Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1617 |
| AarI | CACCTGC | 1 cut(s) | 1122 |
| Acc36I | ACCTGC | 1 cut(s) | 1122 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2513, 2963 |
| AccII | CGCG | 2 cut(s) | 2431, 2950 |
| AciI | CCGC | 4 cut(s) | 174, 191, 507, 1330 |
| AclWI | GGATC | 9 cut(s) | 523, 650, 970, 1200, 1471, 1641, 1848, 2478, 2658 |
| AcoI | YGGCCR | 1 cut(s) | 2504 |
| AcuI | CTGAAG | 4 cut(s) | 222, 1713, 2781, 3027 |
| AfaI | GTAC | 8 cut(s) | 699, 1082, 1217, 1689, 1707, 2137, 2365, 2371 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 554, 618, 878, 958, 1381, 2513, 2577, 2963 |
| AflIII | ACRYGT | 2 cut(s) | 2014, 2481 |
| AgeI | ACCGGT | 1 cut(s) | 2509 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 850 |
| AhlI | ACTAGT | 2 cut(s) | 2366, 2786 |
| AjnI | CCWGG | 4 cut(s) | 835, 871, 1083, 1240 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1786, 1818 |
| Alw26I | GTCTC | 2 cut(s) | 80, 858 |
| AlwI | GGATC | 9 cut(s) | 523, 650, 970, 1200, 1471, 1641, 1848, 2478, 2658 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1195, 3002 |
| Ama87I | CYCGRG | 1 cut(s) | 2184 |
| AoxI | GGCC | 6 cut(s) | 295, 838, 970, 1361, 2504, 2891 |
| ApeKI | GCWGC | 8 cut(s) | 137, 329, 905, 1133, 1399, 2180, 2246, 2780 |
| AsiGI | ACCGGT | 1 cut(s) | 2509 |
| Asp700I | GAANNNNTTC | 6 cut(s) | 51, 577, 732, 1337, 1666, 2323 |
| AspA2I | CCTAGG | 1 cut(s) | 1102 |
| AspLEI | GCGC | 1 cut(s) | 2431 |
| AspS9I | GGNCC | 5 cut(s) | 296, 609, 839, 1361, 1490 |
| AsuC2I | CCSGG | 2 cut(s) | 270, 2571 |
| AsuII | TTCGAA | 1 cut(s) | 1660 |
| AsuNHI | GCTAGC | 1 cut(s) | 790 |
| AvaI | CYCGRG | 1 cut(s) | 2184 |
| AvaII | GGWCC | 2 cut(s) | 609, 1490 |
| AvrII | CCTAGG | 1 cut(s) | 1102 |
| BalI | TGGCCA | 1 cut(s) | 2506 |
| BanII | GRGCYC | 2 cut(s) | 198, 2906 |
| BbvI | GCAGC | 8 cut(s) | 149, 341, 892, 1145, 1411, 2167, 2258, 2767 |
| BccI | CCATC | 9 cut(s) | 138, 865, 960, 1277, 1418, 2192, 2587, 2720, 2935 |
| BceAI | ACGGC | 2 cut(s) | 35, 1693 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 53, 87, 1235, 1269, 1742, 1776 |
| BciT130I | CCWGG | 4 cut(s) | 837, 873, 1085, 1242 |
| BciVI | GTATCC | 1 cut(s) | 1136 |
| BclI | TGATCA | 2 cut(s) | 1270, 2767 |
| BcnI | CCSGG | 2 cut(s) | 270, 2571 |
| BcoDI | GTCTC | 2 cut(s) | 80, 858 |
| BcuI | ACTAGT | 2 cut(s) | 2366, 2786 |
| BfaI | CTAG | 8 cut(s) | 470, 695, 791, 1103, 1880, 2367, 2787, 2913 |
