Rw0G004500

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00168
OG0001300
Physical Location & Seq
Forward (+)
257124 .. 264638
7515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004500.1

Sequence Viewer

Length: 2931 bp
ATGACCGCCGCCGGACAGGGAGCTATCTGGTGGAGAGCCAACACCGCAATGTCCAATCGCGGAGGTTCATCCGGTAGTCGCGGCGGCGGAGGCCGCGGCCGCGGCCGCGGAAGAGGAAATCCCGGCGGTCAGGCCAGAGGCTCAGGCTACACTCCGGCGCCCGCGCCTGCTCCGGCGCCTGCTCCAGCTCCAGCTCCTGCTCCGGCGTATGCTCCGGCGCCGGCTCGTGCTCAGCCGCCTGCTGTGGCCTCGTCGAGCAGGGCTCCGGCTCCGGCTCCAGCTCCTGCTCCGGCTCCGGCGCCGGCACCTGTTTCATCGGCGGCGGCGGCGGCGGCTTCGCTGGCGAGTGAGATGGAAGAGAAGCTTGCATTGGCTCCGTCGTCATCCAAGGCCGTGAGGTTTCCAGTGAGGCCGGGGTTTGGTAGAGCTGGTAAAAGAGTCCAAGTCCGAGCCAATCATTTTCTGGTCGAGATTGGGGAAAGAGATCTGCATCACTATGATGTATCAATTAGCCCTGAAGTTGCTTCAAAGAAGATAAATAGAGATGTAATCAACCAGCTGGTTCAGATGTACTCTGAGTCTCACTTGGGAAAGAGAATGCCAGCATATGATGGCATGAAGAGTATCTACACTGCCGGGCCATTGCCCTTCTCTTCAAAGGAGTTTGTGGTCAAGCTACCTGAGAGAGAAGGTCGAGCTGGATCTGCATCAAGAAGGAAAGATAGAGACTTCAAGGTCTGCATTAAGTTGGCAAACAAGCCTGACCTGTATCAACTGCAGCAGTTCTTACGCGGAGGACTACACGAAACACCACAGGAGACCATCCAAGTTCTTGATGTTGTTCTTAGAGCTGCACCATCACAGAAATACTGTGTGGTTGGTAGGTCATTTTTTAGCCCTGATCTGGGTCCTCGAGGTGACCTTGAATTTGGCTTGGAATACTGGAGAGGCTTTTACCAAAGCCTAAGGCCAACTCAGTTTGGTCTATCCCTAAATATTGATATATCAGCAAGGGCTTTTTATGATCCGATTCTGGTGACAGAGTTTATTAAGAAACATTTTGGGTACAGAGATTTATCCAGGGGTCTGTCGGATCGTGATTGTCTGAAGCTGAAGAAAAATCTGAAAGGAATCAAGGTAACTCTGAGCTATGGAGGTAGCAGAAGTTATCGAATTACTGGAGTATCTGCGCAGCCACTAAGCCAGTTAACGTTTACTCTTGAGGATAATCAAACCAGAGTATCAGTGGTTCAGTATTATCGTGAGAAGTACAACATTAGCCTAAAACAGGTGGCTTTGCCTGCCCTTCAAGCTGGCAATGATTCAAGGCCTGCTTATTTGCCCATGGAGCTTGCTTCAATTGTTCCCGGGCAGAGATACACGAAGAGGCTCAATGAAAAGCAAGTGACAAACGTGCTGAGAGCAACTTGCCAACGGCCCCGGGAGAGGGAATCCAATATACTGAAGATGGTGATGCACAATAATTACAAGGGAGAGGATCTTGTAAACAAAGAATTTGGAATACAAGTGAAAGAACAAATGGCACTGGTGGATGCTAGAATATTACCACCTCCAATGCTTAAGTATCATGAGAGTGGTCGTGAAACGAAGGATACTCCCAGGATGGGAGCATGGAACATGATCAATAAGAAAATGGTTAATGGTGCCAAAGTGGATTTTTGGGCTTTTGTCAATTTCTCTCGATCACGTGAAGATTTTAACTATCGCTTTTGTGAGGAACTCGTCTATATGTGCAACAGTAAAGGGGTGGAGTTCCATTCCGAGCCTTTACTTCCTGTACAGTCAGCTCATCCTAATCAAATTGAGAGGGTTCTGAGAGATATTCACAGGCAGTCAGTCCAGAGACTCAAGGATATGGCACAAGCCAGCAAACATCTTCAGTTGTTGATTATCATCTTGCCTGATGTCAAAGGATCTTATGGTCCCATTAAAAGAATTTGTGAAACAGAGCTTGGAATTGTCTCCCAGTGCTGTAATCCTAAGCAAGCACAAAAGCTTAGTAAGCAATACