Prupe.8G030100_v2.0.a1

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001185
Physical Location & Seq
Forward (+)
2807215 .. 2809814
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G030100.2

Sequence Viewer

Length: 648 bp
ATGCACAGGAGAAAATATATAGTGGACAAACAACTAGTAACAATGCATCGGAGAAACCAACCGAAGGAGGGCCTTCTTCTACTGCAGCTCCCTCTGATAATCAGAAGGTACGTAGAAGTCAAAAACGCTTCTGATAATGAGAAGACAGAAACACAATCACCTGTGCAGACGTATGGCGATGGAAAGTCCAAATGGTGTATGGTGTATGCAAAGGCTCTCGATATCACTTGGTCTGGAGACACTCGTTACTGGCGATGGGCCAAAGAAACTAGTGGTGACAACTTTGAAGCTGCTGAACTGTTAAATGTGTGTTGGCTAGAAGTGCGTGGAAAACTTGCGACCACAGACCTCTCACCAGGAACTCTGTATGAAGTTGTATTTGTGGTCAAGATGAAAACTGAAGCTGATGGATGGGATGCTCCAGTGAATTTCAAATTCACTTCCCCAGATGGTGCATTAACAAGGAATACGACATTTAAATTGAGGGAGATTTTGAAGGATAGTAAAGATCAATGGAAAGATATTCCGTTTGGTGAGTTTAAAGCACCCGAAAATCCTAGGAATATCGAGTTTTCGCTGTATGAATATAGTAGGATATGGAAGAGCGGACTTGTTATCAAGGGTGTTGCCGTTCGACCAAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

216

Amino Acids

24.93

Weight (kDa)

