pycom05g23210

Protein PHLOEM PROTEIN 2-LIKE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001185
Physical Location & Seq
Reverse (-)
25160884 .. 25162289
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g23210.1

Sequence Viewer

Length: 897 bp
ATGGGGACTGGGTGGTCACAAGATGAGGCCTCACAGTCACGGTCGCAGCCGCCTAGTGAGCAACCGACTAATAATAAGGTAGCGGCAGAGAAAGAAACAGAAGCAAAGCCGACTGTCTCTAAACCCACAGTGGTAGAGAGAACAGCTGCTGATCATCAGGCCAAGCCCTCGGCGAATAACAACCAGGTAGCCGCAGCAGCGAAAGAAGTCAAGGAAATAGAGAAAAGGACAGCAGCACAGCAAGTTAGGCAGCAGCAGTTCCCACATAACTATGAAGCCCTTGTGAAAGATGCTGACACACCAATAAACAAGTCCTCAATTGAAAACCTCTTTCAGCAGCTCCAAGCAGGAGTTGTCTTAAACCAAAAGAAAAAGAAGTATTGGGTTGATAAAAAGTCCAACAACTGTTTTATGGTGTATGCAAGAGATCTCTCAATCACTTGGGCCGAAGACAATCGTTACTGGCACTGGTACTCCCTGCAAGAAACCAGTGACGTCTTCATTGATGCTGCTGAACTGTTGAATGTATGTTGGCTAGAAGTGCACGGGAAGTTTGAAACCGCAAACCTGTCACCCGGAACTCTTTATGAAGTTGCATTTGTGGTCAAGCTGAATGCTGCAGATTATGGATGGGAAGTTCCAGTCAATTTCAGGCTCACTCTCCCTGATGGTACCAGGCAGTGCCGTAAAGTTAATCTGATGCAAACCCCAAGAGGACAATGGGTCGAGATCCCAGTTGGCGAGTTTAAAGCATCGCCTGAGAAATCTGGCGACATGGAGTTTTCGATGTATGAATATGACAGCGGGAACTGGAAGAAAGGGCTTGTTATCAAGGGTGTCGTCATTCGTCCTACAAACCAAGTTGCACAGTACTCAGCAACTTCGCTTACGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009605 GO:0009607 GO:0009625 GO:0030246 GO:0043207 GO:0043394 GO:0050896 GO:0051704 GO:0051707 GO:0097367
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

299

Amino Acids

33.63

Weight (kDa)

