Rw7G038620

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000521
Physical Location & Seq
Forward (+)
59085444 .. 59094599
9156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G038620.1

Sequence Viewer

Length: 1428 bp
ATGGATGTAGAAGAAGAAAGATATGATCTTGAATCACTGTTGATTCCTAATTCTCAAGAACATGCTATTGAATCGAGAAAGGGATTCCTGAACAAAGATGAGATTTTTGAGGAAGTGAAGAAGCAGCTGTTGTTAATATGGCCGCTAGTTCATCTTGGAGAGCTATCGCTCGCTAGTGCTTCAATGGCTACTTCATTCGCTTCAGTCACTGGTTTGAGCTTGATACTGGGAATGTGTAGCGCCTTGGACACTTTCTGCGGTCAGTCCTTTGGAGCAAAACAGTATCGGATGCTTGGTATACATATGCAGAGGGCAATGCTTATTCTTTTGCTCGTCAGCGTTCCTCTTGCAATTATATGGGCTAATGCAGGCAATATTCTTCAGTGCTTAGGTCAAGATCCAGAGATATCCTCTGCTGCAGGAGATTATGCTCGTTTGATGATACCTTGCATTTTTGCTTATGCAATCCTACAATGTCATGCTAGATTCTTGCAAACTCAAAGCAATGTGATTCCAATGATTGCTAGCACAGGAACTGCAACACTACTGCACTTGCTTACCTGTTGGGTTATGGTATACAAGACCAGCCTTGGATCTAGAGGTGCTGCTGTGGCAAACGCCATCACCTATTGGATCAATGCATTGTTTTTGTTTGTTTATGTCAGAGTTTCTCCCTCTTGCAAGAACACATGGACTGGATTCTCAAAGGAGGCCTTCCATGGAATTCCCACTTCTCTAAAACTATCTATCCCTTCAGCTTTAATGTCCAGCTTAGAAATGTGGTCATTTGAAATGTTGGTCCTCGTATCTGGTTTGCTTCCGAATCCAAAACTTGAAACATCAGTCCTGTCAATCAGCCTTAACACATGTGCAATGGCATACATGATTCCCCTGGCATTTAGTGGGGTAGTAAGCACAAGAGTTTCAAATCAATTGGGTGCTGGGCAACCGCAACTAGCGCGTCTAACAGCATGTGTTGCACTTTGCCTTGTTATTACTGAATGCATTGTTGTTGCTGCTCTCATGATATTGGGTCGAAGAGTTTGGGGCTACTGTTACAGCAGTGAAAAAGTAGTTGTGAACTATGCTGCAGAAATATTGATTTTAGTTGCTATATCCCACTTTGTTGATGGGCCTCAGTCTGTTCTGTCAGGTATCATAAGAGGAAGTGGGCAGCAGAAGATTGGTGCATATGTTAATCTGGGAGCTTATTATCTTATCGGCATTCCTACTGGAGTATTGTTTGCTTTTAACCTCCACATCGGAGGGAAGGGTCTTTGCATAGGAATTATCCTGGCGCTATGTGTGCAGTCACTGTTTCTTGCAATTATAGTCCTACGGACTGATTGGGATAAAGAATTTTATCAATCAAGGCTTGAAGGCAGCCGCACCAGCCATTTATTTAAAAATAATAATAATAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

475

Amino Acids

51.83

Weight (kDa)

