Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000719

Physical Location & Seq

Genomic Coordinates Reverse (-)

3000612 .. 3003539

Gene Structure 2928 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G09690.12

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGTCACCTGACGGAGAACTTGTTCCGGTGGATTCATCGGCGGTAGTCACGGCGAAGAGGAAGACATCTCAGTTATCAAGGAGTTGGATTTCCATAGACGCCACGGGGCAAAAAACTGTGCTTGACGTTGACAAACACGTGATTATGCACCGTGTTCAAATCCACGCTCGTGATCTCCGGATCCTTGACCCGAATCTATTTTACCCTTCTGCTATTTTGGGTCGAGAAAGAGCCATTGTTCTTAACTTAGAGCATATCAAGGCGATTATCACTGCCGAAGAGGTTTTGATCCGAGATTCGTCAGATGAAAATGTTATCCCCGTCCTGGAGGAGTTTCAGAGAAGATTACCTGTAGGAAACGAGGCACATGGTGTTCACGGAGATGGTGATTTGGGTGAAGAAGATGAATCCCCGTTTGAATTCCGGGCGCTAGAGGTGGCCTTGGAAGCTATTTGTAGTTTTCTAGCTGCAAGGACAACAGAACTAGAGAAGTTTGCTTATCCTGCTTTGGATGAACTTACCTTGAAGATAAGTAGCCGTAACTTGGAAAGAGTTCGTAAATTGAAGAGTGCCATGACCCGTTTGACAGCTCGGGTCCAAAAGGTAAGGGATGAGCTTGAACAGTTGTTGGATGATGATGGTGATATGGCTGATCTCTACCTTACAAGGAAGCTTGTTGGTGCATCTTCATCTGTCAGCGTTTCAGATGAACCCATTTGGTATCCTACTTCCCCAACAATAGGTTCTATGATTTCCAGAGCAAGTAGAGTGAGTTTAGTCACGGTTCGTGGAGACGACGAAACTGATGTTGAAGAGCTTGAAATGTTGCTTGAGGCATACTTCATGCAAATAGACAGCACTTTGAACAAATTAACCGAGCTACGCGAGTATATTGATGACACAGAGGATTACATTAACATCCAGTTAGACAATCATCGAAATCAGCTGATTCAGTTAGAGCTAATGTTAAGCGCAGGAACCGTCTGTGTATCAGTGTACTCTATGATCGCCGGAATATTCGGCATGAACATTCCCAACACATGGAACCACGACCATGGTTACATTTTCAAATGGGTCGTAAGTCTCACGGGAACATTTTGCATAGTCTTGTTCGTGATTATACTGTCGTACGCTCGGTTTAGAGGACTCATCGGATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0009555 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0032501 GO:0032502 GO:0034220 GO:0044464 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1903830

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

386

Amino Acids

43.43

Weight (kDa)

4.86

Isoelectric Point (pI)

