RchiOBHm_Chr6g0259871

Cell Division

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001165
Physical Location & Seq
Forward (+)
15045406 .. 15047808
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23303

Sequence Viewer

Length: 2403 bp
ATGACGAACCAACGTGAAGGAACAAATAAGCGGGACTTCAGCACCGCGATTCTGGACCGCAAGAAGGCTCCAAATCGCCTTGTTGTCGATGAAGCAGTGGTGGATGACAACTCCACCATCCAACTCTGCCCGGAGACCATGGAGAAGCTCCAGCTCTTTCGTGGCGACACAGTCTTGATCAAGGGAAAGAAGCATAGAGACACAATCTGTATTGCTCTTGCTGATGACACCTGTGAAGAACCAAAGATAAGGATGAACAAGGTTGTGAGGAACAACCTTAGGGTTAGGCTTGGTGATGTTGTTTCCATTCACCAATGCGCTGATGTCAAGTATGGAAACCGTGTCCACATTCTTCCTATAGATGACACAATCGAAGGGATCACTGGAAATCTGTTTGATGCCTACTTGAAGCCTTATTTCCAGGAAGCCTACCGCCCGGTGAGGAAAGGCGATCTTTTCCTAGTGAGAGGAGGAATGAGGAGTGTGGAGTTCAAGGTTGTTGAGACTGATCCTCCTGAGTATTGTGTGGTTGCTTCAGACACTGAAATTTTCTGCGAGGGAGAACCTGTTAGAAGGGACGATGAGGAAAGATTAGATGAGGTAGGGTATGATGATATTGGTGGAGTTAGGAAACAGATGGCTCAGATTCGCGAATTGGTTGAGCTGCCTCTGAGGCATCCTCAGCTGTTTAAATCAATAGGTGTCAAACCACCAAAGGGTATTCTGCTCTATGGTCCTCCTGGAACTGGAAAGACTCTAATTGCAAGAGCTGTTGCTAATGAAACTGGGGCCTTCTTTTTCTGTATCAATGGACCAGAAATCATGTCCAAGATGGCTGGAGAGAGTGAGAGCAACCTCAGGAAAGCTTTTGAGGAAGCAGAGAAGAATGCACCATCAATCATATTTATTGATGAGATTGATTCCATTGCTCCCAAGAGAGAGAAGACTAATGGAGAAATTGAGAGGAGGATTGTTTCCCAGCTCTTGACACTTATGGATGGACTAAAGTCCCGAGCACATGTAATTGTAATGGCGGCTACTAATCGTCCAAACAGTATTGACCCGGCTCTTAGAAGGTTTGGAAGATTTGATAGGGAAATAGATATTGGTGTTCCAGATGAAGTTGGGCGCCTTGAGGTCCTCCACATTCATACCAAGAACATGAAGCTTGCTGAAGATGTCGATTTAGAAAGGGTTGCCAAGGACACTCACGGTTATGTTGGGGCTGATCTAGCTGCTTTATCAACTGAGGCTGCACTTCAATGCATCCGAGAAAAGATGGATGTGATTGACTTGGAAGATGAGACAATAGATGCTGAGGTACTTAACTCAATGGCGGTTACAAATGAGCACTTCCAAACTGCTCTTGGAACAAGCAACCCATCTGCTCTTCGTGAGACGGTTGTTGAAGTGCCCAATGTCACTTGGGATGCAATTGGAGGTCTGGAAAATGTTAAGAGAGAACTTCAAGAGACTGTTCAATATCCTGTTGAGCACCCGGAGAAATTTGAGAAATTCGGAATGTCACCCTCAAAGGGAGTTCTTTTCTATGGCCCTCCTGGATGCGGTAAAACTCTTCTGGCCAAGGCTATTGCCAATGAGTGTCAGGCAAACTTCATCAGTATCAAGGGACCTGAATTGCTAACCATGTGGTTTGGAGAGAGTGAGGCCAATGTCAGGGAAATATTTGACAAGGCTCGTGCATCGGCTCCATGTGTCCTCTTCTTCGATGAGCTTGATTCCATTGCTACTAAAAGAGGTAGTAGTGCAGGTGATTCTGGAGGTGCTGATAGGGTTTTGAACCAACTCCTTACAGAAATGGATGGAGTGTCTGCCAAGAAAACTGTGTTCATTATTGGGGCAACCAACAGACCTGACATTATAGACCCTGCACTTCTTCGACCAGGACGTCTGGATCAGTTGCTTTACATCCCTCTCCCTGATGAGGAATCTCGCTATCAAATATTCAAGTCTTGCTTGAGGAAATCTCCGGTTTCCAAATATGTGGACCTCAGTGCTCTTGCCAAGCACACACCAGGATTTAGTGGTGCTGATATCACAGAAATATGCCAGCGTGCTTGCAAATATGCCATTAGAGACAACATTGAGAAAGATATCGAGAGGGAGAGGAGGAGAAGTGAGAATCCTGAGATTATGGAAGATGATGAGAATGAAGTAGCTGAGATTACTGCAGCTCATTTTGAGGAATCGATGAAGTATGCTCGCAGGAGCGTCAGTGATGCTGATATCCTCAAGTACCAGGCATTTGCGACTACATTGCACCAGGCAAGAGGGTTTGGAGCAGATGAGTTCAGGTTTCCCAGTGGTTCGGGTGCTGCTATTGGATCTGATCCTTTTGCAACTACTAACGCTGGTGAAGCTGATGAAGATCTTTATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
