Rh5AG368300

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001300
Physical Location & Seq
Reverse (-)
62168491 .. 62174821
6331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG368300.1

Sequence Viewer

Length: 2778 bp
ATGTCCAATCGCGGCGGTTCATCCGGTAGTCGCGGCGGCGGAGGCCGCGGCCGCGGCCGCGGAAGAGGAAATCCCGGCGGTCAGGCCAGAGGCTCAAGCTACACTCCCGCGCCCGCGCCTGCTCCGGCGTATGCACCAGCTCCTGCTCCGGCGCCGGCTCGTGCTCAGCCGCCTGCTGTGGCCTCGTCGAGCACCGTTCCGGCGCCGGCTCGTACTCATCAGCCTGCTGTGGCCTCGTCGAGCAGTGCTCCGGCTCCGGCTCCGGCTCCGGCGCCAGCTCCGGCGCCGGCTCCGGCGCCAGCTCCGGCGCCGGCTCCGGCACCTGTTTCATCGGCCGCGGCGGCTTCGCTGGCGAGTGAGATGGAAGAGAAACTTGCATTGGCTCCGTCGTCATCCAAGGCGGTGAGGTTTCCAGTGAGGCCGGGGTTTGGTAGAGCTGGTCAAAGAGTCCAAGTCCGAGCCAATCATTTTCTGGTCGAGATTGGGGAAAGAGATCTGCATCACTATGATGTATCAATTAGCCCTGAAGTTGCATCAAAGAAGATAAACAGAGATGTAATCAACCAGCTGGTTCAGATGTACTCTGAGTCTCACTTGGGAAAGAGAATGCCAGCGTATGATGGCATGAAGAGTATCTACACTGCCGGGCCATTGCCCTTCTCTTCAAAGGAGTTTGTGGTCAAGCTACCTGAGAGAGAAGGTCGAGCTGCATCTGCATCAAGAAGGAAAGATAGAGACTTCAAGGTCTGCATTAAGTTGGCAAACAAGCCTGACCTGTATCAACTGCAGCAGTTCTTACGCGGAAGACTACACGAAACACCACAAGAGACTATCCAAGTTCTTGATGTTGTTCTTAGAGCTGCACCATCACAGAAATACTGTGTGGTTGGTAGGTCATTTTTTAGCCCTGATCTGGGTCCTCGAGGTGACCTTGAATTTGGCTTGGAATACTGGAGAGGCTTTTACCAAAGCCTAAGGCCAACTCAGTTTGGTCTATCCCTAAATATTGATATCTCAGCAAGGGCTTTTTATGATCCGATTCTGGTGACAGAGTTTATTAAGAAACATTTTGGGTACAGAGATTTATCCAGGGGTCTGTCGGATCGTGATTGTCTGAAGCTGAAGAAAAATCTGAAAGGAATCAAGGTAACTATGAGCTGTGGAGGCAGCAGAAGTTATCGAATTACTGGAGTATCTGTGCAGCCACTAAGCCAGTTATCGTTCACTGTTGAGGATAATCAAACCAGAGTATCAGTGGTTCAGTATTATCGTGAGAAGTACAACATCAGCCTAAAACAGGTGGCTTTGCCTGCCCTTCAATCTGGCAATGATTCCAGGCCTGCTTATTTGCCCATGGAGCTCGCTACAATTGTTCCTGGGCAGAGATACACGAAAAGACTCAATGAAAAGCAAGTGACAAACGTGCTGAGAGCAACTTGCCAACGGCCCCGGGAGAGGGAATCCAATATACTGAAGATGGTGATGCACAATAATTACAAGGGAGAGGATCTTGTAAACAAAGAATTTGGAATGCAAGTGAAAGAACAAATGGCACTGGTGGATGCTAGAGTATTACCACCTCCAATGCTTAAGTATCATGAGAGTGGTCGTGAAACAAAGGATACTCCGAGGATGGGTGCATGGAACATGATCAATAAGAAAATGGTTAATGGTGCCAAAGTGGATTTCTGGGCTTTTGTCAATTTCTCTCGATCACGTGAAGATTTTAACTATCGCTTTTGTGAGGATCTCGTCTATATGTGCAACAGTAAAGGGGTGGAGTTCCATTCCGAGCCTTTACTTCCTGTACAGTCAGCTCATCCTAATCAAATTGAGAGGGTTCTGAGAGATATTCACAAGCAGTCAGTCCAGAGACTTAATGAGATGGGACAAGCCAGCAAACATCTTCAGTTGTTGATTATCATCCTGCCTGATGTCAAAGGATCTTATGGTCCCATTAAAAGAATTTGTGAAACAGAGCTTGGAATTGTCTCCCAGTGCTGTAATCCTAAGCAAGCACAAAAGCTTAGTAAGCAATACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGGTTGGTGGTAGAAACACAGTCTTAAATGACGCCATTCAGAGGAGGATTCCTCTCGTCAGTGATCGTCCTACAATCATCCTTGGAGCTGATGTCACTCATCCACAAGCTGGGGAAGACAGTAGCCCATCTATAGCAGCAGTGGTTGCCTCAATGGACTGGCCAGAGGTCTCCAAGTATAGAGGAATCCTGTCAGCACAGAATCATCGTCAAGAAATCATCCAAGATCTTTACAAATTAACTCACGATGAGAAAAGAGGGTCGGTTCATTCAGGAATGATCAGAGAACTTTTCATTGCATTCCGAAGATCGACTGGCCAAAAGCCGCACAGAATCATCTTCTATAGGGATGGGGTGAGTGAAGGGCAGTTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACGGAAGGCCTGTGCTTCTTTGGAGGAGGGGTATATGCCACCAGTTACTTTTGTTGTGGTGCAGAAAAGGCATCACACACGCCTTTTCCCTGCTGACAACCAGACAGATAGGAGTGGGAATATTCAACCAGGTACTGTGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTATCTTAATAGCCATGCAGGAATTCAGGGAACAAGTCGCCCAGCTCATTATCATGTCCTGTTTGATGAGAACAACTTCACTGCTGATGGCTTGCAGATTCTCACCAACAATCTGTGCTACACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

925

Amino Acids

102.59

Weight (kDa)

9.6

Isoelectric Point (pI)

54.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 148 - 285 1.8e-37 N-terminal domain of argonaute
ArgoL1 PF08699 296 - 344 3.2e-18 Argonaute linker 1 domain
PAZ PF02170 372 - 475 2.9e-25 PAZ domain
ArgoL2 PF16488 485 - 532 2.2e-13 Argonaute linker 2 domain
ArgoMid PF16487 542 - 620 4.5e-09 Mid domain of argonaute
Piwi PF02171 639 - 925 2.3e-94 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 743
AbsI CCTCGAGG 1 cut(s) 921
AccB1I GGYRCC 8 cut(s) 151, 202, 271, 283, 295, 307, 319, 1673
AccB7I CCANNNNNTGG 1 cut(s) 2180
AccII CGCG 9 cut(s) 12, 33, 48, 54, 60, 110, 116, 338, 801
AclWI GGATC 5 cut(s) 1028, 1110, 1515, 1755, 1951
AcoI YGGCCR 5 cut(s) 49, 55, 333, 2231, 2386
AcsI RAATTY 5 cut(s) 935, 1523, 1965, 2642, 2673
AcuI CTGAAG 5 cut(s) 546, 1136, 1142, 1493, 1892
AcvI CACGTG 1 cut(s) 1718
AcyI GRCGYC 7 cut(s) 152, 203, 272, 284, 296, 308, 2103
AfaI GTAC 6 cut(s) 214, 581, 1076, 1280, 1809, 2608
AfiI CCNNNNNNNGG 9 cut(s) 428, 913, 914, 1297, 1455, 1456, 1634, 2112, 2180
AflII CTTAAG 2 cut(s) 1589, 2057
AflIII ACRYGT 1 cut(s) 2772
AgsI TTSAA 6 cut(s) 666, 742, 935, 1319, 2045, 2600
AjnI CCWGG 4 cut(s) 1088, 1334, 1375, 2602
AjuI GAANNNNNNNTTGG 6 cut(s) 362, 394, 1670, 1702, 1965, 1997
Alw21I GWGCWC 4 cut(s) 166, 194, 250, 1362
Alw26I GTCTC 7 cut(s) 594, 729, 821, 1867, 1996, 2058, 2245
AlwI GGATC 5 cut(s) 1028, 1110, 1515, 1755, 1951
AlwNI CAGNNNCTG 2 cut(s) 143, 2609
Ama87I CYCGRG 2 cut(s) 921, 1449
ApeKI GCWGC 6 cut(s) 707, 787, 860, 1167, 1201, 2207
ApoI RAATTY 5 cut(s) 935, 1523, 1965, 2642, 2673
ArsI GACNNNNNNTTYG 2 cut(s) 920, 952
AseI ATTAAT 1 cut(s) 2064
Asp700I GAANNNNTTC 1 cut(s) 2726
AspLEI GCGC 8 cut(s) 112, 118, 154, 205, 274, 286, 298, 310
AspS9I GGNCC 4 cut(s) 647, 917, 1446, 1952
AsuC2I CCSGG 5 cut(s) 75, 423, 646, 1450, 1451
AsuHPI GGTGA 6 cut(s) 415, 938, 1057, 1492, 2437, 2746
AvaI CYCGRG 2 cut(s) 921, 1449
AvaII GGWCC 2 cut(s) 917, 1952
AxyI CCTNAGG 1 cut(s) 974
BaeI ACNNNNGTAYC 6 cut(s) 1176, 1209, 1233, 1266, 2598, 2631
BalI TGGCCA 2 cut(s) 2233, 2388
BanI GGYRCC 8 cut(s) 151, 202, 271, 283, 295, 307, 319, 1673
BanII GRGCYC 1 cut(s) 1362
BarI GAAGNNNNNNTAC 2 cut(s) 620, 652
BauI CACGAG 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 1718
BbsI GAAGAC 2 cut(s) 811, 2193
Bbv12I GWGCWC 4 cut(s) 166, 194, 250, 1362
BbvI GCAGC 6 cut(s) 694, 799, 847, 1179, 1213, 2219
BceAI ACGGC 1 cut(s) 1460
BciT130I CCWGG 4 cut(s) 1090, 1336, 1377, 2604
BciVI GTATCC 2 cut(s) 1615, 2611
BclI TGATCA 2 cut(s) 1650, 2349
BcnI CCSGG 5 cut(s) 75, 423, 646, 1450, 1451
BcoDI GTCTC 7 cut(s) 594, 729, 821, 1867, 1996, 2058, 2245
BfaI CTAG 1 cut(s) 1566
BfmI CTRYAG 3 cut(s) 785, 2202, 2413
BfoI RGCGCY 6 cut(s) 155, 206, 275, 287, 299, 311
BfrI CTTAAG 2 cut(s) 1589, 2057
BfuI GTATCC 2 cut(s) 1615, 2611
BglI GCCNNNNNGGC 1 cut(s) 341
BglII AGATCT 2 cut(s) 493, 2296
BlpI GCTNAGC 1 cut(s) 165
Bme1390I CCNGG 9 cut(s) 75, 423, 646, 1090, 1336, 1377, 1450, 1451, 2604
Bme18I GGWCC 2 cut(s) 917, 1952
BmeT110I CYCGRG 2 cut(s) 921, 1449
BmgT120I GGNCC 4 cut(s) 647, 917, 1446, 1952
BmrFI CCNGG 9 cut(s) 75, 423, 646, 1090, 1336, 1377, 1450, 1451, 2604
BmrI ACTGGG 1 cut(s) 1990
BmsI GCATC 8 cut(s) 508, 542, 719, 725, 1473, 1552, 2458, 2554
BmuI ACTGGG 1 cut(s) 1990
BpiI GAAGAC 2 cut(s) 811, 2193
BplI GAGNNNNNCTC 4 cut(s) 232, 264, 2107, 2139
BpmI CTGGAG 2 cut(s) 973, 1209
Bpu10I CCTNAGC 1 cut(s) 2010
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 5 cut(s) 75, 423, 646, 1450, 1451
BsaAI YACGTR 2 cut(s) 1718, 2775
BsaBI GATNNNNATC 3 cut(s) 498, 1922, 2652
BsaHI GRCGYC 7 cut(s) 152, 203, 272, 284, 296, 308, 2103
BsaI GGTCTC 1 cut(s) 2245
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 6 cut(s) 1182, 1212, 2148, 2178, 2489, 2519
