RchiOBHm_Chr5g0056091

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001300
Physical Location & Seq
Reverse (-)
59501744 .. 59507869
6126 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33302

Sequence Viewer

Length: 2949 bp
ATGTCCAATCGCGGCGGTTCATCCGGTAGTCGCGGCGGCGGAGGCCGCGGCCGCGGAAGAGGAAATCCCGGCGGTCAGGCCAGAGGCTCAGGCTACACTCCGGCGCCCGCGCCCGCTCCGGCCCCTGCTCCAGCTCCTGCTCCTGCTCCGGCGTATGCTCCGGCGCCGGCTCGTGCTCAGCCGCCTGCTGTTGCCTCGTCGAGCACGGTACCGGCGCCGGCTCGTACTCATCAGCCTGCTGTGGCCTCGTCAAGCAGGGCTCCGGCTCCGGCTCCGGCTCCGGCGCCGGCACCTGTTTCATCGGCGGCGGCGGCTTCGCTGGCGAGTGAGATGGAAGAGAAGCTTGCATTGGCTCCGTCGTCATCCAAGGCCGTGAGGTTCCCAGTGAGGCCGGGGTTTGGTAGAGCTGGTCAAAGAGTCCAAGTCCGAGCCAATCATTTTCTGGTCGAGATTGGGGAAAGAGATCTGCATCACTATGATGTATCAATTAGCCCTGAAGTTGCATCAAAGAAGATAAACAGAGATGTAATCAACCAGCTGGTTCAGATGTACTCTGAGTCTCACTTGGGAAAGAGAATGCCAGCATATGATGGCATGAAGAGTATCTACACTGCTGGGTCATTGCCCTTCTCTTCAAAGGAGTTTGTGGTCAAGCTACCTGAGAGAGAAGGTCGAGCTGGATCTGCATCAAGAAGGAGAGATAGAGACTTCAAGGTCTGTATCAAGTTGGCAAACAAGCCTGACCTGTATCAACTGCAGCAGTTCTTACGCGGAAGACTACATGAAACTCCACAAGAGACCATCCAAGTGCTTGATGTTGTTCTTAGAGCTGCACCATCACAGAAATACTGTGTGGTTGGTAGGTCATTTTTTAGCCCTGATCTGGGTCCTCGAGGTGACCTTGAATTTGGCTTGGAATACTGGAGAGGCTTTTACCAAAGCCTAAGGCCAACCCAGTGTGGTCTATCCCTAAATATTGATATCTCAGCAAGGGCTTTTTATGATCCGATTCTGGTGACAGAGTTTATTAAGAAACATTTTGGGTACAGAGATTTATCCAGGGGTCTGTCGGATCGTGATTGTCTGAAGCTGAAGAAAAATCTGAAAGGAGTCAAGGTAACTATGAGCTGTGGAGGCAGCAGAAGTTATCGAATTACTGGAGTATCTGTGCAGCCTCTAAGCCAGTTAACGTTCACTGTTGAGGATAATCAAACCAGAATATCAGTGGTTCAGTATTATCGTGAGAAGTACAACATTAGCCTAAAACAGGTGGCTTTGCCTGCCCTTCAATCTGGCAATGATTCCAGGCCTGCTTATTTGCCCATGGAGCTCGCTACAATTGTTCCTGGGCAGAGATACACGAAAAGACTCAATGAAAAGCAAGTGACAAACGTGCTGAGAGCAACTTGCCAACGGCCCCGGGAGAGGGAATCCAATATACTGAAGATGGTGATGCACAATAATTACAAGGGAGAGGATCTTGTAAACAAAGAATTTGGAATGCAAGTGAAAGAACAAATGGCACTGGTGGATGCTAGAGTATTACCACCTCCAATGCTTAAGTATCATGAGAGTGGTCGTGAAACAAAGGATACTCCGAGGATGGGTGCATGGAACATGATCAATAAGAAAATGGTTAATGGTGCCAAAGTGGATTTCTGGGCTTTTGTCAATTTCTCTCGATCACGTGAAGATTTTAACTATCGCTTTTGTGAGGATCTCGTCTATATGTGCAACAGTAAAGGGGTGGAGTTCCATTCCGAGCCTTTACTTCCTGTACAGTCAGCTCATCCTAATCAAATTGAGAGGGTTCTGAGAGATATTCACAAGCAGTCAGTCCAGAGACTTAATGAGATGGGACAAGCCAGCAAACATCTTCAGTTGTTGATTATCATCCTGCCTGATGTCAAAGGATCTTATGGTCCCATTAAAAGAATTTGTGAAACAGAGCTTGGAATTGTCTCCCAGTGCTGTAATCCTAAGCAAGCACAAAAGCTTAGTAAGCAATACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGGTTGGTGGTAGAAACACAGTCTTAAATGATGCCATTCAGAGGAGGATTCCTCTCGTCAGTGATCGTCCTACAATCATCCTTGGAGCTGATGTCACTCATCCACAAGCTGGGGAAGACAGTAGCCCATCTATAGCAGCAGTGGTTGCCTCAATGGACTGGCCAGAGGTCTCCAAGTATAGAGGAATCCTGTCAGCACAGAATCATCGTCAAGAAATCATCCAAGATCTTTACAAATTAACTCATGATGAGAAAAGAGGGCTGGTTCATTCAGGAATGATCAGAGAACTTTTCATTGCATTCCGAAGATCGACTGGCCAAAAACCGCACAGAATCATCTTCTATAGGGATGGGGTGAGTGAAGGGCAGTTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACGGAAGGCCTGTGCTTCTTTGGAGGAGGGGTATATGCCACCAGTTACTTTTGTTGTGGTGCAGAAAAGGCATCACACACGCCTTTTCCCTGCTGACAACCAGACAGATAGGAGTGGGAATATTCAACCAGGTACTGTGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTATCTTAATAGCCATGCAGGAATTCAGGGAACAAGTCGCCCAGCTCATTATCATGTCCTGTTTGATGAGAACAACTTCACTGCTGATGGCTTGCAGATTCTCACCAACAATCTGTGCTACACATATGCAAGGTGCACCCGCTCAGTTTCCATAGTGCCTCCTGCCTATTATGCGCATCTAGCTGCTTTTAGAGCACGCTATTACATTGAGGGCGATTCATCAGATGGTGCTTCTTCAGTTGGCAGAGGCAGCGGTGGCAGTGCTGCACAAACCCAGGTCCTTCCAAATGTGCACGAGTATGTCAAGGATGTGATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

982

Amino Acids

108.98

Weight (kDa)

9.53

Isoelectric Point (pI)

54.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 138 - 275 3.5e-37 N-terminal domain of argonaute
ArgoL1 PF08699 286 - 334 4.3e-19 Argonaute linker 1 domain
PAZ PF02170 362 - 465 4.2e-26 PAZ domain
ArgoL2 PF16488 475 - 522 2.4e-13 Argonaute linker 2 domain
ArgoMid PF16487 532 - 610 4.9e-09 Mid domain of argonaute
Piwi PF02171 629 - 943 7.2e-108 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 713
AbsI CCTCGAGG 1 cut(s) 891
Acc16I TGCGCA 1 cut(s) 2796
Acc65I GGTACC 1 cut(s) 208
AccB1I GGYRCC 7 cut(s) 103, 163, 208, 214, 283, 289, 1643
AccB7I CCANNNNNTGG 1 cut(s) 2150
AccBSI CCGCTC 2 cut(s) 116, 2763
AccII CGCG 6 cut(s) 12, 33, 48, 54, 110, 771
AclI AACGTT 1 cut(s) 1190
AclWI GGATC 6 cut(s) 688, 998, 1080, 1485, 1725, 1921
AcoI YGGCCR 3 cut(s) 49, 2201, 2356
AcsI RAATTY 5 cut(s) 905, 1493, 1935, 2612, 2643
AcuI CTGAAG 6 cut(s) 516, 1106, 1112, 1463, 1862, 2841
AcvI CACGTG 1 cut(s) 1688
AcyI GRCGYC 4 cut(s) 104, 164, 215, 284
AfaI GTAC 7 cut(s) 210, 226, 551, 1046, 1250, 1779, 2578
AfiI CCNNNNNNNGG 9 cut(s) 398, 883, 884, 1267, 1425, 1426, 1604, 2082, 2150
AflII CTTAAG 2 cut(s) 1559, 2027
AgsI TTSAA 6 cut(s) 636, 712, 905, 1289, 2015, 2570
AjnI CCWGG 5 cut(s) 1058, 1304, 1345, 2572, 2895
AjuI GAANNNNNNNTTGG 6 cut(s) 332, 364, 1640, 1672, 1935, 1967
Alw21I GWGCWC 6 cut(s) 178, 206, 1332, 2759, 2818, 2916
Alw26I GTCTC 7 cut(s) 564, 699, 791, 1837, 1966, 2028, 2215
Alw44I GTGCAC 2 cut(s) 2755, 2912
