Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001165

Physical Location & Seq

Genomic Coordinates Reverse (-)

13286751 .. 13291697

Gene Structure 4947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1142500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGAACAAGGTTGTCAGGTCAAATTTAAGGGTTCGACTTGGAGATGTTGTGTCTGTACATCAATGCCCTGATGTTAAGTATGGGAAGCGTGTTCACATTTTGCCTATTGATGACTCTATCGAAGGAGTTACAGGAAATCTCTTTGATGCCTTCTTGAAACCTTACTTCTTGGAAGCTTATCGCCCTGTGAGGAAAGGAGATCTGTTCCTTGTCAGAGGAGGAATGAGAAGTGTAGAGTTCAAGGTCATTGAAACTGACCCAGGAGAATATTGTGTGGTTGCCCCTGACACGGAGATCTTCTGTGAGGGGGAACCCGTGAAAAGGGAGGATGAGGAAAGGTTGGATGAAGTTGGTTATGATGACGTGGGTGGTGTTCGGAAGCAGATGGCTCAAATCCGTGAACTAGTAGAGCTTCCACTAAGGCATCCCCAACTCTTCAAATCAATTGGTGTTAAACCTCCGAAAGGAATTCTACTTTATGGACCCCCGGGATCAGGAAAGACATTAATAGCCAGAGCTGTTGCCAATGAAACTGGTGCTTTTTTCTTTTGCATTAATGGACCAGAGATTATGTCAAAATTGGCTGGAGAGAGTGAGAGCAATCTCAGAAAGGCATTTGAAGAAGCAGAGAAGAATGCTCCATCCATCATCTTTATCGATGAGATTGACTCAATTGCTCCTAAGCGGGAGAAGACACATGGTGAAGTTGAAAGGAGGATTGTCTCTCAGCTTTTGACTTTGATGGATGGACTGAAATCTCGAGCCCATGTTATCGTTATTGGTGCTACAAATCGTCCAAACAGTATTGACGCGGCTTTGAGAAGGTTTGGTAGGTTTGATAGGGAGATAGACATTGGTGTTCCTGATGAAGTGGGGCGCCTTGAAGTTCTCCGCATACACACAAAGAACATGAAACTCTCTGATGATGTTGATCTAGAGAGGATTTCCAAGGATACTCATGGGTATGTTGGTGCCGACCTTGCTGCTCTCTGTACTGAAGCTGCTCTGCAATGCATTAGGGAGAAGATGGATGTGATAGACTTGGAAGACGAATCCATTGATGCAGAGATACTTAATTCAATGGCTGTTACAAACGAGCACTTCCAGACTGCTCTTGGAACAAGCAATCCATCTGCTTTGCGTGAAACAGTTGTCGAAGTTCCTAATGTCAGTTGGGAGGATATTGGAGGCCTTGAGAATGTTAAGCGGGAGTTACAAGAGACCGTTCAATATCCTGTTGAGCACCCGGAGAAGTTTGAGAAATTTGGCATGTCACCTTCTAAAGGAGTACTTTTCTACGGGCCCCCAGGATGTGGGAAAACTCTTTTGGCCAAGGCGATTGCAAATGAATGTCAAGCTAACTTCATCAGTATTAAAGGCCCCGAACTGCTAACCATGTGGTTTGGTGAGAGTGAAGCCAATGTGCGAGAAATCTTTGACAAGGCTCGATCATCTGCACCGTGTGTCCTTTTCTTTGATGAGCTTGATTCCATTGCTACGCAGAGGGGAAGCAGTTCTGGGGATGCAGGTGGTGCTGCTGATCGTGTTCTTAACCAACTCCTGACTGAAATGGATGGCATGTCAGCAAAAAAGACTGTTTTCATTATTGGGGCCACAAACCGACCTGACATCATTGACCCAGCACTCCTACGCCCTGGCCGGCTTGACCAATTGATTTACATTCCTCTTCCAGATGAAGAATCACGTTTCCAAATATTTAAATCCTGCTTGAGAAAATCACCTGTTTCAAAGGATGTTGATCTGAGAGCACTGGCCAGATATACACAGGGCTTTAGTGGGGCCGATATCACAGAGATCTGCCAGCGGTCATGCAAGTATGCCATAAGAGAGAATATAGAAAAGGATATTGAGCGGGAGAGAAGGAGAGCAGAGAATCCAGATTCTATGGATGAGGACATTGACGATGAAGTGGCGGAGATTAAAGCTGCTCATTTTGAGGAGTCGATGAAATATGCTCGTAGGAGTGTCAGCGATGCTGATATACGCAAATACCAGGCCTTTGCTCAGACCTTGCAGCAGTCCAGGGGTTTCGGAACTGAGTTTAAGTTTACTGAAAATACACAAACTGGGACAACTGGTTCTGATGCATTCGCAACTTCTGCTGGTGCGGCTGATGAAGACGATTTGTATGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010038 GO:0010604 GO:0012505 GO:0016020 GO:0019222 GO:0031974 GO:0031981 GO:0042176 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046686 GO:0048518 GO:0050789 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0070013 GO:0071944 GO:0080090

