Rh6BG095700

Cell Division

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001165
Physical Location & Seq
Reverse (-)
14965013 .. 14967892
2880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG095700.1

Sequence Viewer

Length: 1059 bp
ATGTCGAACCAAGATGAGGGAACGAAGAAGGACTTCAGCACCGCCATTCTCAACCGCAAGAAGGCTCCAAATCGCCTCATTGTCGATGAAGCTGTGGTGGATGACAACTCCATCATCCAACTCCATCCGGCGACCATGGAGAAGCTCCAGCTCTTTCGTGGTGACACAGTCTTGATCAAAGGAAAGAAGAGAAGAGACACAATCTGTATTGCTCTTGCTGATGATACATGTGAGGAACCAAAGGTAAGGATGAACAAGGTTGTGAGGAACAACCTTAGGGTTAGGCTTGGTGATGTTGTTTCCATTCACCAATGCGCTGATGTTAAGTATGGAAAGCGTGTCCACATTCTCCCTATAGATGACACAATTGAAGGGATCACTGGAAATCTGTTTGATGCCTACTTGAAGCCTTATTTCCAAGAGACCTACCGCCCGGTGAGGAAAGGTGATCTTTTCCTAGTGAGAGGAGGAATGAGGAGTGTAGAGTTTAAGGTTATTGAGACTGACCCTCCTGAGTACTGTGTGGTTGCTTCGGACACCGAAATCTTCTGTGAGGGAGAACCTGTTAGAAGGGAGGATGAGGAAAGATTAGATGAGGTAGGGTATGATGATGTTGGTGGTGTTAGGAAACAGATGGCTCAGATTCGTGAATTGGTTGAGCTGCCTCTGAGGCATCCTCAGCTGTTTAAATCAATCGGTGTCAAACCACCAAAGGATGAAGTTGGGCGTCTTGAGGTCCTCCGCATTCATAGCAAGAACATGAAGCTTGCCGAAGATGTCGATTTAGAAAGAGTTGCCAAGGATACTCATGGGTATGTTGGTGCTGATCTAGCTGCTTTATCTACTGAGGCTGCACTTCAATGCATCCGAGAGAAGATGGATGTGATTGACTTGGAAGATGAGACAATAGATGCTGAGATACTCAACTCAATGGCAGTAACAAACGAACATTTCCAGACTGCTCTTGGAACAAGCAACCCTTCTGCTCTTCGTGAAACGGTTGTTGAAGTTCCCAATGTTACTTGGGATGCTATTGGAGGTAACTCGCGCAGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N

Protein Analysis

352

Amino Acids

39.64

Weight (kDa)

