Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0001165

Physical Location & Seq

Genomic Coordinates Reverse (-)

19583183 .. 19585072

Gene Structure 1890 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000007663

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGGCTCTCCAATCTGCTCTGCCATTTGAAATGTACAACCAATTCGCCGATCAATCCTCCAATTCCAATAAAGCCACAAAGAGGGACTTCAGCAATGTCCACGGTTCCGATGATGGCGACGCAATCCTGGTCAATGCAATGAAAAGAATCAAGCTCTCATCAGATAATGGTGACTCATCTCAGATGAAGACGAAGAAGAAGGAAATTGGGTATGAAGACGTAGGTGGTGTGAGGAAGCAGATGGCTCAGATTCGTGAGTTAGTGGAGCTTCCATTGAAGCAACCCAAGTTGTTTAAGCTCATTGGAGTGAAGCCTCCAAAGGGTATTCTTCTATACGGGCCCCCTGGCTCGGGCAAGACTCTGATTGCCCGAGCCATCGCGAACGAGACGGGGGCCTTGTTTTTGTGCATTAATGGGCCGGAGATCATGTCGAAAATGGCTGGGGAGAGTGAGAGCAATCTAAGGAAGGTTTTCGAGGAAGCGGGGAAGAATGCACCGTCTATTGTGTTTATAGATGAGATTGACACGATTGCTCCGAAGAGAGAGAAGAGTAATGGGGAAGTTGAGAGGAGGATTGTTTCACAGCTCTTGACACTCATGGATGGTATGAAGTCAAAAGGGCGTGTGGTTGTTATTGGGGCAACCAATCGGCCTAATAGTATTGACCCAGCTCTTAGGAGGTGTGGAAGATTTGATAGGGAGATAGATATTGGTGTTCCAGATGAGGCCGGGCGGCTTGAGGTGCTTCGAATCCATACCAAGAACATGAAGCTTGGTGAGGATGTGGAGTTGGAAAAGATAGCTAAGGAGACACATGGGTGTGTTGGGGCAGATTTAGCTGCATTGTGCACTGAGGCTGCGCTTCAATGCATAAGAGAGAAAATGGATGAAATTGACTGGGAAGATCAGGAGACATTAGATGCTGAGATGCTTGGCTCTATGAAAGTTGGAAATGAGCACTTTCAGACTGCTTTGCGAGCAAGCAACCCTTCTGCTCTTCGCGAAACGGCTGTGGAGGTGCCGAATGTGACTTGGGATGCTATCGGGGGTCTGGAGAAAGTGAAGAGGGAACTTCAGGAGACTATTCAGTATCCAGTTGAGCATCCTGAGAAGTTTGAGAAATATGGAATGTCACCCTCAAAGGGGGTTCTTTTCTATGGCCCTCCTGGGTGTGGTAAGACCCTTTTGGCCAAGGCTATTGCTAATGAGTGTCAGGCAAATTTTATTAGTATCAAGGGGCCTGAGTTGCTTACTAGTGGGTTTGGAGAGAGTGAGGCGAATGTGAGGGAAATTTTCGACAAGGCTAGAGGGTCTGCTCCCTGTGTCCTCTTTTTTGATGAACTCGATTCCATTGCTACTCAGAGAAGTAGTAATGCTGGAGGTGGTGCTGCTGCTGATAGGGTTTTGAACCAACTCCTTACAGAAATGGATGGATTGTCTGCTAAGAAAACTGTCTTTGTTATCGGGGCCACCAATAGACCTGACATTATAGACCCTGCACTTCTCCGTCCAGGACGTCTGGACCAATTGATTTACATCCCTCTCCCTGATGAGGAATCCCGCTGTGAAATATTCAAGGCTTGCTTGAGGAAGTCTCCAGTTTCCAAAGATGTGAACATCAGAGCTCTTGCAAAGAACACTAGAGGATTTAGTGGTGCTGATATCACAGAAATATGCCAGCGTGCTTGCAAATATGCCATTAGAGAGAACATTGGGAATGATATCAAGAGGGAGATGAGCAGAAGTGAGAATTGTGAAGATATGGAGGAGGATGAAGTGGCAGGGATCACGGCAGCTCACTTCGTGGAGTCGATGAAGTATGCTCGTAGAAGTGTCATTGATTCCGATATCCGCAAATACAAGGCATATGCAGACACTGCAGCAGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005794 GO:0005829 GO:0005886 GO:0008150 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010038 GO:0010604 GO:0012505 GO:0016020 GO:0019222 GO:0031974 GO:0031981 GO:0042176 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0046686 GO:0048518 GO:0050789 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0070013 GO:0071944 GO:0080090