8.32

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 43 - 190 3.6e-33 MatE
MatE PF01554 251 - 413 4.2e-25 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 298, 576
AccII CGCG 1 cut(s) 961
AciI CCGC 4 cut(s) 143, 258, 950, 1387
AclWI GGATC 3 cut(s) 392, 601, 641
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 2 cut(s) 723, 1358
AcuI CTGAAG 3 cut(s) 186, 365, 738
AflIII ACRYGT 1 cut(s) 866
AgsI TTSAA 7 cut(s) 32, 71, 183, 791, 836, 927, 1379
AjnI CCWGG 2 cut(s) 891, 1293
AluBI AGCT 6 cut(s) 127, 163, 219, 758, 771, 1208
AluI AGCT 6 cut(s) 127, 163, 219, 758, 771, 1208
AlwI GGATC 3 cut(s) 392, 601, 641
AlwNI CAGNNNCTG 3 cut(s) 209, 536, 1315
AoxI GGCC 3 cut(s) 140, 711, 1133
ApeKI GCWGC 7 cut(s) 124, 416, 605, 1016, 1088, 1174, 1383
ApoI RAATTY 2 cut(s) 723, 1358
AspLEI GCGC 3 cut(s) 242, 961, 1300
AspS9I GGNCC 2 cut(s) 799, 1133
AsuHPI GGTGA 1 cut(s) 616
AsuNHI GCTAGC 1 cut(s) 524
AvaII GGWCC 1 cut(s) 799
BbvI GCAGC 7 cut(s) 136, 403, 592, 1003, 1075, 1186, 1395
BccI CCATC 2 cut(s) 629, 1124
BciT130I CCWGG 2 cut(s) 893, 1295
BfaI CTAG 6 cut(s) 146, 174, 483, 525, 597, 956
BfmI CTRYAG 2 cut(s) 417, 1089
BfoI RGCGCY 2 cut(s) 243, 1301
BisI GCNGC 9 cut(s) 125, 143, 417, 606, 1017, 1089, 1175, 1384, 1387
BlsI GCNGC 9 cut(s) 126, 144, 418, 607, 1018, 1090, 1176, 1385, 1388
Bme1390I CCNGG 2 cut(s) 893, 1295
Bme18I GGWCC 1 cut(s) 799
BmgT120I GGNCC 2 cut(s) 799, 1133
BmrFI CCNGG 2 cut(s) 893, 1295
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 279
BmtI GCTAGC 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 236
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpmI CTGGAG 1 cut(s) 1254
Bpu10I CCTNAGC 1 cut(s) 388
BpuEI CTTGAG 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 243, 589, 718, 891
Bse1I ACTGG 4 cut(s) 214, 231, 700, 1237
Bse3DI GCAATG 3 cut(s) 321, 511, 879
BseBI CCWGG 2 cut(s) 893, 1295
BseDI CCNNGG 4 cut(s) 243, 589, 718, 891
BseGI GGATG 2 cut(s) 10, 294
BseMI GCAATG 3 cut(s) 321, 511, 879
BseMII CTCAG 1 cut(s) 1151
BseNI ACTGG 4 cut(s) 214, 231, 700, 1237
BseXI GCAGC 7 cut(s) 136, 403, 592, 1003, 1075, 1186, 1395
BseYI CCCAGC 1 cut(s) 941
BsgI GTGCAG 2 cut(s) 533, 1328
Bsh1236I CGCG 1 cut(s) 961
BshFI GGCC 3 cut(s) 142, 713, 1135
BsmI GAATGC 2 cut(s) 1007, 1224
BsnI GGCC 3 cut(s) 142, 713, 1135
Bsp143I GATC 4 cut(s) 25, 397, 593, 633
Bsp19I CCATGG 1 cut(s) 718
BspACI CCGC 4 cut(s) 143, 258, 950, 1387
BspANI GGCC 3 cut(s) 142, 713, 1135
BspCNI CTCAG 1 cut(s) 1150
BspFNI CGCG 1 cut(s) 961
BspHI TCATGA 1 cut(s) 1023
BspMAI CTGCAG 2 cut(s) 421, 1093
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 3 cut(s) 392, 601, 641
BsrDI GCAATG 3 cut(s) 321, 511, 879
BsrI ACTGG 4 cut(s) 214, 231, 700, 1237
BssECI CCNNGG 4 cut(s) 243, 589, 718, 891
BssMI GATC 4 cut(s) 25, 397, 593, 633
BssNAI GTATAC 2 cut(s) 299, 577
BssT1I CCWWGG 3 cut(s) 243, 589, 718
Bst1107I GTATAC 2 cut(s) 299, 577
Bst2UI CCWGG 2 cut(s) 893, 1295
Bst4CI ACNGT 4 cut(s) 39, 282, 1055, 1317
Bst6I CTCTTC 1 cut(s) 1033
BstAPI GCANNNNNTGC 1 cut(s) 977
BstC8I GCNNGC 3 cut(s) 171, 370, 526
BstDEI CTNAG 3 cut(s) 388, 772, 1137
