RLG00000024434

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001185
Physical Location & Seq
Reverse (-)
35805385 .. 35806292
908 bp
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UTR
Exon/CDS
Intron
RLM00000024434

Sequence Viewer

Length: 558 bp
ATGGGAACGGGGTGCTCACGATATGAGGCCTCACAGTCGCAGCAGCTGAGACCTGGAGAACCAAGTAACAAGAAGTATTGGGTTGACAAGAAGTCCAACAACTGCTTCATGGTGTATCCAAGGGATTTCTGGATCGTTTGGGCTGAGGACAAACGTTACTGGCACTGGTCTTCCCTGGAAGAAACAAGTAATGTCTTTATTGATGTCCCTGAACTGTTAAAGGTCTGCTGGCTTGAAGTTCATGGAAATATTGATTCCACAAAGCTGTCACCAGGAACCCTATATGAAGTAGTCTTTATAGTGATGTTGAAAGCTGCAGCTTATGGATGGAAAGTTCCTGTCAATGTCCACCTCACTCTCCCAGATGGTAACAAGCAAGAACGTAAAGCTGATCTGAATAAAATCCCTAGAGAACAATGGACAGAGATACCAGTAGGCGAGTTTAGAGCATCAACAGGATTACTTGGGAACATTGGATTTTCGATGTATGAATATGGCGATGAGAAATGGAAGAGTGGACTTCTGATCAAGGGCGTTGCCGTTCGGCCTAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

186

Amino Acids

21.31

Weight (kDa)