KEGG Pathways

Metabolic & Signaling

ko04141 ko05134 map04141 map05134

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N Vps4_C

Protein Analysis

630

Amino Acids

69.09

Weight (kDa)

5.68

Isoelectric Point (pI)

44.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RuvB_N	PF05496	108 - 182	4.5e-06	Holliday junction DNA helicase RuvB P-loop domain
AAA	PF00004	109 - 238	6.7e-48	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	261 - 304	1.1e-16	AAA+ lid domain
AAA	PF00004	383 - 515	3.8e-46	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	537 - 573	1e-11	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001165)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53230
fragaria_vesca	FvH4_2g06940 FvH4_2g06950 FvH4_2g07260 FvH4_4g17680 FvH4_6g16220 FvH4_6g16220
malus_domestica	MD03G1125700.v1.1 MD04G1094400.v1.1 MD11G1142500.v1.1 MD12G1114300.v1.1 MD17G1225300.v1.1 MD17G1225400.v1.1 MD17G1225600.v1.1 MD17G1228800.v1.1
prunus_persica	Prupe.4G237600_v2.0.a1 Prupe.6G107800_v2.0.a1 Prupe.6G233700_v2.0.a1 Prupe.6G233700_v2.0.a1
pyrus_communis	pycom04g08940 pycom04g08950 pycom09g14580 pycom11g11820 pycom12g11360
rosa_chinensis	RchiOBHm_Chr3g0470061 RchiOBHm_Chr4g0421621 RchiOBHm_Chr6g0259281 RchiOBHm_Chr6g0259871
rosa_laevigata	RLG00000007663 RLG00000014503 RLG00000014543 RLG00000024300
rosa_multiflora	Rmu_co8522155.1_g000003 Rmu_sc0005600.1_g000001 Rmu_sc0009049.1_g000005 Rmu_sc0011974.1_g000005 Rmu_sc0033604.1_g000001 Rmu_sc0033605.1_g000001 Rmu_ssc0000050.1_g000089
rosa_roxburghii	Rroxscaffold_5G00363870 Rroxscaffold_6G00411220
rosa_rugosa	Rorug03G0106600 Rorug04G0173900 Rorug05G0586800 Rorug05G0590900
rosa_samantha	Rh3AG155800 Rh4CG248200 Rh6AG103300 Rh6AG103400 Rh6AG107600 Rh6BG095700 Rh6CG092600 Rh6CG096400
rosa_wichuraiana	Rw3G014670 Rw4G020040 Rw6G008920 Rw6G009270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1519
AccB1I	GGYRCC	1 cut(s)	1018
AccII	CGCG	2 cut(s)	380, 1002
AciI	CCGC	4 cut(s)	482, 733, 1561, 1852
AclWI	GGATC	1 cut(s)	1793
AcoI	YGGCCR	1 cut(s)	1188
AcsI	RAATTY	2 cut(s)	1219, 1290
