RchiOBHm_Chr5g0019941

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001185
Physical Location & Seq
Forward (+)
14333329 .. 14334579
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30006

Sequence Viewer

Length: 822 bp
ATGGGGACAGGGTGGTCACAAGATGAGGCCTCACAGTCACAGCAGCTGAGTCCTGCAGAAGCAAGTAACAAGGCAGTAGTGGAGAAAGTAACAGAAGCAGCGCCTAGTGGCTCTAAACCCATGGCAAAAGTGGAGAAATCAGCTAGTTTTCAGGCCAAATCTTCAGCCGATCACAACCAGGAGGCCAAAGTAGTGAAAGAAGTCAAGGGAACAGAGAAACCGACAGTTACACAAGTGAAGCAGCAGCAGCAAACGCTGCAGCAGCTCCCACATAATTACGAAGCTATTGTGAAAGATGCCGACACTCCAATCAACAAGTCACCGGAACATCTGTTTAAGTATTGGGTTGACAAGAAGTCCAACAACTGCTTCATGGTGTATCCAAGGGATCTCACAATCACTTGGGCTGAAGACAATCGTTACTGGCACTGGCCTTCCCTGGAAGAAACAAGTAATGTCTTCATCGATGCAGCTGAACTGTTAAATGTATGCTGGCTTGAAGTTCATGGAAAAATTGATTCCACAAAGCTGTCACCAGGAACTCTATATGAAGTAGTATTTATAGTGATGTTGAAAGCTGCAGCTTATGGATGGAAAGTTCCTGTCAATGTCAGACTCAGTCTCCCAGATGGTAACAAGCAAGAACGTAAAGCTGATCTGAATAAAATCCCTAGAGAACAATGGACAGAGATACCAGTAGGCGAGTTTAGAGCATCAACAGGATTACTTGGGAACATTGAATTTTCGTTGTATGAATATGAAGATGGGAAATGGAAGAGTGGACTTCTGATCAAGGGCGTTGCCGTTCGGCCTAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

273

Amino Acids

30.71

Weight (kDa)

