Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001083

Physical Location & Seq

Genomic Coordinates Forward (+)

9497939 .. 9503173

Gene Structure 5235 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G27000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGGCGACGACATCGGAGATCAACAATGATCTTTCGTTTTCCGTTGTATCGATTGTTGAAGATGTTCTTCAACAACATAGTAGCCGATCAAGCGACGTCGGTTTGGTTTCCAGAAAAGTTGAAGAATCTTCTTTGAGAAGATATGAAGCTGCTGGGTGGCTTAGAGATATGATCGGAGTTTCCAATGGTAAAGATTTTCCAGGAGAGCCTTCTGAAGAAGAGTTTAGGCTTGGATTGCGAAGTGGGATCGTTCTTTGTAATGTTCTTAACAAAGTTAATCCTGGATCAGTCTCAAAGGTCGTTGAAGCGCCGGACGATGTTGCAGATGGAGCTGCACTTTCAGCTTTTCAATACTTTGAAAACATAAGGAATTTTTTAGTAGCTATAGAGGAAATGGGGTTGCCATCATTTGAAGCTTCAGATATGGAGAAGGGAGGCAAGTCTATAAGGATAGTGAACTGCATTCTTGCCCTTAAGTCTTATAGTGAGTGGAAACTGAAAGGTGAAAATGGTCCATGGAGGTATGGATCTAACATGAAACATAACTTTGGATCGAGAAAACTATTTCTGAGAAAAAGTTCAGAGCCATTTGTTAGTTCTATATCGAGAACTCAGTCGACGGATATGTTATCAACTGATCAACCTCTGAGTTCTGATGGAGATTCTAGATCCATAAATGGGCTTGTTCGGTCATTCATTGCAGACAGAAAGCATGAAGATATTCCAAATGTTGTAGAGTCTGTGTTGAACAAAGTTATGGAAGAAGTCCAGCAGCGTTTATCAATCCACAATGAAATGATGAAATCAAGTTCAAAGCCTATTCCAGAAGATGATTCATCCTGTGAGACAGTGGTCCGGTCTCAGCTGTGTGATGCAAGACAACATGAGGAAGCAGAAGAAAATAGTCCGCCCCAGGTTGTGGAAAAAAAGTTTCAAAGGACAAATTTTGAACATCACGAAGAACAAAAGATTCTTCTGAATCAACAAAAACACATCCAGGAGTTGAAGCAAACCTTATACACTACAAAAGCAGGAATGAAATTGCTGCAGATGAAGTATCAAGAAGATTTCTTTCATCTAGGCAAGCATTTAAATGGTTTAGCTTATGCGGCTACGGGATACAAAAGGGTTCTTGAAGAAAACCGTAAACTGTACAATCTAGTGCAGGATCTAAAAGGAAACATACGGGTGTACTGTCGAGTTAGGCCGTTCTTGCCTGGGCAAGAAAGTGGTGGTTTGAGTGCCGTGGAAGACATAGACGAAGGGACCATCACGATCAGAGTTCCATCAAAGTATGGGAAAGCAGGACAAAAACCATTTATGTTCAACAAAGTCTTTGGTCCTTCTGCAACACAAGAGGAAGTGTTTTCAGACATGCAGCCTTTGGTACGGTCGGTTCTCGATGGCTACAATGTCTGCATCTTCGCTTATGGCCAAACCGGGTCAGGGAAAACTTTTACCATGACAGGGCCTAAGGAACTGACTGAAGAGAGCCTAGGTGTGAACTACAGGGCACTGGCAGATCTGTTTCTTCTATCAAATCAAAGAAAAGACACGACCAGCTACGAAATCTCAGTTCAGATGTTGGAAATTTACAACGAGCAAGTCAGAGATCTACTTGCCCAAGATGGCCAGACTAAAAGGTTAGAGATCAGAAACAACTCTCACAATGGAATCAATGTACCGGAAGCAAGTCTAGTGCCTGTCTCATCGACGGATGACGTTATACAGCTAATGGATTTGGGACACATGAATCGTGCAGTGAGCTCTACAGCCATGAATGACAGAAGTAGTCGATCTCACAGTTGTGTAACTGTTCATGTTCAAGGCAGAGACCTCACATCTGGCTCTATCCTCCATGGTTCTATGCATCTGGTTGATCTTGCAGGAAGCGAGAGAGTGGACAAGTCTGAGGTGACTGGAGACAGACTGAAGGAGGCTCAACACATCAACAAGTCCCTCTCGGCTCTTGGAGATGTCATCTCCTCGCTTTCCCAGAAGACTTCTCATGTGCCTTACAGAAACAGTAAACTCACTCAGCTGCTGCAGGACTCCCTCGGAGGATCAGCCAAGACACTTATGTTTGTCCACATAAGTCCAGAACCTGACACTCTCGGAGAAACTATTAGTACTCTGAAGTTTGCTGAACGAGTGGGGAGTGTTGAGCTAGGCGCTGCTCGTGTGAACAAAGATAACTCAGAGGTTAAGGAGCTTAAAGAGCAGATTGCTAATCTTAAGATGGCTCTAGTGAGGAAAGGAAATGGTAATGATGTACAACCAACAGCTATACCAATCAACCGTGAGAGAATATCGAGAAGAAGATCACTCGAAACTCCTACTATTCGACCCAAATTACCTACCATGGGAAACACATCAAACAACAGTAGGCCCCAGATCATGGATCTAAGTGGACCAGAGGCTTTTAACGACAGTACTGCATCTTCAAGAAGACACAGCTTAGATATCCATGAGCTAATGAAATCTTCTTCTCCGGCTTGGCCGAGGCAGCCACTCAATGGAAAAGACGAGGACAGAGAATCTAAGTCGGGCGAGTGGATCGATAAGCATGAAGAATTAATTCAAAATCAGAATCCAAATTCTCCCGAGCAATTCTACCAGTCAATGGTCCCTCAACAACAATCACTATATGGTGGGAAACAAGATTTCGAAGTGCAGAGTATTACAGATAACGAATCTGATGAAGCCGCAACAAGCGATTGCTCAGATTCCGATTTGCTGTGGCGATTGAGCGTTCAAGTGAATGTTCCCAAAGTTTCTAATATCCAAAACTCAGCAAACCCCAAACCAAAGAAAATTCAGCCCAGAACTGCTAAACTCTCAGAAACTAGAAGTTTAATTCCATCGCTGATCCCAGCACCAAGCAAGAGACCTCCTAACACAGTAAATTCACAGCCGCAGCGACCAACGAGAGATGGAAAACGCAGACTGAGTTTAGGCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009987 GO:0015629 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031534 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043621 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0046907 GO:0051012 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

987

Amino Acids

110.01

Weight (kDa)

6.47

Isoelectric Point (pI)

50.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	48 - 162	3.5e-14	Calponin homology (CH) domain
Microtub_bd	PF16796	380 - 540	1.8e-42	Microtubule binding
Kinesin	PF00225	400 - 720	2.2e-111	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001083)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT5G27000
fragaria_vesca	FvH4_2g14960 FvH4_6g07070 FvH4_6g07070 FvH4_6g07070 FvH4_6g07070 FvH4_6g07070
malus_domestica	MD04G1196400.v1.1 MD12G1209400.v1.1
prunus_persica	Prupe.6G316600_v2.0.a1
pyrus_communis	pycom12g19670
