Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001300

Physical Location & Seq

Genomic Coordinates Forward (+)

14621316 .. 14626559

Gene Structure 5244 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1152400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGATAATATTATTATGTCTTTCGTGTCTTATGGATGTGGGGGTGTTAATTACCCCTGATGTTCCAGTGAAAAGGGTGAAAAGGGATGTGATTAAGCAGCTCGTTGTTTTGTACAAAGAGTCACACTTAGGTCAAAGAACCCCAGCGTATGATGGTATGAAAAGCATTTACACAGCAGGTCCATTGCCTTTTGAATCCAAAGAATTTCTAATCAAGCTACCTGAGAAAGATTACTGTGACGGGTCATCTTCTTCTTCTCGCTCGAAAAGGTTGGATAGGGAATTTAAGGTGACTCTCAAACTGGGGAACAAACCCGACCTTTATCAACTACAACAATTCTTGCGCAGGAAGAAACACGAGTTGCCTCAAGAGGCTATACAAGCACTTGATGTTGCTCTTAGAGCCTCACCATCTGAAAAGCCTTTTTTCTCCACAACGCTTGGGCCAAAAGGCGAGCTATGTGAGTCTTGTGATCTTTATGCTCAGACCTATTTTCAGTTTGAATCAGTACTTATTATACGAACTCTGAATATTAGGTTTGCTCTAGAAGACGGAAGTAGTAAGACACTAGTTCAATATTATCGTGAAAGGTACAATGTTGTGCTAAAGAATGTGGCGATGCCTGCCATTCAATCTGGAAGTGCTTCCAAACCTGTTTATTTGCCCATGGAGCTTTGTACAATTGTTTCTGGCCAGAGATACTCCAGGAAGCTTAATGAAAGACAAGTAACAGCCTTACTACGAGCTACATGTCAACGTCCCGGTGATAGAGAGAGAGTATACATCAGGTCTACTCCTATGTCGTCTCTACAATGGATGCTAAAATACCATGACAGTGGTCGTGAAAAGTTTGCAAATCCATCGATGGGGCAATGGAACATGACGAACAAGAAATTGGTTAACGGTGGTAGAGTAGATTTCTGGGGTTTTGTCAATTTCTCCCGGTTGGACCAGAATTTTAACTACCGATTCTGTGAAGATTTGGTCAATACGTGCATCAGCAAGGGAGTGCATTTCAATGTGGAACCTTTAGTACCGTTCCAATCAGCTCATCACGGACAAATGGAGAGCGTGCTAAGAGATATTCATAGGACGTCTATCAAGCGACTTGAAGAAATTGGACAGAAGGGAAGGCATCTTCAGTTGTTGATCGTCATTTTGCCTGACGTTGGCGGAAGGAATACTATTCTGAATGATGCCGTTCAAGAGAGGAATCCCTTTTTTACTGGCGATGCCACAATCATCTTTGGTGCGGATGTTACTCATCCTCCACCTGGGGAGGACAGCAGCCTGTCAATTGCAGCTCATCCCGACAAGCAGGAGCCCGACAAACAACTAAAGTGCCAACTCCTCAGGAAGCTGTATGCTCGAGCCAGGGACCATTCCCAGCGAAATTCCTGCCCGAACACAAAGTACCGAGGAATTGTTTCTGCACGAGCTCACCGTGAAGAAATAATCCAAAATCTTTATACTTCAAGGCAGGACAATAAGAATACTGTTGTTCATGGAGGAATGATCAGAGATGGAGTTAGTGAAGGCCAATTTAGTCAAGTTCTGCTGCATGAAGTGGATGCAATACGACGTGCTTGTGCCTCGCTGGAGAAGGGATGCATGCCACCAGTTACTTTCGTAGTGGTGCAGAAAAGACATCATACTCGGCTGTTCCCTACTGATCGGAAGGCAGATAAGAGTGGCAATATTCTACCAGGCACTGTCGTTGATACTACGATTTGTCACCCGACGGAGTTTGATTTTATCTCAACAGTCATGCTGGAATTCAGGTACTGTCTGTACGGAACTAGCCGGCCAGCGCATTACCATGTCTTTTTCGATGAGAACAGGTTCACTGCCGATGCCTTGCAAGTATTAACAAACAACTTATGTTACACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002252 GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009814 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016441 GO:0016458 GO:0019222 GO:0035821 GO:0040029 GO:0043207 GO:0044003 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0048519 GO:0050789 GO:0050896 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0098542 GO:0098586

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

631

Amino Acids

71.82

Weight (kDa)

9.16

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgoN	PF16486	15 - 133	3.6e-23	N-terminal domain of argonaute
PAZ	PF02170	177 - 246	5.3e-17	PAZ domain
ArgoMid	PF16487	286 - 364	2.2e-08	Mid domain of argonaute
Piwi	PF02171	382 - 630	6.1e-46	Piwi domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27880
fragaria_vesca	FvH4_3g30201 FvH4_3g30202
malus_domestica	MD03G1130600.v1.1 MD03G1254500.v1.1 MD11G1152200.v1.1 MD11G1152400.v1.1
prunus_persica	Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1 Prupe.6G115400_v2.0.a1
pyrus_communis	pycom01g05040 pycom11g12470
rosa_chinensis	RchiOBHm_Chr1g0362621 RchiOBHm_Chr5g0055991 RchiOBHm_Chr5g0056001 RchiOBHm_Chr5g0056021 RchiOBHm_Chr5g0056031 RchiOBHm_Chr5g0056051 RchiOBHm_Chr5g0056071 RchiOBHm_Chr5g0056081 RchiOBHm_Chr5g0056091
rosa_laevigata	RLG00000027633 RLG00000035041 RLG00000035044 RLG00000035045 RLG00000035046
rosa_roxburghii	Rroxscaffold_1G00024210 Rroxscaffold_1G00024220
rosa_rugosa	Rorug05G0297000 Rorug05G0297100 Rorug05G0297200 Rorug05G0297300 Rorug05G0297300 Rorug05G0297400
rosa_samantha	Rh1CG289700 Rh1DG302900 Rh5AG368000 Rh5AG368200 Rh5AG368300 Rh5CG401800 Rh5CG402100 Rh5CG402200 Rh5DG391500 Rh5DG391600 Rh5DG391700
rosa_wichuraiana	Rw0G004500 Rw1G027430 Rw5G034540 Rw5G034570 Rw5G034580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1097
Acc16I	TGCGCA	1 cut(s)	344
Acc36I	ACCTGC	1 cut(s)	167
AccI	GTMKAC	2 cut(s)	780, 791
AciI	CCGC	2 cut(s)	1173, 1253
AcoI	YGGCCR	2 cut(s)	691, 1805
AcsI	RAATTY	5 cut(s)	203, 281, 955, 1393, 1775
AcuI	CTGAAG	1 cut(s)	1124
AcyI	GRCGYC	1 cut(s)	1094
