Rh5DG391700

DUF1785

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001300
Physical Location & Seq
Reverse (-)
61278016 .. 61284893
6878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG391700.1

Sequence Viewer

Length: 2403 bp
ATGTACTCTGAGTCTCACTTGGGAAAGAGAATGCCAGCATATGATGGCATGAAGAGTATCTACACTGCCGGGCCATTGCCCTTCTCTTCAAAGGAGTTTGTGGTCAAGCTACCTGAGAGAGAAGGTCGAGCTGGATCTGCATCAAGAAGGAAAGATAGAGACTTCAAGGTCTGCATTAAGTTGGCAAACAAGCCTGACCTGTATCAACTGCAGCAGTTCTTACGCGGAAGACTACACGAAACACCACAGGAGACCATCCAAGTTCTTGATGTTGTTCTTAGAGCTGCACCATCACAGAAATACTGTGTGGTTGGTAGGTCATTTTTTAGCCCTGATCTGGGTCCTCGAGGTGACCTTGAATTTGGCTTGGAATACTGGAGAGGCTTTTACCAAAGCCTAAGGCCAACTCAGTTTGGTCTATCCCTAAATATTGATATATCAGCAAGGGCTTTTTATGATCCGATTCTGGTGACAGAGTTTATTAAGAAACATTTTGGGTACAGAGATTTATCCAGGGGTCTGTCGGATCGTGATTGTCTGAAGCTGAAGAAAAATCTGAAAGGAGTCAAGGTAACTCTGAGCTATGGAGGCAGCAGAAGTTATCGAATTACTGGAGTATCTGTGCAGCCACTAAGCCAGTTAACGTTTACTCTTGAGGATAATCAAACCAGAGTATCAGTGGTTCAGTATTATCGTGAGAAGTACAACATTAGCCTAAAACAGGTGGCTTTGCCTGCCCTTCAAGCTGGCAATGATTCAAGGCCTGCTTATTTGCCCATGGAGCTCGCTTCAATTGTTCCTGGGCAGAGATACACGAAGAGGCTCAATGAAAAGCAAGTGACAAACGTGCTGAGAGCAACTTGCCAACGGCCCCGGGAGAGGGAATCCAATATACTGAAGATGGTGATGCACAATAATTACAAGGGAGAGGATCTTGTAAACAAAGAATTTGGAATACAAGTGAAAGAACAAATGGCACTGGTGGATGCTAGAGTATTACCACCTCCAATGCTTAAGTATCATGAGAGTGGTCGTGAAACGAAGGATACTCCCAGGATGGGTGCATGGAACATGATCAATAAGAAAATGGTTAATGGTGCCAAAGTGGATTTTTGGGCTTTTGTCAATTTCTCTCGATCACGTGAAGATTTTAACTATCGCTTTTGTGAGGAACTCGTCTATATGTGCAACAGTAAAGGGGTGGAGTTCCATTCCGAGCCTTTACTTCCTGTACAGTCAGCTCATCCTAATCAAATTGAGAGGGTTCTGAGAGATATTCACAGGCAGTCAGTCCAGAGACTTAAGGATATGGGACAAGCCAGCAAACATCTTCAGTTGTTGATTATCATCTTGCCTGATGTCAAAGGATCTTATGGTCCCATTAAAAGAATTTGTGAAACAGAGCTTGGAATTGTCTCCCAGTGCTGTAATCCTAAGCAAGCACAAAAGCTTAGTAAGCAATACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGGTTGGTGGTAGAAACACAGTCTTAAATGATGCCATTCAGAGGAGGATTCCTCTTGTCAGTGATCGTCCTACAATCATCCTTGGAGCTGATGTCACTCATCCACAAGCTGGGGAAGACAGTAGCCCATCTATAGCAGCAGTGGTTGCCTCAATGGACTGGCCAGAGGTCTCCAAGTATAGAGGAATCCTGTCAGCACAGACTCATCGTCAGGAAATCATCCAAGATCTTTACAAATTAACTCACGATGAGAAAAGAGGGCCGGTTCATTCAGGAATGATCAGAGAACTTTTCATTGCATTCCGAAGATCGACTGGCCAAAAGCCGCACAGAATCATCTTCTATAGGGATGGGGTGAGTGAAGGGCAATTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACGGAAGGCCTGTGCTTCTTTGGAGGAGGGGTATATGCCACCAGTTACTTTTGTTGTGGTGCAGAAAAGGCATCACACACGCCTTTTCCCTGTTGACAACCAGACAGATAGGAGTGGGAATATTCAACCAGGTACTGTGGTGGATACCAAGATCTGCCATCCAACAGAATTTGATTTCTATCTTAATAGCCATGCAGGAATTCAGGGAACAAGTCGCCCAGCTCATTATCATGTCCTGTTTGATGAGAACAACTTCACTGCTGATGGCTTGCAGATTCTCACCAACAATCTGTGCTACACATATGCAAGGTGCACCCGCTCAGTTTCCATAGTGCCTCCTGCCTATTATGCACATCTAGCTGCGTTTAGAGCACGCTATTACATTGAGGGCGATTCATCTGATGGTGCTTCTTCAGTTGGCAGAGGCAGCGGTGGCAGTGCTGCACAAACCCAGGTCCTTCCAAATGTGCACGAGTATGTCAAGGATGTGATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoN PAZ Piwi

Protein Analysis

800

Amino Acids

90.86

Weight (kDa)

9.35

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 3 - 93 3.3e-20 N-terminal domain of argonaute
ArgoL1 PF08699 104 - 152 2.7e-18 Argonaute linker 1 domain
PAZ PF02170 180 - 283 9.7e-27 PAZ domain
ArgoL2 PF16488 293 - 340 1.4e-13 Argonaute linker 2 domain
ArgoMid PF16487 350 - 428 2.4e-09 Mid domain of argonaute
Piwi PF02171 447 - 761 1.5e-107 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AbsI CCTCGAGG 1 cut(s) 345
AccB1I GGYRCC 1 cut(s) 1097
AccB7I CCANNNNNTGG 1 cut(s) 1604
AccBSI CCGCTC 1 cut(s) 2217
AccII CGCG 1 cut(s) 225
AciI CCGC 4 cut(s) 225, 1820, 2215, 2328
AclI AACGTT 1 cut(s) 644
AclWI GGATC 5 cut(s) 142, 452, 534, 939, 1375
AcoI YGGCCR 2 cut(s) 1655, 1810
AcsI RAATTY 5 cut(s) 359, 947, 1389, 2066, 2097
AcuI CTGAAG 5 cut(s) 560, 566, 917, 1316, 2295
AcvI CACGTG 1 cut(s) 1142
AfaI GTAC 5 cut(s) 5, 500, 704, 1233, 2032
AfiI CCNNNNNNNGG 8 cut(s) 337, 338, 721, 879, 880, 1058, 1536, 1604
AflII CTTAAG 3 cut(s) 1013, 1301, 1481
AgsI TTSAA 8 cut(s) 90, 166, 359, 743, 759, 792, 1469, 2024
AhdI GACNNNNNGTC 1 cut(s) 1701
AjnI CCWGG 5 cut(s) 512, 799, 1052, 2026, 2349
AjuI GAANNNNNNNTTGG 2 cut(s) 1389, 1421
Alw21I GWGCWC 4 cut(s) 786, 2213, 2272, 2370
Alw26I GTCTC 7 cut(s) 18, 153, 245, 1291, 1420, 1482, 1669
Alw44I GTGCAC 2 cut(s) 2209, 2366
AlwI GGATC 5 cut(s) 142, 452, 534, 939, 1375
AlwNI CAGNNNCTG 1 cut(s) 2033
Ama87I CYCGRG 2 cut(s) 345, 873
AoxI GGCC 8 cut(s) 71, 401, 761, 869, 1655, 1754, 1810, 1905
ApaLI GTGCAC 2 cut(s) 2209, 2366
ApeKI GCWGC 8 cut(s) 211, 284, 591, 625, 1631, 2258, 2325, 2339
ApoI RAATTY 5 cut(s) 359, 947, 1389, 2066, 2097
