Rw5G034580

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001300
Physical Location & Seq
Reverse (-)
59547570 .. 59570634
23065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034580.1

Sequence Viewer

Length: 2982 bp
ATGCGAGCAGGGACAACAGCCCATGGAGGGAGGGAGGGAGGGAGGTTTGCCAACACCGCAATGTCCAATCGCGGAGGTTCATCCGGTAGTCGCGGCGGCGGAGGCCGCGGCCGCGGCCGCGGAAGAGGAAATCCCGGCGGTCAGGCCAGAGGCTCAGGCTACACTCCGGCGCCCGCGCCTGCTCCGGCGCCTGCTCCAGCTCCAGCTCCTGCTCCGGCGTATGCTCCGGCGCCGGCTCGTGCTCAGCCGCCTGCTGTGGTCTCGTCGAGCAGGGCTCCGGCTCCGGCTCCAGCTCCTGCTCCGGCTCCGGCGCCGGCACCTGTTTCATCGGCGGCGGCGGCGGCGGCTTCGCTGGCGAGTGAGATGGAAGAGAAGCTTGCATTGGCTCCGTCGTCATCCAAGGCCGTGAGGTTTCCAGTGAGGCCGGGGTTTGGTAGAGCTGGTAAAAGAGTCCAAGTCCGAGCCAATCATTTTCTGGTCGAGATTGGGGAAAGAGATCTGCATCACTATGATGTATCAATTAGCCCTGAAGTTGCTTCAAAGAAGATAAATAGAGATGTAATCAACCAGCTGGTTCAGATGTACTCTGAGTCTCACTTGGGAAAGAGAATGCCAGCATATGATGGCATGAAGAGTATCTACACTGCCGGGCCATTGCCCTTCTCTTCAAAGGAGTTTGTGGTCAAGCTACCTGAGAGAGAAGGTCGAGCTGGATCTGCATCAAGAAGGAAAGATAGAGACTTCAAGGTCTGCATTAAGTTGGCAAACAAGCCTGACCTGTATCAACTGCAGCAGTTCTTACGCGGAGGACTACACGAAACACCACAGGAGACCATCCAAGTTCTTGATGTTGTTCTTAGAGCTGCACCATCACAGAAATACTGTGTGGTTGGTAGGTCATTTTTTAGCCCTGATCTGGGTCCTCGAGGTGACCTTGAATTTGGCTTGGAATACTGGAGAGGCTTTTACCAAAGCCTAAGGCCAACTCAGTTTGGTCTATCCCTAAATATTGATATATCAGCAAGGGCTTTTTATGATCCGATTCTGGTGACAGAGTTTATTAAGAAACATTTTGGGTACAGAGATTTATCCAGGGGTCTGTCGGATCGTGATTGTCTGAAGCTGAAGAAAAATCTGAAAGGAATCAAGGTAACTCTGAGCTATGGAGGTAGCAGAAGTTATCGAATTACTGGAGTATCTGCGCAGCCACTAAGCCAGTTAACGTTTACTCTTGAGGATAATCAAACCAGAGTATCAGTGGTTCAGTATTATCGTGAGAAGTACAACATTAGCCTAAAACAGGTGGCTTTGCCTGCCCTTCAAGCTGGCAATGATTCAAGGCCTGCTTATTTGCCCATGGAGCTTGCTTCAATTGTTCCCGGGCAGAGATACACGAAGAGGCTCAATGAAAAGCAAGTGACAAACGTGCTGAGAGCAACTTGCCAACGGCCCCGGGAGAGGGAATCCAATATACTGAAGATGGTGATGCACAATAATTACAAGGGAGAGGATCTTGTAAACAAAGAATTTGGAATACAAGTGAAAGAACAAATGGCACTGGTGGATGCTAGAATATTACCACCTCCAATGCTTAAGTATCATGAGAGTGGTCGTGAAACGAAGGATACTCCCAGGATGGGAGCATGGAACATGATCAATAAGAAAATGGTTAATGGTGCCAAAGTGGATTTTTGGGCTTTTGTCAATTTCTCTCGATCACGTGAAGATTTTAACTATCGCTTTTGTGAGGAACTCGTCTATATGTGCAACAGTAAAGGGGTGGAGTTCCATTCCGAGCCTTTACTTCCTGTACAGTCAGCTCATCCTAATCAAATTGAGAGGGTTCTGAGAGATATTCACAGGCAGTCAGTCCAGAGACTCAAGGATATGGCACAAGCCAGCAAACATCTTCAGTTGTTGATTATCATCTTGCCTGATGTCAAAGGATCTTATGGTCCCATTAAAAGAATTTGTGAAACAGAGCTTGGAATTGTCTCCCAGTGCTGTAATCCTAAGCAAGCACAAAAGCTTAGTAAGCAATACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGGTTGGTGGTAGAAACACAGTCTTAAATGATGCCATTCAGAGGAGGATTCCTCTTGTCAGTGATCGTCCTACAATCATCCTTGGAGCTGATGTCACTCATCCACAAGCTGGGGAAGACAGTAGCCCATCTATAGCAGCAGTGGTTGCCTCAATGGACTGGCCAGAGGTCTCCAAGTATAGAGGAATCCTGTCAGCACAGAATCATCGTCAAGAAATCATTCAAGATCTTTACAAATTAACTCACGATGAGAAAAGAGGGCCGGTTCATTCAGGAATGATTAGAGAACTTTTCATTGCATTCCGAAGATCGACTGGCCAAAAGCCGCACAGAATCATCTTCTATAGGGATGGGGTGAGTGAAGGGCAGTTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACGGAAGGCCTGTGCTTCTTTGGAGGAGGGGTATATGCCACCAGTTACTTTTGTTGTGGTGCAGAAAAGGCATCACACACGCCTTTTCCCTGCTGACAACCAGACAGATAGGAGTGGGAATATTCAACCAGGTACTGTGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTATCTTAATAGCCATGCAGGAATTCAGGGAACAAGTCGCCCAGCTCATTATCATGTCCTGTTTGATGAGAACAACTTCACTGCTGATGGCTTGCAGATTCTCACCAACAATCTGTGCTACACATATGCAAGGTGCACCCGCTCAGTTTCCATAGTGCCTCCTGCCTATTATGCGCATCTAGCTGCGTTTAGAGCACGCTATTACATTGAGGGCGATTCATCTGATGGTGCTTCTTCAGTTGGCAGAGGCAGCGGTGGCAGTGCTGCACAAACCCAGGTCCTTCCAAATGTGCACGAGTATGTCAAGGATGTGATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

993

Amino Acids

110.0

Weight (kDa)

9.61

Isoelectric Point (pI)

51.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 149 - 286 2.7e-37 N-terminal domain of argonaute
ArgoL1 PF08699 297 - 345 3.5e-18 Argonaute linker 1 domain
PAZ PF02170 373 - 476 5.7e-27 PAZ domain
ArgoL2 PF16488 486 - 533 3.2e-13 Argonaute linker 2 domain
ArgoMid PF16487 543 - 621 3.2e-09 Mid domain of argonaute
Piwi PF02171 640 - 954 7.4e-108 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 746
AbsI CCTCGAGG 1 cut(s) 924
Acc16I TGCGCA 2 cut(s) 1203, 2829
AccB1I GGYRCC 6 cut(s) 169, 187, 229, 310, 316, 1676
AccB7I CCANNNNNTGG 1 cut(s) 2183
AccBSI CCGCTC 1 cut(s) 2796
AccII CGCG 7 cut(s) 72, 93, 108, 114, 120, 176, 804
AclI AACGTT 1 cut(s) 1223
AclWI GGATC 5 cut(s) 721, 1031, 1113, 1518, 1954
AcoI YGGCCR 4 cut(s) 109, 115, 2234, 2389
AcsI RAATTY 5 cut(s) 938, 1526, 1968, 2645, 2676
AcuI CTGAAG 6 cut(s) 549, 1139, 1145, 1496, 1895, 2874
AcvI CACGTG 1 cut(s) 1721
AcyI GRCGYC 4 cut(s) 170, 188, 230, 311
AfaI GTAC 5 cut(s) 584, 1079, 1283, 1812, 2611
AflII CTTAAG 2 cut(s) 1592, 2060
AjnI CCWGG 4 cut(s) 1091, 1631, 2605, 2928
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 1968, 2000
Alw21I GWGCWC 4 cut(s) 244, 2792, 2851, 2949
Alw26I GTCTC 8 cut(s) 265, 597, 732, 824, 1870, 1999, 2061, 2248