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGAGGAGGATTCCTCTTGTCAGTGATCGTCCTACAATCATCCTTGGAGCTGATGTCACTCATCCACAAGCTGGGGAAGACAGTAGCCCATCTATAGCAGCAGTGGTTGCCTCAATGGACTGGCCAGAGGTCTCCAAGTATAGAGGAATCCTGTCAGCACAGAATCATCGTCAAGAAATCATTCAAGATCTTTACAAATTAACTCACGATGAGAAAAGAGGGCCGGTTCATTCAGGAATGATTAGAGAACTTTTCATTGCATTCCGAAGATCGACTGGCCAAAAGCCGCACAGAATCATCTTCTATAGGGATGGGGTGAGTGAAGGGCAGTTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACGGAAGGCCTGTGCTTCTTTGGAGGAGGGGTATATGCCACCAGTTACTTTTGTTGTGGTGCAGAAAAGGCATCACACACGCCTTTTCCCTGCTGACAACCAGACAGATAGGAGTGGGAATATTCAACCAGGTACTGTGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTATCTTAATAGCCATGCAGGAATTCAGGGAACAAGTCGCCCAGCTCATTATCATGTCCTGTTTGATGAGAACAACTTCACTGCTGATGGCTTGCAGATTCTCACCAACAATCTGTGCTACACATATGCAAGGTGCACCCGCTCAGTTTCCATAGTGCCTCCTGCCTATTATGCGCATCTAGCTGCGTTTAGAGCACGCTATTACATTGAGGGCGATTCATCTGATGGTGCTTCTTCAGTTGGCAGAGGCAGCGGTGGCAGTGCTGCACAAACCCAGGTCCTTCCAAATGTGCACGAGTATGTCAAGGATGTGATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

976

Amino Acids

108.32

Weight (kDa)

9.59

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 145 - 282 2.6e-37 N-terminal domain of argonaute
ArgoL1 PF08699 293 - 341 3.4e-18 Argonaute linker 1 domain
PAZ PF02170 369 - 472 5.6e-27 PAZ domain
ArgoL2 PF16488 482 - 529 3.1e-13 Argonaute linker 2 domain
ArgoMid PF16487 539 - 617 3.1e-09 Mid domain of argonaute
Piwi PF02171 636 - 937 5e-101 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
AbsI CCTCGAGG 1 cut(s) 912
Acc16I TGCGCA 2 cut(s) 1191, 2778
AccB1I GGYRCC 6 cut(s) 157, 175, 217, 298, 304, 1664
AccB7I CCANNNNNTGG 1 cut(s) 2132
AccBSI CCGCTC 1 cut(s) 2745
AccII CGCG 7 cut(s) 60, 81, 96, 102, 108, 164, 792
AclI AACGTT 1 cut(s) 1211
AclWI GGATC 5 cut(s) 709, 1019, 1101, 1506, 1942
AcoI YGGCCR 4 cut(s) 97, 103, 2183, 2338
AcsI RAATTY 5 cut(s) 926, 1514, 1956, 2594, 2625
AcuI CTGAAG 6 cut(s) 537, 1127, 1133, 1484, 1883, 2823
AcvI CACGTG 1 cut(s) 1709
AcyI GRCGYC 4 cut(s) 158, 176, 218, 299
AfaI GTAC 5 cut(s) 572, 1067, 1271, 1800, 2560
AfiI CCNNNNNNNGG 8 cut(s) 419, 904, 905, 1288, 1446, 1447, 1625, 2132
AflII CTTAAG 2 cut(s) 1580, 2048
AjnI CCWGG 4 cut(s) 1079, 1619, 2554, 2877
AjuI GAANNNNNNNTTGG 4 cut(s) 353, 385, 1956, 1988
Alw21I GWGCWC 4 cut(s) 232, 2741, 2800, 2898
Alw26I GTCTC 7 cut(s) 585, 720, 812, 1858, 1987, 2049, 2197