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AseI ATTAAT 1 cut(s) 1488
Asp700I GAANNNNTTC 1 cut(s) 2150
AspS9I GGNCC 6 cut(s) 71, 341, 870, 1376, 1754, 2353
AsuC2I CCSGG 3 cut(s) 70, 874, 875
AsuHPI GGTGA 5 cut(s) 362, 481, 916, 1861, 2170
AvaI CYCGRG 2 cut(s) 345, 873
AvaII GGWCC 3 cut(s) 341, 1376, 2353
AxyI CCTNAGG 1 cut(s) 398
BaeGI GKGCMC 2 cut(s) 2213, 2370
BaeI ACNNNNGTAYC 6 cut(s) 600, 633, 657, 690, 2022, 2055
BalI TGGCCA 2 cut(s) 1657, 1812
BanI GGYRCC 1 cut(s) 1097
BanII GRGCYC 1 cut(s) 786
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
BauI CACGAG 1 cut(s) 2369
BbrPI CACGTG 1 cut(s) 1142
BbsI GAAGAC 2 cut(s) 235, 1617
Bbv12I GWGCWC 4 cut(s) 786, 2213, 2272, 2370
BbvI GCAGC 8 cut(s) 223, 271, 603, 637, 1643, 2245, 2326, 2337
BceAI ACGGC 1 cut(s) 884
BciT130I CCWGG 5 cut(s) 514, 801, 1054, 2028, 2351
BciVI GTATCC 2 cut(s) 1039, 2035
BclI TGATCA 2 cut(s) 1074, 1773
BcnI CCSGG 3 cut(s) 70, 874, 875
BcoDI GTCTC 7 cut(s) 18, 153, 245, 1291, 1420, 1482, 1669
BfaI CTAG 2 cut(s) 990, 2255
BfmI CTRYAG 3 cut(s) 209, 1626, 1837
BfrI CTTAAG 3 cut(s) 1013, 1301, 1481
BfuI GTATCC 2 cut(s) 1039, 2035
BglII AGATCT 2 cut(s) 1720, 2049
BisI GCNGC 9 cut(s) 212, 285, 592, 626, 1632, 1820, 2259, 2326, 2340
BlsI GCNGC 9 cut(s) 213, 286, 593, 627, 1633, 1821, 2260, 2327, 2341
Bme1390I CCNGG 8 cut(s) 70, 514, 801, 874, 875, 1054, 2028, 2351
Bme18I GGWCC 3 cut(s) 341, 1376, 2353
BmeRI GACNNNNNGTC 1 cut(s) 1701
BmeT110I CYCGRG 2 cut(s) 345, 873
BmgT120I GGNCC 6 cut(s) 71, 341, 870, 1376, 1754, 2353
BmiI GGNNCC 4 cut(s) 342, 872, 1099, 1378
BmrFI CCNGG 8 cut(s) 70, 514, 801, 874, 875, 1054, 2028, 2351
BmrI ACTGGG 1 cut(s) 1414
BmsI GCATC 6 cut(s) 149, 897, 976, 1516, 1882, 1978
BmuI ACTGGG 1 cut(s) 1414
BpiI GAAGAC 2 cut(s) 235, 1617
BplI GAGNNNNNCTC 2 cut(s) 1531, 1563
BpmI CTGGAG 2 cut(s) 397, 633
Bpu10I CCTNAGC 1 cut(s) 1434
BpuEI CTTGAG 1 cut(s) 674
BpuMI CCSGG 3 cut(s) 70, 874, 875
BsaAI YACGTR 1 cut(s) 1142
BsaBI GATNNNNATC 3 cut(s) 139, 1346, 2076
BsaI GGTCTC 2 cut(s) 245, 1669
BsaJI CCNNGG 8 cut(s) 513, 777, 800, 872, 873, 1052, 1576, 2349
BsaXI ACNNNNNCTCC 8 cut(s) 555, 585, 606, 636, 1572, 1602, 1913, 1943
