FvH4_3g12180

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001185
Physical Location & Seq
Forward (+)
7209648 .. 7211006
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12180.t1

Sequence Viewer

Length: 861 bp
ATGGGGACAGGGTGGTCACAAGATGAGGCCTCACAGTCACAGCAGCCGAGTCCTGCAGAACCAACTCAACCAAGTAACAAGGCAGTAGTGGAGAAAGTAACAGAAGCAACGCCTAGCGGCTCTAAACCAATGGCAAAGGTGGAGAAATCTGCTAGTTTTCAGGCCAAACCTTCAGCTGATCACAAGCAGGAGACCAAAGTAGTGAAAGAAGTCAAGGGAACAGAGAAACCGACAGTTACACAAGTGAACCTGCAGCCGCAGCTCCCACATAATTACGAAGCTATTGTAAAAGATGCCGACACTCCCATCAACAAGTCACCGGAACACCTGTTTGGACATCTTTATGCTGGAATACTCTTAAACCAAAAGAGGAAGAAGTATTGGGTTGACAAGAAGTCCAACAACTGCTTCATGGTGTATCCAAGGGATCTAGCAATCACTTGGGCTGAAGACAATCGTTACTGGCACTGGCCTTCCCTGGAAGAAACAAGTAATGTCTTCATCGATGCAGCTGAACTGTTAAATGTATGCTGGCTTGAAGTTCATGGAAAAATTGATTCCACAAAGCTGTCACCAGGAACTCTATACGAAGTAGTATTTATAGTGATGTTAAAAGCTGCAGCTTATGGATGGAAAGTTCCTGTCAATGTCAGACTCACTCTCCCAGATGGTAACAAGAAAGAAAGTAAAGCTGATCTGAATAAAATCCCAAGAGAAAAATGGACAGAGATACCAGTAGGCGAGTTCAGAGCATCACCAGGATTACTTGGGAACATTGAATTTTCGATGTATGAGTATGAAGATGGGAAATGGAAGAGTGGACTTCTGATCAAGGGTGTTGCAGTTCGGCCTAAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

287

Amino Acids

32.12

Weight (kDa)