| BfmI | CTRYAG | 2 cut(s) | 1776, 2887 |
| BfuAI | ACCTGC | 1 cut(s) | 1122 |
| BfuI | GTATCC | 1 cut(s) | 1136 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 2458, 2989 |
| BisI | GCNGC | 8 cut(s) | 138, 330, 906, 1134, 1400, 2181, 2247, 2781 |
| BlnI | CCTAGG | 1 cut(s) | 1102 |
| BlsI | GCNGC | 8 cut(s) | 139, 331, 907, 1135, 1401, 2182, 2248, 2782 |
| BmcAI | AGTACT | 2 cut(s) | 2365, 2371 |
| Bme1390I | CCNGG | 6 cut(s) | 270, 837, 873, 1085, 1242, 2571 |
| Bme18I | GGWCC | 2 cut(s) | 609, 1490 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 850 |
| BmeT110I | CYCGRG | 1 cut(s) | 2184 |
| BmgT120I | GGNCC | 5 cut(s) | 296, 609, 839, 1361, 1490 |
| BmiI | GGNNCC | 2 cut(s) | 195, 2903 |
| BmrFI | CCNGG | 6 cut(s) | 270, 837, 873, 1085, 1242, 2571 |
| BmsI | GCATC | 8 cut(s) | 793, 1145, 1593, 1856, 1885, 2356, 2478, 2767 |
| BmtI | GCTAGC | 1 cut(s) | 794 |
| BpmI | CTGGAG | 2 cut(s) | 631, 2394 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2251, 2300 |
| Bpu14I | TTCGAA | 1 cut(s) | 1660 |
| BpuEI | CTTGAG | 1 cut(s) | 2627 |
| BpuMI | CCSGG | 2 cut(s) | 270, 2571 |
| BsaAI | YACGTR | 1 cut(s) | 701 |
| BsaJI | CCNNGG | 6 cut(s) | 835, 1102, 1241, 1516, 1747, 1917 |
| BsaWI | WCCGGW | 4 cut(s) | 1460, 1791, 2384, 2509 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1786, 1816, 2034, 2064 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 554, 618, 878, 958, 1381, 2513, 2577, 2963 |
| Bse118I | RCCGGY | 2 cut(s) | 2509, 2893 |
| Bse1I | ACTGG | 7 cut(s) | 497, 648, 813, 973, 1492, 1930, 2377 |
| Bse3DI | GCAATG | 3 cut(s) | 17, 253, 1986 |
| BseBI | CCWGG | 4 cut(s) | 837, 873, 1085, 1242 |
| BseDI | CCNNGG | 6 cut(s) | 835, 1102, 1241, 1516, 1747, 1917 |
| BseGI | GGATG | 4 cut(s) | 149, 931, 2347, 2548 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 554, 618, 878, 958, 1381, 2513, 2577, 2963 |
| BseMI | GCAATG | 3 cut(s) | 17, 253, 1986 |
| BseMII | CTCAG | 7 cut(s) | 91, 749, 840, 1488, 1556, 1983, 2097 |
| BseNI | ACTGG | 7 cut(s) | 497, 648, 813, 973, 1492, 1930, 2377 |
| BseRI | GAGGAG | 3 cut(s) | 788, 1394, 2753 |
| BseXI | GCAGC | 8 cut(s) | 149, 341, 892, 1145, 1411, 2167, 2258, 2767 |
| BsgI | GTGCAG | 2 cut(s) | 1208, 1785 |
| Bsh1236I | CGCG | 2 cut(s) | 2431, 2950 |
| BshFI | GGCC | 6 cut(s) | 297, 840, 972, 1363, 2506, 2893 |
| BshTI | ACCGGT | 1 cut(s) | 2509 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2184 |