Bsc4I CCNNNNNNNGG 8 cut(s) 337, 338, 721, 879, 880, 1058, 1536, 1604
Bse118I RCCGGY 1 cut(s) 1756
Bse1I ACTGG 8 cut(s) 380, 616, 637, 984, 1420, 1658, 1813, 1940
Bse21I CCTNAGG 1 cut(s) 398
Bse3DI GCAATG 3 cut(s) 74, 757, 1788
Bse8I GATNNNNATC 3 cut(s) 139, 1346, 2076
BseBI CCWGG 5 cut(s) 514, 801, 1054, 2028, 2351
BseDI CCNNGG 8 cut(s) 513, 777, 800, 872, 873, 1052, 1576, 2349
BseJI GATNNNNATC 3 cut(s) 139, 1346, 2076
BseLI CCNNNNNNNGG 8 cut(s) 337, 338, 721, 879, 880, 1058, 1536, 1604
BseMI GCAATG 3 cut(s) 74, 757, 1788
BseMII CTCAG 6 cut(s) 105, 422, 569, 842, 1259, 2232
BseNI ACTGG 8 cut(s) 380, 616, 637, 984, 1420, 1658, 1813, 1940
BseRI GAGGAG 2 cut(s) 1552, 1937
BseSI GKGCMC 2 cut(s) 2213, 2370
BseXI GCAGC 8 cut(s) 223, 271, 603, 637, 1643, 2245, 2326, 2337
BseYI CCCAGC 2 cut(s) 1604, 2116
BsgI GTGCAG 4 cut(s) 270, 644, 1979, 2325
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 8 cut(s) 73, 403, 763, 871, 1657, 1756, 1812, 1907
BshNI GGYRCC 1 cut(s) 1097
BsiHKAI GWGCWC 4 cut(s) 786, 2213, 2272, 2370
BsiHKCI CYCGRG 2 cut(s) 345, 873
BsiSI CCGG 3 cut(s) 69, 874, 1757
BslFI GGGAC 2 cut(s) 1326, 1362
BslI CCNNNNNNNGG 8 cut(s) 337, 338, 721, 879, 880, 1058, 1536, 1604
BsmAI GTCTC 7 cut(s) 18, 153, 245, 1291, 1420, 1482, 1669
BsmFI GGGAC 2 cut(s) 1326, 1362
BsmI GAATGC 2 cut(s) 36, 1793
BsnI GGCC 8 cut(s) 73, 403, 763, 871, 1657, 1756, 1812, 1907
Bso31I GGTCTC 2 cut(s) 245, 1669
BsoBI CYCGRG 2 cut(s) 345, 873
Bsp1286I GDGCHC 4 cut(s) 786, 2213, 2272, 2370
Bsp1407I TGTACA 1 cut(s) 1231
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 4 cut(s) 225, 1820, 2215, 2328
BspANI GGCC 8 cut(s) 73, 403, 763, 871, 1657, 1756, 1812, 1907
BspCNI CTCAG 6 cut(s) 106, 421, 570, 843, 1260, 2231
BspFNI CGCG 1 cut(s) 225
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 4 cut(s) 342, 872, 1099, 1378
BspMAI CTGCAG 1 cut(s) 213
BspPI GGATC 5 cut(s) 142, 452, 534, 939, 1375
BspT107I GGYRCC 1 cut(s) 1097
BspTI CTTAAG 3 cut(s) 1013, 1301, 1481
BspTNI GGTCTC 2 cut(s) 245, 1669
BsrBI CCGCTC 1 cut(s) 2217
BsrDI GCAATG 3 cut(s) 74, 757, 1788
BsrFI RCCGGY 1 cut(s) 1756
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 8 cut(s) 380, 616, 637, 984, 1420, 1658, 1813, 1940
