Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001083

Physical Location & Seq

Genomic Coordinates Forward (+)

28842138 .. 28847340

Gene Structure 5203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1209400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3081 bp

ATGGCGACGGAGCAAGTCTTGCCGTTCTCCATAGCTTCTGTGGTTGAATGCGTTCTTCAGAAGCATGGGAGTCGTTTAAACGATATCGACTTGGCTTCTAGGAAGGCCGAGGAAGCATCGTTGAGAAGGTATGAAGCAGCAAGGTGGCTAAGAAAGACCGTTGGCGTCGTTGGGGGTAAAGATTTGCCGGCTGAGCCTTCGGAAGAGGATTTCCGCCTCGGATTGCGCAGTGGAATAATCCTCTGCAATGTTCTCAACAAAGTTCAACCCGGAGCTGTGCCGAAGGTAGTTGAAGGCCCTTGTGACTCCGTTATTATTCCCGATGGGGCGGCTTTGTCAGCGTATCAGTACTTCGAAAATGTCAGGAACTTCCTTGTGTCTGTTGAGGAAATGGGGCTTCCAACCTTTGAAGCCTCGGATTTGGAACAGGGAGGGAAATCCGCAAGGATTGTGAACTGCGTTCTAGCATTGAAATCATACTCTGAGTGGAAACAAGGAGGTGCAATTGGATCGTGGAAATATTGTGGAGCTTTGAAACCAACTACCTGCGGGAAATACTTCGTGCGAAAAAATTCAGAACCCTTTTCAAATTCCTTTCCAATGACCTTATCATGGGGCGAAAAGTCCCACGATAGTTTGTCCAGTGAAGATCTCAATGAAGCAAGTTCCTCTCATTCTTTACATGTGCTAGTCCGTGAACTGCTTTCAGACAAGAGGCAGGAAGACATACCAATCATTGTGGAAAATATGCTGAGTAAAGTCATGGAGGAGTTTGAGCATCGGTTAGCAAGCCAAGGCGAACTGATGAAAACAGCTTCCAGAGATATGACGTTGTCCGGCCCTGATAGACCTCTTTCGGACTGTGCCTCTGAATGTGCTTCTGGTGATATGAAGATTGAAGAGAAAGCTTCCTCTGAAACCAAAGAGGAGGAAATATACAACCAAAGCGAGGTATCGAAAAGTCAGTCTTTAAAGCAGATAACGTTGGTTGATCGACAACAGAAAGACATCCAGGAATTGAAACATACTCTATACGATACAAAAGCAGGAATGAAGCTTCTACAAATGAGGTACCAGCAGGAGTTCAACAACCTAGGCAAGCACCTCCATGGTTTGGCTTCTGCAGCGACAGGATATCAGAAGGTTCTTGAAGAAAATCGCAAGTTATACAATCAAGTGCAGGATTTGAAAGGAAATATAAGGGTATACTGTCGAGTGCGGCCCTTCTTGCCTGGGCAACCAAATCGGGCCAGTACTTATGATCACATAGATGACAGAAGTCTTAAAATTATCACTCCCTCCAAATATGGCAAAGAGGGAAGGAAATCTTTCACTTTCAACAAAGTTTTTGGTCCCTTTTCAACCCAAGAGGAGGTGTTTTCAGATACGCAGCCTCTGATTCGGTCTGTTCTCGATGGTTACAATGTCTGTATATTTGCTTATGGCCAAACGGGATCAGGGAAAACTTTTACAATGACTGGACCGAAGGAGATTACAGAGGAAAGTCGAGGTGTAAATTACAGAGCACTCAGTGATTTATTTTATCTATCAGAACAGAGAAAGGACACCATTTGTTACGAAATTTCTGTTCAGATGCTTGAAATTTACAATGAGCAAGTCAAGGATCTCCTTGCGGTGGAGGAAATTCGCAACAGCTCTCAAAACGGAATTAATGTACCTGAGGCGAACCTTGTTCCCGTATCATCGACTTCTGATGTTATATATTTGATGAACCTTGGGCATAAGAATCGTTCAGTGAGTGCCACAGCCATGAATGACAGGAGTAGTAGGTCGCACAGTTGCTTGACAGTACATATTCACGGAAGAGACCTGACGTCAGGAAGTATTCTTCGGGGTTGTATGCATCTAGTTGACTTGGCAGGAAGTGAGAGAGTTGACAAATCTGAGGTGACGGGAGACAGGTTAAAGGAGGCACAACATATTAACAAATCTCTCTCAGCTTTAGGAGATGTCATTGCCTCTCTTGCTCAAAAGAACGCACACGTTCCTTACAGAAACAGTAAACTTACACAACTACTCCAAGACTCACTCGGAGGGCAAGCAAAGACGCTCATGTTTGTTCATATCAGCCCTGAGCCTGACGCTCTCGGAGAAACACTTAGTACACTCAAATTTGCAGAAAGAGTTTCCACAGTTGAACTTGGTGCTGCTCGAGTTAACAAAGACGGTGCAGATGTAAAAGAACTCAAAGAACAGATTGCTACACTTAAGGCAGCCTTAGCAAGGAAGGAAAGCGAGGGAGCGCAAGCGCAACTTAAATATTCTCAATCCATGAGCCCAGAAAGATCTAGAATCAAGTCTGCTGGGTCTTCCCCTTTGCATTTTAATCGGAAAAGCACTGGAGATTTGGCCGGGGCTCGCAGGCAAACAGCAGATGATTTTGGAAACATAGAGGTTCGCAAAAACACTGCATTGAAGCCAAAAAGGCGAAGTTTAGATCCTCAAGATATGATGATGCATGCACGTTCGCCTCCCTGGCCACCTGCTTGTAATCAAGGACTAAATTCAAAGGAGGAATCCGATAAGGATTCAGTACTTTCTGGAGATTGGGTTGATAAGGTCATGGTGAACAAACATGAAGGTGCAAATAGAGAAGAGAATCTATTAGGAAGTTGGGATATGGACAATAGACAGTTGCCGGAGATGTTTGGACCGAGTAGCCTTCCTGACCCTCCGAAGTTGTACATGGAACAACACTTTGGAAAATTAACGGCGAGCAAAGAGGACAGCCAAGACTATGATGTACAAAGGAGTCGGAATGAGATGGCCAATACCGATGATTCTGATGATCAGCTTGATGCTGCAACAAGTGATTGTTCAGAGCCAGACATGCTTTGGCAGCAAAACCTTCCGAAACCTACCAGCATTCCAAACGGTCTGAGGGCAAAAGCAAAGAAAACTAATTCGAGGCCAATAAGGAGCCCGGAAACTAGGAGTATGATTCCATCGTTTATTCCTTCGCCATCACGGAAACCACCCAATGGAGTTAGTCAGCCTCTGCACAGGACCTTAAGTGGAAGGTCCGGTGAAGGGAAACGAAGGCCTGGGAGTGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0008569 GO:0009987 GO:0015629 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031534 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043621 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0046907 GO:0051012 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051647 GO:0051649 GO:0051656 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

1027

Amino Acids

113.92

Weight (kDa)

7.08

Isoelectric Point (pI)

52.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	43 - 161	9e-13	Calponin homology (CH) domain
Microtub_bd	PF16796	384 - 544	1.8e-44	Microtubule binding
Kinesin	PF00225	405 - 718	4.5e-107	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001083)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT1G09170 AT5G27000
fragaria_vesca	FvH4_2g14960 FvH4_6g07070 FvH4_6g07070 FvH4_6g07070 FvH4_6g07070 FvH4_6g07070
malus_domestica	MD04G1196400.v1.1 MD12G1209400.v1.1
prunus_persica	Prupe.6G316600_v2.0.a1
pyrus_communis	pycom12g19670
rosa_chinensis	RchiOBHm_Chr1g0318271 RchiOBHm_Chr2g0102121 RchiOBHm_Chr2g0167941 RchiOBHm_Chr3g0456621 RchiOBHm_Chr3g0456641 RchiOBHm_Chr5g0067221
rosa_laevigata	RLG00000003185 RLG00000003285 RLG00000003859 RLG00000003890 RLG00000025287 RLG00000033013
rosa_multiflora	Rmu_sc0000133.1_g000037 Rmu_sc0000894.1_g000040 Rmu_sc0001563.1_g000026 Rmu_sc0007742.1_g000030 Rmu_sc0012002.1_g000001
rosa_roxburghii	Rroxscaffold_2G00120300 Rroxscaffold_2G00132430 Rroxscaffold_5G00367570 Rroxscaffold_6G00422580 Rroxscaffold_7G00200730
rosa_rugosa	Rorug03G0014400 Rorug05G0465400 Rorug05G0465500
rosa_samantha	Rh1AG324900 Rh1CG231300 Rh2AG394600 Rh3AG075200 Rh3BG077100 Rh3CG076700 Rh3CG076800 Rh3DG078700 Rh5BG530400 Rh5CG554800 Rh6AG058800