8.59

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 135 - 284 1.4e-44 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 258
AciI CCGC 2 cut(s) 117, 257
AclWI GGATC 1 cut(s) 435
AcsI RAATTY 1 cut(s) 779
AcuI CTGAAG 2 cut(s) 156, 468
AgsI TTSAA 2 cut(s) 539, 779
AhdI GACNNNNNGTC 1 cut(s) 394
AjnI CCWGG 3 cut(s) 477, 574, 757
AjuI GAANNNNNNNTTGG 4 cut(s) 315, 347, 392, 424
AloI GAACNNNNNNTCC 2 cut(s) 621, 653
AluBI AGCT 8 cut(s) 176, 262, 281, 512, 568, 617, 623, 692
AluI AGCT 8 cut(s) 176, 262, 281, 512, 568, 617, 623, 692
Alw26I GTCTC 1 cut(s) 185
AlwI GGATC 1 cut(s) 435
AoxI GGCC 4 cut(s) 27, 162, 470, 848
ApeKI GCWGC 6 cut(s) 43, 253, 259, 509, 617, 620
ApoI RAATTY 1 cut(s) 779
AsuHPI GGTGA 3 cut(s) 309, 564, 747
BbsI GAAGAC 2 cut(s) 456, 490
BbvI GCAGC 6 cut(s) 55, 265, 271, 521, 604, 632
BccI CCATC 4 cut(s) 314, 624, 662, 797
BciT130I CCWGG 3 cut(s) 479, 576, 759
BciVI GTATCC 1 cut(s) 429
BclI TGATCA 2 cut(s) 178, 828
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 3 cut(s) 114, 153, 431
BfmI CTRYAG 3 cut(s) 54, 251, 618
BfuAI ACCTGC 1 cut(s) 258
BfuI GTATCC 1 cut(s) 429
BisI GCNGC 8 cut(s) 44, 118, 254, 257, 260, 510, 618, 621
BlsI GCNGC 8 cut(s) 45, 119, 255, 258, 261, 511, 619, 622
Bme1390I CCNGG 3 cut(s) 479, 576, 759
BmeRI GACNNNNNGTC 1 cut(s) 394
BmrFI CCNGG 3 cut(s) 479, 576, 759
BmsI GCATC 3 cut(s) 283, 496, 761
BpiI GAAGAC 2 cut(s) 456, 490
Bsa29I ATCGAT 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 2 cut(s) 422, 477
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bse1I ACTGG 3 cut(s) 467, 473, 734
BseBI CCWGG 3 cut(s) 479, 576, 759
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 422, 477
BseGI GGATG 1 cut(s) 635
BseNI ACTGG 3 cut(s) 467, 473, 734
BseXI GCAGC 6 cut(s) 55, 265, 271, 521, 604, 632
BshFI GGCC 4 cut(s) 29, 164, 472, 850
BshVI ATCGAT 1 cut(s) 504
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 4 cut(s) 29, 164, 472, 850
Bso31I GGTCTC 1 cut(s) 185
Bsp143I GATC 4 cut(s) 178, 427, 694, 828
BspACI CCGC 2 cut(s) 117, 257
BspANI GGCC 4 cut(s) 29, 164, 472, 850
BspDI ATCGAT 1 cut(s) 504
BspMAI CTGCAG 3 cut(s) 58, 255, 622
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 1 cut(s) 435
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 3 cut(s) 467, 473, 734
BssECI CCNNGG 2 cut(s) 422, 477
BssMI GATC 4 cut(s) 178, 427, 694, 828
BssT1I CCWWGG 1 cut(s) 422
Bst2UI CCWGG 3 cut(s) 479, 576, 759
Bst4CI ACNGT 3 cut(s) 36, 235, 519
Bst6I CTCTTC 1 cut(s) 809
BstC8I GCNNGC 1 cut(s) 533
BstF5I GGATG 1 cut(s) 635
BstKTI GATC 4 cut(s) 181, 430, 697, 831
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 4 cut(s) 178, 427, 694, 828
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 3 cut(s) 479, 576, 759
BstSCI CCNGG 3 cut(s) 477, 574, 757
BstSFI CTRYAG 3 cut(s) 54, 251, 618
BstV1I GCAGC 6 cut(s) 55, 265, 271, 521, 604, 632
BstV2I GAAGAC 2 cut(s) 456, 490
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 504
BsuI GTATCC 1 cut(s) 429
BsuRI GGCC 4 cut(s) 29, 164, 472, 850
BsuTUI ATCGAT 1 cut(s) 504
BtsCI GGATG 1 cut(s) 635
BtsIMutI CAGTG 1 cut(s) 466
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 533
ClaI ATCGAT 1 cut(s) 504
CviAII CATG 2 cut(s) 412, 545
DpnI GATC 4 cut(s) 180, 429, 696, 830
DpnII GATC 4 cut(s) 178, 427, 694, 828
DrdI GACNNNNNNGTC 1 cut(s) 13
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 809
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 809
Eco130I CCWWGG 1 cut(s) 422