| BsiSI | CCGG | 8 cut(s) | 141, 270, 1461, 1792, 2385, 2510, 2571, 2894 |
| BslFI | GGGAC | 2 cut(s) | 82, 2505 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 554, 618, 878, 958, 1381, 2513, 2577, 2963 |
| BsmAI | GTCTC | 2 cut(s) | 80, 858 |
| BsmBI | CGTCTC | 1 cut(s) | 80 |
| BsmFI | GGGAC | 2 cut(s) | 82, 2505 |
| BsmI | GAATGC | 3 cut(s) | 2280, 2491, 2992 |
| BsnI | GGCC | 6 cut(s) | 297, 840, 972, 1363, 2506, 2893 |
| BsoBI | CYCGRG | 1 cut(s) | 2184 |
| Bsp119I | TTCGAA | 1 cut(s) | 1660 |
| Bsp1286I | GDGCHC | 2 cut(s) | 198, 2906 |
| Bsp68I | TCGCGA | 1 cut(s) | 2950 |
| BspACI | CCGC | 4 cut(s) | 174, 191, 507, 1330 |
| BspANI | GGCC | 6 cut(s) | 297, 840, 972, 1363, 2506, 2893 |
| BspCNI | CTCAG | 7 cut(s) | 90, 750, 841, 1489, 1557, 1982, 2098 |
| BspFNI | CGCG | 2 cut(s) | 2431, 2950 |
| BspHI | TCATGA | 1 cut(s) | 591 |
| BspLI | GGNNCC | 2 cut(s) | 195, 2903 |
| BspMAI | CTGCAG | 1 cut(s) | 2891 |
| BspMI | ACCTGC | 1 cut(s) | 1122 |
| BspOI | GCTAGC | 1 cut(s) | 794 |
| BspPI | GGATC | 9 cut(s) | 523, 650, 970, 1200, 1471, 1641, 1848, 2478, 2658 |
| BspQI | GCTCTTC | 1 cut(s) | 2341 |
| BspT104I | TTCGAA | 1 cut(s) | 1660 |
| BsrDI | GCAATG | 3 cut(s) | 17, 253, 1986 |
| BsrFI | RCCGGY | 2 cut(s) | 2509, 2893 |
| BsrI | ACTGG | 7 cut(s) | 497, 648, 813, 973, 1492, 1930, 2377 |
| BssAI | RCCGGY | 2 cut(s) | 2509, 2893 |
| BssECI | CCNNGG | 6 cut(s) | 835, 1102, 1241, 1516, 1747, 1917 |
| BssT1I | CCWWGG | 2 cut(s) | 1102, 1747 |
| Bst2UI | CCWGG | 4 cut(s) | 837, 873, 1085, 1242 |
| Bst4CI | ACNGT | 5 cut(s) | 160, 464, 1822, 2167, 2946 |
| Bst6I | CTCTTC | 2 cut(s) | 891, 2341 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 19 |
| BstBAI | YACGTR | 1 cut(s) | 701 |
| BstBI | TTCGAA | 1 cut(s) | 1660 |
| BstC8I | GCNNGC | 7 cut(s) | 189, 505, 792, 796, 2073, 2332, 2891 |
| BstF5I | GGATG | 4 cut(s) | 149, 931, 2347, 2548 |
| BstFNI | CGCG | 2 cut(s) | 2431, 2950 |
| BstHHI | GCGC | 1 cut(s) | 2431 |
| BstMAI | GTCTC | 2 cut(s) | 80, 858 |
| BstNI | CCWGG | 4 cut(s) | 837, 873, 1085, 1242 |
| BstNSI | RCATGY | 2 cut(s) | 694, 2485 |
| BstSCI | CCNGG | 6 cut(s) | 268, 835, 871, 1083, 1240, 2569 |
| BstSFI | CTRYAG | 2 cut(s) | 1776, 2887 |
| BstUI | CGCG | 2 cut(s) | 2431, 2950 |
| BstV1I | GCAGC | 8 cut(s) | 149, 341, 892, 1145, 1411, 2167, 2258, 2767 |
| BstX2I | RGATCY | 5 cut(s) | 655, 1192, 1633, 1840, 2470 |
