Rorug04G0043300

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000521
Physical Location & Seq
Forward (+)
6778795 .. 6779343
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0043300.1

Sequence Viewer

Length: 549 bp
ATGAACCAGGCTGAATTTGCACTTGACTTCGGCGGCCAGTTTGAGCAGTTGAAGACAGGGCTTGATCTCACCAAGGCCTTACTTGCAGACACGGAGAGTCTCAACCACAAGAACAACATTGTCAAGGCAGCATTATTTGAGCTGAGAGAAGTCATCTACAAAGCCGACGATGTACTCACTGATTGCTTGGTTAGAGATGAATACAGAAAGGATGGATCTTGTTCCGGCTCCTTGTTTCACGATCCATTCTTCCTGCATCGTACGGGAAAGAAATTGAAAGACATCAATTTGCGTATGAAGGAGATAGAGCAGACCTTGGGCAAGTTTTTGAGGGCTCCTGATAACATCCATAGAGAAGATGCATACCAAGTTAGGGTGATGGTTTCACAGGACTGGAATCCAACTGAGATAATTGGGTTGGATAGCGATGTGGAGAAGATTAAGGGTTGGCTTTTCGACACTAGCAAGGTACTTTCCCACGTAGGCATTGTGGGAATGGGAGGCTTGGGGAAAACCACCATTGCTCAAAAGATATTTCATGATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

182

Amino Acids

20.61

Weight (kDa)