37.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRS2-like	PF22099	45 - 235	5.7e-28	Magnesium transporter MRS2-like
MRS2-like	PF22099	274 - 324	7.1e-08	Magnesium transporter MRS2-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000719)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09690 AT5G09720 AT5G09720 AT5G64560 AT5G64560
fragaria_vesca	FvH4_2g22370 FvH4_2g22370 FvH4_2g22410 FvH4_4g02330 FvH4_4g02330
malus_domestica	MD05G1204800.v1.1 MD10G1191900.v1.1 MD13G1205200.v1.1 MD16G1207000.v1.1
prunus_persica	Prupe.1G027800_v2.0.a1 Prupe.8G231400_v2.0.a1 Prupe.8G231400_v2.0.a1 Prupe.8G231400_v2.0.a1 Prupe.8G231400_v2.0.a1 Prupe.8G231400_v2.0.a1
pyrus_communis	pycom05g19100 pycom10g16560 pycom10g16580 pycom13g17790 pycom16g17380
rosa_chinensis	RchiOBHm_Chr2g0150571 RchiOBHm_Chr4g0389661 RchiOBHm_Chr4g0389711 RchiOBHm_Chr6g0289361
rosa_laevigata	RLG00000009961 RLG00000012309
rosa_multiflora	Rmu_sc0001527.1_g000030 Rmu_sc0005115.1_g000007 Rmu_sc0005697.1_g000020 Rmu_sc0007419.1_g000012
rosa_roxburghii	Rroxscaffold_5G00335660 Rroxscaffold_5G00335700 Rroxscaffold_7G00179320
rosa_rugosa	Rorug03G0310000 Rorug03G0324700 Rorug04G0112800 Rorug04G0112900 Rorug04G0113000
rosa_samantha	Rh4AG029400 Rh4AG029700 Rh4BG022600 Rh4BG022800 Rh4CG031300 Rh4DG023000 Rh5DG350100 Rh6BG321400 Rh6CG327700 Rh6DG313300
rosa_wichuraiana	Rw3G027490 Rw4G002190 Rw4G002220 Rw4G006140 Rw6G003570 Rw6G005340 Rw6G027140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1041
AccII	CGCG	1 cut(s)	885
AccIII	TCCGGA	1 cut(s)	177
AciI	CCGC	1 cut(s)	41
AclWI	GGATC	3 cut(s)	175, 188, 283
AcsI	RAATTY	1 cut(s)	419
AcvI	CACGTG	1 cut(s)	139
AcyI	GRCGYC	1 cut(s)	99
AdeI	CACNNNGTG	1 cut(s)	371
AfaI	GTAC	2 cut(s)	998, 1130
AfiI	CCNNNNNNNGG	4 cut(s)	325, 544, 740, 1041
AflIII	ACRYGT	1 cut(s)	136
AgsI	TTSAA	9 cut(s)	158, 419, 526, 565, 620, 812, 821, 865, 1069
AjnI	CCWGG	1 cut(s)	324
AjuI	GAANNNNNNNTTGG	2 cut(s)	727, 759
AleI	CACNNNNGTG	1 cut(s)	168
AloI	GAACNNNNNNTCC	1 cut(s)	38
AluBI	AGCT	9 cut(s)	449, 467, 590, 616, 673, 817, 880, 946, 961
AluI	AGCT	9 cut(s)	449, 467, 590, 616, 673, 817, 880, 946, 961
Alw26I	GTCTC	2 cut(s)	786, 1088
AlwI	GGATC	3 cut(s)	175, 188, 283
Ama87I	CYCGRG	1 cut(s)	591
Aor13HI	TCCGGA	1 cut(s)	177
AoxI	GGCC	1 cut(s)	438
ApeKI	GCWGC	1 cut(s)	467
ApoI	RAATTY	1 cut(s)	419
Asp700I	GAANNNNTTC	2 cut(s)	21, 552
AspLEI	GCGC	2 cut(s)	430, 974
AspS9I	GGNCC	1 cut(s)	595
AsuC2I	CCSGG	1 cut(s)	425
AsuHPI	GGTGA	3 cut(s)	398, 407, 653
AvaI	CYCGRG	1 cut(s)	591
AvaII	GGWCC	1 cut(s)	595
BamHI	GGATCC	1 cut(s)	180
BauI	CACGAG	1 cut(s)	168
BbrPI	CACGTG	1 cut(s)	139
BbsI	GAAGAC	1 cut(s)	68
BbvI	GCAGC	1 cut(s)	454
BccI	CCATC	2 cut(s)	377, 632
BceAI	ACGGC	2 cut(s)	66, 522
BciT130I	CCWGG	1 cut(s)	326