Alw44I GTGCAC 2 cut(s) 2737, 2894
AlwI GGATC 5 cut(s) 709, 1019, 1101, 1506, 1942
AlwNI CAGNNNCTG 3 cut(s) 197, 284, 2561
Ama87I CYCGRG 3 cut(s) 912, 1367, 1440
ApaLI GTGCAC 2 cut(s) 2737, 2894
ApeKI GCWGC 7 cut(s) 778, 851, 1192, 2159, 2786, 2853, 2867
ApoI RAATTY 5 cut(s) 926, 1514, 1956, 2594, 2625
ArsI GACNNNNNNTTYG 2 cut(s) 911, 943
AseI ATTAAT 1 cut(s) 2055
Asp700I GAANNNNTTC 2 cut(s) 2241, 2678
AspLEI GCGC 7 cut(s) 160, 166, 178, 220, 301, 1192, 2779
AspS9I GGNCC 6 cut(s) 638, 908, 1437, 1943, 2282, 2881
AsuC2I CCSGG 7 cut(s) 123, 414, 637, 1368, 1369, 1441, 1442
AsuHPI GGTGA 5 cut(s) 929, 1048, 1483, 2389, 2698
AvaI CYCGRG 3 cut(s) 912, 1367, 1440
AvaII GGWCC 3 cut(s) 908, 1943, 2881
AxyI CCTNAGG 1 cut(s) 965
BaeGI GKGCMC 2 cut(s) 2741, 2898
BaeI ACNNNNGTAYC 6 cut(s) 1167, 1200, 1224, 1257, 2550, 2583
BalI TGGCCA 2 cut(s) 2185, 2340
BanI GGYRCC 6 cut(s) 157, 175, 217, 298, 304, 1664
BanII GRGCYC 1 cut(s) 265
BarI GAAGNNNNNNTAC 2 cut(s) 611, 643
BauI CACGAG 2 cut(s) 225, 2897
BbrPI CACGTG 1 cut(s) 1709
BbsI GAAGAC 1 cut(s) 2145
Bbv12I GWGCWC 4 cut(s) 232, 2741, 2800, 2898
BbvI GCAGC 7 cut(s) 790, 838, 1204, 2171, 2773, 2854, 2865
BceAI ACGGC 2 cut(s) 377, 1451
BciT130I CCWGG 4 cut(s) 1081, 1621, 2556, 2879
BciVI GTATCC 2 cut(s) 1606, 2563
BclI TGATCA 1 cut(s) 1641
BcnI CCSGG 7 cut(s) 123, 414, 637, 1368, 1369, 1441, 1442
BcoDI GTCTC 7 cut(s) 585, 720, 812, 1858, 1987, 2049, 2197
BfaI CTAG 2 cut(s) 1557, 2783
BfmI CTRYAG 3 cut(s) 776, 2154, 2365
BfoI RGCGCY 4 cut(s) 161, 179, 221, 302
BfrI CTTAAG 2 cut(s) 1580, 2048
BfuI GTATCC 2 cut(s) 1606, 2563
BglII AGATCT 2 cut(s) 484, 2248
BlpI GCTNAGC 1 cut(s) 231
Bme18I GGWCC 3 cut(s) 908, 1943, 2881
BmeT110I CYCGRG 3 cut(s) 912, 1367, 1440
BmgT120I GGNCC 6 cut(s) 638, 908, 1437, 1943, 2282, 2881
BmrI ACTGGG 1 cut(s) 1981
BmsI GCATC 7 cut(s) 499, 716, 1464, 1543, 2410, 2506, 2788
BmuI ACTGGG 1 cut(s) 1981
BpiI GAAGAC 1 cut(s) 2145
BplI GAGNNNNNCTC 4 cut(s) 247, 279, 2059, 2091
BpmI CTGGAG 5 cut(s) 168, 174, 261, 964, 1200
Bpu10I CCTNAGC 2 cut(s) 142, 2001
Bpu1102I GCTNAGC 1 cut(s) 231
BpuEI CTTGAG 2 cut(s) 1241, 1853
BpuMI CCSGG 7 cut(s) 123, 414, 637, 1368, 1369, 1441, 1442
BsaAI YACGTR 1 cut(s) 1709
BsaBI GATNNNNATC 4 cut(s) 489, 706, 1913, 2604
BsaHI GRCGYC 4 cut(s) 158, 176, 218, 299
BsaI GGTCTC 2 cut(s) 812, 2197