AcuI	CTGAAG	2 cut(s)	73, 1058
AcyI	GRCGYC	1 cut(s)	1516
AdeI	CACNNNGTG	1 cut(s)	1804
AfaI	GTAC	1 cut(s)	35
AfiI	CCNNNNNNNGG	8 cut(s)	81, 320, 349, 350, 1142, 1143, 1172, 1552
AgsI	TTSAA	5 cut(s)	29, 277, 866, 1408, 1576
AhlI	ACTAGT	1 cut(s)	1253
AjnI	CCWGG	4 cut(s)	126, 343, 1165, 1510
AleI	CACNNNNGTG	1 cut(s)	817
Alw21I	GWGCWC	3 cut(s)	851, 960, 1627
Alw26I	GTCTC	5 cut(s)	380, 803, 905, 1073, 1599
Alw44I	GTGCAC	1 cut(s)	847
AlwI	GGATC	1 cut(s)	1793
AlwNI	CAGNNNCTG	1 cut(s)	1877
Ama87I	CYCGRG	2 cut(s)	349, 369
AoxI	GGCC	9 cut(s)	338, 393, 416, 650, 726, 1159, 1188, 1238, 1467
ApaI	GGGCCC	1 cut(s)	342
ApaLI	GTGCAC	1 cut(s)	847
ApeKI	GCWGC	6 cut(s)	839, 857, 1388, 1391, 1793, 1880
ApoI	RAATTY	2 cut(s)	1219, 1290
AseI	ATTAAT	1 cut(s)	411
Asp700I	GAANNNNTTC	1 cut(s)	470
AspLEI	GCGC	1 cut(s)	862
AspS9I	GGNCC	8 cut(s)	338, 339, 393, 416, 1160, 1238, 1467, 1522
AsuC2I	CCSGG	1 cut(s)	730
AsuHPI	GGTGA	3 cut(s)	182, 788, 1125
AsuII	TTCGAA	1 cut(s)	748
AvaI	CYCGRG	2 cut(s)	349, 369
AvaII	GGWCC	1 cut(s)	1522
BaeGI	GKGCMC	2 cut(s)	342, 851
BalI	TGGCCA	1 cut(s)	1190
BanI	GGYRCC	1 cut(s)	1018
BanII	GRGCYC	2 cut(s)	342, 1627
BbsI	GAAGAC	2 cut(s)	194, 222
Bbv12I	GWGCWC	3 cut(s)	851, 960, 1627
BbvI	GCAGC	5 cut(s)	826, 844, 1375, 1378, 1805
BccI	CCATC	5 cut(s)	107, 235, 383, 596, 1424
BceAI	ACGGC	2 cut(s)	1023, 1806
BcgI	CGANNNNNNTGC	2 cut(s)	1334, 1368
BciT130I	CCWGG	4 cut(s)	128, 345, 1167, 1512
BciVI	GTATCC	1 cut(s)	1101
BcnI	CCSGG	1 cut(s)	730
BcoDI	GTCTC	5 cut(s)	380, 803, 905, 1073, 1599
BcuI	ACTAGT	1 cut(s)	1253
BfaI	CTAG	4 cut(s)	1254, 1305, 1641, 1888
BfmI	CTRYAG	1 cut(s)	1878
BfuI	GTATCC	1 cut(s)	1101
BisI	GCNGC	7 cut(s)	734, 840, 858, 1389, 1392, 1794, 1881
BlsI	GCNGC	7 cut(s)	735, 841, 859, 1390, 1393, 1795, 1882
Bme1390I	CCNGG	5 cut(s)	128, 345, 730, 1167, 1512
Bme18I	GGWCC	1 cut(s)	1522
BmeT110I	CYCGRG	2 cut(s)	349, 369
BmgT120I	GGNCC	8 cut(s)	338, 339, 393, 416, 1160, 1238, 1467, 1522
BmiI	GGNNCC	7 cut(s)	106, 340, 341, 394, 1020, 1239, 1468
BmrFI	CCNGG	5 cut(s)	128, 345, 730, 1167, 1512
