Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001300

Physical Location & Seq

Genomic Coordinates Reverse (-)

23422962 .. 23427903

Gene Structure 4942 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g30201.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2577 bp

ATGTCCGGACGCGGCCGAGGCCGGCATTACTCCGACGATCGCTCCAACCCCTCGCACGGCGGAGGCGGCAGAGGAGGTTCCATCAGCGGAGGAAGTTCAGGCGCCGTTGCTCCGGCGCTGACTCGAACTCAGACTCCTGCTGCTTATTCTCCTCCCCCGGCCTCGTCAAGTTCGGCACCGGTGGCTTCTTCGCTGAGTAGCGAGATGGAGCAGAAGTTGACATTGGAGACGACCACGGCGCAGGTGGCTGCTGTTCCGCCGGCGTCGTCGAAGGCGGTGTGGTTTCAGGAGAGGCCGGACTTAGTGACGGTCGGGAGGAAAATTCAGGTCAGAGCCAATCACTTCCTGGTGCAGGTTGCTGACAGAGACCTCCACCATTACGATGTTACCATAACCCCTGAAGTTACGTCGAAAAAGATACACAGAGAAGTGATAAACCAACTGGTTCATCTGTATTCCGAGACGCATTTAGGCAAGAGAATTCCGGCTTATGATGGCATGAAGAGCCTCTATACCGCAGGGCCATTGCCATTTTCTTCCAGAGAGTTTGTTGTCAAGTTACTTCCAAATGATGCAGGGTCATCAACTTCCAAAAGGAAAAGAAAGGATCGAGAGTTTAAGGTGGCTCTCTCTTTGGCCAATAAGCCAGACCTTCATCGACTACAACAGTTCTTGCGCTGCCAGCATGTGGAAACGCCTCAAGACGCGATACAAGTTCTTGATGTTGCTCTTAGAGCTACACCATCTGAAAAGTACACTGTTGTTGGGAGGTCATTTTTCTCCACTGAGCATGGACCGAAAGGCAGTCTAGGTGAAGGTTTGGAATTCTGGAGAGGGTTTTACCAAAGTCTACGGCCTACACAGCTAGGACTTTCCCTTAATATTGATGTGTCAGCAAGATCTTTTTTTGAACCCATTTTGGTCACCAAGTTTCTTGCTAAACATTTTAACTACAGTGATCGTTCCAAGCCTTTGCCTGATCGTGATAGTATGAAGACACTCTTCACTATGCGTAAAAATGTGTTTATAACACGTAATAATTCAATTTTGTATCTTATGTTTACTTGTGAAGATGAGAACAGATCAGTTGTTCAATATTATCGTAAGAAGTACAAGATTGATCTCAAATATGTAGCTTGGCCTGCCATTCAAGCTGGCAATGATTCAAAACCTACTTACTTACCTATGGAGATGATCAGGAAGAAATCCAACAATAGAGACGATAGAGAATCTCTTATAAGGAGGGAATTTGGATTACAAGTGAGAGAGGAAATGGCATTGGTCAAAGCACGAGTTTTACCATCTCCATTGAAAATGTTCAACGGTGGATTGCAATTTTGGGCATTTGTTAATTTCTCCAGACTATCCCAAGATATTACTTTCCCATTTCTTGAAGCTTTGGTCAATGTGTGTAACAGTAAGGGGATGGAATTTTTCTCCCAACCCTTAATTCCTGTACAGTCATCTCATCCTGGACAAATTGAGAAGGTACCCCGGGATATTCACAAGATGTCTGTTCAAAAGGGAAGACAACTTCAGTTATTAATTGTCATTTTGCCTGATTTTAGTGATTCATCTTATGGGAAGATTAAACGAACCTGTGAAACAGAATTAGGCATTGTGTCTCAGTGTTGTGAACCCAAAAAAGTAAAAACATTCAATACACAATATTTGGAAAATCTTGCCCTTAAGATTAATGTGAAGGTTGGTGGACGGAACACTGTTTTGAATGATGCAATTTGTAAGAAGCTGCCTGTTGTCACCGATGGCGTCCCCACAATCATCTTTGGAGCAGATGTTACTCATCCAGCAGCCGGGGATGATAGTAGTCCATCAGTGGCAGCAGTTGTGGGCTCAACAGATTGGCCAGACATCACAAACTACAGAGCAATTGTTTCTGCACAGCCTCACCGTGATGAAATTATTAAAGATCTTTATAAATCACACAAATCTGAGAAGGATTTGGTTCCTGGAGGAATGATAAGGGAACTTCTCGTTGCATTCTACAAACAAAACAGATTAAAGCCCTCAAGAATCATATTCTACAGAGATGGCGTTAGCGAAGGCCAATTCAGCCAAGTTTTGCTATATGAGATGGATGCTATAAGAAGGGCCTGTGCCTCTTTGGAGGAACGATATATGCCTACAGTAACGTTTGTGGTGGTACAGAAAAGGCATCATACTCGCCTTTTCCCTGCTGATCATAATAATTGTGGTCAGATGGACAGGAGTGGAAATATTCAACCAGGTACTGTTGTTGACACTGAGATTTGCCACCCTACTGAATTTGACTTTTACCTCAATAGTCATGCCGGAATCCAGGGAACAAGCCGTCCAGCACACTACCATGTGTTGTATGATGAAAACAGTTTCAGTGCAGATAGTTTGCAAGTTCTAACCAACAATTTATGTTATACTGCCTCCCGTGTACTACGCCCATTTGGTAGCTTTTCGAGCACGGCATTACATGACAGTGACTCATCGGATGGAGGTTCTATAGCTGGTGCGGGTGCACATGTTCTCCGAGCCCTTCCGGAGATCAAAGGAAATGTGAAAGAAGTGATGTTTTATTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009814 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016441 GO:0016458 GO:0019222 GO:0035821 GO:0040029 GO:0043207 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048519 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0098542 GO:0098586

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

859

Amino Acids

95.82

Weight (kDa)

9.26

Isoelectric Point (pI)

46.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	106 - 244	2.3e-36	N-terminal domain of argonaute
ArgoL1	PF08699	255 - 303	1.2e-18	Argonaute linker 1 domain
PAZ	PF02170	351 - 406	7.1e-10	PAZ domain
ArgoL2	PF16488	408 - 436	8.7e-06	Argonaute linker 2 domain
Piwi	PF02171	515 - 813	2e-96	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27880
fragaria_vesca	FvH4_3g30201 FvH4_3g30202
malus_domestica	MD03G1130600.v1.1 MD03G1254500.v1.1 MD11G1152200.v1.1 MD11G1152400.v1.1
prunus_persica	Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1
pyrus_communis	pycom01g05040 pycom11g12470
rosa_chinensis	RchiOBHm_Chr1g0362621 RchiOBHm_Chr5g0055991 RchiOBHm_Chr5g0056001 RchiOBHm_Chr5g0056021 RchiOBHm_Chr5g0056031 RchiOBHm_Chr5g0056051 RchiOBHm_Chr5g0056071 RchiOBHm_Chr5g0056081 RchiOBHm_Chr5g0056091
rosa_laevigata	RLG00000027633 RLG00000035041 RLG00000035044 RLG00000035045 RLG00000035046
rosa_roxburghii	Rroxscaffold_1G00024210 Rroxscaffold_1G00024220
rosa_rugosa	Rorug05G0297000 Rorug05G0297100 Rorug05G0297200 Rorug05G0297300 Rorug05G0297300 Rorug05G0297400
rosa_samantha	Rh1CG289700 Rh1DG302900 Rh5AG368000 Rh5AG368200 Rh5AG368300 Rh5CG401800 Rh5CG402100 Rh5CG402200 Rh5DG391500 Rh5DG391600 Rh5DG391700
rosa_wichuraiana	Rw0G004500 Rw1G027430 Rw5G034540 Rw5G034570 Rw5G034580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1028, 1238, 1938
AarI	CACCTGC	1 cut(s)	232
Acc36I	ACCTGC	2 cut(s)	232, 343
