RLG00000027633

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001300
Physical Location & Seq
Forward (+)
13345886 .. 13359664
13779 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027633

Sequence Viewer

Length: 2280 bp
ATGGCAGTGGTGCGTGCTCTCACCCTCAGATCGACCCTGGCGGAGGCGCCACCTCCACAGTTGAGGTCGGGGTTTGGTACTATTGGTGAACGGGTCCAGGTTTTCGCCAATCACTTCAGCGTGGTGGTTGAGGAAGGTCATGTTTCAATTACACCCGCATGCTTGTCAAAGAGGATAAACATAGCGGTGATCGACGAGCTGGTTTACATGTACCCATATGATGGCATGAAGAGTCTCTACACCGCTCTGCCATATCCCTTCTCTTCGAAAGAGTTTGTGGTCAAGCTGTGGGGATCTCCATTCCAATGGAAAGAAAGAAAGTTCAAGGTCTACATAAAGTTGGGAAAAAAGTATTATAAGCAACACGAAACACCACAAAAGATCATCCAAGCTCTTGATGTTGCTCTTAAAACTCAAGCATCAAATACAAATTGTTTTCGTATGCATATTTGTTGGCCTTGTATGTCACTTTTTTTCATTTTTGGTACTGGTGGTGATATCGATGGTGGCTTCGAGTTATGGAGAGGCTACTACCAAAGCCTAAGGCCAACTCAGTTTGGTCTATCCCTCAATATTGATGTCTCGACAAAGGCTTTTTACCAGTCGACTCTGGTGACAGAGTTTGTTCAGCAACATTTTAACTACAGAGATATCTCCAAAGGTCTATCCAATCGTGATTCTGGAAAAAAAAACCTGAAAGGAGTCAAGGTTTACCTGACCTATGCACCCTACCAAACATATCGGATTAGTGGAGTGACTGCACAACCACTAAGGGAGTTGACGTTTATTCTTGAGGAGGACAAACAAACTAAAGTATCTGTGATTCGATATTATCATGATAAGTACGGTATTAACCTAAAACATGTTGCTTTGCCTGCCCTTCAATGTGGCAATGATTCAAGGCTTGTTTATTTGCCCATGGAGCTGTCTGTTATTATTCGTGGGCAAAGATACGCAGGGAAACTTAATAAAAAGCAAGTAACAAAGCTGCTAAGAGCAACTTGCTCAAAGCCCACGGCTAGGGGAAAAGAGATAAAGATGGTGGCCGATGATATTCATTACCACCAAAACTTAATGAACAGTCAATTTGGAATAAAAGTGAGCAAAGACATGGAACTTGTGGATGCTAGGGTATTACCACCTCCAATGCTTAAATACCATGACAGTTGTCTTGAAACAAAGAATACTCCCCGGATGGGTGCGTGGAACATGGACAATAAGATGTTCAATTCCAAGCCTTTATTTCCTATGCTACCACGTCATCGTGGGCATATTGAGACGGTTCTCAGATATATTCACAAGCGGTCTATCCATACACTTGAGAAGATTGGACACAGCAACAGACACCTTCAGTTGCTGATTATCGTTTTGGCTGATGAAAGATGTGGTAAGCAATACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGGTTGGTGGCAGAAACACCGTCTTAAATGATGCCGTTCAAAGGAGCATTCCTCATGTCAGTGATGATCCTACAATCATAATCAGTGCTGACGTCGCACATCCACAACCTAGGGAAGACAGTAGCCCATCCATAGCAGCAGTGGTTGCCTCAATGGATTTGCCGGAGGTCTATCAGTATAGAGGAGTCTTATCACAGCAGAATCATCATGAAGAAATCATCCAAGATCTTTATAAATCAAAAACTCGGGATTCTGGAATAATCAGGTATGGAAAACTTTTCTGTGCATTCCAAACTTCGACTGGCCAGAAGTCACGCAGAATCATTTTCTACAGGGATGGTGTCGATGAAGGGCAGCTTAGAATTCTTGCCTGTGCTTCTTTGGATGAGGGGTATATGCCTCCAGTTACTTTTGTTGTGGTACAAAAAAGGCATCACACTCGCCTTTTTCTTTCTGGCGACAAGAGCCATAGGAAATGCAATGTTCTACCAGGTACCGTGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTACCTTACTAGCCTTGATGCAATTCAGGGAACTAGTTGCCCTACTCACTATCATGGCCTGTTTGATGAGAACAAGTTCAGTGCTAATGCCTTGCAGATTCTCACCAACAATCTGTGCTACACCTATGCAAGGTGCACCCGTGCAGTTTCCGTAGTGCCTCCTGTCCATTACGCTCATCTGGCTGCATCTAAAGGACGCTATTACATTAAGGGTGATGCCTCGGATGATGCTTCTTCAGGTGGCACTGCCGCACAATTCCAGGTACTTCCGGATGTGCATCATCATGTCAAGGATGTGATGTTTTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