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 4 cut(s) 2100, 2130, 2441, 2471
Bsc4I CCNNNNNNNGG 8 cut(s) 419, 904, 905, 1288, 1446, 1447, 1625, 2132
Bse118I RCCGGY 3 cut(s) 220, 301, 2284
Bse1I ACTGG 9 cut(s) 404, 947, 1183, 1204, 1551, 1987, 2186, 2341, 2468
Bse21I CCTNAGG 1 cut(s) 965
Bse3DI GCAATG 4 cut(s) 54, 641, 1324, 2316
Bse8I GATNNNNATC 4 cut(s) 489, 706, 1913, 2604
BseBI CCWGG 4 cut(s) 1081, 1621, 2556, 2879
BseJI GATNNNNATC 4 cut(s) 489, 706, 1913, 2604
BseLI CCNNNNNNNGG 8 cut(s) 419, 904, 905, 1288, 1446, 1447, 1625, 2132
BseMI GCAATG 4 cut(s) 54, 641, 1324, 2316
BseMII CTCAG 9 cut(s) 156, 245, 567, 672, 989, 1136, 1409, 1826, 2760
BseNI ACTGG 9 cut(s) 404, 947, 1183, 1204, 1551, 1987, 2186, 2341, 2468
BseRI GAGGAG 2 cut(s) 2080, 2465
BseSI GKGCMC 2 cut(s) 2741, 2898
BseX3I CGGCCG 2 cut(s) 97, 103
BseXI GCAGC 7 cut(s) 790, 838, 1204, 2171, 2773, 2854, 2865
BseYI CCCAGC 2 cut(s) 2132, 2644
BsgI GTGCAG 3 cut(s) 837, 2507, 2853
Bsh1236I CGCG 7 cut(s) 60, 81, 96, 102, 108, 164, 792
Bsh1285I CGRYCG 2 cut(s) 100, 106
BshNI GGYRCC 6 cut(s) 157, 175, 217, 298, 304, 1664
BsiEI CGRYCG 2 cut(s) 100, 106
BsiHKAI GWGCWC 4 cut(s) 232, 2741, 2800, 2898
BsiHKCI CYCGRG 3 cut(s) 912, 1367, 1440
BslFI GGGAC 1 cut(s) 1929
BslI CCNNNNNNNGG 8 cut(s) 419, 904, 905, 1288, 1446, 1447, 1625, 2132
BsmAI GTCTC 7 cut(s) 585, 720, 812, 1858, 1987, 2049, 2197
BsmFI GGGAC 1 cut(s) 1929
BsmI GAATGC 2 cut(s) 603, 2321
Bso31I GGTCTC 2 cut(s) 812, 2197
BsoBI CYCGRG 3 cut(s) 912, 1367, 1440
Bsp1286I GDGCHC 5 cut(s) 232, 265, 2741, 2800, 2898
Bsp1407I TGTACA 1 cut(s) 1798
Bsp1720I GCTNAGC 1 cut(s) 231
Bsp19I CCATGG 1 cut(s) 1344
BspCNI CTCAG 9 cut(s) 155, 244, 568, 673, 988, 1137, 1410, 1827, 2759
BspFNI CGCG 7 cut(s) 60, 81, 96, 102, 108, 164, 792
BspHI TCATGA 1 cut(s) 1588
BspMAI CTGCAG 1 cut(s) 780
BspPI GGATC 5 cut(s) 709, 1019, 1101, 1506, 1942
BspT107I GGYRCC 6 cut(s) 157, 175, 217, 298, 304, 1664
BspTI CTTAAG 2 cut(s) 1580, 2048
BspTNI GGTCTC 2 cut(s) 812, 2197
BsrBI CCGCTC 1 cut(s) 2745
BsrDI GCAATG 4 cut(s) 54, 641, 1324, 2316
BsrFI RCCGGY 3 cut(s) 220, 301, 2284
BsrGI TGTACA 1 cut(s) 1798
BsrI ACTGG 9 cut(s) 404, 947, 1183, 1204, 1551, 1987, 2186, 2341, 2468
BssAI RCCGGY 3 cut(s) 220, 301, 2284
BssNI GRCGYC 4 cut(s) 158, 176, 218, 299