Acc65I	GGTACC	1 cut(s)	1489
AccB1I	GGYRCC	3 cut(s)	101, 175, 1489
AccB7I	CCANNNNNTGG	1 cut(s)	688
AccI	GTMKAC	1 cut(s)	850
AccII	CGCG	2 cut(s)	12, 707
AccIII	TCCGGA	2 cut(s)	5, 2533
AciI	CCGC	8 cut(s)	12, 60, 66, 87, 257, 275, 516, 2507
AclI	AACGTT	1 cut(s)	2153
AclWI	GGATC	1 cut(s)	615
AcoI	YGGCCR	3 cut(s)	13, 636, 1865
AcsI	RAATTY	6 cut(s)	321, 480, 824, 1247, 1430, 2285
AcuI	CTGAAG	2 cut(s)	420, 1520
AcyI	GRCGYC	3 cut(s)	102, 263, 1770
AfaI	GTAC	7 cut(s)	755, 1112, 1458, 1491, 2166, 2251, 2430
AfiI	CCNNNNNNNGG	4 cut(s)	56, 352, 688, 1814
AflII	CTTAAG	1 cut(s)	1688
AflIII	ACRYGT	2 cut(s)	1031, 2515
AgeI	ACCGGT	1 cut(s)	178
AjnI	CCWGG	5 cut(s)	345, 1471, 1969, 2245, 2319
AjuI	GAANNNNNNNTTGG	4 cut(s)	206, 238, 1434, 1466
AloI	GAACNNNNNNTCC	4 cut(s)	118, 150, 2317, 2349
AluBI	AGCT	8 cut(s)	737, 865, 1136, 1154, 1397, 1750, 2448, 2501
AluI	AGCT	8 cut(s)	737, 865, 1136, 1154, 1397, 1750, 2448, 2501
Alw21I	GWGCWC	2 cut(s)	2459, 2515
Alw26I	GTCTC	5 cut(s)	221, 360, 455, 1212, 1629
Alw44I	GTGCAC	1 cut(s)	2511
AlwI	GGATC	1 cut(s)	615
AlwNI	CAGNNNCTG	1 cut(s)	2252
Ama87I	CYCGRG	1 cut(s)	1494
Aor13HI	TCCGGA	2 cut(s)	5, 2533
ApaLI	GTGCAC	1 cut(s)	2511
ApeKI	GCWGC	6 cut(s)	140, 248, 678, 1750, 1811, 1841
ApoI	RAATTY	6 cut(s)	321, 480, 824, 1247, 1430, 2285
AseI	ATTAAT	2 cut(s)	1544, 1695
AsiGI	ACCGGT	1 cut(s)	178
Asp700I	GAANNNNTTC	1 cut(s)	1316
Asp718I	GGTACC	1 cut(s)	1489
AspLEI	GCGC	4 cut(s)	104, 118, 241, 678
AspS9I	GGNCC	3 cut(s)	521, 794, 2112
AsuC2I	CCSGG	4 cut(s)	158, 1495, 1496, 1816
AsuHPI	GGTGA	4 cut(s)	824, 916, 1753, 1901
AvaI	CYCGRG	1 cut(s)	1494
AvaII	GGWCC	1 cut(s)	794
BaeGI	GKGCMC	1 cut(s)	2515
BaeI	ACNNNNGTAYC	2 cut(s)	2241, 2274
BalI	TGGCCA	2 cut(s)	638, 1867
BanI	GGYRCC	3 cut(s)	101, 175, 1489
BanII	GRGCYC	2 cut(s)	1856, 2530
BauI	CACGAG	1 cut(s)	1290
BbsI	GAAGAC	2 cut(s)	1001, 1534
Bbv12I	GWGCWC	2 cut(s)	2459, 2515
BbvI	GCAGC	6 cut(s)	127, 235, 665, 1737, 1823, 1853
BceAI	ACGGC	6 cut(s)	73, 89, 252, 869, 2316, 2475
BcgI	CGANNNNNNTGC	2 cut(s)	2165, 2199
BciT130I	CCWGG	5 cut(s)	347, 1473, 1971, 2247, 2321
BclI	TGATCA	2 cut(s)	1194, 2200
BcnI	CCSGG	4 cut(s)	158, 1495, 1496, 1816
BcoDI	GTCTC	5 cut(s)	221, 360, 455, 1212, 1629
BfaI	CTAG	2 cut(s)	809, 866
BfmI	CTRYAG	5 cut(s)	952, 1882, 2044, 2145, 2496
BfoI	RGCGCY	2 cut(s)	105, 119
BfrI	CTTAAG	1 cut(s)	1688
BfuAI	ACCTGC	2 cut(s)	232, 343
BglII	AGATCT	2 cut(s)	899, 1930