AfaI	GTAC	8 cut(s)	113, 510, 593, 679, 1035, 1415, 1784, 1793
AfiI	CCNNNNNNNGG	3 cut(s)	866, 1169, 1274
AflIII	ACRYGT	2 cut(s)	749, 1887
AgsI	TTSAA	8 cut(s)	194, 503, 575, 632, 1018, 1112, 1205, 1476
AhlI	ACTAGT	1 cut(s)	568
AjiI	CACGTC	1 cut(s)	1583
AjnI	CCWGG	4 cut(s)	704, 1273, 1373, 1705
AjuI	GAANNNNNNNTTGG	2 cut(s)	878, 910
Alw21I	GWGCWC	1 cut(s)	1441
Alw26I	GTCTC	1 cut(s)	810
AlwNI	CAGNNNCTG	2 cut(s)	1712, 1785
Ama87I	CYCGRG	1 cut(s)	1368
AoxI	GGCC	4 cut(s)	443, 691, 1537, 1805
ApeKI	GCWGC	4 cut(s)	97, 1287, 1301, 1558
ApoI	RAATTY	5 cut(s)	203, 281, 955, 1393, 1775
Asp700I	GAANNNNTTC	3 cut(s)	643, 1426, 1841
AspLEI	GCGC	2 cut(s)	345, 1813
AspS9I	GGNCC	4 cut(s)	179, 443, 949, 1378
AsuC2I	CCSGG	2 cut(s)	762, 943
AsuHPI	GGTGA	6 cut(s)	88, 301, 399, 776, 1433, 1727
AvaI	CYCGRG	1 cut(s)	1368
AvaII	GGWCC	3 cut(s)	179, 949, 1378
AxyI	CCTNAGG	1 cut(s)	1352
BalI	TGGCCA	1 cut(s)	693
BanII	GRGCYC	2 cut(s)	1326, 1441
BauI	CACGAG	2 cut(s)	356, 1434
BbsI	GAAGAC	1 cut(s)	555
Bbv12I	GWGCWC	1 cut(s)	1441
BbvI	GCAGC	4 cut(s)	109, 1299, 1313, 1545
BccI	CCATC	5 cut(s)	146, 418, 859, 868, 1517
BceAI	ACGGC	1 cut(s)	1184
BcgI	CGANNNNNNTGC	4 cut(s)	843, 877, 1380, 1414
BciT130I	CCWGG	4 cut(s)	706, 1275, 1375, 1707
BclI	TGATCA	1 cut(s)	1515
BcnI	CCSGG	2 cut(s)	762, 943
BcoDI	GTCTC	1 cut(s)	810
BcuI	ACTAGT	1 cut(s)	568
BfaI	CTAG	3 cut(s)	545, 569, 1800
BfuAI	ACCTGC	1 cut(s)	167
BisI	GCNGC	4 cut(s)	98, 1288, 1302, 1559
BlsI	GCNGC	4 cut(s)	99, 1289, 1303, 1560
BmcAI	AGTACT	1 cut(s)	510
Bme1390I	CCNGG	6 cut(s)	706, 762, 943, 1275, 1375, 1707
Bme18I	GGWCC	3 cut(s)	179, 949, 1378
BmeT110I	CYCGRG	1 cut(s)	1368
BmgBI	CACGTC	1 cut(s)	1583
BmgT120I	GGNCC	4 cut(s)	179, 443, 949, 1378
BmiI	GGNNCC	3 cut(s)	1026, 1323, 1379
BmrFI	CCNGG	6 cut(s)	706, 762, 943, 1275, 1375, 1707
BmrI	ACTGGG	1 cut(s)	311
BmsI	GCATC	9 cut(s)	609, 807, 1005, 1144, 1186, 1222, 1561, 1598, 1843
BmuI	ACTGGG	1 cut(s)	311
BoxI	GACNNNNGTC	1 cut(s)	837
BpiI	GAAGAC	1 cut(s)	555
BpmI	CTGGAG	2 cut(s)	688, 1619
BpuEI	CTTGAG	1 cut(s)	351
BpuMI	CCSGG	2 cut(s)	762, 943
Bsa29I	ATCGAT	1 cut(s)	863
BsaAI	YACGTR	2 cut(s)	993, 1890
BsaHI	GRCGYC	1 cut(s)	1094