BssAI RCCGGY 1 cut(s) 1756
BssECI CCNNGG 8 cut(s) 513, 777, 800, 872, 873, 1052, 1576, 2349
BssSI CACGAG 1 cut(s) 2369
BssT1I CCWWGG 2 cut(s) 777, 1576
Bst2BI CACGAG 1 cut(s) 2369
Bst2UI CCWGG 5 cut(s) 514, 801, 1054, 2028, 2351
Bst4CI ACNGT 6 cut(s) 305, 1193, 1236, 1516, 1616, 2035
Bst6I CTCTTC 3 cut(s) 47, 91, 812
BstAFI CTTAAG 3 cut(s) 1013, 1301, 1481
BstAPI GCANNNNNTGC 1 cut(s) 1640
BstAUI TGTACA 1 cut(s) 1231
BstBAI YACGTR 1 cut(s) 1142
BstC8I GCNNGC 9 cut(s) 36, 735, 748, 765, 786, 1321, 1440, 2168, 2272
BstDSI CCRYGG 1 cut(s) 777
BstEII GGTNACC 1 cut(s) 350
BstENI CCTNNNNNAGG 1 cut(s) 719
BstFNI CGCG 1 cut(s) 225
BstMAI GTCTC 7 cut(s) 18, 153, 245, 1291, 1420, 1482, 1669
BstNI CCWGG 5 cut(s) 514, 801, 1054, 2028, 2351
BstPI GGTNACC 1 cut(s) 350
BstSCI CCNGG 8 cut(s) 68, 512, 799, 872, 873, 1052, 2026, 2349
BstSFI CTRYAG 3 cut(s) 209, 1626, 1837
BstSLI GKGCMC 2 cut(s) 2213, 2370
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 8 cut(s) 223, 271, 603, 637, 1643, 2245, 2326, 2337
BstV2I GAAGAC 2 cut(s) 235, 1617
BstX2I RGATCY 5 cut(s) 134, 931, 1367, 1720, 2049
BstYI RGATCY 5 cut(s) 134, 931, 1367, 1720, 2049
Bsu36I CCTNAGG 1 cut(s) 398
BsuI GTATCC 2 cut(s) 1039, 2035
BsuRI GGCC 8 cut(s) 73, 403, 763, 871, 1657, 1756, 1812, 1907
BtgI CCRYGG 1 cut(s) 777
BtsI GCAGTG 4 cut(s) 63, 1641, 2154, 2341
BtsIMutI CAGTG 8 cut(s) 63, 684, 977, 1427, 1561, 1641, 2154, 2341
Cac8I GCNNGC 9 cut(s) 36, 735, 748, 765, 786, 1321, 1440, 2168, 2272
CaiI CAGNNNCTG 1 cut(s) 2033
CciI TCATGA 1 cut(s) 1021
Cfr10I RCCGGY 1 cut(s) 1756
Cfr13I GGNCC 6 cut(s) 71, 341, 870, 1376, 1754, 2353
Cfr9I CCCGGG 1 cut(s) 873
CsiI ACCWGGT 1 cut(s) 2026
Csp6I GTAC 5 cut(s) 4, 499, 703, 1232, 2031
CspCI CAANNNNNGTGG 2 cut(s) 1483, 1518
CviAII CATG 7 cut(s) 49, 778, 1022, 1065, 1072, 2090, 2129
CviQI GTAC 5 cut(s) 4, 499, 703, 1232, 2031
DrdI GACNNNNNNGTC 1 cut(s) 167
DriI GACNNNNNGTC 1 cut(s) 1701
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 1655, 1810
Eam1104I CTCTTC 3 cut(s) 47, 91, 812
Eam1105I GACNNNNNGTC 1 cut(s) 1701
EarI CTCTTC 3 cut(s) 47, 91, 812
Ecl136II GAGCTC 1 cut(s) 784
Eco130I CCWWGG 2 cut(s) 777, 1576
Eco147I AGGCCT 2 cut(s) 763, 1907
Eco24I GRGCYC 1 cut(s) 786