BisI	GCNGC	8 cut(s)	13, 67, 141, 249, 679, 1751, 1812, 1842
BlsI	GCNGC	8 cut(s)	14, 68, 142, 250, 680, 1752, 1813, 1843
Bme1390I	CCNGG	9 cut(s)	158, 347, 1473, 1495, 1496, 1816, 1971, 2247, 2321
Bme18I	GGWCC	1 cut(s)	794
BmeT110I	CYCGRG	1 cut(s)	1494
BmgT120I	GGNCC	3 cut(s)	521, 794, 2112
BmiI	GGNNCC	5 cut(s)	79, 103, 177, 1491, 1968
BmrFI	CCNGG	9 cut(s)	158, 347, 1473, 1495, 1496, 1816, 1971, 2247, 2321
BmsI	GCATC	4 cut(s)	562, 1723, 2089, 2185
BpiI	GAAGAC	2 cut(s)	1001, 1534
BpmI	CTGGAG	3 cut(s)	850, 1342, 1992
BpuEI	CTTGAG	2 cut(s)	684, 2014
BpuMI	CCSGG	4 cut(s)	158, 1495, 1496, 1816
BsaAI	YACGTR	1 cut(s)	1034
BsaHI	GRCGYC	3 cut(s)	102, 263, 1770
BsaI	GGTCTC	1 cut(s)	360
BsaJI	CCNNGG	7 cut(s)	16, 156, 234, 1493, 1494, 1815, 2320
BsaWI	WCCGGW	3 cut(s)	5, 178, 2533
Bsc4I	CCNNNNNNNGG	4 cut(s)	56, 352, 688, 1814
Bse118I	RCCGGY	3 cut(s)	21, 178, 259
Bse1I	ACTGG	1 cut(s)	447
Bse3DI	GCAATG	2 cut(s)	524, 1165
BseAI	TCCGGA	2 cut(s)	5, 2533
BseBI	CCWGG	5 cut(s)	347, 1473, 1971, 2247, 2321
BseDI	CCNNGG	7 cut(s)	16, 156, 234, 1493, 1494, 1815, 2320
BseGI	GGATG	6 cut(s)	1431, 1468, 1804, 1825, 2104, 2491
BseLI	CCNNNNNNNGG	4 cut(s)	56, 352, 688, 1814
BseMI	GCAATG	2 cut(s)	524, 1165
BseMII	CTCAG	6 cut(s)	143, 185, 777, 1640, 1944, 2256
BseNI	ACTGG	1 cut(s)	447
BseRI	GAGGAG	2 cut(s)	87, 141
BseSI	GKGCMC	1 cut(s)	2515
BseX3I	CGGCCG	1 cut(s)	13
BseXI	GCAGC	6 cut(s)	127, 235, 665, 1737, 1823, 1853
BsgI	GTGCAG	3 cut(s)	371, 1884, 2397
Bsh1236I	CGCG	2 cut(s)	12, 707
Bsh1285I	CGRYCG	3 cut(s)	16, 40, 312
BshNI	GGYRCC	3 cut(s)	101, 175, 1489
BshTI	ACCGGT	1 cut(s)	178
BsiEI	CGRYCG	3 cut(s)	16, 40, 312
BsiHKAI	GWGCWC	2 cut(s)	2459, 2515
BsiHKCI	CYCGRG	1 cut(s)	1494
BslFI	GGGAC	1 cut(s)	1757
BslI	CCNNNNNNNGG	4 cut(s)	56, 352, 688, 1814
BsmAI	GTCTC	5 cut(s)	221, 360, 455, 1212, 1629
BsmBI	CGTCTC	3 cut(s)	221, 455, 1212
BsmFI	GGGAC	1 cut(s)	1757
BsmI	GAATGC	1 cut(s)	2000
Bso31I	GGTCTC	1 cut(s)	360
BsoBI	CYCGRG	1 cut(s)	1494
Bsp1286I	GDGCHC	4 cut(s)	1856, 2459, 2515, 2530
Bsp13I	TCCGGA	2 cut(s)	5, 2533
Bsp1407I	TGTACA	1 cut(s)	1456
BspACI	CCGC	8 cut(s)	12, 60, 66, 87, 257, 275, 516, 2507
BspCNI	CTCAG	6 cut(s)	142, 186, 778, 1639, 1945, 2257
BspEI	TCCGGA	2 cut(s)	5, 2533
BspFNI	CGCG	2 cut(s)	12, 707
BspLI	GGNNCC	5 cut(s)	79, 103, 177, 1491, 1968
BspMI	ACCTGC	2 cut(s)	232, 343
BspPI	GGATC	1 cut(s)	615
BspQI	GCTCTTC	1 cut(s)	497