6.55

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 122 - 271 1.2e-44 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 2 cut(s) 147, 429
AgsI TTSAA 3 cut(s) 500, 574, 740
AhdI GACNNNNNGTC 1 cut(s) 355
AjnI CCWGG 3 cut(s) 177, 438, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
AluBI AGCT 9 cut(s) 46, 143, 265, 284, 473, 529, 578, 584, 653
AluI AGCT 9 cut(s) 46, 143, 265, 284, 473, 529, 578, 584, 653
Alw26I GTCTC 1 cut(s) 626
AlwI GGATC 1 cut(s) 396
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 5 cut(s) 27, 153, 183, 431, 809
ApoI RAATTY 1 cut(s) 740
AspLEI GCGC 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 312, 525
BbsI GAAGAC 2 cut(s) 417, 451
BccI CCATC 3 cut(s) 585, 623, 758
BceAI ACGGC 1 cut(s) 788
BciT130I CCWGG 3 cut(s) 179, 440, 537
BciVI GTATCC 1 cut(s) 390
BclI TGATCA 1 cut(s) 789
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 3 cut(s) 105, 144, 672
BfmI CTRYAG 3 cut(s) 54, 257, 579
BfoI RGCGCY 1 cut(s) 104
BfuI GTATCC 1 cut(s) 390
Bme1390I CCNGG 3 cut(s) 179, 440, 537
BmeRI GACNNNNNGTC 1 cut(s) 355
BmrFI CCNGG 3 cut(s) 179, 440, 537
BmsI GCATC 3 cut(s) 286, 457, 722
BpiI GAAGAC 2 cut(s) 417, 451
Bsa29I ATCGAT 1 cut(s) 465
BsaJI CCNNGG 3 cut(s) 120, 383, 438
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bse1I ACTGG 3 cut(s) 428, 434, 695
BseBI CCWGG 3 cut(s) 179, 440, 537
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 120, 383, 438
BseGI GGATG 1 cut(s) 596
BseMII CTCAG 2 cut(s) 38, 631
BseNI ACTGG 3 cut(s) 428, 434, 695
BshFI GGCC 5 cut(s) 29, 155, 185, 433, 811
BshVI ATCGAT 1 cut(s) 465
BsiSI CCGG 1 cut(s) 323
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 626
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 5 cut(s) 29, 155, 185, 433, 811
Bsp143I GATC 4 cut(s) 169, 388, 655, 789
Bsp19I CCATGG 1 cut(s) 120
BspANI GGCC 5 cut(s) 29, 155, 185, 433, 811
BspCNI CTCAG 2 cut(s) 39, 630
BspDI ATCGAT 1 cut(s) 465
BspMAI CTGCAG 3 cut(s) 58, 261, 583
BspPI GGATC 1 cut(s) 396
BsrI ACTGG 3 cut(s) 428, 434, 695
BssECI CCNNGG 3 cut(s) 120, 383, 438
BssMI GATC 4 cut(s) 169, 388, 655, 789
BssT1I CCWWGG 2 cut(s) 120, 383
Bst2UI CCWGG 3 cut(s) 179, 440, 537
Bst4CI ACNGT 3 cut(s) 36, 226, 480
Bst6I CTCTTC 1 cut(s) 770
BstAPI GCANNNNNTGC 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 2 cut(s) 47, 617
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 596
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 4 cut(s) 172, 391, 658, 792
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 4 cut(s) 169, 388, 655, 789
BstMWI GCNNNNNNNGC 4 cut(s) 247, 253, 256, 262
BstNI CCWGG 3 cut(s) 179, 440, 537
BstSCI CCNGG 3 cut(s) 177, 438, 535
BstSFI CTRYAG 3 cut(s) 54, 257, 579
BstV2I GAAGAC 2 cut(s) 417, 451
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
Bsu15I ATCGAT 1 cut(s) 465
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 5 cut(s) 29, 155, 185, 433, 811
BsuTUI ATCGAT 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 596
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 494
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 1 cut(s) 103
ClaI ATCGAT 1 cut(s) 465
CviAII CATG 3 cut(s) 121, 373, 506
DdeI CTNAG 2 cut(s) 47, 617
DpnI GATC 4 cut(s) 171, 390, 657, 791
DpnII GATC 4 cut(s) 169, 388, 655, 789
DrdI GACNNNNNNGTC 1 cut(s) 13
DriI GACNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 770