| BstYI | RGATCY | 5 cut(s) | 655, 1192, 1633, 1840, 2470 |
| BsuI | GTATCC | 1 cut(s) | 1136 |
| BsuRI | GGCC | 6 cut(s) | 297, 840, 972, 1363, 2506, 2893 |
| BtgZI | GCGATG | 1 cut(s) | 96 |
| BtsCI | GGATG | 4 cut(s) | 149, 931, 2347, 2548 |
| BtsI | GCAGTG | 1 cut(s) | 1773 |
| BtsIMutI | CAGTG | 2 cut(s) | 655, 1773 |
| BtuMI | TCGCGA | 1 cut(s) | 2950 |
| BveI | ACCTGC | 1 cut(s) | 1122 |
| Cac8I | GCNNGC | 7 cut(s) | 189, 505, 792, 796, 2073, 2332, 2891 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1195, 3002 |
| CciI | TCATGA | 1 cut(s) | 591 |
| CfoI | GCGC | 1 cut(s) | 2431 |
| Cfr10I | RCCGGY | 2 cut(s) | 2509, 2893 |
| Cfr13I | GGNCC | 5 cut(s) | 296, 609, 839, 1361, 1490 |
| CseI | GACGC | 1 cut(s) | 1217 |
| CsiI | ACCWGGT | 1 cut(s) | 871 |
| Csp6I | GTAC | 8 cut(s) | 698, 1081, 1216, 1688, 1706, 2136, 2364, 2370 |
| CspAI | ACCGGT | 1 cut(s) | 2509 |
| CviQI | GTAC | 8 cut(s) | 698, 1081, 1216, 1688, 1706, 2136, 2364, 2370 |
| DriI | GACNNNNNGTC | 1 cut(s) | 850 |
| EaeI | YGGCCR | 1 cut(s) | 2504 |
| Eam1104I | CTCTTC | 2 cut(s) | 891, 2341 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 850 |
| EarI | CTCTTC | 2 cut(s) | 891, 2341 |
| EciI | GGCGGA | 2 cut(s) | 522, 1345 |
| Eco130I | CCWWGG | 2 cut(s) | 1102, 1747 |
| Eco24I | GRGCYC | 2 cut(s) | 198, 2906 |
| Eco32I | GATATC | 1 cut(s) | 886 |
| Eco47I | GGWCC | 2 cut(s) | 609, 1490 |
| Eco57I | CTGAAG | 4 cut(s) | 222, 1713, 2781, 3027 |
| Eco88I | CYCGRG | 1 cut(s) | 2184 |
| EcoO109I | RGGNCCY | 2 cut(s) | 296, 609 |
| EcoRI | GAATTC | 1 cut(s) | 2323 |
| EcoRII | CCWGG | 4 cut(s) | 835, 871, 1083, 1240 |
| EcoRV | GATATC | 1 cut(s) | 886 |
| EcoT14I | CCWWGG | 2 cut(s) | 1102, 1747 |
| EcoT22I | ATGCAT | 3 cut(s) | 1878, 2349, 2493 |
| EcoT38I | GRGCYC | 2 cut(s) | 198, 2906 |
| ErhI | CCWWGG | 2 cut(s) | 1102, 1747 |
| Esp3I | CGTCTC | 1 cut(s) | 80 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 39, 71, 1686, 1718, 2046, 2078, 2234, 2266 |
| FaqI | GGGAC | 2 cut(s) | 82, 2505 |
| FauNDI | CATATG | 2 cut(s) | 946, 2717 |
| FbaI | TGATCA | 2 cut(s) | 1270, 2767 |
| Fnu4HI | GCNGC | 8 cut(s) | 138, 330, 906, 1134, 1400, 2181, 2247, 2781 |
| FokI | GGATG | 4 cut(s) | 156, 938, 2334, 2555 |
| FriOI | GRGCYC | 2 cut(s) | 198, 2906 |
| Fsp4HI | GCNGC | 8 cut(s) | 138, 330, 906, 1134, 1400, 2181, 2247, 2781 |
| FspBI | CTAG | 8 cut(s) | 470, 695, 791, 1103, 1880, 2367, 2787, 2913 |