Bsc4I CCNNNNNNNGG 9 cut(s) 428, 913, 914, 1297, 1455, 1456, 1634, 2112, 2180
Bse118I RCCGGY 4 cut(s) 154, 205, 286, 310
Bse1I ACTGG 9 cut(s) 413, 956, 1192, 1213, 1560, 1996, 2234, 2389, 2516
Bse21I CCTNAGG 1 cut(s) 974
Bse3DI GCAATG 3 cut(s) 650, 1333, 2364
Bse8I GATNNNNATC 3 cut(s) 498, 1922, 2652
BseBI CCWGG 4 cut(s) 1090, 1336, 1377, 2604
BseJI GATNNNNATC 3 cut(s) 498, 1922, 2652
BseLI CCNNNNNNNGG 9 cut(s) 428, 913, 914, 1297, 1455, 1456, 1634, 2112, 2180
BseMI GCAATG 3 cut(s) 650, 1333, 2364
BseMII CTCAG 7 cut(s) 179, 576, 681, 998, 1029, 1418, 1835
BseNI ACTGG 9 cut(s) 413, 956, 1192, 1213, 1560, 1996, 2234, 2389, 2516
BseRI GAGGAG 2 cut(s) 2128, 2513
BseX3I CGGCCG 3 cut(s) 49, 55, 333
BseXI GCAGC 6 cut(s) 694, 799, 847, 1179, 1213, 2219
BseYI CCCAGC 2 cut(s) 2180, 2692
BsgI GTGCAG 3 cut(s) 846, 1220, 2555
Bsh1236I CGCG 9 cut(s) 12, 33, 48, 54, 60, 110, 116, 338, 801
Bsh1285I CGRYCG 3 cut(s) 52, 58, 336
BshNI GGYRCC 8 cut(s) 151, 202, 271, 283, 295, 307, 319, 1673
BsiEI CGRYCG 3 cut(s) 52, 58, 336
BsiHKAI GWGCWC 4 cut(s) 166, 194, 250, 1362
BsiHKCI CYCGRG 2 cut(s) 921, 1449
BslFI GGGAC 2 cut(s) 1902, 1938
BslI CCNNNNNNNGG 9 cut(s) 428, 913, 914, 1297, 1455, 1456, 1634, 2112, 2180
BsmAI GTCTC 7 cut(s) 594, 729, 821, 1867, 1996, 2058, 2245
BsmFI GGGAC 2 cut(s) 1902, 1938
BsmI GAATGC 3 cut(s) 612, 1536, 2369
Bso31I GGTCTC 1 cut(s) 2245
BsoBI CYCGRG 2 cut(s) 921, 1449
Bsp1286I GDGCHC 4 cut(s) 166, 194, 250, 1362
Bsp1407I TGTACA 1 cut(s) 1807
Bsp1720I GCTNAGC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 1353
BspCNI CTCAG 7 cut(s) 178, 577, 682, 997, 1028, 1419, 1836
BspFNI CGCG 9 cut(s) 12, 33, 48, 54, 60, 110, 116, 338, 801
BspHI TCATGA 1 cut(s) 1597
BspMAI CTGCAG 1 cut(s) 789
BspPI GGATC 5 cut(s) 1028, 1110, 1515, 1755, 1951
BspT107I GGYRCC 8 cut(s) 151, 202, 271, 283, 295, 307, 319, 1673
BspTI CTTAAG 2 cut(s) 1589, 2057
BspTNI GGTCTC 1 cut(s) 2245
BsrDI GCAATG 3 cut(s) 650, 1333, 2364
BsrFI RCCGGY 4 cut(s) 154, 205, 286, 310
BsrGI TGTACA 1 cut(s) 1807
BsrI ACTGG 9 cut(s) 413, 956, 1192, 1213, 1560, 1996, 2234, 2389, 2516
BssAI RCCGGY 4 cut(s) 154, 205, 286, 310
BssNI GRCGYC 7 cut(s) 152, 203, 272, 284, 296, 308, 2103
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 3 cut(s) 396, 1353, 2152
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 4 cut(s) 1090, 1336, 1377, 2604