BsaJI	CCNNGG	4 cut(s)	666, 1274, 1374, 1417
BsaXI	ACNNNNNCTCC	2 cut(s)	1252, 1282
Bsc4I	CCNNNNNNNGG	3 cut(s)	866, 1169, 1274
Bse118I	RCCGGY	1 cut(s)	1803
Bse1I	ACTGG	4 cut(s)	65, 306, 1231, 1619
Bse21I	CCTNAGG	1 cut(s)	1352
Bse3DI	GCAATG	2 cut(s)	182, 878
BseBI	CCWGG	4 cut(s)	706, 1275, 1375, 1707
BseCI	ATCGAT	1 cut(s)	863
BseDI	CCNNGG	4 cut(s)	666, 1274, 1374, 1417
BseGI	GGATG	8 cut(s)	40, 91, 822, 1261, 1264, 1306, 1576, 1613
BseLI	CCNNNNNNNGG	3 cut(s)	866, 1169, 1274
BseMI	GCAATG	2 cut(s)	182, 878
BseMII	CTCAG	3 cut(s)	213, 497, 1366
BseNI	ACTGG	4 cut(s)	65, 306, 1231, 1619
BseRI	GAGGAG	1 cut(s)	1340
BseXI	GCAGC	4 cut(s)	109, 1299, 1313, 1545
BseYI	CCCAGC	2 cut(s)	142, 1386
BsgI	GTGCAG	2 cut(s)	1416, 1658
BshFI	GGCC	4 cut(s)	445, 693, 1539, 1807
BshVI	ATCGAT	1 cut(s)	863
BsiHKAI	GWGCWC	1 cut(s)	1441
BsiHKCI	CYCGRG	1 cut(s)	1368
BsiSI	CCGG	3 cut(s)	762, 943, 1804
BslFI	GGGAC	2 cut(s)	744, 1391
BslI	CCNNNNNNNGG	3 cut(s)	866, 1169, 1274
BsmAI	GTCTC	1 cut(s)	810
BsmBI	CGTCTC	1 cut(s)	810
BsmFI	GGGAC	2 cut(s)	744, 1391
BsnI	GGCC	4 cut(s)	445, 693, 1539, 1807
BsoBI	CYCGRG	1 cut(s)	1368
Bsp1286I	GDGCHC	2 cut(s)	1326, 1441
Bsp1407I	TGTACA	2 cut(s)	111, 677
Bsp143I	GATC	4 cut(s)	472, 1149, 1515, 1672
Bsp19I	CCATGG	1 cut(s)	666
BspACI	CCGC	2 cut(s)	1173, 1253
BspANI	GGCC	4 cut(s)	445, 693, 1539, 1807
BspCNI	CTCAG	3 cut(s)	214, 496, 1365
BspDI	ATCGAT	1 cut(s)	863
BspLI	GGNNCC	3 cut(s)	1026, 1323, 1379
BspMI	ACCTGC	1 cut(s)	167
BsrDI	GCAATG	2 cut(s)	182, 878
BsrFI	RCCGGY	1 cut(s)	1803
BsrGI	TGTACA	2 cut(s)	111, 677
BsrI	ACTGG	4 cut(s)	65, 306, 1231, 1619
BssAI	RCCGGY	1 cut(s)	1803
BssECI	CCNNGG	4 cut(s)	666, 1274, 1374, 1417
BssMI	GATC	4 cut(s)	472, 1149, 1515, 1672
BssNAI	GTATAC	1 cut(s)	781
BssNI	GRCGYC	1 cut(s)	1094
BssSI	CACGAG	2 cut(s)	356, 1434
BssT1I	CCWWGG	1 cut(s)	666
Bst1107I	GTATAC	1 cut(s)	781
Bst2BI	CACGAG	2 cut(s)	356, 1434
Bst2UI	CCWGG	4 cut(s)	706, 1275, 1375, 1707
Bst4CI	ACNGT	9 cut(s)	236, 836, 905, 1038, 1445, 1498, 1714, 1765, 1787
BstACI	GRCGYC	1 cut(s)	1094
BstAUI	TGTACA	2 cut(s)	111, 677
BstBAI	YACGTR	2 cut(s)	993, 1890
BstC8I	GCNNGC	6 cut(s)	455, 624, 1073, 1613, 1805, 1809