760

Amino Acids

86.29

Weight (kDa)

9.34

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 31 - 136 1.7e-09 N-terminal domain of argonaute
ArgoL1 PF08699 164 - 201 1.7e-16 Argonaute linker 1 domain
PAZ PF02170 219 - 330 3.2e-24 PAZ domain
ArgoL2 PF16488 344 - 386 9.6e-11 Argonaute linker 2 domain
Piwi PF02171 460 - 726 8.4e-74 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 357, 1662
AatII GACGTC 1 cut(s) 1524
Acc65I GGTACC 1 cut(s) 1922
AccB1I GGYRCC 2 cut(s) 46, 1922
AccB7I CCANNNNNTGG 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 245
AccI GTMKAC 2 cut(s) 330, 605
AccIII TCCGGA 1 cut(s) 2236
AciI CCGC 6 cut(s) 41, 156, 185, 243, 1303, 2217
AclWI GGATC 2 cut(s) 301, 1490
AcoI YGGCCR 2 cut(s) 1044, 1732
AcsI RAATTY 2 cut(s) 1791, 1958
AcuI CTGAAG 3 cut(s) 100, 1334, 2187
AcyI GRCGYC 2 cut(s) 47, 1521
AfaI GTAC 7 cut(s) 79, 212, 487, 845, 1851, 1924, 2232
AfiI CCNNNNNNNGG 6 cut(s) 43, 221, 1020, 1196, 1470, 2097
AflII CTTAAG 1 cut(s) 1415
AflIII ACRYGT 2 cut(s) 207, 862
AgsI TTSAA 8 cut(s) 147, 325, 884, 900, 1175, 1228, 1403, 1469
AhlI ACTAGT 1 cut(s) 2000
AjiI CACGTC 1 cut(s) 1259
AjnI CCWGG 4 cut(s) 36, 96, 1918, 2226
AluBI AGCT 6 cut(s) 199, 286, 392, 925, 988, 1786
AluI AGCT 6 cut(s) 199, 286, 392, 925, 988, 1786
Alw21I GWGCWC 2 cut(s) 19, 2105
Alw26I GTCTC 4 cut(s) 239, 586, 1271, 1416
Alw44I GTGCAC 1 cut(s) 2101
AlwI GGATC 2 cut(s) 301, 1490
AlwNI CAGNNNCTG 1 cut(s) 1357
Ama87I CYCGRG 1 cut(s) 1674
Aor13HI TCCGGA 1 cut(s) 2236
AoxI GGCC 5 cut(s) 455, 545, 1044, 1732, 2023
ApaLI GTGCAC 1 cut(s) 2101
ApeKI GCWGC 4 cut(s) 988, 1565, 1783, 2150
ApoI RAATTY 2 cut(s) 1791, 1958
AseI ATTAAT 1 cut(s) 1422
Asp700I GAANNNNTTC 1 cut(s) 2042
Asp718I GGTACC 1 cut(s) 1922
AspA2I CCTAGG 1 cut(s) 1538
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 94
AsuC2I CCSGG 1 cut(s) 1192
AsuHPI GGTGA 7 cut(s) 13, 98, 199, 506, 625, 2062, 2192
AsuII TTCGAA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 1674
AvaII GGWCC 1 cut(s) 94
AvrII CCTAGG 1 cut(s) 1538
AxyI CCTNAGG 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 2105
BaeI ACNNNNGTAYC 4 cut(s) 798, 831, 1914, 1947
BalI TGGCCA 1 cut(s) 1734
BanI GGYRCC 2 cut(s) 46, 1922
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbsI GAAGAC 1 cut(s) 1551