AAA CDC48_2 CDC48_N

Protein Analysis

800

Amino Acids

89.01

Weight (kDa)

5.08

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_N PF02359 26 - 107 3.8e-21 Cell division protein 48 (CDC48), N-terminal domain
CDC48_2 PF02933 127 - 191 5.9e-12 Cell division protein 48 (CDC48), domain 2
RuvB_N PF05496 240 - 314 8.9e-06 Holliday junction DNA helicase RuvB P-loop domain
AAA PF00004 241 - 370 2.7e-48 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 393 - 434 4.1e-14 AAA+ lid domain
AAA PF00004 514 - 646 3.2e-47 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 669 - 707 1.6e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1760
AasI GACNNNNNNGTC 1 cut(s) 1908
AatII GACGTC 1 cut(s) 1912
Acc36I ACCTGC 1 cut(s) 1760
AccB1I GGYRCC 1 cut(s) 1128
AccII CGCG 2 cut(s) 47, 651
AciI CCGC 7 cut(s) 31, 45, 58, 433, 1034, 1337, 1566
AclWI GGATC 5 cut(s) 386, 503, 1923, 2345, 2353
AcoI YGGCCR 1 cut(s) 1581
AcsI RAATTY 3 cut(s) 546, 1505, 1514
AcuI CTGAAG 3 cut(s) 22, 519, 1194
AcyI GRCGYC 2 cut(s) 1129, 1909
AfaI GTAC 2 cut(s) 1323, 2258
AfiI CCNNNNNNNGG 7 cut(s) 64, 716, 746, 1535, 1565, 1677, 1945
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 8 cut(s) 409, 493, 1262, 1409, 1469, 1481, 1801, 1969
AjnI CCWGG 7 cut(s) 420, 739, 1558, 1903, 2035, 2259, 2283
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
Alw21I GWGCWC 4 cut(s) 1018, 1353, 1497, 2020
Alw26I GTCTC 7 cut(s) 128, 192, 497, 1298, 1391, 1466, 2091
AlwI GGATC 5 cut(s) 386, 503, 1923, 2345, 2353
AlwNI CAGNNNCTG 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 1011
AoxI GGCC 4 cut(s) 789, 1552, 1581, 1668
ApeKI GCWGC 5 cut(s) 664, 1235, 1253, 2192, 2336
ApoI RAATTY 3 cut(s) 546, 1505, 1514
AspLEI GCGC 2 cut(s) 320, 1131
AspS9I GGNCC 8 cut(s) 55, 734, 789, 812, 1138, 1553, 1631, 2008
AsuC2I CCSGG 4 cut(s) 131, 437, 1064, 1499
AsuHPI GGTGA 6 cut(s) 302, 305, 451, 1518, 1784, 2387
AvaI CYCGRG 1 cut(s) 1011
AvaII GGWCC 6 cut(s) 55, 734, 812, 1138, 1631, 2008
AxyI CCTNAGG 2 cut(s) 278, 857
BaeGI GKGCMC 1 cut(s) 1416
BalI TGGCCA 1 cut(s) 1583
BanI GGYRCC 1 cut(s) 1128
BauI CACGAG 1 cut(s) 1698
BbsI GAAGAC 1 cut(s) 950
Bbv12I GWGCWC 4 cut(s) 1018, 1353, 1497, 2020
BbvCI CCTCAGC 2 cut(s) 681, 1317
