Rw1G027430

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001300
Physical Location & Seq
Reverse (-)
54908536 .. 54912268
3733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027430.1

Sequence Viewer

Length: 1056 bp
ATGCTACCACGTCATCGTGGGCATATTGATACGGTTCTCAGATATATTAACAAGCGGTCTGTCCATACACTTGAGAAGATTGGAGACAGCAAGAGACACATTCAGTTGCTGATTATCGTTTTGCTTGATGAAAGAGTATCCTATGGTGAGGAGCTAAATATGACCCAAAAGAGTGCTTTCTTGATATATATTAACAAGCGGTCTGTCCTTACACTTGAGAAGATTGGACACAGCAAGAGACACCTTCAGTTGCTGATTATCGTTTTGCTTGATGAAAGAGTATCCTATGGTTGCGTCCATTTGATCAAGCAAATAGCTCGCCGCCTCGCTAGGCCAGACAAGGTGGTTGCCTCAATGGACTGGCCGGAGGTCTATAAGTATAGAGGAGTCCTGTCACAGCAGAATCATCAACTTTTCATTGCATTTCGAGCTTCGATTGGCCAGAAGCCACGCATACATAATCATTTTCTACAGCAACACTTTCTGATATCTGGCTCTCTACTATTTGATAGGGATGGTGTCAGTGAAGGGCAGTTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACAAAAGGCCTGTGCTTCTTTGGAGGAGGGGTATATGCCTCCAGTTACTTTTGTTGTGGTACTATTAAGGCATCACACTCGCTTTTTTCTTGCTGGCAACAAGAGCCATAGGAAATGCAATGTTCTACCAGGTACCATGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTACCTTACTAGCCATGCTGCAATTCAGGTAGTTGCCCTACTCACTATCATGTCCTTGTTTGATGAGAACAAGTTCAGTGTTGATGCCTTGCAGATTCTCACCAACAATCTGTGCTACATATATGCAAGGTGCACCCGTGCAGTTTCTGTAGTGCCTCCTGTCTATTACGCTCATCTGGCTGCATCTAGAGGACGCTATTACATTAAGGGTGATGCCTCGAATGATGCTTCTCCAGGTGGCACTGCCGCACAGTTCCAGGTACTTCCTGATGTGCATCATCATGTCAAGGATGTGATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

351

Amino Acids

40.14

Weight (kDa)