KEGG Pathways

Metabolic & Signaling

ko04141 ko05134 map04141 map05134

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

719

Amino Acids

79.81

Weight (kDa)

5.01

Isoelectric Point (pI)

44.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_2	PF02933	43 - 107	6.3e-11	Cell division protein 48 (CDC48), domain 2
RuvB_N	PF05496	156 - 274	4.5e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA	PF00004	157 - 286	6.2e-48	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	309 - 350	6.2e-16	AAA+ lid domain
AAA	PF00004	430 - 563	1.1e-47	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	585 - 624	1.1e-11	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53230
fragaria_vesca	FvH4_2g06940 FvH4_2g06950 FvH4_2g07260 FvH4_4g17680 FvH4_6g16220 FvH4_6g16220
malus_domestica	MD03G1125700.v1.1 MD04G1094400.v1.1 MD11G1142500.v1.1 MD12G1114300.v1.1 MD17G1225300.v1.1 MD17G1225400.v1.1 MD17G1225600.v1.1 MD17G1228800.v1.1
prunus_persica	Prupe.4G237600_v2.0.a1 Prupe.6G107800_v2.0.a1 Prupe.6G233700_v2.0.a1 Prupe.6G233700_v2.0.a1
pyrus_communis	pycom04g08940 pycom04g08950 pycom09g14580 pycom11g11820 pycom12g11360
rosa_chinensis	RchiOBHm_Chr3g0470061 RchiOBHm_Chr4g0421621 RchiOBHm_Chr6g0259281 RchiOBHm_Chr6g0259871
rosa_laevigata	RLG00000007663 RLG00000014503 RLG00000014543 RLG00000024300
rosa_multiflora	Rmu_co8522155.1_g000003 Rmu_sc0005600.1_g000001 Rmu_sc0009049.1_g000005 Rmu_sc0011974.1_g000005 Rmu_sc0033604.1_g000001 Rmu_sc0033605.1_g000001 Rmu_ssc0000050.1_g000089
rosa_roxburghii	Rroxscaffold_5G00363870 Rroxscaffold_6G00411220
rosa_rugosa	Rorug03G0106600 Rorug04G0173900 Rorug05G0586800 Rorug05G0590900
rosa_samantha	Rh3AG155800 Rh4CG248200 Rh6AG103300 Rh6AG103400 Rh6AG107600 Rh6BG095700 Rh6CG092600 Rh6CG096400
rosa_wichuraiana	Rw3G014670 Rw4G020040 Rw6G008920 Rw6G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1517
Acc36I	ACCTGC	1 cut(s)	1517
AccB1I	GGYRCC	2 cut(s)	876, 971
AccB7I	CCANNNNNTGG	1 cut(s)	1313
AccBSI	CCGCTC	1 cut(s)	1873
AccII	CGCG	1 cut(s)	812
AciI	CCGC	8 cut(s)	685, 812, 892, 1207, 1825, 1873, 1934, 2129
AclWI	GGATC	1 cut(s)	499
AcoI	YGGCCR	3 cut(s)	1329, 1657, 1773
AcsI	RAATTY	3 cut(s)	22, 468, 1262
AcuI	CTGAAG	1 cut(s)	1017
AcyI	GRCGYC	1 cut(s)	877
AdeI	CACNNNGTG	2 cut(s)	701, 1463
AfaI	GTAC	3 cut(s)	57, 994, 1290
AfiI	CCNNNNNNNGG	6 cut(s)	289, 321, 464, 494, 1283, 1313