BciVI	GTATCC	1 cut(s)	732
BcnI	CCSGG	1 cut(s)	425
BcoDI	GTCTC	2 cut(s)	786, 1088
BfaI	CTAG	3 cut(s)	431, 464, 485
BfmI	CTRYAG	1 cut(s)	351
BfoI	RGCGCY	1 cut(s)	431
BfuI	GTATCC	1 cut(s)	732
BisI	GCNGC	1 cut(s)	468
BlsI	GCNGC	1 cut(s)	469
Bme1390I	CCNGG	2 cut(s)	326, 425
Bme18I	GGWCC	1 cut(s)	595
BmeT110I	CYCGRG	1 cut(s)	591
BmgT120I	GGNCC	1 cut(s)	595
BmiI	GGNNCC	4 cut(s)	182, 596, 979, 1046
BmrFI	CCNGG	2 cut(s)	326, 425
BmsI	GCATC	1 cut(s)	692
BpiI	GAAGAC	1 cut(s)	68
BpmI	CTGGAG	1 cut(s)	347
BpuEI	CTTGAG	1 cut(s)	851
BpuMI	CCSGG	1 cut(s)	425
BsaAI	YACGTR	1 cut(s)	139
BsaHI	GRCGYC	1 cut(s)	99
BsaJI	CCNNGG	3 cut(s)	102, 441, 1054
BsaWI	WCCGGW	2 cut(s)	25, 177
BsaXI	ACNNNNNCTCC	2 cut(s)	6, 36
Bsc4I	CCNNNNNNNGG	4 cut(s)	325, 544, 740, 1041
Bse1I	ACTGG	1 cut(s)	922
BseAI	TCCGGA	1 cut(s)	177
BseBI	CCWGG	1 cut(s)	326
BseDI	CCNNGG	3 cut(s)	102, 441, 1054
BseGI	GGATG	4 cut(s)	517, 616, 637, 918
BseLI	CCNNNNNNNGG	4 cut(s)	325, 544, 740, 1041
BseMII	CTCAG	1 cut(s)	83
BseNI	ACTGG	1 cut(s)	922
BseRI	GAGGAG	1 cut(s)	344
BseXI	GCAGC	1 cut(s)	454
Bsh1236I	CGCG	1 cut(s)	885
BshFI	GGCC	1 cut(s)	440
BsiHKCI	CYCGRG	1 cut(s)	591
BsiSI	CCGG	4 cut(s)	26, 178, 424, 1011
BsiWI	CGTACG	1 cut(s)	1128
BslI	CCNNNNNNNGG	4 cut(s)	325, 544, 740, 1041
BsmAI	GTCTC	2 cut(s)	786, 1088
BsmBI	CGTCTC	1 cut(s)	786
BsnI	GGCC	1 cut(s)	440
BsoBI	CYCGRG	1 cut(s)	591
Bsp13I	TCCGGA	1 cut(s)	177
Bsp143I	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
Bsp19I	CCATGG	1 cut(s)	1054
BspACI	CCGC	1 cut(s)	41
BspANI	GGCC	1 cut(s)	440
BspCNI	CTCAG	1 cut(s)	82
BspEI	TCCGGA	1 cut(s)	177
BspFNI	CGCG	1 cut(s)	885
BspLI	GGNNCC	4 cut(s)	182, 596, 979, 1046
BspPI	GGATC	3 cut(s)	175, 188, 283
BspQI	GCTCTTC	1 cut(s)	807
BsrI	ACTGG	1 cut(s)	922
BssECI	CCNNGG	3 cut(s)	102, 441, 1054
BssMI	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
BssNI	GRCGYC	1 cut(s)	99
BssSI	CACGAG	1 cut(s)	168
BssT1I	CCWWGG	2 cut(s)	441, 1054
Bst2BI	CACGAG	1 cut(s)	168
Bst2UI	CCWGG	1 cut(s)	326
Bst4CI	ACNGT	6 cut(s)	118, 152, 624, 784, 982, 1125
Bst6I	CTCTTC	4 cut(s)	50, 273, 560, 807
BstACI	GRCGYC	1 cut(s)	99
BstBAI	YACGTR	1 cut(s)	139
BstDEI	CTNAG	2 cut(s)	69, 247
BstDSI	CCRYGG	2 cut(s)	102, 1054
BstF5I	GGATG	4 cut(s)	517, 616, 637, 918
BstFNI	CGCG	1 cut(s)	885
BstH2I	RGCGCY	1 cut(s)	431
BstHHI	GCGC	2 cut(s)	430, 974
BstKTI	GATC	6 cut(s)	175, 183, 291, 655, 1008, 1157
BstMAI	GTCTC	2 cut(s)	786, 1088
BstMBI	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
BstMWI	GCNNNNNNNGC	2 cut(s)	446, 503
BstNI	CCWGG	1 cut(s)	326
BstSCI	CCNGG	2 cut(s)	324, 423
BstSFI	CTRYAG	1 cut(s)	351
BstUI	CGCG	1 cut(s)	885
BstV1I	GCAGC	1 cut(s)	454
BstV2I	GAAGAC	1 cut(s)	68
BstX2I	RGATCY	2 cut(s)	180, 1154
BstXI	CCANNNNNNTGG	1 cut(s)	1055