9.29

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 156 - 318 8.6e-50 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 701
AciI CCGC 4 cut(s) 55, 199, 322, 993
AcoI YGGCCR 2 cut(s) 362, 439
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 3 cut(s) 630, 703, 1008
AjiI CACGTC 1 cut(s) 11
AjnI CCWGG 3 cut(s) 697, 979, 1002
AluBI AGCT 3 cut(s) 154, 317, 431
AluI AGCT 3 cut(s) 154, 317, 431
Alw21I GWGCWC 1 cut(s) 881
Alw26I GTCTC 3 cut(s) 78, 88, 232
Alw44I GTGCAC 1 cut(s) 877
AlwNI CAGNNNCTG 3 cut(s) 109, 253, 893
AoxI GGCC 4 cut(s) 332, 362, 439, 576
ApaLI GTGCAC 1 cut(s) 877
ApeKI GCWGC 2 cut(s) 764, 926
ApoI RAATTY 1 cut(s) 737
Asp700I GAANNNNTTC 1 cut(s) 818
Asp718I GGTACC 1 cut(s) 701
AsuHPI GGTGA 3 cut(s) 158, 838, 968
BaeGI GKGCMC 1 cut(s) 881
BaeI ACNNNNGTAYC 2 cut(s) 693, 726
BalI TGGCCA 1 cut(s) 441
BanI GGYRCC 1 cut(s) 701
Bbv12I GWGCWC 1 cut(s) 881
BbvI GCAGC 2 cut(s) 751, 913
BccI CCATC 3 cut(s) 509, 553, 735
BcgI CGANNNNNNTGC 4 cut(s) 299, 333, 629, 663
BciT130I CCWGG 3 cut(s) 699, 981, 1004
BciVI GTATCC 3 cut(s) 148, 292, 706
BclI TGATCA 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 78, 88, 232
BfaI CTAG 3 cut(s) 330, 756, 933
BfmI CTRYAG 2 cut(s) 470, 894
BfuI GTATCC 3 cut(s) 148, 292, 706
BisI GCNGC 4 cut(s) 322, 765, 927, 993
BlsI GCNGC 4 cut(s) 323, 766, 928, 994
Bme1390I CCNGG 3 cut(s) 699, 981, 1004
BmgBI CACGTC 1 cut(s) 11
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 3 cut(s) 699, 981, 1004
BmsI GCATC 7 cut(s) 553, 649, 820, 938, 949, 961, 1030
BpmI CTGGAG 2 cut(s) 594, 963
BpuEI CTTGAG 2 cut(s) 92, 236
BsaBI GATNNNNATC 1 cut(s) 1020
BsaJI CCNNGG 1 cut(s) 705
BsaXI ACNNNNNCTCC 2 cut(s) 584, 614
Bse1I ACTGG 2 cut(s) 365, 611
Bse3DI GCAATG 2 cut(s) 417, 694
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 3 cut(s) 699, 981, 1004
BseDI CCNNGG 1 cut(s) 705
BseGI GGATG 4 cut(s) 520, 568, 727, 1042
BseJI GATNNNNATC 1 cut(s) 1020
BseMI GCAATG 2 cut(s) 417, 694
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 2 cut(s) 365, 611
BseRI GAGGAG 3 cut(s) 164, 399, 608
BseSI GKGCMC 1 cut(s) 881
BseXI GCAGC 2 cut(s) 751, 913
BsgI GTGCAG 1 cut(s) 906
BshFI GGCC 4 cut(s) 334, 364, 441, 578
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 881
BsiSI CCGG 1 cut(s) 365
BsmAI GTCTC 3 cut(s) 78, 88, 232
BsnI GGCC 4 cut(s) 334, 364, 441, 578
Bsp1286I GDGCHC 1 cut(s) 881
Bsp143I GATC 1 cut(s) 303
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 4 cut(s) 55, 199, 322, 993
BspANI GGCC 4 cut(s) 334, 364, 441, 578
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 703
BspT107I GGYRCC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 417, 694
BsrI ACTGG 2 cut(s) 365, 611
BssECI CCNNGG 1 cut(s) 705
BssMI GATC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 3 cut(s) 699, 981, 1004
Bst4CI ACNGT 2 cut(s) 34, 999
BstC8I GCNNGC 2 cut(s) 319, 664
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 4 cut(s) 520, 568, 727, 1042
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 3 cut(s) 78, 88, 232
BstMBI GATC 1 cut(s) 303
BstMWI GCNNNNNNNGC 3 cut(s) 428, 672, 923
BstNI CCWGG 3 cut(s) 699, 981, 1004
BstSCI CCNGG 3 cut(s) 697, 979, 1002
BstSFI CTRYAG 2 cut(s) 470, 894
BstSLI GKGCMC 1 cut(s) 881
BstV1I GCAGC 2 cut(s) 751, 913
BsuI GTATCC 3 cut(s) 148, 292, 706
BsuRI GGCC 4 cut(s) 334, 364, 441, 578
BtgI CCRYGG 1 cut(s) 705
BtrI CACGTC 1 cut(s) 11
BtsCI GGATG 4 cut(s) 520, 568, 727, 1042
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 3 cut(s) 529, 829, 987
Cac8I GCNNGC 2 cut(s) 319, 664
CaiI CAGNNNCTG 3 cut(s) 109, 253, 893
CseI GACGC 2 cut(s) 283, 948
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 3 cut(s) 629, 702, 1007
CviAII CATG 4 cut(s) 706, 761, 796, 1028
CviQI GTAC 3 cut(s) 629, 702, 1007
DdeI CTNAG 1 cut(s) 38
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
EaeI YGGCCR 2 cut(s) 362, 439
Eco130I CCWWGG 1 cut(s) 705
Eco147I AGGCCT 1 cut(s) 578
