Rh4BG111900

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000521
Physical Location & Seq
Reverse (-)
18883776 .. 18886672
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG111900.1

Sequence Viewer

Length: 1470 bp
ATGGCTGACGAAGAACAATCTGATGGTCTTGAATCACCCTTGATTCCAGTTCAGCCCCAACAACATGCATTACAATCAACAAGGGGACAATTCACGAAAGGCGAATTGTTCGGGGAACTGAAGAAGCAGTTATTGTTGGCAGGGCCGCTGGTATCATCAAATTTCTTGTTGTTTGGTATGCAGGTTATTTCAGTCATGTATGTTGGTCATCTTGGGGAGCTATCACTTGCAGGAGCTTCTATGGCCACTTCATTTGCATCTGTGACTGGTTTGAGCTTGATAATAGGAATGGGTAGCGCATTAGACACATTTTGCGGGCAGTCATATGGAGCAAAACAGTATCATATGCTTGGGATACACTTGCAGAGGGCAATGCTTGTTCTTCTGCTGGCCTGCATTCCTATTGCAACTATATGGTTCAATGCGGGCTACATTCTTCAATCCTTGGGTCAAGATCCAGAAATTGCCGCAGCTGCTGGAAACTATGCTCGTTTCTTGATACCTTGCATTTTTGCTTACGCAGTCCAACAATGTCATTCTAGATTCTTGCAAACCCAAAGCAATGTGGTTCCTATGATAGTTAGCACTGGCACAGCAACACTAGTACACTTGCTTGTCTGTTGGCTTCTGGTATATAAGACAAGCCTTGGATATAGAGGAGCTGCTGTCGCAATCTCCATCTCATATTGGATCAATGCGTTGCTCTTGGTTGTTTATATCAGAGTTTCTCCCTCTTGTAAACACACATGGACTGGATTTTCAAAGGAGGCATTTCATGGAATTCCCAACTTCATAAAATTATCTATCCCTTCAGCTATAATGATCAGCCTAGAAATCTGGTCATTCGAATTGATGGTTCTCTTATCCGGTTTTCTTCCAAATCCACAGCTTGAAACCTCAGTGCTATCAATCAGCCTTAACACATGCTCCATGATATACATGATTCCTCTTGCATTCGCCGCTGCAGCAAGCACAAGAGTTTCAAATCAACTGGGTGCCGGGCAACCACGACTTGCTCGTCTAGCAGTAGGTGTTGCACTATGCATTGTTGTTACCGAAGGTATTATGGCTGCTGCTATACTGATACTGGGTCGAAAAGTTTGGGGATACTGTTACAGCAGTGAAAAGGAAGTGATCAATTATGTTGGACAAATGCTTATTTTGGTTGCGGTATCCCACTTTTTTGATGGACTTCAATCTGTGCTTTCAGGTATCATAAGAGGAAGTGGACAGCAGAAGATTGGGGCATATGTTAATTTGGGAGCTTATTATCTTATGGGAATTCCTACTGCAATATTATTAGCATTTGTATTCCACATTGGAGGAAAGGGTCTTTGGATGGGAATCATTGTTGCGCTATTTGTGCAAGCACTGTCTCTTGCAATCATAATCTTATTCACAGACTGGGAAAAAGAAGTGAAGAAAGCTTCTGATAGAGTATACAACACACCAACCGCTTCTGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

489

Amino Acids

52.79

Weight (kDa)

8.29

Isoelectric Point (pI)