BstYI	RGATCY	2 cut(s)	180, 1154
BsuI	GTATCC	1 cut(s)	732
BsuRI	GGCC	1 cut(s)	440
BtgI	CCRYGG	2 cut(s)	102, 1054
BtsCI	GGATG	4 cut(s)	517, 616, 637, 918
BtsI	GCAGTG	1 cut(s)	270
BtsIMutI	CAGTG	2 cut(s)	270, 999
CfoI	GCGC	2 cut(s)	430, 974
Cfr13I	GGNCC	1 cut(s)	595
CseI	GACGC	1 cut(s)	107
Csp6I	GTAC	2 cut(s)	997, 1129
CviAII	CATG	6 cut(s)	368, 574, 844, 1024, 1041, 1055
CviQI	GTAC	2 cut(s)	997, 1129
DdeI	CTNAG	2 cut(s)	69, 247
DpnI	GATC	6 cut(s)	174, 182, 290, 654, 1007, 1156
DpnII	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
DraIII	CACNNNGTG	1 cut(s)	371
Eam1104I	CTCTTC	4 cut(s)	50, 273, 560, 807
EarI	CTCTTC	4 cut(s)	50, 273, 560, 807
Eco130I	CCWWGG	2 cut(s)	441, 1054
Eco47I	GGWCC	1 cut(s)	595
Eco72I	CACGTG	1 cut(s)	139
Eco88I	CYCGRG	1 cut(s)	591
EcoRI	GAATTC	1 cut(s)	419
EcoRII	CCWGG	1 cut(s)	324
EcoT14I	CCWWGG	2 cut(s)	441, 1054
ErhI	CCWWGG	2 cut(s)	441, 1054
Esp3I	CGTCTC	1 cut(s)	786
FaeI	CATG	6 cut(s)	371, 577, 847, 1027, 1044, 1058
FatI	CATG	6 cut(s)	367, 573, 843, 1023, 1040, 1054
Fnu4HI	GCNGC	1 cut(s)	468
FokI	GGATG	4 cut(s)	524, 623, 644, 905
Fsp4HI	GCNGC	1 cut(s)	468
FspBI	CTAG	3 cut(s)	431, 464, 485
GlaI	GCGC	2 cut(s)	429, 973
GluI	GCNGC	1 cut(s)	468
GsuI	CTGGAG	1 cut(s)	347
HaeII	RGCGCY	1 cut(s)	431
HaeIII	GGCC	1 cut(s)	440
HapII	CCGG	4 cut(s)	26, 178, 424, 1011
HgaI	GACGC	1 cut(s)	107
HhaI	GCGC	2 cut(s)	430, 974
Hin1I	GRCGYC	1 cut(s)	99
Hin1II	CATG	6 cut(s)	371, 577, 847, 1027, 1044, 1058
Hin6I	GCGC	2 cut(s)	428, 972
HinP1I	GCGC	2 cut(s)	428, 972
HincII	GTYRAC	1 cut(s)	130
HindII	GTYRAC	1 cut(s)	130
HindIII	AAGCTT	1 cut(s)	671
HinfI	GANTC	6 cut(s)	32, 193, 296, 407, 949, 1146
HpaII	CCGG	4 cut(s)	26, 178, 424, 1011
HphI	GGTGA	3 cut(s)	398, 407, 653
Hpy166II	GTNNAC	3 cut(s)	130, 376, 997
Hpy188I	TCNGA	5 cut(s)	293, 304, 339, 706, 1154
Hpy188III	TCNNGA	5 cut(s)	170, 178, 224, 756, 1114
Hpy8I	GTNNAC	3 cut(s)	130, 376, 997
Hpy99I	CGWCG	1 cut(s)	800
HpyAV	CCTTC	1 cut(s)	216
HpyCH4III	ACNGT	6 cut(s)	118, 152, 624, 784, 982, 1125
HpyCH4IV	ACGT	2 cut(s)	126, 138
HpyCH4V	TGCA	5 cut(s)	148, 470, 683, 847, 1101
HpyF10VI	GCNNNNNNNGC	2 cut(s)	446, 503
HpyF3I	CTNAG	2 cut(s)	69, 247
HpySE526I	ACGT	2 cut(s)	126, 138
Hsp92I	GRCGYC	1 cut(s)	99
Hsp92II	CATG	6 cut(s)	371, 577, 847, 1027, 1044, 1058
HspAI	GCGC	2 cut(s)	428, 972
Kpn2I	TCCGGA	1 cut(s)	177
Kzo9I	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
LguI	GCTCTTC	1 cut(s)	807
Lsp1109I	GCAGC	1 cut(s)	454
LweI	GCATC	1 cut(s)	692
MaeI	CTAG	3 cut(s)	431, 464, 485
MaeII	ACGT	2 cut(s)	126, 138
MaeIII	GTNAC	5 cut(s)	3, 46, 539, 778, 1058
MalI	GATC	6 cut(s)	174, 182, 290, 654, 1007, 1156
MboI	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
MflI	RGATCY	2 cut(s)	180, 1154
MluCI	AATT	3 cut(s)	419, 560, 869