Bbv12I GWGCWC 2 cut(s) 19, 2105
BbvI GCAGC 4 cut(s) 975, 1577, 1795, 2137
BccI CCATC 7 cut(s) 215, 497, 1033, 1189, 1564, 1760, 1956
BceAI ACGGC 2 cut(s) 1032, 1448
BcgI CGANNNNNNTGC 2 cut(s) 1850, 1884
BciT130I CCWGG 4 cut(s) 38, 98, 1920, 2228
BciVI GTATCC 1 cut(s) 1927
BcnI CCSGG 1 cut(s) 1192
BcoDI GTCTC 4 cut(s) 239, 586, 1271, 1416
BcuI ACTAGT 1 cut(s) 2000
BfaI CTAG 5 cut(s) 1020, 1128, 1539, 1977, 2001
BfmI CTRYAG 2 cut(s) 643, 1759
BfoI RGCGCY 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 1415
BfuI GTATCC 1 cut(s) 1927
BglII AGATCT 1 cut(s) 1654
BisI GCNGC 5 cut(s) 989, 1566, 1784, 2151, 2217
BlnI CCTAGG 1 cut(s) 1538
BlsI GCNGC 5 cut(s) 990, 1567, 1785, 2152, 2218
Bme1390I CCNGG 5 cut(s) 38, 98, 1192, 1920, 2228
Bme18I GGWCC 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 1674
BmgBI CACGTC 1 cut(s) 1259
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 3 cut(s) 48, 95, 1924
BmrFI CCNGG 5 cut(s) 38, 98, 1192, 1920, 2228
BmsI GCATC 9 cut(s) 428, 1114, 1450, 1870, 1975, 2162, 2173, 2185, 2254
BoxI GACNNNNGTC 1 cut(s) 1167
BpiI GAAGAC 1 cut(s) 1551
BplI GAGNNNNNCTC 2 cut(s) 1465, 1497
BpmI CTGGAG 1 cut(s) 1815
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 3 cut(s) 399, 812, 1340
BpuMI CCSGG 1 cut(s) 1192
Bsa29I ATCGAT 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 2244
BsaHI GRCGYC 2 cut(s) 47, 1521
BsaJI CCNNGG 7 cut(s) 36, 918, 1014, 1190, 1538, 1926, 2187
BsaWI WCCGGW 1 cut(s) 2236
BsaXI ACNNNNNCTCC 4 cut(s) 693, 723, 767, 797
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 221, 1020, 1196, 1470, 2097
Bse1I ACTGG 4 cut(s) 493, 601, 1735, 1832
Bse21I CCTNAGG 1 cut(s) 542
Bse3DI GCAATG 2 cut(s) 898, 1915
Bse8I GATNNNNATC 1 cut(s) 2244
BseAI TCCGGA 1 cut(s) 2236
BseBI CCWGG 4 cut(s) 38, 98, 1920, 2228
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 7 cut(s) 36, 918, 1014, 1190, 1538, 1926, 2187
BseJI GATNNNNATC 1 cut(s) 2244
BseLI CCNNNNNNNGG 6 cut(s) 43, 221, 1020, 1196, 1470, 2097
BseMI GCAATG 2 cut(s) 898, 1915
BseMII CTCAG 3 cut(s) 40, 566, 1300
BseNI ACTGG 4 cut(s) 493, 601, 1735, 1832
BseRI GAGGAG 2 cut(s) 809, 1626
BseSI GKGCMC 1 cut(s) 2105
BseXI GCAGC 4 cut(s) 975, 1577, 1795, 2137
BsgI GTGCAG 2 cut(s) 744, 2130
BshFI GGCC 5 cut(s) 457, 547, 1046, 1734, 2025
BshNI GGYRCC 2 cut(s) 46, 1922
BshVI ATCGAT 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 19, 2105
BsiHKCI CYCGRG 1 cut(s) 1674
BsiSI CCGG 3 cut(s) 1192, 1592, 2237