| GlaI | GCGC | 1 cut(s) | 2430 |
| GluI | GCNGC | 8 cut(s) | 138, 330, 906, 1134, 1400, 2181, 2247, 2781 |
| GsuI | CTGGAG | 2 cut(s) | 631, 2394 |
| HaeIII | GGCC | 6 cut(s) | 297, 840, 972, 1363, 2506, 2893 |
| HapII | CCGG | 8 cut(s) | 141, 270, 1461, 1792, 2385, 2510, 2571, 2894 |
| HgaI | GACGC | 1 cut(s) | 1217 |
| HhaI | GCGC | 1 cut(s) | 2431 |
| Hin6I | GCGC | 1 cut(s) | 2429 |
| HinP1I | GCGC | 1 cut(s) | 2429 |
| HincII | GTYRAC | 3 cut(s) | 460, 1885, 2191 |
| HindII | GTYRAC | 3 cut(s) | 460, 1885, 2191 |
| HindIII | AAGCTT | 3 cut(s) | 114, 2603, 2976 |
| HpaII | CCGG | 8 cut(s) | 141, 270, 1461, 1792, 2385, 2510, 2571, 2894 |
| Hpy166II | GTNNAC | 9 cut(s) | 460, 1216, 1831, 1885, 2036, 2095, 2191, 2599, 2659 |
| Hpy8I | GTNNAC | 9 cut(s) | 460, 1216, 1831, 1885, 2036, 2095, 2191, 2599, 2659 |
| Hpy99I | CGWCG | 1 cut(s) | 2950 |
| HpyCH4III | ACNGT | 5 cut(s) | 160, 464, 1822, 2167, 2946 |
| HpyCH4IV | ACGT | 4 cut(s) | 360, 700, 1686, 2016 |
| HpySE526I | ACGT | 4 cut(s) | 360, 700, 1686, 2016 |
| HspAI | GCGC | 1 cut(s) | 2429 |
| Ksp22I | TGATCA | 2 cut(s) | 1270, 2767 |
| LguI | GCTCTTC | 1 cut(s) | 2341 |
| LmnI | GCTCC | 6 cut(s) | 10, 193, 272, 2833, 2864, 2901 |
| Lsp1109I | GCAGC | 8 cut(s) | 149, 341, 892, 1145, 1411, 2167, 2258, 2767 |
| LweI | GCATC | 8 cut(s) | 793, 1145, 1593, 1856, 1885, 2356, 2478, 2767 |
| MabI | ACCWGGT | 1 cut(s) | 871 |
| MaeI | CTAG | 8 cut(s) | 470, 695, 791, 1103, 1880, 2367, 2787, 2913 |
| MaeII | ACGT | 4 cut(s) | 360, 700, 1686, 2016 |
| MaeIII | GTNAC | 6 cut(s) | 302, 488, 1300, 1427, 1501, 1921 |
| MfeI | CAATTG | 1 cut(s) | 1296 |
| MflI | RGATCY | 5 cut(s) | 655, 1192, 1633, 1840, 2470 |
| MhlI | GDGCHC | 2 cut(s) | 198, 2906 |
| MlsI | TGGCCA | 1 cut(s) | 2506 |
| MluNI | TGGCCA | 1 cut(s) | 2506 |
| MlyI | GAGTC | 8 cut(s) | 64, 299, 839, 860, 2051, 2196, 2701, 2752 |
| MmeI | TCCRAC | 3 cut(s) | 142, 425, 511 |
| Mox20I | TGGCCA | 1 cut(s) | 2506 |
| Mph1103I | ATGCAT | 3 cut(s) | 1878, 2349, 2493 |
| MroXI | GAANNNNTTC | 6 cut(s) | 51, 577, 732, 1337, 1666, 2323 |
| MscI | TGGCCA | 1 cut(s) | 2506 |
| MseI | TTAA | 8 cut(s) | 27, 255, 1683, 1937, 1956, 2211, 2636, 2835 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1116, 2486, 2500 |
| Msp20I | TGGCCA | 1 cut(s) | 2506 |
| MspA1I | CMGCKG | 1 cut(s) | 191 |
| MspI | CCGG | 8 cut(s) | 141, 270, 1461, 1792, 2385, 2510, 2571, 2894 |