9.28

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 606
AciI CCGC 1 cut(s) 606
AcsI RAATTY 2 cut(s) 427, 434
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 64, 68
AgsI TTSAA 3 cut(s) 287, 433, 496
AhlI ACTAGT 2 cut(s) 34, 269
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 3 cut(s) 88, 290, 404
AluI AGCT 3 cut(s) 88, 290, 404
Alw26I GTCTC 1 cut(s) 231
AoxI GGCC 2 cut(s) 70, 258
ApeKI GCWGC 2 cut(s) 85, 290
ApoI RAATTY 2 cut(s) 427, 434
AspA2I CCTAGG 1 cut(s) 557
AspS9I GGNCC 2 cut(s) 70, 258
AsuHPI GGTGA 4 cut(s) 150, 287, 345, 545
AvrII CCTAGG 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 149
BbvI GCAGC 2 cut(s) 97, 277
BccI CCATC 5 cut(s) 173, 249, 401, 405, 443
BceAI ACGGC 1 cut(s) 614
BciT130I CCWGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 231
BcuI ACTAGT 2 cut(s) 34, 269
BfaI CTAG 4 cut(s) 35, 270, 317, 558
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 86, 291
BlnI CCTAGG 1 cut(s) 557
BlsI GCNGC 2 cut(s) 87, 292
Bme1390I CCNGG 1 cut(s) 357
BmgT120I GGNCC 2 cut(s) 70, 258
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 2 cut(s) 55, 406
BpiI GAAGAC 1 cut(s) 149
BpmI CTGGAG 2 cut(s) 255, 405
BsaAI YACGTR 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 68
Bse1I ACTGG 2 cut(s) 254, 422
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 2 cut(s) 416, 421
BseLI CCNNNNNNNGG 2 cut(s) 64, 68
BseNI ACTGG 2 cut(s) 254, 422
BseXI GCAGC 2 cut(s) 97, 277
BsgI GTGCAG 1 cut(s) 185
BshFI GGCC 2 cut(s) 72, 260
BslI CCNNNNNNNGG 2 cut(s) 64, 68
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 2 cut(s) 72, 260
Bsp143I GATC 1 cut(s) 508
BspACI CCGC 1 cut(s) 606
BspANI GGCC 2 cut(s) 72, 260
BspMAI CTGCAG 1 cut(s) 87
BspQI GCTCTTC 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 606
BsrI ACTGG 2 cut(s) 254, 422
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 1 cut(s) 508
BssT1I CCWWGG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 300
Bst6I CTCTTC 1 cut(s) 596
BstBAI YACGTR 1 cut(s) 112
BstF5I GGATG 2 cut(s) 416, 421
BstKTI GATC 1 cut(s) 511
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 1 cut(s) 508
BstMWI GCNNNNNNNGC 1 cut(s) 322
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 1 cut(s) 83
BstSNI TACGTA 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 97, 277
BstV2I GAAGAC 1 cut(s) 149
BsuRI GGCC 2 cut(s) 72, 260
BtgZI GCGATG 2 cut(s) 192, 268
BtsCI GGATG 2 cut(s) 416, 421
BtsIMutI CAGTG 1 cut(s) 429
Cfr13I GGNCC 2 cut(s) 70, 258
Csp6I GTAC 1 cut(s) 109
CviJI RGCY 7 cut(s) 72, 88, 215, 260, 290, 316, 404
CviKI_1 RGCY 7 cut(s) 72, 88, 215, 260, 290, 316, 404
CviQI GTAC 1 cut(s) 109
DpnI GATC 1 cut(s) 510
DpnII GATC 1 cut(s) 508
DraI TTTAAA 2 cut(s) 478, 541
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco105I TACGTA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 557
Eco32I GATATC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 355
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 557
EcoT22I ATGCAT 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 557
FaiI YATR 9 cut(s) 18, 20, 174, 200, 207, 369, 582, 588, 598
Fnu4HI GCNGC 2 cut(s) 86, 291
FokI GGATG 2 cut(s) 423, 428
Fsp4HI GCNGC 2 cut(s) 86, 291
FspBI CTAG 4 cut(s) 35, 270, 317, 558
GluI GCNGC 2 cut(s) 86, 291
GsuI CTGGAG 2 cut(s) 255, 405
HaeIII GGCC 2 cut(s) 72, 260
HphI GGTGA 4 cut(s) 150, 287, 345, 545
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 4 cut(s) 51, 96, 104, 133
Hpy188III TCNNGA 3 cut(s) 218, 234, 388
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 4 cut(s) 58, 83, 99, 490
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4IV ACGT 2 cut(s) 111, 170
HpyCH4V TGCA 6 cut(s) 4, 46, 85, 166, 209, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 322
HpySE526I ACGT 2 cut(s) 111, 170
Kzo9I GATC 1 cut(s) 508
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 2 cut(s) 93, 424
LpnPI CCDG 7 cut(s) 174, 219, 235, 342, 369, 435, 459
Lsp1109I GCAGC 2 cut(s) 97, 277
LweI GCATC 2 cut(s) 55, 406
MaeI CTAG 4 cut(s) 35, 270, 317, 558
MaeII ACGT 2 cut(s) 111, 170
MaeIII GTNAC 3 cut(s) 37, 245, 275
MalI GATC 1 cut(s) 510
MbiI CCGCTC 1 cut(s) 606
MboI GATC 1 cut(s) 508
MboII GAAGA 3 cut(s) 68, 154, 613
MluCI AATT 3 cut(s) 427, 434, 479
MnlI CCTC 4 cut(s) 61, 102, 359, 477
Mph1103I ATGCAT 1 cut(s) 48
MseI TTAA 5 cut(s) 302, 458, 477, 540, 646
MspR9I CCNGG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 322
NdeII GATC 1 cut(s) 508
NmuCI GTSAC 1 cut(s) 275
NsiI ATGCAT 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 250
PkrI GCNGC 2 cut(s) 87, 292
Ppu21I YACGTR 1 cut(s) 112
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspPI GGNCC 2 cut(s) 70, 258
PstI CTGCAG 1 cut(s) 87
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 596
SaqAI TTAA 5 cut(s) 302, 458, 477, 540, 646
SatI GCNGC 2 cut(s) 86, 291
Sau3AI GATC 1 cut(s) 508
Sau96I GGNCC 2 cut(s) 70, 258
ScrFI CCNGG 1 cut(s) 357
SetI ASST 8 cut(s) 90, 110, 114, 163, 173, 292, 351, 406
SfaNI GCATC 2 cut(s) 55, 406
SfcI CTRYAG 1 cut(s) 83
SmiI ATTTAAAT 1 cut(s) 478
SnaBI TACGTA 1 cut(s) 112
SpeI ACTAGT 2 cut(s) 34, 269
Sse9I AATT 3 cut(s) 427, 434, 479
SsiI CCGC 1 cut(s) 606
SspMI CTAG 4 cut(s) 35, 270, 317, 558
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 557
SwaI ATTTAAAT 1 cut(s) 478
TaaI ACNGT 1 cut(s) 300
TaiI ACGT 2 cut(s) 114, 173
TaqI TCGA 3 cut(s) 219, 567, 634
TasI AATT 3 cut(s) 427, 434, 479
Tru1I TTAA 5 cut(s) 302, 458, 477, 540, 646
Tru9I TTAA 5 cut(s) 302, 458, 477, 540, 646
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 85, 290
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 384, 407, 597
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 1 cut(s) 429
XapI RAATTY 2 cut(s) 427, 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XmaJI CCTAGG 1 cut(s) 557
XspI CTAG 4 cut(s) 35, 270, 317, 558
Zsp2I ATGCAT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.