BstDEI	CTNAG	6 cut(s)	127, 222, 398, 483, 1076, 1352
BstDSI	CCRYGG	1 cut(s)	666
BstF5I	GGATG	8 cut(s)	40, 91, 822, 1261, 1264, 1306, 1576, 1613
BstHHI	GCGC	2 cut(s)	345, 1813
BstKTI	GATC	4 cut(s)	475, 1152, 1518, 1675
BstMAI	GTCTC	1 cut(s)	810
BstMBI	GATC	4 cut(s)	472, 1149, 1515, 1672
BstMWI	GCNNNNNNNGC	4 cut(s)	380, 401, 623, 670
BstNI	CCWGG	4 cut(s)	706, 1275, 1375, 1707
BstNSI	RCATGY	2 cut(s)	753, 1615
BstPAI	GACNNNNGTC	1 cut(s)	837
BstSCI	CCNGG	6 cut(s)	704, 760, 941, 1273, 1373, 1705
BstV1I	GCAGC	4 cut(s)	109, 1299, 1313, 1545
BstV2I	GAAGAC	1 cut(s)	555
BstXI	CCANNNNNNTGG	1 cut(s)	836
BstZ17I	GTATAC	1 cut(s)	781
Bsu15I	ATCGAT	1 cut(s)	863
Bsu36I	CCTNAGG	1 cut(s)	1352
BsuRI	GGCC	4 cut(s)	445, 693, 1539, 1807
BsuTUI	ATCGAT	1 cut(s)	863
BtgI	CCRYGG	1 cut(s)	666
BtgZI	GCGATG	2 cut(s)	632, 1245
BtrI	CACGTC	1 cut(s)	1583
BtsCI	GGATG	8 cut(s)	40, 91, 822, 1261, 1264, 1306, 1576, 1613
BtsI	GCAGTG	1 cut(s)	1845
BtsIMutI	CAGTG	4 cut(s)	72, 841, 1710, 1845
BveI	ACCTGC	1 cut(s)	167
Cac8I	GCNNGC	6 cut(s)	455, 624, 1073, 1613, 1805, 1809
CaiI	CAGNNNCTG	2 cut(s)	1712, 1785
CfoI	GCGC	2 cut(s)	345, 1813
Cfr10I	RCCGGY	1 cut(s)	1803
Cfr13I	GGNCC	4 cut(s)	179, 443, 949, 1378
ClaI	ATCGAT	1 cut(s)	863
Csp6I	GTAC	8 cut(s)	112, 509, 592, 678, 1034, 1414, 1783, 1792
CspCI	CAANNNNNGTGG	2 cut(s)	421, 456
CviAII	CATG	9 cut(s)	667, 750, 830, 880, 1505, 1562, 1612, 1768, 1820
CviQI	GTAC	8 cut(s)	112, 509, 592, 678, 1034, 1414, 1783, 1792
DdeI	CTNAG	6 cut(s)	127, 222, 398, 483, 1076, 1352
DpnI	GATC	4 cut(s)	474, 1151, 1517, 1674
DpnII	GATC	4 cut(s)	472, 1149, 1515, 1672
EaeI	YGGCCR	2 cut(s)	691, 1805
EciI	GGCGGA	1 cut(s)	1188
Ecl136II	GAGCTC	1 cut(s)	1439
Eco130I	CCWWGG	1 cut(s)	666
Eco24I	GRGCYC	2 cut(s)	1326, 1441
Eco47I	GGWCC	3 cut(s)	179, 949, 1378
Eco53kI	GAGCTC	1 cut(s)	1439
Eco57I	CTGAAG	1 cut(s)	1124
Eco81I	CCTNAGG	1 cut(s)	1352
Eco88I	CYCGRG	1 cut(s)	1368
EcoICRI	GAGCTC	1 cut(s)	1439
EcoRI	GAATTC	1 cut(s)	1775
EcoRII	CCWGG	4 cut(s)	704, 1273, 1373, 1705
EcoT14I	CCWWGG	1 cut(s)	666
EcoT22I	ATGCAT	1 cut(s)	1613
EcoT38I	GRGCYC	2 cut(s)	1326, 1441
ErhI	CCWWGG	1 cut(s)	666
Esp3I	CGTCTC	1 cut(s)	810