rosa_wichuraiana	Rw3G006440 Rw4G008320 Rw5G014820 Rw7G027120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2512
AatII	GACGTC	1 cut(s)	1838
Acc16I	TGCGCA	1 cut(s)	227
Acc36I	ACCTGC	2 cut(s)	554, 2512
Acc65I	GGTACC	1 cut(s)	1071
AccB1I	GGYRCC	1 cut(s)	1071
AccB7I	CCANNNNNTGG	2 cut(s)	1114, 3002
AccI	GTMKAC	1 cut(s)	1206
AciI	CCGC	6 cut(s)	214, 329, 441, 549, 1219, 1634
AclI	AACGTT	1 cut(s)	983
AclWI	GGATC	4 cut(s)	517, 1462, 1632, 2453
AcoI	YGGCCR	4 cut(s)	1444, 2370, 2498, 2787
AcsI	RAATTY	7 cut(s)	571, 589, 1581, 1602, 1644, 2132, 2524
AcuI	CTGAAG	1 cut(s)	41
AcyI	GRCGYC	2 cut(s)	165, 1835
AdeI	CACNNNGTG	1 cut(s)	1532
AfaI	GTAC	9 cut(s)	350, 1073, 1255, 1677, 1812, 2125, 2556, 2705, 2766
AfiI	CCNNNNNNNGG	8 cut(s)	612, 949, 1114, 1372, 1636, 2244, 3002, 3051
AflII	CTTAAG	2 cut(s)	2228, 3031
AflIII	ACRYGT	2 cut(s)	682, 2002
AhdI	GACNNNNNGTC	1 cut(s)	1834
AjnI	CCWGG	4 cut(s)	1011, 1231, 2495, 3064
AjuI	GAANNNNNNNTTGG	2 cut(s)	2703, 2735
AluBI	AGCT	9 cut(s)	35, 275, 530, 815, 908, 1057, 1656, 1961, 2815
AluI	AGCT	9 cut(s)	35, 275, 530, 815, 908, 1057, 1656, 1961, 2815
Alw21I	GWGCWC	1 cut(s)	1528
Alw26I	GTCTC	2 cut(s)	1821, 1911
AlwI	GGATC	4 cut(s)	517, 1462, 1632, 2453
AlwNI	CAGNNNCTG	2 cut(s)	1396, 3019
Ama87I	CYCGRG	1 cut(s)	2172
ApeKI	GCWGC	7 cut(s)	137, 1124, 1390, 2168, 2234, 2822, 2860
ApoI	RAATTY	7 cut(s)	571, 589, 1581, 1602, 1644, 2132, 2524
ArsI	GACNNNNNNTTYG	2 cut(s)	950, 982
AseI	ATTAAT	1 cut(s)	1671
Asp700I	GAANNNNTTC	4 cut(s)	51, 557, 571, 1328
Asp718I	GGTACC	1 cut(s)	1071
AspA2I	CCTAGG	1 cut(s)	1093
AspLEI	GCGC	3 cut(s)	228, 2266, 2272
AspS9I	GGNCC	9 cut(s)	296, 839, 1221, 1248, 1352, 1481, 2672, 3027, 3042
AsuC2I	CCSGG	3 cut(s)	270, 2374, 2945
AsuHPI	GGTGA	4 cut(s)	896, 1921, 2599, 3059
AsuII	TTCGAA	1 cut(s)	354
AvaI	CYCGRG	1 cut(s)	2172
AvaII	GGWCC	5 cut(s)	1352, 1481, 2672, 3027, 3042
AvrII	CCTAGG	1 cut(s)	1093
AxyI	CCTNAGG	1 cut(s)	1680
BalI	TGGCCA	3 cut(s)	1446, 2500, 2789
BanI	GGYRCC	1 cut(s)	1071
BanII	GRGCYC	3 cut(s)	2300, 2380, 2945
BarI	GAAGNNNNNNTAC	2 cut(s)	335, 367
BbsI	GAAGAC	2 cut(s)	729, 2322
Bbv12I	GWGCWC	1 cut(s)	1528
BbvI	GCAGC	7 cut(s)	149, 1136, 1402, 2155, 2246, 2809, 2872
BccI	CCATC	5 cut(s)	317, 1409, 2779, 2974, 2992
BceAI	ACGGC	2 cut(s)	7, 2748
BciT130I	CCWGG	4 cut(s)	1013, 1233, 2497, 3066
BclI	TGATCA	2 cut(s)	1261, 2809
BcnI	CCSGG	3 cut(s)	270, 2374, 2945
BcoDI	GTCTC	2 cut(s)	1821, 1911
BfaI	CTAG	7 cut(s)	99, 464, 689, 1094, 1868, 2310, 2952
BfmI	CTRYAG	1 cut(s)	1122
BfrI	CTTAAG	2 cut(s)	2228, 3031
BfuAI	ACCTGC	2 cut(s)	554, 2512
BglI	GCCNNNNNGGC	2 cut(s)	2446, 2497