BspT107I	GGYRCC	3 cut(s)	101, 175, 1489
BspTI	CTTAAG	1 cut(s)	1688
BspTNI	GGTCTC	1 cut(s)	360
BsrDI	GCAATG	2 cut(s)	524, 1165
BsrFI	RCCGGY	3 cut(s)	21, 178, 259
BsrGI	TGTACA	1 cut(s)	1456
BsrI	ACTGG	1 cut(s)	447
BssAI	RCCGGY	3 cut(s)	21, 178, 259
BssECI	CCNNGG	7 cut(s)	16, 156, 234, 1493, 1494, 1815, 2320
BssNI	GRCGYC	3 cut(s)	102, 263, 1770
BssSI	CACGAG	1 cut(s)	1290
Bst2BI	CACGAG	1 cut(s)	1290
Bst2UI	CCWGG	5 cut(s)	347, 1473, 1971, 2247, 2321
Bst6I	CTCTTC	2 cut(s)	497, 1007
BstACI	GRCGYC	3 cut(s)	102, 263, 1770
BstAFI	CTTAAG	1 cut(s)	1688
BstAUI	TGTACA	1 cut(s)	1456
BstBAI	YACGTR	1 cut(s)	1034
BstC8I	GCNNGC	5 cut(s)	23, 261, 683, 1143, 1156
BstDEI	CTNAG	8 cut(s)	129, 194, 301, 731, 786, 1626, 1953, 2265
BstDSI	CCRYGG	1 cut(s)	234
BstEII	GGTNACC	1 cut(s)	922
BstENI	CCTNNNNNAGG	1 cut(s)	350
BstF5I	GGATG	6 cut(s)	1431, 1468, 1804, 1825, 2104, 2491
BstFNI	CGCG	2 cut(s)	12, 707
BstH2I	RGCGCY	2 cut(s)	105, 119
BstHHI	GCGC	4 cut(s)	104, 118, 241, 678
BstMAI	GTCTC	5 cut(s)	221, 360, 455, 1212, 1629
BstMCI	CGRYCG	3 cut(s)	16, 40, 312
BstNI	CCWGG	5 cut(s)	347, 1473, 1971, 2247, 2321
BstNSI	RCATGY	2 cut(s)	689, 2519
BstPI	GGTNACC	1 cut(s)	922
BstSCI	CCNGG	9 cut(s)	156, 345, 1471, 1493, 1494, 1814, 1969, 2245, 2319
BstSFI	CTRYAG	5 cut(s)	952, 1882, 2044, 2145, 2496
BstSLI	GKGCMC	1 cut(s)	2515
BstUI	CGCG	2 cut(s)	12, 707
BstV1I	GCAGC	6 cut(s)	127, 235, 665, 1737, 1823, 1853
BstV2I	GAAGAC	2 cut(s)	1001, 1534
BstX2I	RGATCY	2 cut(s)	899, 1930
BstYI	RGATCY	2 cut(s)	899, 1930
BstZI	CGGCCG	1 cut(s)	13
BtgI	CCRYGG	1 cut(s)	234
BtsCI	GGATG	6 cut(s)	1431, 1468, 1804, 1825, 2104, 2491
BtsIMutI	CAGTG	9 cut(s)	756, 783, 961, 1634, 1719, 1842, 2262, 2380, 2479
BveI	ACCTGC	2 cut(s)	232, 343
Cac8I	GCNNGC	5 cut(s)	23, 261, 683, 1143, 1156
CaiI	CAGNNNCTG	1 cut(s)	2252
CfoI	GCGC	4 cut(s)	104, 118, 241, 678
Cfr10I	RCCGGY	3 cut(s)	21, 178, 259
Cfr13I	GGNCC	3 cut(s)	521, 794, 2112
Cfr9I	CCCGGG	1 cut(s)	1494
CseI	GACGC	5 cut(s)	18, 252, 472, 713, 1759
CsiI	ACCWGGT	1 cut(s)	2245
Csp6I	GTAC	7 cut(s)	754, 1111, 1457, 1490, 2165, 2250, 2429
CspAI	ACCGGT	1 cut(s)	178
CviAII	CATG	7 cut(s)	499, 686, 791, 2309, 2348, 2468, 2516
CviQI	GTAC	7 cut(s)	754, 1111, 1457, 1490, 2165, 2250, 2429
DdeI	CTNAG	8 cut(s)	129, 194, 301, 731, 786, 1626, 1953, 2265
DinI	GGCGCC	1 cut(s)	103
EaeI	YGGCCR	3 cut(s)	13, 636, 1865
EagI	CGGCCG	1 cut(s)	13
Eam1104I	CTCTTC	2 cut(s)	497, 1007