BbvI GCAGC 5 cut(s) 651, 1222, 1240, 2204, 2323
BccI CCATC 8 cut(s) 125, 631, 826, 901, 992, 1273, 1390, 1817
BcgI CGANNNNNNTGC 2 cut(s) 2260, 2294
BciT130I CCWGG 7 cut(s) 422, 741, 1560, 1905, 2037, 2261, 2285
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 4 cut(s) 131, 437, 1064, 1499
BcoDI GTCTC 7 cut(s) 128, 192, 497, 1298, 1391, 1466, 2091
BfaI CTAG 2 cut(s) 461, 1232
BfmI CTRYAG 2 cut(s) 357, 2190
BfoI RGCGCY 1 cut(s) 1132
BfuAI ACCTGC 1 cut(s) 1760
BglI GCCNNNNNGGC 1 cut(s) 673
BglII AGATCT 1 cut(s) 2389
BisI GCNGC 6 cut(s) 665, 1035, 1236, 1254, 2193, 2337
BlsI GCNGC 6 cut(s) 666, 1036, 1237, 1255, 2194, 2338
Bme18I GGWCC 6 cut(s) 55, 734, 812, 1138, 1631, 2008
BmeT110I CYCGRG 1 cut(s) 1011
BmgT120I GGNCC 8 cut(s) 55, 734, 789, 812, 1138, 1553, 1631, 2008
BmiI GGNNCC 5 cut(s) 69, 790, 1130, 1632, 1710
BmrI ACTGGG 2 cut(s) 795, 2316
BmsI GCATC 8 cut(s) 388, 685, 1275, 1303, 1420, 1553, 1712, 2230
BmuI ACTGGG 2 cut(s) 795, 2316
BoxI GACNNNNGTC 1 cut(s) 1006
BpiI GAAGAC 1 cut(s) 950
BplI GAGNNNNNCTC 4 cut(s) 664, 696, 1972, 2004
BpmI CTGGAG 3 cut(s) 134, 858, 1800
Bpu10I CCTNAGC 2 cut(s) 681, 1317
BpuEI CTTGAG 3 cut(s) 1154, 1999, 2237
BpuMI CCSGG 4 cut(s) 131, 437, 1064, 1499
Bsa29I ATCGAT 1 cut(s) 2210
BsaBI GATNNNNATC 1 cut(s) 2388
BsaHI GRCGYC 2 cut(s) 1129, 1909
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 138, 1200, 1584
BsaWI WCCGGW 1 cut(s) 1990
BsaXI ACNNNNNCTCC 8 cut(s) 496, 526, 552, 582, 2122, 2152, 2221, 2251
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 716, 746, 1535, 1565, 1677, 1945
Bse1I ACTGG 4 cut(s) 388, 751, 790, 2322
Bse21I CCTNAGG 2 cut(s) 278, 857
Bse3DI GCAATG 3 cut(s) 924, 1743, 2276
Bse8I GATNNNNATC 1 cut(s) 2388
BseBI CCWGG 7 cut(s) 422, 741, 1560, 1905, 2037, 2261, 2285
BseCI ATCGAT 1 cut(s) 2210
BseDI CCNNGG 3 cut(s) 138, 1200, 1584
BseJI GATNNNNATC 1 cut(s) 2388
BseLI CCNNNNNNNGG 7 cut(s) 64, 716, 746, 1535, 1565, 1677, 1945
BseMI GCAATG 3 cut(s) 924, 1743, 2276
BseNI ACTGG 4 cut(s) 388, 751, 790, 2322
BseRI GAGGAG 5 cut(s) 483, 493, 979, 2143, 2146
BseSI GKGCMC 1 cut(s) 1416
BseXI GCAGC 5 cut(s) 651, 1222, 1240, 2204, 2323
BseYI CCCAGC 1 cut(s) 978
BsgI GTGCAG 3 cut(s) 1239, 1788, 1875
Bsh1236I CGCG 2 cut(s) 47, 651
BshFI GGCC 4 cut(s) 791, 1554, 1583, 1670
BshNI GGYRCC 1 cut(s) 1128
BshVI ATCGAT 1 cut(s) 2210
BsiHKAI GWGCWC 4 cut(s) 1018, 1353, 1497, 2020
BsiHKCI CYCGRG 1 cut(s) 1011
BsiSI CCGG 5 cut(s) 131, 437, 1064, 1499, 1991
BslFI GGGAC 4 cut(s) 47, 590, 994, 1644
