI GTAC 2 cut(s) 521, 2258
AfiI CCNNNNNNNGG 9 cut(s) 64, 85, 181, 716, 746, 1450, 1535, 1565, 1945
AflIII ACRYGT 1 cut(s) 1018
AjnI CCWGG 4 cut(s) 739, 1558, 1903, 2283
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
AloI GAACNNNNNNTCC 2 cut(s) 2297, 2329
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 6 cut(s) 128, 497, 1014, 1298, 1426, 1466
AlwI GGATC 4 cut(s) 1378, 1923, 2342, 2350
AlwNI CAGNNNCTG 1 cut(s) 2333
Ama87I CYCGRG 1 cut(s) 1011
Aor13HI TCCGGA 1 cut(s) 1498
AoxI GGCC 7 cut(s) 45, 410, 1000, 1441, 1552, 1581, 1668
ApeKI GCWGC 5 cut(s) 664, 1034, 1235, 1253, 2333
ApoI RAATTY 3 cut(s) 1505, 1514, 1683
Asp700I GAANNNNTTC 1 cut(s) 866
AspLEI GCGC 2 cut(s) 320, 892
AspS9I GGNCC 8 cut(s) 172, 239, 734, 812, 1138, 1553, 1631, 2008
AsuC2I CCSGG 4 cut(s) 131, 437, 512, 787
AsuHPI GGTGA 9 cut(s) 302, 305, 451, 572, 1518, 1636, 1784, 2179, 2384
AvaI CYCGRG 1 cut(s) 1011
AvaII GGWCC 7 cut(s) 172, 239, 734, 812, 1138, 1631, 2008
AxyI CCTNAGG 2 cut(s) 278, 857
BalI TGGCCA 1 cut(s) 1583
BarI GAAGNNNNNNTAC 2 cut(s) 1513, 1545
BauI CACGAG 1 cut(s) 1698
BbsI GAAGAC 3 cut(s) 950, 1305, 1840
Bbv12I GWGCWC 1 cut(s) 1018
BbvI GCAGC 5 cut(s) 651, 1046, 1222, 1240, 2320
BceAI ACGGC 1 cut(s) 60
BciT130I CCWGG 4 cut(s) 741, 1560, 1905, 2285
BciVI GTATCC 1 cut(s) 1198
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 4 cut(s) 131, 437, 512, 787
BcoDI GTCTC 6 cut(s) 128, 497, 1014, 1298, 1426, 1466
BfaI CTAG 3 cut(s) 290, 461, 1232
BfuI GTATCC 1 cut(s) 1198
BglI GCCNNNNNGGC 1 cut(s) 673
BisI GCNGC 5 cut(s) 665, 1035, 1236, 1254, 2334
BlsI GCNGC 5 cut(s) 666, 1036, 1237, 1255, 2335
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 8 cut(s) 131, 437, 512, 741, 787, 1560, 1905, 2285
Bme18I GGWCC 7 cut(s) 172, 239, 734, 812, 1138, 1631, 2008
BmeT110I CYCGRG 1 cut(s) 1011
BmgT120I GGNCC 8 cut(s) 172, 239, 734, 812, 1138, 1553, 1631, 2008
BmiI GGNNCC 2 cut(s) 69, 1632
BmrFI CCNGG 8 cut(s) 131, 437, 512, 741, 787, 1560, 1905, 2285
BpiI GAAGAC 3 cut(s) 950, 1305, 1840
BplI GAGNNNNNCTC 8 cut(s) 664, 696, 1724, 1756, 1972, 2004, 2206, 2238
BpmI CTGGAG 4 cut(s) 134, 519, 858, 1800
BpuEI CTTGAG 3 cut(s) 427, 1154, 1999
BpuMI CCSGG 4 cut(s) 131, 437, 512, 787
Bsa29I ATCGAT 1 cut(s) 695
BsaHI GRCGYC 2 cut(s) 1129, 1909
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 786, 1200, 1366, 1584
BsaWI WCCGGW 2 cut(s) 1498, 1990
BsaXI ACNNNNNCTCC 6 cut(s) 2122, 2152, 2221, 2251, 2297, 2327
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 85, 181, 716, 746, 1450, 1535, 1565, 1945
Bse118I RCCGGY 1 cut(s) 2070
Bse1I ACTGG 4 cut(s) 313, 388, 2260, 2326
Bse21I CCTNAGG 2 cut(s) 278, 857
Bse3DI GCAATG 3 cut(s) 210, 1743, 2276
BseAI TCCGGA 1 cut(s) 1498