BglII	AGATCT	2 cut(s)	649, 2306
BisI	GCNGC	9 cut(s)	138, 330, 1125, 1220, 1391, 2169, 2235, 2823, 2861
BlnI	CCTAGG	1 cut(s)	1093
BlpI	GCTNAGC	1 cut(s)	192
BlsI	GCNGC	9 cut(s)	139, 331, 1126, 1221, 1392, 2170, 2236, 2824, 2862
BmcAI	AGTACT	3 cut(s)	350, 1255, 2556
Bme1390I	CCNGG	7 cut(s)	270, 1013, 1233, 2374, 2497, 2945, 3066
Bme18I	GGWCC	5 cut(s)	1352, 1481, 2672, 3027, 3042
BmeRI	GACNNNNNGTC	1 cut(s)	1834
BmeT110I	CYCGRG	1 cut(s)	2172
BmgT120I	GGNCC	9 cut(s)	296, 839, 1221, 1248, 1352, 1481, 2672, 3027, 3042
BmiI	GGNNCC	3 cut(s)	1073, 1354, 2942
BmrFI	CCNGG	7 cut(s)	270, 1013, 1233, 2374, 2497, 2945, 3066
BmsI	GCATC	6 cut(s)	125, 787, 1584, 1873, 2466, 2809
BoxI	GACNNNNGTC	1 cut(s)	1278
BpiI	GAAGAC	2 cut(s)	729, 2322
BpmI	CTGGAG	2 cut(s)	2382, 2583
Bpu10I	CCTNAGC	2 cut(s)	2094, 2239
Bpu1102I	GCTNAGC	1 cut(s)	192
Bpu14I	TTCGAA	1 cut(s)	354
BpuEI	CTTGAG	1 cut(s)	2448
BpuMI	CCSGG	3 cut(s)	270, 2374, 2945
BsaHI	GRCGYC	2 cut(s)	165, 1835
BsaI	GGTCTC	1 cut(s)	1821
BsaWI	WCCGGW	1 cut(s)	3044
BsaXI	ACNNNNNCTCC	4 cut(s)	2022, 2052, 2556, 2586
Bsc4I	CCNNNNNNNGG	8 cut(s)	612, 949, 1114, 1372, 1636, 2244, 3002, 3051
Bse118I	RCCGGY	1 cut(s)	187
Bse1I	ACTGG	4 cut(s)	642, 1251, 1483, 2365
Bse21I	CCTNAGG	1 cut(s)	1680
Bse3DI	GCAATG	2 cut(s)	253, 1974
BseBI	CCWGG	4 cut(s)	1013, 1233, 2497, 3066
BseGI	GGATG	1 cut(s)	1008
BseLI	CCNNNNNNNGG	8 cut(s)	612, 949, 1114, 1372, 1636, 2244, 3002, 3051
BseMI	GCAATG	2 cut(s)	253, 1974
BseMII	CTCAG	9 cut(s)	183, 474, 743, 1543, 1671, 1896, 1971, 2085, 2891
BseNI	ACTGG	4 cut(s)	642, 1251, 1483, 2365
BseRI	GAGGAG	3 cut(s)	782, 941, 1385
BseXI	GCAGC	7 cut(s)	149, 1136, 1402, 2155, 2246, 2809, 2872
BseYI	CCCAGC	1 cut(s)	2324
BsgI	GTGCAG	3 cut(s)	1199, 2211, 3005
BshNI	GGYRCC	1 cut(s)	1071
BsiHKAI	GWGCWC	1 cut(s)	1528
BsiHKCI	CYCGRG	1 cut(s)	2172
BsiSI	CCGG	7 cut(s)	188, 270, 837, 2373, 2660, 2945, 3045
BslFI	GGGAC	2 cut(s)	610, 1338
BslI	CCNNNNNNNGG	8 cut(s)	612, 949, 1114, 1372, 1636, 2244, 3002, 3051
BsmAI	GTCTC	2 cut(s)	1821, 1911
BsmFI	GGGAC	2 cut(s)	610, 1338
BsmI	GAATGC	2 cut(s)	53, 2886
Bso31I	GGTCTC	1 cut(s)	1821
BsoBI	CYCGRG	1 cut(s)	2172
Bsp119I	TTCGAA	1 cut(s)	354
Bsp1286I	GDGCHC	4 cut(s)	1528, 2300, 2380, 2945
Bsp1407I	TGTACA	2 cut(s)	2703, 2764
Bsp143I	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
Bsp1720I	GCTNAGC	1 cut(s)	192
Bsp19I	CCATGG	1 cut(s)	1108
BspACI	CCGC	6 cut(s)	214, 329, 441, 549, 1219, 1634
BspCNI	CTCAG	9 cut(s)	184, 475, 744, 1542, 1672, 1897, 1970, 2086, 2892
BspLI	GGNNCC	3 cut(s)	1073, 1354, 2942
BspMAI	CTGCAG	1 cut(s)	1126
BspMI	ACCTGC	2 cut(s)	554, 2512
