pycom05g32190

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001185
Physical Location & Seq
Reverse (-)
31706877 .. 31708305
1429 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32190.4

Sequence Viewer

Length: 852 bp
ATGCTTGATATCTACATTTTAAAGAATAAAAGATTTGCAACGCAATGCATCTTTCTTCTGCATTATCTGAGAATGAATGCTATTCTTTTGCATAATGTTCGCAACGCAAGGCATCTTTCTTATTCTCATCCGCATGTTGCGGGGACTTGTGGAAATGACTGGCTTAGCCCTGTCTTCCTACGTATATATAGTGAAAACATGATTGAAGAACTGCACGAAGAGATGGAACCGGCAGCAAATACGTCGTTCCCACATAAGTATCAAGACATTCTAGGAGATTTTAAAGCACGTATCGACACGTCCTCACCTGAAAATGTCCGTTTGGCGCTCGGAGGAACAGGAATTTTCGACCAACAGAGAAAGCATAGGTATTGGGTTGATGAAAAGGGCAGGAACTGTTTTATGGTGTATGCAAGAGCTCTCAGAATCGCTTGGAGTGGAAACAGACGTCAGTGGCGCTGGTACACCGAGAGAGAAAACAACAGCGATGTCCTCGTTGAGGTTGCTGAACTGTTACGTGCATATTGGCTAGAAGTGCACGGGGTTTTTGAGACCGGGTACCTGACACCCGGTACTAAGTATGAAGTTTCATATGTGATCAAGATGAAGGAACCAAGCTCCGGATGGGATGATAAGGTCGACTTCAGCCTCACTCTCCCTCAGAACCCGACGAGGGGCCTGAATATCAATAAAACAGATCTGAAGCAGATATCAAGGGACGATTGGATTTCTATACGTGTTGACGAATTTACATCTGGGCAATCTGGCAAGATGGATTTTATGCTCCGTCAACCTGGGAGACTGAAGACAGGGCTTGTTATCAAGGGTGTCACCATTCGTCCTACAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

284

Amino Acids

32.98

Weight (kDa)