Alw44I GTGCAC 2 cut(s) 2788, 2945
AlwI GGATC 5 cut(s) 721, 1031, 1113, 1518, 1954
AlwNI CAGNNNCTG 3 cut(s) 209, 296, 2612
Ama87I CYCGRG 3 cut(s) 924, 1379, 1452
ApaLI GTGCAC 2 cut(s) 2788, 2945
ApeKI GCWGC 7 cut(s) 790, 863, 1204, 2210, 2837, 2904, 2918
ApoI RAATTY 5 cut(s) 938, 1526, 1968, 2645, 2676
ArsI GACNNNNNNTTYG 2 cut(s) 923, 955
AseI ATTAAT 1 cut(s) 2067
Asp700I GAANNNNTTC 2 cut(s) 2292, 2729
AspLEI GCGC 7 cut(s) 172, 178, 190, 232, 313, 1204, 2830
AspS9I GGNCC 6 cut(s) 650, 920, 1449, 1955, 2333, 2932
AsuC2I CCSGG 7 cut(s) 135, 426, 649, 1380, 1381, 1453, 1454
AsuHPI GGTGA 5 cut(s) 941, 1060, 1495, 2440, 2749
AvaI CYCGRG 3 cut(s) 924, 1379, 1452
AvaII GGWCC 3 cut(s) 920, 1955, 2932
AxyI CCTNAGG 1 cut(s) 977
BaeGI GKGCMC 2 cut(s) 2792, 2949
BaeI ACNNNNGTAYC 6 cut(s) 1179, 1212, 1236, 1269, 2601, 2634
BalI TGGCCA 2 cut(s) 2236, 2391
BanI GGYRCC 6 cut(s) 169, 187, 229, 310, 316, 1676
BanII GRGCYC 1 cut(s) 277
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BauI CACGAG 2 cut(s) 237, 2948
BbrPI CACGTG 1 cut(s) 1721
BbsI GAAGAC 1 cut(s) 2196
Bbv12I GWGCWC 4 cut(s) 244, 2792, 2851, 2949
BbvI GCAGC 7 cut(s) 802, 850, 1216, 2222, 2824, 2905, 2916
BceAI ACGGC 2 cut(s) 389, 1463
BciT130I CCWGG 4 cut(s) 1093, 1633, 2607, 2930
BciVI GTATCC 2 cut(s) 1618, 2614
BclI TGATCA 1 cut(s) 1653
BcnI CCSGG 7 cut(s) 135, 426, 649, 1380, 1381, 1453, 1454
BcoDI GTCTC 8 cut(s) 265, 597, 732, 824, 1870, 1999, 2061, 2248
BfaI CTAG 2 cut(s) 1569, 2834
BfmI CTRYAG 3 cut(s) 788, 2205, 2416
BfoI RGCGCY 4 cut(s) 173, 191, 233, 314
BfrI CTTAAG 2 cut(s) 1592, 2060
BfuI GTATCC 2 cut(s) 1618, 2614
BglII AGATCT 2 cut(s) 496, 2299
BlpI GCTNAGC 1 cut(s) 243
Bme18I GGWCC 3 cut(s) 920, 1955, 2932
BmeT110I CYCGRG 3 cut(s) 924, 1379, 1452
BmgT120I GGNCC 6 cut(s) 650, 920, 1449, 1955, 2333, 2932
BmrI ACTGGG 1 cut(s) 1993
BmsI GCATC 8 cut(s) 511, 728, 1476, 1555, 2095, 2461, 2557, 2839
BmuI ACTGGG 1 cut(s) 1993
BpiI GAAGAC 1 cut(s) 2196
BplI GAGNNNNNCTC 4 cut(s) 259, 291, 2110, 2142
BpmI CTGGAG 5 cut(s) 180, 186, 273, 976, 1212
Bpu10I CCTNAGC 2 cut(s) 154, 2013
Bpu1102I GCTNAGC 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 1253, 1865
BpuMI CCSGG 7 cut(s) 135, 426, 649, 1380, 1381, 1453, 1454
BsaAI YACGTR 1 cut(s) 1721
BsaBI GATNNNNATC 4 cut(s) 501, 718, 1925, 2655
BsaHI GRCGYC 4 cut(s) 170, 188, 230, 311