BmrI	ACTGGG	1 cut(s)	907
BmsI	GCATC	4 cut(s)	910, 918, 1027, 1111
BmuI	ACTGGG	1 cut(s)	907
BpiI	GAAGAC	2 cut(s)	194, 222
BplI	GAGNNNNNCTC	2 cut(s)	1579, 1611
BpmI	CTGGAG	3 cut(s)	1073, 1398, 1581
Bpu10I	CCTNAGC	1 cut(s)	804
Bpu14I	TTCGAA	1 cut(s)	748
BpuEI	CTTGAG	2 cut(s)	758, 1606
BpuMI	CCSGG	1 cut(s)	730
BsaBI	GATNNNNATC	1 cut(s)	1535
BsaHI	GRCGYC	1 cut(s)	1516
BsaJI	CCNNGG	4 cut(s)	100, 343, 1166, 1191
BsaXI	ACNNNNNCTCC	4 cut(s)	413, 443, 517, 547
Bsc4I	CCNNNNNNNGG	8 cut(s)	81, 320, 349, 350, 1142, 1143, 1172, 1552
Bse1I	ACTGG	3 cut(s)	902, 1094, 1598
Bse3DI	GCAATG	3 cut(s)	100, 144, 1350
Bse8I	GATNNNNATC	1 cut(s)	1535
BseBI	CCWGG	4 cut(s)	128, 345, 1167, 1512
BseDI	CCNNGG	4 cut(s)	100, 343, 1166, 1191
BseGI	GGATG	8 cut(s)	607, 787, 892, 1042, 1102, 1435, 1536, 1777
BseJI	GATNNNNATC	1 cut(s)	1535
BseLI	CCNNNNNNNGG	8 cut(s)	81, 320, 349, 350, 1142, 1143, 1172, 1552
BseMI	GCAATG	3 cut(s)	100, 144, 1350
BseMII	CTCAG	7 cut(s)	194, 260, 843, 915, 1098, 1233, 1373
BseNI	ACTGG	3 cut(s)	902, 1094, 1598
BseRI	GAGGAG	2 cut(s)	583, 1781
BseSI	GKGCMC	2 cut(s)	342, 851
BseXI	GCAGC	5 cut(s)	826, 844, 1375, 1378, 1805
BseYI	CCCAGC	2 cut(s)	440, 667
BsgI	GTGCAG	1 cut(s)	1482
Bsh1236I	CGCG	2 cut(s)	380, 1002
BshFI	GGCC	9 cut(s)	340, 395, 418, 652, 728, 1161, 1190, 1240, 1469
BshNI	GGYRCC	1 cut(s)	1018
BsiHKAI	GWGCWC	3 cut(s)	851, 960, 1627
BsiHKCI	CYCGRG	2 cut(s)	349, 369
BsiSI	CCGG	2 cut(s)	419, 729
BslFI	GGGAC	1 cut(s)	98
BslI	CCNNNNNNNGG	8 cut(s)	81, 320, 349, 350, 1142, 1143, 1172, 1552
BsmAI	GTCTC	5 cut(s)	380, 803, 905, 1073, 1599
BsmBI	CGTCTC	1 cut(s)	380
BsmFI	GGGAC	1 cut(s)	98
BsmI	GAATGC	1 cut(s)	496
BsnI	GGCC	9 cut(s)	340, 395, 418, 652, 728, 1161, 1190, 1240, 1469
BsoBI	CYCGRG	2 cut(s)	349, 369
Bsp119I	TTCGAA	1 cut(s)	748
Bsp120I	GGGCCC	1 cut(s)	338
Bsp1286I	GDGCHC	4 cut(s)	342, 851, 960, 1627
Bsp1407I	TGTACA	1 cut(s)	33
Bsp143I	GATC	4 cut(s)	49, 423, 904, 1785
Bsp68I	TCGCGA	2 cut(s)	380, 1002
BspACI	CCGC	4 cut(s)	482, 733, 1561, 1852
BspANI	GGCC	9 cut(s)	340, 395, 418, 652, 728, 1161, 1190, 1240, 1469