29.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 49 - 209 1.4e-38 MatE
MatE PF01554 270 - 432 3.8e-26 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000521)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_4g09990 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860 FvH4_7g32860
malus_domestica MD11G1148000.v1.1 MD13G1266200.v1.1 MD14G1231800.v1.1
prunus_persica Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090100_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1 Prupe.1G090300_v2.0.a1
pyrus_communis pycom13g23330
rosa_chinensis RchiOBHm_Chr4g0403401 RchiOBHm_Chr4g0403411 RchiOBHm_Chr4g0403421 RchiOBHm_Chr4g0403461 RchiOBHm_Chr4g0403631 RchiOBHm_Chr7g0223601 RchiOBHm_Chr7g0238161
rosa_laevigata RLG00000000937 RLG00000001957 RLG00000008965 RLG00000008975 RLG00000008976 RLG00000008978
rosa_multiflora Rmu_co8335743.1_g000001 Rmu_sc0005734.1_g000001 Rmu_sc0007727.1_g000011 Rmu_sc0011775.1_g000002 Rmu_sc0011775.1_g000005 Rmu_sc0028062.1_g000002
rosa_roxburghii Rroxscaffold_3G00223300 Rroxscaffold_5G00348050 Rroxscaffold_5G00348060 Rroxscaffold_5G00348120 Rroxscaffold_5G00348240
rosa_rugosa Rorug04G0042300 Rorug04G0042400 Rorug04G0042500 Rorug04G0042600.1 Rorug04G0042700 Rorug04G0042800 Rorug04G0042900 Rorug04G0043000 Rorug04G0043100 Rorug04G0043200 Rorug04G0043300 Rorug04G0043400 Rorug04G0044700 Rorug07G0310600 Rorug07G0310700 Rorug07G0310700
rosa_samantha Rh4AG118700 Rh4AG118800 Rh4AG118900 Rh4AG119000 Rh4BG111600 Rh4BG111800 Rh4BG111900 Rh4BG112900 Rh4CG126600 Rh4CG126900 Rh4CG127800 Rh4DG111300 Rh4DG111400 Rh4DG111500 Rh7AG466500 Rh7BG352200 Rh7BG436900 Rh7CG484200 Rh7DG452600
rosa_wichuraiana Rw4G009630 Rw4G009640 Rw4G009650 Rw4G009710 Rw7G038620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1017
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 995
AccI GTMKAC 1 cut(s) 1440
AciI CCGC 7 cut(s) 146, 315, 425, 468, 960, 1169, 1455
AclWI GGATC 2 cut(s) 449, 698
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 3 cut(s) 160, 780, 1281
AcuI CTGAAG 2 cut(s) 140, 795
AfaI GTAC 1 cut(s) 606
AgsI TTSAA 7 cut(s) 32, 421, 440, 762, 893, 984, 1196
AhlI ACTAGT 1 cut(s) 601
AjuI GAANNNNNNNTTGG 2 cut(s) 1318, 1350
AluBI AGCT 9 cut(s) 220, 236, 276, 473, 662, 815, 889, 1265, 1427
AluI AGCT 9 cut(s) 220, 236, 276, 473, 662, 815, 889, 1265, 1427
Alw26I GTCTC 1 cut(s) 1380
AlwI GGATC 2 cut(s) 449, 698
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 3 cut(s) 143, 243, 390
ApeKI GCWGC 7 cut(s) 470, 473, 662, 962, 965, 1070, 1073
ApoI RAATTY 3 cut(s) 160, 780, 1281
AspLEI GCGC 2 cut(s) 299, 1357
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 1000
AsuHPI GGTGA 1 cut(s) 27
AsuII TTCGAA 1 cut(s) 846
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 1 cut(s) 995
BbvI GCAGC 7 cut(s) 460, 482, 649, 949, 977, 1057, 1060
BccI CCATC 5 cut(s) 17, 686, 847, 1181, 1333
BciVI GTATCC 3 cut(s) 348, 1100, 1183
BclI TGATCA 2 cut(s) 822, 1134
BcnI CCSGG 1 cut(s) 1000
BcoDI GTCTC 1 cut(s) 1380
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 4 cut(s) 540, 602, 830, 1022
BfmI CTRYAG 1 cut(s) 963
BfuAI ACCTGC 1 cut(s) 172
BfuI GTATCC 3 cut(s) 348, 1100, 1183
Bme1390I CCNGG 1 cut(s) 1000
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 570, 997
BmrFI CCNGG 1 cut(s) 1000
BmrI ACTGGG 3 cut(s) 1001, 1097, 1414
BmsI GCATC 2 cut(s) 266, 1453
BmuI ACTGGG 3 cut(s) 1001, 1097, 1414
Bpu14I TTCGAA 1 cut(s) 846
BpuMI CCSGG 1 cut(s) 1000