BslI CCNNNNNNNGG 7 cut(s) 64, 716, 746, 1535, 1565, 1677, 1945
BsmAI GTCTC 7 cut(s) 128, 192, 497, 1298, 1391, 1466, 2091
BsmBI CGTCTC 1 cut(s) 1391
BsmFI GGGAC 4 cut(s) 47, 590, 994, 1644
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 4 cut(s) 791, 1554, 1583, 1670
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 1011
Bsp1286I GDGCHC 5 cut(s) 1018, 1353, 1416, 1497, 2020
Bsp143I GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
Bsp19I CCATGG 1 cut(s) 138
Bsp68I TCGCGA 1 cut(s) 651
BspACI CCGC 7 cut(s) 31, 45, 58, 433, 1034, 1337, 1566
BspANI GGCC 4 cut(s) 791, 1554, 1583, 1670
BspDI ATCGAT 1 cut(s) 2210
BspFNI CGCG 2 cut(s) 47, 651
BspLI GGNNCC 5 cut(s) 69, 790, 1130, 1632, 1710
BspMAI CTGCAG 1 cut(s) 2194
BspMI ACCTGC 1 cut(s) 1760
BspPI GGATC 5 cut(s) 386, 503, 1923, 2345, 2353
BspQI GCTCTTC 1 cut(s) 1395
BspT107I GGYRCC 1 cut(s) 1128
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 3 cut(s) 924, 1743, 2276
BsrI ACTGG 4 cut(s) 388, 751, 790, 2322
BssECI CCNNGG 3 cut(s) 138, 1200, 1584
BssMI GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
BssNI GRCGYC 2 cut(s) 1129, 1909
BssSI CACGAG 1 cut(s) 1698
BssT1I CCWWGG 3 cut(s) 138, 1200, 1584
Bst2BI CACGAG 1 cut(s) 1698
Bst2UI CCWGG 7 cut(s) 422, 741, 1560, 1905, 2037, 2261, 2285
Bst4CI ACNGT 7 cut(s) 172, 341, 1055, 1214, 1402, 1477, 1846
Bst6I CTCTTC 3 cut(s) 1395, 1581, 1727
BstACI GRCGYC 2 cut(s) 1129, 1909
BstC8I GCNNGC 5 cut(s) 1170, 2072, 2076, 2080, 2224
BstDSI CCRYGG 1 cut(s) 138
BstFNI CGCG 2 cut(s) 47, 651
BstH2I RGCGCY 1 cut(s) 1132
BstHHI GCGC 2 cut(s) 320, 1131
BstKTI GATC 9 cut(s) 180, 381, 454, 511, 1231, 1918, 2348, 2353, 2392
BstMAI GTCTC 7 cut(s) 128, 192, 497, 1298, 1391, 1466, 2091
BstMBI GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
BstMWI GCNNNNNNNGC 4 cut(s) 673, 682, 1232, 2378
BstNI CCWGG 7 cut(s) 422, 741, 1560, 1905, 2037, 2261, 2285
BstNSI RCATGY 1 cut(s) 1022
BstPAI GACNNNNGTC 1 cut(s) 1006
BstSFI CTRYAG 2 cut(s) 357, 2190
BstSLI GKGCMC 1 cut(s) 1416
BstUI CGCG 2 cut(s) 47, 651
BstV1I GCAGC 5 cut(s) 651, 1222, 1240, 2204, 2323
BstV2I GAAGAC 1 cut(s) 950
BstX2I RGATCY 2 cut(s) 2345, 2389
BstXI CCANNNNNNTGG 1 cut(s) 2005
BstYI RGATCY 2 cut(s) 2345, 2389
Bsu15I ATCGAT 1 cut(s) 2210
Bsu36I CCTNAGG 2 cut(s) 278, 857
BsuRI GGCC 4 cut(s) 791, 1554, 1583, 1670
BsuTUI ATCGAT 1 cut(s) 2210
BtgI CCRYGG 1 cut(s) 138
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 6 cut(s) 102, 381, 540, 2020, 2242, 2329