AlwI GGATC 6 cut(s) 688, 998, 1080, 1485, 1725, 1921
AlwNI CAGNNNCTG 2 cut(s) 137, 2579
Ama87I CYCGRG 2 cut(s) 891, 1419
ApaLI GTGCAC 2 cut(s) 2755, 2912
ApeKI GCWGC 8 cut(s) 757, 830, 1137, 1171, 2177, 2804, 2871, 2885
ApoI RAATTY 5 cut(s) 905, 1493, 1935, 2612, 2643
ArsI GACNNNNNNTTYG 2 cut(s) 890, 922
AseI ATTAAT 1 cut(s) 2034
Asp700I GAANNNNTTC 1 cut(s) 2696
Asp718I GGTACC 1 cut(s) 208
AspLEI GCGC 6 cut(s) 106, 112, 166, 217, 286, 2797
AspS9I GGNCC 5 cut(s) 121, 887, 1416, 1922, 2899
AsuC2I CCSGG 4 cut(s) 69, 393, 1420, 1421
AsuHPI GGTGA 5 cut(s) 908, 1027, 1462, 2407, 2716
AvaI CYCGRG 2 cut(s) 891, 1419
AvaII GGWCC 3 cut(s) 887, 1922, 2899
AxyI CCTNAGG 1 cut(s) 944
BaeGI GKGCMC 2 cut(s) 2759, 2916
BaeI ACNNNNGTAYC 4 cut(s) 1146, 1179, 2568, 2601
BalI TGGCCA 2 cut(s) 2203, 2358
BanI GGYRCC 7 cut(s) 103, 163, 208, 214, 283, 289, 1643
BanII GRGCYC 2 cut(s) 262, 1332
BarI GAAGNNNNNNTAC 2 cut(s) 590, 622
BauI CACGAG 2 cut(s) 171, 2915
BbrPI CACGTG 1 cut(s) 1688
BbsI GAAGAC 2 cut(s) 781, 2163
Bbv12I GWGCWC 6 cut(s) 178, 206, 1332, 2759, 2818, 2916
BbvI GCAGC 8 cut(s) 769, 817, 1149, 1183, 2189, 2791, 2872, 2883
BceAI ACGGC 2 cut(s) 356, 1430
BciT130I CCWGG 5 cut(s) 1060, 1306, 1347, 2574, 2897
BciVI GTATCC 2 cut(s) 1585, 2581
BclI TGATCA 2 cut(s) 1620, 2319
BcnI CCSGG 4 cut(s) 69, 393, 1420, 1421
BcoDI GTCTC 7 cut(s) 564, 699, 791, 1837, 1966, 2028, 2215
BfaI CTAG 2 cut(s) 1536, 2801
BfmI CTRYAG 3 cut(s) 755, 2172, 2383
BfoI RGCGCY 4 cut(s) 107, 167, 218, 287
BfrI CTTAAG 2 cut(s) 1559, 2027
BfuI GTATCC 2 cut(s) 1585, 2581
BglII AGATCT 2 cut(s) 463, 2266
BlpI GCTNAGC 1 cut(s) 177
Bme1390I CCNGG 9 cut(s) 69, 393, 1060, 1306, 1347, 1420, 1421, 2574, 2897
Bme18I GGWCC 3 cut(s) 887, 1922, 2899
BmeT110I CYCGRG 2 cut(s) 891, 1419
BmgT120I GGNCC 5 cut(s) 121, 887, 1416, 1922, 2899
BmrFI CCNGG 9 cut(s) 69, 393, 1060, 1306, 1347, 1420, 1421, 2574, 2897
BmrI ACTGGG 3 cut(s) 377, 949, 1960
BmsI GCATC 9 cut(s) 478, 512, 695, 1443, 1522, 2062, 2428, 2524, 2806
BmuI ACTGGG 3 cut(s) 377, 949, 1960
BpiI GAAGAC 2 cut(s) 781, 2163
BplI GAGNNNNNCTC 2 cut(s) 2077, 2109
BpmI CTGGAG 3 cut(s) 114, 943, 1179
Bpu10I CCTNAGC 2 cut(s) 88, 1980
Bpu1102I GCTNAGC 1 cut(s) 177
BpuMI CCSGG 4 cut(s) 69, 393, 1420, 1421
BsaAI YACGTR 1 cut(s) 1688
BsaBI GATNNNNATC 4 cut(s) 468, 685, 1892, 2622
BsaHI GRCGYC 4 cut(s) 104, 164, 215, 284
BsaI GGTCTC 2 cut(s) 791, 2215