Eco31I GGTCTC 2 cut(s) 245, 1669
Eco47I GGWCC 3 cut(s) 341, 1376, 2353
Eco53kI GAGCTC 1 cut(s) 784
Eco57I CTGAAG 5 cut(s) 560, 566, 917, 1316, 2295
Eco72I CACGTG 1 cut(s) 1142
Eco81I CCTNAGG 1 cut(s) 398
Eco88I CYCGRG 2 cut(s) 345, 873
Eco91I GGTNACC 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 784
EcoNI CCTNNNNNAGG 1 cut(s) 719
EcoO109I RGGNCCY 2 cut(s) 341, 2353
EcoO65I GGTNACC 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 2097
EcoRII CCWGG 5 cut(s) 512, 799, 1052, 2026, 2349
EcoT14I CCWWGG 2 cut(s) 777, 1576
EcoT38I GRGCYC 1 cut(s) 786
ErhI CCWWGG 2 cut(s) 777, 1576
FaeI CATG 7 cut(s) 52, 781, 1025, 1068, 1075, 2093, 2132
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 2 cut(s) 1326, 1362
FatI CATG 7 cut(s) 48, 777, 1021, 1064, 1071, 2089, 2128
FauI CCCGC 1 cut(s) 2222
FauNDI CATATG 2 cut(s) 40, 2200
FbaI TGATCA 2 cut(s) 1074, 1773
Fnu4HI GCNGC 9 cut(s) 212, 285, 592, 626, 1632, 1820, 2259, 2326, 2340
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 9 cut(s) 212, 285, 592, 626, 1632, 1820, 2259, 2326, 2340
FspBI CTAG 2 cut(s) 990, 2255
GluI GCNGC 9 cut(s) 212, 285, 592, 626, 1632, 1820, 2259, 2326, 2340
GsaI CCCAGC 2 cut(s) 1608, 2120
GsuI CTGGAG 2 cut(s) 397, 633
HaeIII GGCC 8 cut(s) 73, 403, 763, 871, 1657, 1756, 1812, 1907
HapII CCGG 3 cut(s) 69, 874, 1757
Hin1II CATG 7 cut(s) 52, 781, 1025, 1068, 1075, 2093, 2132
HincII GTYRAC 2 cut(s) 642, 1993
HindII GTYRAC 2 cut(s) 642, 1993
HindIII AAGCTT 1 cut(s) 1448
HpaI GTTAAC 1 cut(s) 642
HpaII CCGG 3 cut(s) 69, 874, 1757
HphI GGTGA 5 cut(s) 362, 481, 916, 1861, 2170
Hpy166II GTNNAC 6 cut(s) 642, 648, 940, 1993, 2211, 2368
Hpy8I GTNNAC 6 cut(s) 642, 648, 940, 1993, 2211, 2368
HpyAV CCTTC 8 cut(s) 91, 116, 141, 749, 1036, 1850, 1897, 2366
HpyCH4III ACNGT 6 cut(s) 305, 1193, 1236, 1516, 1616, 2035
HpyCH4IV ACGT 3 cut(s) 644, 846, 1141
HpySE526I ACGT 3 cut(s) 644, 846, 1141
Hsp92II CATG 7 cut(s) 52, 781, 1025, 1068, 1075, 2093, 2132
Ksp22I TGATCA 2 cut(s) 1074, 1773
KspAI GTTAAC 1 cut(s) 642
LmnI GCTCC 2 cut(s) 781, 1580
Lsp1109I GCAGC 8 cut(s) 223, 271, 603, 637, 1643, 2245, 2326, 2337
LweI GCATC 6 cut(s) 149, 897, 976, 1516, 1882, 1978
MabI ACCWGGT 1 cut(s) 2026