BsaI GGTCTC 3 cut(s) 265, 824, 2248
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 4 cut(s) 2151, 2181, 2492, 2522
Bse118I RCCGGY 3 cut(s) 232, 313, 2335
Bse1I ACTGG 9 cut(s) 416, 959, 1195, 1216, 1563, 1999, 2237, 2392, 2519
Bse21I CCTNAGG 1 cut(s) 977
Bse3DI GCAATG 4 cut(s) 66, 653, 1336, 2367
Bse8I GATNNNNATC 4 cut(s) 501, 718, 1925, 2655
BseBI CCWGG 4 cut(s) 1093, 1633, 2607, 2930
BseJI GATNNNNATC 4 cut(s) 501, 718, 1925, 2655
BseMI GCAATG 4 cut(s) 66, 653, 1336, 2367
BseMII CTCAG 9 cut(s) 168, 257, 579, 684, 1001, 1148, 1421, 1838, 2811
BseNI ACTGG 9 cut(s) 416, 959, 1195, 1216, 1563, 1999, 2237, 2392, 2519
BseRI GAGGAG 2 cut(s) 2131, 2516
BseSI GKGCMC 2 cut(s) 2792, 2949
BseX3I CGGCCG 2 cut(s) 109, 115
BseXI GCAGC 7 cut(s) 802, 850, 1216, 2222, 2824, 2905, 2916
BseYI CCCAGC 2 cut(s) 2183, 2695
BsgI GTGCAG 3 cut(s) 849, 2558, 2904
Bsh1236I CGCG 7 cut(s) 72, 93, 108, 114, 120, 176, 804
Bsh1285I CGRYCG 2 cut(s) 112, 118
BshNI GGYRCC 6 cut(s) 169, 187, 229, 310, 316, 1676
BsiEI CGRYCG 2 cut(s) 112, 118
BsiHKAI GWGCWC 4 cut(s) 244, 2792, 2851, 2949
BsiHKCI CYCGRG 3 cut(s) 924, 1379, 1452
BslFI GGGAC 2 cut(s) 25, 1941
BsmAI GTCTC 8 cut(s) 265, 597, 732, 824, 1870, 1999, 2061, 2248
BsmFI GGGAC 2 cut(s) 25, 1941
BsmI GAATGC 2 cut(s) 615, 2372
Bso31I GGTCTC 3 cut(s) 265, 824, 2248
BsoBI CYCGRG 3 cut(s) 924, 1379, 1452
Bsp1286I GDGCHC 5 cut(s) 244, 277, 2792, 2851, 2949
Bsp1407I TGTACA 1 cut(s) 1810
Bsp1720I GCTNAGC 1 cut(s) 243
Bsp19I CCATGG 2 cut(s) 22, 1356
BspCNI CTCAG 9 cut(s) 167, 256, 580, 685, 1000, 1149, 1422, 1839, 2810
BspFNI CGCG 7 cut(s) 72, 93, 108, 114, 120, 176, 804
BspHI TCATGA 1 cut(s) 1600
BspMAI CTGCAG 1 cut(s) 792
BspPI GGATC 5 cut(s) 721, 1031, 1113, 1518, 1954
BspT107I GGYRCC 6 cut(s) 169, 187, 229, 310, 316, 1676
BspTI CTTAAG 2 cut(s) 1592, 2060
BspTNI GGTCTC 3 cut(s) 265, 824, 2248
BsrBI CCGCTC 1 cut(s) 2796
BsrDI GCAATG 4 cut(s) 66, 653, 1336, 2367
BsrFI RCCGGY 3 cut(s) 232, 313, 2335
BsrGI TGTACA 1 cut(s) 1810
BsrI ACTGG 9 cut(s) 416, 959, 1195, 1216, 1563, 1999, 2237, 2392, 2519
BssAI RCCGGY 3 cut(s) 232, 313, 2335
BssNI GRCGYC 4 cut(s) 170, 188, 230, 311
BssSI CACGAG 2 cut(s) 237, 2948
BssT1I CCWWGG 4 cut(s) 22, 399, 1356, 2155
Bst2BI CACGAG 2 cut(s) 237, 2948
Bst2UI CCWGG 4 cut(s) 1093, 1633, 2607, 2930