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 8 cut(s) 1101, 1131, 1152, 1182, 2118, 2148, 2459, 2489
Bsc4I CCNNNNNNNGG 9 cut(s) 398, 883, 884, 1267, 1425, 1426, 1604, 2082, 2150
Bse118I RCCGGY 4 cut(s) 166, 211, 217, 286
Bse21I CCTNAGG 1 cut(s) 944
Bse3DI GCAATG 3 cut(s) 620, 1303, 2334
Bse8I GATNNNNATC 4 cut(s) 468, 685, 1892, 2622
BseBI CCWGG 5 cut(s) 1060, 1306, 1347, 2574, 2897
BseJI GATNNNNATC 4 cut(s) 468, 685, 1892, 2622
BseLI CCNNNNNNNGG 9 cut(s) 398, 883, 884, 1267, 1425, 1426, 1604, 2082, 2150
BseMI GCAATG 3 cut(s) 620, 1303, 2334
BseMII CTCAG 8 cut(s) 102, 191, 546, 651, 999, 1388, 1805, 2778
BseRI GAGGAG 2 cut(s) 2098, 2483
BseSI GKGCMC 2 cut(s) 2759, 2916
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 8 cut(s) 769, 817, 1149, 1183, 2189, 2791, 2872, 2883
BseYI CCCAGC 3 cut(s) 614, 2150, 2662
BsgI GTGCAG 4 cut(s) 816, 1190, 2525, 2871
Bsh1236I CGCG 6 cut(s) 12, 33, 48, 54, 110, 771
Bsh1285I CGRYCG 1 cut(s) 52
BshNI GGYRCC 7 cut(s) 103, 163, 208, 214, 283, 289, 1643
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 6 cut(s) 178, 206, 1332, 2759, 2818, 2916
BsiHKCI CYCGRG 2 cut(s) 891, 1419
BslFI GGGAC 2 cut(s) 1872, 1908
BslI CCNNNNNNNGG 9 cut(s) 398, 883, 884, 1267, 1425, 1426, 1604, 2082, 2150
BsmAI GTCTC 7 cut(s) 564, 699, 791, 1837, 1966, 2028, 2215
BsmFI GGGAC 2 cut(s) 1872, 1908
BsmI GAATGC 3 cut(s) 582, 1506, 2339
Bso31I GGTCTC 2 cut(s) 791, 2215
BsoBI CYCGRG 2 cut(s) 891, 1419
Bsp1286I GDGCHC 7 cut(s) 178, 206, 262, 1332, 2759, 2818, 2916
Bsp1407I TGTACA 1 cut(s) 1777
Bsp1720I GCTNAGC 1 cut(s) 177
Bsp19I CCATGG 1 cut(s) 1323
BspCNI CTCAG 8 cut(s) 101, 190, 547, 652, 998, 1389, 1806, 2777
BspFNI CGCG 6 cut(s) 12, 33, 48, 54, 110, 771
BspHI TCATGA 2 cut(s) 1567, 2284
BspMAI CTGCAG 1 cut(s) 759
BspPI GGATC 6 cut(s) 688, 998, 1080, 1485, 1725, 1921
BspT107I GGYRCC 7 cut(s) 103, 163, 208, 214, 283, 289, 1643
BspTI CTTAAG 2 cut(s) 1559, 2027
BspTNI GGTCTC 2 cut(s) 791, 2215
BsrBI CCGCTC 2 cut(s) 116, 2763
BsrDI GCAATG 3 cut(s) 620, 1303, 2334
BsrFI RCCGGY 4 cut(s) 166, 211, 217, 286
BsrGI TGTACA 1 cut(s) 1777
BssAI RCCGGY 4 cut(s) 166, 211, 217, 286
BssNI GRCGYC 4 cut(s) 104, 164, 215, 284
BssSI CACGAG 2 cut(s) 171, 2915
BssT1I CCWWGG 3 cut(s) 366, 1323, 2122
Bst2BI CACGAG 2 cut(s) 171, 2915
Bst2UI CCWGG 5 cut(s) 1060, 1306, 1347, 2574, 2897
Bst4CI ACNGT 8 cut(s) 208, 851, 1198, 1739, 1782, 2062, 2162, 2581
Bst6I CTCTTC 4 cut(s) 52, 330, 593, 637