BslI CCNNNNNNNGG 6 cut(s) 43, 221, 1020, 1196, 1470, 2097
BsmAI GTCTC 4 cut(s) 239, 586, 1271, 1416
BsmBI CGTCTC 1 cut(s) 1271
BsmI GAATGC 2 cut(s) 1476, 1715
BsnI GGCC 5 cut(s) 457, 547, 1046, 1734, 2025
BsoBI CYCGRG 1 cut(s) 1674
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 2 cut(s) 19, 2105
Bsp13I TCCGGA 1 cut(s) 2236
Bsp143I GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 6 cut(s) 41, 156, 185, 243, 1303, 2217
BspANI GGCC 5 cut(s) 457, 547, 1046, 1734, 2025
BspCNI CTCAG 3 cut(s) 39, 565, 1299
BspDI ATCGAT 1 cut(s) 501
BspEI TCCGGA 1 cut(s) 2236
BspHI TCATGA 2 cut(s) 835, 1636
BspLI GGNNCC 3 cut(s) 48, 95, 1924
BspPI GGATC 2 cut(s) 301, 1490
BspT104I TTCGAA 1 cut(s) 266
BspT107I GGYRCC 2 cut(s) 46, 1922
BspTI CTTAAG 1 cut(s) 1415
BsrBI CCGCTC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 898, 1915
BsrI ACTGG 4 cut(s) 493, 601, 1735, 1832
BssECI CCNNGG 7 cut(s) 36, 918, 1014, 1190, 1538, 1926, 2187
BssMI GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
BssNI GRCGYC 2 cut(s) 47, 1521
BssT1I CCWWGG 2 cut(s) 918, 1538
Bst2UI CCWGG 4 cut(s) 38, 98, 1920, 2228
Bst4CI ACNGT 8 cut(s) 60, 848, 1082, 1166, 1282, 1450, 1550, 1927
Bst6I CTCTTC 2 cut(s) 224, 268
BstACI GRCGYC 2 cut(s) 47, 1521
BstAFI CTTAAG 1 cut(s) 1415
BstAPI GCANNNNNTGC 1 cut(s) 1574
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 15, 160, 876
BstDEI CTNAG 7 cut(s) 26, 542, 552, 770, 992, 1286, 1787
BstDSI CCRYGG 3 cut(s) 918, 1014, 1926
BstENI CCTNNNNNAGG 2 cut(s) 41, 2095
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 6 cut(s) 32, 192, 296, 384, 1498, 1657
BstMAI GTCTC 4 cut(s) 239, 586, 1271, 1416
BstMBI GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
BstMWI GCNNNNNNNGC 6 cut(s) 875, 922, 1523, 1574, 1893, 2147
BstNI CCWGG 4 cut(s) 38, 98, 1920, 2228
BstNSI RCATGY 3 cut(s) 162, 211, 866
BstPAI GACNNNNGTC 1 cut(s) 1167
BstSCI CCNGG 5 cut(s) 36, 96, 1190, 1918, 2226
BstSFI CTRYAG 2 cut(s) 643, 1759
BstSLI GKGCMC 1 cut(s) 2105
BstV1I GCAGC 4 cut(s) 975, 1577, 1795, 2137
BstV2I GAAGAC 1 cut(s) 1551
BstX2I RGATCY 2 cut(s) 293, 1654
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 2 cut(s) 293, 1654
Bsu15I ATCGAT 1 cut(s) 501
Bsu36I CCTNAGG 1 cut(s) 542
BsuI GTATCC 1 cut(s) 1927
BsuRI GGCC 5 cut(s) 457, 547, 1046, 1734, 2025
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 3 cut(s) 918, 1014, 1926
BtrI CACGTC 1 cut(s) 1259
BtsI GCAGTG 3 cut(s) 12, 1575, 2211