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 2 cut(s) 10, 294
BstFNI CGCG 1 cut(s) 961
BstH2I RGCGCY 2 cut(s) 243, 1301
BstHHI GCGC 3 cut(s) 242, 961, 1300
BstKTI GATC 4 cut(s) 28, 400, 596, 636
BstMBI GATC 4 cut(s) 25, 397, 593, 633
BstMWI GCNNNNNNNGC 6 cut(s) 185, 611, 958, 977, 1306, 1392
BstNI CCWGG 2 cut(s) 893, 1295
BstNSI RCATGY 3 cut(s) 65, 870, 975
BstSCI CCNGG 2 cut(s) 891, 1293
BstSFI CTRYAG 2 cut(s) 417, 1089
BstUI CGCG 1 cut(s) 961
BstV1I GCAGC 7 cut(s) 136, 403, 592, 1003, 1075, 1186, 1395
BstX2I RGATCY 2 cut(s) 397, 593
BstYI RGATCY 2 cut(s) 397, 593
BstZ17I GTATAC 2 cut(s) 299, 577
BsuRI GGCC 3 cut(s) 142, 713, 1135
BtgI CCRYGG 1 cut(s) 718
BtsCI GGATG 2 cut(s) 10, 294
BtsI GCAGTG 1 cut(s) 1069
BtsIMutI CAGTG 5 cut(s) 35, 207, 389, 1069, 1313
Cac8I GCNNGC 3 cut(s) 171, 370, 526
CaiI CAGNNNCTG 3 cut(s) 209, 536, 1315
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 3 cut(s) 242, 961, 1300
Cfr13I GGNCC 2 cut(s) 799, 1133
CseI GACGC 1 cut(s) 950
CviAII CATG 8 cut(s) 62, 479, 690, 719, 867, 883, 972, 1024
DdeI CTNAG 3 cut(s) 388, 772, 1137
DpnI GATC 4 cut(s) 27, 399, 595, 635
DpnII GATC 4 cut(s) 25, 397, 593, 633
DraI TTTAAA 1 cut(s) 1405
EaeI YGGCCR 1 cut(s) 140
Eam1104I CTCTTC 1 cut(s) 1033
EarI CTCTTC 1 cut(s) 1033
Eco130I CCWWGG 3 cut(s) 243, 589, 718
Eco147I AGGCCT 1 cut(s) 713
Eco32I GATATC 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 799
Eco57I CTGAAG 3 cut(s) 186, 365, 738
EcoRI GAATTC 1 cut(s) 723
EcoRII CCWGG 2 cut(s) 891, 1293
EcoRV GATATC 1 cut(s) 408
EcoT14I CCWWGG 3 cut(s) 243, 589, 718
EcoT22I ATGCAT 2 cut(s) 643, 1007
ErhI CCWWGG 3 cut(s) 243, 589, 718
FaeI CATG 8 cut(s) 65, 482, 693, 722, 870, 886, 975, 1027
FalI AAGNNNNNCTT 2 cut(s) 698, 730
FatI CATG 8 cut(s) 61, 478, 689, 718, 866, 882, 971, 1023
FauNDI CATATG 2 cut(s) 303, 1192
FblI GTMKAC 2 cut(s) 298, 576
Fnu4HI GCNGC 9 cut(s) 125, 143, 417, 606, 1017, 1089, 1175, 1384, 1387
FokI GGATG 2 cut(s) 17, 301
Fsp4HI GCNGC 9 cut(s) 125, 143, 417, 606, 1017, 1089, 1175, 1384, 1387
FspBI CTAG 6 cut(s) 146, 174, 483, 525, 597, 956
GlaI GCGC 3 cut(s) 241, 960, 1299
GluI GCNGC 9 cut(s) 125, 143, 417, 606, 1017, 1089, 1175, 1384, 1387
GsaI CCCAGC 1 cut(s) 945
GsuI CTGGAG 1 cut(s) 1254
HaeII RGCGCY 2 cut(s) 243, 1301
HaeIII GGCC 3 cut(s) 142, 713, 1135
HgaI GACGC 1 cut(s) 950
HhaI GCGC 3 cut(s) 242, 961, 1300
Hin1II CATG 8 cut(s) 65, 482, 693, 722, 870, 886, 975, 1027
Hin6I GCGC 3 cut(s) 240, 959, 1298
HinP1I GCGC 3 cut(s) 240, 959, 1298
HinfI GANTC 9 cut(s) 32, 43, 71, 84, 486, 511, 699, 823, 886
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 3 cut(s) 299, 577, 1081
Hpy188I TCNGA 4 cut(s) 288, 665, 822, 1265
Hpy188III TCNNGA 8 cut(s) 29, 56, 75, 88, 395, 401, 597, 1024
Hpy8I GTNNAC 3 cut(s) 299, 577, 1081
HpyAV CCTTC 4 cut(s) 724, 762, 1264, 1373
HpyCH4III ACNGT 4 cut(s) 39, 282, 1055, 1317
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 611, 958, 977, 1306, 1392
HpyF3I CTNAG 3 cut(s) 388, 772, 1137
Hsp92II CATG 8 cut(s) 65, 482, 693, 722, 870, 886, 975, 1027
HspAI GCGC 3 cut(s) 240, 959, 1298
Kzo9I GATC 4 cut(s) 25, 397, 593, 633
LmnI GCTCC 2 cut(s) 272, 1205