rosa_chinensis	RchiOBHm_Chr1g0318271 RchiOBHm_Chr2g0102121 RchiOBHm_Chr2g0167941 RchiOBHm_Chr3g0456621 RchiOBHm_Chr3g0456641 RchiOBHm_Chr5g0067221
rosa_laevigata	RLG00000003185 RLG00000003285 RLG00000003859 RLG00000003890 RLG00000025287 RLG00000033013
rosa_multiflora	Rmu_sc0000133.1_g000037 Rmu_sc0000894.1_g000040 Rmu_sc0001563.1_g000026 Rmu_sc0007742.1_g000030 Rmu_sc0012002.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120300 Rroxscaffold_2G00132430 Rroxscaffold_5G00367570 Rroxscaffold_6G00422580 Rroxscaffold_7G00200730
rosa_rugosa	Rorug03G0014400 Rorug05G0465400 Rorug05G0465500
rosa_samantha	Rh1AG324900 Rh1CG231300 Rh2AG394600 Rh3AG075200 Rh3BG077100 Rh3CG076700 Rh3CG076800 Rh3DG078700 Rh5BG530400 Rh5CG554800 Rh6AG058800
rosa_wichuraiana	Rw3G006440 Rw4G008320 Rw5G014820 Rw7G027120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	99
AccB7I	CCANNNNNTGG	4 cut(s)	919, 2398, 2516, 2623
AccI	GTMKAC	1 cut(s)	617
AciI	CCGC	4 cut(s)	908, 1109, 2706, 2915
AcoI	YGGCCR	3 cut(s)	1432, 1630, 2498
AcsI	RAATTY	6 cut(s)	370, 943, 1590, 2596, 2814, 2905
AcuI	CTGAAG	5 cut(s)	234, 402, 1506, 1952, 2156
AcyI	GRCGYC	1 cut(s)	96
AfaI	GTAC	7 cut(s)	1154, 1193, 1389, 1683, 2131, 2274, 2434
AfiI	CCNNNNNNNGG	8 cut(s)	678, 919, 1446, 1467, 2363, 2398, 2516, 2623
AflII	CTTAAG	2 cut(s)	473, 2234
AjnI	CCWGG	5 cut(s)	199, 280, 912, 996, 1216
AleI	CACNNNNGTG	1 cut(s)	1806
Alw21I	GWGCWC	1 cut(s)	1769
Alw26I	GTCTC	7 cut(s)	295, 839, 864, 1711, 1827, 1917, 2881
AlwNI	CAGNNNCTG	2 cut(s)	2044, 2105
Ama87I	CYCGRG	1 cut(s)	2603
AoxI	GGCC	6 cut(s)	1205, 1432, 1469, 1630, 2387, 2498
ApoI	RAATTY	6 cut(s)	370, 943, 1590, 2596, 2814, 2905
AseI	ATTAAT	1 cut(s)	2576
Asp700I	GAANNNNTTC	4 cut(s)	63, 577, 720, 2577
AspA2I	CCTAGG	1 cut(s)	1495
AspLEI	GCGC	2 cut(s)	310, 2174
AspS9I	GGNCC	8 cut(s)	512, 853, 1266, 1340, 1469, 2388, 2411, 2626
AsuC2I	CCSGG	1 cut(s)	1441
AsuHPI	GGTGA	2 cut(s)	515, 1927
AsuII	TTCGAA	1 cut(s)	2667
AvaI	CYCGRG	1 cut(s)	2603
AvaII	GGWCC	6 cut(s)	512, 853, 1266, 1340, 2411, 2626
AvrII	CCTAGG	1 cut(s)	1495
AxyI	CCTNAGG	1 cut(s)	1473
BaeGI	GKGCMC	1 cut(s)	1516
BalI	TGGCCA	2 cut(s)	1434, 1632
BanII	GRGCYC	1 cut(s)	1769
BarI	GAAGNNNNNNTAC	2 cut(s)	2425, 2457