BsaBI GATNNNNATC 1 cut(s) 1343
BsaJI CCNNGG 2 cut(s) 444, 646
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 4 cut(s) 651, 681, 911, 941
Bse1I ACTGG 7 cut(s) 47, 271, 592, 757, 996, 1092, 1409
Bse3DI GCAATG 2 cut(s) 378, 568
Bse8I GATNNNNATC 1 cut(s) 1343
BseDI CCNNGG 2 cut(s) 444, 646
BseGI GGATG 1 cut(s) 1344
BseJI GATNNNNATC 1 cut(s) 1343
BseMI GCAATG 2 cut(s) 378, 568
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 7 cut(s) 47, 271, 592, 757, 996, 1092, 1409
BseRI GAGGAG 1 cut(s) 672
BseXI GCAGC 7 cut(s) 460, 482, 649, 949, 977, 1057, 1060
BshFI GGCC 3 cut(s) 145, 245, 392
BshNI GGYRCC 1 cut(s) 995
BsiSI CCGG 2 cut(s) 867, 999
BslFI GGGAC 1 cut(s) 99
BsmAI GTCTC 1 cut(s) 1380
BsmFI GGGAC 1 cut(s) 99
BsmI GAATGC 2 cut(s) 396, 953
BsnI GGCC 3 cut(s) 145, 245, 392
Bsp119I TTCGAA 1 cut(s) 846
Bsp143I GATC 4 cut(s) 454, 690, 822, 1134
BspACI CCGC 7 cut(s) 146, 315, 425, 468, 960, 1169, 1455
BspANI GGCC 3 cut(s) 145, 245, 392
BspCNI CTCAG 1 cut(s) 911
BspLI GGNNCC 2 cut(s) 570, 997
BspMAI CTGCAG 1 cut(s) 967
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 2 cut(s) 449, 698
BspT104I TTCGAA 1 cut(s) 846
BspT107I GGYRCC 1 cut(s) 995
BsrDI GCAATG 2 cut(s) 378, 568
BsrI ACTGG 7 cut(s) 47, 271, 592, 757, 996, 1092, 1409
BssECI CCNNGG 2 cut(s) 444, 646
BssMI GATC 4 cut(s) 454, 690, 822, 1134
BssNAI GTATAC 1 cut(s) 1441
BssT1I CCWWGG 2 cut(s) 444, 646
Bst1107I GTATAC 1 cut(s) 1441
Bst4CI ACNGT 3 cut(s) 339, 1112, 1374
BstBI TTCGAA 1 cut(s) 846
BstC8I GCNNGC 6 cut(s) 317, 390, 394, 427, 970, 1368
BstDEI CTNAG 1 cut(s) 898
BstF5I GGATG 1 cut(s) 1344
BstHHI GCGC 2 cut(s) 299, 1357
BstKTI GATC 4 cut(s) 457, 693, 825, 1137
BstMAI GTCTC 1 cut(s) 1380
BstMBI GATC 4 cut(s) 454, 690, 822, 1134
BstMWI GCNNNNNNNGC 8 cut(s) 242, 473, 668, 959, 965, 1022, 1363, 1463
BstNSI RCATGY 2 cut(s) 68, 927
BstSCI CCNGG 1 cut(s) 998
BstSFI CTRYAG 1 cut(s) 963
BstV1I GCAGC 7 cut(s) 460, 482, 649, 949, 977, 1057, 1060
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BstZ17I GTATAC 1 cut(s) 1441
BsuI GTATCC 3 cut(s) 348, 1100, 1183
BsuRI GGCC 3 cut(s) 145, 245, 392
BtsCI GGATG 1 cut(s) 1344
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 4 cut(s) 585, 906, 1126, 1370
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 6 cut(s) 317, 390, 394, 427, 970, 1368
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 2 cut(s) 299, 1357
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 605
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 7 cut(s) 65, 196, 747, 776, 924, 931, 940
CviQI GTAC 1 cut(s) 605
DdeI CTNAG 1 cut(s) 898
DpnI GATC 4 cut(s) 456, 692, 824, 1136
DpnII GATC 4 cut(s) 454, 690, 822, 1134
DrdI GACNNNNNNGTC 1 cut(s) 1017
DseDI GACNNNNNNGTC 1 cut(s) 1017
EaeI YGGCCR 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 444, 646
Eco57I CTGAAG 2 cut(s) 140, 795
EcoRI GAATTC 2 cut(s) 780, 1281
EcoT14I CCWWGG 2 cut(s) 444, 646
EcoT22I ATGCAT 3 cut(s) 70, 1046, 1468
ErhI CCWWGG 2 cut(s) 444, 646
FaeI CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
FaqI GGGAC 1 cut(s) 99
FatI CATG 7 cut(s) 64, 195, 746, 775, 923, 930, 939
FauI CCCGC 2 cut(s) 308, 418
FauNDI CATATG 3 cut(s) 325, 345, 1249
FbaI TGATCA 2 cut(s) 822, 1134
FblI GTMKAC 1 cut(s) 1440
FokI GGATG 1 cut(s) 1351
FspBI CTAG 4 cut(s) 540, 602, 830, 1022
GlaI GCGC 2 cut(s) 298, 1356
HaeIII GGCC 3 cut(s) 145, 245, 392
HapII CCGG 2 cut(s) 867, 999
HhaI GCGC 2 cut(s) 299, 1357
Hin1II CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