BtsIMutI CAGTG 6 cut(s) 12, 1495, 1519, 1575, 2053, 2211
Cac8I GCNNGC 3 cut(s) 15, 160, 876
CaiI CAGNNNCTG 1 cut(s) 1357
CciI TCATGA 2 cut(s) 835, 1636
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 94
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 1 cut(s) 2172
CsiI ACCWGGT 1 cut(s) 1918
Csp6I GTAC 7 cut(s) 78, 211, 486, 844, 1850, 1923, 2231
CspCI CAANNNNNGTGG 2 cut(s) 1417, 1452
CviQI GTAC 7 cut(s) 78, 211, 486, 844, 1850, 1923, 2231
DdeI CTNAG 7 cut(s) 26, 542, 552, 770, 992, 1286, 1787
DinI GGCGCC 1 cut(s) 48
DpnI GATC 6 cut(s) 31, 191, 295, 383, 1497, 1656
DpnII GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
EaeI YGGCCR 2 cut(s) 1044, 1732
Eam1104I CTCTTC 2 cut(s) 224, 268
EarI CTCTTC 2 cut(s) 224, 268
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 918, 1538
Eco32I GATATC 2 cut(s) 499, 652
Eco47I GGWCC 1 cut(s) 94
Eco57I CTGAAG 3 cut(s) 100, 1334, 2187
Eco81I CCTNAGG 1 cut(s) 542
Eco88I CYCGRG 1 cut(s) 1674
EcoNI CCTNNNNNAGG 2 cut(s) 41, 2095
EcoRI GAATTC 1 cut(s) 1791
EcoRII CCWGG 4 cut(s) 36, 96, 1918, 2226
EcoRV GATATC 2 cut(s) 499, 652
EcoT14I CCWWGG 2 cut(s) 918, 1538
EcoT22I ATGCAT 1 cut(s) 447
EgeI GGCGCC 1 cut(s) 48
EheI GGCGCC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 918, 1538
Esp3I CGTCTC 1 cut(s) 1271
FalI AAGNNNNNCTT 4 cut(s) 985, 1017, 1770, 1802
FauI CCCGC 1 cut(s) 163
FauNDI CATATG 1 cut(s) 217
FblI GTMKAC 2 cut(s) 330, 605
Fnu4HI GCNGC 5 cut(s) 989, 1566, 1784, 2151, 2217
Fsp4HI GCNGC 5 cut(s) 989, 1566, 1784, 2151, 2217
FspBI CTAG 5 cut(s) 1020, 1128, 1539, 1977, 2001
GlaI GCGC 1 cut(s) 48
GluI GCNGC 5 cut(s) 989, 1566, 1784, 2151, 2217
GsuI CTGGAG 1 cut(s) 1815
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 5 cut(s) 457, 547, 1046, 1734, 2025
HapII CCGG 3 cut(s) 1192, 1592, 2237
HgaI GACGC 1 cut(s) 2172
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 2 cut(s) 47, 1521
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 2 cut(s) 606, 780
HindII GTYRAC 2 cut(s) 606, 780
HpaII CCGG 3 cut(s) 1192, 1592, 2237
HphI GGTGA 7 cut(s) 13, 98, 199, 506, 625, 2062, 2192
Hpy166II GTNNAC 7 cut(s) 89, 205, 331, 606, 712, 780, 2103
Hpy188I TCNGA 4 cut(s) 29, 744, 1289, 2191
Hpy8I GTNNAC 7 cut(s) 89, 205, 331, 606, 712, 780, 2103
Hpy99I CGWCG 2 cut(s) 197, 1526
HpyAV CCTTC 5 cut(s) 128, 268, 890, 1358, 1772
HpyCH4III ACNGT 8 cut(s) 60, 848, 1082, 1166, 1282, 1450, 1550, 1927
HpyCH4IV ACGT 3 cut(s) 782, 1258, 1521