BauI	CACGAG	1 cut(s)	2178
BbsI	GAAGAC	3 cut(s)	1257, 2006, 2455
Bbv12I	GWGCWC	1 cut(s)	1769
BceAI	ACGGC	2 cut(s)	1192, 1229
BcgI	CGANNNNNNTGC	2 cut(s)	2417, 2451
BciT130I	CCWGG	5 cut(s)	201, 282, 914, 998, 1218
BciVI	GTATCC	1 cut(s)	1112
BclI	TGATCA	1 cut(s)	637
BcnI	CCSGG	1 cut(s)	1441
BcoDI	GTCTC	7 cut(s)	295, 839, 864, 1711, 1827, 1917, 2881
BfaI	CTAG	8 cut(s)	666, 1079, 1160, 1496, 1697, 2168, 2246, 2847
BfmI	CTRYAG	5 cut(s)	384, 1046, 1507, 1770, 2045
BfoI	RGCGCY	2 cut(s)	311, 2175
BfrI	CTTAAG	2 cut(s)	473, 2234
BfuI	GTATCC	1 cut(s)	1112
BglII	AGATCT	2 cut(s)	1522, 1612
BlnI	CCTAGG	1 cut(s)	1495
BmcAI	AGTACT	2 cut(s)	2131, 2434
Bme1390I	CCNGG	6 cut(s)	201, 282, 914, 998, 1218, 1441
Bme18I	GGWCC	6 cut(s)	512, 853, 1266, 1340, 2411, 2626
BmeT110I	CYCGRG	1 cut(s)	2603
BmgT120I	GGNCC	8 cut(s)	512, 853, 1266, 1340, 1469, 2388, 2411, 2626
BmiI	GGNNCC	3 cut(s)	1267, 2390, 2628
BmrFI	CCNGG	6 cut(s)	201, 282, 914, 998, 1218, 1441
BmsI	GCATC	4 cut(s)	862, 1428, 1879, 2447
BoxI	GACNNNNGTC	1 cut(s)	851
BpiI	GAAGAC	3 cut(s)	1257, 2006, 2455
BpmI	CTGGAG	1 cut(s)	1941
Bpu14I	TTCGAA	1 cut(s)	2667
BpuMI	CCSGG	1 cut(s)	1441
Bsa29I	ATCGAT	2 cut(s)	50, 2559
BsaHI	GRCGYC	1 cut(s)	96
BsaI	GGTCTC	3 cut(s)	864, 1827, 2881
BsaJI	CCNNGG	9 cut(s)	515, 912, 1217, 1245, 1495, 1858, 2056, 2361, 2501
BsaWI	WCCGGW	2 cut(s)	855, 1684
Bsc4I	CCNNNNNNNGG	8 cut(s)	678, 919, 1446, 1467, 2363, 2398, 2516, 2623
Bse1I	ACTGG	3 cut(s)	1521, 1924, 2617
Bse21I	CCTNAGG	1 cut(s)	1473
Bse3DI	GCAATG	1 cut(s)	696
BseBI	CCWGG	5 cut(s)	201, 282, 914, 998, 1218
BseCI	ATCGAT	2 cut(s)	50, 2559
BseDI	CCNNGG	9 cut(s)	515, 912, 1217, 1245, 1495, 1858, 2056, 2361, 2501
BseGI	GGATG	3 cut(s)	836, 993, 1723
BseLI	CCNNNNNNNGG	8 cut(s)	678, 919, 1446, 1467, 2363, 2398, 2516, 2623
BseMI	GCAATG	1 cut(s)	696
BseNI	ACTGG	3 cut(s)	1521, 1924, 2617
BseRI	GAGGAG	1 cut(s)	1975
BseSI	GKGCMC	1 cut(s)	1516
BseYI	CCCAGC	2 cut(s)	152, 2872
BsgI	GTGCAG	4 cut(s)	318, 1184, 1779, 2693
Bsh1285I	CGRYCG	1 cut(s)	1394
BshFI	GGCC	6 cut(s)	1207, 1434, 1471, 1632, 2389, 2500
BshVI	ATCGAT	2 cut(s)	50, 2559
BsiEI	CGRYCG	1 cut(s)	1394
BsiHKAI	GWGCWC	1 cut(s)	1769