Bst4CI ACNGT 6 cut(s) 884, 1772, 1815, 2095, 2195, 2614
Bst6I CTCTTC 5 cut(s) 118, 363, 626, 670, 1391
BstACI GRCGYC 4 cut(s) 170, 188, 230, 311
BstAFI CTTAAG 2 cut(s) 1592, 2060
BstAPI GCANNNNNTGC 1 cut(s) 2219
BstAUI TGTACA 1 cut(s) 1810
BstBAI YACGTR 1 cut(s) 1721
BstDSI CCRYGG 5 cut(s) 22, 106, 112, 118, 1356
BstEII GGTNACC 1 cut(s) 929
BstENI CCTNNNNNAGG 1 cut(s) 1298
BstFNI CGCG 7 cut(s) 72, 93, 108, 114, 120, 176, 804
BstH2I RGCGCY 4 cut(s) 173, 191, 233, 314
BstHHI GCGC 7 cut(s) 172, 178, 190, 232, 313, 1204, 2830
BstMAI GTCTC 8 cut(s) 265, 597, 732, 824, 1870, 1999, 2061, 2248
BstMCI CGRYCG 2 cut(s) 112, 118
BstNI CCWGG 4 cut(s) 1093, 1633, 2607, 2930
BstPI GGTNACC 1 cut(s) 929
BstSFI CTRYAG 3 cut(s) 788, 2205, 2416
BstSLI GKGCMC 2 cut(s) 2792, 2949
BstUI CGCG 7 cut(s) 72, 93, 108, 114, 120, 176, 804
BstV1I GCAGC 7 cut(s) 802, 850, 1216, 2222, 2824, 2905, 2916
BstV2I GAAGAC 1 cut(s) 2196
BstX2I RGATCY 5 cut(s) 496, 713, 1510, 1946, 2299
BstYI RGATCY 5 cut(s) 496, 713, 1510, 1946, 2299
BstZI CGGCCG 2 cut(s) 109, 115
Bsu36I CCTNAGG 1 cut(s) 977
BsuI GTATCC 2 cut(s) 1618, 2614
BtgI CCRYGG 5 cut(s) 22, 106, 112, 118, 1356
BtsI GCAGTG 4 cut(s) 642, 2220, 2733, 2920
BtsIMutI CAGTG 9 cut(s) 423, 642, 1263, 1556, 2006, 2140, 2220, 2733, 2920
CaiI CAGNNNCTG 3 cut(s) 209, 296, 2612
CciI TCATGA 1 cut(s) 1600
CciNI GCGGCCGC 2 cut(s) 109, 115
CfoI GCGC 7 cut(s) 172, 178, 190, 232, 313, 1204, 2830
Cfr10I RCCGGY 3 cut(s) 232, 313, 2335
Cfr13I GGNCC 6 cut(s) 650, 920, 1449, 1955, 2333, 2932
Cfr42I CCGCGG 3 cut(s) 109, 115, 121
Cfr9I CCCGGG 2 cut(s) 1379, 1452
CsiI ACCWGGT 1 cut(s) 2605
Csp6I GTAC 5 cut(s) 583, 1078, 1282, 1811, 2610
CspCI CAANNNNNGTGG 2 cut(s) 2062, 2097
CviAII CATG 8 cut(s) 23, 628, 1357, 1601, 1644, 1651, 2669, 2708
CviQI GTAC 5 cut(s) 583, 1078, 1282, 1811, 2610
DinI GGCGCC 4 cut(s) 171, 189, 231, 312
DrdI GACNNNNNNGTC 1 cut(s) 746
DseDI GACNNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 4 cut(s) 109, 115, 2234, 2389
EagI CGGCCG 2 cut(s) 109, 115
Eam1104I CTCTTC 5 cut(s) 118, 363, 626, 670, 1391
EarI CTCTTC 5 cut(s) 118, 363, 626, 670, 1391
EciI GGCGGA 1 cut(s) 114
EclXI CGGCCG 2 cut(s) 109, 115
Eco130I CCWWGG 4 cut(s) 22, 399, 1356, 2155
Eco147I AGGCCT 2 cut(s) 1342, 2486
Eco24I GRGCYC 1 cut(s) 277
Eco31I GGTCTC 3 cut(s) 265, 824, 2248
Eco47I GGWCC 3 cut(s) 920, 1955, 2932