MlyI	GAGTC	1 cut(s)	1140
MmeI	TCCRAC	2 cut(s)	65, 609
MnlI	CCTC	8 cut(s)	51, 274, 322, 355, 427, 826, 898, 1136
MroI	TCCGGA	1 cut(s)	177
MroXI	GAANNNNTTC	2 cut(s)	21, 552
MseI	TTAA	5 cut(s)	243, 872, 915, 968, 1159
MslI	CAYNNNNRTG	3 cut(s)	168, 381, 1053
MspA1I	CMGCKG	1 cut(s)	946
MspI	CCGG	4 cut(s)	26, 178, 424, 1011
MspR9I	CCNGG	2 cut(s)	326, 425
MvaI	CCWGG	1 cut(s)	326
MvnI	CGCG	1 cut(s)	885
MwoI	GCNNNNNNNGC	2 cut(s)	446, 503
NciI	CCSGG	1 cut(s)	425
NcoI	CCATGG	1 cut(s)	1054
NdeII	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
NlaIII	CATG	6 cut(s)	371, 577, 847, 1027, 1044, 1058
NlaIV	GGNNCC	4 cut(s)	182, 596, 979, 1046
NmuCI	GTSAC	3 cut(s)	3, 46, 778
OliI	CACNNNNGTG	1 cut(s)	168
PciSI	GCTCTTC	1 cut(s)	807
PdmI	GAANNNNTTC	2 cut(s)	21, 552
PfeI	GAWTC	5 cut(s)	32, 193, 296, 407, 949
Pfl23II	CGTACG	1 cut(s)	1128
PflMI	CCANNNNNTGG	1 cut(s)	1041
PfoI	TCCNGGA	1 cut(s)	324
PkrI	GCNGC	1 cut(s)	469
PleI	GAGTC	1 cut(s)	1140
PmaCI	CACGTG	1 cut(s)	139
PmlI	CACGTG	1 cut(s)	139
PpsI	GAGTC	1 cut(s)	1140
Ppu21I	YACGTR	1 cut(s)	139
Psp6I	CCWGG	1 cut(s)	324
PspCI	CACGTG	1 cut(s)	139
PspGI	CCWGG	1 cut(s)	324
PspLI	CGTACG	1 cut(s)	1128
PspN4I	GGNNCC	4 cut(s)	182, 596, 979, 1046
PspPI	GGNCC	1 cut(s)	595
PsuI	RGATCY	2 cut(s)	180, 1154
PvuII	CAGCTG	1 cut(s)	946
RsaI	GTAC	2 cut(s)	998, 1130
RsaNI	GTAC	2 cut(s)	997, 1129
RseI	CAYNNNNRTG	3 cut(s)	168, 381, 1053
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	5 cut(s)	243, 872, 915, 968, 1159
SatI	GCNGC	1 cut(s)	468
Sau3AI	GATC	6 cut(s)	172, 180, 288, 652, 1005, 1154
Sau96I	GGNCC	1 cut(s)	595
SchI	GAGTC	1 cut(s)	1140
ScrFI	CCNGG	2 cut(s)	326, 425
SfaNI	GCATC	1 cut(s)	692
SfcI	CTRYAG	1 cut(s)	351
SinI	GGWCC	1 cut(s)	595
SmiMI	CAYNNNNRTG	3 cut(s)	168, 381, 1053
SmlI	CTYRAG	1 cut(s)	830
SmoI	CTYRAG	1 cut(s)	830
Sse9I	AATT	3 cut(s)	419, 560, 869
SsiI	CCGC	1 cut(s)	41
SspI	AATATT	1 cut(s)	1017
SspMI	CTAG	3 cut(s)	431, 464, 485
StyD4I	CCNGG	2 cut(s)	324, 423
StyI	CCWWGG	2 cut(s)	441, 1054
TaaI	ACNGT	6 cut(s)	118, 152, 624, 784, 982, 1125
TaiI	ACGT	2 cut(s)	129, 141
TaqI	TCGA	2 cut(s)	223, 937
TasI	AATT	3 cut(s)	419, 560, 869
TatI	WGTACW	1 cut(s)	996
TfiI	GAWTC	5 cut(s)	32, 193, 296, 407, 949
Tru1I	TTAA	5 cut(s)	243, 872, 915, 968, 1159
Tru9I	TTAA	5 cut(s)	243, 872, 915, 968, 1159
TscAI	CASTG	2 cut(s)	277, 999
TseFI	GTSAC	3 cut(s)	3, 46, 778
TseI	GCWGC	1 cut(s)	467
Tsp45I	GTSAC	3 cut(s)	3, 46, 778
TspDTI	ATGAA	8 cut(s)	24, 321, 420, 528, 678, 723, 832, 1040
TspGWI	ACGGA	2 cut(s)	27, 393
TspRI	CASTG	2 cut(s)	277, 999
Van91I	CCANNNNNTGG	1 cut(s)	1041
VpaK11BI	GGWCC	1 cut(s)	595
XapI	RAATTY	1 cut(s)	419
XmnI	GAANNNNTTC	2 cut(s)	21, 552
XspI	CTAG	3 cut(s)	431, 464, 485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.