BseBI CCWGG 4 cut(s) 741, 1560, 1905, 2285
BseCI ATCGAT 1 cut(s) 695
BseDI CCNNGG 4 cut(s) 786, 1200, 1366, 1584
BseLI CCNNNNNNNGG 9 cut(s) 64, 85, 181, 716, 746, 1450, 1535, 1565, 1945
BseMI GCAATG 3 cut(s) 210, 1743, 2276
BseNI ACTGG 4 cut(s) 313, 388, 2260, 2326
BseRI GAGGAG 6 cut(s) 483, 493, 599, 2143, 2146, 2318
BseXI GCAGC 5 cut(s) 651, 1046, 1222, 1240, 2320
BseYI CCCAGC 1 cut(s) 978
BsgI GTGCAG 2 cut(s) 1239, 1875
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 7 cut(s) 47, 412, 1002, 1443, 1554, 1583, 1670
BshVI ATCGAT 1 cut(s) 695
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 1011
BsiSI CCGG 7 cut(s) 131, 437, 512, 786, 1499, 1991, 2071
BslFI GGGAC 2 cut(s) 47, 1644
BslI CCNNNNNNNGG 9 cut(s) 64, 85, 181, 716, 746, 1450, 1535, 1565, 1945
BsmAI GTCTC 6 cut(s) 128, 497, 1014, 1298, 1426, 1466
BsmFI GGGAC 2 cut(s) 47, 1644
BsmI GAATGC 3 cut(s) 380, 892, 1146
BsnI GGCC 7 cut(s) 47, 412, 1002, 1443, 1554, 1583, 1670
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 1011
Bsp1286I GDGCHC 1 cut(s) 1018
Bsp13I TCCGGA 1 cut(s) 1498
Bsp143I GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
Bsp68I TCGCGA 1 cut(s) 651
BspACI CCGC 6 cut(s) 58, 76, 433, 1144, 1566, 2251
BspANI GGCC 7 cut(s) 47, 412, 1002, 1443, 1554, 1583, 1670
BspDI ATCGAT 1 cut(s) 695
BspEI TCCGGA 1 cut(s) 1498
BspFNI CGCG 1 cut(s) 651
BspLI GGNNCC 2 cut(s) 69, 1632
BspPI GGATC 4 cut(s) 1378, 1923, 2342, 2350
BspQI GCTCTTC 1 cut(s) 1395
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 3 cut(s) 210, 1743, 2276
BsrFI RCCGGY 1 cut(s) 2070
BsrI ACTGG 4 cut(s) 313, 388, 2260, 2326
BssAI RCCGGY 1 cut(s) 2070
BssECI CCNNGG 4 cut(s) 786, 1200, 1366, 1584
BssMI GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
BssNI GRCGYC 2 cut(s) 1129, 1909
BssSI CACGAG 1 cut(s) 1698
BssT1I CCWWGG 3 cut(s) 1200, 1366, 1584
Bst2BI CACGAG 1 cut(s) 1698
Bst2UI CCWGG 4 cut(s) 741, 1560, 1905, 2285
Bst4CI ACNGT 5 cut(s) 172, 524, 1402, 1477, 1846
Bst6I CTCTTC 3 cut(s) 1395, 1550, 1727
BstACI GRCGYC 2 cut(s) 1129, 1909
BstC8I GCNNGC 6 cut(s) 1170, 1975, 2072, 2076, 2080, 2224
BstENI CCTNNNNNAGG 3 cut(s) 179, 744, 1448
BstFNI CGCG 1 cut(s) 651
BstHHI GCGC 2 cut(s) 320, 892
BstKTI GATC 7 cut(s) 180, 1373, 1918, 2187, 2345, 2350, 2389
BstMAI GTCTC 6 cut(s) 128, 497, 1014, 1298, 1426, 1466
BstMBI GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
BstMWI GCNNNNNNNGC 6 cut(s) 673, 1152, 1232, 1241, 1250, 2375
BstNI CCWGG 4 cut(s) 741, 1560, 1905, 2285
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 8 cut(s) 129, 435, 510, 739, 785, 1558, 1903, 2283
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 5 cut(s) 651, 1046, 1222, 1240, 2320
BstV2I GAAGAC 3 cut(s) 950, 1305, 1840
BstX2I RGATCY 1 cut(s) 2342