BtuMI TCGCGA 1 cut(s) 651
BveI ACCTGC 1 cut(s) 1760
Cac8I GCNNGC 5 cut(s) 1170, 2072, 2076, 2080, 2224
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 2 cut(s) 320, 1131
Cfr13I GGNCC 8 cut(s) 55, 734, 789, 812, 1138, 1553, 1631, 2008
ClaI ATCGAT 1 cut(s) 2210
CseI GACGC 1 cut(s) 2221
Csp6I GTAC 2 cut(s) 1322, 2257
CviAII CATG 6 cut(s) 139, 823, 1019, 1162, 1648, 1713
CviQI GTAC 2 cut(s) 1322, 2257
DinI GGCGCC 1 cut(s) 1130
DpnI GATC 9 cut(s) 179, 380, 453, 510, 1230, 1917, 2347, 2352, 2391
DpnII GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
DraI TTTAAA 1 cut(s) 691
DrdI GACNNNNNNGTC 1 cut(s) 1908
DseDI GACNNNNNNGTC 1 cut(s) 1908
EaeI YGGCCR 1 cut(s) 1581
Eam1104I CTCTTC 3 cut(s) 1395, 1581, 1727
EarI CTCTTC 3 cut(s) 1395, 1581, 1727
Eco130I CCWWGG 3 cut(s) 138, 1200, 1584
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 3 cut(s) 2056, 2116, 2248
Eco47I GGWCC 6 cut(s) 55, 734, 812, 1138, 1631, 2008
Eco57I CTGAAG 3 cut(s) 22, 519, 1194
Eco81I CCTNAGG 2 cut(s) 278, 857
Eco88I CYCGRG 1 cut(s) 1011
EcoO109I RGGNCCY 3 cut(s) 789, 1138, 1631
EcoRII CCWGG 7 cut(s) 420, 739, 1558, 1903, 2035, 2259, 2283
EcoRV GATATC 3 cut(s) 2056, 2116, 2248
EcoT14I CCWWGG 3 cut(s) 138, 1200, 1584
EcoT22I ATGCAT 1 cut(s) 1268
EgeI GGCGCC 1 cut(s) 1130
EheI GGCGCC 1 cut(s) 1130
ErhI CCWWGG 3 cut(s) 138, 1200, 1584
Esp3I CGTCTC 1 cut(s) 1391
FaeI CATG 6 cut(s) 142, 826, 1022, 1165, 1651, 1716
FalI AAGNNNNNCTT 6 cut(s) 20, 52, 438, 470, 1961, 1993
FaqI GGGAC 4 cut(s) 47, 590, 994, 1644
FatI CATG 6 cut(s) 138, 822, 1018, 1161, 1647, 1712
FauI CCCGC 1 cut(s) 24
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 6 cut(s) 665, 1035, 1236, 1254, 2193, 2337
Fsp4HI GCNGC 6 cut(s) 665, 1035, 1236, 1254, 2193, 2337
FspBI CTAG 2 cut(s) 461, 1232
GlaI GCGC 2 cut(s) 319, 1130
GluI GCNGC 6 cut(s) 665, 1035, 1236, 1254, 2193, 2337
GsaI CCCAGC 1 cut(s) 982
GsuI CTGGAG 3 cut(s) 134, 858, 1800
HaeII RGCGCY 1 cut(s) 1132
HaeIII GGCC 4 cut(s) 791, 1554, 1583, 1670
HapII CCGG 5 cut(s) 131, 437, 1064, 1499, 1991
HgaI GACGC 1 cut(s) 2221
HhaI GCGC 2 cut(s) 320, 1131
Hin1I GRCGYC 2 cut(s) 1129, 1909
Hin1II CATG 6 cut(s) 142, 826, 1022, 1165, 1651, 1716
Hin6I GCGC 2 cut(s) 318, 1129
HinP1I GCGC 2 cut(s) 318, 1129
HindIII AAGCTT 2 cut(s) 864, 1166
HinfI GANTC 9 cut(s) 49, 646, 754, 920, 1739, 1775, 1949, 2143, 2207
HpaII CCGG 5 cut(s) 131, 437, 1064, 1499, 1991
HphI GGTGA 6 cut(s) 302, 305, 451, 1518, 1784, 2387
Hpy166II GTNNAC 2 cut(s) 346, 2008