AhlI	ACTAGT	1 cut(s)	403
AjiI	CACGTC	1 cut(s)	364
AjnI	CCWGG	5 cut(s)	259, 1306, 1654, 2013, 2042
AjuI	GAANNNNNNNTTGG	2 cut(s)	1310, 1342
AloI	GAACNNNNNNTCC	4 cut(s)	1111, 1143, 2083, 2115
AluBI	AGCT	8 cut(s)	176, 412, 518, 730, 1001, 1358, 1483, 1946
AluI	AGCT	8 cut(s)	176, 412, 518, 730, 1001, 1358, 1483, 1946
Alw21I	GWGCWC	3 cut(s)	1101, 1245, 1771
Alw26I	GTCTC	2 cut(s)	727, 1214
AlwI	GGATC	1 cut(s)	499
Ama87I	CYCGRG	2 cut(s)	487, 759
AoxI	GGCC	9 cut(s)	1189, 1301, 1329, 1378, 1611, 1657, 1773, 1800, 2016
ApaI	GGGCCC	1 cut(s)	1305
ApeKI	GCWGC	5 cut(s)	983, 1001, 1535, 1946, 2035
ApoI	RAATTY	3 cut(s)	22, 468, 1262
AseI	ATTAAT	2 cut(s)	506, 555
Asp700I	GAANNNNTTC	1 cut(s)	1513
AspLEI	GCGC	1 cut(s)	879
AspS9I	GGNCC	7 cut(s)	482, 560, 1301, 1302, 1379, 1611, 1800
AsuC2I	CCSGG	3 cut(s)	488, 489, 1247
AsuHPI	GGTGA	4 cut(s)	713, 1266, 1418, 1731
AvaI	CYCGRG	2 cut(s)	487, 759
AvaII	GGWCC	2 cut(s)	482, 560
BaeGI	GKGCMC	1 cut(s)	1305
BalI	TGGCCA	2 cut(s)	1331, 1775
BanI	GGYRCC	2 cut(s)	876, 971
BanII	GRGCYC	2 cut(s)	766, 1305
BbsI	GAAGAC	3 cut(s)	698, 1053, 2145
Bbv12I	GWGCWC	3 cut(s)	1101, 1245, 1771
BbvI	GCAGC	5 cut(s)	970, 988, 1522, 1933, 2047
BccI	CCATC	8 cut(s)	379, 649, 653, 736, 740, 1021, 1138, 1568
BcgI	CGANNNNNNTGC	2 cut(s)	965, 999
BciT130I	CCWGG	5 cut(s)	261, 1308, 1656, 2015, 2044
BciVI	GTATCC	1 cut(s)	946
BcnI	CCSGG	3 cut(s)	488, 489, 1247
BcoDI	GTCTC	2 cut(s)	727, 1214
BcuI	ACTAGT	1 cut(s)	403
BfaI	CTAG	2 cut(s)	404, 935
BfoI	RGCGCY	1 cut(s)	880
BfuAI	ACCTGC	1 cut(s)	1517
BfuI	GTATCC	1 cut(s)	946
BglII	AGATCT	3 cut(s)	199, 294, 1815
BisI	GCNGC	7 cut(s)	813, 984, 1002, 1536, 1947, 2036, 2130
BlsI	GCNGC	7 cut(s)	814, 985, 1003, 1537, 1948, 2037, 2131
BmcAI	AGTACT	1 cut(s)	1290
Bme1390I	CCNGG	8 cut(s)	261, 488, 489, 1247, 1308, 1656, 2015, 2044
Bme18I	GGWCC	2 cut(s)	482, 560
BmeT110I	CYCGRG	2 cut(s)	487, 759
BmgBI	CACGTC	1 cut(s)	364
BmgT120I	GGNCC	7 cut(s)	482, 560, 1301, 1302, 1379, 1611, 1800
BmiI	GGNNCC	9 cut(s)	312, 484, 878, 973, 1303, 1304, 1381, 1612, 1801
BmrFI	CCNGG	8 cut(s)	261, 488, 489, 1247, 1308, 1656, 2015, 2044
BmrI	ACTGGG	1 cut(s)	2097
BmsI	GCATC	6 cut(s)	136, 433, 1051, 1513, 1984, 2095
BmuI	ACTGGG	1 cut(s)	2097
BpiI	GAAGAC	3 cut(s)	698, 1053, 2145
BplI	GAGNNNNNCTC	2 cut(s)	653, 685
BpmI	CTGGAG	1 cut(s)	606
Bpu10I	CCTNAGC	1 cut(s)	681