BsiHKCI	CYCGRG	1 cut(s)	2603
BsiSI	CCGG	5 cut(s)	311, 856, 1440, 1685, 2492
BslFI	GGGAC	4 cut(s)	1279, 1758, 1942, 2612
BslI	CCNNNNNNNGG	8 cut(s)	678, 919, 1446, 1467, 2363, 2398, 2516, 2623
BsmAI	GTCTC	7 cut(s)	295, 839, 864, 1711, 1827, 1917, 2881
BsmFI	GGGAC	4 cut(s)	1279, 1758, 1942, 2612
BsmI	GAATGC	1 cut(s)	462
BsnI	GGCC	6 cut(s)	1207, 1434, 1471, 1632, 2389, 2500
Bso31I	GGTCTC	3 cut(s)	864, 1827, 2881
BsoBI	CYCGRG	1 cut(s)	2603
Bsp119I	TTCGAA	1 cut(s)	2667
Bsp1286I	GDGCHC	2 cut(s)	1516, 1769
Bsp1407I	TGTACA	2 cut(s)	1152, 2272
Bsp19I	CCATGG	3 cut(s)	515, 1858, 2361
BspACI	CCGC	4 cut(s)	908, 1109, 2706, 2915
BspANI	GGCC	6 cut(s)	1207, 1434, 1471, 1632, 2389, 2500
BspDI	ATCGAT	2 cut(s)	50, 2559
BspLI	GGNNCC	3 cut(s)	1267, 2390, 2628
BspMAI	CTGCAG	2 cut(s)	1050, 2049
BspT104I	TTCGAA	1 cut(s)	2667
BspTI	CTTAAG	2 cut(s)	473, 2234
BspTNI	GGTCTC	3 cut(s)	864, 1827, 2881
BsrDI	GCAATG	1 cut(s)	696
BsrGI	TGTACA	2 cut(s)	1152, 2272
BsrI	ACTGG	3 cut(s)	1521, 1924, 2617
BssECI	CCNNGG	9 cut(s)	515, 912, 1217, 1245, 1495, 1858, 2056, 2361, 2501
BssNI	GRCGYC	1 cut(s)	96
BssSI	CACGAG	1 cut(s)	2178
BssT1I	CCWWGG	4 cut(s)	515, 1495, 1858, 2361
Bst2BI	CACGAG	1 cut(s)	2178
Bst2UI	CCWGG	5 cut(s)	201, 282, 914, 998, 1218
Bst6I	CTCTTC	2 cut(s)	213, 1482
BstACI	GRCGYC	1 cut(s)	96
BstAFI	CTTAAG	2 cut(s)	473, 2234
BstAUI	TGTACA	2 cut(s)	1152, 2272
BstBI	TTCGAA	1 cut(s)	2667
BstC8I	GCNNGC	1 cut(s)	1085
BstDSI	CCRYGG	4 cut(s)	515, 1245, 1858, 2361
BstF5I	GGATG	3 cut(s)	836, 993, 1723
BstH2I	RGCGCY	2 cut(s)	311, 2175
BstHHI	GCGC	2 cut(s)	310, 2174
BstMAI	GTCTC	7 cut(s)	295, 839, 864, 1711, 1827, 1917, 2881
BstMCI	CGRYCG	1 cut(s)	1394
BstMWI	GCNNNNNNNGC	8 cut(s)	90, 235, 329, 341, 1109, 1213, 2218, 2506
BstNI	CCWGG	5 cut(s)	201, 282, 914, 998, 1218
BstNSI	RCATGY	1 cut(s)	1378
BstPAI	GACNNNNGTC	1 cut(s)	851
BstSCI	CCNGG	6 cut(s)	199, 280, 912, 996, 1216, 1439
BstSFI	CTRYAG	5 cut(s)	384, 1046, 1507, 1770, 2045
BstSLI	GKGCMC	1 cut(s)	1516
BstV2I	GAAGAC	3 cut(s)	1257, 2006, 2455
BstX2I	RGATCY	6 cut(s)	527, 668, 1168, 1522, 1612, 2401
BstYI	RGATCY	6 cut(s)	527, 668, 1168, 1522, 1612, 2401