Hin6I GCGC 2 cut(s) 297, 1355
HinP1I GCGC 2 cut(s) 297, 1355
HindIII AAGCTT 1 cut(s) 1425
HinfI GANTC 5 cut(s) 32, 43, 543, 943, 1344
HpaII CCGG 2 cut(s) 867, 999
HphI GGTGA 1 cut(s) 27
Hpy166II GTNNAC 4 cut(s) 607, 740, 1229, 1441
Hpy188I TCNGA 4 cut(s) 22, 722, 1432, 1462
Hpy188III TCNNGA 6 cut(s) 29, 94, 452, 458, 496, 540
Hpy8I GTNNAC 4 cut(s) 607, 740, 1229, 1441
HpyAV CCTTC 2 cut(s) 819, 1052
HpyCH4III ACNGT 3 cut(s) 339, 1112, 1374
HpyF10VI GCNNNNNNNGC 8 cut(s) 242, 473, 668, 959, 965, 1022, 1363, 1463
HpyF3I CTNAG 1 cut(s) 898
Hsp92II CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
HspAI GCGC 2 cut(s) 297, 1355
Ksp22I TGATCA 2 cut(s) 822, 1134
Kzo9I GATC 4 cut(s) 454, 690, 822, 1134
LmnI GCTCC 6 cut(s) 217, 233, 329, 659, 932, 1262
Lsp1109I GCAGC 7 cut(s) 460, 482, 649, 949, 977, 1057, 1060
LweI GCATC 2 cut(s) 266, 1453
MaeI CTAG 4 cut(s) 540, 602, 830, 1022
MaeIII GTNAC 3 cut(s) 262, 1051, 1112
MalI GATC 4 cut(s) 456, 692, 824, 1136
MboI GATC 4 cut(s) 454, 690, 822, 1134
MboII GAAGA 7 cut(s) 23, 133, 374, 428, 866, 1249, 1432
MflI RGATCY 1 cut(s) 454
MlsI TGGCCA 1 cut(s) 245
MluNI TGGCCA 1 cut(s) 245
MmeI TCCRAC 2 cut(s) 550, 1126
MnlI CCTC 8 cut(s) 360, 650, 742, 760, 907, 957, 1214, 1316
Mox20I TGGCCA 1 cut(s) 245
Mph1103I ATGCAT 3 cut(s) 70, 1046, 1468
MscI TGGCCA 1 cut(s) 245
MseI TTAA 2 cut(s) 918, 1254
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 3 cut(s) 148, 473, 962
MspI CCGG 2 cut(s) 867, 999
MspR9I CCNGG 1 cut(s) 1000
Mva1269I GAATGC 2 cut(s) 396, 953
MwoI GCNNNNNNNGC 8 cut(s) 242, 473, 668, 959, 965, 1022, 1363, 1463
NciI CCSGG 1 cut(s) 1000
NdeI CATATG 3 cut(s) 325, 345, 1249
NdeII GATC 4 cut(s) 454, 690, 822, 1134
NlaIII CATG 7 cut(s) 68, 199, 750, 779, 927, 934, 943
NlaIV GGNNCC 2 cut(s) 570, 997
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 3 cut(s) 70, 1046, 1468
NspI RCATGY 2 cut(s) 68, 927
NspV TTCGAA 1 cut(s) 846
PcsI WCGNNNNNNNCGW 1 cut(s) 1015
PctI GAATGC 2 cut(s) 396, 953
PfeI GAWTC 5 cut(s) 32, 43, 543, 943, 1344
PspN4I GGNNCC 2 cut(s) 570, 997
PspPI GGNCC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 967
PstNI CAGNNNCTG 1 cut(s) 476
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
SaqAI TTAA 2 cut(s) 918, 1254
Sau3AI GATC 4 cut(s) 454, 690, 822, 1134
Sau96I GGNCC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 1000
SfaNI GCATC 2 cut(s) 266, 1453
SfcI CTRYAG 1 cut(s) 963
SfuI TTCGAA 1 cut(s) 846
SpeI ACTAGT 1 cut(s) 601
SsiI CCGC 7 cut(s) 146, 315, 425, 468, 960, 1169, 1455
SspI AATATT 1 cut(s) 1296
SspMI CTAG 4 cut(s) 540, 602, 830, 1022
StyD4I CCNGG 1 cut(s) 998
StyI CCWWGG 2 cut(s) 444, 646
TaaI ACNGT 3 cut(s) 339, 1112, 1374
TaqI TCGA 2 cut(s) 846, 1093
TatI WGTACW 1 cut(s) 604
TauI GCSGC 3 cut(s) 148, 470, 962
TfiI GAWTC 5 cut(s) 32, 43, 543, 943, 1344
Tru1I TTAA 2 cut(s) 918, 1254
Tru9I TTAA 2 cut(s) 918, 1254
TscAI CASTG 4 cut(s) 592, 906, 1126, 1377
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 7 cut(s) 470, 473, 662, 962, 965, 1070, 1073
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 3 cut(s) 240, 764, 781
TspRI CASTG 4 cut(s) 592, 906, 1126, 1377
XapI RAATTY 3 cut(s) 160, 780, 1281
XbaI TCTAGA 1 cut(s) 539
XceI RCATGY 2 cut(s) 68, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 1184
XmiI GTMKAC 1 cut(s) 1440
XspI CTAG 4 cut(s) 540, 602, 830, 1022
Zsp2I ATGCAT 3 cut(s) 70, 1046, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.