BpuEI	CTTGAG	2 cut(s)	1214, 1750
BpuMI	CCSGG	3 cut(s)	488, 489, 1247
Bsa29I	ATCGAT	1 cut(s)	657
BsaBI	GATNNNNATC	2 cut(s)	930, 1758
BsaHI	GRCGYC	1 cut(s)	877
BsaI	GGTCTC	1 cut(s)	1214
BsaJI	CCNNGG	8 cut(s)	259, 486, 487, 948, 1306, 1332, 1654, 2043
BsaXI	ACNNNNNCTCC	2 cut(s)	33, 63
Bsc4I	CCNNNNNNNGG	6 cut(s)	289, 321, 464, 494, 1283, 1313
Bse118I	RCCGGY	1 cut(s)	1659
Bse1I	ACTGG	4 cut(s)	538, 1776, 2092, 2101
Bse3DI	GCAATG	2 cut(s)	1016, 1491
Bse8I	GATNNNNATC	2 cut(s)	930, 1758
BseBI	CCWGG	5 cut(s)	261, 1308, 1656, 2015, 2044
BseCI	ATCGAT	1 cut(s)	657
BseDI	CCNNGG	8 cut(s)	259, 486, 487, 948, 1306, 1332, 1654, 2043
BseJI	GATNNNNATC	2 cut(s)	930, 1758
BseLI	CCNNNNNNNGG	6 cut(s)	289, 321, 464, 494, 1283, 1313
BseMI	GCAATG	2 cut(s)	1016, 1491
BseMII	CTCAG	5 cut(s)	619, 740, 1754, 2039, 2049
BseNI	ACTGG	4 cut(s)	538, 1776, 2092, 2101
BseRI	GAGGAG	2 cut(s)	231, 1973
BseSI	GKGCMC	1 cut(s)	1305
BseXI	GCAGC	5 cut(s)	970, 988, 1522, 1933, 2047
BseYI	CCCAGC	1 cut(s)	1639
BsgI	GTGCAG	1 cut(s)	1440
Bsh1236I	CGCG	1 cut(s)	812
BshFI	GGCC	9 cut(s)	1191, 1303, 1331, 1380, 1613, 1659, 1775, 1802, 2018
BshNI	GGYRCC	2 cut(s)	876, 971
BshVI	ATCGAT	1 cut(s)	657
BsiHKAI	GWGCWC	3 cut(s)	1101, 1245, 1771
BsiHKCI	CYCGRG	2 cut(s)	487, 759
BsiSI	CCGG	3 cut(s)	488, 1247, 1660
BslFI	GGGAC	1 cut(s)	2104
BslI	CCNNNNNNNGG	6 cut(s)	289, 321, 464, 494, 1283, 1313
BsmAI	GTCTC	2 cut(s)	727, 1214
BsmFI	GGGAC	1 cut(s)	2104
BsmI	GAATGC	2 cut(s)	640, 2108
BsnI	GGCC	9 cut(s)	1191, 1303, 1331, 1380, 1613, 1659, 1775, 1802, 2018
Bso31I	GGTCTC	1 cut(s)	1214
BsoBI	CYCGRG	2 cut(s)	487, 759
Bsp120I	GGGCCC	1 cut(s)	1301
Bsp1286I	GDGCHC	5 cut(s)	766, 1101, 1245, 1305, 1771
Bsp1407I	TGTACA	1 cut(s)	55
Bsp143I	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
BspACI	CCGC	8 cut(s)	685, 812, 892, 1207, 1825, 1873, 1934, 2129
BspANI	GGCC	9 cut(s)	1191, 1303, 1331, 1380, 1613, 1659, 1775, 1802, 2018
BspCNI	CTCAG	5 cut(s)	618, 739, 1755, 2038, 2050
BspDI	ATCGAT	1 cut(s)	657
BspFNI	CGCG	1 cut(s)	812
BspLI	GGNNCC	9 cut(s)	312, 484, 878, 973, 1303, 1304, 1381, 1612, 1801
BspMI	ACCTGC	1 cut(s)	1517
BspPI	GGATC	1 cut(s)	499
BspT107I	GGYRCC	2 cut(s)	876, 971
BspTNI	GGTCTC	1 cut(s)	1214
BsrBI	CCGCTC	1 cut(s)	1873
BsrDI	GCAATG	2 cut(s)	1016, 1491
BsrFI	RCCGGY	1 cut(s)	1659
BsrGI	TGTACA	1 cut(s)	55
BsrI	ACTGG	4 cut(s)	538, 1776, 2092, 2101