Eco52I CGGCCG 2 cut(s) 109, 115
Eco57I CTGAAG 6 cut(s) 549, 1139, 1145, 1496, 1895, 2874
Eco72I CACGTG 1 cut(s) 1721
Eco81I CCTNAGG 1 cut(s) 977
Eco88I CYCGRG 3 cut(s) 924, 1379, 1452
Eco91I GGTNACC 1 cut(s) 929
EcoNI CCTNNNNNAGG 1 cut(s) 1298
EcoO109I RGGNCCY 2 cut(s) 920, 2932
EcoO65I GGTNACC 1 cut(s) 929
EcoRI GAATTC 1 cut(s) 2676
EcoRII CCWGG 4 cut(s) 1091, 1631, 2605, 2928
EcoT14I CCWWGG 4 cut(s) 22, 399, 1356, 2155
EcoT38I GRGCYC 1 cut(s) 277
EgeI GGCGCC 4 cut(s) 171, 189, 231, 312
EheI GGCGCC 4 cut(s) 171, 189, 231, 312
ErhI CCWWGG 4 cut(s) 22, 399, 1356, 2155
FaeI CATG 8 cut(s) 26, 631, 1360, 1604, 1647, 1654, 2672, 2711
FalI AAGNNNNNCTT 4 cut(s) 360, 392, 1330, 1362
FaqI GGGAC 2 cut(s) 25, 1941
FatI CATG 8 cut(s) 22, 627, 1356, 1600, 1643, 1650, 2668, 2707
FauI CCCGC 2 cut(s) 181, 2801
FauNDI CATATG 2 cut(s) 619, 2779
FbaI TGATCA 1 cut(s) 1653
FriOI GRGCYC 1 cut(s) 277
FspAI RTGCGCAY 1 cut(s) 2829
FspBI CTAG 2 cut(s) 1569, 2834
FspI TGCGCA 2 cut(s) 1203, 2829
GlaI GCGC 7 cut(s) 171, 177, 189, 231, 312, 1203, 2829
GsaI CCCAGC 2 cut(s) 2187, 2699
GsuI CTGGAG 5 cut(s) 180, 186, 273, 976, 1212
HaeII RGCGCY 4 cut(s) 173, 191, 233, 314
HhaI GCGC 7 cut(s) 172, 178, 190, 232, 313, 1204, 2830
Hin1I GRCGYC 4 cut(s) 170, 188, 230, 311
Hin1II CATG 8 cut(s) 26, 631, 1360, 1604, 1647, 1654, 2672, 2711
Hin6I GCGC 7 cut(s) 170, 176, 188, 230, 311, 1202, 2828
HinP1I GCGC 7 cut(s) 170, 176, 188, 230, 311, 1202, 2828
HincII GTYRAC 1 cut(s) 1221
HindII GTYRAC 1 cut(s) 1221
HindIII AAGCTT 2 cut(s) 374, 2027
HpaI GTTAAC 1 cut(s) 1221
HphI GGTGA 5 cut(s) 941, 1060, 1495, 2440, 2749
Hpy166II GTNNAC 5 cut(s) 1221, 1227, 1519, 2790, 2947
Hpy8I GTNNAC 5 cut(s) 1221, 1227, 1519, 2790, 2947
Hpy99I CGWCG 2 cut(s) 268, 394
HpyAV CCTTC 8 cut(s) 670, 695, 720, 1328, 1615, 2429, 2476, 2945
HpyCH4III ACNGT 6 cut(s) 884, 1772, 1815, 2095, 2195, 2614
HpyCH4IV ACGT 3 cut(s) 1223, 1425, 1720
HpySE526I ACGT 3 cut(s) 1223, 1425, 1720
Hsp92I GRCGYC 4 cut(s) 170, 188, 230, 311
Hsp92II CATG 8 cut(s) 26, 631, 1360, 1604, 1647, 1654, 2672, 2711
HspAI GCGC 7 cut(s) 170, 176, 188, 230, 311, 1202, 2828
KasI GGCGCC 4 cut(s) 169, 187, 229, 310
KroI GCCGGC 2 cut(s) 232, 313
KroNI GCCGGC 2 cut(s) 234, 315
Ksp22I TGATCA 1 cut(s) 1653
KspAI GTTAAC 1 cut(s) 1221
KspI CCGCGG 3 cut(s) 109, 115, 121