| MspR9I | CCNGG | 6 cut(s) | 270, 837, 873, 1085, 1242, 2571 |
| MunI | CAATTG | 1 cut(s) | 1296 |
| Mva1269I | GAATGC | 3 cut(s) | 2280, 2491, 2992 |
| MvaI | CCWGG | 4 cut(s) | 837, 873, 1085, 1242 |
| MvnI | CGCG | 2 cut(s) | 2431, 2950 |
| NciI | CCSGG | 2 cut(s) | 270, 2571 |
| NdeI | CATATG | 2 cut(s) | 946, 2717 |
| NheI | GCTAGC | 1 cut(s) | 790 |
| NlaIV | GGNNCC | 2 cut(s) | 195, 2903 |
| NmeAIII | GCCGAG | 2 cut(s) | 1247, 1541 |
| NmuCI | GTSAC | 4 cut(s) | 302, 488, 1300, 1921 |
| NruI | TCGCGA | 1 cut(s) | 2950 |
| NsiI | ATGCAT | 3 cut(s) | 1878, 2349, 2493 |
| NspI | RCATGY | 2 cut(s) | 694, 2485 |
| NspV | TTCGAA | 1 cut(s) | 1660 |
| PaeR7I | CTCGAG | 1 cut(s) | 2184 |
| PagI | TCATGA | 1 cut(s) | 591 |
| PaqCI | CACCTGC | 1 cut(s) | 1122 |
| PciI | ACATGT | 1 cut(s) | 2481 |
| PciSI | GCTCTTC | 1 cut(s) | 2341 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1683 |
| PctI | GAATGC | 3 cut(s) | 2280, 2491, 2992 |
| PdmI | GAANNNNTTC | 6 cut(s) | 51, 577, 732, 1337, 1666, 2323 |
| PfeI | GAWTC | 5 cut(s) | 749, 910, 1759, 2759, 2792 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2513, 2963 |
| PinAI | ACCGGT | 1 cut(s) | 2509 |
| PkrI | GCNGC | 8 cut(s) | 139, 331, 907, 1135, 1401, 2182, 2248, 2782 |
| PleI | GAGTC | 8 cut(s) | 64, 299, 839, 859, 2051, 2195, 2700, 2751 |
| PpsI | GAGTC | 8 cut(s) | 64, 299, 839, 859, 2051, 2195, 2700, 2751 |
| Ppu21I | YACGTR | 1 cut(s) | 701 |
| PpuMI | RGGWCCY | 1 cut(s) | 609 |
| PscI | ACATGT | 1 cut(s) | 2481 |
| PsiI | TTATAA | 1 cut(s) | 1617 |
| Psp5II | RGGWCCY | 1 cut(s) | 609 |
| Psp6I | CCWGG | 4 cut(s) | 835, 871, 1083, 1240 |
| PspGI | CCWGG | 4 cut(s) | 835, 871, 1083, 1240 |
| PspN4I | GGNNCC | 2 cut(s) | 195, 2903 |
| PspPI | GGNCC | 5 cut(s) | 296, 609, 839, 1361, 1490 |
| PspPPI | RGGWCCY | 1 cut(s) | 609 |
| PspXI | VCTCGAGB | 1 cut(s) | 2184 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 690, 722 |
| PstI | CTGCAG | 1 cut(s) | 2891 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1195, 3002 |
| PsuI | RGATCY | 5 cut(s) | 655, 1192, 1633, 1840, 2470 |
| RruI | TCGCGA | 1 cut(s) | 2950 |
| RsaI | GTAC | 8 cut(s) | 699, 1082, 1217, 1689, 1707, 2137, 2365, 2371 |
| RsaNI | GTAC | 8 cut(s) | 698, 1081, 1216, 1688, 1706, 2136, 2364, 2370 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1116, 2486, 2500 |
| SapI | GCTCTTC | 1 cut(s) | 2341 |
| SaqAI | TTAA | 8 cut(s) | 27, 255, 1683, 1937, 1956, 2211, 2636, 2835 |