BstACI GRCGYC 4 cut(s) 104, 164, 215, 284
BstAFI CTTAAG 2 cut(s) 1559, 2027
BstAPI GCANNNNNTGC 1 cut(s) 2186
BstAUI TGTACA 1 cut(s) 1777
BstBAI YACGTR 1 cut(s) 1688
BstDSI CCRYGG 3 cut(s) 46, 52, 1323
BstEII GGTNACC 1 cut(s) 896
BstENI CCTNNNNNAGG 1 cut(s) 1265
BstFNI CGCG 6 cut(s) 12, 33, 48, 54, 110, 771
BstH2I RGCGCY 4 cut(s) 107, 167, 218, 287
BstHHI GCGC 6 cut(s) 106, 112, 166, 217, 286, 2797
BstMAI GTCTC 7 cut(s) 564, 699, 791, 1837, 1966, 2028, 2215
BstMCI CGRYCG 1 cut(s) 52
BstNI CCWGG 5 cut(s) 1060, 1306, 1347, 2574, 2897
BstPI GGTNACC 1 cut(s) 896
BstSCI CCNGG 9 cut(s) 67, 391, 1058, 1304, 1345, 1418, 1419, 2572, 2895
BstSFI CTRYAG 3 cut(s) 755, 2172, 2383
BstSLI GKGCMC 2 cut(s) 2759, 2916
BstUI CGCG 6 cut(s) 12, 33, 48, 54, 110, 771
BstV1I GCAGC 8 cut(s) 769, 817, 1149, 1183, 2189, 2791, 2872, 2883
BstV2I GAAGAC 2 cut(s) 781, 2163
BstX2I RGATCY 6 cut(s) 463, 680, 1477, 1717, 1913, 2266
BstYI RGATCY 6 cut(s) 463, 680, 1477, 1717, 1913, 2266
BstZI CGGCCG 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 944
BsuI GTATCC 2 cut(s) 1585, 2581
BtgI CCRYGG 3 cut(s) 46, 52, 1323
BtsI GCAGTG 4 cut(s) 609, 2187, 2700, 2887
CaiI CAGNNNCTG 2 cut(s) 137, 2579
CciI TCATGA 2 cut(s) 1567, 2284
CciNI GCGGCCGC 1 cut(s) 49
CfoI GCGC 6 cut(s) 106, 112, 166, 217, 286, 2797
Cfr10I RCCGGY 4 cut(s) 166, 211, 217, 286
Cfr13I GGNCC 5 cut(s) 121, 887, 1416, 1922, 2899
Cfr42I CCGCGG 2 cut(s) 49, 55
Cfr9I CCCGGG 1 cut(s) 1419
CsiI ACCWGGT 1 cut(s) 2572
Csp6I GTAC 7 cut(s) 209, 225, 550, 1045, 1249, 1778, 2577
CspCI CAANNNNNGTGG 2 cut(s) 2029, 2064
CviAII CATG 9 cut(s) 595, 782, 1324, 1568, 1611, 1618, 2285, 2636, 2675
CviQI GTAC 7 cut(s) 209, 225, 550, 1045, 1249, 1778, 2577
DinI GGCGCC 4 cut(s) 105, 165, 216, 285
DrdI GACNNNNNNGTC 1 cut(s) 713
DseDI GACNNNNNNGTC 1 cut(s) 713
EaeI YGGCCR 3 cut(s) 49, 2201, 2356
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 4 cut(s) 52, 330, 593, 637
EarI CTCTTC 4 cut(s) 52, 330, 593, 637
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 1330
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 366, 1323, 2122
Eco147I AGGCCT 2 cut(s) 1309, 2453
Eco24I GRGCYC 2 cut(s) 262, 1332
Eco31I GGTCTC 2 cut(s) 791, 2215
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 3 cut(s) 887, 1922, 2899
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 1330
Eco57I CTGAAG 6 cut(s) 516, 1106, 1112, 1463, 1862, 2841
Eco72I CACGTG 1 cut(s) 1688