BssAI	RCCGGY	1 cut(s)	1659
BssECI	CCNNGG	8 cut(s)	259, 486, 487, 948, 1306, 1332, 1654, 2043
BssMI	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
BssNI	GRCGYC	1 cut(s)	877
BssT1I	CCWWGG	2 cut(s)	948, 1332
Bst2UI	CCWGG	5 cut(s)	261, 1308, 1656, 2015, 2044
Bst4CI	ACNGT	5 cut(s)	803, 1150, 1225, 1461, 1597
Bst6I	CTCTTC	2 cut(s)	440, 1692
BstACI	GRCGYC	1 cut(s)	877
BstAPI	GCANNNNNTGC	2 cut(s)	1532, 2120
BstAUI	TGTACA	1 cut(s)	55
BstC8I	GCNNGC	2 cut(s)	1661, 1823
BstDEI	CTNAG	7 cut(s)	419, 605, 681, 726, 1763, 2025, 2058
BstENI	CCTNNNNNAGG	2 cut(s)	462, 1281
BstFNI	CGCG	1 cut(s)	812
BstH2I	RGCGCY	1 cut(s)	880
BstHHI	GCGC	1 cut(s)	879
BstKTI	GATC	8 cut(s)	202, 297, 494, 934, 1451, 1543, 1762, 1818
BstMAI	GTCTC	2 cut(s)	727, 1214
BstMBI	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
BstMWI	GCNNNNNNNGC	4 cut(s)	980, 1532, 2120, 2129
BstNI	CCWGG	5 cut(s)	261, 1308, 1656, 2015, 2044
BstNSI	RCATGY	2 cut(s)	1273, 1582
BstSCI	CCNGG	8 cut(s)	259, 486, 487, 1245, 1306, 1654, 2013, 2042
BstSLI	GKGCMC	1 cut(s)	1305
BstUI	CGCG	1 cut(s)	812
BstV1I	GCAGC	5 cut(s)	970, 988, 1522, 1933, 2047
BstV2I	GAAGAC	3 cut(s)	698, 1053, 2145
BstX2I	RGATCY	3 cut(s)	199, 294, 1815
BstYI	RGATCY	3 cut(s)	199, 294, 1815
Bsu15I	ATCGAT	1 cut(s)	657
BsuI	GTATCC	1 cut(s)	946
BsuRI	GGCC	9 cut(s)	1191, 1303, 1331, 1380, 1613, 1659, 1775, 1802, 2018
BsuTUI	ATCGAT	1 cut(s)	657
BtgZI	GCGATG	1 cut(s)	2007
BtrI	CACGTC	1 cut(s)	364
BtsIMutI	CAGTG	1 cut(s)	1769
BveI	ACCTGC	1 cut(s)	1517
Cac8I	GCNNGC	2 cut(s)	1661, 1823
CfoI	GCGC	1 cut(s)	879
Cfr10I	RCCGGY	1 cut(s)	1659
Cfr13I	GGNCC	7 cut(s)	482, 560, 1301, 1302, 1379, 1611, 1800
Cfr9I	CCCGGG	1 cut(s)	487
ClaI	ATCGAT	1 cut(s)	657
CseI	GACGC	1 cut(s)	818
Csp6I	GTAC	3 cut(s)	56, 993, 1289
CviAII	CATG	8 cut(s)	698, 767, 910, 959, 1270, 1396, 1579, 1830
CviQI	GTAC	3 cut(s)	56, 993, 1289
DdeI	CTNAG	7 cut(s)	419, 605, 681, 726, 1763, 2025, 2058
DinI	GGCGCC	1 cut(s)	878
DpnI	GATC	8 cut(s)	201, 296, 493, 933, 1450, 1542, 1761, 1817
DpnII	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
DraI	TTTAAA	1 cut(s)	1720
DraIII	CACNNNGTG	2 cut(s)	701, 1463
EaeI	YGGCCR	3 cut(s)	1329, 1657, 1773
Eam1104I	CTCTTC	2 cut(s)	440, 1692
EarI	CTCTTC	2 cut(s)	440, 1692
EciI	GGCGGA	1 cut(s)	1949
Eco130I	CCWWGG	2 cut(s)	948, 1332
Eco147I	AGGCCT	2 cut(s)	1191, 2018