Bst4CI ACNGT 8 cut(s) 196, 881, 1228, 1769, 1812, 2092, 2192, 2611
Bst6I CTCTTC 4 cut(s) 58, 360, 623, 667
BstACI GRCGYC 7 cut(s) 152, 203, 272, 284, 296, 308, 2103
BstAFI CTTAAG 2 cut(s) 1589, 2057
BstAPI GCANNNNNTGC 1 cut(s) 2216
BstAUI TGTACA 1 cut(s) 1807
BstBAI YACGTR 2 cut(s) 1718, 2775
BstDSI CCRYGG 5 cut(s) 46, 52, 58, 336, 1353
BstEII GGTNACC 1 cut(s) 926
BstENI CCTNNNNNAGG 1 cut(s) 1295
BstFNI CGCG 9 cut(s) 12, 33, 48, 54, 60, 110, 116, 338, 801
BstH2I RGCGCY 6 cut(s) 155, 206, 275, 287, 299, 311
BstHHI GCGC 8 cut(s) 112, 118, 154, 205, 274, 286, 298, 310
BstMAI GTCTC 7 cut(s) 594, 729, 821, 1867, 1996, 2058, 2245
BstMCI CGRYCG 3 cut(s) 52, 58, 336
BstNI CCWGG 4 cut(s) 1090, 1336, 1377, 2604
BstPI GGTNACC 1 cut(s) 926
BstSCI CCNGG 9 cut(s) 73, 421, 644, 1088, 1334, 1375, 1448, 1449, 2602
BstSFI CTRYAG 3 cut(s) 785, 2202, 2413
BstUI CGCG 9 cut(s) 12, 33, 48, 54, 60, 110, 116, 338, 801
BstV1I GCAGC 6 cut(s) 694, 799, 847, 1179, 1213, 2219
BstV2I GAAGAC 2 cut(s) 811, 2193
BstX2I RGATCY 5 cut(s) 493, 1507, 1747, 1943, 2296
BstYI RGATCY 5 cut(s) 493, 1507, 1747, 1943, 2296
BstZI CGGCCG 3 cut(s) 49, 55, 333
Bsu36I CCTNAGG 1 cut(s) 974
BsuI GTATCC 2 cut(s) 1615, 2611
BtgI CCRYGG 5 cut(s) 46, 52, 58, 336, 1353
BtsI GCAGTG 4 cut(s) 250, 639, 2217, 2730
CaiI CAGNNNCTG 2 cut(s) 143, 2609
CciI TCATGA 1 cut(s) 1597
CciNI GCGGCCGC 2 cut(s) 49, 55
CfoI GCGC 8 cut(s) 112, 118, 154, 205, 274, 286, 298, 310
Cfr10I RCCGGY 4 cut(s) 154, 205, 286, 310
Cfr13I GGNCC 4 cut(s) 647, 917, 1446, 1952
Cfr42I CCGCGG 4 cut(s) 49, 55, 61, 339
Cfr9I CCCGGG 1 cut(s) 1449
CseI GACGC 1 cut(s) 2111
CsiI ACCWGGT 1 cut(s) 2602
Csp6I GTAC 6 cut(s) 213, 580, 1075, 1279, 1808, 2607
CspCI CAANNNNNGTGG 2 cut(s) 2059, 2094
CviAII CATG 7 cut(s) 625, 1354, 1598, 1641, 1648, 2666, 2705
CviQI GTAC 6 cut(s) 213, 580, 1075, 1279, 1808, 2607
DinI GGCGCC 6 cut(s) 153, 204, 273, 285, 297, 309
DrdI GACNNNNNNGTC 1 cut(s) 743
DseDI GACNNNNNNGTC 1 cut(s) 743
EaeI YGGCCR 5 cut(s) 49, 55, 333, 2231, 2386
EagI CGGCCG 3 cut(s) 49, 55, 333
Eam1104I CTCTTC 4 cut(s) 58, 360, 623, 667
EarI CTCTTC 4 cut(s) 58, 360, 623, 667
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 1360
EclXI CGGCCG 3 cut(s) 49, 55, 333
Eco130I CCWWGG 3 cut(s) 396, 1353, 2152
Eco147I AGGCCT 2 cut(s) 1339, 2483
Eco24I GRGCYC 1 cut(s) 1362
Eco31I GGTCTC 1 cut(s) 2245