MaeI CTAG 2 cut(s) 990, 2255
MaeII ACGT 3 cut(s) 644, 846, 1141
MaeIII GTNAC 6 cut(s) 350, 469, 571, 838, 1588, 1942
MbiI CCGCTC 1 cut(s) 2217
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 5 cut(s) 134, 931, 1367, 1720, 2049
MhlI GDGCHC 4 cut(s) 786, 2213, 2272, 2370
MlsI TGGCCA 2 cut(s) 1657, 1812
MluNI TGGCCA 2 cut(s) 1657, 1812
MlyI GAGTC 3 cut(s) 20, 573, 1690
MmeI TCCRAC 2 cut(s) 504, 2084
Mox20I TGGCCA 2 cut(s) 1657, 1812
MroXI GAANNNNTTC 1 cut(s) 2150
MscI TGGCCA 2 cut(s) 1657, 1812
MslI CAYNNNNRTG 3 cut(s) 1026, 2127, 2373
Msp20I TGGCCA 2 cut(s) 1657, 1812
MspA1I CMGCKG 1 cut(s) 2328
MspCI CTTAAG 3 cut(s) 1013, 1301, 1481
MspI CCGG 3 cut(s) 69, 874, 1757
MspR9I CCNGG 8 cut(s) 70, 514, 801, 874, 875, 1054, 2028, 2351
MunI CAATTG 1 cut(s) 792
Mva1269I GAATGC 2 cut(s) 36, 1793
MvaI CCWGG 5 cut(s) 514, 801, 1054, 2028, 2351
MvnI CGCG 1 cut(s) 225
NciI CCSGG 3 cut(s) 70, 874, 875
NcoI CCATGG 1 cut(s) 777
NdeI CATATG 2 cut(s) 40, 2200
NlaIII CATG 7 cut(s) 52, 781, 1025, 1068, 1075, 2093, 2132
NlaIV GGNNCC 4 cut(s) 342, 872, 1099, 1378
NmuCI GTSAC 4 cut(s) 350, 469, 838, 1588
PaeR7I CTCGAG 1 cut(s) 345
PagI TCATGA 1 cut(s) 1021
PceI AGGCCT 2 cut(s) 763, 1907
PctI GAATGC 2 cut(s) 36, 1793
PdmI GAANNNNTTC 1 cut(s) 2150
PfeI GAWTC 8 cut(s) 463, 755, 884, 1543, 1680, 1827, 2173, 2291
PflMI CCANNNNNTGG 1 cut(s) 1604
PkrI GCNGC 9 cut(s) 213, 286, 593, 627, 1633, 1821, 2260, 2327, 2341
PleI GAGTC 3 cut(s) 19, 572, 1690
PmaCI CACGTG 1 cut(s) 1142
PmlI CACGTG 1 cut(s) 1142
PpsI GAGTC 3 cut(s) 19, 572, 1690
Ppu21I YACGTR 1 cut(s) 1142
PpuMI RGGWCCY 2 cut(s) 341, 2353
PshBI ATTAAT 1 cut(s) 1488
Psp124BI GAGCTC 1 cut(s) 786
Psp1406I AACGTT 1 cut(s) 644
Psp5II RGGWCCY 2 cut(s) 341, 2353
Psp6I CCWGG 5 cut(s) 512, 799, 1052, 2026, 2349
PspCI CACGTG 1 cut(s) 1142
PspEI GGTNACC 1 cut(s) 350
PspFI CCCAGC 2 cut(s) 1604, 2116
PspGI CCWGG 5 cut(s) 512, 799, 1052, 2026, 2349
PspN4I GGNNCC 4 cut(s) 342, 872, 1099, 1378
PspPI GGNCC 6 cut(s) 71, 341, 870, 1376, 1754, 2353
PspPPI RGGWCCY 2 cut(s) 341, 2353
PspXI VCTCGAGB 1 cut(s) 345
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PstI CTGCAG 1 cut(s) 213
PstNI CAGNNNCTG 1 cut(s) 2033
PsuI RGATCY 5 cut(s) 134, 931, 1367, 1720, 2049
