FvH4_4g09990

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000521
Physical Location & Seq
Forward (+)
12319063 .. 12322675
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g09990.t1

Sequence Viewer

Length: 1491 bp
ATGGCTGACGAAGAACATTCAGCTGGTCTTGAATCACCCTTGATTCTAGTTCAGCCCCATGAACATACATTACAATCAACAAAGGGACGATTCACGAAAGGCGAATATTTCGGGGAACTGAAGAAGCAGTTATTGTTGGCAGGGCCGATGGTATCATCAAATTTCTTGTTGTTTGGTATGCAGGTTATTTCAGTCATGTATGTTGGTCATCTTGGGGAGCTATCACTTGCAGGTGCTTCTATGGCCACTTCTTTTGCATCTGTGACTGGTTTGAGCGTGATAATAGGAATGGGTAGCGCATTAGACACATTTTGCGGACAGTCATATGGAGCAAAACAATATCATATGCTTGGTATACACTTGCAGAGGGCAATGCTTGTGCTTCTGCTGGCCAGCATTCCTCTTGCTACTATATGGTTCAATGCAGGCCATATTCTTAAATTCTTGGGTCAAGATCAAGAAATTGCTGCATCTGCTGGAAACTATGCTCGTTTCTTGATACCTTGCATTTTCGCTTACGCAGTCCAACAATGTCATTCTAGATTCTTGCAAACTCAAAACAATGTGGTTCCTATGATAGTTAGCACAGGCACAGCAACTCTAGTACACTTGCTTTTGTGTTGGCTTCTCGTATATAAGACATGCCTTGGATATAGAGGAGCTGCGGTCGCAATCTCCATCTCGTATTGGCTCAATGCATTGTTCTTGGTTGTATATATCAGAGTTTCTTCCTGTTGTAAACACACATGGACTGGTTTTTCGAAGGAGGCATTCCGTGGAATTCCCACCTTCATAAAATTATCTATTCCTTCAGCTTTAATGATCAGCCTAGAAATATGGTCATTCGAATTGATGGTCCTCTTGTCTGGTTTTCTTCCAAATCCACAGCTTGAAACCTCAGTTCTATCAATCAGCCTTAACACATGCTCCATGGTATACATGATTCCTCTTGCATTCGCCGGTGCAGCCAGCACAAGAGTTTCAAATCAATTGGGTGCTGGGGAACCACGACTAGCTCGCCTAGCAGTAGGTGTTGCACTATGCATTGTTGTTACTGAAGGCTTTGTGACTGGTGCAATAATGATACTAGGCCGAAAAGTTTGGGGCTACTGCTATAGCAGTGAAAAGGAAGTTATCAGTTATGTTGGGCAAATGCTTATTTTGGTTGCAGTATCCCACTTTTTTGATGGGCTTCAATCTGTGCTTTCAGGTATTATAAGAGGAAGTGGACAGCAAAATATTGGAGCATATGTTAACCTGGGAGCTTATTATGTTATGGGAATTCCTACTGCAGTAGTATTAGCATTTGTATTCCACATTGGAGGAAAGGGTCTCTGGATGGGAATCATTGTTGCGCTATTTGTGCAAGCACTGTCTCTTGCGATCATAATTTTATTTACAGACTGGGAGAAAGAAGTGAAGAAAGCTTCTGATAGAGTACACAACAGCGACAACAGACCAACCGCGGCTGATACATCGTCGCTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

497

Amino Acids

53.7

Weight (kDa)

8.45

Isoelectric Point (pI)