Eco81I CCTNAGG 1 cut(s) 944
Eco88I CYCGRG 2 cut(s) 891, 1419
Eco91I GGTNACC 1 cut(s) 896
EcoICRI GAGCTC 1 cut(s) 1330
EcoNI CCTNNNNNAGG 1 cut(s) 1265
EcoO109I RGGNCCY 2 cut(s) 887, 2899
EcoO65I GGTNACC 1 cut(s) 896
EcoRI GAATTC 1 cut(s) 2643
EcoRII CCWGG 5 cut(s) 1058, 1304, 1345, 2572, 2895
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 3 cut(s) 366, 1323, 2122
EcoT38I GRGCYC 2 cut(s) 262, 1332
EgeI GGCGCC 4 cut(s) 105, 165, 216, 285
EheI GGCGCC 4 cut(s) 105, 165, 216, 285
ErhI CCWWGG 3 cut(s) 366, 1323, 2122
FaeI CATG 9 cut(s) 598, 785, 1327, 1571, 1614, 1621, 2288, 2639, 2678
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 2 cut(s) 1872, 1908
FatI CATG 9 cut(s) 594, 781, 1323, 1567, 1610, 1617, 2284, 2635, 2674
FauI CCCGC 3 cut(s) 115, 121, 2768
FauNDI CATATG 2 cut(s) 586, 2746
FbaI TGATCA 2 cut(s) 1620, 2319
FriOI GRGCYC 2 cut(s) 262, 1332
FspAI RTGCGCAY 1 cut(s) 2796
FspBI CTAG 2 cut(s) 1536, 2801
FspI TGCGCA 1 cut(s) 2796
GlaI GCGC 6 cut(s) 105, 111, 165, 216, 285, 2796
GsaI CCCAGC 3 cut(s) 618, 2154, 2666
GsuI CTGGAG 3 cut(s) 114, 943, 1179
HaeII RGCGCY 4 cut(s) 107, 167, 218, 287
HhaI GCGC 6 cut(s) 106, 112, 166, 217, 286, 2797
Hin1I GRCGYC 4 cut(s) 104, 164, 215, 284
Hin1II CATG 9 cut(s) 598, 785, 1327, 1571, 1614, 1621, 2288, 2639, 2678
Hin6I GCGC 6 cut(s) 104, 110, 164, 215, 284, 2795
HinP1I GCGC 6 cut(s) 104, 110, 164, 215, 284, 2795
HincII GTYRAC 1 cut(s) 1188
HindII GTYRAC 1 cut(s) 1188
HindIII AAGCTT 2 cut(s) 341, 1994
HpaI GTTAAC 1 cut(s) 1188
HphI GGTGA 5 cut(s) 908, 1027, 1462, 2407, 2716
Hpy166II GTNNAC 5 cut(s) 1188, 1194, 1486, 2757, 2914
Hpy8I GTNNAC 5 cut(s) 1188, 1194, 1486, 2757, 2914
Hpy99I CGWCG 2 cut(s) 202, 361
HpyAV CCTTC 7 cut(s) 637, 662, 687, 1295, 2396, 2443, 2912
HpyCH4III ACNGT 8 cut(s) 208, 851, 1198, 1739, 1782, 2062, 2162, 2581
HpyCH4IV ACGT 3 cut(s) 1190, 1392, 1687
HpySE526I ACGT 3 cut(s) 1190, 1392, 1687
Hsp92I GRCGYC 4 cut(s) 104, 164, 215, 284
Hsp92II CATG 9 cut(s) 598, 785, 1327, 1571, 1614, 1621, 2288, 2639, 2678
HspAI GCGC 6 cut(s) 104, 110, 164, 215, 284, 2795
KasI GGCGCC 4 cut(s) 103, 163, 214, 283
KpnI GGTACC 1 cut(s) 212
KroI GCCGGC 3 cut(s) 166, 217, 286
KroNI GCCGGC 3 cut(s) 168, 219, 288
Ksp22I TGATCA 2 cut(s) 1620, 2319
KspAI GTTAAC 1 cut(s) 1188
KspI CCGCGG 2 cut(s) 49, 55
Lsp1109I GCAGC 8 cut(s) 769, 817, 1149, 1183, 2189, 2791, 2872, 2883
LweI GCATC 9 cut(s) 478, 512, 695, 1443, 1522, 2062, 2428, 2524, 2806