Eco24I	GRGCYC	2 cut(s)	766, 1305
Eco31I	GGTCTC	1 cut(s)	1214
Eco32I	GATATC	1 cut(s)	1807
Eco47I	GGWCC	2 cut(s)	482, 560
Eco57I	CTGAAG	1 cut(s)	1017
Eco88I	CYCGRG	2 cut(s)	487, 759
EcoNI	CCTNNNNNAGG	2 cut(s)	462, 1281
EcoO109I	RGGNCCY	2 cut(s)	1302, 1379
EcoRI	GAATTC	1 cut(s)	468
EcoRII	CCWGG	5 cut(s)	259, 1306, 1654, 2013, 2042
EcoRV	GATATC	1 cut(s)	1807
EcoT14I	CCWWGG	2 cut(s)	948, 1332
EcoT22I	ATGCAT	2 cut(s)	1016, 2110
EcoT38I	GRGCYC	2 cut(s)	766, 1305
EgeI	GGCGCC	1 cut(s)	878
EheI	GGCGCC	1 cut(s)	878
ErhI	CCWWGG	2 cut(s)	948, 1332
FaeI	CATG	8 cut(s)	701, 770, 913, 962, 1273, 1399, 1582, 1833
FalI	AAGNNNNNCTT	2 cut(s)	1275, 1307
FaqI	GGGAC	1 cut(s)	2104
FatI	CATG	8 cut(s)	697, 766, 909, 958, 1269, 1395, 1578, 1829
FauI	CCCGC	3 cut(s)	678, 1200, 1866
Fnu4HI	GCNGC	7 cut(s)	813, 984, 1002, 1536, 1947, 2036, 2130
FriOI	GRGCYC	2 cut(s)	766, 1305
Fsp4HI	GCNGC	7 cut(s)	813, 984, 1002, 1536, 1947, 2036, 2130
FspBI	CTAG	2 cut(s)	404, 935
GlaI	GCGC	1 cut(s)	878
GluI	GCNGC	7 cut(s)	813, 984, 1002, 1536, 1947, 2036, 2130
GsaI	CCCAGC	1 cut(s)	1643
GsuI	CTGGAG	1 cut(s)	606
HaeII	RGCGCY	1 cut(s)	880
HaeIII	GGCC	9 cut(s)	1191, 1303, 1331, 1380, 1613, 1659, 1775, 1802, 2018
HapII	CCGG	3 cut(s)	488, 1247, 1660
HgaI	GACGC	1 cut(s)	818
HhaI	GCGC	1 cut(s)	879
Hin1I	GRCGYC	1 cut(s)	877
Hin1II	CATG	8 cut(s)	701, 770, 913, 962, 1273, 1399, 1582, 1833
Hin6I	GCGC	1 cut(s)	877
HinP1I	GCGC	1 cut(s)	877
HindIII	AAGCTT	1 cut(s)	174
HinfI	GANTC	8 cut(s)	113, 668, 1052, 1487, 1700, 1894, 1901, 1961
HpaII	CCGG	3 cut(s)	488, 1247, 1660
HphI	GGTGA	4 cut(s)	713, 1266, 1418, 1731
Hpy166II	GTNNAC	3 cut(s)	94, 401, 2070
Hpy188I	TCNGA	9 cut(s)	215, 378, 462, 608, 922, 1764, 2028, 2054, 2104
Hpy188III	TCNNGA	9 cut(s)	154, 495, 759, 863, 935, 1105, 1561, 1691, 1898
Hpy8I	GTNNAC	3 cut(s)	94, 401, 2070
HpyAV	CCTTC	5 cut(s)	116, 160, 816, 1287, 1875
HpyCH4III	ACNGT	5 cut(s)	803, 1150, 1225, 1461, 1597
HpyCH4IV	ACGT	2 cut(s)	363, 1705
HpyF10VI	GCNNNNNNNGC	4 cut(s)	980, 1532, 2120, 2129
HpyF3I	CTNAG	7 cut(s)	419, 605, 681, 726, 1763, 2025, 2058
HpySE526I	ACGT	2 cut(s)	363, 1705
Hsp92I	GRCGYC	1 cut(s)	877
Hsp92II	CATG	8 cut(s)	701, 770, 913, 962, 1273, 1399, 1582, 1833
HspAI	GCGC	1 cut(s)	877
KasI	GGCGCC	1 cut(s)	876
KroI	GCCGGC	1 cut(s)	1659
KroNI	GCCGGC	1 cut(s)	1661
Kzo9I	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