BspPI	GGATC	4 cut(s)	517, 1462, 1632, 2453
BspT104I	TTCGAA	1 cut(s)	354
BspT107I	GGYRCC	1 cut(s)	1071
BspTI	CTTAAG	2 cut(s)	2228, 3031
BspTNI	GGTCTC	1 cut(s)	1821
BsrDI	GCAATG	2 cut(s)	253, 1974
BsrFI	RCCGGY	1 cut(s)	187
BsrGI	TGTACA	2 cut(s)	2703, 2764
BsrI	ACTGG	4 cut(s)	642, 1251, 1483, 2365
BssAI	RCCGGY	1 cut(s)	187
BssMI	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
BssNAI	GTATAC	1 cut(s)	1207
BssNI	GRCGYC	2 cut(s)	165, 1835
BssT1I	CCWWGG	4 cut(s)	793, 1093, 1108, 1735
Bst1107I	GTATAC	1 cut(s)	1207
Bst2UI	CCWGG	4 cut(s)	1013, 1233, 2497, 3066
Bst6I	CTCTTC	4 cut(s)	198, 894, 1819, 2610
BstACI	GRCGYC	2 cut(s)	165, 1835
BstAFI	CTTAAG	2 cut(s)	2228, 3031
BstAPI	GCANNNNNTGC	1 cut(s)	19
BstAUI	TGTACA	2 cut(s)	2703, 2764
BstBI	TTCGAA	1 cut(s)	354
BstC8I	GCNNGC	9 cut(s)	189, 790, 1100, 2061, 2268, 2380, 2384, 2481, 2737
BstDSI	CCRYGG	1 cut(s)	1108
BstENI	CCTNNNNNAGG	1 cut(s)	2242
BstF5I	GGATG	1 cut(s)	1008
BstHHI	GCGC	3 cut(s)	228, 2266, 2272
BstKTI	GATC	9 cut(s)	512, 652, 994, 1264, 1457, 1627, 2309, 2461, 2812
BstMAI	GTCTC	2 cut(s)	1821, 1911
BstMBI	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
BstNI	CCWGG	4 cut(s)	1013, 1233, 2497, 3066
BstNSI	RCATGY	3 cut(s)	686, 2483, 2854
BstPAI	GACNNNNGTC	1 cut(s)	1278
BstSCI	CCNGG	7 cut(s)	268, 1011, 1231, 2372, 2495, 2943, 3064
BstSFI	CTRYAG	1 cut(s)	1122
BstV1I	GCAGC	7 cut(s)	149, 1136, 1402, 2155, 2246, 2809, 2872
BstV2I	GAAGAC	2 cut(s)	729, 2322
BstX2I	RGATCY	4 cut(s)	649, 1624, 2306, 2458
BstYI	RGATCY	4 cut(s)	649, 1624, 2306, 2458
BstZ17I	GTATAC	1 cut(s)	1207
Bsu36I	CCTNAGG	1 cut(s)	1680
BtgI	CCRYGG	1 cut(s)	1108
BtsCI	GGATG	1 cut(s)	1008
BtsI	GCAGTG	2 cut(s)	235, 2427
BtsIMutI	CAGTG	6 cut(s)	235, 649, 1537, 1761, 2358, 2427
BveI	ACCTGC	2 cut(s)	554, 2512
Cac8I	GCNNGC	9 cut(s)	189, 790, 1100, 2061, 2268, 2380, 2384, 2481, 2737
CaiI	CAGNNNCTG	2 cut(s)	1396, 3019
CfoI	GCGC	3 cut(s)	228, 2266, 2272
Cfr10I	RCCGGY	1 cut(s)	187
Cfr13I	GGNCC	9 cut(s)	296, 839, 1221, 1248, 1352, 1481, 2672, 3027, 3042
CseI	GACGC	3 cut(s)	154, 2077, 2111
Csp6I	GTAC	9 cut(s)	349, 1072, 1254, 1676, 1811, 2124, 2555, 2704, 2765
CspCI	CAANNNNNGTGG	2 cut(s)	720, 755
CviQI	GTAC	9 cut(s)	349, 1072, 1254, 1676, 1811, 2124, 2555, 2704, 2765
DpnI	GATC	9 cut(s)	511, 651, 993, 1263, 1456, 1626, 2308, 2460, 2811
DpnII	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
DraI	TTTAAA	2 cut(s)	78, 972
DraIII	CACNNNGTG	1 cut(s)	1532
DriI	GACNNNNNGTC	1 cut(s)	1834
EaeI	YGGCCR	4 cut(s)	1444, 2370, 2498, 2787
Eam1104I	CTCTTC	4 cut(s)	198, 894, 1819, 2610
Eam1105I	GACNNNNNGTC	1 cut(s)	1834
EarI	CTCTTC	4 cut(s)	198, 894, 1819, 2610