MabI ACCWGGT 1 cut(s) 2572
MaeI CTAG 2 cut(s) 1536, 2801
MaeII ACGT 3 cut(s) 1190, 1392, 1687
MaeIII GTNAC 6 cut(s) 896, 1015, 1117, 1384, 2134, 2488
MbiI CCGCTC 2 cut(s) 116, 2763
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 6 cut(s) 463, 680, 1477, 1717, 1913, 2266
MhlI GDGCHC 7 cut(s) 178, 206, 262, 1332, 2759, 2818, 2916
MlsI TGGCCA 2 cut(s) 2203, 2358
MluNI TGGCCA 2 cut(s) 2203, 2358
Mly113I GGCGCC 4 cut(s) 104, 164, 215, 284
MlyI GAGTC 4 cut(s) 426, 566, 1119, 1362
MmeI TCCRAC 2 cut(s) 1050, 2630
Mox20I TGGCCA 2 cut(s) 2203, 2358
MroNI GCCGGC 3 cut(s) 166, 217, 286
MroXI GAANNNNTTC 1 cut(s) 2696
MscI TGGCCA 2 cut(s) 2203, 2358
MslI CAYNNNNRTG 6 cut(s) 474, 477, 806, 1572, 2673, 2919
Msp20I TGGCCA 2 cut(s) 2203, 2358
MspA1I CMGCKG 4 cut(s) 48, 54, 538, 2874
MspCI CTTAAG 2 cut(s) 1559, 2027
MspR9I CCNGG 9 cut(s) 69, 393, 1060, 1306, 1347, 1420, 1421, 2574, 2897
MunI CAATTG 1 cut(s) 1338
Mva1269I GAATGC 3 cut(s) 582, 1506, 2339
MvaI CCWGG 5 cut(s) 1060, 1306, 1347, 2574, 2897
MvnI CGCG 6 cut(s) 12, 33, 48, 54, 110, 771
NaeI GCCGGC 3 cut(s) 168, 219, 288
NarI GGCGCC 4 cut(s) 104, 164, 215, 284
NciI CCSGG 4 cut(s) 69, 393, 1420, 1421
NcoI CCATGG 1 cut(s) 1323
NdeI CATATG 2 cut(s) 586, 2746
NgoMIV GCCGGC 3 cut(s) 166, 217, 286
NlaIII CATG 9 cut(s) 598, 785, 1327, 1571, 1614, 1621, 2288, 2639, 2678
NmuCI GTSAC 4 cut(s) 896, 1015, 1384, 2134
NotI GCGGCCGC 1 cut(s) 49
NsbI TGCGCA 1 cut(s) 2796
PaeR7I CTCGAG 1 cut(s) 891
PagI TCATGA 2 cut(s) 1567, 2284
PceI AGGCCT 2 cut(s) 1309, 2453
PctI GAATGC 3 cut(s) 582, 1506, 2339
PdiI GCCGGC 3 cut(s) 168, 219, 288
PdmI GAANNNNTTC 1 cut(s) 2696
PfeI GAWTC 9 cut(s) 1009, 1301, 1430, 2089, 2226, 2242, 2373, 2719, 2837
PflMI CCANNNNNTGG 1 cut(s) 2150
PleI GAGTC 4 cut(s) 425, 565, 1118, 1362
PluTI GGCGCC 4 cut(s) 107, 167, 218, 287
PmaCI CACGTG 1 cut(s) 1688
PmlI CACGTG 1 cut(s) 1688
PpsI GAGTC 4 cut(s) 425, 565, 1118, 1362
Ppu21I YACGTR 1 cut(s) 1688
PpuMI RGGWCCY 2 cut(s) 887, 2899
PshBI ATTAAT 1 cut(s) 2034
Psp124BI GAGCTC 1 cut(s) 1332
Psp1406I AACGTT 1 cut(s) 1190
Psp5II RGGWCCY 2 cut(s) 887, 2899
Psp6I CCWGG 5 cut(s) 1058, 1304, 1345, 2572, 2895
PspCI CACGTG 1 cut(s) 1688
PspEI GGTNACC 1 cut(s) 896
PspFI CCCAGC 3 cut(s) 614, 2150, 2662
PspGI CCWGG 5 cut(s) 1058, 1304, 1345, 2572, 2895
PspPI GGNCC 5 cut(s) 121, 887, 1416, 1922, 2899
PspPPI RGGWCCY 2 cut(s) 887, 2899
PspXI VCTCGAGB 1 cut(s) 891