LmnI	GCTCC	2 cut(s)	643, 682
Lsp1109I	GCAGC	5 cut(s)	970, 988, 1522, 1933, 2047
LweI	GCATC	6 cut(s)	136, 433, 1051, 1513, 1984, 2095
MaeI	CTAG	2 cut(s)	404, 935
MaeII	ACGT	2 cut(s)	363, 1705
MaeIII	GTNAC	4 cut(s)	127, 1087, 1212, 1272
MalI	GATC	8 cut(s)	201, 296, 493, 933, 1450, 1542, 1761, 1817
MbiI	CCGCTC	1 cut(s)	1873
MboI	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
MfeI	CAATTG	3 cut(s)	444, 672, 1670
MflI	RGATCY	3 cut(s)	199, 294, 1815
MhlI	GDGCHC	5 cut(s)	766, 1101, 1245, 1305, 1771
MlsI	TGGCCA	2 cut(s)	1331, 1775
MluCI	AATT	8 cut(s)	22, 444, 468, 578, 672, 1075, 1262, 1670
MluNI	TGGCCA	2 cut(s)	1331, 1775
Mly113I	GGCGCC	1 cut(s)	877
MlyI	GAGTC	3 cut(s)	107, 662, 1970
MmeI	TCCRAC	1 cut(s)	321
Mox20I	TGGCCA	2 cut(s)	1331, 1775
Mph1103I	ATGCAT	2 cut(s)	1016, 2110
MroNI	GCCGGC	1 cut(s)	1659
MroXI	GAANNNNTTC	1 cut(s)	1513
MscI	TGGCCA	2 cut(s)	1331, 1775
MslI	CAYNNNNRTG	1 cut(s)	963
Msp20I	TGGCCA	2 cut(s)	1331, 1775
MspA1I	CMGCKG	1 cut(s)	1825
MspI	CCGG	3 cut(s)	488, 1247, 1660
MspR9I	CCNGG	8 cut(s)	261, 488, 489, 1247, 1308, 1656, 2015, 2044
MunI	CAATTG	3 cut(s)	444, 672, 1670
Mva1269I	GAATGC	2 cut(s)	640, 2108
MvaI	CCWGG	5 cut(s)	261, 1308, 1656, 2015, 2044
MvnI	CGCG	1 cut(s)	812
MwoI	GCNNNNNNNGC	4 cut(s)	980, 1532, 2120, 2129
NaeI	GCCGGC	1 cut(s)	1661
NarI	GGCGCC	1 cut(s)	877
NciI	CCSGG	3 cut(s)	488, 489, 1247
NdeII	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
NgoMIV	GCCGGC	1 cut(s)	1659
NlaIII	CATG	8 cut(s)	701, 770, 913, 962, 1273, 1399, 1582, 1833
NlaIV	GGNNCC	9 cut(s)	312, 484, 878, 973, 1303, 1304, 1381, 1612, 1801
NmuCI	GTSAC	1 cut(s)	1272
NsiI	ATGCAT	2 cut(s)	1016, 2110
NspI	RCATGY	2 cut(s)	1273, 1582
PaeR7I	CTCGAG	1 cut(s)	759
PaqCI	CACCTGC	1 cut(s)	1517
PceI	AGGCCT	2 cut(s)	1191, 2018
PctI	GAATGC	2 cut(s)	640, 2108
PdiI	GCCGGC	1 cut(s)	1661
PdmI	GAANNNNTTC	1 cut(s)	1513
PfeI	GAWTC	5 cut(s)	1052, 1487, 1700, 1894, 1901
PflMI	CCANNNNNTGG	1 cut(s)	1313
PkrI	GCNGC	7 cut(s)	814, 985, 1003, 1537, 1948, 2037, 2131
PleI	GAGTC	3 cut(s)	107, 662, 1969
PluTI	GGCGCC	1 cut(s)	880
PpsI	GAGTC	3 cut(s)	107, 662, 1969
PshBI	ATTAAT	2 cut(s)	506, 555
Psp6I	CCWGG	5 cut(s)	259, 1306, 1654, 2013, 2042
PspFI	CCCAGC	1 cut(s)	1639
PspGI	CCWGG	5 cut(s)	259, 1306, 1654, 2013, 2042
PspN4I	GGNNCC	9 cut(s)	312, 484, 878, 973, 1303, 1304, 1381, 1612, 1801
PspOMI	GGGCCC	1 cut(s)	1301