EciI	GGCGGA	1 cut(s)	203
Eco130I	CCWWGG	4 cut(s)	793, 1093, 1108, 1735
Eco147I	AGGCCT	1 cut(s)	3064
Eco24I	GRGCYC	3 cut(s)	2300, 2380, 2945
Eco31I	GGTCTC	1 cut(s)	1821
Eco32I	GATATC	2 cut(s)	85, 1136
Eco47I	GGWCC	5 cut(s)	1352, 1481, 2672, 3027, 3042
Eco57I	CTGAAG	1 cut(s)	41
Eco81I	CCTNAGG	1 cut(s)	1680
Eco88I	CYCGRG	1 cut(s)	2172
EcoNI	CCTNNNNNAGG	1 cut(s)	2242
EcoO109I	RGGNCCY	2 cut(s)	296, 3027
EcoRII	CCWGG	4 cut(s)	1011, 1231, 2495, 3064
EcoRV	GATATC	2 cut(s)	85, 1136
EcoT14I	CCWWGG	4 cut(s)	793, 1093, 1108, 1735
EcoT22I	ATGCAT	2 cut(s)	1866, 2481
EcoT38I	GRGCYC	3 cut(s)	2300, 2380, 2945
ErhI	CCWWGG	4 cut(s)	793, 1093, 1108, 1735
FalI	AAGNNNNNCTT	8 cut(s)	952, 984, 1312, 1344, 2222, 2254, 2259, 2291
FaqI	GGGAC	2 cut(s)	610, 1338
FauI	CCCGC	1 cut(s)	542
FbaI	TGATCA	2 cut(s)	1261, 2809
FblI	GTMKAC	1 cut(s)	1206
Fnu4HI	GCNGC	9 cut(s)	138, 330, 1125, 1220, 1391, 2169, 2235, 2823, 2861
FokI	GGATG	1 cut(s)	995
FriOI	GRGCYC	3 cut(s)	2300, 2380, 2945
Fsp4HI	GCNGC	9 cut(s)	138, 330, 1125, 1220, 1391, 2169, 2235, 2823, 2861
FspBI	CTAG	7 cut(s)	99, 464, 689, 1094, 1868, 2310, 2952
FspI	TGCGCA	1 cut(s)	227
GlaI	GCGC	3 cut(s)	227, 2265, 2271
GluI	GCNGC	9 cut(s)	138, 330, 1125, 1220, 1391, 2169, 2235, 2823, 2861
GsaI	CCCAGC	1 cut(s)	2328
GsuI	CTGGAG	2 cut(s)	2382, 2583
HapII	CCGG	7 cut(s)	188, 270, 837, 2373, 2660, 2945, 3045
HgaI	GACGC	3 cut(s)	154, 2077, 2111
HhaI	GCGC	3 cut(s)	228, 2266, 2272
Hin1I	GRCGYC	2 cut(s)	165, 1835
Hin6I	GCGC	3 cut(s)	226, 2264, 2270
HinP1I	GCGC	3 cut(s)	226, 2264, 2270
HincII	GTYRAC	3 cut(s)	1873, 1897, 2179
HindII	GTYRAC	3 cut(s)	1873, 1897, 2179
HindIII	AAGCTT	2 cut(s)	906, 1055
HpaI	GTTAAC	1 cut(s)	2179
HpaII	CCGG	7 cut(s)	188, 270, 837, 2373, 2660, 2945, 3045
HphI	GGTGA	4 cut(s)	896, 1921, 2599, 3059
Hpy166II	GTNNAC	9 cut(s)	454, 698, 1207, 1873, 1897, 2024, 2126, 2179, 2590
Hpy8I	GTNNAC	9 cut(s)	454, 698, 1207, 1873, 1897, 2024, 2126, 2179, 2590
Hpy99I	CGWCG	2 cut(s)	10, 170
HpyCH4IV	ACGT	5 cut(s)	830, 983, 1835, 2004, 2485
HpySE526I	ACGT	5 cut(s)	830, 983, 1835, 2004, 2485
Hsp92I	GRCGYC	2 cut(s)	165, 1835
HspAI	GCGC	3 cut(s)	226, 2264, 2270
KpnI	GGTACC	1 cut(s)	1075
KroI	GCCGGC	1 cut(s)	187
KroNI	GCCGGC	1 cut(s)	189
Ksp22I	TGATCA	2 cut(s)	1261, 2809
KspAI	GTTAAC	1 cut(s)	2179
Kzo9I	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
LmnI	GCTCC	5 cut(s)	10, 272, 527, 2261, 2940
Lsp1109I	GCAGC	7 cut(s)	149, 1136, 1402, 2155, 2246, 2809, 2872
LweI	GCATC	6 cut(s)	125, 787, 1584, 1873, 2466, 2809
MaeI	CTAG	7 cut(s)	99, 464, 689, 1094, 1868, 2310, 2952