EarI	CTCTTC	2 cut(s)	497, 1007
EciI	GGCGGA	2 cut(s)	75, 246
EclXI	CGGCCG	1 cut(s)	13
Eco24I	GRGCYC	2 cut(s)	1856, 2530
Eco31I	GGTCTC	1 cut(s)	360
Eco47I	GGWCC	1 cut(s)	794
Eco52I	CGGCCG	1 cut(s)	13
Eco57I	CTGAAG	2 cut(s)	420, 1520
Eco88I	CYCGRG	1 cut(s)	1494
Eco91I	GGTNACC	1 cut(s)	922
EcoNI	CCTNNNNNAGG	1 cut(s)	350
EcoO109I	RGGNCCY	1 cut(s)	2112
EcoO65I	GGTNACC	1 cut(s)	922
EcoRI	GAATTC	2 cut(s)	480, 824
EcoRII	CCWGG	5 cut(s)	345, 1471, 1969, 2245, 2319
EcoT38I	GRGCYC	2 cut(s)	1856, 2530
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
Esp3I	CGTCTC	3 cut(s)	221, 455, 1212
FaeI	CATG	7 cut(s)	502, 689, 794, 2312, 2351, 2471, 2519
FalI	AAGNNNNNCTT	2 cut(s)	986, 1018
FaqI	GGGAC	1 cut(s)	1757
FatI	CATG	7 cut(s)	498, 685, 790, 2308, 2347, 2467, 2515
FauI	CCCGC	1 cut(s)	2500
FbaI	TGATCA	2 cut(s)	1194, 2200
FblI	GTMKAC	1 cut(s)	850
Fnu4HI	GCNGC	8 cut(s)	13, 67, 141, 249, 679, 1751, 1812, 1842
FokI	GGATG	6 cut(s)	1438, 1455, 1791, 1832, 2111, 2498
FriOI	GRGCYC	2 cut(s)	1856, 2530
Fsp4HI	GCNGC	8 cut(s)	13, 67, 141, 249, 679, 1751, 1812, 1842
FspBI	CTAG	2 cut(s)	809, 866
GlaI	GCGC	4 cut(s)	103, 117, 240, 677
GluI	GCNGC	8 cut(s)	13, 67, 141, 249, 679, 1751, 1812, 1842
GsuI	CTGGAG	3 cut(s)	850, 1342, 1992
HaeII	RGCGCY	2 cut(s)	105, 119
HgaI	GACGC	5 cut(s)	18, 252, 472, 713, 1759
HhaI	GCGC	4 cut(s)	104, 118, 241, 678
Hin1I	GRCGYC	3 cut(s)	102, 263, 1770
Hin1II	CATG	7 cut(s)	502, 689, 794, 2312, 2351, 2471, 2519
Hin6I	GCGC	4 cut(s)	102, 116, 239, 676
HinP1I	GCGC	4 cut(s)	102, 116, 239, 676
HincII	GTYRAC	2 cut(s)	219, 2260
HindII	GTYRAC	2 cut(s)	219, 2260
HindIII	AAGCTT	1 cut(s)	1395
HinfI	GANTC	8 cut(s)	121, 133, 1163, 1229, 1571, 2034, 2316, 2477
HphI	GGTGA	4 cut(s)	824, 916, 1753, 1901
Hpy166II	GTNNAC	9 cut(s)	219, 756, 851, 1062, 1637, 1712, 2260, 2429, 2513
Hpy188I	TCNGA	9 cut(s)	34, 132, 332, 460, 748, 1954, 2220, 2485, 2525
Hpy8I	GTNNAC	9 cut(s)	219, 756, 851, 1062, 1637, 1712, 2260, 2429, 2513
Hpy99I	CGWCG	4 cut(s)	38, 268, 271, 412
HpyAV	CCTTC	9 cut(s)	265, 662, 809, 1480, 1696, 1951, 2057, 2103, 2540
HpyCH4IV	ACGT	3 cut(s)	407, 1033, 2153
HpyCH4V	TGCA	9 cut(s)	352, 575, 1333, 1736, 1901, 2000, 2378, 2389, 2513
HpyF3I	CTNAG	8 cut(s)	129, 194, 301, 731, 786, 1626, 1953, 2265
HpySE526I	ACGT	3 cut(s)	407, 1033, 2153
Hsp92I	GRCGYC	3 cut(s)	102, 263, 1770
Hsp92II	CATG	7 cut(s)	502, 689, 794, 2312, 2351, 2471, 2519