25.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 49 - 209 3.6e-39 MatE
MatE PF01554 270 - 432 4.7e-25 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1217
AarI CACCTGC 1 cut(s) 221
Acc36I ACCTGC 2 cut(s) 172, 221
AccI GTMKAC 2 cut(s) 355, 936
AccII CGCG 1 cut(s) 1466
AciI CCGC 4 cut(s) 315, 665, 1464, 1466
AcoI YGGCCR 2 cut(s) 243, 390
AcsI RAATTY 4 cut(s) 160, 440, 780, 1281
AcuI CTGAAG 3 cut(s) 140, 795, 1077
AfaI GTAC 2 cut(s) 606, 1440
AgsI TTSAA 5 cut(s) 32, 421, 893, 984, 1196
AjnI CCWGG 1 cut(s) 1257
AluBI AGCT 9 cut(s) 23, 220, 662, 815, 889, 1016, 1265, 1427, 1488
AluI AGCT 9 cut(s) 23, 220, 662, 815, 889, 1016, 1265, 1427, 1488
Alw26I GTCTC 2 cut(s) 1337, 1380
AoxI GGCC 5 cut(s) 143, 243, 390, 427, 1090
ApeKI GCWGC 3 cut(s) 467, 662, 965
ApoI RAATTY 4 cut(s) 160, 440, 780, 1281
ArsI GACNNNNNNTTYG 4 cut(s) 742, 774, 840, 872
AspLEI GCGC 2 cut(s) 299, 1357
AspS9I GGNCC 2 cut(s) 143, 856
AsuHPI GGTGA 1 cut(s) 27
AsuII TTCGAA 2 cut(s) 761, 846
AvaII GGWCC 1 cut(s) 856
BalI TGGCCA 2 cut(s) 245, 392
BbvI GCAGC 3 cut(s) 454, 649, 977
BccI CCATC 5 cut(s) 142, 686, 847, 1181, 1333
BciT130I CCWGG 1 cut(s) 1259
BciVI GTATCC 1 cut(s) 1183
BclI TGATCA 1 cut(s) 822
BcoDI GTCTC 2 cut(s) 1337, 1380
BfaI CTAG 7 cut(s) 47, 540, 602, 830, 1013, 1022, 1088
BfmI CTRYAG 2 cut(s) 1114, 1290
BfuAI ACCTGC 2 cut(s) 172, 221
BfuI GTATCC 1 cut(s) 1183
BisI GCNGC 4 cut(s) 468, 663, 966, 1467
BlpI GCTNAGC 1 cut(s) 1484
BlsI GCNGC 4 cut(s) 469, 664, 967, 1468
Bme1390I CCNGG 1 cut(s) 1259
Bme18I GGWCC 1 cut(s) 856
BmgT120I GGNCC 2 cut(s) 143, 856
BmiI GGNNCC 2 cut(s) 570, 1005
BmrFI CCNGG 1 cut(s) 1259
BmrI ACTGGG 1 cut(s) 1414
BmsI GCATC 2 cut(s) 266, 479
BmuI ACTGGG 1 cut(s) 1414
Bpu1102I GCTNAGC 1 cut(s) 1484
Bpu14I TTCGAA 2 cut(s) 761, 846
BsaBI GATNNNNATC 1 cut(s) 1343
BsaI GGTCTC 1 cut(s) 1337
BsaJI CCNNGG 5 cut(s) 646, 775, 930, 1258, 1464
BsaXI ACNNNNNCTCC 6 cut(s) 651, 681, 911, 941, 1236, 1266
Bse118I RCCGGY 1 cut(s) 959
Bse1I ACTGG 4 cut(s) 271, 757, 1075, 1409
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 1343
BseBI CCWGG 1 cut(s) 1259
BseDI CCNNGG 5 cut(s) 646, 775, 930, 1258, 1464
BseGI GGATG 1 cut(s) 1344
BseJI GATNNNNATC 1 cut(s) 1343
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 912, 1475
BseNI ACTGG 4 cut(s) 271, 757, 1075, 1409
BseRI GAGGAG 1 cut(s) 672
BseXI GCAGC 3 cut(s) 454, 649, 977
BseYI CCCAGC 1 cut(s) 998
BsgI GTGCAG 1 cut(s) 984
Bsh1236I CGCG 1 cut(s) 1466
Bsh1285I CGRYCG 1 cut(s) 669
BshFI GGCC 5 cut(s) 145, 245, 392, 429, 1092