BspCNI	CTCAG	7 cut(s)	193, 259, 844, 916, 1099, 1234, 1372
BspFNI	CGCG	2 cut(s)	380, 1002
BspLI	GGNNCC	7 cut(s)	106, 340, 341, 394, 1020, 1239, 1468
BspMAI	CTGCAG	1 cut(s)	1882
BspPI	GGATC	1 cut(s)	1793
BspQI	GCTCTTC	1 cut(s)	1002
BspT104I	TTCGAA	1 cut(s)	748
BspT107I	GGYRCC	1 cut(s)	1018
BsrDI	GCAATG	3 cut(s)	100, 144, 1350
BsrGI	TGTACA	1 cut(s)	33
BsrI	ACTGG	3 cut(s)	902, 1094, 1598
BssECI	CCNNGG	4 cut(s)	100, 343, 1166, 1191
BssMI	GATC	4 cut(s)	49, 423, 904, 1785
BssNI	GRCGYC	1 cut(s)	1516
BssT1I	CCWWGG	1 cut(s)	1191
Bst2UI	CCWGG	4 cut(s)	128, 345, 1167, 1512
Bst4CI	ACNGT	3 cut(s)	104, 498, 1453
Bst6I	CTCTTC	4 cut(s)	533, 542, 1002, 1058
BstACI	GRCGYC	1 cut(s)	1516
BstAPI	GCANNNNNTGC	1 cut(s)	1877
BstAUI	TGTACA	1 cut(s)	33
BstBI	TTCGAA	1 cut(s)	748
BstC8I	GCNNGC	6 cut(s)	978, 982, 1582, 1679, 1683, 1687
BstDSI	CCRYGG	1 cut(s)	100
BstF5I	GGATG	8 cut(s)	607, 787, 892, 1042, 1102, 1435, 1536, 1777
BstFNI	CGCG	2 cut(s)	380, 1002
BstHHI	GCGC	1 cut(s)	862
BstKTI	GATC	4 cut(s)	52, 426, 907, 1788
BstMAI	GTCTC	5 cut(s)	380, 803, 905, 1073, 1599
BstMBI	GATC	4 cut(s)	49, 423, 904, 1785
BstMWI	GCNNNNNNNGC	5 cut(s)	742, 836, 977, 1246, 1877
BstNI	CCWGG	4 cut(s)	128, 345, 1167, 1512
BstSCI	CCNGG	5 cut(s)	126, 343, 728, 1165, 1510
BstSFI	CTRYAG	1 cut(s)	1878
BstSLI	GKGCMC	2 cut(s)	342, 851
BstUI	CGCG	2 cut(s)	380, 1002
BstV1I	GCAGC	5 cut(s)	826, 844, 1375, 1378, 1805
BstV2I	GAAGAC	2 cut(s)	194, 222
BsuI	GTATCC	1 cut(s)	1101
BsuRI	GGCC	9 cut(s)	340, 395, 418, 652, 728, 1161, 1190, 1240, 1469
BtgI	CCRYGG	1 cut(s)	100
BtgZI	GCGATG	1 cut(s)	361
BtsCI	GGATG	8 cut(s)	607, 787, 892, 1042, 1102, 1435, 1536, 1777
BtsI	GCAGTG	1 cut(s)	1875
BtsIMutI	CAGTG	2 cut(s)	849, 1875
BtuMI	TCGCGA	2 cut(s)	380, 1002
Cac8I	GCNNGC	6 cut(s)	978, 982, 1582, 1679, 1683, 1687
CaiI	CAGNNNCTG	1 cut(s)	1877
CfoI	GCGC	1 cut(s)	862
Cfr13I	GGNCC	8 cut(s)	338, 339, 393, 416, 1160, 1238, 1467, 1522
CseI	GACGC	1 cut(s)	128
Csp6I	GTAC	1 cut(s)	34
CviAII	CATG	4 cut(s)	427, 598, 766, 815
CviQI	GTAC	1 cut(s)	34
DpnI	GATC	4 cut(s)	51, 425, 906, 1787
DpnII	GATC	4 cut(s)	49, 423, 904, 1785