BstXI CCANNNNNNTGG 1 cut(s) 2005
BstYI RGATCY 1 cut(s) 2342
Bsu15I ATCGAT 1 cut(s) 695
Bsu36I CCTNAGG 2 cut(s) 278, 857
BsuI GTATCC 1 cut(s) 1198
BsuRI GGCC 7 cut(s) 47, 412, 1002, 1443, 1554, 1583, 1670
BsuTUI ATCGAT 1 cut(s) 695
BtsI GCAGTG 2 cut(s) 1344, 1771
BtsIMutI CAGTG 5 cut(s) 320, 1344, 1771, 2050, 2319
BtuMI TCGCGA 1 cut(s) 651
Cac8I GCNNGC 6 cut(s) 1170, 1975, 2072, 2076, 2080, 2224
CaiI CAGNNNCTG 1 cut(s) 2333
CfoI GCGC 2 cut(s) 320, 892
Cfr10I RCCGGY 1 cut(s) 2070
Cfr13I GGNCC 8 cut(s) 172, 239, 734, 812, 1138, 1553, 1631, 2008
ClaI ATCGAT 1 cut(s) 695
CseI GACGC 1 cut(s) 1118
Csp6I GTAC 2 cut(s) 520, 2257
CviAII CATG 8 cut(s) 161, 379, 823, 1019, 1162, 1211, 1648, 1713
CviQI GTAC 2 cut(s) 520, 2257
DpnI GATC 7 cut(s) 179, 1372, 1917, 2186, 2344, 2349, 2388
DpnII GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
DraI TTTAAA 1 cut(s) 691
DraIII CACNNNGTG 1 cut(s) 1650
EaeI YGGCCR 2 cut(s) 45, 1581
Eam1104I CTCTTC 3 cut(s) 1395, 1550, 1727
EarI CTCTTC 3 cut(s) 1395, 1550, 1727
Eco130I CCWWGG 3 cut(s) 1200, 1366, 1584
Eco147I AGGCCT 2 cut(s) 412, 1443
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 2 cut(s) 616, 2056
Eco47I GGWCC 7 cut(s) 172, 239, 734, 812, 1138, 1631, 2008
Eco57I CTGAAG 2 cut(s) 36, 1194
Eco81I CCTNAGG 2 cut(s) 278, 857
Eco88I CYCGRG 1 cut(s) 1011
EcoNI CCTNNNNNAGG 3 cut(s) 179, 744, 1448
EcoO109I RGGNCCY 2 cut(s) 1138, 1631
EcoRII CCWGG 4 cut(s) 739, 1558, 1903, 2283
EcoRV GATATC 2 cut(s) 616, 2056
EcoT14I CCWWGG 3 cut(s) 1200, 1366, 1584
EcoT22I ATGCAT 3 cut(s) 380, 806, 1268
ErhI CCWWGG 3 cut(s) 1200, 1366, 1584
FaeI CATG 8 cut(s) 164, 382, 826, 1022, 1165, 1214, 1651, 1716
FalI AAGNNNNNCTT 6 cut(s) 20, 52, 1366, 1398, 1961, 1993
FaqI GGGAC 2 cut(s) 47, 1644
FatI CATG 8 cut(s) 160, 378, 822, 1018, 1161, 1210, 1647, 1712
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 5 cut(s) 665, 1035, 1236, 1254, 2334
Fsp4HI GCNGC 5 cut(s) 665, 1035, 1236, 1254, 2334
FspBI CTAG 3 cut(s) 290, 461, 1232
GlaI GCGC 2 cut(s) 319, 891
GluI GCNGC 5 cut(s) 665, 1035, 1236, 1254, 2334
GsaI CCCAGC 1 cut(s) 982
GsuI CTGGAG 4 cut(s) 134, 519, 858, 1800
HaeIII GGCC 7 cut(s) 47, 412, 1002, 1443, 1554, 1583, 1670
HapII CCGG 7 cut(s) 131, 437, 512, 786, 1499, 1991, 2071
HgaI GACGC 1 cut(s) 1118
HhaI GCGC 2 cut(s) 320, 892
Hin1I GRCGYC 2 cut(s) 1129, 1909
Hin1II CATG 8 cut(s) 164, 382, 826, 1022, 1165, 1214, 1651, 1716
Hin6I GCGC 2 cut(s) 318, 890
HinP1I GCGC 2 cut(s) 318, 890
HincII GTYRAC 1 cut(s) 987
HindII GTYRAC 1 cut(s) 987
HindIII AAGCTT 2 cut(s) 864, 1166
HinfI GANTC 8 cut(s) 227, 646, 702, 920, 1739, 1775, 1949, 2207
HpaII CCGG 7 cut(s) 131, 437, 512, 786, 1499, 1991, 2071
HphI GGTGA 9 cut(s) 302, 305, 451, 572, 1518, 1636, 1784, 2179, 2384
