MD05G1253400.v1.1

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001185
Physical Location & Seq
Reverse (-)
38446929 .. 38448134
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1253400.v1.1.491

Sequence Viewer

Length: 495 bp
ATGAAAAAGAAATATTGGGTTGATAAGAATTCCAACAACTGTTTTACGGTGTATGCAAGAGATCTCTTGATCACTTGGGGCGGAGATGGTCGTTACTGGCGCTGGAACACCGTAGAAGACGGTGATGACCGCATTGATGCTGCTGAACTGTTAGATGCATGTTGGCTAGAAGTGCACGGGAAGTTTGAAACCGCATACCTGTCACCCGGAACTCTCTATGAAGTTGTGTTTGTGGTCAAGCTGAATGCTAAAGAATATGGATGGGAAGAACCAGTCGAAATCACTCTCGAAACCCCTGCTGGTGACAAGCACACTCGTAAGGTTGATCTGATGCAAAAGCAGAGAGGAAAATGGGTTGAGATCCCAGCCGGCGAATTTAGAGCATCGCCTGAGCAGTATGGCTACATGATTTTTTCACTCCGTCAACTTAGTGGGCAATGGAAGAAAGGGTTTGTTATCAAGGGTGCCGAAATTCGTCCTAAAACTAATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

165

Amino Acids

19.09

Weight (kDa)

7.7

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 13 - 160 4.9e-40 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AciI CCGC 3 cut(s) 81, 130, 192
AclWI GGATC 1 cut(s) 355
AcsI RAATTY 3 cut(s) 28, 374, 471
AgsI TTSAA 1 cut(s) 188
AluBI AGCT 1 cut(s) 241
AluI AGCT 1 cut(s) 241
Alw21I GWGCWC 1 cut(s) 177
Alw44I GTGCAC 1 cut(s) 173
AlwI GGATC 1 cut(s) 355
ApaLI GTGCAC 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 3 cut(s) 28, 374, 471
AspLEI GCGC 1 cut(s) 102
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 3 cut(s) 134, 195, 314
BaeGI GKGCMC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 464
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 80, 255
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 1 cut(s) 207
BfaI CTAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 103
BglII AGATCT 1 cut(s) 61
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 4 cut(s) 127, 145, 321, 392
BpiI GAAGAC 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 390
BpuMI CCSGG 1 cut(s) 207
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 2 cut(s) 101, 272
Bse3DI GCAATG 1 cut(s) 443
BseGI GGATG 1 cut(s) 266
BseMI GCAATG 1 cut(s) 443
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 101, 272
BseSI GKGCMC 1 cut(s) 177
BseXI GCAGC 1 cut(s) 127
BseYI CCCAGC 1 cut(s) 364
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 177
BsiSI CCGG 2 cut(s) 207, 369
BsmI GAATGC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 4 cut(s) 61, 69, 325, 360
BspACI CCGC 3 cut(s) 81, 130, 192
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 466
BspPI GGATC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 464
BsrDI GCAATG 1 cut(s) 443
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 2 cut(s) 101, 272
BssAI RCCGGY 1 cut(s) 368
BssMI GATC 4 cut(s) 61, 69, 325, 360
Bst4CI ACNGT 5 cut(s) 41, 49, 112, 122, 150
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 390, 428
BstF5I GGATG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 1 cut(s) 102
BstKTI GATC 4 cut(s) 64, 72, 328, 363
BstMBI GATC 4 cut(s) 61, 69, 325, 360
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 61, 360
BstYI RGATCY 2 cut(s) 61, 360
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 370
CfoI GCGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 368
CviAII CATG 2 cut(s) 159, 406
CviJI RGCY 4 cut(s) 166, 241, 368, 402
CviKI_1 RGCY 4 cut(s) 166, 241, 368, 402
DdeI CTNAG 2 cut(s) 390, 428
DpnI GATC 4 cut(s) 63, 71, 327, 362
DpnII GATC 4 cut(s) 61, 69, 325, 360
EciI GGCGGA 1 cut(s) 96
EcoRI GAATTC 1 cut(s) 28
EcoT22I ATGCAT 1 cut(s) 160
FaeI CATG 2 cut(s) 162, 409
FaiI YATR 7 cut(s) 54, 160, 196, 219, 258, 399, 407
FatI CATG 2 cut(s) 158, 405
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 273
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 167
GlaI GCGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 141
GsaI CCCAGC 1 cut(s) 368
HaeII RGCGCY 1 cut(s) 103
HapII CCGG 2 cut(s) 207, 369
HhaI GCGC 1 cut(s) 102
Hin1II CATG 2 cut(s) 162, 409
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HincII GTYRAC 1 cut(s) 425
HindII GTYRAC 1 cut(s) 425
HpaII CCGG 2 cut(s) 207, 369
HphI GGTGA 3 cut(s) 134, 195, 314
Hpy166II GTNNAC 2 cut(s) 175, 425
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 2 cut(s) 67, 287
Hpy8I GTNNAC 2 cut(s) 175, 425
HpyCH4III ACNGT 5 cut(s) 41, 49, 112, 122, 150
HpyCH4V TGCA 4 cut(s) 56, 158, 175, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 2 cut(s) 390, 428
Hsp92II CATG 2 cut(s) 162, 409
HspAI GCGC 1 cut(s) 100
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 61, 69, 325, 360
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 4 cut(s) 127, 145, 321, 392
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 3 cut(s) 92, 201, 302
MalI GATC 4 cut(s) 63, 71, 327, 362
MboI GATC 4 cut(s) 61, 69, 325, 360
MboII GAAGA 3 cut(s) 128, 278, 454
MflI RGATCY 2 cut(s) 61, 360
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 3 cut(s) 28, 374, 471
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 1 cut(s) 338
Mph1103I ATGCAT 1 cut(s) 160
MroNI GCCGGC 1 cut(s) 368
MspI CCGG 2 cut(s) 207, 369
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 172
NaeI GCCGGC 1 cut(s) 370
NciI CCSGG 1 cut(s) 207
NdeII GATC 4 cut(s) 61, 69, 325, 360
NgoMIV GCCGGC 1 cut(s) 368
NlaIII CATG 2 cut(s) 162, 409
NlaIV GGNNCC 1 cut(s) 466
NmuCI GTSAC 2 cut(s) 201, 302
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 162
PctI GAATGC 1 cut(s) 250
PdiI GCCGGC 1 cut(s) 370
PkrI GCNGC 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 466
PsuI RGATCY 2 cut(s) 61, 360
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 4 cut(s) 61, 69, 325, 360
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 177
SetI ASST 3 cut(s) 201, 243, 324
SfaNI GCATC 4 cut(s) 127, 145, 321, 392
Sse9I AATT 3 cut(s) 28, 374, 471
SsiI CCGC 3 cut(s) 81, 130, 192
SspI AATATT 1 cut(s) 14
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 5 cut(s) 41, 49, 112, 122, 150
TaqI TCGA 2 cut(s) 276, 288
TasI AATT 3 cut(s) 28, 374, 471
TseFI GTSAC 2 cut(s) 201, 302
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 2 cut(s) 201, 302
TspDTI ATGAA 2 cut(s) 17, 234
TspGWI ACGGA 1 cut(s) 410
VneI GTGCAC 1 cut(s) 173
XapI RAATTY 3 cut(s) 28, 374, 471
XceI RCATGY 1 cut(s) 162
XspI CTAG 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.