PspPI	GGNCC	7 cut(s)	482, 560, 1301, 1302, 1379, 1611, 1800
PsuI	RGATCY	3 cut(s)	199, 294, 1815
RsaI	GTAC	3 cut(s)	57, 994, 1290
RsaNI	GTAC	3 cut(s)	56, 993, 1289
RseI	CAYNNNNRTG	1 cut(s)	963
SatI	GCNGC	7 cut(s)	813, 984, 1002, 1536, 1947, 2036, 2130
Sau3AI	GATC	8 cut(s)	199, 294, 491, 931, 1448, 1540, 1759, 1815
Sau96I	GGNCC	7 cut(s)	482, 560, 1301, 1302, 1379, 1611, 1800
ScaI	AGTACT	1 cut(s)	1290
SchI	GAGTC	3 cut(s)	107, 662, 1970
ScrFI	CCNGG	8 cut(s)	261, 488, 489, 1247, 1308, 1656, 2015, 2044
SduI	GDGCHC	5 cut(s)	766, 1101, 1245, 1305, 1771
SfaNI	GCATC	6 cut(s)	136, 433, 1051, 1513, 1984, 2095
SfoI	GGCGCC	1 cut(s)	878
Sfr274I	CTCGAG	1 cut(s)	759
SinI	GGWCC	2 cut(s)	482, 560
SlaI	CTCGAG	1 cut(s)	759
SmaI	CCCGGG	1 cut(s)	489
SmiI	ATTTAAAT	1 cut(s)	1720
SmiMI	CAYNNNNRTG	1 cut(s)	963
SmlI	CTYRAG	3 cut(s)	759, 1193, 1729
SmoI	CTYRAG	3 cut(s)	759, 1193, 1729
SpeI	ACTAGT	1 cut(s)	403
Sse9I	AATT	8 cut(s)	22, 444, 468, 578, 672, 1075, 1262, 1670
SseBI	AGGCCT	2 cut(s)	1191, 2018
SsiI	CCGC	8 cut(s)	685, 812, 892, 1207, 1825, 1873, 1934, 2129
SspDI	GGCGCC	1 cut(s)	876
SspI	AATATT	2 cut(s)	269, 1716
SspMI	CTAG	2 cut(s)	404, 935
StuI	AGGCCT	2 cut(s)	1191, 2018
StyD4I	CCNGG	8 cut(s)	259, 486, 487, 1245, 1306, 1654, 2013, 2042
StyI	CCWWGG	2 cut(s)	948, 1332
SwaI	ATTTAAAT	1 cut(s)	1720
TaaI	ACNGT	5 cut(s)	803, 1150, 1225, 1461, 1597
TaiI	ACGT	2 cut(s)	366, 1708
TaqI	TCGA	7 cut(s)	34, 120, 657, 760, 1155, 1447, 1964
TasI	AATT	8 cut(s)	22, 444, 468, 578, 672, 1075, 1262, 1670
TatI	WGTACW	3 cut(s)	55, 992, 1288
TauI	GCSGC	2 cut(s)	815, 2132
TfiI	GAWTC	5 cut(s)	1052, 1487, 1700, 1894, 1901
TscAI	CASTG	1 cut(s)	1776
TseFI	GTSAC	1 cut(s)	1272
TseI	GCWGC	5 cut(s)	983, 1001, 1535, 1946, 2035
Tsp45I	GTSAC	1 cut(s)	1272
TspGWI	ACGGA	2 cut(s)	305, 386
TspMI	CCCGGG	1 cut(s)	487
TspRI	CASTG	1 cut(s)	1776
Van91I	CCANNNNNTGG	1 cut(s)	1313
VpaK11BI	GGWCC	2 cut(s)	482, 560
VspI	ATTAAT	2 cut(s)	506, 555
XagI	CCTNNNNNAGG	2 cut(s)	462, 1281
XapI	RAATTY	3 cut(s)	22, 468, 1262
XbaI	TCTAGA	1 cut(s)	934
XceI	RCATGY	2 cut(s)	1273, 1582
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1112
XhoI	CTCGAG	1 cut(s)	759
XmaI	CCCGGG	1 cut(s)	487
XmnI	GAANNNNTTC	1 cut(s)	1513
XspI	CTAG	2 cut(s)	404, 935
ZrmI	AGTACT	1 cut(s)	1290
Zsp2I	ATGCAT	2 cut(s)	1016, 2110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.