Hpy188I TCNGA 6 cut(s) 538, 645, 672, 1271, 1520, 2350
Hpy8I GTNNAC 2 cut(s) 346, 2008
HpyAV CCTTC 6 cut(s) 11, 58, 368, 567, 802, 1068
HpyCH4III ACNGT 7 cut(s) 172, 341, 1055, 1214, 1402, 1477, 1846
HpyCH4IV ACGT 2 cut(s) 13, 1909
HpyF10VI GCNNNNNNNGC 4 cut(s) 673, 682, 1232, 2378
HpySE526I ACGT 2 cut(s) 13, 1909
Hsp92I GRCGYC 2 cut(s) 1129, 1909
Hsp92II CATG 6 cut(s) 142, 826, 1022, 1165, 1651, 1716
HspAI GCGC 2 cut(s) 318, 1129
KasI GGCGCC 1 cut(s) 1128
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
LguI GCTCTTC 1 cut(s) 1395
LmnI GCTCC 6 cut(s) 73, 153, 934, 1714, 2229, 2300
Lsp1109I GCAGC 5 cut(s) 651, 1222, 1240, 2204, 2323
LweI GCATC 8 cut(s) 388, 685, 1275, 1303, 1420, 1553, 1712, 2230
MaeI CTAG 2 cut(s) 461, 1232
MaeII ACGT 2 cut(s) 13, 1909
MaeIII GTNAC 3 cut(s) 1339, 1420, 1524
MalI GATC 9 cut(s) 179, 380, 453, 510, 1230, 1917, 2347, 2352, 2391
MboI GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 2 cut(s) 2345, 2389
MhlI GDGCHC 5 cut(s) 1018, 1353, 1416, 1497, 2020
MlsI TGGCCA 1 cut(s) 1583
MluCI AATT 9 cut(s) 546, 653, 759, 957, 1023, 1434, 1505, 1514, 1637
MluNI TGGCCA 1 cut(s) 1583
Mly113I GGCGCC 1 cut(s) 1129
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 1 cut(s) 145
Mox20I TGGCCA 1 cut(s) 1583
Mph1103I ATGCAT 1 cut(s) 1268
MscI TGGCCA 1 cut(s) 1583
MseI TTAA 3 cut(s) 690, 1326, 1455
MslI CAYNNNNRTG 2 cut(s) 1215, 1261
Msp20I TGGCCA 1 cut(s) 1583
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 5 cut(s) 131, 437, 1064, 1499, 1991
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 7 cut(s) 422, 741, 1560, 1905, 2037, 2261, 2285
MvnI CGCG 2 cut(s) 47, 651
MwoI GCNNNNNNNGC 4 cut(s) 673, 682, 1232, 2378
NarI GGCGCC 1 cut(s) 1129
NciI CCSGG 4 cut(s) 131, 437, 1064, 1499
NcoI CCATGG 1 cut(s) 138
NdeII GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
NlaIII CATG 6 cut(s) 142, 826, 1022, 1165, 1651, 1716
NlaIV GGNNCC 5 cut(s) 69, 790, 1130, 1632, 1710
NmuCI GTSAC 2 cut(s) 1420, 1524
NruI TCGCGA 1 cut(s) 651
NsiI ATGCAT 1 cut(s) 1268
NspI RCATGY 1 cut(s) 1022
PaqCI CACCTGC 1 cut(s) 1760
PciI ACATGT 1 cut(s) 1018
PciSI GCTCTTC 1 cut(s) 1395
PcsI WCGNNNNNNNCGW 1 cut(s) 1906
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 8 cut(s) 49, 646, 920, 1739, 1775, 1949, 2143, 2207
PflFI GACNNNGTC 1 cut(s) 170
PfoI TCCNGGA 3 cut(s) 420, 739, 1558
PkrI GCNGC 6 cut(s) 666, 1036, 1237, 1255, 2194, 2338
PleI GAGTC 1 cut(s) 748
PluTI GGCGCC 1 cut(s) 1132
PpsI GAGTC 1 cut(s) 748