MaeII	ACGT	5 cut(s)	830, 983, 1835, 2004, 2485
MaeIII	GTNAC	4 cut(s)	302, 1418, 1574, 1909
MalI	GATC	9 cut(s)	511, 651, 993, 1263, 1456, 1626, 2308, 2460, 2811
MboI	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
MfeI	CAATTG	1 cut(s)	504
MflI	RGATCY	4 cut(s)	649, 1624, 2306, 2458
MhlI	GDGCHC	4 cut(s)	1528, 2300, 2380, 2945
MlsI	TGGCCA	3 cut(s)	1446, 2500, 2789
MluNI	TGGCCA	3 cut(s)	1446, 2500, 2789
MlyI	GAGTC	4 cut(s)	79, 299, 2039, 2782
MmeI	TCCRAC	2 cut(s)	425, 2756
Mox20I	TGGCCA	3 cut(s)	1446, 2500, 2789
Mph1103I	ATGCAT	2 cut(s)	1866, 2481
MroNI	GCCGGC	1 cut(s)	187
MroXI	GAANNNNTTC	4 cut(s)	51, 557, 571, 1328
MscI	TGGCCA	3 cut(s)	1446, 2500, 2789
MslI	CAYNNNNRTG	4 cut(s)	1107, 1269, 1769, 2601
Msp20I	TGGCCA	3 cut(s)	1446, 2500, 2789
MspCI	CTTAAG	2 cut(s)	2228, 3031
MspI	CCGG	7 cut(s)	188, 270, 837, 2373, 2660, 2945, 3045
MspR9I	CCNGG	7 cut(s)	270, 1013, 1233, 2374, 2497, 2945, 3066
MssI	GTTTAAAC	1 cut(s)	78
MunI	CAATTG	1 cut(s)	504
Mva1269I	GAATGC	2 cut(s)	53, 2886
MvaI	CCWGG	4 cut(s)	1013, 1233, 2497, 3066
NaeI	GCCGGC	1 cut(s)	189
NciI	CCSGG	3 cut(s)	270, 2374, 2945
NcoI	CCATGG	1 cut(s)	1108
NdeII	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
NgoMIV	GCCGGC	1 cut(s)	187
NlaIV	GGNNCC	3 cut(s)	1073, 1354, 2942
NmeAIII	GCCGAG	1 cut(s)	133
NmuCI	GTSAC	2 cut(s)	302, 1909
NsbI	TGCGCA	1 cut(s)	227
NsiI	ATGCAT	2 cut(s)	1866, 2481
NspI	RCATGY	3 cut(s)	686, 2483, 2854
NspV	TTCGAA	1 cut(s)	354
PaeI	GCATGC	1 cut(s)	2483
PaeR7I	CTCGAG	1 cut(s)	2172
PaqCI	CACCTGC	1 cut(s)	2512
PceI	AGGCCT	1 cut(s)	3064
PciI	ACATGT	1 cut(s)	682
PctI	GAATGC	2 cut(s)	53, 2886
PdiI	GCCGGC	1 cut(s)	189
PdmI	GAANNNNTTC	4 cut(s)	51, 557, 571, 1328
PfeI	GAWTC	8 cut(s)	1399, 1747, 2313, 2537, 2549, 2620, 2801, 2962
PflFI	GACNNNGTC	1 cut(s)	832
PflMI	CCANNNNNTGG	2 cut(s)	1114, 3002
PfoI	TCCNGGA	1 cut(s)	1011
PkrI	GCNGC	9 cut(s)	139, 331, 1126, 1221, 1392, 2170, 2236, 2824, 2862
PleI	GAGTC	4 cut(s)	78, 299, 2039, 2781
PmeI	GTTTAAAC	1 cut(s)	78
PpsI	GAGTC	4 cut(s)	78, 299, 2039, 2781
PpuMI	RGGWCCY	1 cut(s)	3027
PscI	ACATGT	1 cut(s)	682
PshAI	GACNNNNGTC	1 cut(s)	1278
PshBI	ATTAAT	1 cut(s)	1671
Psp1406I	AACGTT	1 cut(s)	983
Psp5II	RGGWCCY	1 cut(s)	3027
Psp6I	CCWGG	4 cut(s)	1011, 1231, 2495, 3064
PspFI	CCCAGC	1 cut(s)	2324
PspGI	CCWGG	4 cut(s)	1011, 1231, 2495, 3064
PspN4I	GGNNCC	3 cut(s)	1073, 1354, 2942
PspPI	GGNCC	9 cut(s)	296, 839, 1221, 1248, 1352, 1481, 2672, 3027, 3042
PspPPI	RGGWCCY	1 cut(s)	3027
PspXI	VCTCGAGB	1 cut(s)	2172
PstI	CTGCAG	1 cut(s)	1126
PstNI	CAGNNNCTG	2 cut(s)	1396, 3019
PsuI	RGATCY	4 cut(s)	649, 1624, 2306, 2458
PsyI	GACNNNGTC	1 cut(s)	832