RsaI GTAC 5 cut(s) 5, 500, 704, 1233, 2032
RsaNI GTAC 5 cut(s) 4, 499, 703, 1232, 2031
RseI CAYNNNNRTG 3 cut(s) 1026, 2127, 2373
SacI GAGCTC 1 cut(s) 786
SatI GCNGC 9 cut(s) 212, 285, 592, 626, 1632, 1820, 2259, 2326, 2340
Sau96I GGNCC 6 cut(s) 71, 341, 870, 1376, 1754, 2353
SchI GAGTC 3 cut(s) 20, 573, 1690
ScrFI CCNGG 8 cut(s) 70, 514, 801, 874, 875, 1054, 2028, 2351
SduI GDGCHC 4 cut(s) 786, 2213, 2272, 2370
SexAI ACCWGGT 1 cut(s) 2026
SfaNI GCATC 6 cut(s) 149, 897, 976, 1516, 1882, 1978
SfcI CTRYAG 3 cut(s) 209, 1626, 1837
Sfr274I CTCGAG 1 cut(s) 345
SinI GGWCC 3 cut(s) 341, 1376, 2353
SlaI CTCGAG 1 cut(s) 345
SmaI CCCGGG 1 cut(s) 875
SmiMI CAYNNNNRTG 3 cut(s) 1026, 2127, 2373
SmlI CTYRAG 5 cut(s) 345, 653, 1013, 1301, 1481
SmoI CTYRAG 5 cut(s) 345, 653, 1013, 1301, 1481
SseBI AGGCCT 2 cut(s) 763, 1907
SsiI CCGC 4 cut(s) 225, 1820, 2215, 2328
SspI AATATT 2 cut(s) 430, 2020
SspMI CTAG 2 cut(s) 990, 2255
SstI GAGCTC 1 cut(s) 786
StuI AGGCCT 2 cut(s) 763, 1907
StyD4I CCNGG 8 cut(s) 68, 512, 799, 872, 873, 1052, 2026, 2349
StyI CCWWGG 2 cut(s) 777, 1576
TaaI ACNGT 6 cut(s) 305, 1193, 1236, 1516, 1616, 2035
TaiI ACGT 3 cut(s) 647, 849, 1144
TaqI TCGA 5 cut(s) 127, 346, 604, 1135, 1805
TatI WGTACW 3 cut(s) 3, 702, 1231
TauI GCSGC 1 cut(s) 1822
TfiI GAWTC 8 cut(s) 463, 755, 884, 1543, 1680, 1827, 2173, 2291
TscAI CASTG 8 cut(s) 70, 684, 984, 1427, 1561, 1641, 2161, 2341
TseFI GTSAC 4 cut(s) 350, 469, 838, 1588
TseI GCWGC 8 cut(s) 211, 284, 591, 625, 1631, 2258, 2325, 2339
Tsp45I GTSAC 4 cut(s) 350, 469, 838, 1588
TspDTI ATGAA 5 cut(s) 65, 843, 1751, 1777, 2283
TspGWI ACGGA 1 cut(s) 1915
TspMI CCCGGG 1 cut(s) 873
TspRI CASTG 8 cut(s) 70, 684, 984, 1427, 1561, 1641, 2161, 2341
Van91I CCANNNNNTGG 1 cut(s) 1604
Vha464I CTTAAG 3 cut(s) 1013, 1301, 1481
VneI GTGCAC 2 cut(s) 2209, 2366
VpaK11BI GGWCC 3 cut(s) 341, 1376, 2353
VspI ATTAAT 1 cut(s) 1488
XagI CCTNNNNNAGG 1 cut(s) 719
XapI RAATTY 5 cut(s) 359, 947, 1389, 2066, 2097
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XhoI CTCGAG 1 cut(s) 345
XmaI CCCGGG 1 cut(s) 873
XmnI GAANNNNTTC 1 cut(s) 2150
XspI CTAG 2 cut(s) 990, 2255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.