BsiEI CGRYCG 1 cut(s) 669
BsiSI CCGG 1 cut(s) 960
BslFI GGGAC 1 cut(s) 99
BsmAI GTCTC 2 cut(s) 1337, 1380
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 3 cut(s) 396, 770, 953
BsnI GGCC 5 cut(s) 145, 245, 392, 429, 1092
Bso31I GGTCTC 1 cut(s) 1337
Bsp119I TTCGAA 2 cut(s) 761, 846
Bsp143I GATC 3 cut(s) 454, 822, 1383
Bsp1720I GCTNAGC 1 cut(s) 1484
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 4 cut(s) 315, 665, 1464, 1466
BspANI GGCC 5 cut(s) 145, 245, 392, 429, 1092
BspCNI CTCAG 2 cut(s) 911, 1476
BspFNI CGCG 1 cut(s) 1466
BspLI GGNNCC 2 cut(s) 570, 1005
BspMAI CTGCAG 1 cut(s) 1294
BspMI ACCTGC 2 cut(s) 172, 221
BspT104I TTCGAA 2 cut(s) 761, 846
BspTNI GGTCTC 1 cut(s) 1337
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 959
BsrI ACTGG 4 cut(s) 271, 757, 1075, 1409
BssAI RCCGGY 1 cut(s) 959
BssECI CCNNGG 5 cut(s) 646, 775, 930, 1258, 1464
BssMI GATC 3 cut(s) 454, 822, 1383
BssNAI GTATAC 2 cut(s) 356, 937
BssT1I CCWWGG 2 cut(s) 646, 930
Bst1107I GTATAC 2 cut(s) 356, 937
Bst2UI CCWGG 1 cut(s) 1259
Bst4CI ACNGT 2 cut(s) 321, 1374
BstBI TTCGAA 2 cut(s) 761, 846
BstC8I GCNNGC 6 cut(s) 390, 394, 427, 970, 1018, 1368
BstDEI CTNAG 2 cut(s) 898, 1484
BstDSI CCRYGG 3 cut(s) 775, 930, 1464
BstF5I GGATG 1 cut(s) 1344
BstFNI CGCG 1 cut(s) 1466
BstHHI GCGC 2 cut(s) 299, 1357
BstKTI GATC 3 cut(s) 457, 825, 1386
BstMAI GTCTC 2 cut(s) 1337, 1380
BstMBI GATC 3 cut(s) 454, 822, 1383
BstMCI CGRYCG 1 cut(s) 669
BstMWI GCNNNNNNNGC 6 cut(s) 242, 473, 668, 965, 1022, 1363
BstNI CCWGG 1 cut(s) 1259
BstNSI RCATGY 2 cut(s) 645, 927
BstSCI CCNGG 1 cut(s) 1257
BstSFI CTRYAG 2 cut(s) 1114, 1290
BstUI CGCG 1 cut(s) 1466
BstV1I GCAGC 3 cut(s) 454, 649, 977
BstZ17I GTATAC 2 cut(s) 356, 937
BsuI GTATCC 1 cut(s) 1183
BsuRI GGCC 5 cut(s) 145, 245, 392, 429, 1092
BtgI CCRYGG 3 cut(s) 775, 930, 1464
BtsCI GGATG 1 cut(s) 1344
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 2 cut(s) 1126, 1370
BveI ACCTGC 2 cut(s) 172, 221
Cac8I GCNNGC 6 cut(s) 390, 394, 427, 970, 1018, 1368
CfoI GCGC 2 cut(s) 299, 1357
Cfr10I RCCGGY 1 cut(s) 959
Cfr13I GGNCC 2 cut(s) 143, 856
Cfr42I CCGCGG 1 cut(s) 1467
Csp6I GTAC 2 cut(s) 605, 1439
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 7 cut(s) 59, 196, 642, 747, 924, 931, 940
CviQI GTAC 2 cut(s) 605, 1439
DdeI CTNAG 2 cut(s) 898, 1484
DpnI GATC 3 cut(s) 456, 824, 1385
DpnII GATC 3 cut(s) 454, 822, 1383
EaeI YGGCCR 2 cut(s) 243, 390
Eco130I CCWWGG 2 cut(s) 646, 930
Eco31I GGTCTC 1 cut(s) 1337
Eco47I GGWCC 1 cut(s) 856
Eco57I CTGAAG 3 cut(s) 140, 795, 1077