FaeI	CATG	9 cut(s)	670, 753, 833, 883, 1508, 1565, 1615, 1771, 1823
FaqI	GGGAC	2 cut(s)	744, 1391
FatI	CATG	9 cut(s)	666, 749, 829, 879, 1504, 1561, 1611, 1767, 1819
FbaI	TGATCA	1 cut(s)	1515
FblI	GTMKAC	2 cut(s)	780, 791
Fnu4HI	GCNGC	4 cut(s)	98, 1288, 1302, 1559
FokI	GGATG	8 cut(s)	47, 98, 829, 1251, 1268, 1293, 1583, 1620
FriOI	GRGCYC	2 cut(s)	1326, 1441
Fsp4HI	GCNGC	4 cut(s)	98, 1288, 1302, 1559
FspBI	CTAG	3 cut(s)	545, 569, 1800
FspI	TGCGCA	1 cut(s)	344
GlaI	GCGC	2 cut(s)	344, 1812
GluI	GCNGC	4 cut(s)	98, 1288, 1302, 1559
GsaI	CCCAGC	2 cut(s)	146, 1390
GsuI	CTGGAG	2 cut(s)	688, 1619
HaeIII	GGCC	4 cut(s)	445, 693, 1539, 1807
HapII	CCGG	3 cut(s)	762, 943, 1804
HhaI	GCGC	2 cut(s)	345, 1813
Hin1I	GRCGYC	1 cut(s)	1094
Hin1II	CATG	9 cut(s)	670, 753, 833, 883, 1508, 1565, 1615, 1771, 1823
Hin6I	GCGC	2 cut(s)	343, 1811
HinP1I	GCGC	2 cut(s)	343, 1811
HincII	GTYRAC	2 cut(s)	755, 901
HindII	GTYRAC	2 cut(s)	755, 901
HindIII	AAGCTT	1 cut(s)	710
HinfI	GANTC	7 cut(s)	119, 194, 292, 464, 503, 969, 1213
HpaI	GTTAAC	1 cut(s)	901
HpaII	CCGG	3 cut(s)	762, 943, 1804
HphI	GGTGA	6 cut(s)	88, 301, 399, 776, 1433, 1727
Hpy166II	GTNNAC	5 cut(s)	755, 781, 792, 901, 1845
Hpy188I	TCNGA	6 cut(s)	415, 486, 528, 1191, 1520, 1677
Hpy188III	TCNNGA	8 cut(s)	368, 545, 584, 636, 842, 1205, 1310, 1354
Hpy8I	GTNNAC	5 cut(s)	755, 781, 792, 901, 1845
Hpy99I	CGWCG	2 cut(s)	1584, 1744
HpyAV	CCTTC	6 cut(s)	1120, 1125, 1170, 1529, 1597, 1672
HpyCH4III	ACNGT	9 cut(s)	236, 836, 905, 1038, 1445, 1498, 1714, 1765, 1787
HpyCH4IV	ACGT	6 cut(s)	757, 992, 1094, 1167, 1582, 1889
HpyF10VI	GCNNNNNNNGC	4 cut(s)	380, 401, 623, 670
HpyF3I	CTNAG	6 cut(s)	127, 222, 398, 483, 1076, 1352
HpySE526I	ACGT	6 cut(s)	757, 992, 1094, 1167, 1582, 1889
Hsp92I	GRCGYC	1 cut(s)	1094
Hsp92II	CATG	9 cut(s)	670, 753, 833, 883, 1508, 1565, 1615, 1771, 1823
HspAI	GCGC	2 cut(s)	343, 1811
KroI	GCCGGC	1 cut(s)	1803
KroNI	GCCGGC	1 cut(s)	1805
Ksp22I	TGATCA	1 cut(s)	1515
KspAI	GTTAAC	1 cut(s)	901
Kzo9I	GATC	4 cut(s)	472, 1149, 1515, 1672
LmnI	GCTCC	2 cut(s)	670, 1321
Lsp1109I	GCAGC	4 cut(s)	109, 1299, 1313, 1545
LweI	GCATC	9 cut(s)	609, 807, 1005, 1144, 1186, 1222, 1561, 1598, 1843