Eco32I GATATC 1 cut(s) 1012
Eco47I GGWCC 2 cut(s) 917, 1952
Eco52I CGGCCG 3 cut(s) 49, 55, 333
Eco53kI GAGCTC 1 cut(s) 1360
Eco57I CTGAAG 5 cut(s) 546, 1136, 1142, 1493, 1892
Eco72I CACGTG 1 cut(s) 1718
Eco81I CCTNAGG 1 cut(s) 974
Eco88I CYCGRG 2 cut(s) 921, 1449
Eco91I GGTNACC 1 cut(s) 926
EcoICRI GAGCTC 1 cut(s) 1360
EcoNI CCTNNNNNAGG 1 cut(s) 1295
EcoO109I RGGNCCY 1 cut(s) 917
EcoO65I GGTNACC 1 cut(s) 926
EcoRI GAATTC 1 cut(s) 2673
EcoRII CCWGG 4 cut(s) 1088, 1334, 1375, 2602
EcoRV GATATC 1 cut(s) 1012
EcoT14I CCWWGG 3 cut(s) 396, 1353, 2152
EcoT38I GRGCYC 1 cut(s) 1362
EgeI GGCGCC 6 cut(s) 153, 204, 273, 285, 297, 309
EheI GGCGCC 6 cut(s) 153, 204, 273, 285, 297, 309
ErhI CCWWGG 3 cut(s) 396, 1353, 2152
FaeI CATG 7 cut(s) 628, 1357, 1601, 1644, 1651, 2669, 2708
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 1902, 1938
FatI CATG 7 cut(s) 624, 1353, 1597, 1640, 1647, 2665, 2704
FauI CCCGC 2 cut(s) 115, 121
FbaI TGATCA 2 cut(s) 1650, 2349
FriOI GRGCYC 1 cut(s) 1362
FspBI CTAG 1 cut(s) 1566
GlaI GCGC 8 cut(s) 111, 117, 153, 204, 273, 285, 297, 309
GsaI CCCAGC 2 cut(s) 2184, 2696
GsuI CTGGAG 2 cut(s) 973, 1209
HaeII RGCGCY 6 cut(s) 155, 206, 275, 287, 299, 311
HgaI GACGC 1 cut(s) 2111
HhaI GCGC 8 cut(s) 112, 118, 154, 205, 274, 286, 298, 310
Hin1I GRCGYC 7 cut(s) 152, 203, 272, 284, 296, 308, 2103
Hin1II CATG 7 cut(s) 628, 1357, 1601, 1644, 1651, 2669, 2708
Hin6I GCGC 8 cut(s) 110, 116, 152, 203, 272, 284, 296, 308
HinP1I GCGC 8 cut(s) 110, 116, 152, 203, 272, 284, 296, 308
HindIII AAGCTT 1 cut(s) 2024
HphI GGTGA 6 cut(s) 415, 938, 1057, 1492, 2437, 2746
Hpy166II GTNNAC 2 cut(s) 1224, 1516
Hpy8I GTNNAC 2 cut(s) 1224, 1516
Hpy99I CGWCG 3 cut(s) 190, 241, 391
HpyAV CCTTC 6 cut(s) 667, 692, 717, 1325, 2426, 2473
HpyCH4III ACNGT 8 cut(s) 196, 881, 1228, 1769, 1812, 2092, 2192, 2611
HpyCH4IV ACGT 3 cut(s) 1422, 1717, 2774
HpySE526I ACGT 3 cut(s) 1422, 1717, 2774
Hsp92I GRCGYC 7 cut(s) 152, 203, 272, 284, 296, 308, 2103
Hsp92II CATG 7 cut(s) 628, 1357, 1601, 1644, 1651, 2669, 2708
HspAI GCGC 8 cut(s) 110, 116, 152, 203, 272, 284, 296, 308
KasI GGCGCC 6 cut(s) 151, 202, 271, 283, 295, 307
KroI GCCGGC 4 cut(s) 154, 205, 286, 310
KroNI GCCGGC 4 cut(s) 156, 207, 288, 312
Ksp22I TGATCA 2 cut(s) 1650, 2349
KspI CCGCGG 4 cut(s) 49, 55, 61, 339