6.47

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 135 - 284 1.8e-47 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
AatII GACGTC 1 cut(s) 498
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 1 cut(s) 671
AciI CCGC 5 cut(s) 50, 83, 192, 561, 804
AclWI GGATC 1 cut(s) 724
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 3 cut(s) 473, 673, 872
AgsI TTSAA 3 cut(s) 323, 523, 557
AhdI GACNNNNNGTC 1 cut(s) 722
AjnI CCWGG 2 cut(s) 183, 674
AloI GAACNNNNNNTCC 2 cut(s) 621, 653
AluBI AGCT 4 cut(s) 146, 340, 610, 894
AluI AGCT 4 cut(s) 146, 340, 610, 894
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 1 cut(s) 121
Alw44I GTGCAC 1 cut(s) 542
AlwI GGATC 1 cut(s) 724
AlwNI CAGNNNCTG 1 cut(s) 149
AoxI GGCC 3 cut(s) 27, 159, 444
ApaLI GTGCAC 1 cut(s) 542
Asp718I GGTACC 1 cut(s) 671
AspS9I GGNCC 1 cut(s) 444
AsuC2I CCSGG 1 cut(s) 576
AsuHPI GGTGA 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 546
BanI GGYRCC 1 cut(s) 671
BbsI GAAGAC 2 cut(s) 456, 490
Bbv12I GWGCWC 1 cut(s) 546
BccI CCATC 2 cut(s) 624, 662
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 2 cut(s) 185, 676
BclI TGATCA 1 cut(s) 151
BcnI CCSGG 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 2 cut(s) 54, 536
BfmI CTRYAG 1 cut(s) 618
BglII AGATCT 1 cut(s) 427
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 3 cut(s) 185, 576, 676
BmeRI GACNNNNNGTC 1 cut(s) 722
BmgT120I GGNCC 1 cut(s) 444
BmiI GGNNCC 1 cut(s) 673
BmrFI CCNGG 3 cut(s) 185, 576, 676
BmrI ACTGGG 2 cut(s) 18, 728
BmsI GCATC 4 cut(s) 280, 496, 690, 761
BmuI ACTGGG 2 cut(s) 18, 728
BpiI GAAGAC 2 cut(s) 456, 490
BpuMI CCSGG 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bse1I ACTGG 7 cut(s) 13, 467, 473, 489, 641, 734, 815
BseBI CCWGG 2 cut(s) 185, 676
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 635
BseMII CTCAG 2 cut(s) 750, 888
BseNI ACTGG 7 cut(s) 13, 467, 473, 489, 641, 734, 815
BseSI GKGCMC 1 cut(s) 546
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 3 cut(s) 29, 161, 446
BshNI GGYRCC 1 cut(s) 671
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 546
BsiSI CCGG 1 cut(s) 576
BslFI GGGAC 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 3 cut(s) 29, 161, 446
Bsp1286I GDGCHC 1 cut(s) 546
Bsp143I GATC 3 cut(s) 151, 427, 729
BspACI CCGC 5 cut(s) 50, 83, 192, 561, 804
BspANI GGCC 3 cut(s) 29, 161, 446
BspCNI CTCAG 2 cut(s) 751, 887
BspLI GGNNCC 1 cut(s) 673
BspMAI CTGCAG 1 cut(s) 622
BspPI GGATC 1 cut(s) 724
BspT107I GGYRCC 1 cut(s) 671
BsrI ACTGG 7 cut(s) 13, 467, 473, 489, 641, 734, 815
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 3 cut(s) 151, 427, 729
BssNI GRCGYC 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 185, 676
Bst4CI ACNGT 7 cut(s) 36, 42, 115, 130, 407, 519, 870
BstACI GRCGYC 1 cut(s) 495
BstDEI CTNAG 2 cut(s) 759, 874
BstF5I GGATG 1 cut(s) 635
BstKTI GATC 3 cut(s) 154, 430, 732
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 3 cut(s) 151, 427, 729
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 4 cut(s) 58, 197, 247, 541
BstNI CCWGG 2 cut(s) 185, 676
BstSCI CCNGG 3 cut(s) 183, 574, 674
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 1 cut(s) 546
BstV2I GAAGAC 2 cut(s) 456, 490
BstX2I RGATCY 2 cut(s) 427, 729
BstYI RGATCY 2 cut(s) 427, 729
BsuRI GGCC 3 cut(s) 29, 161, 446
BtgZI GCGATG 1 cut(s) 738
BtsCI GGATG 1 cut(s) 635
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 4 cut(s) 135, 466, 496, 686
CaiI CAGNNNCTG 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 444
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 3 cut(s) 472, 672, 871
CviAII CATG 1 cut(s) 775
CviQI GTAC 3 cut(s) 472, 672, 871
DdeI CTNAG 2 cut(s) 759, 874
DpnI GATC 3 cut(s) 153, 429, 731
DpnII GATC 3 cut(s) 151, 427, 729
DraI TTTAAA 1 cut(s) 748