Hpy166II GTNNAC 5 cut(s) 346, 403, 563, 987, 2008
Hpy188I TCNGA 6 cut(s) 16, 645, 672, 1271, 2096, 2347
Hpy8I GTNNAC 5 cut(s) 346, 403, 563, 987, 2008
HpyAV CCTTC 6 cut(s) 11, 58, 230, 567, 1392, 1539
HpyCH4III ACNGT 5 cut(s) 172, 524, 1402, 1477, 1846
HpyCH4IV ACGT 1 cut(s) 1909
HpyF10VI GCNNNNNNNGC 6 cut(s) 673, 1152, 1232, 1241, 1250, 2375
HpySE526I ACGT 1 cut(s) 1909
Hsp92I GRCGYC 2 cut(s) 1129, 1909
Hsp92II CATG 8 cut(s) 164, 382, 826, 1022, 1165, 1214, 1651, 1716
HspAI GCGC 2 cut(s) 318, 890
Kpn2I TCCGGA 1 cut(s) 1498
KroI GCCGGC 1 cut(s) 2070
KroNI GCCGGC 1 cut(s) 2072
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
LguI GCTCTTC 1 cut(s) 1395
LmnI GCTCC 5 cut(s) 73, 153, 538, 934, 1714
Lsp1109I GCAGC 5 cut(s) 651, 1046, 1222, 1240, 2320
MaeI CTAG 3 cut(s) 290, 461, 1232
MaeII ACGT 1 cut(s) 1909
MaeIII GTNAC 4 cut(s) 424, 449, 1339, 1524
MalI GATC 7 cut(s) 179, 1372, 1917, 2186, 2344, 2349, 2388
MboI GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
MfeI CAATTG 2 cut(s) 924, 1434
MflI RGATCY 1 cut(s) 2342
MhlI GDGCHC 1 cut(s) 1018
MlsI TGGCCA 1 cut(s) 1583
MluCI AATT 9 cut(s) 653, 759, 924, 957, 1434, 1505, 1514, 1612, 1683
MluNI TGGCCA 1 cut(s) 1583
MlyI GAGTC 2 cut(s) 711, 1733
MmeI TCCRAC 2 cut(s) 145, 2320
Mox20I TGGCCA 1 cut(s) 1583
Mph1103I ATGCAT 3 cut(s) 380, 806, 1268
MroI TCCGGA 1 cut(s) 1498
MroNI GCCGGC 1 cut(s) 2070
MroXI GAANNNNTTC 1 cut(s) 866
MscI TGGCCA 1 cut(s) 1583
MseI TTAA 4 cut(s) 492, 690, 758, 1455
MslI CAYNNNNRTG 2 cut(s) 1215, 1261
Msp20I TGGCCA 1 cut(s) 1583
MspA1I CMGCKG 1 cut(s) 982
MspI CCGG 7 cut(s) 131, 437, 512, 786, 1499, 1991, 2071
MspR9I CCNGG 8 cut(s) 131, 437, 512, 741, 787, 1560, 1905, 2285
MunI CAATTG 2 cut(s) 924, 1434
Mva1269I GAATGC 3 cut(s) 380, 892, 1146
MvaI CCWGG 4 cut(s) 741, 1560, 1905, 2285
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 6 cut(s) 673, 1152, 1232, 1241, 1250, 2375
NaeI GCCGGC 1 cut(s) 2072
NciI CCSGG 4 cut(s) 131, 437, 512, 787
NdeII GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
NgoMIV GCCGGC 1 cut(s) 2070
NlaIII CATG 8 cut(s) 164, 382, 826, 1022, 1165, 1214, 1651, 1716
NlaIV GGNNCC 2 cut(s) 69, 1632
NmuCI GTSAC 4 cut(s) 424, 449, 1339, 1524
NruI TCGCGA 1 cut(s) 651
NsiI ATGCAT 3 cut(s) 380, 806, 1268
NspI RCATGY 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 1011
PceI AGGCCT 2 cut(s) 412, 1443
PciI ACATGT 1 cut(s) 1018
PciSI GCTCTTC 1 cut(s) 1395
PcsI WCGNNNNNNNCGW 1 cut(s) 1906
PctI GAATGC 3 cut(s) 380, 892, 1146
PdiI GCCGGC 1 cut(s) 2072
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 6 cut(s) 227, 646, 920, 1775, 1949, 2207
PfoI TCCNGGA 3 cut(s) 739, 1558, 1903
PkrI GCNGC 5 cut(s) 666, 1036, 1237, 1255, 2335
