Rw5G012880

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001185
Physical Location & Seq
Forward (+)
15276247 .. 15277164
918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012880.1

Sequence Viewer

Length: 702 bp
ATGGGGACAGGGTGGTCACAAGATGAAGCCTCACAGTCGCAGCAGCTGAGGCCTGGAGAACCAAGTAACAAGCTCCTAATCGATTTTGTGCGACTGTTAAACAAGCGCACCTCAGAAAAGAAATTTGAAATCAACCAACAAAAACATGAACGACATCACTATCAATCATTATTCTTTTTTCGAGTATTAGCACTATTACTGTTATTATTACAGAAGTACTGGGTTGACAAGAAGTCCAACAACTGCTTCATGGTGTATCCAAGGGATCTCACAATCACTTGGGCTGACGACAAACGTTACTGGCACTGGCCTTCCCTGGCAGAAACAAGTAATGTCTTCATCGATGTTGCTGAACTGTTAAGGGTATGCTGGCTTGAAGTTCATGGAAAAATTGATTCCACAAAGCTGTCACCAGGAACCCTATATGAAGTAGTATTTATAGTGATGTTGAAAGCTGCAGCTTATGGATGGAAAGTTCCTGTCAATGTCAGCCTCACTCTCCCAGATGGTAACAAGCAAGAGCGTAAAGCTGATCTGAATAAAATCCCAAGAGAACAATGGACAGAGATACCAGTAGGCGAGTTTAGAGCATCACCAGGATTACTTGGGAACATTGAAATTTCGATGTATCAATATGATGATGGCGGGATATGGAAGAGCGGACTTTTGATCAAGGGCGTTGCCGTTCGGCCTAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

233

Amino Acids

26.95

Weight (kDa)