BssSI CACGAG 2 cut(s) 225, 2897
BssT1I CCWWGG 3 cut(s) 387, 1344, 2104
Bst2BI CACGAG 2 cut(s) 225, 2897
Bst2UI CCWGG 4 cut(s) 1081, 1621, 2556, 2879
Bst4CI ACNGT 5 cut(s) 872, 1760, 1803, 2144, 2563
Bst6I CTCTTC 5 cut(s) 106, 351, 614, 658, 1379
BstACI GRCGYC 4 cut(s) 158, 176, 218, 299
BstAFI CTTAAG 2 cut(s) 1580, 2048
BstAPI GCANNNNNTGC 1 cut(s) 2168
BstAUI TGTACA 1 cut(s) 1798
BstBAI YACGTR 1 cut(s) 1709
BstDSI CCRYGG 4 cut(s) 94, 100, 106, 1344
BstEII GGTNACC 1 cut(s) 917
BstENI CCTNNNNNAGG 1 cut(s) 1286
BstFNI CGCG 7 cut(s) 60, 81, 96, 102, 108, 164, 792
BstH2I RGCGCY 4 cut(s) 161, 179, 221, 302
BstHHI GCGC 7 cut(s) 160, 166, 178, 220, 301, 1192, 2779
BstMAI GTCTC 7 cut(s) 585, 720, 812, 1858, 1987, 2049, 2197
BstMCI CGRYCG 2 cut(s) 100, 106
BstNI CCWGG 4 cut(s) 1081, 1621, 2556, 2879
BstPI GGTNACC 1 cut(s) 917
BstSFI CTRYAG 3 cut(s) 776, 2154, 2365
BstSLI GKGCMC 2 cut(s) 2741, 2898
BstUI CGCG 7 cut(s) 60, 81, 96, 102, 108, 164, 792
BstV1I GCAGC 7 cut(s) 790, 838, 1204, 2171, 2773, 2854, 2865
BstV2I GAAGAC 1 cut(s) 2145
BstX2I RGATCY 5 cut(s) 484, 701, 1498, 1934, 2248
BstYI RGATCY 5 cut(s) 484, 701, 1498, 1934, 2248
BstZI CGGCCG 2 cut(s) 97, 103
Bsu36I CCTNAGG 1 cut(s) 965
BsuI GTATCC 2 cut(s) 1606, 2563
BtgI CCRYGG 4 cut(s) 94, 100, 106, 1344
BtsI GCAGTG 4 cut(s) 630, 2169, 2682, 2869
BtsIMutI CAGTG 9 cut(s) 411, 630, 1251, 1544, 1994, 2089, 2169, 2682, 2869
CaiI CAGNNNCTG 3 cut(s) 197, 284, 2561
CciI TCATGA 1 cut(s) 1588
CciNI GCGGCCGC 2 cut(s) 97, 103
CfoI GCGC 7 cut(s) 160, 166, 178, 220, 301, 1192, 2779
Cfr10I RCCGGY 3 cut(s) 220, 301, 2284
Cfr13I GGNCC 6 cut(s) 638, 908, 1437, 1943, 2282, 2881
Cfr42I CCGCGG 3 cut(s) 97, 103, 109
Cfr9I CCCGGG 2 cut(s) 1367, 1440
CsiI ACCWGGT 1 cut(s) 2554
Csp6I GTAC 5 cut(s) 571, 1066, 1270, 1799, 2559
CviAII CATG 7 cut(s) 616, 1345, 1589, 1632, 1639, 2618, 2657
CviQI GTAC 5 cut(s) 571, 1066, 1270, 1799, 2559
DinI GGCGCC 4 cut(s) 159, 177, 219, 300
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 4 cut(s) 97, 103, 2183, 2338
EagI CGGCCG 2 cut(s) 97, 103
Eam1104I CTCTTC 5 cut(s) 106, 351, 614, 658, 1379
EarI CTCTTC 5 cut(s) 106, 351, 614, 658, 1379
EciI GGCGGA 1 cut(s) 102
EclXI CGGCCG 2 cut(s) 97, 103
Eco130I CCWWGG 3 cut(s) 387, 1344, 2104
Eco147I AGGCCT 2 cut(s) 1330, 2435
Eco24I GRGCYC 1 cut(s) 265