9.01

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 451
Acc65I GGTACC 1 cut(s) 558
AccB1I GGYRCC 1 cut(s) 558
AccI GTMKAC 1 cut(s) 639
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 2 cut(s) 131, 140
AcsI RAATTY 2 cut(s) 342, 746
AcuI CTGAAG 3 cut(s) 628, 722, 824
AcyI GRCGYC 1 cut(s) 448
AfaI GTAC 3 cut(s) 464, 560, 574
AfiI CCNNNNNNNGG 4 cut(s) 499, 620, 673, 674
AflIII ACRYGT 2 cut(s) 297, 736
AgsI TTSAA 1 cut(s) 206
AjiI CACGTC 1 cut(s) 300
AjnI CCWGG 1 cut(s) 793
AluBI AGCT 2 cut(s) 419, 618
AluI AGCT 2 cut(s) 419, 618
Alw21I GWGCWC 2 cut(s) 421, 540
Alw26I GTCTC 2 cut(s) 545, 793
Alw44I GTGCAC 1 cut(s) 536
AlwNI CAGNNNCTG 1 cut(s) 396
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 1 cut(s) 676
ApaLI GTGCAC 1 cut(s) 536
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 2 cut(s) 342, 746
Asp718I GGTACC 1 cut(s) 558
AspLEI GCGC 2 cut(s) 328, 459
AspS9I GGNCC 1 cut(s) 676
AsuC2I CCSGG 2 cut(s) 556, 570
AsuHPI GGTGA 2 cut(s) 297, 823
BaeGI GKGCMC 1 cut(s) 540
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BanI GGYRCC 1 cut(s) 558
BanII GRGCYC 1 cut(s) 421
BbsI GAAGAC 2 cut(s) 166, 812
Bbv12I GWGCWC 2 cut(s) 421, 540
BbvI GCAGC 1 cut(s) 245
BccI CCATC 3 cut(s) 217, 618, 766
BcgI CGANNNNNNTGC 4 cut(s) 80, 114, 225, 259
BciT130I CCWGG 1 cut(s) 795
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 2 cut(s) 556, 570
BcoDI GTCTC 2 cut(s) 545, 793
BfaI CTAG 2 cut(s) 272, 530
BfoI RGCGCY 2 cut(s) 329, 460
BglII AGATCT 1 cut(s) 697
BisI GCNGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 3 cut(s) 556, 570, 795
BmgBI CACGTC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 4 cut(s) 228, 560, 612, 677
BmrFI CCNGG 3 cut(s) 556, 570, 795
BmsI GCATC 2 cut(s) 57, 121
BpiI GAAGAC 2 cut(s) 166, 812
Bpu1102I GCTNAGC 1 cut(s) 164
BpuMI CCSGG 2 cut(s) 556, 570
BsaAI YACGTR 4 cut(s) 182, 290, 518, 737
BsaHI GRCGYC 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 1 cut(s) 794
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 4 cut(s) 499, 620, 673, 674
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 50
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 1 cut(s) 795
BseDI CCNNGG 1 cut(s) 794
BseGI GGATG 3 cut(s) 127, 629, 634
BseLI CCNNNNNNNGG 4 cut(s) 499, 620, 673, 674
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 3 cut(s) 59, 436, 674
BseNI ACTGG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 1 cut(s) 678
BshNI GGYRCC 1 cut(s) 558
BsiHKAI GWGCWC 2 cut(s) 421, 540
BsiSI CCGG 4 cut(s) 230, 555, 570, 621
BslFI GGGAC 2 cut(s) 157, 731
BslI CCNNNNNNNGG 4 cut(s) 499, 620, 673, 674
BsmAI GTCTC 2 cut(s) 545, 793
BsmFI GGGAC 2 cut(s) 157, 731
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 1 cut(s) 678
Bso31I GGTCTC 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 421, 540
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 2 cut(s) 597, 697
Bsp1720I GCTNAGC 1 cut(s) 164
BspACI CCGC 2 cut(s) 131, 140
BspANI GGCC 1 cut(s) 678
BspCNI CTCAG 3 cut(s) 60, 435, 673
BspEI TCCGGA 1 cut(s) 620
BspLI GGNNCC 4 cut(s) 228, 560, 612, 677
BspT107I GGYRCC 1 cut(s) 558
BspTNI GGTCTC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 1 cut(s) 164
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 794
BssMI GATC 2 cut(s) 597, 697
BssNI GRCGYC 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 2 cut(s) 398, 513
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 1 cut(s) 448
BstBAI YACGTR 4 cut(s) 182, 290, 518, 737
BstDEI CTNAG 5 cut(s) 68, 164, 422, 576, 660
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 3 cut(s) 127, 629, 634
BstH2I RGCGCY 2 cut(s) 329, 460
BstHHI GCGC 2 cut(s) 328, 459
BstKTI GATC 2 cut(s) 600, 700
BstMAI GTCTC 2 cut(s) 545, 793
BstMBI GATC 2 cut(s) 597, 697
BstMWI GCNNNNNNNGC 1 cut(s) 535
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 1 cut(s) 137
BstSCI CCNGG 3 cut(s) 554, 568, 793
BstSLI GKGCMC 1 cut(s) 540
BstSNI TACGTA 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 245
BstV2I GAAGAC 2 cut(s) 166, 812
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BsuRI GGCC 1 cut(s) 678
BtgZI GCGATG 1 cut(s) 501
BtrI CACGTC 1 cut(s) 300
BtsCI GGATG 3 cut(s) 127, 629, 634
BtsIMutI CAGTG 1 cut(s) 458
CaiI CAGNNNCTG 1 cut(s) 396
CfoI GCGC 2 cut(s) 328, 459
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 676
Csp6I GTAC 3 cut(s) 463, 559, 573
CviAII CATG 2 cut(s) 134, 199
CviJI RGCY 8 cut(s) 163, 168, 419, 529, 618, 648, 678, 814
CviKI_1 RGCY 8 cut(s) 163, 168, 419, 529, 618, 648, 678, 814
CviQI GTAC 3 cut(s) 463, 559, 573
DdeI CTNAG 5 cut(s) 68, 164, 422, 576, 660
DpnI GATC 2 cut(s) 599, 699
DpnII GATC 2 cut(s) 597, 697
DraI TTTAAA 2 cut(s) 21, 283