DraIII	CACNNNGTG	1 cut(s)	1804
EaeI	YGGCCR	1 cut(s)	1188
Eam1104I	CTCTTC	4 cut(s)	533, 542, 1002, 1058
EarI	CTCTTC	4 cut(s)	533, 542, 1002, 1058
Ecl136II	GAGCTC	1 cut(s)	1625
Eco130I	CCWWGG	1 cut(s)	1191
Eco24I	GRGCYC	2 cut(s)	342, 1627
Eco32I	GATATC	3 cut(s)	1663, 1723, 1849
Eco47I	GGWCC	1 cut(s)	1522
Eco53kI	GAGCTC	1 cut(s)	1625
Eco57I	CTGAAG	2 cut(s)	73, 1058
Eco88I	CYCGRG	2 cut(s)	349, 369
EcoICRI	GAGCTC	1 cut(s)	1625
EcoO109I	RGGNCCY	3 cut(s)	339, 393, 1238
EcoRII	CCWGG	4 cut(s)	126, 343, 1165, 1510
EcoRV	GATATC	3 cut(s)	1663, 1723, 1849
EcoT14I	CCWWGG	1 cut(s)	1191
EcoT22I	ATGCAT	1 cut(s)	872
EcoT38I	GRGCYC	2 cut(s)	342, 1627
ErhI	CCWWGG	1 cut(s)	1191
Esp3I	CGTCTC	1 cut(s)	380
FaeI	CATG	4 cut(s)	430, 601, 769, 818
FalI	AAGNNNNNCTT	6 cut(s)	71, 103, 973, 1005, 1568, 1600
FaqI	GGGAC	1 cut(s)	98
FatI	CATG	4 cut(s)	426, 597, 765, 814
FauI	CCCGC	2 cut(s)	475, 1568
FauNDI	CATATG	1 cut(s)	1867
Fnu4HI	GCNGC	7 cut(s)	734, 840, 858, 1389, 1392, 1794, 1881
FokI	GGATG	8 cut(s)	614, 794, 899, 1049, 1089, 1442, 1523, 1784
FriOI	GRGCYC	2 cut(s)	342, 1627
Fsp4HI	GCNGC	7 cut(s)	734, 840, 858, 1389, 1392, 1794, 1881
FspBI	CTAG	4 cut(s)	1254, 1305, 1641, 1888
GlaI	GCGC	1 cut(s)	861
GluI	GCNGC	7 cut(s)	734, 840, 858, 1389, 1392, 1794, 1881
GsaI	CCCAGC	2 cut(s)	444, 671
GsuI	CTGGAG	3 cut(s)	1073, 1398, 1581
HaeIII	GGCC	9 cut(s)	340, 395, 418, 652, 728, 1161, 1190, 1240, 1469
HapII	CCGG	2 cut(s)	419, 729
HgaI	GACGC	1 cut(s)	128
HhaI	GCGC	1 cut(s)	862
Hin1I	GRCGYC	1 cut(s)	1516
Hin1II	CATG	4 cut(s)	430, 601, 769, 818
Hin6I	GCGC	1 cut(s)	860
HinP1I	GCGC	1 cut(s)	860
HindIII	AAGCTT	1 cut(s)	770
HinfI	GANTC	9 cut(s)	147, 173, 250, 358, 750, 1346, 1556, 1808, 1841
HpaII	CCGG	2 cut(s)	419, 729
HphI	GGTGA	3 cut(s)	182, 788, 1125
Hpy166II	GTNNAC	3 cut(s)	100, 849, 1615
Hpy8I	GTNNAC	3 cut(s)	100, 849, 1615
Hpy99I	CGWCG	1 cut(s)	122
HpyAV	CCTTC	3 cut(s)	193, 460, 999
HpyCH4III	ACNGT	3 cut(s)	104, 498, 1453
HpyCH4IV	ACGT	2 cut(s)	219, 1516
HpyF10VI	GCNNNNNNNGC	5 cut(s)	742, 836, 977, 1246, 1877
HpySE526I	ACGT	2 cut(s)	219, 1516