Lsp1109I GCAGC 7 cut(s) 802, 850, 1216, 2222, 2824, 2905, 2916
LweI GCATC 8 cut(s) 511, 728, 1476, 1555, 2095, 2461, 2557, 2839
MabI ACCWGGT 1 cut(s) 2605
MaeI CTAG 2 cut(s) 1569, 2834
MaeII ACGT 3 cut(s) 1223, 1425, 1720
MaeIII GTNAC 6 cut(s) 929, 1048, 1150, 1417, 2167, 2521
MbiI CCGCTC 1 cut(s) 2796
MfeI CAATTG 1 cut(s) 1371
MflI RGATCY 5 cut(s) 496, 713, 1510, 1946, 2299
MhlI GDGCHC 5 cut(s) 244, 277, 2792, 2851, 2949
MlsI TGGCCA 2 cut(s) 2236, 2391
MluNI TGGCCA 2 cut(s) 2236, 2391
Mly113I GGCGCC 4 cut(s) 170, 188, 230, 311
MlyI GAGTC 3 cut(s) 459, 599, 1872
MmeI TCCRAC 2 cut(s) 1083, 2663
Mox20I TGGCCA 2 cut(s) 2236, 2391
MroNI GCCGGC 2 cut(s) 232, 313
MroXI GAANNNNTTC 2 cut(s) 2292, 2729
MscI TGGCCA 2 cut(s) 2236, 2391
MslI CAYNNNNRTG 6 cut(s) 59, 507, 510, 1605, 2706, 2952
Msp20I TGGCCA 2 cut(s) 2236, 2391
MspA1I CMGCKG 5 cut(s) 108, 114, 120, 571, 2907
MspCI CTTAAG 2 cut(s) 1592, 2060
MunI CAATTG 1 cut(s) 1371
Mva1269I GAATGC 2 cut(s) 615, 2372
MvaI CCWGG 4 cut(s) 1093, 1633, 2607, 2930
MvnI CGCG 7 cut(s) 72, 93, 108, 114, 120, 176, 804
NaeI GCCGGC 2 cut(s) 234, 315
NarI GGCGCC 4 cut(s) 170, 188, 230, 311
NciI CCSGG 7 cut(s) 135, 426, 649, 1380, 1381, 1453, 1454
NcoI CCATGG 2 cut(s) 22, 1356
NdeI CATATG 2 cut(s) 619, 2779
NgoMIV GCCGGC 2 cut(s) 232, 313
NlaIII CATG 8 cut(s) 26, 631, 1360, 1604, 1647, 1654, 2672, 2711
NmuCI GTSAC 4 cut(s) 929, 1048, 1417, 2167
NotI GCGGCCGC 2 cut(s) 109, 115
NsbI TGCGCA 2 cut(s) 1203, 2829
PaeR7I CTCGAG 1 cut(s) 924
PagI TCATGA 1 cut(s) 1600
PceI AGGCCT 2 cut(s) 1342, 2486
PctI GAATGC 2 cut(s) 615, 2372
PdiI GCCGGC 2 cut(s) 234, 315
PdmI GAANNNNTTC 2 cut(s) 2292, 2729
PflMI CCANNNNNTGG 1 cut(s) 2183
PleI GAGTC 3 cut(s) 458, 598, 1872
PluTI GGCGCC 4 cut(s) 173, 191, 233, 314
PmaCI CACGTG 1 cut(s) 1721
PmlI CACGTG 1 cut(s) 1721
PpsI GAGTC 3 cut(s) 458, 598, 1872
Ppu21I YACGTR 1 cut(s) 1721
PpuMI RGGWCCY 2 cut(s) 920, 2932
PshBI ATTAAT 1 cut(s) 2067
Psp1406I AACGTT 1 cut(s) 1223
Psp5II RGGWCCY 2 cut(s) 920, 2932
Psp6I CCWGG 4 cut(s) 1091, 1631, 2605, 2928
PspCI CACGTG 1 cut(s) 1721
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 2 cut(s) 2183, 2695
PspGI CCWGG 4 cut(s) 1091, 1631, 2605, 2928
PspPI GGNCC 6 cut(s) 650, 920, 1449, 1955, 2333, 2932
PspPPI RGGWCCY 2 cut(s) 920, 2932
PspXI VCTCGAGB 1 cut(s) 924