RsaI	GTAC	9 cut(s)	350, 1073, 1255, 1677, 1812, 2125, 2556, 2705, 2766
RsaNI	GTAC	9 cut(s)	349, 1072, 1254, 1676, 1811, 2124, 2555, 2704, 2765
RseI	CAYNNNNRTG	4 cut(s)	1107, 1269, 1769, 2601
SatI	GCNGC	9 cut(s)	138, 330, 1125, 1220, 1391, 2169, 2235, 2823, 2861
Sau3AI	GATC	9 cut(s)	509, 649, 991, 1261, 1454, 1624, 2306, 2458, 2809
Sau96I	GGNCC	9 cut(s)	296, 839, 1221, 1248, 1352, 1481, 2672, 3027, 3042
ScaI	AGTACT	3 cut(s)	350, 1255, 2556
SchI	GAGTC	4 cut(s)	79, 299, 2039, 2782
ScrFI	CCNGG	7 cut(s)	270, 1013, 1233, 2374, 2497, 2945, 3066
SduI	GDGCHC	4 cut(s)	1528, 2300, 2380, 2945
SfaNI	GCATC	6 cut(s)	125, 787, 1584, 1873, 2466, 2809
SfcI	CTRYAG	1 cut(s)	1122
Sfr274I	CTCGAG	1 cut(s)	2172
SfuI	TTCGAA	1 cut(s)	354
SinI	GGWCC	5 cut(s)	1352, 1481, 2672, 3027, 3042
SlaI	CTCGAG	1 cut(s)	2172
SmiMI	CAYNNNNRTG	4 cut(s)	1107, 1269, 1769, 2601
SmlI	CTYRAG	4 cut(s)	2172, 2228, 2463, 3031
SmoI	CTYRAG	4 cut(s)	2172, 2228, 2463, 3031
SphI	GCATGC	1 cut(s)	2483
SseBI	AGGCCT	1 cut(s)	3064
SsiI	CCGC	6 cut(s)	214, 329, 441, 549, 1219, 1634
SspI	AATATT	2 cut(s)	521, 2282
SspMI	CTAG	7 cut(s)	99, 464, 689, 1094, 1868, 2310, 2952
StuI	AGGCCT	1 cut(s)	3064
StyD4I	CCNGG	7 cut(s)	268, 1011, 1231, 2372, 2495, 2943, 3064
StyI	CCWWGG	4 cut(s)	793, 1093, 1108, 1735
TaiI	ACGT	5 cut(s)	833, 986, 1838, 2007, 2488
TaqII	GACCGA	3 cut(s)	1392, 1498, 2689
TatI	WGTACW	7 cut(s)	348, 1253, 1810, 2123, 2554, 2703, 2764
TauI	GCSGC	2 cut(s)	332, 1222
TfiI	GAWTC	8 cut(s)	1399, 1747, 2313, 2537, 2549, 2620, 2801, 2962
TscAI	CASTG	6 cut(s)	235, 649, 1537, 1761, 2365, 2434
TseFI	GTSAC	2 cut(s)	302, 1909
TseI	GCWGC	7 cut(s)	137, 1124, 1390, 2168, 2234, 2822, 2860
Tsp45I	GTSAC	2 cut(s)	302, 1909
TspDTI	ATGAA	9 cut(s)	147, 672, 821, 905, 1067, 1745, 1787, 2072, 2613
TspGWI	ACGGA	6 cut(s)	23, 298, 683, 1680, 1836, 3004
TspRI	CASTG	6 cut(s)	235, 649, 1537, 1761, 2365, 2434
Tth111I	GACNNNGTC	1 cut(s)	832
Van91I	CCANNNNNTGG	2 cut(s)	1114, 3002
Vha464I	CTTAAG	2 cut(s)	2228, 3031
VpaK11BI	GGWCC	5 cut(s)	1352, 1481, 2672, 3027, 3042
VspI	ATTAAT	1 cut(s)	1671
XagI	CCTNNNNNAGG	1 cut(s)	2242
XapI	RAATTY	7 cut(s)	571, 589, 1581, 1602, 1644, 2132, 2524
XbaI	TCTAGA	1 cut(s)	2309
XceI	RCATGY	3 cut(s)	686, 2483, 2854
XcmI	CCANNNNNNNNNTGG	2 cut(s)	37, 2853
XhoI	CTCGAG	1 cut(s)	2172
XmaJI	CCTAGG	1 cut(s)	1093
XmiI	GTMKAC	1 cut(s)	1206
XmnI	GAANNNNTTC	4 cut(s)	51, 557, 571, 1328
XspI	CTAG	7 cut(s)	99, 464, 689, 1094, 1868, 2310, 2952
ZraI	GACGTC	1 cut(s)	1836
ZrmI	AGTACT	3 cut(s)	350, 1255, 2556
Zsp2I	ATGCAT	2 cut(s)	1866, 2481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.