Eco147I AGGCCT 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 156, 468
EcoRII CCWGG 3 cut(s) 477, 574, 757
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 2 cut(s) 415, 548
FaqI GGGAC 1 cut(s) 19
FatI CATG 2 cut(s) 411, 544
FbaI TGATCA 2 cut(s) 178, 828
Fnu4HI GCNGC 8 cut(s) 44, 118, 254, 257, 260, 510, 618, 621
FokI GGATG 1 cut(s) 642
Fsp4HI GCNGC 8 cut(s) 44, 118, 254, 257, 260, 510, 618, 621
FspBI CTAG 3 cut(s) 114, 153, 431
GluI GCNGC 8 cut(s) 44, 118, 254, 257, 260, 510, 618, 621
HaeIII GGCC 4 cut(s) 29, 164, 472, 850
HapII CCGG 1 cut(s) 320
Hin1II CATG 2 cut(s) 415, 548
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 3 cut(s) 49, 557, 654
HpaII CCGG 1 cut(s) 320
HphI GGTGA 3 cut(s) 309, 564, 747
Hpy166II GTNNAC 3 cut(s) 247, 388, 821
Hpy188I TCNGA 4 cut(s) 653, 699, 749, 828
Hpy8I GTNNAC 3 cut(s) 247, 388, 821
HpyAV CCTTC 2 cut(s) 180, 483
HpyCH4III ACNGT 3 cut(s) 36, 235, 519
HpyCH4V TGCA 5 cut(s) 56, 253, 509, 620, 842
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
Hsp92II CATG 2 cut(s) 415, 548
Ksp22I TGATCA 2 cut(s) 178, 828
Kzo9I GATC 4 cut(s) 178, 427, 694, 828
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 6 cut(s) 55, 265, 271, 521, 604, 632
LweI GCATC 3 cut(s) 283, 496, 761
MaeI CTAG 3 cut(s) 114, 153, 431
MaeIII GTNAC 9 cut(s) 15, 36, 74, 97, 235, 315, 458, 570, 671
MalI GATC 4 cut(s) 180, 429, 696, 830
MboI GATC 4 cut(s) 178, 427, 694, 828
MboII GAAGA 6 cut(s) 385, 461, 490, 494, 812, 826
MflI RGATCY 1 cut(s) 427
MluCI AATT 3 cut(s) 271, 552, 779
MlyI GAGTC 2 cut(s) 58, 648
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 3 cut(s) 19, 40, 363
MseI TTAA 3 cut(s) 359, 521, 611
MslI CAYNNNNRTG 1 cut(s) 342
MspA1I CMGCKG 2 cut(s) 176, 512
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 3 cut(s) 479, 576, 759
MvaI CCWGG 3 cut(s) 479, 576, 759
MwoI GCNNNNNNNGC 1 cut(s) 259
NdeII GATC 4 cut(s) 178, 427, 694, 828
NlaIII CATG 2 cut(s) 415, 548
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 4 cut(s) 15, 36, 315, 570
PceI AGGCCT 1 cut(s) 29
PfeI GAWTC 1 cut(s) 557
PkrI GCNGC 8 cut(s) 45, 119, 255, 258, 261, 511, 619, 622
PleI GAGTC 2 cut(s) 57, 648
PpsI GAGTC 2 cut(s) 57, 648
Psp6I CCWGG 3 cut(s) 477, 574, 757
PspGI CCWGG 3 cut(s) 477, 574, 757
PstI CTGCAG 3 cut(s) 58, 255, 622
PsuI RGATCY 1 cut(s) 427
PvuII CAGCTG 2 cut(s) 176, 512
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 3 cut(s) 359, 521, 611
SatI GCNGC 8 cut(s) 44, 118, 254, 257, 260, 510, 618, 621
Sau3AI GATC 4 cut(s) 178, 427, 694, 828
SchI GAGTC 2 cut(s) 58, 648
ScrFI CCNGG 3 cut(s) 479, 576, 759
SfaNI GCATC 3 cut(s) 283, 496, 761
SfcI CTRYAG 3 cut(s) 54, 251, 618
SmiMI CAYNNNNRTG 1 cut(s) 342
Sse9I AATT 3 cut(s) 271, 552, 779
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 2 cut(s) 117, 257
SspMI CTAG 3 cut(s) 114, 153, 431
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 477, 574, 757
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 3 cut(s) 36, 235, 519
TaqI TCGA 2 cut(s) 504, 785
TasI AATT 3 cut(s) 271, 552, 779
TauI GCSGC 2 cut(s) 120, 259
TfiI GAWTC 1 cut(s) 557
Tru1I TTAA 3 cut(s) 359, 521, 611
Tru9I TTAA 3 cut(s) 359, 521, 611
TscAI CASTG 1 cut(s) 473
TseFI GTSAC 4 cut(s) 15, 36, 315, 570
TseI GCWGC 6 cut(s) 43, 253, 259, 509, 617, 620
Tsp45I GTSAC 4 cut(s) 15, 36, 315, 570
TspDTI ATGAA 4 cut(s) 400, 490, 533, 813
TspRI CASTG 1 cut(s) 473
XapI RAATTY 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XspI CTAG 3 cut(s) 114, 153, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.