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 770
Eco130I CCWWGG 2 cut(s) 120, 383
Eco147I AGGCCT 1 cut(s) 29
Eco57I CTGAAG 2 cut(s) 147, 429
EcoRII CCWGG 3 cut(s) 177, 438, 535
EcoT14I CCWWGG 2 cut(s) 120, 383
ErhI CCWWGG 2 cut(s) 120, 383
FaeI CATG 3 cut(s) 124, 376, 509
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 120, 372, 505
FbaI TGATCA 1 cut(s) 789
FokI GGATG 1 cut(s) 603
FspBI CTAG 3 cut(s) 105, 144, 672
GlaI GCGC 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 5 cut(s) 29, 155, 185, 433, 811
HapII CCGG 1 cut(s) 323
HhaI GCGC 1 cut(s) 103
Hin1II CATG 3 cut(s) 124, 376, 509
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 3 cut(s) 49, 518, 615
HpaII CCGG 1 cut(s) 323
HphI GGTGA 2 cut(s) 312, 525
Hpy166II GTNNAC 2 cut(s) 349, 782
Hpy188I TCNGA 3 cut(s) 614, 660, 789
Hpy8I GTNNAC 2 cut(s) 349, 782
HpyAV CCTTC 1 cut(s) 444
HpyCH4III ACNGT 3 cut(s) 36, 226, 480
HpyCH4IV ACGT 1 cut(s) 646
HpyCH4V TGCA 4 cut(s) 56, 259, 470, 581
HpyF10VI GCNNNNNNNGC 4 cut(s) 247, 253, 256, 262
HpyF3I CTNAG 2 cut(s) 47, 617
HpySE526I ACGT 1 cut(s) 646
Hsp92II CATG 3 cut(s) 124, 376, 509
HspAI GCGC 1 cut(s) 101
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 4 cut(s) 169, 388, 655, 789
LmnI GCTCC 1 cut(s) 270
LweI GCATC 3 cut(s) 286, 457, 722
MaeI CTAG 3 cut(s) 105, 144, 672
MaeII ACGT 1 cut(s) 646
MaeIII GTNAC 9 cut(s) 15, 36, 65, 88, 226, 318, 419, 531, 632
MalI GATC 4 cut(s) 171, 390, 657, 791
MboI GATC 4 cut(s) 169, 388, 655, 789
MboII GAAGA 6 cut(s) 153, 422, 451, 455, 773, 787
MflI RGATCY 1 cut(s) 388
MluCI AATT 3 cut(s) 274, 513, 740
MlyI GAGTC 2 cut(s) 58, 609
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 3 cut(s) 19, 40, 175
MseI TTAA 2 cut(s) 336, 482
MspA1I CMGCKG 2 cut(s) 46, 473
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 3 cut(s) 179, 440, 537
MvaI CCWGG 3 cut(s) 179, 440, 537
MwoI GCNNNNNNNGC 4 cut(s) 247, 253, 256, 262
NcoI CCATGG 1 cut(s) 120
NdeII GATC 4 cut(s) 169, 388, 655, 789
NlaIII CATG 3 cut(s) 124, 376, 509
NmuCI GTSAC 4 cut(s) 15, 36, 318, 531
PceI AGGCCT 1 cut(s) 29
PfeI GAWTC 1 cut(s) 518
PflFI GACNNNGTC 1 cut(s) 618
PleI GAGTC 2 cut(s) 57, 609
PpsI GAGTC 2 cut(s) 57, 609
Psp6I CCWGG 3 cut(s) 177, 438, 535
PspGI CCWGG 3 cut(s) 177, 438, 535
PstI CTGCAG 3 cut(s) 58, 261, 583
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 388
PsyI GACNNNGTC 1 cut(s) 618
PvuII CAGCTG 2 cut(s) 46, 473
SaqAI TTAA 2 cut(s) 336, 482
Sau3AI GATC 4 cut(s) 169, 388, 655, 789
SchI GAGTC 2 cut(s) 58, 609
ScrFI CCNGG 3 cut(s) 179, 440, 537
SfaNI GCATC 3 cut(s) 286, 457, 722
SfcI CTRYAG 3 cut(s) 54, 257, 579
Sse9I AATT 3 cut(s) 274, 513, 740
SseBI AGGCCT 1 cut(s) 29
SspMI CTAG 3 cut(s) 105, 144, 672
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 177, 438, 535
StyI CCWWGG 2 cut(s) 120, 383
TaaI ACNGT 3 cut(s) 36, 226, 480
TaiI ACGT 1 cut(s) 649
TaqI TCGA 1 cut(s) 465
TasI AATT 3 cut(s) 274, 513, 740
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 2 cut(s) 336, 482
Tru9I TTAA 2 cut(s) 336, 482
TscAI CASTG 1 cut(s) 434
TseFI GTSAC 4 cut(s) 15, 36, 318, 531
Tsp45I GTSAC 4 cut(s) 15, 36, 318, 531
TspDTI ATGAA 6 cut(s) 361, 451, 494, 564, 768, 774
TspRI CASTG 1 cut(s) 434
Tth111I GACNNNGTC 1 cut(s) 618
XapI RAATTY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XspI CTAG 3 cut(s) 105, 144, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.