Eco32I GATATC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 230
EcoRII CCWGG 3 cut(s) 697, 979, 1002
EcoRV GATATC 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 705
ErhI CCWWGG 1 cut(s) 705
FaeI CATG 4 cut(s) 709, 764, 799, 1031
FatI CATG 4 cut(s) 705, 760, 795, 1027
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 4 cut(s) 322, 765, 927, 993
FokI GGATG 4 cut(s) 527, 575, 714, 1049
Fsp4HI GCNGC 4 cut(s) 322, 765, 927, 993
FspBI CTAG 3 cut(s) 330, 756, 933
GluI GCNGC 4 cut(s) 322, 765, 927, 993
GsuI CTGGAG 2 cut(s) 594, 963
HaeIII GGCC 4 cut(s) 334, 364, 441, 578
HapII CCGG 1 cut(s) 365
HgaI GACGC 2 cut(s) 283, 948
Hin1II CATG 4 cut(s) 709, 764, 799, 1031
HinfI GANTC 3 cut(s) 387, 403, 841
HpaII CCGG 1 cut(s) 365
HphI GGTGA 3 cut(s) 158, 838, 968
Hpy166II GTNNAC 1 cut(s) 879
Hpy188I TCNGA 2 cut(s) 41, 486
Hpy188III TCNNGA 3 cut(s) 181, 933, 1013
Hpy8I GTNNAC 1 cut(s) 879
HpyAV CCTTC 2 cut(s) 254, 521
HpyCH4III ACNGT 2 cut(s) 34, 999
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 9 cut(s) 422, 687, 767, 838, 872, 879, 887, 929, 1021
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 672, 923
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 4 cut(s) 709, 764, 799, 1031
KpnI GGTACC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 2 cut(s) 751, 913
LweI GCATC 7 cut(s) 553, 649, 820, 938, 949, 961, 1030
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 3 cut(s) 330, 756, 933
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 2 cut(s) 393, 613
MalI GATC 1 cut(s) 305
MboI GATC 1 cut(s) 303
MboII GAAGA 2 cut(s) 88, 232
MhlI GDGCHC 1 cut(s) 881
MlsI TGGCCA 1 cut(s) 441
MluCI AATT 2 cut(s) 737, 768
MluNI TGGCCA 1 cut(s) 441
MlyI GAGTC 1 cut(s) 396
MmeI TCCRAC 1 cut(s) 755
Mox20I TGGCCA 1 cut(s) 441
MroXI GAANNNNTTC 1 cut(s) 818
MscI TGGCCA 1 cut(s) 441
MseI TTAA 4 cut(s) 48, 192, 635, 951
MslI CAYNNNNRTG 2 cut(s) 794, 1026
Msp20I TGGCCA 1 cut(s) 441
MspI CCGG 1 cut(s) 365
MspR9I CCNGG 3 cut(s) 699, 981, 1004
MvaI CCWGG 3 cut(s) 699, 981, 1004
MwoI GCNNNNNNNGC 3 cut(s) 428, 672, 923
NcoI CCATGG 1 cut(s) 705
NdeII GATC 1 cut(s) 303
NlaIII CATG 4 cut(s) 709, 764, 799, 1031
NlaIV GGNNCC 1 cut(s) 703
NmuCI GTSAC 1 cut(s) 393
PceI AGGCCT 1 cut(s) 578
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 2 cut(s) 403, 841
PkrI GCNGC 4 cut(s) 323, 766, 928, 994
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Psp6I CCWGG 3 cut(s) 697, 979, 1002
PspGI CCWGG 3 cut(s) 697, 979, 1002
PspN4I GGNNCC 1 cut(s) 703
PstNI CAGNNNCTG 3 cut(s) 109, 253, 893
RsaI GTAC 3 cut(s) 630, 703, 1008
RsaNI GTAC 3 cut(s) 629, 702, 1007
RseI CAYNNNNRTG 2 cut(s) 794, 1026
SaqAI TTAA 4 cut(s) 48, 192, 635, 951
SatI GCNGC 4 cut(s) 322, 765, 927, 993
Sau3AI GATC 1 cut(s) 303
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 3 cut(s) 699, 981, 1004
SduI GDGCHC 1 cut(s) 881
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 7 cut(s) 553, 649, 820, 938, 949, 961, 1030
SfcI CTRYAG 2 cut(s) 470, 894
SmiMI CAYNNNNRTG 2 cut(s) 794, 1026
SmlI CTYRAG 2 cut(s) 71, 215
SmoI CTYRAG 2 cut(s) 71, 215
Sse9I AATT 2 cut(s) 737, 768
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 4 cut(s) 55, 199, 322, 993
SspMI CTAG 3 cut(s) 330, 756, 933
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 3 cut(s) 697, 979, 1002
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 2 cut(s) 34, 999
TaiI ACGT 1 cut(s) 13
TaqI TCGA 3 cut(s) 427, 434, 965
TasI AATT 2 cut(s) 737, 768
TauI GCSGC 2 cut(s) 324, 995
TfiI GAWTC 2 cut(s) 403, 841
Tru1I TTAA 4 cut(s) 48, 192, 635, 951
Tru9I TTAA 4 cut(s) 48, 192, 635, 951
TscAI CASTG 3 cut(s) 529, 829, 994
TseFI GTSAC 1 cut(s) 393
TseI GCWGC 2 cut(s) 764, 926
Tsp45I GTSAC 1 cut(s) 393
TspDTI ATGAA 3 cut(s) 144, 288, 406
TspRI CASTG 3 cut(s) 529, 829, 994
VneI GTGCAC 1 cut(s) 877
XapI RAATTY 1 cut(s) 737
XbaI TCTAGA 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 3 cut(s) 330, 756, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.