Eco31I GGTCTC 2 cut(s) 812, 2197
Eco47I GGWCC 3 cut(s) 908, 1943, 2881
Eco52I CGGCCG 2 cut(s) 97, 103
Eco57I CTGAAG 6 cut(s) 537, 1127, 1133, 1484, 1883, 2823
Eco72I CACGTG 1 cut(s) 1709
Eco81I CCTNAGG 1 cut(s) 965
Eco88I CYCGRG 3 cut(s) 912, 1367, 1440
Eco91I GGTNACC 1 cut(s) 917
EcoNI CCTNNNNNAGG 1 cut(s) 1286
EcoO109I RGGNCCY 2 cut(s) 908, 2881
EcoO65I GGTNACC 1 cut(s) 917
EcoRI GAATTC 1 cut(s) 2625
EcoRII CCWGG 4 cut(s) 1079, 1619, 2554, 2877
EcoT14I CCWWGG 3 cut(s) 387, 1344, 2104
EcoT38I GRGCYC 1 cut(s) 265
EgeI GGCGCC 4 cut(s) 159, 177, 219, 300
EheI GGCGCC 4 cut(s) 159, 177, 219, 300
ErhI CCWWGG 3 cut(s) 387, 1344, 2104
FaeI CATG 7 cut(s) 619, 1348, 1592, 1635, 1642, 2621, 2660
FalI AAGNNNNNCTT 4 cut(s) 348, 380, 1318, 1350
FaqI GGGAC 1 cut(s) 1929
FatI CATG 7 cut(s) 615, 1344, 1588, 1631, 1638, 2617, 2656
FauI CCCGC 2 cut(s) 169, 2750
FauNDI CATATG 2 cut(s) 607, 2728
FbaI TGATCA 1 cut(s) 1641
FriOI GRGCYC 1 cut(s) 265
FspAI RTGCGCAY 1 cut(s) 2778
FspBI CTAG 2 cut(s) 1557, 2783
FspI TGCGCA 2 cut(s) 1191, 2778
GlaI GCGC 7 cut(s) 159, 165, 177, 219, 300, 1191, 2778
GsaI CCCAGC 2 cut(s) 2136, 2648
GsuI CTGGAG 5 cut(s) 168, 174, 261, 964, 1200
HaeII RGCGCY 4 cut(s) 161, 179, 221, 302
HhaI GCGC 7 cut(s) 160, 166, 178, 220, 301, 1192, 2779
Hin1I GRCGYC 4 cut(s) 158, 176, 218, 299
Hin1II CATG 7 cut(s) 619, 1348, 1592, 1635, 1642, 2621, 2660
Hin6I GCGC 7 cut(s) 158, 164, 176, 218, 299, 1190, 2777
HinP1I GCGC 7 cut(s) 158, 164, 176, 218, 299, 1190, 2777
HincII GTYRAC 1 cut(s) 1209
HindII GTYRAC 1 cut(s) 1209
HindIII AAGCTT 2 cut(s) 362, 2015
HpaI GTTAAC 1 cut(s) 1209
HphI GGTGA 5 cut(s) 929, 1048, 1483, 2389, 2698
Hpy166II GTNNAC 5 cut(s) 1209, 1215, 1507, 2739, 2896
Hpy8I GTNNAC 5 cut(s) 1209, 1215, 1507, 2739, 2896
Hpy99I CGWCG 2 cut(s) 256, 382
HpyAV CCTTC 8 cut(s) 658, 683, 708, 1316, 1603, 2378, 2425, 2894
HpyCH4III ACNGT 5 cut(s) 872, 1760, 1803, 2144, 2563
HpyCH4IV ACGT 3 cut(s) 1211, 1413, 1708
HpySE526I ACGT 3 cut(s) 1211, 1413, 1708
Hsp92I GRCGYC 4 cut(s) 158, 176, 218, 299
Hsp92II CATG 7 cut(s) 619, 1348, 1592, 1635, 1642, 2621, 2660
HspAI GCGC 7 cut(s) 158, 164, 176, 218, 299, 1190, 2777
KasI GGCGCC 4 cut(s) 157, 175, 217, 298
KroI GCCGGC 2 cut(s) 220, 301
KroNI GCCGGC 2 cut(s) 222, 303
Ksp22I TGATCA 1 cut(s) 1641
KspAI GTTAAC 1 cut(s) 1209