HspAI	GCGC	4 cut(s)	102, 116, 239, 676
KasI	GGCGCC	1 cut(s)	101
Kpn2I	TCCGGA	2 cut(s)	5, 2533
KpnI	GGTACC	1 cut(s)	1493
KroI	GCCGGC	2 cut(s)	21, 259
KroNI	GCCGGC	2 cut(s)	23, 261
Ksp22I	TGATCA	2 cut(s)	1194, 2200
LguI	GCTCTTC	1 cut(s)	497
LmnI	GCTCC	4 cut(s)	47, 115, 208, 1790
Lsp1109I	GCAGC	6 cut(s)	127, 235, 665, 1737, 1823, 1853
LweI	GCATC	4 cut(s)	562, 1723, 2089, 2185
MabI	ACCWGGT	1 cut(s)	2245
MaeI	CTAG	2 cut(s)	809, 866
MaeII	ACGT	3 cut(s)	407, 1033, 2153
MboII	GAAGA	9 cut(s)	180, 514, 528, 994, 1006, 1082, 1213, 1539, 1597
MfeI	CAATTG	1 cut(s)	1890
MflI	RGATCY	2 cut(s)	899, 1930
MhlI	GDGCHC	4 cut(s)	1856, 2459, 2515, 2530
MlsI	TGGCCA	2 cut(s)	638, 1867
MluNI	TGGCCA	2 cut(s)	638, 1867
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	3 cut(s)	115, 127, 2471
MmeI	TCCRAC	3 cut(s)	57, 69, 1233
Mox20I	TGGCCA	2 cut(s)	638, 1867
MreI	CGCCGGCG	1 cut(s)	259
MroI	TCCGGA	2 cut(s)	5, 2533
MroNI	GCCGGC	2 cut(s)	21, 259
MroXI	GAANNNNTTC	1 cut(s)	1316
MscI	TGGCCA	2 cut(s)	638, 1867
MslI	CAYNNNNRTG	4 cut(s)	381, 1914, 2346, 2472
Msp20I	TGGCCA	2 cut(s)	638, 1867
MspA1I	CMGCKG	1 cut(s)	87
MspCI	CTTAAG	1 cut(s)	1688
MspR9I	CCNGG	9 cut(s)	158, 347, 1473, 1495, 1496, 1816, 1971, 2247, 2321
MunI	CAATTG	1 cut(s)	1890
Mva1269I	GAATGC	1 cut(s)	2000
MvaI	CCWGG	5 cut(s)	347, 1473, 1971, 2247, 2321
MvnI	CGCG	2 cut(s)	12, 707
NaeI	GCCGGC	2 cut(s)	23, 261
NarI	GGCGCC	1 cut(s)	102
NciI	CCSGG	4 cut(s)	158, 1495, 1496, 1816
NgoMIV	GCCGGC	2 cut(s)	21, 259
NlaIII	CATG	7 cut(s)	502, 689, 794, 2312, 2351, 2471, 2519
NlaIV	GGNNCC	5 cut(s)	79, 103, 177, 1491, 1968
NmeAIII	GCCGAG	1 cut(s)	41
NmuCI	GTSAC	4 cut(s)	304, 922, 1759, 2474
NspI	RCATGY	2 cut(s)	689, 2519
PaqCI	CACCTGC	1 cut(s)	232
PciI	ACATGT	1 cut(s)	2515
PciSI	GCTCTTC	1 cut(s)	497
PctI	GAATGC	1 cut(s)	2000
PdiI	GCCGGC	2 cut(s)	23, 261
PdmI	GAANNNNTTC	1 cut(s)	1316
PfeI	GAWTC	5 cut(s)	1163, 1229, 1571, 2034, 2316
PflMI	CCANNNNNTGG	1 cut(s)	688
PfoI	TCCNGGA	2 cut(s)	1471, 1969
PinAI	ACCGGT	1 cut(s)	178
PkrI	GCNGC	8 cut(s)	14, 68, 142, 250, 680, 1752, 1813, 1843
Ple19I	CGATCG	1 cut(s)	40
PleI	GAGTC	3 cut(s)	115, 127, 2471
PluTI	GGCGCC	1 cut(s)	105
PpsI	GAGTC	3 cut(s)	115, 127, 2471
Ppu21I	YACGTR	1 cut(s)	1034
PscI	ACATGT	1 cut(s)	2515
PshBI	ATTAAT	2 cut(s)	1544, 1695
PsiI	TTATAA	3 cut(s)	1028, 1238, 1938
Psp1406I	AACGTT	1 cut(s)	2153
Psp6I	CCWGG	5 cut(s)	345, 1471, 1969, 2245, 2319