9.08

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 81 - 231 1.7e-43 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AccBSI CCGCTC 1 cut(s) 660
AciI CCGC 2 cut(s) 645, 660
AclI AACGTT 1 cut(s) 295
AclWI GGATC 1 cut(s) 273
AcsI RAATTY 2 cut(s) 122, 618
AfaI GTAC 1 cut(s) 218
AgsI TTSAA 4 cut(s) 128, 377, 451, 617
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 4 cut(s) 52, 315, 412, 595
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AloI GAACNNNNNNTCC 2 cut(s) 459, 491
AluBI AGCT 7 cut(s) 46, 73, 406, 455, 461, 530, 699
AluI AGCT 7 cut(s) 46, 73, 406, 455, 461, 530, 699
AlwI GGATC 1 cut(s) 273
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 3 cut(s) 50, 308, 689
ApeKI GCWGC 4 cut(s) 40, 43, 455, 458
ApoI RAATTY 2 cut(s) 122, 618
AspLEI GCGC 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 402, 585
BbsI GAAGAC 1 cut(s) 328
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 4 cut(s) 52, 55, 442, 470
BccI CCATC 3 cut(s) 462, 500, 635
BceAI ACGGC 1 cut(s) 668
BciT130I CCWGG 4 cut(s) 54, 317, 414, 597
BciVI GTATCC 1 cut(s) 267
BclI TGATCA 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 456
BfuI GTATCC 1 cut(s) 267
BisI GCNGC 4 cut(s) 41, 44, 456, 459
BlsI GCNGC 4 cut(s) 42, 45, 457, 460
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 4 cut(s) 54, 317, 414, 597
BmeRI GACNNNNNGTC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 4 cut(s) 54, 317, 414, 597
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 1 cut(s) 599
BmuI ACTGGG 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 328
BpmI CTGGAG 1 cut(s) 75
Bpu10I CCTNAGC 1 cut(s) 47
Bsa29I ATCGAT 2 cut(s) 81, 342
BsaJI CCNNGG 2 cut(s) 260, 315
Bse1I ACTGG 4 cut(s) 224, 305, 311, 572
BseBI CCWGG 4 cut(s) 54, 317, 414, 597
BseCI ATCGAT 2 cut(s) 81, 342
BseDI CCNNGG 2 cut(s) 260, 315
BseGI GGATG 1 cut(s) 473
BseMII CTCAG 2 cut(s) 38, 126
BseNI ACTGG 4 cut(s) 224, 305, 311, 572
BseXI GCAGC 4 cut(s) 52, 55, 442, 470
BshFI GGCC 3 cut(s) 52, 310, 691
BshVI ATCGAT 2 cut(s) 81, 342
BslFI GGGAC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 3 cut(s) 52, 310, 691
Bsp143I GATC 3 cut(s) 265, 532, 669
BspACI CCGC 2 cut(s) 645, 660
BspANI GGCC 3 cut(s) 52, 310, 691
BspCNI CTCAG 2 cut(s) 39, 125
BspDI ATCGAT 2 cut(s) 81, 342
BspLI GGNNCC 1 cut(s) 418
BspMAI CTGCAG 1 cut(s) 460
BspPI GGATC 1 cut(s) 273
BspQI GCTCTTC 1 cut(s) 650
BsrBI CCGCTC 1 cut(s) 660
BsrI ACTGG 4 cut(s) 224, 305, 311, 572
BssECI CCNNGG 2 cut(s) 260, 315
BssMI GATC 3 cut(s) 265, 532, 669
BssT1I CCWWGG 1 cut(s) 260
Bst2UI CCWGG 4 cut(s) 54, 317, 414, 597
Bst4CI ACNGT 4 cut(s) 36, 96, 201, 357
Bst6I CTCTTC 1 cut(s) 650
BstC8I GCNNGC 1 cut(s) 371
BstDEI CTNAG 2 cut(s) 47, 112
BstF5I GGATG 1 cut(s) 473
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 3 cut(s) 268, 535, 672
BstMBI GATC 3 cut(s) 265, 532, 669
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 4 cut(s) 54, 317, 414, 597
BstSCI CCNGG 4 cut(s) 52, 315, 412, 595
BstSFI CTRYAG 1 cut(s) 456
BstV1I GCAGC 4 cut(s) 52, 55, 442, 470
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
Bsu15I ATCGAT 2 cut(s) 81, 342
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 3 cut(s) 52, 310, 691
BsuTUI ATCGAT 2 cut(s) 81, 342
BtsCI GGATG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 371
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 1 cut(s) 108
ClaI ATCGAT 2 cut(s) 81, 342
Csp6I GTAC 1 cut(s) 217
CviAII CATG 3 cut(s) 146, 250, 383
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 2 cut(s) 47, 112
DpnI GATC 3 cut(s) 267, 534, 671
DpnII GATC 3 cut(s) 265, 532, 669
DrdI GACNNNNNNGTC 1 cut(s) 13
DriI GACNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 650
Eam1105I GACNNNNNGTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 650
Eco130I CCWWGG 1 cut(s) 260
Eco147I AGGCCT 1 cut(s) 52
EcoRII CCWGG 4 cut(s) 52, 315, 412, 595
EcoT14I CCWWGG 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 3 cut(s) 149, 253, 386
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 145, 249, 382
FauI CCCGC 1 cut(s) 638
FbaI TGATCA 1 cut(s) 669
Fnu4HI GCNGC 4 cut(s) 41, 44, 456, 459
FokI GGATG 1 cut(s) 480
Fsp4HI GCNGC 4 cut(s) 41, 44, 456, 459
GlaI GCGC 1 cut(s) 107
GluI GCNGC 4 cut(s) 41, 44, 456, 459
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 3 cut(s) 52, 310, 691
HhaI GCGC 1 cut(s) 108
Hin1II CATG 3 cut(s) 149, 253, 386
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 395
HphI GGTGA 2 cut(s) 402, 585
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 115, 537
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 321
HpyCH4III ACNGT 4 cut(s) 36, 96, 201, 357
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 1 cut(s) 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
HpyF3I CTNAG 2 cut(s) 47, 112
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 3 cut(s) 149, 253, 386
HspAI GCGC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 3 cut(s) 265, 532, 669
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 1 cut(s) 78
Lsp1109I GCAGC 4 cut(s) 52, 55, 442, 470
LweI GCATC 1 cut(s) 599
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 5 cut(s) 15, 65, 296, 408, 509
MalI GATC 3 cut(s) 267, 534, 671
MbiI CCGCTC 1 cut(s) 660
MboI GATC 3 cut(s) 265, 532, 669
MboII GAAGA 2 cut(s) 328, 667
MflI RGATCY 1 cut(s) 265
MluCI AATT 3 cut(s) 122, 390, 618
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 4 cut(s) 40, 42, 121, 503
MseI TTAA 2 cut(s) 98, 359
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 4 cut(s) 54, 317, 414, 597
MvaI CCWGG 4 cut(s) 54, 317, 414, 597
MwoI GCNNNNNNNGC 1 cut(s) 49
NdeII GATC 3 cut(s) 265, 532, 669
NlaIII CATG 3 cut(s) 149, 253, 386
NlaIV GGNNCC 1 cut(s) 418
NmuCI GTSAC 2 cut(s) 15, 408
PceI AGGCCT 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 650
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 4 cut(s) 42, 45, 457, 460
Psp1406I AACGTT 1 cut(s) 295
Psp6I CCWGG 4 cut(s) 52, 315, 412, 595
PspGI CCWGG 4 cut(s) 52, 315, 412, 595
PspN4I GGNNCC 1 cut(s) 418
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 46
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 2 cut(s) 98, 359
SatI GCNGC 4 cut(s) 41, 44, 456, 459
Sau3AI GATC 3 cut(s) 265, 532, 669
ScaI AGTACT 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 54, 317, 414, 597
SetI ASST 9 cut(s) 48, 75, 113, 298, 408, 457, 463, 532, 701
SfaNI GCATC 1 cut(s) 599
SfcI CTRYAG 1 cut(s) 456
Sse9I AATT 3 cut(s) 122, 390, 618
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 2 cut(s) 645, 660
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 4 cut(s) 52, 315, 412, 595
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 4 cut(s) 36, 96, 201, 357
TaiI ACGT 1 cut(s) 298
TaqI TCGA 4 cut(s) 81, 181, 342, 623
TasI AATT 3 cut(s) 122, 390, 618
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 98, 359
Tru9I TTAA 2 cut(s) 98, 359
TscAI CASTG 1 cut(s) 311
TseFI GTSAC 2 cut(s) 15, 408
TseI GCWGC 4 cut(s) 40, 43, 455, 458
Tsp45I GTSAC 2 cut(s) 15, 408
TspDTI ATGAA 6 cut(s) 39, 162, 238, 328, 371, 441
TspRI CASTG 1 cut(s) 311
XapI RAATTY 2 cut(s) 122, 618
XcmI CCANNNNNNNNNTGG 1 cut(s) 555
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.