KspI CCGCGG 3 cut(s) 97, 103, 109
Lsp1109I GCAGC 7 cut(s) 790, 838, 1204, 2171, 2773, 2854, 2865
LweI GCATC 7 cut(s) 499, 716, 1464, 1543, 2410, 2506, 2788
MabI ACCWGGT 1 cut(s) 2554
MaeI CTAG 2 cut(s) 1557, 2783
MaeII ACGT 3 cut(s) 1211, 1413, 1708
MaeIII GTNAC 6 cut(s) 917, 1036, 1138, 1405, 2116, 2470
MbiI CCGCTC 1 cut(s) 2745
MfeI CAATTG 1 cut(s) 1359
MflI RGATCY 5 cut(s) 484, 701, 1498, 1934, 2248
MhlI GDGCHC 5 cut(s) 232, 265, 2741, 2800, 2898
MlsI TGGCCA 2 cut(s) 2185, 2340
MluNI TGGCCA 2 cut(s) 2185, 2340
Mly113I GGCGCC 4 cut(s) 158, 176, 218, 299
MlyI GAGTC 3 cut(s) 447, 587, 1860
MmeI TCCRAC 2 cut(s) 1071, 2612
Mox20I TGGCCA 2 cut(s) 2185, 2340
MroNI GCCGGC 2 cut(s) 220, 301
MroXI GAANNNNTTC 2 cut(s) 2241, 2678
MscI TGGCCA 2 cut(s) 2185, 2340
MslI CAYNNNNRTG 6 cut(s) 47, 495, 498, 1593, 2655, 2901
Msp20I TGGCCA 2 cut(s) 2185, 2340
MspA1I CMGCKG 5 cut(s) 96, 102, 108, 559, 2856
MspCI CTTAAG 2 cut(s) 1580, 2048
MunI CAATTG 1 cut(s) 1359
Mva1269I GAATGC 2 cut(s) 603, 2321
MvaI CCWGG 4 cut(s) 1081, 1621, 2556, 2879
MvnI CGCG 7 cut(s) 60, 81, 96, 102, 108, 164, 792
NaeI GCCGGC 2 cut(s) 222, 303
NarI GGCGCC 4 cut(s) 158, 176, 218, 299
NciI CCSGG 7 cut(s) 123, 414, 637, 1368, 1369, 1441, 1442
NcoI CCATGG 1 cut(s) 1344
NdeI CATATG 2 cut(s) 607, 2728
NgoMIV GCCGGC 2 cut(s) 220, 301
NlaIII CATG 7 cut(s) 619, 1348, 1592, 1635, 1642, 2621, 2660
NmuCI GTSAC 4 cut(s) 917, 1036, 1405, 2116
NotI GCGGCCGC 2 cut(s) 97, 103
NsbI TGCGCA 2 cut(s) 1191, 2778
PaeR7I CTCGAG 1 cut(s) 912
PagI TCATGA 1 cut(s) 1588
PceI AGGCCT 2 cut(s) 1330, 2435
PctI GAATGC 2 cut(s) 603, 2321
PdiI GCCGGC 2 cut(s) 222, 303
PdmI GAANNNNTTC 2 cut(s) 2241, 2678
PflMI CCANNNNNTGG 1 cut(s) 2132
PleI GAGTC 3 cut(s) 446, 586, 1860
PluTI GGCGCC 4 cut(s) 161, 179, 221, 302
PmaCI CACGTG 1 cut(s) 1709
PmlI CACGTG 1 cut(s) 1709
PpsI GAGTC 3 cut(s) 446, 586, 1860
Ppu21I YACGTR 1 cut(s) 1709
PpuMI RGGWCCY 2 cut(s) 908, 2881
PshBI ATTAAT 1 cut(s) 2055
Psp1406I AACGTT 1 cut(s) 1211
Psp5II RGGWCCY 2 cut(s) 908, 2881
Psp6I CCWGG 4 cut(s) 1079, 1619, 2554, 2877
PspCI CACGTG 1 cut(s) 1709
PspEI GGTNACC 1 cut(s) 917
PspFI CCCAGC 2 cut(s) 2132, 2644
PspGI CCWGG 4 cut(s) 1079, 1619, 2554, 2877
PspPI GGNCC 6 cut(s) 638, 908, 1437, 1943, 2282, 2881
PspPPI RGGWCCY 2 cut(s) 908, 2881
PspXI VCTCGAGB 1 cut(s) 912