Lsp1109I GCAGC 6 cut(s) 694, 799, 847, 1179, 1213, 2219
LweI GCATC 8 cut(s) 508, 542, 719, 725, 1473, 1552, 2458, 2554
MabI ACCWGGT 1 cut(s) 2602
MaeI CTAG 1 cut(s) 1566
MaeII ACGT 3 cut(s) 1422, 1717, 2774
MaeIII GTNAC 6 cut(s) 926, 1045, 1147, 1414, 2164, 2518
MfeI CAATTG 1 cut(s) 1368
MflI RGATCY 5 cut(s) 493, 1507, 1747, 1943, 2296
MhlI GDGCHC 4 cut(s) 166, 194, 250, 1362
MlsI TGGCCA 2 cut(s) 2233, 2388
MluNI TGGCCA 2 cut(s) 2233, 2388
Mly113I GGCGCC 6 cut(s) 152, 203, 272, 284, 296, 308
MlyI GAGTC 3 cut(s) 456, 596, 1392
MmeI TCCRAC 2 cut(s) 1080, 2660
Mox20I TGGCCA 2 cut(s) 2233, 2388
MroNI GCCGGC 4 cut(s) 154, 205, 286, 310
MroXI GAANNNNTTC 1 cut(s) 2726
MscI TGGCCA 2 cut(s) 2233, 2388
MslI CAYNNNNRTG 4 cut(s) 504, 507, 1602, 2703
Msp20I TGGCCA 2 cut(s) 2233, 2388
MspA1I CMGCKG 5 cut(s) 48, 54, 60, 338, 568
MspCI CTTAAG 2 cut(s) 1589, 2057
MspR9I CCNGG 9 cut(s) 75, 423, 646, 1090, 1336, 1377, 1450, 1451, 2604
MunI CAATTG 1 cut(s) 1368
Mva1269I GAATGC 3 cut(s) 612, 1536, 2369
MvaI CCWGG 4 cut(s) 1090, 1336, 1377, 2604
MvnI CGCG 9 cut(s) 12, 33, 48, 54, 60, 110, 116, 338, 801
NaeI GCCGGC 4 cut(s) 156, 207, 288, 312
NarI GGCGCC 6 cut(s) 152, 203, 272, 284, 296, 308
NciI CCSGG 5 cut(s) 75, 423, 646, 1450, 1451
NcoI CCATGG 1 cut(s) 1353
NgoMIV GCCGGC 4 cut(s) 154, 205, 286, 310
NlaIII CATG 7 cut(s) 628, 1357, 1601, 1644, 1651, 2669, 2708
NmuCI GTSAC 4 cut(s) 926, 1045, 1414, 2164
NotI GCGGCCGC 2 cut(s) 49, 55
PaeR7I CTCGAG 1 cut(s) 921
PagI TCATGA 1 cut(s) 1597
PceI AGGCCT 2 cut(s) 1339, 2483
PctI GAATGC 3 cut(s) 612, 1536, 2369
PdiI GCCGGC 4 cut(s) 156, 207, 288, 312
PdmI GAANNNNTTC 1 cut(s) 2726
PfeI GAWTC 9 cut(s) 1039, 1140, 1331, 1460, 2119, 2256, 2272, 2403, 2749
PflMI CCANNNNNTGG 1 cut(s) 2180
PleI GAGTC 3 cut(s) 455, 595, 1392
PluTI GGCGCC 6 cut(s) 155, 206, 275, 287, 299, 311
PmaCI CACGTG 1 cut(s) 1718
PmlI CACGTG 1 cut(s) 1718
PpsI GAGTC 3 cut(s) 455, 595, 1392
Ppu21I YACGTR 2 cut(s) 1718, 2775
PpuMI RGGWCCY 1 cut(s) 917
PshBI ATTAAT 1 cut(s) 2064
Psp124BI GAGCTC 1 cut(s) 1362
Psp5II RGGWCCY 1 cut(s) 917
Psp6I CCWGG 4 cut(s) 1088, 1334, 1375, 2602
PspCI CACGTG 1 cut(s) 1718
PspEI GGTNACC 1 cut(s) 926
PspFI CCCAGC 2 cut(s) 2180, 2692
PspGI CCWGG 4 cut(s) 1088, 1334, 1375, 2602
PspPI GGNCC 4 cut(s) 647, 917, 1446, 1952
PspPPI RGGWCCY 1 cut(s) 917
PspXI VCTCGAGB 1 cut(s) 921