PspEI	GGTNACC	1 cut(s)	922
PspGI	CCWGG	5 cut(s)	345, 1471, 1969, 2245, 2319
PspN4I	GGNNCC	5 cut(s)	79, 103, 177, 1491, 1968
PspPI	GGNCC	3 cut(s)	521, 794, 2112
PstNI	CAGNNNCTG	1 cut(s)	2252
PsuI	RGATCY	2 cut(s)	899, 1930
PvuI	CGATCG	1 cut(s)	40
RsaI	GTAC	7 cut(s)	755, 1112, 1458, 1491, 2166, 2251, 2430
RsaNI	GTAC	7 cut(s)	754, 1111, 1457, 1490, 2165, 2250, 2429
RseI	CAYNNNNRTG	4 cut(s)	381, 1914, 2346, 2472
SapI	GCTCTTC	1 cut(s)	497
SatI	GCNGC	8 cut(s)	13, 67, 141, 249, 679, 1751, 1812, 1842
Sau96I	GGNCC	3 cut(s)	521, 794, 2112
SchI	GAGTC	3 cut(s)	115, 127, 2471
ScrFI	CCNGG	9 cut(s)	158, 347, 1473, 1495, 1496, 1816, 1971, 2247, 2321
SduI	GDGCHC	4 cut(s)	1856, 2459, 2515, 2530
SexAI	ACCWGGT	1 cut(s)	2245
SfaNI	GCATC	4 cut(s)	562, 1723, 2089, 2185
SfcI	CTRYAG	5 cut(s)	952, 1882, 2044, 2145, 2496
SfoI	GGCGCC	1 cut(s)	103
SgrAI	CRCCGGYG	2 cut(s)	178, 259
SinI	GGWCC	1 cut(s)	794
SmaI	CCCGGG	1 cut(s)	1496
SmiMI	CAYNNNNRTG	4 cut(s)	381, 1914, 2346, 2472
SmlI	CTYRAG	3 cut(s)	699, 1688, 2029
SmoI	CTYRAG	3 cut(s)	699, 1688, 2029
SsiI	CCGC	8 cut(s)	12, 60, 66, 87, 257, 275, 516, 2507
SspDI	GGCGCC	1 cut(s)	101
SspI	AATATT	4 cut(s)	883, 1097, 1670, 2239
SspMI	CTAG	2 cut(s)	809, 866
StyD4I	CCNGG	9 cut(s)	156, 345, 1471, 1493, 1494, 1814, 1969, 2245, 2319
TaiI	ACGT	3 cut(s)	410, 1036, 2156
TaqI	TCGA	6 cut(s)	124, 269, 410, 610, 658, 2453
TaqII	GACCGA	1 cut(s)	811
TatI	WGTACW	4 cut(s)	753, 1110, 1456, 2428
TauI	GCSGC	2 cut(s)	15, 69
TfiI	GAWTC	5 cut(s)	1163, 1229, 1571, 2034, 2316
TscAI	CASTG	9 cut(s)	763, 790, 961, 1634, 1726, 1842, 2269, 2380, 2479
TseFI	GTSAC	4 cut(s)	304, 922, 1759, 2474
TseI	GCWGC	6 cut(s)	140, 248, 678, 1750, 1811, 1841
Tsp45I	GTSAC	4 cut(s)	304, 922, 1759, 2474
TspDTI	ATGAA	7 cut(s)	437, 515, 644, 1007, 1563, 1932, 2376
TspGWI	ACGGA	1 cut(s)	1729
TspMI	CCCGGG	1 cut(s)	1494
TspRI	CASTG	9 cut(s)	763, 790, 961, 1634, 1726, 1842, 2269, 2380, 2479
Van91I	CCANNNNNTGG	1 cut(s)	688
Vha464I	CTTAAG	1 cut(s)	1688
VneI	GTGCAC	1 cut(s)	2511
VpaK11BI	GGWCC	1 cut(s)	794
VspI	ATTAAT	2 cut(s)	1544, 1695
XagI	CCTNNNNNAGG	1 cut(s)	350
XapI	RAATTY	6 cut(s)	321, 480, 824, 1247, 1430, 2285
XceI	RCATGY	2 cut(s)	689, 2519
XcmI	CCANNNNNNNNNTGG	2 cut(s)	241, 343
XmaI	CCCGGG	1 cut(s)	1494
XmiI	GTMKAC	1 cut(s)	850
XmnI	GAANNNNTTC	1 cut(s)	1316
XspI	CTAG	2 cut(s)	809, 866

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.