EcoRI GAATTC 2 cut(s) 780, 1281
EcoRII CCWGG 1 cut(s) 1257
EcoT14I CCWWGG 2 cut(s) 646, 930
EcoT22I ATGCAT 2 cut(s) 700, 1046
ErhI CCWWGG 2 cut(s) 646, 930
FaeI CATG 7 cut(s) 62, 199, 645, 750, 927, 934, 943
FaqI GGGAC 1 cut(s) 99
FatI CATG 7 cut(s) 58, 195, 641, 746, 923, 930, 939
FauNDI CATATG 3 cut(s) 325, 345, 1249
FbaI TGATCA 1 cut(s) 822
FblI GTMKAC 2 cut(s) 355, 936
Fnu4HI GCNGC 4 cut(s) 468, 663, 966, 1467
FokI GGATG 1 cut(s) 1351
Fsp4HI GCNGC 4 cut(s) 468, 663, 966, 1467
FspBI CTAG 7 cut(s) 47, 540, 602, 830, 1013, 1022, 1088
GlaI GCGC 2 cut(s) 298, 1356
GluI GCNGC 4 cut(s) 468, 663, 966, 1467
GsaI CCCAGC 1 cut(s) 1002
HaeIII GGCC 5 cut(s) 145, 245, 392, 429, 1092
HapII CCGG 1 cut(s) 960
HhaI GCGC 2 cut(s) 299, 1357
Hin1II CATG 7 cut(s) 62, 199, 645, 750, 927, 934, 943
Hin6I GCGC 2 cut(s) 297, 1355
HinP1I GCGC 2 cut(s) 297, 1355
HincII GTYRAC 1 cut(s) 1255
HindII GTYRAC 1 cut(s) 1255
HindIII AAGCTT 1 cut(s) 1425
HinfI GANTC 6 cut(s) 32, 43, 90, 543, 943, 1344
HpaI GTTAAC 1 cut(s) 1255
HpaII CCGG 1 cut(s) 960
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 7 cut(s) 356, 607, 740, 937, 1229, 1255, 1441
Hpy188I TCNGA 2 cut(s) 722, 1432
Hpy188III TCNNGA 7 cut(s) 29, 94, 452, 458, 496, 540, 1336
Hpy8I GTNNAC 7 cut(s) 356, 607, 740, 937, 1229, 1255, 1441
Hpy99I CGWCG 1 cut(s) 1483
HpyAV CCTTC 4 cut(s) 757, 799, 819, 1052
HpyCH4III ACNGT 2 cut(s) 321, 1374
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 473, 668, 965, 1022, 1363
HpyF3I CTNAG 2 cut(s) 898, 1484
Hsp92II CATG 7 cut(s) 62, 199, 645, 750, 927, 934, 943
HspAI GCGC 2 cut(s) 297, 1355
Ksp22I TGATCA 1 cut(s) 822
KspAI GTTAAC 1 cut(s) 1255
KspI CCGCGG 1 cut(s) 1467
Kzo9I GATC 3 cut(s) 454, 822, 1383
LmnI GCTCC 6 cut(s) 217, 329, 659, 932, 1244, 1262
Lsp1109I GCAGC 3 cut(s) 454, 649, 977
LweI GCATC 2 cut(s) 266, 479
MaeI CTAG 7 cut(s) 47, 540, 602, 830, 1013, 1022, 1088
MaeIII GTNAC 3 cut(s) 262, 1051, 1066
MalI GATC 3 cut(s) 456, 824, 1385
MboI GATC 3 cut(s) 454, 822, 1383
MboII GAAGA 5 cut(s) 23, 133, 720, 866, 1432
MfeI CAATTG 1 cut(s) 989
MlsI TGGCCA 2 cut(s) 245, 392
MluCI AATT 9 cut(s) 160, 440, 462, 780, 797, 848, 989, 1281, 1389
MluNI TGGCCA 2 cut(s) 245, 392
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 9 cut(s) 360, 411, 650, 760, 869, 907, 957, 1214, 1316
Mox20I TGGCCA 2 cut(s) 245, 392
Mph1103I ATGCAT 2 cut(s) 700, 1046
MscI TGGCCA 2 cut(s) 245, 392
MseI TTAA 4 cut(s) 438, 818, 918, 1254
Msp20I TGGCCA 2 cut(s) 245, 392
MspA1I CMGCKG 2 cut(s) 23, 1466
MspI CCGG 1 cut(s) 960