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PstI CTGCAG 1 cut(s) 792
PstNI CAGNNNCTG 3 cut(s) 209, 296, 2612
PsuI RGATCY 5 cut(s) 496, 713, 1510, 1946, 2299
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 5 cut(s) 584, 1079, 1283, 1812, 2611
RsaNI GTAC 5 cut(s) 583, 1078, 1282, 1811, 2610
RseI CAYNNNNRTG 6 cut(s) 59, 507, 510, 1605, 2706, 2952
SacII CCGCGG 3 cut(s) 109, 115, 121
Sau96I GGNCC 6 cut(s) 650, 920, 1449, 1955, 2333, 2932
SchI GAGTC 3 cut(s) 459, 599, 1872
SduI GDGCHC 5 cut(s) 244, 277, 2792, 2851, 2949
SexAI ACCWGGT 1 cut(s) 2605
SfaNI GCATC 8 cut(s) 511, 728, 1476, 1555, 2095, 2461, 2557, 2839
SfcI CTRYAG 3 cut(s) 788, 2205, 2416
SfoI GGCGCC 4 cut(s) 171, 189, 231, 312
Sfr274I CTCGAG 1 cut(s) 924
Sfr303I CCGCGG 3 cut(s) 109, 115, 121
SgrBI CCGCGG 3 cut(s) 109, 115, 121
SinI GGWCC 3 cut(s) 920, 1955, 2932
SlaI CTCGAG 1 cut(s) 924
SmaI CCCGGG 2 cut(s) 1381, 1454
SmiMI CAYNNNNRTG 6 cut(s) 59, 507, 510, 1605, 2706, 2952
SmlI CTYRAG 5 cut(s) 924, 1232, 1592, 1880, 2060
SmoI CTYRAG 5 cut(s) 924, 1232, 1592, 1880, 2060
SseBI AGGCCT 2 cut(s) 1342, 2486
SspDI GGCGCC 4 cut(s) 169, 187, 229, 310
SspI AATATT 3 cut(s) 1009, 1575, 2599
SspMI CTAG 2 cut(s) 1569, 2834
StuI AGGCCT 2 cut(s) 1342, 2486
StyI CCWWGG 4 cut(s) 22, 399, 1356, 2155
TaaI ACNGT 6 cut(s) 884, 1772, 1815, 2095, 2195, 2614
TaiI ACGT 3 cut(s) 1226, 1428, 1723
TaqI TCGA 7 cut(s) 266, 480, 706, 925, 1183, 1714, 2384
TatI WGTACW 3 cut(s) 582, 1281, 1810
TscAI CASTG 9 cut(s) 423, 649, 1263, 1563, 2006, 2140, 2220, 2740, 2920
TseFI GTSAC 4 cut(s) 929, 1048, 1417, 2167
TseI GCWGC 7 cut(s) 790, 863, 1204, 2210, 2837, 2904, 2918
Tsp45I GTSAC 4 cut(s) 929, 1048, 1417, 2167
TspDTI ATGAA 7 cut(s) 69, 315, 644, 1422, 2330, 2356, 2862
TspGWI ACGGA 2 cut(s) 378, 2494
TspMI CCCGGG 2 cut(s) 1379, 1452
TspRI CASTG 9 cut(s) 423, 649, 1263, 1563, 2006, 2140, 2220, 2740, 2920
Van91I CCANNNNNTGG 1 cut(s) 2183
Vha464I CTTAAG 2 cut(s) 1592, 2060
VneI GTGCAC 2 cut(s) 2788, 2945
VpaK11BI GGWCC 3 cut(s) 920, 1955, 2932
VspI ATTAAT 1 cut(s) 2067
XagI CCTNNNNNAGG 1 cut(s) 1298
XapI RAATTY 5 cut(s) 938, 1526, 1968, 2645, 2676
XcmI CCANNNNNNNNNTGG 2 cut(s) 472, 1255
XhoI CTCGAG 1 cut(s) 924
XmaI CCCGGG 2 cut(s) 1379, 1452
XmnI GAANNNNTTC 2 cut(s) 2292, 2729
XspI CTAG 2 cut(s) 1569, 2834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.