PstI CTGCAG 1 cut(s) 759
PstNI CAGNNNCTG 2 cut(s) 137, 2579
PsuI RGATCY 6 cut(s) 463, 680, 1477, 1717, 1913, 2266
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 7 cut(s) 210, 226, 551, 1046, 1250, 1779, 2578
RsaNI GTAC 7 cut(s) 209, 225, 550, 1045, 1249, 1778, 2577
RseI CAYNNNNRTG 6 cut(s) 474, 477, 806, 1572, 2673, 2919
SacI GAGCTC 1 cut(s) 1332
SacII CCGCGG 2 cut(s) 49, 55
Sau96I GGNCC 5 cut(s) 121, 887, 1416, 1922, 2899
SchI GAGTC 4 cut(s) 426, 566, 1119, 1362
ScrFI CCNGG 9 cut(s) 69, 393, 1060, 1306, 1347, 1420, 1421, 2574, 2897
SduI GDGCHC 7 cut(s) 178, 206, 262, 1332, 2759, 2818, 2916
SexAI ACCWGGT 1 cut(s) 2572
SfaNI GCATC 9 cut(s) 478, 512, 695, 1443, 1522, 2062, 2428, 2524, 2806
SfcI CTRYAG 3 cut(s) 755, 2172, 2383
SfoI GGCGCC 4 cut(s) 105, 165, 216, 285
Sfr274I CTCGAG 1 cut(s) 891
Sfr303I CCGCGG 2 cut(s) 49, 55
SgrBI CCGCGG 2 cut(s) 49, 55
SinI GGWCC 3 cut(s) 887, 1922, 2899
SlaI CTCGAG 1 cut(s) 891
SmaI CCCGGG 1 cut(s) 1421
SmiMI CAYNNNNRTG 6 cut(s) 474, 477, 806, 1572, 2673, 2919
SmlI CTYRAG 3 cut(s) 891, 1559, 2027
SmoI CTYRAG 3 cut(s) 891, 1559, 2027
SseBI AGGCCT 2 cut(s) 1309, 2453
SspDI GGCGCC 4 cut(s) 103, 163, 214, 283
SspI AATATT 2 cut(s) 976, 2566
SspMI CTAG 2 cut(s) 1536, 2801
SstI GAGCTC 1 cut(s) 1332
StuI AGGCCT 2 cut(s) 1309, 2453
StyD4I CCNGG 9 cut(s) 67, 391, 1058, 1304, 1345, 1418, 1419, 2572, 2895
StyI CCWWGG 3 cut(s) 366, 1323, 2122
TaaI ACNGT 8 cut(s) 208, 851, 1198, 1739, 1782, 2062, 2162, 2581
TaiI ACGT 3 cut(s) 1193, 1395, 1690
TaqI TCGA 7 cut(s) 200, 447, 673, 892, 1150, 1681, 2351
TatI WGTACW 3 cut(s) 549, 1248, 1777
TfiI GAWTC 9 cut(s) 1009, 1301, 1430, 2089, 2226, 2242, 2373, 2719, 2837
TseFI GTSAC 4 cut(s) 896, 1015, 1384, 2134
TseI GCWGC 8 cut(s) 757, 830, 1137, 1171, 2177, 2804, 2871, 2885
Tsp45I GTSAC 4 cut(s) 896, 1015, 1384, 2134
TspDTI ATGAA 8 cut(s) 9, 288, 611, 798, 1389, 2297, 2323, 2829
TspGWI ACGGA 2 cut(s) 345, 2461
TspMI CCCGGG 1 cut(s) 1419
Van91I CCANNNNNTGG 1 cut(s) 2150
Vha464I CTTAAG 2 cut(s) 1559, 2027
VneI GTGCAC 2 cut(s) 2755, 2912
VpaK11BI GGWCC 3 cut(s) 887, 1922, 2899
VspI ATTAAT 1 cut(s) 2034
XagI CCTNNNNNAGG 1 cut(s) 1265
XapI RAATTY 5 cut(s) 905, 1493, 1935, 2612, 2643
XcmI CCANNNNNNNNNTGG 2 cut(s) 439, 1222
XhoI CTCGAG 1 cut(s) 891
XmaI CCCGGG 1 cut(s) 1419
XmnI GAANNNNTTC 1 cut(s) 2696
XspI CTAG 2 cut(s) 1536, 2801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.