MspR9I CCNGG 1 cut(s) 1259
MunI CAATTG 1 cut(s) 989
Mva1269I GAATGC 3 cut(s) 396, 770, 953
MvaI CCWGG 1 cut(s) 1259
MvnI CGCG 1 cut(s) 1466
MwoI GCNNNNNNNGC 6 cut(s) 242, 473, 668, 965, 1022, 1363
NcoI CCATGG 1 cut(s) 930
NdeI CATATG 3 cut(s) 325, 345, 1249
NdeII GATC 3 cut(s) 454, 822, 1383
NlaIII CATG 7 cut(s) 62, 199, 645, 750, 927, 934, 943
NlaIV GGNNCC 2 cut(s) 570, 1005
NmuCI GTSAC 2 cut(s) 262, 1066
NsiI ATGCAT 2 cut(s) 700, 1046
NspI RCATGY 2 cut(s) 645, 927
NspV TTCGAA 2 cut(s) 761, 846
PaqCI CACCTGC 1 cut(s) 221
PctI GAATGC 3 cut(s) 396, 770, 953
PfeI GAWTC 6 cut(s) 32, 43, 90, 543, 943, 1344
PkrI GCNGC 4 cut(s) 469, 664, 967, 1468
PsiI TTATAA 1 cut(s) 1217
Psp6I CCWGG 1 cut(s) 1257
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 1 cut(s) 1257
PspN4I GGNNCC 2 cut(s) 570, 1005
PspPI GGNCC 2 cut(s) 143, 856
PsrI GAACNNNNNNTAC 2 cut(s) 54, 86
PstI CTGCAG 1 cut(s) 1294
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 2 cut(s) 606, 1440
RsaNI GTAC 2 cut(s) 605, 1439
SacII CCGCGG 1 cut(s) 1467
SaqAI TTAA 4 cut(s) 438, 818, 918, 1254
SatI GCNGC 4 cut(s) 468, 663, 966, 1467
Sau3AI GATC 3 cut(s) 454, 822, 1383
Sau96I GGNCC 2 cut(s) 143, 856
ScrFI CCNGG 1 cut(s) 1259
SfaNI GCATC 2 cut(s) 266, 479
SfcI CTRYAG 2 cut(s) 1114, 1290
Sfr303I CCGCGG 1 cut(s) 1467
SfuI TTCGAA 2 cut(s) 761, 846
SgrAI CRCCGGYG 1 cut(s) 959
SgrBI CCGCGG 1 cut(s) 1467
SinI GGWCC 1 cut(s) 856
Sse9I AATT 9 cut(s) 160, 440, 462, 780, 797, 848, 989, 1281, 1389
SsiI CCGC 4 cut(s) 315, 665, 1464, 1466
SspI AATATT 2 cut(s) 107, 1240
SspMI CTAG 7 cut(s) 47, 540, 602, 830, 1013, 1022, 1088
StyD4I CCNGG 1 cut(s) 1257
StyI CCWWGG 2 cut(s) 646, 930
TaaI ACNGT 2 cut(s) 321, 1374
TaqI TCGA 2 cut(s) 761, 846
TasI AATT 9 cut(s) 160, 440, 462, 780, 797, 848, 989, 1281, 1389
TatI WGTACW 2 cut(s) 604, 1438
TauI GCSGC 1 cut(s) 1469
TfiI GAWTC 6 cut(s) 32, 43, 90, 543, 943, 1344
Tru1I TTAA 4 cut(s) 438, 818, 918, 1254
Tru9I TTAA 4 cut(s) 438, 818, 918, 1254
TscAI CASTG 2 cut(s) 1126, 1377
TseFI GTSAC 2 cut(s) 262, 1066
TseI GCWGC 3 cut(s) 467, 662, 965
Tsp45I GTSAC 2 cut(s) 262, 1066
TspDTI ATGAA 2 cut(s) 75, 781
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 2 cut(s) 1126, 1377
VpaK11BI GGWCC 1 cut(s) 856
XapI RAATTY 4 cut(s) 160, 440, 780, 1281
XbaI TCTAGA 1 cut(s) 539
XceI RCATGY 2 cut(s) 645, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 1184
XmiI GTMKAC 2 cut(s) 355, 936
XspI CTAG 7 cut(s) 47, 540, 602, 830, 1013, 1022, 1088
Zsp2I ATGCAT 2 cut(s) 700, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.