DrdI GACNNNNNNGTC 1 cut(s) 13
DriI GACNNNNNGTC 1 cut(s) 722
DseDI GACNNNNNNGTC 1 cut(s) 13
Eam1105I GACNNNNNGTC 1 cut(s) 722
Eco147I AGGCCT 1 cut(s) 29
EcoRII CCWGG 2 cut(s) 183, 674
FaeI CATG 1 cut(s) 778
FaqI GGGAC 1 cut(s) 19
FatI CATG 1 cut(s) 774
FauI CCCGC 1 cut(s) 797
FbaI TGATCA 1 cut(s) 151
FokI GGATG 1 cut(s) 642
FspBI CTAG 2 cut(s) 54, 536
HaeIII GGCC 3 cut(s) 29, 161, 446
HapII CCGG 1 cut(s) 576
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 1 cut(s) 778
HpaII CCGG 1 cut(s) 576
HphI GGTGA 1 cut(s) 564
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 1 cut(s) 699
Hpy188III TCNNGA 1 cut(s) 727
Hpy8I GTNNAC 1 cut(s) 544
HpyCH4III ACNGT 7 cut(s) 36, 42, 115, 130, 407, 519, 870
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 7 cut(s) 422, 481, 544, 596, 620, 703, 866
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 197, 247, 541
HpyF3I CTNAG 2 cut(s) 759, 874
HpySE526I ACGT 1 cut(s) 495
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 1 cut(s) 778
KpnI GGTACC 1 cut(s) 675
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 3 cut(s) 151, 427, 729
LmnI GCTCC 1 cut(s) 345
LweI GCATC 4 cut(s) 280, 496, 690, 761
MabI ACCWGGT 1 cut(s) 183
MaeI CTAG 2 cut(s) 54, 536
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 5 cut(s) 15, 36, 458, 491, 570
MalI GATC 3 cut(s) 153, 429, 731
MboI GATC 3 cut(s) 151, 427, 729
MboII GAAGA 3 cut(s) 461, 490, 826
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 2 cut(s) 427, 729
MhlI GDGCHC 1 cut(s) 546
MluCI AATT 2 cut(s) 318, 646
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 6 cut(s) 19, 40, 178, 325, 338, 707
MseI TTAA 3 cut(s) 359, 693, 747
MslI CAYNNNNRTG 1 cut(s) 270
MspA1I CMGCKG 2 cut(s) 146, 804
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 3 cut(s) 185, 576, 676
MunI CAATTG 1 cut(s) 318
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 2 cut(s) 185, 676
MwoI GCNNNNNNNGC 4 cut(s) 58, 197, 247, 541
NciI CCSGG 1 cut(s) 576
NdeII GATC 3 cut(s) 151, 427, 729
NlaIII CATG 1 cut(s) 778
NlaIV GGNNCC 1 cut(s) 673
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 4 cut(s) 15, 36, 491, 570
PceI AGGCCT 1 cut(s) 29
PctI GAATGC 1 cut(s) 619
Psp6I CCWGG 2 cut(s) 183, 674
PspGI CCWGG 2 cut(s) 183, 674
PspN4I GGNNCC 1 cut(s) 673
PspPI GGNCC 1 cut(s) 444
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 2 cut(s) 427, 729
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 473, 673, 872
RsaNI GTAC 3 cut(s) 472, 672, 871
RseI CAYNNNNRTG 1 cut(s) 270
SaqAI TTAA 3 cut(s) 359, 693, 747
Sau3AI GATC 3 cut(s) 151, 427, 729
Sau96I GGNCC 1 cut(s) 444
ScaI AGTACT 1 cut(s) 872
ScrFI CCNGG 3 cut(s) 185, 576, 676
SduI GDGCHC 1 cut(s) 546
SetI ASST 9 cut(s) 81, 148, 189, 330, 342, 498, 570, 612, 896
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 4 cut(s) 280, 496, 690, 761
SfcI CTRYAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 270
Sse9I AATT 2 cut(s) 318, 646
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 5 cut(s) 50, 83, 192, 561, 804
SspMI CTAG 2 cut(s) 54, 536
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 183, 574, 674
TaaI ACNGT 7 cut(s) 36, 42, 115, 130, 407, 519, 870
TaiI ACGT 1 cut(s) 498
TaqI TCGA 2 cut(s) 726, 785
TasI AATT 2 cut(s) 318, 646
TatI WGTACW 1 cut(s) 870
TauI GCSGC 3 cut(s) 52, 86, 194
Tru1I TTAA 3 cut(s) 359, 693, 747
Tru9I TTAA 3 cut(s) 359, 693, 747
TscAI CASTG 4 cut(s) 135, 473, 496, 686
TseFI GTSAC 4 cut(s) 15, 36, 491, 570
Tsp45I GTSAC 4 cut(s) 15, 36, 491, 570
TspDTI ATGAA 4 cut(s) 288, 490, 603, 807
TspRI CASTG 4 cut(s) 135, 473, 496, 686
VneI GTGCAC 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XspI CTAG 2 cut(s) 54, 536
ZraI GACGTC 1 cut(s) 496
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.