5.25

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 25 - 106 2e-22 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 126 - 190 9.1e-12 Cell division protein 48 (CDC48), domain 2
AAA_lid_3 PF17862 259 - 300 1.4e-14 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1048
AciI CCGC 4 cut(s) 42, 55, 430, 742
AclWI GGATC 1 cut(s) 383
AcuI CTGAAG 1 cut(s) 19
AcyI GRCGYC 1 cut(s) 727
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 2 cut(s) 16, 61
AflIII ACRYGT 1 cut(s) 227
AgsI TTSAA 4 cut(s) 371, 406, 860, 1007
AluBI AGCT 7 cut(s) 92, 145, 151, 661, 682, 766, 833
AluI AGCT 7 cut(s) 92, 145, 151, 661, 682, 766, 833
Alw26I GTCTC 4 cut(s) 189, 416, 494, 896
AlwI GGATC 1 cut(s) 383
ApeKI GCWGC 3 cut(s) 661, 833, 851
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 2 cut(s) 317, 1050
AspS9I GGNCC 1 cut(s) 736
AsuC2I CCSGG 1 cut(s) 434
AsuHPI GGTGA 5 cut(s) 173, 299, 302, 448, 458
AvaII GGWCC 1 cut(s) 736
AxyI CCTNAGG 1 cut(s) 275
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 3 cut(s) 648, 820, 838
BccI CCATC 4 cut(s) 119, 132, 628, 871
BciVI GTATCC 1 cut(s) 796
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 1 cut(s) 434
BcoDI GTCTC 4 cut(s) 189, 416, 494, 896
BfaI CTAG 2 cut(s) 458, 830
BfmI CTRYAG 1 cut(s) 354
BfuI GTATCC 1 cut(s) 796
BglI GCCNNNNNGGC 1 cut(s) 670
BisI GCNGC 3 cut(s) 662, 834, 852
BlsI GCNGC 3 cut(s) 663, 835, 853
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 1 cut(s) 434
Bme18I GGWCC 1 cut(s) 736
BmgT120I GGNCC 1 cut(s) 736
BmiI GGNNCC 2 cut(s) 66, 237
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 5 cut(s) 385, 682, 873, 901, 1018
BplI GAGNNNNNCTC 2 cut(s) 661, 693
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 752
BpuMI CCSGG 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 727
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 135, 798
BsaXI ACNNNNNCTCC 4 cut(s) 493, 523, 549, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 61
Bse1I ACTGG 1 cut(s) 385
Bse21I CCTNAGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 135, 798
BseLI CCNNNNNNNGG 2 cut(s) 16, 61
BseMII CTCAG 6 cut(s) 504, 653, 659, 692, 837, 906
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 480, 490
BseXI GCAGC 3 cut(s) 648, 820, 838
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 1048
BsiSI CCGG 2 cut(s) 128, 434
BslI CCNNNNNNNGG 2 cut(s) 16, 61
BsmAI GTCTC 4 cut(s) 189, 416, 494, 896
BsmI GAATGC 1 cut(s) 744
Bso31I GGTCTC 1 cut(s) 416
Bsp143I GATC 4 cut(s) 174, 375, 448, 826
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 4 cut(s) 42, 55, 430, 742
BspCNI CTCAG 6 cut(s) 505, 652, 660, 691, 838, 907
BspFNI CGCG 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 66, 237
BspPI GGATC 1 cut(s) 383
BspQI GCTCTTC 1 cut(s) 993
BspTNI GGTCTC 1 cut(s) 416
BsrI ACTGG 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 135, 798
BssMI GATC 4 cut(s) 174, 375, 448, 826
BssNI GRCGYC 1 cut(s) 727
BssT1I CCWWGG 2 cut(s) 135, 798
Bst4CI ACNGT 3 cut(s) 169, 521, 1000
Bst6I CTCTTC 3 cut(s) 182, 187, 993
BstACI GRCGYC 1 cut(s) 727
BstC8I GCNNGC 1 cut(s) 768
BstDEI CTNAG 7 cut(s) 275, 513, 639, 668, 678, 846, 915
BstDSI CCRYGG 1 cut(s) 135
BstFNI CGCG 1 cut(s) 1048
BstHHI GCGC 2 cut(s) 317, 1050
BstKTI GATC 4 cut(s) 177, 378, 451, 829
BstMAI GTCTC 4 cut(s) 189, 416, 494, 896
BstMBI GATC 4 cut(s) 174, 375, 448, 826
BstMWI GCNNNNNNNGC 4 cut(s) 670, 679, 750, 830
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 432
BstSFI CTRYAG 1 cut(s) 354
BstUI CGCG 1 cut(s) 1048
BstV1I GCAGC 3 cut(s) 648, 820, 838
Bsu36I CCTNAGG 1 cut(s) 275
BsuI GTATCC 1 cut(s) 796
BtgI CCRYGG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 768
CfoI GCGC 2 cut(s) 317, 1050
Cfr13I GGNCC 1 cut(s) 736
CseI GACGC 1 cut(s) 716
Csp6I GTAC 1 cut(s) 517
CviAII CATG 4 cut(s) 136, 228, 760, 809
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 7 cut(s) 275, 513, 639, 668, 678, 846, 915
DpnI GATC 4 cut(s) 176, 377, 450, 828
DpnII GATC 4 cut(s) 174, 375, 448, 826
DraI TTTAAA 1 cut(s) 688
Eam1104I CTCTTC 3 cut(s) 182, 187, 993
EarI CTCTTC 3 cut(s) 182, 187, 993
Eco130I CCWWGG 2 cut(s) 135, 798
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 1 cut(s) 736
Eco57I CTGAAG 1 cut(s) 19
Eco81I CCTNAGG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 736