Bsu15I	ATCGAT	2 cut(s)	50, 2559
Bsu36I	CCTNAGG	1 cut(s)	1473
BsuI	GTATCC	1 cut(s)	1112
BsuRI	GGCC	6 cut(s)	1207, 1434, 1471, 1632, 2389, 2500
BsuTUI	ATCGAT	2 cut(s)	50, 2559
BtgI	CCRYGG	4 cut(s)	515, 1245, 1858, 2361
BtgZI	GCGATG	1 cut(s)	2847
BtsCI	GGATG	3 cut(s)	836, 993, 1723
BtsI	GCAGTG	1 cut(s)	1767
BtsIMutI	CAGTG	3 cut(s)	855, 1514, 1767
Cac8I	GCNNGC	1 cut(s)	1085
CaiI	CAGNNNCTG	2 cut(s)	2044, 2105
CfoI	GCGC	2 cut(s)	310, 2174
Cfr13I	GGNCC	8 cut(s)	512, 853, 1266, 1340, 1469, 2388, 2411, 2626
ClaI	ATCGAT	2 cut(s)	50, 2559
Csp6I	GTAC	7 cut(s)	1153, 1192, 1388, 1682, 2130, 2273, 2433
CviQI	GTAC	7 cut(s)	1153, 1192, 1388, 1682, 2130, 2273, 2433
DraI	TTTAAA	1 cut(s)	1092
EaeI	YGGCCR	3 cut(s)	1432, 1630, 2498
Eam1104I	CTCTTC	2 cut(s)	213, 1482
EarI	CTCTTC	2 cut(s)	213, 1482
EciI	GGCGGA	1 cut(s)	897
Ecl136II	GAGCTC	1 cut(s)	1767
Eco130I	CCWWGG	4 cut(s)	515, 1495, 1858, 2361
Eco24I	GRGCYC	1 cut(s)	1769
Eco31I	GGTCTC	3 cut(s)	864, 1827, 2881
Eco32I	GATATC	1 cut(s)	2464
Eco47I	GGWCC	6 cut(s)	512, 853, 1266, 1340, 2411, 2626
Eco53kI	GAGCTC	1 cut(s)	1767
Eco57I	CTGAAG	5 cut(s)	234, 402, 1506, 1952, 2156
Eco81I	CCTNAGG	1 cut(s)	1473
Eco88I	CYCGRG	1 cut(s)	2603
EcoICRI	GAGCTC	1 cut(s)	1767
EcoO109I	RGGNCCY	2 cut(s)	1469, 2388
EcoRII	CCWGG	5 cut(s)	199, 280, 912, 996, 1216
EcoRV	GATATC	1 cut(s)	2464
EcoT14I	CCWWGG	4 cut(s)	515, 1495, 1858, 2361
EcoT22I	ATGCAT	1 cut(s)	1872
EcoT38I	GRGCYC	1 cut(s)	1769
ErhI	CCWWGG	4 cut(s)	515, 1495, 1858, 2361
FalI	AAGNNNNNCTT	4 cut(s)	51, 83, 998, 1030
FaqI	GGGAC	4 cut(s)	1279, 1758, 1942, 2612
FbaI	TGATCA	1 cut(s)	637
FblI	GTMKAC	1 cut(s)	617
FokI	GGATG	3 cut(s)	823, 980, 1730
FriOI	GRGCYC	1 cut(s)	1769
FspBI	CTAG	8 cut(s)	666, 1079, 1160, 1496, 1697, 2168, 2246, 2847
GlaI	GCGC	2 cut(s)	309, 2173
GsaI	CCCAGC	2 cut(s)	156, 2876
GsuI	CTGGAG	1 cut(s)	1941
HaeII	RGCGCY	2 cut(s)	311, 2175
HaeIII	GGCC	6 cut(s)	1207, 1434, 1471, 1632, 2389, 2500
HapII	CCGG	5 cut(s)	311, 856, 1440, 1685, 2492
HhaI	GCGC	2 cut(s)	310, 2174
Hin1I	GRCGYC	1 cut(s)	96
Hin6I	GCGC	2 cut(s)	308, 2172
HinP1I	GCGC	2 cut(s)	308, 2172
HincII	GTYRAC	1 cut(s)	618
HindII	GTYRAC	1 cut(s)	618