MaeI	CTAG	3 cut(s)	545, 569, 1800
MaeII	ACGT	6 cut(s)	757, 992, 1094, 1167, 1582, 1889
MaeIII	GTNAC	8 cut(s)	120, 236, 289, 727, 1258, 1621, 1733, 1883
MalI	GATC	4 cut(s)	474, 1151, 1517, 1674
MboI	GATC	4 cut(s)	472, 1149, 1515, 1672
MboII	GAAGA	9 cut(s)	240, 243, 246, 361, 560, 989, 1124, 1130, 1460
MfeI	CAATTG	2 cut(s)	681, 1296
MhlI	GDGCHC	2 cut(s)	1326, 1441
MlsI	TGGCCA	1 cut(s)	693
MluNI	TGGCCA	1 cut(s)	693
MlyI	GAGTC	3 cut(s)	128, 286, 473
MmeI	TCCRAC	2 cut(s)	252, 927
Mox20I	TGGCCA	1 cut(s)	693
Mph1103I	ATGCAT	1 cut(s)	1613
MroNI	GCCGGC	1 cut(s)	1803
MroXI	GAANNNNTTC	3 cut(s)	643, 1426, 1841
MscI	TGGCCA	1 cut(s)	693
MseI	TTAA	7 cut(s)	47, 93, 285, 714, 900, 960, 1868
MslI	CAYNNNNRTG	3 cut(s)	834, 1017, 1818
Msp20I	TGGCCA	1 cut(s)	693
MspI	CCGG	3 cut(s)	762, 943, 1804
MspR9I	CCNGG	6 cut(s)	706, 762, 943, 1275, 1375, 1707
MunI	CAATTG	2 cut(s)	681, 1296
MvaI	CCWGG	4 cut(s)	706, 1275, 1375, 1707
MwoI	GCNNNNNNNGC	4 cut(s)	380, 401, 623, 670
NaeI	GCCGGC	1 cut(s)	1805
NciI	CCSGG	2 cut(s)	762, 943
NcoI	CCATGG	1 cut(s)	666
NdeII	GATC	4 cut(s)	472, 1149, 1515, 1672
NgoMIV	GCCGGC	1 cut(s)	1803
NlaIII	CATG	9 cut(s)	670, 753, 833, 883, 1508, 1565, 1615, 1771, 1823
NlaIV	GGNNCC	3 cut(s)	1026, 1323, 1379
NmeAIII	GCCGAG	1 cut(s)	1636
NmuCI	GTSAC	4 cut(s)	120, 236, 289, 1733
NsbI	TGCGCA	1 cut(s)	344
NsiI	ATGCAT	1 cut(s)	1613
NspI	RCATGY	2 cut(s)	753, 1615
PaeI	GCATGC	1 cut(s)	1615
PaeR7I	CTCGAG	1 cut(s)	1368
PciI	ACATGT	1 cut(s)	749
PcsI	WCGNNNNNNNCGW	1 cut(s)	1441
PdiI	GCCGGC	1 cut(s)	1805
PdmI	GAANNNNTTC	3 cut(s)	643, 1426, 1841
PfeI	GAWTC	4 cut(s)	194, 503, 969, 1213
PfoI	TCCNGGA	1 cut(s)	704
PkrI	GCNGC	4 cut(s)	99, 1289, 1303, 1560
PleI	GAGTC	3 cut(s)	127, 286, 472
PpsI	GAGTC	3 cut(s)	127, 286, 472
Ppu21I	YACGTR	2 cut(s)	993, 1890
PscI	ACATGT	1 cut(s)	749
PshAI	GACNNNNGTC	1 cut(s)	837
Psp124BI	GAGCTC	1 cut(s)	1441
Psp6I	CCWGG	4 cut(s)	704, 1273, 1373, 1705
PspFI	CCCAGC	2 cut(s)	142, 1386
PspGI	CCWGG	4 cut(s)	704, 1273, 1373, 1705
PspN4I	GGNNCC	3 cut(s)	1026, 1323, 1379
PspPI	GGNCC	4 cut(s)	179, 443, 949, 1378
PspXI	VCTCGAGB	1 cut(s)	1368
PsrI	GAACNNNNNNTAC	4 cut(s)	1017, 1049, 1397, 1429