| SatI | GCNGC | 8 cut(s) | 138, 330, 906, 1134, 1400, 2181, 2247, 2781 |
| Sau96I | GGNCC | 5 cut(s) | 296, 609, 839, 1361, 1490 |
| ScaI | AGTACT | 2 cut(s) | 2365, 2371 |
| SchI | GAGTC | 8 cut(s) | 64, 299, 839, 860, 2051, 2196, 2701, 2752 |
| ScrFI | CCNGG | 6 cut(s) | 270, 837, 873, 1085, 1242, 2571 |
| SduI | GDGCHC | 2 cut(s) | 198, 2906 |
| SexAI | ACCWGGT | 1 cut(s) | 871 |
| SfaNI | GCATC | 8 cut(s) | 793, 1145, 1593, 1856, 1885, 2356, 2478, 2767 |
| SfcI | CTRYAG | 2 cut(s) | 1776, 2887 |
| Sfr274I | CTCGAG | 1 cut(s) | 2184 |
| SfuI | TTCGAA | 1 cut(s) | 1660 |
| SinI | GGWCC | 2 cut(s) | 609, 1490 |
| SlaI | CTCGAG | 1 cut(s) | 2184 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1116, 2486, 2500 |
| SmlI | CTYRAG | 2 cut(s) | 2184, 2606 |
| SmoI | CTYRAG | 2 cut(s) | 2184, 2606 |
| SpeI | ACTAGT | 2 cut(s) | 2366, 2786 |
| SsiI | CCGC | 4 cut(s) | 174, 191, 507, 1330 |
| SspI | AATATT | 1 cut(s) | 1018 |
| SspMI | CTAG | 8 cut(s) | 470, 695, 791, 1103, 1880, 2367, 2787, 2913 |
| StyD4I | CCNGG | 6 cut(s) | 268, 835, 871, 1083, 1240, 2569 |
| StyI | CCWWGG | 2 cut(s) | 1102, 1747 |
| TaaI | ACNGT | 5 cut(s) | 160, 464, 1822, 2167, 2946 |
| TaiI | ACGT | 4 cut(s) | 363, 703, 1689, 2019 |
| TaqI | TCGA | 2 cut(s) | 1660, 2185 |
| TaqII | GACCGA | 2 cut(s) | 1401, 1507 |
| TatI | WGTACW | 3 cut(s) | 1215, 2363, 2369 |
| TfiI | GAWTC | 5 cut(s) | 749, 910, 1759, 2759, 2792 |
| Tru1I | TTAA | 8 cut(s) | 27, 255, 1683, 1937, 1956, 2211, 2636, 2835 |
| Tru9I | TTAA | 8 cut(s) | 27, 255, 1683, 1937, 1956, 2211, 2636, 2835 |
| TscAI | CASTG | 2 cut(s) | 655, 1773 |
| TseFI | GTSAC | 4 cut(s) | 302, 488, 1300, 1921 |
| TseI | GCWGC | 8 cut(s) | 137, 329, 905, 1133, 1399, 2180, 2246, 2780 |
| Tsp45I | GTSAC | 4 cut(s) | 302, 488, 1300, 1921 |
| TspGWI | ACGGA | 5 cut(s) | 23, 1585, 1848, 2010, 2420 |
| TspRI | CASTG | 2 cut(s) | 655, 1773 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2513, 2963 |
| VpaK11BI | GGWCC | 2 cut(s) | 609, 1490 |
| XceI | RCATGY | 2 cut(s) | 694, 2485 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 2060, 2811 |
| XhoI | CTCGAG | 1 cut(s) | 2184 |
| XmaJI | CCTAGG | 1 cut(s) | 1102 |
| XmnI | GAANNNNTTC | 6 cut(s) | 51, 577, 732, 1337, 1666, 2323 |
| XspI | CTAG | 8 cut(s) | 470, 695, 791, 1103, 1880, 2367, 2787, 2913 |
| ZrmI | AGTACT | 2 cut(s) | 2365, 2371 |
| Zsp2I | ATGCAT | 3 cut(s) | 1878, 2349, 2493 |