EcoT14I CCWWGG 2 cut(s) 135, 798
EcoT22I ATGCAT 1 cut(s) 866
ErhI CCWWGG 2 cut(s) 135, 798
FaeI CATG 4 cut(s) 139, 231, 763, 812
FaiI YATR 9 cut(s) 137, 229, 330, 356, 606, 750, 761, 810, 816
FalI AAGNNNNNCTT 6 cut(s) 17, 49, 435, 467, 964, 996
FatI CATG 4 cut(s) 135, 227, 759, 808
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 662, 834, 852
Fsp4HI GCNGC 3 cut(s) 662, 834, 852
FspBI CTAG 2 cut(s) 458, 830
GlaI GCGC 2 cut(s) 316, 1049
GluI GCNGC 3 cut(s) 662, 834, 852
GsuI CTGGAG 1 cut(s) 131
HapII CCGG 2 cut(s) 128, 434
HgaI GACGC 1 cut(s) 716
HhaI GCGC 2 cut(s) 317, 1050
Hin1I GRCGYC 1 cut(s) 727
Hin1II CATG 4 cut(s) 139, 231, 763, 812
Hin6I GCGC 2 cut(s) 315, 1048
HinP1I GCGC 2 cut(s) 315, 1048
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 1 cut(s) 643
HpaII CCGG 2 cut(s) 128, 434
HphI GGTGA 5 cut(s) 173, 299, 302, 448, 458
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 4 cut(s) 535, 642, 669, 869
Hpy188III TCNNGA 6 cut(s) 172, 512, 647, 731, 955, 992
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 5 cut(s) 22, 55, 365, 564, 990
HpyCH4III ACNGT 3 cut(s) 169, 521, 1000
HpyCH4V TGCA 2 cut(s) 854, 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 670, 679, 750, 830
HpyF3I CTNAG 7 cut(s) 275, 513, 639, 668, 678, 846, 915
Hsp92I GRCGYC 1 cut(s) 727
Hsp92II CATG 4 cut(s) 139, 231, 763, 812
HspAI GCGC 2 cut(s) 315, 1048
Ksp22I TGATCA 1 cut(s) 174
Kzo9I GATC 4 cut(s) 174, 375, 448, 826
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 2 cut(s) 70, 150
LpnPI CCDG 7 cut(s) 141, 161, 366, 447, 525, 576, 968
Lsp1109I GCAGC 3 cut(s) 648, 820, 838
LweI GCATC 5 cut(s) 385, 682, 873, 901, 1018
MaeI CTAG 2 cut(s) 458, 830
MaeIII GTNAC 4 cut(s) 161, 937, 1018, 1040
MalI GATC 4 cut(s) 176, 377, 450, 828
MboI GATC 4 cut(s) 174, 375, 448, 826
MboII GAAGA 8 cut(s) 37, 199, 204, 538, 785, 886, 908, 980
MfeI CAATTG 1 cut(s) 366
MluCI AATT 2 cut(s) 366, 650
MmeI TCCRAC 1 cut(s) 142
Mph1103I ATGCAT 1 cut(s) 866
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 3 cut(s) 324, 489, 687
MslI CAYNNNNRTG 2 cut(s) 813, 859
MspA1I CMGCKG 1 cut(s) 682
MspI CCGG 2 cut(s) 128, 434
MspR9I CCNGG 1 cut(s) 434
MunI CAATTG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 744
MvnI CGCG 1 cut(s) 1048
MwoI GCNNNNNNNGC 4 cut(s) 670, 679, 750, 830
NciI CCSGG 1 cut(s) 434
NcoI CCATGG 1 cut(s) 135
NdeII GATC 4 cut(s) 174, 375, 448, 826
NlaIII CATG 4 cut(s) 139, 231, 763, 812
NlaIV GGNNCC 2 cut(s) 66, 237
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 866
NspI RCATGY 1 cut(s) 231
PciI ACATGT 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 993
PctI GAATGC 1 cut(s) 744
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 1 cut(s) 643
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 3 cut(s) 663, 835, 853
PpuMI RGGWCCY 1 cut(s) 736
PscI ACATGT 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 736
PspN4I GGNNCC 2 cut(s) 66, 237
PspPI GGNCC 1 cut(s) 736
PspPPI RGGWCCY 1 cut(s) 736
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 2 cut(s) 813, 859
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 3 cut(s) 324, 489, 687
SatI GCNGC 3 cut(s) 662, 834, 852
Sau3AI GATC 4 cut(s) 174, 375, 448, 826
Sau96I GGNCC 1 cut(s) 736
ScaI AGTACT 1 cut(s) 518
ScrFI CCNGG 1 cut(s) 434
SfaNI GCATC 5 cut(s) 385, 682, 873, 901, 1018
SfcI CTRYAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 736
SmiMI CAYNNNNRTG 2 cut(s) 813, 859
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 2 cut(s) 366, 650
SsiI CCGC 4 cut(s) 42, 55, 430, 742
SspMI CTAG 2 cut(s) 458, 830
StyD4I CCNGG 1 cut(s) 432
StyI CCWWGG 2 cut(s) 135, 798
TaaI ACNGT 3 cut(s) 169, 521, 1000
TaqI TCGA 3 cut(s) 5, 84, 780
TasI AATT 2 cut(s) 366, 650
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 3 cut(s) 324, 489, 687
Tru9I TTAA 3 cut(s) 324, 489, 687
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 3 cut(s) 661, 833, 851
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 5 cut(s) 102, 266, 732, 737, 776
TspRI CASTG 1 cut(s) 385
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 736
XceI RCATGY 1 cut(s) 231
XcmI CCANNNNNNNNNTGG 2 cut(s) 155, 962
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 458, 830
ZrmI AGTACT 1 cut(s) 518
Zsp2I ATGCAT 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.