HpyF10VI GCNNNNNNNGC 6 cut(s) 875, 922, 1523, 1574, 1893, 2147
HpyF3I CTNAG 7 cut(s) 26, 542, 552, 770, 992, 1286, 1787
HpySE526I ACGT 3 cut(s) 782, 1258, 1521
Hsp92I GRCGYC 2 cut(s) 47, 1521
HspAI GCGC 1 cut(s) 47
KasI GGCGCC 1 cut(s) 46
Kpn2I TCCGGA 1 cut(s) 2236
KpnI GGTACC 1 cut(s) 1926
Kzo9I GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
LmnI GCTCC 2 cut(s) 922, 1473
Lsp1109I GCAGC 4 cut(s) 975, 1577, 1795, 2137
LweI GCATC 9 cut(s) 428, 1114, 1450, 1870, 1975, 2162, 2173, 2185, 2254
MabI ACCWGGT 1 cut(s) 1918
MaeI CTAG 5 cut(s) 1020, 1128, 1539, 1977, 2001
MaeII ACGT 3 cut(s) 782, 1258, 1521
MaeIII GTNAC 6 cut(s) 465, 613, 754, 979, 1740, 1834
MalI GATC 6 cut(s) 31, 191, 295, 383, 1497, 1656
MbiI CCGCTC 1 cut(s) 245
MboI GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
MboII GAAGA 6 cut(s) 241, 255, 1336, 1556, 1652, 2193
MflI RGATCY 2 cut(s) 293, 1654
MhlI GDGCHC 2 cut(s) 19, 2105
MlsI TGGCCA 1 cut(s) 1734
MluCI AATT 8 cut(s) 147, 430, 1085, 1228, 1791, 1958, 1989, 2222
MluNI TGGCCA 1 cut(s) 1734
Mly113I GGCGCC 1 cut(s) 47
MlyI GAGTC 4 cut(s) 241, 601, 711, 1623
MmeI TCCRAC 1 cut(s) 1976
Mox20I TGGCCA 1 cut(s) 1734
Mph1103I ATGCAT 1 cut(s) 447
MroI TCCGGA 1 cut(s) 2236
MroXI GAANNNNTTC 1 cut(s) 2042
MscI TGGCCA 1 cut(s) 1734
MslI CAYNNNNRTG 6 cut(s) 157, 185, 304, 1488, 2019, 2250
Msp20I TGGCCA 1 cut(s) 1734
MspCI CTTAAG 1 cut(s) 1415
MspI CCGG 3 cut(s) 1192, 1592, 2237
MspR9I CCNGG 5 cut(s) 38, 98, 1192, 1920, 2228
Mva1269I GAATGC 2 cut(s) 1476, 1715
MvaI CCWGG 4 cut(s) 38, 98, 1920, 2228
MwoI GCNNNNNNNGC 6 cut(s) 875, 922, 1523, 1574, 1893, 2147
NarI GGCGCC 1 cut(s) 47
NciI CCSGG 1 cut(s) 1192
NcoI CCATGG 1 cut(s) 918
NdeI CATATG 1 cut(s) 217
NdeII GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
NlaIV GGNNCC 3 cut(s) 48, 95, 1924
NmuCI GTSAC 4 cut(s) 465, 613, 754, 1740
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 3 cut(s) 162, 211, 866
NspV TTCGAA 1 cut(s) 266
PaeI GCATGC 1 cut(s) 162
PagI TCATGA 2 cut(s) 835, 1636
PciI ACATGT 2 cut(s) 207, 862
PctI GAATGC 2 cut(s) 1476, 1715
PdmI GAANNNNTTC 1 cut(s) 2042
PfeI GAWTC 7 cut(s) 677, 823, 896, 1630, 1679, 1749, 2065
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 5 cut(s) 990, 1567, 1785, 2152, 2218
PleI GAGTC 4 cut(s) 240, 601, 710, 1622
PluTI GGCGCC 1 cut(s) 50
PpsI GAGTC 4 cut(s) 240, 601, 710, 1622
PscI ACATGT 2 cut(s) 207, 862
PshAI GACNNNNGTC 1 cut(s) 1167
PshBI ATTAAT 1 cut(s) 1422
PsiI TTATAA 2 cut(s) 357, 1662