PleI GAGTC 2 cut(s) 710, 1733
PpsI GAGTC 2 cut(s) 710, 1733
PpuMI RGGWCCY 2 cut(s) 1138, 1631
PscI ACATGT 1 cut(s) 1018
Psp5II RGGWCCY 2 cut(s) 1138, 1631
Psp6I CCWGG 4 cut(s) 739, 1558, 1903, 2283
PspFI CCCAGC 1 cut(s) 978
PspGI CCWGG 4 cut(s) 739, 1558, 1903, 2283
PspN4I GGNNCC 2 cut(s) 69, 1632
PspPI GGNCC 8 cut(s) 172, 239, 734, 812, 1138, 1553, 1631, 2008
PspPPI RGGWCCY 2 cut(s) 1138, 1631
PstNI CAGNNNCTG 1 cut(s) 2333
PsuI RGATCY 1 cut(s) 2342
PvuII CAGCTG 1 cut(s) 982
RruI TCGCGA 1 cut(s) 651
RsaI GTAC 2 cut(s) 521, 2258
RsaNI GTAC 2 cut(s) 520, 2257
RseI CAYNNNNRTG 2 cut(s) 1215, 1261
SapI GCTCTTC 1 cut(s) 1395
SaqAI TTAA 4 cut(s) 492, 690, 758, 1455
SatI GCNGC 5 cut(s) 665, 1035, 1236, 1254, 2334
Sau3AI GATC 7 cut(s) 177, 1370, 1915, 2184, 2342, 2347, 2386
Sau96I GGNCC 8 cut(s) 172, 239, 734, 812, 1138, 1553, 1631, 2008
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 2 cut(s) 711, 1733
ScrFI CCNGG 8 cut(s) 131, 437, 512, 741, 787, 1560, 1905, 2285
SduI GDGCHC 1 cut(s) 1018
Sfr274I CTCGAG 1 cut(s) 1011
SinI GGWCC 7 cut(s) 172, 239, 734, 812, 1138, 1631, 2008
SlaI CTCGAG 1 cut(s) 1011
SmiMI CAYNNNNRTG 2 cut(s) 1215, 1261
SmlI CTYRAG 4 cut(s) 406, 1011, 1133, 1978
SmoI CTYRAG 4 cut(s) 406, 1011, 1133, 1978
Sse9I AATT 9 cut(s) 653, 759, 924, 957, 1434, 1505, 1514, 1612, 1683
SseBI AGGCCT 2 cut(s) 412, 1443
SsiI CCGC 6 cut(s) 58, 76, 433, 1144, 1566, 2251
SspI AATATT 1 cut(s) 1965
SspMI CTAG 3 cut(s) 290, 461, 1232
StuI AGGCCT 2 cut(s) 412, 1443
StyD4I CCNGG 8 cut(s) 129, 435, 510, 739, 785, 1558, 1903, 2283
StyI CCWWGG 3 cut(s) 1200, 1366, 1584
TaaI ACNGT 5 cut(s) 172, 524, 1402, 1477, 1846
TaiI ACGT 1 cut(s) 1912
TaqI TCGA 7 cut(s) 695, 870, 1012, 1182, 1446, 1689, 1728
TasI AATT 9 cut(s) 653, 759, 924, 957, 1434, 1505, 1514, 1612, 1683
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 6 cut(s) 227, 646, 920, 1775, 1949, 2207
Tru1I TTAA 4 cut(s) 492, 690, 758, 1455
Tru9I TTAA 4 cut(s) 492, 690, 758, 1455
TscAI CASTG 5 cut(s) 320, 1344, 1771, 2050, 2326
TseFI GTSAC 4 cut(s) 424, 449, 1339, 1524
TseI GCWGC 5 cut(s) 664, 1034, 1235, 1253, 2333
Tsp45I GTSAC 4 cut(s) 424, 449, 1339, 1524
TspGWI ACGGA 1 cut(s) 2340
TspRI CASTG 5 cut(s) 320, 1344, 1771, 2050, 2326
VpaK11BI GGWCC 7 cut(s) 172, 239, 734, 812, 1138, 1631, 2008
XagI CCTNNNNNAGG 3 cut(s) 179, 744, 1448
XapI RAATTY 3 cut(s) 1505, 1514, 1683
XceI RCATGY 1 cut(s) 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XhoI CTCGAG 1 cut(s) 1011
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 866
XspI CTAG 3 cut(s) 290, 461, 1232
ZraI GACGTC 1 cut(s) 1910
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 3 cut(s) 380, 806, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.