5.86

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 3 - 74 1.6e-10 Rx N-terminal domain
NB-ARC PF00931 142 - 181 1.1e-08 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 33
AclWI GGATC 2 cut(s) 223, 236
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 1 cut(s) 14
AfaI GTAC 3 cut(s) 174, 262, 471
AfiI CCNNNNNNNGG 1 cut(s) 373
AgsI TTSAA 2 cut(s) 52, 277
AjnI CCWGG 1 cut(s) 6
AluBI AGCT 1 cut(s) 142
AluI AGCT 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 2 cut(s) 223, 236
AoxI GGCC 2 cut(s) 34, 75
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 1 cut(s) 14
AsuHPI GGTGA 2 cut(s) 61, 388
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 140
BccI CCATC 2 cut(s) 206, 373
BciT130I CCWGG 1 cut(s) 8
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 462
BisI GCNGC 2 cut(s) 34, 129
BlsI GCNGC 2 cut(s) 35, 130
Bme1390I CCNGG 1 cut(s) 8
BmiI GGNNCC 2 cut(s) 229, 336
BmrFI CCNGG 1 cut(s) 8
BmsI GCATC 2 cut(s) 265, 349
BpiI GAAGAC 1 cut(s) 59
BsaAI YACGTR 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 72, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 373
Bse1I ACTGG 2 cut(s) 37, 398
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 72, 315
BseGI GGATG 2 cut(s) 217, 345
BseLI CCNNNNNNNGG 1 cut(s) 373
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 134, 396
BseNI ACTGG 2 cut(s) 37, 398
BseXI GCAGC 1 cut(s) 140
BshFI GGCC 2 cut(s) 36, 77
BsiSI CCGG 1 cut(s) 225
BsiWI CGTACG 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 373
BsmAI GTCTC 1 cut(s) 104
BsnI GGCC 2 cut(s) 36, 77
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 4 cut(s) 64, 215, 241, 541
BspACI CCGC 1 cut(s) 33
BspANI GGCC 2 cut(s) 36, 77
BspCNI CTCAG 2 cut(s) 135, 397
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 229, 336
BspPI GGATC 2 cut(s) 223, 236
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 2 cut(s) 37, 398
BssECI CCNNGG 2 cut(s) 72, 315
BssMI GATC 4 cut(s) 64, 215, 241, 541
BssT1I CCWWGG 2 cut(s) 72, 315
Bst2UI CCWGG 1 cut(s) 8
BstBAI YACGTR 1 cut(s) 481
BstDEI CTNAG 2 cut(s) 143, 405
BstF5I GGATG 2 cut(s) 217, 345
BstKTI GATC 4 cut(s) 67, 218, 244, 544
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 4 cut(s) 64, 215, 241, 541
BstMWI GCNNNNNNNGC 2 cut(s) 17, 83
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 2 cut(s) 36, 77
BtgZI GCGATG 1 cut(s) 441
BtsCI GGATG 2 cut(s) 217, 345
BtsIMutI CAGTG 1 cut(s) 177
CciI TCATGA 1 cut(s) 538
Csp6I GTAC 3 cut(s) 173, 261, 470
CviAII CATG 1 cut(s) 539
CviQI GTAC 3 cut(s) 173, 261, 470
DdeI CTNAG 2 cut(s) 143, 405
DpnI GATC 4 cut(s) 66, 217, 243, 543
DpnII GATC 4 cut(s) 64, 215, 241, 541
EaeI YGGCCR 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 72, 315
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 2 cut(s) 72, 315
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 2 cut(s) 72, 315
FaeI CATG 1 cut(s) 542
FaiI YATR 5 cut(s) 296, 351, 364, 540, 547
FatI CATG 1 cut(s) 538
Fnu4HI GCNGC 2 cut(s) 34, 129
FokI GGATG 2 cut(s) 224, 332
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 2 cut(s) 34, 129
FspBI CTAG 1 cut(s) 462
GluI GCNGC 2 cut(s) 34, 129
HaeIII GGCC 2 cut(s) 36, 77
HapII CCGG 1 cut(s) 225
Hin1II CATG 1 cut(s) 542
HinfI GANTC 2 cut(s) 97, 397
HpaII CCGG 1 cut(s) 225
HphI GGTGA 2 cut(s) 61, 388
Hpy188III TCNNGA 3 cut(s) 239, 338, 539
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 4 cut(s) 20, 86, 256, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 83
HpyF3I CTNAG 2 cut(s) 143, 405
HpySE526I ACGT 1 cut(s) 480
Hsp92II CATG 1 cut(s) 542
Kzo9I GATC 4 cut(s) 64, 215, 241, 541
LmnI GCTCC 2 cut(s) 233, 340
LpnPI CCDG 8 cut(s) 20, 42, 50, 238, 266, 351, 374, 379
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 2 cut(s) 265, 349
MaeI CTAG 1 cut(s) 462
MaeII ACGT 1 cut(s) 480
MalI GATC 4 cut(s) 66, 217, 243, 543
MboI GATC 4 cut(s) 64, 215, 241, 541
MboII GAAGA 4 cut(s) 64, 241, 368, 448
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 4 cut(s) 14, 272, 286, 411
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 2 cut(s) 399, 425
MnlI CCTC 2 cut(s) 324, 494
Mph1103I ATGCAT 1 cut(s) 364
MseI TTAA 1 cut(s) 441
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 8
MwoI GCNNNNNNNGC 2 cut(s) 17, 83
NdeII GATC 4 cut(s) 64, 215, 241, 541
NlaIII CATG 1 cut(s) 542
NlaIV GGNNCC 2 cut(s) 229, 336
NsiI ATGCAT 1 cut(s) 364
PagI TCATGA 1 cut(s) 538
PceI AGGCCT 1 cut(s) 77
PfeI GAWTC 1 cut(s) 397
Pfl23II CGTACG 1 cut(s) 260
PkrI GCNGC 2 cut(s) 35, 130
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Ppu21I YACGTR 1 cut(s) 481
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspLI CGTACG 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 229, 336
PsuI RGATCY 1 cut(s) 215
RsaI GTAC 3 cut(s) 174, 262, 471
RsaNI GTAC 3 cut(s) 173, 261, 470
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 2 cut(s) 34, 129
Sau3AI GATC 4 cut(s) 64, 215, 241, 541
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 8
SduI GDGCHC 1 cut(s) 337
SetI ASST 4 cut(s) 144, 317, 471, 483
SfaNI GCATC 2 cut(s) 265, 349
Sse9I AATT 4 cut(s) 14, 272, 286, 411
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 1 cut(s) 33
SspMI CTAG 1 cut(s) 462
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 6
StyI CCWWGG 2 cut(s) 72, 315
TaiI ACGT 1 cut(s) 483
TaqI TCGA 1 cut(s) 456
TasI AATT 4 cut(s) 14, 272, 286, 411
TatI WGTACW 1 cut(s) 172
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 1 cut(s) 184
TseI GCWGC 1 cut(s) 128
TspDTI ATGAA 4 cut(s) 17, 213, 311, 527
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 14
XspI CTAG 1 cut(s) 462
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.