PstNI	CAGNNNCTG	2 cut(s)	1712, 1785
RsaI	GTAC	8 cut(s)	113, 510, 593, 679, 1035, 1415, 1784, 1793
RsaNI	GTAC	8 cut(s)	112, 509, 592, 678, 1034, 1414, 1783, 1792
RseI	CAYNNNNRTG	3 cut(s)	834, 1017, 1818
SacI	GAGCTC	1 cut(s)	1441
SaqAI	TTAA	7 cut(s)	47, 93, 285, 714, 900, 960, 1868
SatI	GCNGC	4 cut(s)	98, 1288, 1302, 1559
Sau3AI	GATC	4 cut(s)	472, 1149, 1515, 1672
Sau96I	GGNCC	4 cut(s)	179, 443, 949, 1378
ScaI	AGTACT	1 cut(s)	510
SchI	GAGTC	3 cut(s)	128, 286, 473
ScrFI	CCNGG	6 cut(s)	706, 762, 943, 1275, 1375, 1707
SduI	GDGCHC	2 cut(s)	1326, 1441
SfaNI	GCATC	9 cut(s)	609, 807, 1005, 1144, 1186, 1222, 1561, 1598, 1843
Sfr274I	CTCGAG	1 cut(s)	1368
SinI	GGWCC	3 cut(s)	179, 949, 1378
SlaI	CTCGAG	1 cut(s)	1368
SmiMI	CAYNNNNRTG	3 cut(s)	834, 1017, 1818
SmlI	CTYRAG	2 cut(s)	366, 1368
SmoI	CTYRAG	2 cut(s)	366, 1368
SpeI	ACTAGT	1 cut(s)	568
SphI	GCATGC	1 cut(s)	1615
SsiI	CCGC	2 cut(s)	1173, 1253
SspI	AATATT	4 cut(s)	9, 532, 578, 1699
SspMI	CTAG	3 cut(s)	545, 569, 1800
SstI	GAGCTC	1 cut(s)	1441
StyD4I	CCNGG	6 cut(s)	704, 760, 941, 1273, 1373, 1705
StyI	CCWWGG	1 cut(s)	666
TaaI	ACNGT	9 cut(s)	236, 836, 905, 1038, 1445, 1498, 1714, 1765, 1787
TaiI	ACGT	6 cut(s)	760, 995, 1097, 1170, 1585, 1892
TaqI	TCGA	4 cut(s)	263, 863, 1369, 1830
TatI	WGTACW	3 cut(s)	111, 508, 677
TfiI	GAWTC	4 cut(s)	194, 503, 969, 1213
Tru1I	TTAA	7 cut(s)	47, 93, 285, 714, 900, 960, 1868
Tru9I	TTAA	7 cut(s)	47, 93, 285, 714, 900, 960, 1868
TscAI	CASTG	4 cut(s)	72, 841, 1717, 1852
TseFI	GTSAC	4 cut(s)	120, 236, 289, 1733
TseI	GCWGC	4 cut(s)	97, 1287, 1301, 1558
Tsp45I	GTSAC	4 cut(s)	120, 236, 289, 1733
TspDTI	ATGAA	5 cut(s)	173, 732, 1076, 1493, 1578
TspGWI	ACGGA	4 cut(s)	567, 1071, 1757, 1809
TspRI	CASTG	4 cut(s)	72, 841, 1717, 1852
VpaK11BI	GGWCC	3 cut(s)	179, 949, 1378
XapI	RAATTY	5 cut(s)	203, 281, 955, 1393, 1775
XbaI	TCTAGA	1 cut(s)	544
XceI	RCATGY	2 cut(s)	753, 1615
XhoI	CTCGAG	1 cut(s)	1368
XmiI	GTMKAC	2 cut(s)	780, 791
XmnI	GAANNNNTTC	3 cut(s)	643, 1426, 1841
XspI	CTAG	3 cut(s)	545, 569, 1800
ZraI	GACGTC	1 cut(s)	1095
ZrmI	AGTACT	1 cut(s)	510
Zsp2I	ATGCAT	1 cut(s)	1613

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.