Psp6I CCWGG 4 cut(s) 36, 96, 1918, 2226
PspGI CCWGG 4 cut(s) 36, 96, 1918, 2226
PspN4I GGNNCC 3 cut(s) 48, 95, 1924
PspPI GGNCC 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 1357
PsuI RGATCY 2 cut(s) 293, 1654
RsaI GTAC 7 cut(s) 79, 212, 487, 845, 1851, 1924, 2232
RsaNI GTAC 7 cut(s) 78, 211, 486, 844, 1850, 1923, 2231
RseI CAYNNNNRTG 6 cut(s) 157, 185, 304, 1488, 2019, 2250
SalI GTCGAC 1 cut(s) 604
SatI GCNGC 5 cut(s) 989, 1566, 1784, 2151, 2217
Sau3AI GATC 6 cut(s) 29, 189, 293, 381, 1495, 1654
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 4 cut(s) 241, 601, 711, 1623
ScrFI CCNGG 5 cut(s) 38, 98, 1192, 1920, 2228
SduI GDGCHC 2 cut(s) 19, 2105
SexAI ACCWGGT 1 cut(s) 1918
SfaNI GCATC 9 cut(s) 428, 1114, 1450, 1870, 1975, 2162, 2173, 2185, 2254
SfcI CTRYAG 2 cut(s) 643, 1759
SfoI GGCGCC 1 cut(s) 48
SfuI TTCGAA 1 cut(s) 266
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 6 cut(s) 157, 185, 304, 1488, 2019, 2250
SmlI CTYRAG 4 cut(s) 414, 791, 1319, 1415
SmoI CTYRAG 4 cut(s) 414, 791, 1319, 1415
SpeI ACTAGT 1 cut(s) 2000
SphI GCATGC 1 cut(s) 162
Sse9I AATT 8 cut(s) 147, 430, 1085, 1228, 1791, 1958, 1989, 2222
SsiI CCGC 6 cut(s) 41, 156, 185, 243, 1303, 2217
SspDI GGCGCC 1 cut(s) 46
SspI AATATT 1 cut(s) 574
SspMI CTAG 5 cut(s) 1020, 1128, 1539, 1977, 2001
StyD4I CCNGG 5 cut(s) 36, 96, 1190, 1918, 2226
StyI CCWWGG 2 cut(s) 918, 1538
TaaI ACNGT 8 cut(s) 60, 848, 1082, 1166, 1282, 1450, 1550, 1927
TaiI ACGT 3 cut(s) 785, 1261, 1524
TasI AATT 8 cut(s) 147, 430, 1085, 1228, 1791, 1958, 1989, 2222
TauI GCSGC 1 cut(s) 2219
TfiI GAWTC 7 cut(s) 677, 823, 896, 1630, 1679, 1749, 2065
TscAI CASTG 6 cut(s) 12, 1495, 1519, 1575, 2053, 2218
TseFI GTSAC 4 cut(s) 465, 613, 754, 1740
TseI GCWGC 4 cut(s) 988, 1565, 1783, 2150
Tsp45I GTSAC 4 cut(s) 465, 613, 754, 1740
TspDTI ATGAA 7 cut(s) 242, 466, 1046, 1091, 1392, 1653, 1791
TspGWI ACGGA 1 cut(s) 2107
TspRI CASTG 6 cut(s) 12, 1495, 1519, 1575, 2053, 2218
Van91I CCANNNNNTGG 1 cut(s) 221
Vha464I CTTAAG 1 cut(s) 1415
VneI GTGCAC 1 cut(s) 2101
VpaK11BI GGWCC 1 cut(s) 94
VspI ATTAAT 1 cut(s) 1422
XagI CCTNNNNNAGG 2 cut(s) 41, 2095
XapI RAATTY 2 cut(s) 1791, 1958
XceI RCATGY 3 cut(s) 162, 211, 866
XcmI CCANNNNNNNNNTGG 2 cut(s) 1567, 1727
XmaJI CCTAGG 1 cut(s) 1538
XmiI GTMKAC 2 cut(s) 330, 605
XmnI GAANNNNTTC 1 cut(s) 2042
XspI CTAG 5 cut(s) 1020, 1128, 1539, 1977, 2001
ZraI GACGTC 1 cut(s) 1522
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.