Hsp92I	GRCGYC	1 cut(s)	1516
Hsp92II	CATG	4 cut(s)	430, 601, 769, 818
HspAI	GCGC	1 cut(s)	860
Kzo9I	GATC	4 cut(s)	49, 423, 904, 1785
LguI	GCTCTTC	1 cut(s)	1002
LmnI	GCTCC	3 cut(s)	265, 538, 1321
Lsp1109I	GCAGC	5 cut(s)	826, 844, 1375, 1378, 1805
LweI	GCATC	4 cut(s)	910, 918, 1027, 1111
MaeI	CTAG	4 cut(s)	1254, 1305, 1641, 1888
MaeII	ACGT	2 cut(s)	219, 1516
MaeIII	GTNAC	3 cut(s)	170, 1027, 1131
MalI	GATC	4 cut(s)	51, 425, 906, 1787
MboI	GATC	4 cut(s)	49, 423, 904, 1785
MfeI	CAATTG	1 cut(s)	1526
MhlI	GDGCHC	4 cut(s)	342, 851, 960, 1627
MlsI	TGGCCA	1 cut(s)	1190
MluCI	AATT	8 cut(s)	41, 61, 204, 891, 1219, 1290, 1526, 1750
MluNI	TGGCCA	1 cut(s)	1190
MlyI	GAGTC	3 cut(s)	167, 352, 1817
MmeI	TCCRAC	2 cut(s)	771, 928
Mox20I	TGGCCA	1 cut(s)	1190
Mph1103I	ATGCAT	1 cut(s)	872
MroXI	GAANNNNTTC	1 cut(s)	470
MscI	TGGCCA	1 cut(s)	1190
MseI	TTAA	2 cut(s)	294, 411
MslI	CAYNNNNRTG	3 cut(s)	305, 817, 819
Msp20I	TGGCCA	1 cut(s)	1190
MspA1I	CMGCKG	1 cut(s)	1563
MspI	CCGG	2 cut(s)	419, 729
MspR9I	CCNGG	5 cut(s)	128, 345, 730, 1167, 1512
MunI	CAATTG	1 cut(s)	1526
Mva1269I	GAATGC	1 cut(s)	496
MvaI	CCWGG	4 cut(s)	128, 345, 1167, 1512
MvnI	CGCG	2 cut(s)	380, 1002
MwoI	GCNNNNNNNGC	5 cut(s)	742, 836, 977, 1246, 1877
NciI	CCSGG	1 cut(s)	730
NdeI	CATATG	1 cut(s)	1867
NdeII	GATC	4 cut(s)	49, 423, 904, 1785
NlaIII	CATG	4 cut(s)	430, 601, 769, 818
NlaIV	GGNNCC	7 cut(s)	106, 340, 341, 394, 1020, 1239, 1468
NmuCI	GTSAC	3 cut(s)	170, 1027, 1131
NruI	TCGCGA	2 cut(s)	380, 1002
NsiI	ATGCAT	1 cut(s)	872
NspV	TTCGAA	1 cut(s)	748
OliI	CACNNNNGTG	1 cut(s)	817
PciSI	GCTCTTC	1 cut(s)	1002
PcsI	WCGNNNNNNNCGW	1 cut(s)	533
PctI	GAATGC	1 cut(s)	496
PdmI	GAANNNNTTC	1 cut(s)	470
PfeI	GAWTC	6 cut(s)	147, 250, 750, 1346, 1556, 1841
PfoI	TCCNGGA	1 cut(s)	1510
PkrI	GCNGC	7 cut(s)	735, 841, 859, 1390, 1393, 1795, 1882
PleI	GAGTC	3 cut(s)	167, 352, 1816
PpsI	GAGTC	3 cut(s)	167, 352, 1816
PshBI	ATTAAT	1 cut(s)	411
Psp124BI	GAGCTC	1 cut(s)	1627
Psp6I	CCWGG	4 cut(s)	126, 343, 1165, 1510
PspFI	CCCAGC	2 cut(s)	440, 667
PspGI	CCWGG	4 cut(s)	126, 343, 1165, 1510