Lsp1109I GCAGC 7 cut(s) 136, 403, 592, 1003, 1075, 1186, 1395
LweI GCATC 1 cut(s) 279
MaeI CTAG 6 cut(s) 146, 174, 483, 525, 597, 956
MaeIII GTNAC 3 cut(s) 205, 1055, 1311
MalI GATC 4 cut(s) 27, 399, 595, 635
MboI GATC 4 cut(s) 25, 397, 593, 633
MboII GAAGA 6 cut(s) 23, 26, 130, 371, 1050, 1192
MfeI CAATTG 1 cut(s) 932
MflI RGATCY 2 cut(s) 397, 593
MluCI AATT 7 cut(s) 49, 351, 723, 932, 1287, 1326, 1358
Mph1103I ATGCAT 2 cut(s) 643, 1007
MseI TTAA 6 cut(s) 134, 761, 861, 1197, 1251, 1404
MspA1I CMGCKG 1 cut(s) 127
MspR9I CCNGG 2 cut(s) 893, 1295
MunI CAATTG 1 cut(s) 932
Mva1269I GAATGC 2 cut(s) 1007, 1224
MvaI CCWGG 2 cut(s) 893, 1295
MvnI CGCG 1 cut(s) 961
MwoI GCNNNNNNNGC 6 cut(s) 185, 611, 958, 977, 1306, 1392
NcoI CCATGG 1 cut(s) 718
NdeI CATATG 2 cut(s) 303, 1192
NdeII GATC 4 cut(s) 25, 397, 593, 633
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 8 cut(s) 65, 482, 693, 722, 870, 886, 975, 1027
NmuCI GTSAC 2 cut(s) 205, 1311
NsiI ATGCAT 2 cut(s) 643, 1007
NspI RCATGY 3 cut(s) 65, 870, 975
PagI TCATGA 1 cut(s) 1023
PceI AGGCCT 1 cut(s) 713
PciI ACATGT 1 cut(s) 866
PctI GAATGC 2 cut(s) 1007, 1224
PfeI GAWTC 9 cut(s) 32, 43, 71, 84, 486, 511, 699, 823, 886
PkrI GCNGC 9 cut(s) 126, 144, 418, 607, 1018, 1090, 1176, 1385, 1388
PscI ACATGT 1 cut(s) 866
Psp6I CCWGG 2 cut(s) 891, 1293
PspFI CCCAGC 1 cut(s) 941
PspGI CCWGG 2 cut(s) 891, 1293
PspPI GGNCC 2 cut(s) 799, 1133
PstI CTGCAG 2 cut(s) 421, 1093
PstNI CAGNNNCTG 3 cut(s) 209, 536, 1315
PsuI RGATCY 2 cut(s) 397, 593
PvuII CAGCTG 1 cut(s) 127
SaqAI TTAA 6 cut(s) 134, 761, 861, 1197, 1251, 1404
SatI GCNGC 9 cut(s) 125, 143, 417, 606, 1017, 1089, 1175, 1384, 1387
Sau3AI GATC 4 cut(s) 25, 397, 593, 633
Sau96I GGNCC 2 cut(s) 799, 1133
ScrFI CCNGG 2 cut(s) 893, 1295
SfaNI GCATC 1 cut(s) 279
SfcI CTRYAG 2 cut(s) 417, 1089
SinI GGWCC 1 cut(s) 799
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 7 cut(s) 49, 351, 723, 932, 1287, 1326, 1358
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 4 cut(s) 143, 258, 950, 1387
SspI AATATT 2 cut(s) 376, 1098
SspMI CTAG 6 cut(s) 146, 174, 483, 525, 597, 956
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 2 cut(s) 891, 1293
StyI CCWWGG 3 cut(s) 243, 589, 718
TaaI ACNGT 4 cut(s) 39, 282, 1055, 1317
TaqI TCGA 2 cut(s) 74, 1036
TasI AATT 7 cut(s) 49, 351, 723, 932, 1287, 1326, 1358
TauI GCSGC 2 cut(s) 145, 1389
TfiI GAWTC 9 cut(s) 32, 43, 71, 84, 486, 511, 699, 823, 886
Tru1I TTAA 6 cut(s) 134, 761, 861, 1197, 1251, 1404
Tru9I TTAA 6 cut(s) 134, 761, 861, 1197, 1251, 1404
TscAI CASTG 5 cut(s) 42, 214, 389, 1069, 1320
TseFI GTSAC 2 cut(s) 205, 1311
TseI GCWGC 7 cut(s) 124, 416, 605, 1016, 1088, 1174, 1383
Tsp45I GTSAC 2 cut(s) 205, 1311
TspDTI ATGAA 2 cut(s) 140, 183
TspGWI ACGGA 1 cut(s) 1354
TspRI CASTG 5 cut(s) 42, 214, 389, 1069, 1320
VpaK11BI GGWCC 1 cut(s) 799
XapI RAATTY 2 cut(s) 723, 1358
XbaI TCTAGA 1 cut(s) 596
XceI RCATGY 3 cut(s) 65, 870, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 1127
XmiI GTMKAC 2 cut(s) 298, 576
XspI CTAG 6 cut(s) 146, 174, 483, 525, 597, 956
Zsp2I ATGCAT 2 cut(s) 643, 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.