PpuMI RGGWCCY 2 cut(s) 1138, 1631
PscI ACATGT 1 cut(s) 1018
PshAI GACNNNNGTC 1 cut(s) 1006
Psp5II RGGWCCY 2 cut(s) 1138, 1631
Psp6I CCWGG 7 cut(s) 420, 739, 1558, 1903, 2035, 2259, 2283
PspFI CCCAGC 1 cut(s) 978
PspGI CCWGG 7 cut(s) 420, 739, 1558, 1903, 2035, 2259, 2283
PspN4I GGNNCC 5 cut(s) 69, 790, 1130, 1632, 1710
PspPI GGNCC 8 cut(s) 55, 734, 789, 812, 1138, 1553, 1631, 2008
PspPPI RGGWCCY 2 cut(s) 1138, 1631
PstI CTGCAG 1 cut(s) 2194
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 2 cut(s) 2345, 2389
PsyI GACNNNGTC 1 cut(s) 170
PvuII CAGCTG 1 cut(s) 685
RruI TCGCGA 1 cut(s) 651
RsaI GTAC 2 cut(s) 1323, 2258
RsaNI GTAC 2 cut(s) 1322, 2257
RseI CAYNNNNRTG 2 cut(s) 1215, 1261
SapI GCTCTTC 1 cut(s) 1395
SaqAI TTAA 3 cut(s) 690, 1326, 1455
SatI GCNGC 6 cut(s) 665, 1035, 1236, 1254, 2193, 2337
Sau3AI GATC 9 cut(s) 177, 378, 451, 508, 1228, 1915, 2345, 2350, 2389
Sau96I GGNCC 8 cut(s) 55, 734, 789, 812, 1138, 1553, 1631, 2008
SchI GAGTC 1 cut(s) 748
SduI GDGCHC 5 cut(s) 1018, 1353, 1416, 1497, 2020
SfaNI GCATC 8 cut(s) 388, 685, 1275, 1303, 1420, 1553, 1712, 2230
SfcI CTRYAG 2 cut(s) 357, 2190
SfoI GGCGCC 1 cut(s) 1130
SinI GGWCC 6 cut(s) 55, 734, 812, 1138, 1631, 2008
SmiMI CAYNNNNRTG 2 cut(s) 1215, 1261
SmlI CTYRAG 3 cut(s) 1133, 1978, 2252
SmoI CTYRAG 3 cut(s) 1133, 1978, 2252
Sse9I AATT 9 cut(s) 546, 653, 759, 957, 1023, 1434, 1505, 1514, 1637
SsiI CCGC 7 cut(s) 31, 45, 58, 433, 1034, 1337, 1566
SspDI GGCGCC 1 cut(s) 1128
SspI AATATT 2 cut(s) 1686, 1965
SspMI CTAG 2 cut(s) 461, 1232
StyI CCWWGG 3 cut(s) 138, 1200, 1584
TaaI ACNGT 7 cut(s) 172, 341, 1055, 1214, 1402, 1477, 1846
TaiI ACGT 2 cut(s) 16, 1912
TaqI TCGA 7 cut(s) 87, 372, 1182, 1728, 1900, 2118, 2210
TasI AATT 9 cut(s) 546, 653, 759, 957, 1023, 1434, 1505, 1514, 1637
TauI GCSGC 1 cut(s) 1037
TfiI GAWTC 8 cut(s) 49, 646, 920, 1739, 1775, 1949, 2143, 2207
Tru1I TTAA 3 cut(s) 690, 1326, 1455
Tru9I TTAA 3 cut(s) 690, 1326, 1455
TscAI CASTG 6 cut(s) 102, 388, 547, 2020, 2242, 2329
TseFI GTSAC 2 cut(s) 1420, 1524
TseI GCWGC 5 cut(s) 664, 1235, 1253, 2192, 2336
Tsp45I GTSAC 2 cut(s) 1420, 1524
TspRI CASTG 6 cut(s) 102, 388, 547, 2020, 2242, 2329
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 6 cut(s) 55, 734, 812, 1138, 1631, 2008
XapI RAATTY 3 cut(s) 546, 1505, 1514
XceI RCATGY 1 cut(s) 1022
XcmI CCANNNNNNNNNTGG 2 cut(s) 158, 1364
XspI CTAG 2 cut(s) 461, 1232
ZraI GACGTC 1 cut(s) 1910
Zsp2I ATGCAT 1 cut(s) 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.