8.34

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 34 - 183 8.6e-42 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 154
AclWI GGATC 1 cut(s) 140
AgsI TTSAA 2 cut(s) 236, 310
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 52, 174, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
AluBI AGCT 5 cut(s) 46, 265, 314, 320, 389
AluI AGCT 5 cut(s) 46, 265, 314, 320, 389
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 1 cut(s) 140
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 2 cut(s) 27, 545
ApeKI GCWGC 4 cut(s) 40, 43, 314, 317
AsuHPI GGTGA 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 4 cut(s) 52, 55, 301, 329
BccI CCATC 2 cut(s) 321, 359
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 3 cut(s) 54, 176, 273
BciVI GTATCC 1 cut(s) 126
BclI TGATCA 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 315
BfuI GTATCC 1 cut(s) 126
BisI GCNGC 4 cut(s) 41, 44, 315, 318
BlsI GCNGC 4 cut(s) 42, 45, 316, 319
Bme1390I CCNGG 3 cut(s) 54, 176, 273
BmeRI GACNNNNNGTC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 3 cut(s) 54, 176, 273
BmsI GCATC 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 75
Bpu10I CCTNAGC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 2 cut(s) 119, 174
Bse1I ACTGG 3 cut(s) 164, 170, 431
BseBI CCWGG 3 cut(s) 54, 176, 273
BseDI CCNNGG 2 cut(s) 119, 174
BseGI GGATG 1 cut(s) 332
BseMII CTCAG 2 cut(s) 38, 135
BseNI ACTGG 3 cut(s) 164, 170, 431
BseXI GCAGC 4 cut(s) 52, 55, 301, 329
BshFI GGCC 2 cut(s) 29, 547
BsiHKAI GWGCWC 1 cut(s) 17
BslFI GGGAC 1 cut(s) 191
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 2 cut(s) 29, 547
Bso31I GGTCTC 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 3 cut(s) 132, 391, 525
BspANI GGCC 2 cut(s) 29, 547
BspCNI CTCAG 2 cut(s) 39, 136
BspLI GGNNCC 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 43
BsrI ACTGG 3 cut(s) 164, 170, 431
BssECI CCNNGG 2 cut(s) 119, 174
BssMI GATC 3 cut(s) 132, 391, 525
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 3 cut(s) 54, 176, 273
Bst4CI ACNGT 2 cut(s) 36, 216
Bst6I CTCTTC 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 2 cut(s) 47, 144
BstF5I GGATG 1 cut(s) 332
BstKTI GATC 3 cut(s) 135, 394, 528
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 3 cut(s) 132, 391, 525
BstNI CCWGG 3 cut(s) 54, 176, 273
BstSCI CCNGG 3 cut(s) 52, 174, 271
BstSFI CTRYAG 1 cut(s) 315
BstV1I GCAGC 4 cut(s) 52, 55, 301, 329
BstV2I GAAGAC 1 cut(s) 162
BsuI GTATCC 1 cut(s) 126
BsuRI GGCC 2 cut(s) 29, 547
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 163
Cac8I GCNNGC 1 cut(s) 230
CaiI CAGNNNCTG 1 cut(s) 46
CviAII CATG 2 cut(s) 109, 242
CviJI RGCY 9 cut(s) 29, 46, 143, 232, 265, 314, 320, 389, 547
CviKI_1 RGCY 9 cut(s) 29, 46, 143, 232, 265, 314, 320, 389, 547
DdeI CTNAG 2 cut(s) 47, 144
DpnI GATC 3 cut(s) 134, 393, 527
DpnII GATC 3 cut(s) 132, 391, 525
DriI GACNNNNNGTC 1 cut(s) 91
Eam1104I CTCTTC 1 cut(s) 506
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 119
Eco147I AGGCCT 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 43
EcoRII CCWGG 3 cut(s) 52, 174, 271
EcoT14I CCWWGG 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 2 cut(s) 112, 245
FaiI YATR 9 cut(s) 24, 110, 243, 283, 285, 299, 324, 489, 495
FaqI GGGAC 1 cut(s) 191
FatI CATG 2 cut(s) 108, 241
FbaI TGATCA 1 cut(s) 525
Fnu4HI GCNGC 4 cut(s) 41, 44, 315, 318
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 41, 44, 315, 318
FspBI CTAG 1 cut(s) 408
GluI GCNGC 4 cut(s) 41, 44, 315, 318
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 2 cut(s) 29, 547
Hin1II CATG 2 cut(s) 112, 245
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HinfI GANTC 1 cut(s) 254
HphI GGTGA 1 cut(s) 261
Hpy166II GTNNAC 3 cut(s) 85, 349, 518
Hpy188I TCNGA 2 cut(s) 396, 525
Hpy188III TCNNGA 2 cut(s) 18, 130
Hpy8I GTNNAC 3 cut(s) 85, 349, 518
HpyCH4III ACNGT 2 cut(s) 36, 216
HpyCH4IV ACGT 2 cut(s) 154, 382
HpyCH4V TGCA 1 cut(s) 317
HpyF3I CTNAG 2 cut(s) 47, 144
HpySE526I ACGT 2 cut(s) 154, 382
Hsp92II CATG 2 cut(s) 112, 245
Ksp22I TGATCA 1 cut(s) 525
Kzo9I GATC 3 cut(s) 132, 391, 525
Lsp1109I GCAGC 4 cut(s) 52, 55, 301, 329
LweI GCATC 1 cut(s) 458
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 154, 382
MaeIII GTNAC 4 cut(s) 65, 155, 267, 368
MalI GATC 3 cut(s) 134, 393, 527
MboI GATC 3 cut(s) 132, 391, 525
MboII GAAGA 3 cut(s) 162, 191, 523
MhlI GDGCHC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 4 cut(s) 19, 40, 139, 362
MseI TTAA 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 3 cut(s) 54, 176, 273
MvaI CCWGG 3 cut(s) 54, 176, 273
NdeII GATC 3 cut(s) 132, 391, 525
NlaIII CATG 2 cut(s) 112, 245
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 267
PceI AGGCCT 1 cut(s) 29
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 4 cut(s) 42, 45, 316, 319
Psp1406I AACGTT 1 cut(s) 154
Psp6I CCWGG 3 cut(s) 52, 174, 271
PspGI CCWGG 3 cut(s) 52, 174, 271
PspN4I GGNNCC 1 cut(s) 277
PstI CTGCAG 1 cut(s) 319
PstNI CAGNNNCTG 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 46
SaqAI TTAA 1 cut(s) 218
SatI GCNGC 4 cut(s) 41, 44, 315, 318
Sau3AI GATC 3 cut(s) 132, 391, 525
ScrFI CCNGG 3 cut(s) 54, 176, 273
SduI GDGCHC 1 cut(s) 17
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 1 cut(s) 315
SseBI AGGCCT 1 cut(s) 29
SspI AATATT 1 cut(s) 250
SspMI CTAG 1 cut(s) 408
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 52, 174, 271
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 2 cut(s) 36, 216
TaiI ACGT 2 cut(s) 157, 385
TaqI TCGA 1 cut(s) 482
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 218
Tru9I TTAA 1 cut(s) 218
TscAI CASTG 1 cut(s) 170
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 4 cut(s) 40, 43, 314, 317
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 4 cut(s) 97, 230, 300, 504
TspRI CASTG 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.