PspN4I	GGNNCC	7 cut(s)	106, 340, 341, 394, 1020, 1239, 1468
PspOMI	GGGCCC	1 cut(s)	338
PspPI	GGNCC	8 cut(s)	338, 339, 393, 416, 1160, 1238, 1467, 1522
PstI	CTGCAG	1 cut(s)	1882
PstNI	CAGNNNCTG	1 cut(s)	1877
RruI	TCGCGA	2 cut(s)	380, 1002
RsaI	GTAC	1 cut(s)	35
RsaNI	GTAC	1 cut(s)	34
RseI	CAYNNNNRTG	3 cut(s)	305, 817, 819
SacI	GAGCTC	1 cut(s)	1627
SapI	GCTCTTC	1 cut(s)	1002
SaqAI	TTAA	2 cut(s)	294, 411
SatI	GCNGC	7 cut(s)	734, 840, 858, 1389, 1392, 1794, 1881
Sau3AI	GATC	4 cut(s)	49, 423, 904, 1785
Sau96I	GGNCC	8 cut(s)	338, 339, 393, 416, 1160, 1238, 1467, 1522
SchI	GAGTC	3 cut(s)	167, 352, 1817
ScrFI	CCNGG	5 cut(s)	128, 345, 730, 1167, 1512
SduI	GDGCHC	4 cut(s)	342, 851, 960, 1627
SfaNI	GCATC	4 cut(s)	910, 918, 1027, 1111
SfcI	CTRYAG	1 cut(s)	1878
SfuI	TTCGAA	1 cut(s)	748
SinI	GGWCC	1 cut(s)	1522
SmiMI	CAYNNNNRTG	3 cut(s)	305, 817, 819
SmlI	CTYRAG	2 cut(s)	737, 1585
SmoI	CTYRAG	2 cut(s)	737, 1585
SpeI	ACTAGT	1 cut(s)	1253
Sse9I	AATT	8 cut(s)	41, 61, 204, 891, 1219, 1290, 1526, 1750
SsiI	CCGC	4 cut(s)	482, 733, 1561, 1852
SspI	AATATT	1 cut(s)	1572
SspMI	CTAG	4 cut(s)	1254, 1305, 1641, 1888
SstI	GAGCTC	1 cut(s)	1627
StyD4I	CCNGG	5 cut(s)	126, 343, 728, 1165, 1510
StyI	CCWWGG	1 cut(s)	1191
TaaI	ACNGT	3 cut(s)	104, 498, 1453
TaiI	ACGT	2 cut(s)	222, 1519
TaqI	TCGA	6 cut(s)	431, 474, 748, 1296, 1344, 1811
TasI	AATT	8 cut(s)	41, 61, 204, 891, 1219, 1290, 1526, 1750
TatI	WGTACW	1 cut(s)	33
TauI	GCSGC	1 cut(s)	736
TfiI	GAWTC	6 cut(s)	147, 250, 750, 1346, 1556, 1841
Tru1I	TTAA	2 cut(s)	294, 411
Tru9I	TTAA	2 cut(s)	294, 411
TscAI	CASTG	2 cut(s)	856, 1882
TseFI	GTSAC	3 cut(s)	170, 1027, 1131
TseI	GCWGC	6 cut(s)	839, 857, 1388, 1391, 1793, 1880
Tsp45I	GTSAC	3 cut(s)	170, 1027, 1131
TspGWI	ACGGA	1 cut(s)	1496
TspRI	CASTG	2 cut(s)	856, 1882
VneI	GTGCAC	1 cut(s)	847
VpaK11BI	GGWCC	1 cut(s)	1522
VspI	ATTAAT	1 cut(s)	411
XapI	RAATTY	2 cut(s)	1219, 1290
XmnI	GAANNNNTTC	1 cut(s)	470
XspI	CTAG	4 cut(s)	1254, 1305, 1641, 1888
ZraI	GACGTC	1 cut(s)	1517
Zsp2I	ATGCAT	1 cut(s)	872

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.