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 419
Eco105I TACGTA 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 421
Eco31I GGTCTC 1 cut(s) 545
Eco32I GATATC 2 cut(s) 10, 711
Eco53kI GAGCTC 1 cut(s) 419
Eco57I CTGAAG 3 cut(s) 628, 722, 824
EcoICRI GAGCTC 1 cut(s) 419
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 1 cut(s) 793
EcoRV GATATC 2 cut(s) 10, 711
EcoT22I ATGCAT 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 421
FaeI CATG 2 cut(s) 137, 202
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 2 cut(s) 157, 731
FatI CATG 2 cut(s) 133, 198
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 592
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 3 cut(s) 114, 636, 641
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 2 cut(s) 272, 530
GlaI GCGC 2 cut(s) 327, 458
GluI GCNGC 1 cut(s) 234
HaeII RGCGCY 2 cut(s) 329, 460
HaeIII GGCC 1 cut(s) 678
HapII CCGG 4 cut(s) 230, 555, 570, 621
HhaI GCGC 2 cut(s) 328, 459
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 2 cut(s) 137, 202
Hin6I GCGC 2 cut(s) 326, 457
HinP1I GCGC 2 cut(s) 326, 457
HincII GTYRAC 3 cut(s) 640, 742, 791
HindII GTYRAC 3 cut(s) 640, 742, 791
HinfI GANTC 1 cut(s) 426
HpaII CCGG 4 cut(s) 230, 555, 570, 621
HphI GGTGA 2 cut(s) 297, 823
Hpy166II GTNNAC 5 cut(s) 465, 538, 640, 742, 791
Hpy188I TCNGA 5 cut(s) 69, 332, 425, 663, 702
Hpy188III TCNNGA 3 cut(s) 263, 601, 621
Hpy8I GTNNAC 5 cut(s) 465, 538, 640, 742, 791
Hpy99I CGWCG 2 cut(s) 247, 673
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 2 cut(s) 398, 513
HpyCH4IV ACGT 7 cut(s) 181, 242, 289, 299, 448, 517, 736
HpyCH4V TGCA 8 cut(s) 38, 48, 61, 91, 214, 413, 521, 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 535
HpyF3I CTNAG 5 cut(s) 68, 164, 422, 576, 660
HpySE526I ACGT 7 cut(s) 181, 242, 289, 299, 448, 517, 736
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 2 cut(s) 137, 202
HspAI GCGC 2 cut(s) 326, 457
Kpn2I TCCGGA 1 cut(s) 620
KpnI GGTACC 1 cut(s) 562
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 2 cut(s) 597, 697
LmnI GCTCC 2 cut(s) 623, 789
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 2 cut(s) 57, 121
MaeI CTAG 2 cut(s) 272, 530
MaeII ACGT 7 cut(s) 181, 242, 289, 299, 448, 517, 736
MaeIII GTNAC 2 cut(s) 513, 829
MalI GATC 2 cut(s) 599, 699
MboI GATC 2 cut(s) 597, 697
MboII GAAGA 5 cut(s) 47, 166, 218, 230, 817
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 2 cut(s) 421, 540
MluCI AATT 2 cut(s) 342, 746
MnlI CCTC 7 cut(s) 313, 326, 493, 503, 659, 666, 669
Mph1103I ATGCAT 1 cut(s) 50
MroI TCCGGA 1 cut(s) 620
MseI TTAA 2 cut(s) 20, 282
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 4 cut(s) 230, 555, 570, 621
MspR9I CCNGG 3 cut(s) 556, 570, 795
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 1 cut(s) 795
MwoI GCNNNNNNNGC 1 cut(s) 535
NciI CCSGG 2 cut(s) 556, 570
NdeI CATATG 1 cut(s) 592
NdeII GATC 2 cut(s) 597, 697
NlaIII CATG 2 cut(s) 137, 202
NlaIV GGNNCC 4 cut(s) 228, 560, 612, 677
NmuCI GTSAC 1 cut(s) 829
NsiI ATGCAT 1 cut(s) 50
NspI RCATGY 1 cut(s) 137
PctI GAATGC 1 cut(s) 82
PfeI GAWTC 1 cut(s) 426
PkrI GCNGC 1 cut(s) 235
Ppu21I YACGTR 4 cut(s) 182, 290, 518, 737
Psp124BI GAGCTC 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 793
PspGI CCWGG 1 cut(s) 793
PspN4I GGNNCC 4 cut(s) 228, 560, 612, 677
PspPI GGNCC 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 396
PsuI RGATCY 1 cut(s) 697
RsaI GTAC 3 cut(s) 464, 560, 574
RsaNI GTAC 3 cut(s) 463, 559, 573
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 421
SalI GTCGAC 1 cut(s) 638
SaqAI TTAA 2 cut(s) 20, 282
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 2 cut(s) 597, 697
Sau96I GGNCC 1 cut(s) 676
ScrFI CCNGG 3 cut(s) 556, 570, 795
SduI GDGCHC 2 cut(s) 421, 540
SfaNI GCATC 2 cut(s) 57, 121
SmiMI CAYNNNNRTG 1 cut(s) 132
SnaBI TACGTA 1 cut(s) 182
Sse9I AATT 2 cut(s) 342, 746
SsiI CCGC 2 cut(s) 131, 140
SspMI CTAG 2 cut(s) 272, 530
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 3 cut(s) 554, 568, 793
TaaI ACNGT 2 cut(s) 398, 513
TaiI ACGT 7 cut(s) 184, 245, 292, 302, 451, 520, 739
TaqI TCGA 3 cut(s) 294, 348, 639
TasI AATT 2 cut(s) 342, 746
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 2 cut(s) 20, 282
Tru9I TTAA 2 cut(s) 20, 282
TscAI CASTG 1 cut(s) 458
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 5 cut(s) 89, 396, 579, 597, 620
TspGWI ACGGA 2 cut(s) 308, 776
TspRI CASTG 1 cut(s) 458
VneI GTGCAC 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 2 cut(s) 342, 746
XceI RCATGY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmiI GTMKAC 1 cut(s) 639
XspI CTAG 2 cut(s) 272, 530
ZraI GACGTC 1 cut(s) 449
Zsp2I ATGCAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.