PsrI GAACNNNNNNTAC 2 cut(s) 1233, 1265
PstI CTGCAG 1 cut(s) 780
PstNI CAGNNNCTG 3 cut(s) 197, 284, 2561
PsuI RGATCY 5 cut(s) 484, 701, 1498, 1934, 2248
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 5 cut(s) 572, 1067, 1271, 1800, 2560
RsaNI GTAC 5 cut(s) 571, 1066, 1270, 1799, 2559
RseI CAYNNNNRTG 6 cut(s) 47, 495, 498, 1593, 2655, 2901
SacII CCGCGG 3 cut(s) 97, 103, 109
Sau96I GGNCC 6 cut(s) 638, 908, 1437, 1943, 2282, 2881
SchI GAGTC 3 cut(s) 447, 587, 1860
SduI GDGCHC 5 cut(s) 232, 265, 2741, 2800, 2898
SexAI ACCWGGT 1 cut(s) 2554
SfaNI GCATC 7 cut(s) 499, 716, 1464, 1543, 2410, 2506, 2788
SfcI CTRYAG 3 cut(s) 776, 2154, 2365
SfoI GGCGCC 4 cut(s) 159, 177, 219, 300
Sfr274I CTCGAG 1 cut(s) 912
Sfr303I CCGCGG 3 cut(s) 97, 103, 109
SgrBI CCGCGG 3 cut(s) 97, 103, 109
SinI GGWCC 3 cut(s) 908, 1943, 2881
SlaI CTCGAG 1 cut(s) 912
SmaI CCCGGG 2 cut(s) 1369, 1442
SmiMI CAYNNNNRTG 6 cut(s) 47, 495, 498, 1593, 2655, 2901
SmlI CTYRAG 5 cut(s) 912, 1220, 1580, 1868, 2048
SmoI CTYRAG 5 cut(s) 912, 1220, 1580, 1868, 2048
SseBI AGGCCT 2 cut(s) 1330, 2435
SspDI GGCGCC 4 cut(s) 157, 175, 217, 298
SspI AATATT 3 cut(s) 997, 1563, 2548
SspMI CTAG 2 cut(s) 1557, 2783
StuI AGGCCT 2 cut(s) 1330, 2435
StyI CCWWGG 3 cut(s) 387, 1344, 2104
TaaI ACNGT 5 cut(s) 872, 1760, 1803, 2144, 2563
TaiI ACGT 3 cut(s) 1214, 1416, 1711
TaqI TCGA 7 cut(s) 254, 468, 694, 913, 1171, 1702, 2333
TatI WGTACW 3 cut(s) 570, 1269, 1798
TscAI CASTG 9 cut(s) 411, 637, 1251, 1551, 1994, 2089, 2169, 2689, 2869
TseFI GTSAC 4 cut(s) 917, 1036, 1405, 2116
TseI GCWGC 7 cut(s) 778, 851, 1192, 2159, 2786, 2853, 2867
Tsp45I GTSAC 4 cut(s) 917, 1036, 1405, 2116
TspDTI ATGAA 7 cut(s) 57, 303, 632, 1410, 2279, 2305, 2811
TspGWI ACGGA 2 cut(s) 366, 2443
TspMI CCCGGG 2 cut(s) 1367, 1440
TspRI CASTG 9 cut(s) 411, 637, 1251, 1551, 1994, 2089, 2169, 2689, 2869
Van91I CCANNNNNTGG 1 cut(s) 2132
Vha464I CTTAAG 2 cut(s) 1580, 2048
VneI GTGCAC 2 cut(s) 2737, 2894
VpaK11BI GGWCC 3 cut(s) 908, 1943, 2881
VspI ATTAAT 1 cut(s) 2055
XagI CCTNNNNNAGG 1 cut(s) 1286
XapI RAATTY 5 cut(s) 926, 1514, 1956, 2594, 2625
XcmI CCANNNNNNNNNTGG 2 cut(s) 460, 1243
XhoI CTCGAG 1 cut(s) 912
XmaI CCCGGG 2 cut(s) 1367, 1440
XmnI GAANNNNTTC 2 cut(s) 2241, 2678
XspI CTAG 2 cut(s) 1557, 2783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.