HindIII	AAGCTT	1 cut(s)	414
HpaII	CCGG	5 cut(s)	311, 856, 1440, 1685, 2492
HphI	GGTGA	2 cut(s)	515, 1927
Hpy99I	CGWCG	5 cut(s)	10, 98, 101, 622, 1717
HpyAV	CCTTC	5 cut(s)	219, 424, 1256, 1353, 1927
HpyCH4IV	ACGT	2 cut(s)	96, 1722
HpyF10VI	GCNNNNNNNGC	8 cut(s)	90, 235, 329, 341, 1109, 1213, 2218, 2506
HpySE526I	ACGT	2 cut(s)	96, 1722
Hsp92I	GRCGYC	1 cut(s)	96
HspAI	GCGC	2 cut(s)	308, 2172
Ksp22I	TGATCA	1 cut(s)	637
LmnI	GCTCC	2 cut(s)	329, 2209
LweI	GCATC	4 cut(s)	862, 1428, 1879, 2447
MaeI	CTAG	8 cut(s)	666, 1079, 1160, 1496, 1697, 2168, 2246, 2847
MaeII	ACGT	2 cut(s)	96, 1722
MaeIII	GTNAC	2 cut(s)	1810, 1915
MflI	RGATCY	6 cut(s)	527, 668, 1168, 1522, 1612, 2401
MhlI	GDGCHC	2 cut(s)	1516, 1769
MlsI	TGGCCA	2 cut(s)	1434, 1632
MluNI	TGGCCA	2 cut(s)	1434, 1632
MlyI	GAGTC	2 cut(s)	746, 2045
MmeI	TCCRAC	1 cut(s)	1566
Mox20I	TGGCCA	2 cut(s)	1434, 1632
Mph1103I	ATGCAT	1 cut(s)	1872
MroXI	GAANNNNTTC	4 cut(s)	63, 577, 720, 2577
MscI	TGGCCA	2 cut(s)	1434, 1632
MslI	CAYNNNNRTG	3 cut(s)	1092, 1187, 1806
Msp20I	TGGCCA	2 cut(s)	1434, 1632
MspA1I	CMGCKG	2 cut(s)	865, 2041
MspCI	CTTAAG	2 cut(s)	473, 2234
MspI	CCGG	5 cut(s)	311, 856, 1440, 1685, 2492
MspR9I	CCNGG	6 cut(s)	201, 282, 914, 998, 1218, 1441
Mva1269I	GAATGC	1 cut(s)	462
MvaI	CCWGG	5 cut(s)	201, 282, 914, 998, 1218
MwoI	GCNNNNNNNGC	8 cut(s)	90, 235, 329, 341, 1109, 1213, 2218, 2506
NciI	CCSGG	1 cut(s)	1441
NcoI	CCATGG	3 cut(s)	515, 1858, 2361
NlaIV	GGNNCC	3 cut(s)	1267, 2390, 2628
NmeAIII	GCCGAG	2 cut(s)	1943, 2526
NmuCI	GTSAC	1 cut(s)	1915
NsiI	ATGCAT	1 cut(s)	1872
NspI	RCATGY	1 cut(s)	1378
NspV	TTCGAA	1 cut(s)	2667
OliI	CACNNNNGTG	1 cut(s)	1806
PctI	GAATGC	1 cut(s)	462
PdmI	GAANNNNTTC	4 cut(s)	63, 577, 720, 2577
PflMI	CCANNNNNTGG	4 cut(s)	919, 2398, 2516, 2623
PfoI	TCCNGGA	3 cut(s)	199, 280, 996
PleI	GAGTC	2 cut(s)	745, 2045
PpsI	GAGTC	2 cut(s)	745, 2045
PshAI	GACNNNNGTC	1 cut(s)	851
PshBI	ATTAAT	1 cut(s)	2576
Psp124BI	GAGCTC	1 cut(s)	1769
Psp6I	CCWGG	5 cut(s)	199, 280, 912, 996, 1216
PspFI	CCCAGC	2 cut(s)	152, 2872
PspGI	CCWGG	5 cut(s)	199, 280, 912, 996, 1216
PspN4I	GGNNCC	3 cut(s)	1267, 2390, 2628