PsrI GAACNNNNNNTAC 2 cut(s) 1242, 1274
PstI CTGCAG 1 cut(s) 789
PstNI CAGNNNCTG 2 cut(s) 143, 2609
PsuI RGATCY 5 cut(s) 493, 1507, 1747, 1943, 2296
PvuII CAGCTG 1 cut(s) 568
RsaI GTAC 6 cut(s) 214, 581, 1076, 1280, 1809, 2608
RsaNI GTAC 6 cut(s) 213, 580, 1075, 1279, 1808, 2607
RseI CAYNNNNRTG 4 cut(s) 504, 507, 1602, 2703
SacI GAGCTC 1 cut(s) 1362
SacII CCGCGG 4 cut(s) 49, 55, 61, 339
Sau96I GGNCC 4 cut(s) 647, 917, 1446, 1952
SchI GAGTC 3 cut(s) 456, 596, 1392
ScrFI CCNGG 9 cut(s) 75, 423, 646, 1090, 1336, 1377, 1450, 1451, 2604
SduI GDGCHC 4 cut(s) 166, 194, 250, 1362
SexAI ACCWGGT 1 cut(s) 2602
SfaNI GCATC 8 cut(s) 508, 542, 719, 725, 1473, 1552, 2458, 2554
SfcI CTRYAG 3 cut(s) 785, 2202, 2413
SfoI GGCGCC 6 cut(s) 153, 204, 273, 285, 297, 309
Sfr274I CTCGAG 1 cut(s) 921
Sfr303I CCGCGG 4 cut(s) 49, 55, 61, 339
SgrBI CCGCGG 4 cut(s) 49, 55, 61, 339
SinI GGWCC 2 cut(s) 917, 1952
SlaI CTCGAG 1 cut(s) 921
SmaI CCCGGG 1 cut(s) 1451
SmiMI CAYNNNNRTG 4 cut(s) 504, 507, 1602, 2703
SmlI CTYRAG 4 cut(s) 94, 921, 1589, 2057
SmoI CTYRAG 4 cut(s) 94, 921, 1589, 2057
SseBI AGGCCT 2 cut(s) 1339, 2483
SspDI GGCGCC 6 cut(s) 151, 202, 271, 283, 295, 307
SspI AATATT 2 cut(s) 1006, 2596
SspMI CTAG 1 cut(s) 1566
SstI GAGCTC 1 cut(s) 1362
StuI AGGCCT 2 cut(s) 1339, 2483
StyD4I CCNGG 9 cut(s) 73, 421, 644, 1088, 1334, 1375, 1448, 1449, 2602
StyI CCWWGG 3 cut(s) 396, 1353, 2152
TaaI ACNGT 8 cut(s) 196, 881, 1228, 1769, 1812, 2092, 2192, 2611
TaiI ACGT 3 cut(s) 1425, 1720, 2777
TaqI TCGA 8 cut(s) 188, 239, 477, 703, 922, 1180, 1711, 2381
TatI WGTACW 3 cut(s) 579, 1278, 1807
TfiI GAWTC 9 cut(s) 1039, 1140, 1331, 1460, 2119, 2256, 2272, 2403, 2749
TseFI GTSAC 4 cut(s) 926, 1045, 1414, 2164
TseI GCWGC 6 cut(s) 707, 787, 860, 1167, 1201, 2207
Tsp45I GTSAC 4 cut(s) 926, 1045, 1414, 2164
TspDTI ATGAA 6 cut(s) 9, 318, 641, 1419, 2327, 2353
TspGWI ACGGA 2 cut(s) 375, 2491
TspMI CCCGGG 1 cut(s) 1449
Van91I CCANNNNNTGG 1 cut(s) 2180
Vha464I CTTAAG 2 cut(s) 1589, 2057
VpaK11BI GGWCC 2 cut(s) 917, 1952
VspI ATTAAT 1 cut(s) 2064
XagI CCTNNNNNAGG 1 cut(s) 1295
XapI RAATTY 5 cut(s) 935, 1523, 1965, 2642, 2673
XcmI CCANNNNNNNNNTGG 2 cut(s) 469, 1252
XhoI CTCGAG 1 cut(s) 921
XmaI CCCGGG 1 cut(s) 1449
XmnI GAANNNNTTC 1 cut(s) 2726
XspI CTAG 1 cut(s) 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.