PspPI	GGNCC	8 cut(s)	512, 853, 1266, 1340, 1469, 2388, 2411, 2626
PsrI	GAACNNNNNNTAC	2 cut(s)	1380, 1412
PstI	CTGCAG	2 cut(s)	1050, 2049
PstNI	CAGNNNCTG	2 cut(s)	2044, 2105
PsuI	RGATCY	6 cut(s)	527, 668, 1168, 1522, 1612, 2401
PvuII	CAGCTG	2 cut(s)	865, 2041
RsaI	GTAC	7 cut(s)	1154, 1193, 1389, 1683, 2131, 2274, 2434
RsaNI	GTAC	7 cut(s)	1153, 1192, 1388, 1682, 2130, 2273, 2433
RseI	CAYNNNNRTG	3 cut(s)	1092, 1187, 1806
SacI	GAGCTC	1 cut(s)	1769
SalI	GTCGAC	1 cut(s)	616
Sau96I	GGNCC	8 cut(s)	512, 853, 1266, 1340, 1469, 2388, 2411, 2626
ScaI	AGTACT	2 cut(s)	2131, 2434
SchI	GAGTC	2 cut(s)	746, 2045
ScrFI	CCNGG	6 cut(s)	201, 282, 914, 998, 1218, 1441
SduI	GDGCHC	2 cut(s)	1516, 1769
SfaNI	GCATC	4 cut(s)	862, 1428, 1879, 2447
SfcI	CTRYAG	5 cut(s)	384, 1046, 1507, 1770, 2045
SfuI	TTCGAA	1 cut(s)	2667
SinI	GGWCC	6 cut(s)	512, 853, 1266, 1340, 2411, 2626
SmiI	ATTTAAAT	1 cut(s)	1092
SmiMI	CAYNNNNRTG	3 cut(s)	1092, 1187, 1806
SmlI	CTYRAG	2 cut(s)	473, 2234
SmoI	CTYRAG	2 cut(s)	473, 2234
SsiI	CCGC	4 cut(s)	908, 1109, 2706, 2915
SspMI	CTAG	8 cut(s)	666, 1079, 1160, 1496, 1697, 2168, 2246, 2847
SstI	GAGCTC	1 cut(s)	1769
StyD4I	CCNGG	6 cut(s)	199, 280, 912, 996, 1216, 1439
StyI	CCWWGG	4 cut(s)	515, 1495, 1858, 2361
SwaI	ATTTAAAT	1 cut(s)	1092
TaiI	ACGT	2 cut(s)	99, 1725
TaqII	GACCGA	1 cut(s)	678
TatI	WGTACW	5 cut(s)	1152, 1191, 2129, 2272, 2432
TauI	GCSGC	3 cut(s)	1112, 2708, 2917
TscAI	CASTG	3 cut(s)	855, 1521, 1767
TseFI	GTSAC	1 cut(s)	1915
Tsp45I	GTSAC	1 cut(s)	1915
TspGWI	ACGGA	3 cut(s)	31, 635, 1730
TspRI	CASTG	3 cut(s)	855, 1521, 1767
Van91I	CCANNNNNTGG	4 cut(s)	919, 2398, 2516, 2623
Vha464I	CTTAAG	2 cut(s)	473, 2234
VpaK11BI	GGWCC	6 cut(s)	512, 853, 1266, 1340, 2411, 2626
VspI	ATTAAT	1 cut(s)	2576
XapI	RAATTY	6 cut(s)	370, 943, 1590, 2596, 2814, 2905
XbaI	TCTAGA	1 cut(s)	665
XceI	RCATGY	1 cut(s)	1378
XmaJI	CCTAGG	1 cut(s)	1495
XmiI	GTMKAC	1 cut(s)	617
XmnI	GAANNNNTTC	4 cut(s)	63, 577, 720, 2577
XspI	CTAG	8 cut(s)	666, 1079, 1160, 1496, 1697, 2168, 2246, 2847
ZraI	GACGTC	1 cut(s)	97
ZrmI	AGTACT	2 cut(s)	2131, 2434
Zsp2I	ATGCAT	1 cut(s)	1872

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.